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Journal articles on the topic 'Assimilation de substrat'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Mary, C., A. Blancard, and M. Quilici. "Carbon assimilation tests: Substrates assimilation profiles." Mycopathologia 102, no. 1 (1988): 3–8. http://dx.doi.org/10.1007/bf00436244.

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2

Renaud, François N. R., Marianne Dutaur, Salah Daoud, et al. "Differentiation of Corynebacterium amycolatum, C. minutissimum, and C. striatum by Carbon Substrate Assimilation Tests." Journal of Clinical Microbiology 36, no. 12 (1998): 3698–702. http://dx.doi.org/10.1128/jcm.36.12.3698-3702.1998.

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We tested the carbon substrate assimilation patterns of 40Corynebacterium amycolatum strains, 19 C. minutissimum strains, 50 C. striatum strains, and 1C. xerosis strain with the Biotype 100 system (bioMérieux, Marcy-l’Étoile, France). Twelve carbon substrates of 99 allowed discrimination among the species tested. Additionally, assimilation of 3 of these 12 carbon substrates (maltose, N -acetyl-d-glucosamine, and phenylacetate) was tested with the API 20 NE identification system (bioMérieux). Since concordant results were observed with the two systems for these three carbon substrates, eithe

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3

Koendjbiharie, Jeroen G., Shuen Hon, Martin Pabst, et al. "The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase." Journal of Biological Chemistry 295, no. 7 (2019): 1867–78. http://dx.doi.org/10.1074/jbc.ra119.011239.

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The genomes of most cellulolytic clostridia do not contain genes annotated as transaldolase. Therefore, for assimilating pentose sugars or for generating C5 precursors (such as ribose) during growth on other (non-C5) substrates, they must possess a pathway that connects pentose metabolism with the rest of metabolism. Here we provide evidence that for this connection cellulolytic clostridia rely on the sedoheptulose 1,7-bisphosphate (SBP) pathway, using pyrophosphate-dependent phosphofructokinase (PPi-PFK) instead of transaldolase. In this reversible pathway, PFK converts sedoheptulose 7-phosph

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4

Urschel, Matthew R., Michael D. Kubo, Tori M. Hoehler, John W. Peters, and Eric S. Boyd. "Carbon Source Preference in Chemosynthetic Hot Spring Communities." Applied and Environmental Microbiology 81, no. 11 (2015): 3834–47. http://dx.doi.org/10.1128/aem.00511-15.

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ABSTRACTRates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73°C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the hot spring communities exhibited rates of DIC assimilation that were greater than those of formate and acetate assimilation, 2 exhibited rates of formate and/or acetate assimilation that exceeded those of DIC assimilation. Overall rates of DIC, formate, and acetate mine

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5

Charteris, A. F., T. D. J. Knowles, K. Michaelides, and R. P. Evershed. "Compound-specific <sup>15</sup>N stable isotope probing of N assimilation by the soil microbial biomass: a new methodological paradigm in soil N cycling." SOIL Discussions 2, no. 2 (2015): 1135–60. http://dx.doi.org/10.5194/soild-2-1135-2015.

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Abstract. A compound-specific nitrogen-15 stable isotope probing (15N-SIP) technique is described which allows investigation of the fate of inorganic- or organic-N amendments to soils. The technique uses gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) to determine the δ15N values of individual amino acids (AAs; determined as N-acetyl, O-isopropyl derivatives) as proxies of biomass protein production. The δ15N values are used together with AA concentrations to quantify N assimilation of 15N-labelled substrates by the soil microbial biomass. The utility of the approach

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6

LaFlamme, Crystal, Christopher R. M. McFarlane, and David Corrigan. "Neoarchean Mantle-derived Magmatism within the Repulse Bay Block, Melville Peninsula, Nunavut: Implications for Archean Crustal Extraction and Cratonization." Geoscience Canada 42, no. 3 (2015): 305. http://dx.doi.org/10.12789/geocanj.2015.42.065.

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SUMMARYThe Repulse Bay block (RBb) of the southern Melville Peninsula, Nunavut, lies within the Rae craton and exposes a large (50,000 km2) area of middle to lower crust. The block is composed of ca. 2.86 Ga and 2.73–2.71 Ga tonalite-trondhjemite-granodiorite (TTG) and granitic gneiss that was derived from an older 3.25 and 3.10 Ga crustal substrate. This period of crustal generation was followed by the emplacement of ca. 2.69–2.66 Ga enderbite, charnockite, and granitoid intrusions with entrained websterite xenoliths. These voluminous batholith-scale bodies (dehydrated and hydrated intrusions

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7

Wei, Han, Li Ting-mei, Cheng Lu-lu, Liu Lu, Yu Lu-ji, and Peng Zhao-xu. "Effect of adding microorganism and carbon source to substrate on nitrogen removal treating the drainage of WWTP." Water Science and Technology 79, no. 10 (2019): 1947–55. http://dx.doi.org/10.2166/wst.2019.192.

