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1

Kline, Ahnika, Harry Porterfield, and A. Zelazny. "661. Futility of Bacterial Bone Marrow Cultures: Experience over a 19 Year Period." Open Forum Infectious Diseases 7, Supplement_1 (2020): S386. http://dx.doi.org/10.1093/ofid/ofaa439.854.

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Abstract Background Bone marrow biopsies are often performed on patients with unclear diagnoses and cultures may be ordered for both routine bacterial, mycobacterial and fungal pathogens. They are performed in semi-sterile conditions and involve needle penetration through the skin, posing an increased risk of skin contamination. These cultures also require a substantial amount of laboratory personnel time. Methods Cultures collected from 2001-2020 were surveyed in the lab electronic record. We assessed the culture type (fungal, bacterial, mycobacterial), and the presence of pathogens and conta
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A.M., Jana, and Singh Pragya. "Bacterial Preservation." International Journal of Life Sciences and Technology (IJLST) ISSN:0974-5335 13, no. 1 (2020): 1–29. https://doi.org/10.5281/zenodo.4014767.

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Bacterial Preservation A.M. Jana<sup>1</sup> and Pragya Singh<sup>2</sup> 1 Director (R&amp;D) &amp; Department of Biotechnology, Vijayaraje Institute of Science and Management (VISM), Gwalior-474001, M.P., INDIA 2. Principal and Director VISM Group of Studies &amp; Department of Biotechnology, Vijayaraje Institute of Science and Management (VISM), Gwalior-474001, M.P., INDIA amjana@rediffmail.com, pragyasingh797@gmail.com, vism_gwalior@rediffmail.com <strong>ABSTRACT</strong>: The present work pertains to preservation of bacterial culture using liquid nutrient media incorporated with a simple
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O’Boyle, C. J., J. MacFie, C. J. Mitchell, D. Johnstone, P. M. Sagar, and P. C. Sedman. "Microbiology of bacterial translocation in humans." Gut 42, no. 1 (1998): 29–35. http://dx.doi.org/10.1136/gut.42.1.29.

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Background—Gut translocation of bacteria has been shown in both animal and human studies. Evidence from animal studies that links bacterial translocation to the development of postoperative sepsis and multiple organ failure has yet to be confirmed in humans.Aims—To examine the spectrum of bacteria involved in translocation in surgical patients undergoing laparotomy and to determine the relation between nodal migration of bacteria and the development of postoperative septic complications.Methods—Mesenteric lymph nodes (MLN), serosal scrapings, and peripheral blood from 448 surgical patients und
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Rochayani, Masithoh Yessi, Dahlia Gladiola Rurina Menufandu, and Rahmila Dapa. "Investigating the Growth of Bacteria using Double Sigmoid Model with Reparameterization." International Journal of Global Optimization and Its Application 2, no. 4 (2023): 200–208. http://dx.doi.org/10.56225/ijgoia.v2i4.239.

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The growth of an organism can be modeled using a growth curve. However, bacteria's growth pattern differs from other organisms. Bacterial growth is divided into four phases: lag, logarithmic, stationary, and death. The experts re-parameterized the growth curve to match the growth phase of the bacteria. Bacterial growth patterns generally do not show a single sigmoid pattern but form two curves. Therefore, the double sigmoid model is more suitable. This study modeled the growth of the Pseudomonas putida bacteria by observing the optical density of the medium. Model parameters are estimated usin
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Sharma, Meeta, and Meena Metre. "Bacterial vaginosis: impact of treatment and associated risk factors: a cross section study." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 13, no. 1 (2023): 72–79. http://dx.doi.org/10.18203/2320-1770.ijrcog20234081.

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Background: Bacterial vaginosis (BV) is a condition caused by an overgrowth of normal vaginal flora. Many socio-demographic factors are associated with bacterial vaginosis. Antibiotic resistance to some organism is a challenge in treatment of bacterial vaginosis. Methods: This cross-sectional study was conducted including 100 women with reproductive age group presenting with complain of vaginal discharge. After clinical examination, vaginal swab was collected of patients who gave consent for same. Socio-demographic characteristics were recorded and risk factors were assessed. The slide smears
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Salim, Muhammad Umer, Syed Mohammad Umair Dilawar, and Syed Tabish Rehman. "Frequency of Bacteriological Organism in Patients with Thoracic Empyema." Pakistan Journal of Medical and Health Sciences 15, no. 6 (2021): 1914–16. http://dx.doi.org/10.53350/pjmhs211561914.