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Abstract Developing substrate with carbon release properties is helpful to enhance nitrogen removal in low C/N ratio wastewater treatment. In this study, substrates with and without adding carbon source and microorganism were prepared to treat the drainage effluent from a wastewater treatment plant (WWTP), the difference in nitrogen removal were investigated. The results showed that adding a carbon source and microorganism to substrates could not only increase the amount of chemical oxygen demand (COD) released, but also enhance the adsorption capacities of NH4+-N and NO3–-N. The carbon releas

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8

Seviour, E. M., K. Eales, L. Izzard, M. Beer, E. L. Carr, and R. J. Seviour. "The in situ physiology of Nostocoida limicola II, a filamentous bacterial morphotype in bulking activated sludge, using fluorescence in situ hybridization and microautoradiography." Water Science and Technology 54, no. 1 (2006): 47–53. http://dx.doi.org/10.2166/wst.2006.370.

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The in situ physiology of the actinobacterial bulking and foaming filamentous bacterium “Nostocoida limicola” II was studied by fluorescence in situ hybridization/microautoradiography. Substrate assimilation patterns of pure cultures of this bacterium were different to those seen in activated sludge biomass samples. There was no evidence to suggest that “N. limicola” II preferred hydrophobic substrates, but evidence was produced to support the view that it is metabolically active under anaerobic conditions in activated sludge.

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9

Yamaguchi, Chisato, Soudthedlath Khamsalath, Yuki Takimoto, et al. "SLIM1 Transcription Factor Promotes Sulfate Uptake and Distribution to Shoot, Along with Phytochelatin Accumulation, Under Cadmium Stress in Arabidopsis thaliana." Plants 9, no. 2 (2020): 163. http://dx.doi.org/10.3390/plants9020163.

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Sulfur (S) assimilation, which is initiated by sulfate uptake, generates cysteine, the substrate for glutathione (GSH) and phytochelatin (PC) synthesis. GSH and PC contribute to cadmium (Cd) detoxification by capturing it for sequestration. Although Cd exposure is known to induce the expression of S-assimilating enzyme genes, including sulfate transporters (SULTRs), mechanisms of their transcriptional regulation are not well understood. Transcription factor SLIM1 controls transcriptional changes during S deficiency (−S) in Arabidopsis thaliana. We examined the potential involvement of SLIM1 in

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10

Pincus, D. H., D. C. Coleman, W. R. Pruitt, et al. "Rapid Identification of Candida dubliniensis with Commercial Yeast Identification Systems." Journal of Clinical Microbiology 37, no. 11 (1999): 3533–39. http://dx.doi.org/10.1128/jcm.37.11.3533-3539.1999.

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Candida dubliniensis is a newly described species that is closely related phylogenetically to Candida albicans and that is commonly associated with oral candidiasis in human immunodeficiency virus-positive patients. Several recent studies have attempted to elucidate phenotypic and genotypic characteristics of use in separating the two species. However, results obtained with simple phenotypic tests were too variable and tests that provided more definitive data were too complex for routine use in the clinical laboratory setting. The objective of this study was to determine if reproducible identi

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11

Aggelis, G. "Two alternative pathways for substrate assimilation byMucor circinelloides." Folia Microbiologica 41, no. 3 (1996): 254–56. http://dx.doi.org/10.1007/bf02814626.

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12

Dott, W., and P. Kämpfer. "Biochemical Methods for Automated Bacterial Identification and Testing Metabolic Activities in Water and Wastewater." Water Science and Technology 20, no. 11-12 (1988): 221–27. http://dx.doi.org/10.2166/wst.1988.0288.

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The improvements of computer-assisted identification were used to develop a new microplate system for characterization and identification of various gram-negative and gram-positive heterotrophic bacteria from the environment. In standard microtitration plates about 90 biochemical tests, some of them conventional tests, carbon substrate assimilation tests and enzyme tests using chromogenic substrates can be performed. Reading of the test results is done automatically by a photometer coupled to a computer. The applications of this identification system are shown by two waste water samples.

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13

Jankauskienė, Julė, Aušra Brazaitytė, Viktorija Vaštakaitė Kairienė, and Vytautas Zalatorius. "EFFECTS OF PEAT AND PEAT-ZEOLITE SUBSTRATES ON QUALITY, GROWTH INDICES OF CUCUMBER SEEDLINGS AND CROP PRODUCTIVITY." Acta Scientiarum Polonorum Hortorum Cultus 18, no. 5 (2019): 161–70. http://dx.doi.org/10.24326/asphc.2019.5.16.

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The objective of this study was to evaluate peat and zeolite mixtures substrates performance on cucumber seedling quality and crop yield. The research was carried out in a greenhouse covered with double polymeric film at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry. Cucumber seedlings were grown in different substrates: peat, peat + zeolite 1 : 1, peat + zeolite 2 : 1, peat + zeolite 3 : 1 and peat + zeolite 4 : 1. Cucumber seedlings grown in peat and zeolite substrates are shorter; the leaf area is smaller than that of the seedlings grown in peat alon

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14

Shimakawa, Ginga, Ayaka Kohara, and Chikahiro Miyake. "Characterization of Light-Enhanced Respiration in Cyanobacteria." International Journal of Molecular Sciences 22, no. 1 (2020): 342. http://dx.doi.org/10.3390/ijms22010342.

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In eukaryotic algae, respiratory O2 uptake is enhanced after illumination, which is called light-enhanced respiration (LER). It is likely stimulated by an increase in respiratory substrates produced during photosynthetic CO2 assimilation and function in keeping the metabolic and redox homeostasis in the light in eukaryotic cells, based on the interactions among the cytosol, chloroplasts, and mitochondria. Here, we first characterize LER in photosynthetic prokaryote cyanobacteria, in which respiration and photosynthesis share their metabolisms and electron transport chains in one cell. From the

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15

Kong, Y. H., M. Beer, R. J. Seviour, K. C. Lindrea, and G. A. Rees. "Role of “G-bacteria” in anaerobic substrate uptake in a SBR with no phosphorus removal." Water Science and Technology 46, no. 1-2 (2002): 171–78. http://dx.doi.org/10.2166/wst.2002.0473.

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Biomass from an SBR running with no enhanced biological phosphorus removal (EBPR) but which exhibited anaerobic assimilation of glucose and acetate, was dominated by “G-bacteria”, cocci in tetrads and clusters. Extracted 16S rDNA was amplified by PCR and then analysed using Denaturing Gradient Gel Electrophoresis (DGGE). Major bands were extracted and their sequences determined. Clone libraries were also prepared, the 16S rDNA extracted, PCR performed and the resultant fragments run by DGGE to aid in identifying the DGGE bands and provide fuller sequences than available by DGGE alone. The two

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16

Riou, V., S. Halary, S. Duperron, et al. "Influence of chemosynthetic substrates availability on symbiont densities, carbon assimilation and transfer in the dual symbiotic vent mussel <I>Bathymodiolus azoricus</I>." Biogeosciences Discussions 5, no. 3 (2008): 2279–304. http://dx.doi.org/10.5194/bgd-5-2279-2008.

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Abstract. High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was already proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 a

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17

Hesselsoe, Martin, Jeppe Lund Nielsen, Peter Roslev, and Per Halkjær Nielsen. "Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14CO2." Applied and Environmental Microbiology 71, no. 2 (2005): 646–55. http://dx.doi.org/10.1128/aem.71.2.646-655.2005.

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ABSTRACT Most heterotrophic bacteria assimilate CO2 in various carboxylation reactions during biosynthesis. In this study, assimilation of 14CO2 by heterotrophic bacteria was used for isotope labeling of active microorganisms in pure cultures and environmental samples. Labeled cells were visualized by microautoradiography (MAR) combined with fluorescence in situ hybridization (FISH) to obtain simultaneous information about activity and identity. Cultures of Escherichia coli and Pseudomonas putida assimilated sufficient 14CO2 during growth on various organic substrates to obtain positive MAR si

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18

Nogina, T. M., L. A. Khomenko, V. S. Pidgorskyi, and M. A. Kharkhota. "Fatty Acid Composition of Rhodococcus aetherivorans Cells During Phenol Assimilation." Mikrobiolohichnyi Zhurnal 83, no. 4 (2021): 15–23. http://dx.doi.org/10.15407/microbiolj83.04.015.

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The ability of microorganisms to survive in unfavorable conditions and maintain their biodegradation activity is mainly associated with changes in the composition of their cellular lipids. One of the factors of negative impact on cells can be their interaction with petroleum hydrocarbons, especially monoaromatic compounds, which are toxic to microorganisms even in small amounts. Aim. To research the changes in the cell fatty acids composition of Rhodococcus aetherivorans UCM Ac-602 strain during phenol degradation. Methods. The cultivation of the strain was carried out in a liquid mineral medi

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19

Chow, Virginia, Young Sik Kim, Mun Su Rhee та ін. "A 1,3-1,4-β-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2". Applied and Environmental Microbiology 82, № 6 (2016): 1789–98. http://dx.doi.org/10.1128/aem.03526-15.