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Objective: To examine the frequency of bacteriological organism present in pleural fluid, in patients positive with thoracic empyema, assessed on the basis of pus culture test. Study Design: Cross-sectional study Place and Duration: Inpatient Department, Chest Medicine Ward 12, JPMC, Karachi, Pakistan for six months duration from 11 February 2019 to 10 August 2019. Methodology: One hundred and thirty nine patients diagnosed with Thoracic Empyema were included in this study. All patients included in the study shall undergo Pus Culture Test prior to identify the presence or absence of bacterial
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7

Ichsanto Permadi, Adeh Mahardika, and Misriyani. "Case Report: A 52-year-old female with Bacterial Infection and Drug Allergy." World Journal of Advanced Research and Reviews 25, no. 30 (2025): 238–51. https://doi.org/10.30574/wjarr.2025.25.3.0679.

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This bacterial infection has a major impact on public health. There are many bacteria that are able to adapt to survive in water, soil, food, and various other places. The development of bacterial infection to cause disease is influenced by several factors including the infectivity of the organism, the pathogenicity of infectious organisms, the ability of pathogenic bacteria to avoid the body's immunity, virulence factors of pathogenic bacteria, and the role of human immunity cells themselves. In the treatment of patients with bacterial infections there are some real challenges in the use of d
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Amany, Eltayib Ataelmanan, Dawoud Abakar Adam, and Mohammed Hamdan Elsadig. "Isolation and identification of the causative agent of bacterial throat infection according to a response to commonly antibiotic." GSC Biological and Pharmaceutical Sciences 12, no. 1 (2020): 267–72. https://doi.org/10.5281/zenodo.4271125.

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Bacterial throat infection has a worldwide distribution, affecting persons of all ages, races, social strata and both genders. The present study was a prospective cross-sectional study aimed to isolate and identify the bacteria which cause throat infection and their antibiotic susceptibility in Madani &ndash; Sudan. Throat swabs were collected from 100 patients attended to Ear, Nose and Throat (ENT) Teaching Hospital. Isolation and identification of bacteria were made by directly inoculated onto blood agar, Gram stain and biochemical test. All isolated organisms were tested for their in vitro
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Miah, M. S., P. Nix, A. Koukkoullis, and J. Sandoe. "Microbial causes of complicated acute bacterial rhinosinusitis and implications for empirical antimicrobial therapy." Journal of Laryngology & Otology 130, no. 2 (2015): 169–75. http://dx.doi.org/10.1017/s0022215115003369.

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AbstractObjectives:Very little up-to-date information is available on the microbiology of complicated acute bacterial rhinosinusitis. Reliable microbiology testing is necessary to inform current empirical antimicrobial treatments. This study was conducted in response to recent US guidelines on antimicrobial treatments for acute bacterial rhinosinusitis. It aimed to describe the pathological micro-organisms involved in complicated acute bacterial rhinosinusitis in the UK and their antimicrobial susceptibility.Methods:This study collected retrospective data on cases of complicated acute bacteria
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Calcagnile, Matteo, Salvatore Maurizio Tredici, Adelfia Talà, and Pietro Alifano. "Bacterial Semiochemicals and Transkingdom Interactions with Insects and Plants." Insects 10, no. 12 (2019): 441. http://dx.doi.org/10.3390/insects10120441.

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A peculiar feature of all living beings is their capability to communicate. With the discovery of the quorum sensing phenomenon in bioluminescent bacteria in the late 1960s, it became clear that intraspecies and interspecies communications and social behaviors also occur in simple microorganisms such as bacteria. However, at that time, it was difficult to imagine how such small organisms—invisible to the naked eye—could influence the behavior and wellbeing of the larger, more complex and visible organisms they colonize. Now that we know this information, the challenge is to identify the myriad
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Rasheed, Farhan, Iqra Jamil, Rai Gull Fraz, Aisha Sultan, Muhammad Bilal, and Jawad Niaz Khan. "SKIN, SOFT TISSUE INFECTIONS AND THEIR MICROBIOLOGICAL PROFILE FROM A TERTIARY CARE HOSPITAL." Pakistan Postgraduate Medical Journal 35, no. 01 (2024): 7–11. http://dx.doi.org/10.51642/ppmj.v35i01.638.