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ABSTRACTPaenibacillussp. strain JDR-2 (PaenibacillusJDR-2) secretes a multimodular cell-associated glycoside hydrolase family 10 (GH10) endoxylanase (XynA10A1) that catalyzes the depolymerization of methylglucuronoxylan (MeGXn) and rapidly assimilates the products of depolymerization. Efficient utilization of MeGXnhas been postulated to result from the coupling of the processes of exocellular depolymerization and assimilation of oligosaccharide products, followed by intracellular metabolism. Growth and substrate utilization patterns with barley glucan and laminarin similar to those observed wi

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20

Rücker, Nadine, Sandra Billig, René Bücker, Dieter Jahn, Christoph Wittmann, and Franz-Christoph Bange. "Acetate Dissimilation and Assimilation in Mycobacterium tuberculosis Depend on Carbon Availability." Journal of Bacteriology 197, no. 19 (2015): 3182–90. http://dx.doi.org/10.1128/jb.00259-15.

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ABSTRACTMycobacterium tuberculosispersists inside granulomas in the human lung. Analysis of the metabolic composition of granulomas from guinea pigs revealed that one of the organic acids accumulating in the course of infection is acetate (B. S. Somashekar, A. G. Amin, C. D. Rithner, J. Troudt, R. Basaraba, A. Izzo, D. C. Crick, and D. Chatterjee, J Proteome Res 10:4186–4195, 2011, doi:http://dx.doi.org/10.1021/pr2003352), which might result either from metabolism of the pathogen or might be provided by the host itself. Our studies characterize a metabolic pathway by whichM. tuberculosisgenera

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21

Voss, Jörn, Armin Ehrenreich, and Wolfgang Liebl. "Characterization and inactivation of the membrane-bound polyol dehydrogenase in Gluconobacter oxydans DSM 7145 reveals a role in meso-erythritol oxidation." Microbiology 156, no. 6 (2010): 1890–99. http://dx.doi.org/10.1099/mic.0.037598-0.

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The growth of Gluconobacter oxydans DSM 7145 on meso-erythritol is characterized by two stages: in the first stage, meso-erythritol is oxidized almost stoichiometrically to l-erythrulose according to the Bertrand–Hudson rule. The second phase is distinguished from the first phase by a global metabolic change from membrane-bound meso-erythritol oxidation to l-erythrulose assimilation with concomitant accumulation of acetic acid. The membrane-associated erythritol-oxidizing enzyme was found to be encoded by a gene homologous to sldA known from other species of acetic acid bacteria. Disruption of

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22

Gargouri, Boutheina, Najla Mhiri, Fatma Karray, Fathi Aloui, and Sami Sayadi. "Isolation and Characterization of Hydrocarbon-Degrading Yeast Strains from Petroleum Contaminated Industrial Wastewater." BioMed Research International 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/929424.

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Two yeast strains are enriched and isolated from industrial refinery wastewater. These strains were observed for their ability to utilize several classes of petroleum hydrocarbons substrates, such asn-alkanes and aromatic hydrocarbons as a sole carbon source. Phylogenetic analysis based on the D1/D2 variable domain and the ITS-region sequences indicated that strains HC1 and HC4 were members of the generaCandidaandTrichosporon, respectively. The mechanism of hydrocarbon uptaking by yeast,Candida,andTrichosporonhas been studied by means of the kinetic analysis of hydrocarbons-degrading yeasts gr

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23

Herrmann, Elena, Wayne Young, Douglas Rosendale, et al. "RNA-Based Stable Isotope Probing SuggestsAllobaculumspp. as Particularly Active Glucose Assimilators in a Complex Murine Microbiota Cultured In Vitro." BioMed Research International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/1829685.

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RNA-based stable isotope probing (RNA-SIP) and metabolic profiling were used to detect actively glucose-consuming bacteria in a complex microbial community obtained from a murine model system. A faeces-derived microbiota was incubated under anaerobic conditions for 0, 2, and 4 h with 40 mM [U13C]glucose. Isopycnic density gradient ultracentrifugation and fractionation of isolated RNA into labeled and unlabeled fractions followed by 16S rRNA sequencing showed a quick adaptation of the bacterial community in response to the added sugar, which was dominated by unclassified Lachnospiraceae species

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24

Skelly, David K., and Jennifer Golon. "ASSIMILATION OF NATURAL BENTHIC SUBSTRATES BY TWO SPECIES OF TADPOLES." Herpetologica 59, no. 1 (2003): 37–42. http://dx.doi.org/10.1655/0018-0831(2003)059[0037:aonbsb]2.0.co;2.

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25

Kunz, Daniel A., Jui-Lin Chen та Guangliang Pan. "Accumulation of α-Keto Acids as Essential Components in Cyanide Assimilation by Pseudomonas fluorescens NCIMB 11764". Applied and Environmental Microbiology 64, № 11 (1998): 4452–59. http://dx.doi.org/10.1128/aem.64.11.4452-4459.1998.

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ABSTRACT Pyruvate (Pyr) and α-ketoglutarate (αKg) accumulated when cells of Pseudomonas fluorescens NCIMB 11764 were cultivated on growth-limiting amounts of ammonia or cyanide and were shown to be responsible for the nonenzymatic removal of cyanide from culture fluids as previously reported (J.-L. Chen and D. A. Kunz, FEMS Microbiol. Lett. 156:61–67, 1997). The accumulation of keto acids in the medium paralleled the increase in cyanide-removing activity, with maximal activity (760 μmol of cyanide removed min−1 ml of culture fluid−1) being recovered after 72 h of cultivation, at which time the

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26

Linkès, Marion, Marco Martins Afonso, Pascal Fede, Jérôme Morchain, and Philippe Schmitz. "Numerical study of substrate assimilation by a microorganism exposed to fluctuating concentration." Chemical Engineering Science 81 (October 2012): 8–19. http://dx.doi.org/10.1016/j.ces.2012.07.003.

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27

Ward, B. B. "Light and substrate concentration relationships with marine ammonium assimilation and oxidation rates." Marine Chemistry 16, no. 4 (1985): 301–16. http://dx.doi.org/10.1016/0304-4203(85)90052-0.

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28

Jezdinský, Aleš, and Vojtěch Řezníček. "The effect of some cultivation conditions on properties of leaves of the container-produced species Alnus glutinosa (L.) Gaertn." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 59, no. 3 (2011): 81–90. http://dx.doi.org/10.11118/actaun201159030081.

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Plants differ in their reactions to unfavourable environmental conditions and the effect of the acting factors directly and indirectly results in changes in a number of plants organs. The leaves are vegetative organs and as such they are completely exposed to external conditions. Most markedly these conditions affect the assimilative organs. The objective of the present study was to evaluate the effect of four substrates and two sites differing in light intensity on the leaf morphology of the model plant species Alnus glutinosa (L.) Gaertn. The used substrates were the commercial peat-bark sub

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29

Jang, Song Yee, Jungwon Hwang, Byoung Sik Kim, Eun-Young Lee, Byung-Ha Oh, and Myung Hee Kim. "Structural basis of inactivation of Ras and Rap1 small GTPases by Ras/Rap1-specific endopeptidase from the sepsis-causing pathogen Vibrio vulnificus." Journal of Biological Chemistry 293, no. 47 (2018): 18110–22. http://dx.doi.org/10.1074/jbc.ra118.004857.

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Multifunctional autoprocessing repeats-in-toxin (MARTX) toxins are secreted by Gram-negative bacteria and function as primary virulence-promoting macromolecules that deliver multiple cytopathic and cytotoxic effector domains into the host cytoplasm. Among these effectors, Ras/Rap1-specific endopeptidase (RRSP) catalyzes the sequence-specific cleavage of the Switch I region of the cellular substrates Ras and Rap1 that are crucial for host innate immune defenses during infection. To dissect the molecular basis underpinning RRSP-mediated substrate inactivation, we determined the crystal structure

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Riou, V., S. Halary, S. Duperron, et al. "Influence of CH<sub>4</sub> and H<sub>2</sub>S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel <i>Bathymodiolus azoricus</i>." Biogeosciences 5, no. 6 (2008): 1681–91. http://dx.doi.org/10.5194/bg-5-1681-2008.

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Abstract. High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between

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31

Evans, R. D., M. J. Bennett, and D. Hauton. "Perfused heart studies to investigate lipid metabolism." Biochemical Society Transactions 28, no. 2 (2000): 113–20. http://dx.doi.org/10.1042/bst0280113.

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The isolated perfused heart preparation is an invaluable model for investigating metabolism in a variety of physiological and pathological states. It avoids confounding systemic factors (e.g. endocrine, metabolic and work load changes) and permits simultaneous measurement of mechanical function. The ability to measure arteriovenous concentration differences across the myocardium and the coronary flow rate, together with the use of radiolabelled substrates, permits assessment of substrate assimilation and disposition of most potential energetic substrates. In the case of lipids, metabolism of n

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32

Celińska, Ewelina, Jean-Marc Nicaud, and Wojciech Białas. "Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin." Applied Microbiology and Biotechnology 105, no. 3 (2021): 975–89. http://dx.doi.org/10.1007/s00253-021-11097-1.

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Abstract Consolidated bioprocessing (CBP) featuring concomitant hydrolysis of renewable substrates and microbial conversion into value-added biomolecules is considered to bring substantial benefits to the overall process efficiency. The biggest challenge in developing an economically feasible CBP process is identification of bifunctional biocatalyst merging the ability to utilize the substrate and convert it to value-added product with high efficiency. Yarrowia lipolytica is known for its exceptional performance in hydrophobic substrates assimilation and storage. On the other hand, its capacit

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González-Villanueva, Miriam, Hemanshi Galaiya, Paul Staniland, et al. "Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol." International Journal of Molecular Sciences 20, no. 22 (2019): 5737. http://dx.doi.org/10.3390/ijms20225737.