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Background: Skin and soft tissue infections (SSTIs) are considered as diverse group of infections which may vary in microbial etiology and clinical presentation and ranges from simple, uncomplicated infections to severe, complicated picture. Causative organisms with antimicrobial resistance may worsen the situation.&#x0D; Objectives: The aim of present study was to check the frequency and antibiotic sensitivity pattern of bacterial isolates causing SSTIs.&#x0D; Methods: A retrospective study performed over a period of 3 years. Pus and wound samples were collected and were processed by using st
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Toyyibah, Ilma Dzurriyyatan, Musofa Rusli, and Juniastuti Juniastuti. "BACTERIAL PATTERN AMONG SEPSIS PATIENTS IN INTERNAL MEDICINE INPATIENT WARD DR. SOETOMO GENERAL ACADEMIC HOSPITAL, SURABAYA, INDONESIA IN 2017-2019." Majalah Biomorfologi 32, no. 2 (2022): 52–58. http://dx.doi.org/10.20473/mbiom.v32i1.2022.52-58.

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Highlights:1. Bacteria remain a major cause of bacterial sepsis.2. The most common causal agent in bacterial septicaemia was the gram-positive bacterium. Abstract: Background: Bacteria remain the primary cause of bacterial sepsis. Gram-negative bacteria are the most commonly isolated from sepsis patients. However, gram-positive bacterial infections have also increased recently. Objective: To identify the pattern of bacterial infection in sepsis patients in Internal Medicine inpatient ward Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Material and Method: This retrospective study
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Patil, Swati V., and Roshan R. Mane. "Bacterial and clinical profile of diabetic foot ulcer using optimal culture techniques." International Journal of Research in Medical Sciences 5, no. 2 (2017): 496. http://dx.doi.org/10.18203/2320-6012.ijrms20170139.

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Background: Diabetic foot ulcers (DFU) are the complications of diabetes mellitus. The diabetic foot ulcer infections are polymicrobial in nature. If they are not recognized and controlled it leads to many devastating consequences like limb amputation, sepsis, and even mortality. Hence, the present study was undertaken to determine the bacterial and clinical profile of diabetic foot ulcer using optimal culture techniques and the antimicrobial sensitivity pattern of the isolates.Methods: A total number of 103 patients with a foot ulcer of Wagner’s grade II or more and evidence of purulent exuda
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Ahmed, Omar B., and Elsadig M. Hamdan. "Profile of Bacterial Conjunctivitis in Sudan." Scholars Journal of Applied Medical Sciences 4, no. 4 (2016): 1217–21. http://dx.doi.org/10.36347/sjams.2016.v04i04.023.

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Bacterial conjunctivitis has worldwide distribution, affecting persons of all ages, races, social strataand both genders. The present study aimed to isolate and identify the common bacteria causing conjunctivitis and their antibiotic susceptibility in Khartoum state, Sudan. Conjunctival swabs were collected from 200 patients attended to Khartoum Eye Hospital and allwaldin Eye Hospital. The swabs were directly inoculated onto blood agar, MacConkey agar and chocolate agar then a smear was prepared from each swab. For C. trachomatis identification was based on Giemsa stain to the air dried smeara
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Tomasinsig, Linda, Marco Scocchi, Romina Mettulio, and Margherita Zanetti. "Genome-Wide Transcriptional Profiling of the Escherichia coli Response to a Proline-Rich Antimicrobial Peptide." Antimicrobial Agents and Chemotherapy 48, no. 9 (2004): 3260–67. http://dx.doi.org/10.1128/aac.48.9.3260-3267.2004.

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ABSTRACT Most antimicrobial peptides (AMPs) impair the viability of target bacteria by permeabilizing bacterial membranes. However, the proline-rich AMPs have been shown to kill susceptible organisms without causing significant membrane perturbation and may act by inhibiting the activity of bacterial targets. To gain initial insight into the events that follow interaction of a proline-rich peptide with bacterial cells, we used DNA macroarray technology to monitor transcriptional alterations of Escherichia coli in response to challenge with a subinhibitory concentration of the proline-rich Bac7
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16

MacFie, J., C. O’Boyle, C. J. Mitchell, P. M. Buckley, D. Johnstone, and P. Sudworth. "Gut origin of sepsis: a prospective study investigating associations between bacterial translocation, gastric microflora, and septic morbidity." Gut 45, no. 2 (1999): 223–28. http://dx.doi.org/10.1136/gut.45.2.223.