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Cupriavidus necator H16 is a non-pathogenic Gram-negative betaproteobacterium that can utilize a broad range of renewable heterotrophic resources to produce chemicals ranging from polyhydroxybutyrate (biopolymer) to alcohols, alkanes, and alkenes. However, C. necator H16 utilizes carbon sources to different efficiency, for example its growth in glycerol is 11.4 times slower than a favorable substrate like gluconate. This work used adaptive laboratory evolution to enhance the glycerol assimilation in C. necator H16 and identified a variant (v6C6) that can co-utilize gluconate and glycerol. The

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García-Sánchez, Francisco, and James P. Syvertsen. "Substrate Type and Salinity Affect Growth Allocation, Tissue Ion Concentrations, and Physiological Responses of Carrizo Citrange Seedlings." HortScience 44, no. 5 (2009): 1432–37. http://dx.doi.org/10.21273/hortsci.44.5.1432.

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To gain insight into salinity tolerance of citrus, we studied growth, leaf, and root Cl– concentrations and physiological responses of 5-month-old seedlings of the citrus rootstock Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliate L.] grown in a greenhouse in three different substrates: Candler sand soil, Floridana sandy clay soil, or a commercial soilless peat/perlite/vermiculite potting media. Plants were kept well-watered with a complete nutrient solution plus either no salt (control) or 50 mM NaCl for 9 weeks. Without salinity, substrate type did not affect total plant grow

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Silva, Rebeca Fasioli, Maria do Socorro Mascarenhas Santos, Larissa Pires Mueller, Claudia Andrea Lima Cardoso, and Margareth Batistote. "The composition of sacarine substrates for ethanol production and the fermentative capacity Saccharomyces cerevisiae Pedra-2." Research, Society and Development 9, no. 11 (2020): e44891110235. http://dx.doi.org/10.33448/rsd-v9i11.10235.

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The production of ethanol in Brazil is based on sugarcane juice, however other biomasses can be used for this process, such as sweet sorghum. However, some nutrients can interfere with fermentation, such as the presence of metals, carbon and nitrogen sources, which can affect the fermentation capacity of yeasts. Thus, this study aims to analyze the presence of fundamental nutrients present in saccharine substrates, as well as their assimilation and conversion of ethanol by the yeast Pedra-2. Samples of sugarcane and sorghum juice were obtained, in which analysis of the presence of metals was c

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Gieseke, Armin, Jeppe Lund Nielsen, Rudolf Amann, Per Halkjaer Nielsen, and Dirk de Beer. "In situ substrate conversion and assimilation by nitrifying bacteria in a model biofilm." Environmental Microbiology 7, no. 9 (2005): 1392–404. http://dx.doi.org/10.1111/j.1462-2920.2005.00826.x.

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Burton, Euan, and Vincent J. J. Martin. "Proteomic analysis ofClostridium thermocellumATCC 27405 reveals the upregulation of an alternative transhydrogenase–malate pathway and nitrogen assimilation in cells grown on cellulose." Canadian Journal of Microbiology 58, no. 12 (2012): 1378–88. http://dx.doi.org/10.1139/cjm-2012-0412.

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Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the ability to directly convert cellulosic biomass into useful products such as ethanol and hydrogen. In this study, a quantitative comparative proteomic analysis of the organism was performed to identify proteins and biochemical pathways that are differentially utilized by the organism after growth on cellobiose or cellulose. The cytoplasmic and membrane proteomes of C. thermocellum grown on cellulose or cellobiose were quantitatively compared using a metabolic15N isotope labelling method in conjunction with nan

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Nakajima, F., N. Kamiko, and K. Yamamoto. "Organic wastewater treatment without greenhouse gas emission by photosynthetic bacteria." Water Science and Technology 35, no. 8 (1997): 285–91. http://dx.doi.org/10.2166/wst.1997.0324.

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High conversion of wastewater into biomass of photosynthetic bacteria was investigated. This biomass is possibly utilized as feed for fish or extraction source of PHA (polyhydroxyalkanoate). As a fundamental investigation, batch experiments were carried out using purple nonsulfur bacteria Rhodobacter sphaeroides to determine the growth rate and yield and to quantify CO2 release/uptake during substrate assimilation. The substrates used for this study were five kinds of VFA namely, acetate, propionate, n-butyrate, n-valeric acid and n-caproate. Growth rates for n-valeric acid and n-cvaproate wer

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Ruiz, Jose Luis, and Maria del Carmen Salas. "Evaluation of Organic Substrates and Microorganisms as Bio-Fertilisation Tool in Container Crop Production." Agronomy 9, no. 11 (2019): 705. http://dx.doi.org/10.3390/agronomy9110705.

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Microorganisms are only effective when adequate conditions for their survival and development are provided. Among the factors that influence its effectiveness includes the type of soil or culture substrate, which works as an energy source reserve. Therefore, a tomato and a melon crop were established in different cycles to assess the effect of the physicochemical properties of organic substrates based on coconut fibre and vermicompost in three proportions, 0:100, 40:60 and 60:40 (% v:v), on the microbial activity in the rhizosphere when the bacteria Azotobacter vinelandii, Bacillus megaterium

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Hudson, Benjamin H., Andrew T. Hale, Ryan P. Irving, Shenglan Li, and John D. York. "Modulation of intestinal sulfur assimilation metabolism regulates iron homeostasis." Proceedings of the National Academy of Sciences 115, no. 12 (2018): 3000–3005. http://dx.doi.org/10.1073/pnas.1715302115.

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Sulfur assimilation is an evolutionarily conserved pathway that plays an essential role in cellular and metabolic processes, including sulfation, amino acid biosynthesis, and organismal development. We report that loss of a key enzymatic component of the pathway, bisphosphate 3′-nucleotidase (Bpnt1), in mice, both whole animal and intestine-specific, leads to iron-deficiency anemia. Analysis of mutant enterocytes demonstrates that modulation of their substrate 3′-phosphoadenosine 5′-phosphate (PAP) influences levels of key iron homeostasis factors involved in dietary iron reduction, import and

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Schneider, Barbara L., and Lawrence J. Reitzer. "Salmonella typhimurium nit Is nadE: Defective Nitrogen Utilization and Ammonia-Dependent NAD Synthetase." Journal of Bacteriology 180, no. 17 (1998): 4739–41. http://dx.doi.org/10.1128/jb.180.17.4739-4741.1998.

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ABSTRACT S. typhimurium nit mutants are defective in nitrogen assimilation, despite having normal levels of assimilatory enzymes. Complementation, enzyme assays, and genetic mapping show thatnit is nadE. We present evidence that ammonia, not glutamine, is the physiological substrate for eubacterial NAD synthetases and that low activity completely accounts for the mutant phenotype.

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Meisl, Tomáš. "The evaluation of the growth and nutrition conditions of the garden nursery material Prunus and Thuja according to the use of various cultivating substrates and systems of fertilization." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 54, no. 1 (2006): 31–46. http://dx.doi.org/10.11118/actaun200654010031.

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The influence of different peat-based cultivating substrates and the system of fertilization on the nutrition conditions and growth characteristics of garden nursery material Prunus kurilensis ‘Brillant’ and Thuja occidentalis ‘Smaragd’ were observed during a three-year experiment. Three kinds of substrates were tested: peat + pumice (pemza) proportioned 8:2, fermented bark + peat + clay proportioned 4:4:2, fermented bark + peat + clay proportioned 4:4:2. Two fertilizers were used: granular controlled-release fertilizer – Osmocote, and watersoluble with irrigation – Kristalon.A higher content

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Nairn, Carol, Iván Rodríguez, Yolanda Segura, et al. "Alkalinity, and Not the Oxidation State of the Organic Substrate, Is the Key Factor in Domestic Wastewater Treatment by Mixed Cultures of Purple Phototrophic Bacteria." Resources 9, no. 7 (2020): 88. http://dx.doi.org/10.3390/resources9070088.

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Domestic wastewater treatment by purple phototrophic bacteria (PPB) is based on the assimilative uptake of organics and nutrients into the bacterial biomass. Thereby, it strongly depends on the carbon/nutrients ratio of the wastewater. The physiological COD/N/P ratio for PPB growth in domestic wastewater makes the addition of an external organic carbon source necessary in order to allow for an efficient process. However, PPB need a source of alkalinity (as CO2) to grow on reduced organics that serves as an electron acceptor since biohydrogen production (an alternative electron sink) is inhibit

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Malashenko, Yuri, Ivan Sokolov, and Victoria Romanovskaya. "Role of monooxygenase reaction during assimilation of non-growth substrates by methanotrophs." Journal of Molecular Catalysis B: Enzymatic 10, no. 1-3 (2000): 305–12. http://dx.doi.org/10.1016/s1381-1177(00)00131-4.

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Li, Xiaowen, Haitao Xia, Jinwang Wang, and Qiuxia Chen. "Nutrient uptake and assimilation in fragrant rosewood (Dalbergia odorifera T.C. Chen) seedlings in growing media with un-composted spent mushroom residue." PLOS ONE 16, no. 4 (2021): e0249534. http://dx.doi.org/10.1371/journal.pone.0249534.

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The purpose of this study was to detect nutrient uptake and assimilation in woody plants subjected to growing media with some peat replaced by spent mushroom residue (SMR). Fragrant rosewood (Dalbergia odorifera T.C. Chen) seedlings were cultured in five types of growing media with SMR and peat in volumetric proportions of 0% (control), 25%, 50%, 75%, and 100%. With the increase of SMR proportion, ammonium- and nitrate-nitrogen (N) concentrations declined but available phosphorus (P) concentration and electrical conductance both increased. Seedlings in the full SMR substrates showed obvious mo

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Moreno, Renata, and Fernando Rojo. "The Target for the Pseudomonas putida Crc Global Regulator in the Benzoate Degradation Pathway Is the BenR Transcriptional Regulator." Journal of Bacteriology 190, no. 5 (2007): 1539–45. http://dx.doi.org/10.1128/jb.01604-07.

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ABSTRACT Crc protein is a global regulator involved in catabolite repression control of several pathways for the assimilation of carbon sources in pseudomonads when other preferred substrates are present. In Pseudomonas putida cells growing exponentially in a complete medium containing benzoate, Crc strongly inhibits the expression of the benzoate degradation genes. These genes are organized into several transcriptional units. We show that Crc directly inhibits the expression of the peripheral genes that transform benzoate into catechol (the ben genes) but that its effect on genes correspondin

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Truax, Benoit, Daniel Gagnon, France Lambert, and Normand Chevrier. "Nitrate assimilation of raspberry and pin cherry in a recent clearcut." Canadian Journal of Botany 72, no. 9 (1994): 1343–48. http://dx.doi.org/10.1139/b94-164.

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An experimental study conducted in a recently clear-cut hardwood forest was used to determine the reduction of nitrate in the leaves of Rubus idaeus L. and Prunus pensylvanica L.F., early successional species and shrub competitors of forest tree regeneration. A factorial experimental design was chosen and the main factors were the shrub competitors and the replicates (blocks). The nitrate reductase enzyme assay was chosen as a physiological marker since it is substrate induced and it is the first key enzyme implicated in the assimilation of nitrate. Results show that for 2 consecutive years, p

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Bonch-Osmolovskaya, Elizaveta A., Anna A. Perevalova, Tatiana V. Kolganova, Igor I. Rusanov, Christian Jeanthon, and Nikolay V. Pimenov. "Activity and Distribution of Thermophilic Prokaryotes in Hydrothermal Fluid, Sulfidic Structures, and Sheaths of Alvinellids (East Pacific Rise, 13°N)." Applied and Environmental Microbiology 77, no. 8 (2011): 2803–6. http://dx.doi.org/10.1128/aem.02266-10.

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ABSTRACTProcesses of inorganic carbon assimilation, methanogenesis, sulfate reduction, and acetate oxidation to CO2occurring in samples from the East Pacific Rise at 13°N were traced, using radioisotopically labeled substrates, at temperatures ranging from 65 to 100°C. Molecular hydrogen stimulated lithotrophic methanogenesis and sulfate reduction but inhibited inorganic carbon assimilation. Active mineralization of acetate was observed in an organic-richAlvinella-associated system at 80°C. Members of theThermococcaleswere the most numerous hyperthermophilic archaea in these samples, their den

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VELASCO-GARCÍA, Roberto, Lilian GONZÁLEZ-SEGURA, and Rosario A. MUÑOZ-CLARES. "Steady-state kinetic mechanism of the NADP+- and NAD+-dependent reactions catalysed by betaine aldehyde dehydrogenase from Pseudomonas aeruginosa." Biochemical Journal 352, no. 3 (2000): 675–83. http://dx.doi.org/10.1042/bj3520675.

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Betaine aldehyde dehydrogenase (BADH) catalyses the irreversible oxidation of betaine aldehyde to glycine betaine with the concomitant reduction of NAD(P)+ to NADP(H). In Pseudomonas aeruginosa this reaction is a compulsory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors. The kinetic mechanisms of the NAD+- and NADP+-dependent reactions were examined by steady-state kinetic methods and by dinucleotide binding experiments. The double-reciprocal patterns obtained for initial velocity with NAD(P)+ and for product and dead-end inhibition e

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Mohn, Marie, Besarta Thaqi, and Katrin Fischer-Schrader. "Isoform-Specific NO Synthesis by Arabidopsis thaliana Nitrate Reductase." Plants 8, no. 3 (2019): 67. http://dx.doi.org/10.3390/plants8030067.

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Nitrate reductase (NR) is important for higher land plants, as it catalyzes the rate-limiting step in the nitrate assimilation pathway, the two-electron reduction of nitrate to nitrite. Furthermore, it is considered to be a major enzymatic source of the important signaling molecule nitric oxide (NO), that is produced in a one-electron reduction of nitrite. Like many other plants, the model plant Arabidopsis thaliana expresses two isoforms of NR (NIA1 and NIA2). Up to now, only NIA2 has been the focus of detailed biochemical studies, while NIA1 awaits biochemical characterization. In this study

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