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AIMSTo investigate the “gut origin of sepsis” hypothesis.METHODSProspective controlled study of 279 surgical patients in which cultures of nasogastric aspirates were compared with those obtained from mesenteric lymph nodes taken at laparotomy and the organisms cultured from subsequent septic complications. Bacterial translocation was confirmed if positive cultures were obtained from mesenteric lymph nodes. Postoperative sepsis was defined as any positive culture in the postoperative period. Bacterial species obtained in gastric microflora, mesenteric lymph nodes, and postoperative septic compl
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17

Wang, Weiying, and Shu Hua Li. "Use of common blood parameters for the differential diagnosis of childhood infections." PLOS ONE 17, no. 9 (2022): e0273236. http://dx.doi.org/10.1371/journal.pone.0273236.

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Background Routine laboratory investigations are not rapidly available to assist clinicians in the diagnosis of pediatric acute infections. Our objective was to evaluate some common blood parameters and use them for the differential diagnosis of childhood infections. Methods This retrospective study was conducted between October 2019 and September 2020 at Guangzhou Women and Children’s Medical Center, China. We performed blood tests in patients infected with DNA viruses (n = 402), RNA viruses (n = 602), gram-positive organisms (G+; n = 421), gram-negative organisms (G−; n = 613), or Mycoplasma
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18

Rose, L. J., and E. W. Rice. "Inactivation of bacterial biothreat agents in water, a review." Journal of Water and Health 12, no. 4 (2014): 618–33. http://dx.doi.org/10.2166/wh.2014.038.

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Water supplies and water distribution systems have been identified as potential targets for contamination by bacterial biothreat agents. Since the 2001 Bacillus anthracis bioterrorist attacks, additional efforts have been aimed at research to characterize biothreat organisms in regards to their susceptibility to disinfectants and technologies currently in use for potable water. Here, we present a review of research relevant to disinfection of bacteria with the potential to pose a severe threat to public health and safety, and their potential surrogates. The efficacy of chlorine, monochloramine
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van Hougenhouck-Tulleken, Wesley G., Pedro H. Lebre, Mohamed Said, and Don A. Cowan. "Bacterial pathogens in peritoneal dialysis peritonitis: Insights from next-generation sequencing." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 40, no. 6 (2020): 581–86. http://dx.doi.org/10.1177/0896860820908473.

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Background: Peritoneal dialysis (PD) peritonitis is a feared complication of PD, with significant sequelae for the patient. The cause of PD peritonitis is largely due to a single organism (≥75% of cases) and rarely due to multiple organisms. Methods: In this pilot study, we investigated 25 cases of PD peritonitis with 16S ribosomal RNA (rRNA) next-generation sequencing (NGS) techniques. Results: Total concordance between culture and NGS was noted. In addition, the NGS technique was highly sensitive, identifying 33 different bacteria (including a nonculturable bacterium), compared to 13 bacteri
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Boskabadi, Hassan, Maryam Zakerihamidi, Fatemeh Bagheri, and Ali Taghipour. "Bacterial Microorganisms in Definitive Neonatal Sepsis." Journal of Guilan University of Medical Sciences 32, no. 2 (2023): 96–105. http://dx.doi.org/10.32598/jgums.32.2.593.2.

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Background The proper diagnosis and treatment of neonatal sepsis is done depends on the understanding of its causative organisms. Objective The present study aimed to investigate the types of microorganisms in definitive neonatal sepsis. Methods Neonatal sepsis is a serious infectious disease with high mortality. And its diagnosed by blood culture. It includes early (appearance of clinical symptoms from birth to the third-seventh day) and late (from the third day to the twenty-eighth day) types with specific and sometimes common clinical symptoms. This cross-sectional study was performed on 26
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Whiting, Michael S., W. M. Ingledew, Sun Y. Lee, and Barry Ziola. "Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci." Canadian Journal of Microbiology 45, no. 8 (1999): 670–77. http://dx.doi.org/10.1139/w99-060.

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Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surfa
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Chunduri, Jayaprada Rao, Prarthana Singh P, Arati P Nair, Nidhi A Patel, and Sonali B Mehta. "Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study." Biosciences Biotechnology Research Asia 20, no. 1 (2023): 211–21. http://dx.doi.org/10.13005/bbra/3082.

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ABSTRACT: Microbes present in the digestive tract of an organism are of great importance. The microbial qualitative and quantitative composition represents the physical, physiological conditions, habits, habitats of the organisms as well as their association patterns. The microbes of digestive system have prominent role not only in the sustainability of the organism but also in the food processing industry. The marine cephalopods are one of the preferred marine food resources, next to marine fisheries. The current study aims to understand the microbial content in the digestive system of consum
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Potselueva, L. A. "Binase as a medicinal agent." Kazan medical journal 75, no. 3 (1994): 172–74. http://dx.doi.org/10.17816/kazmj89772.

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Mondal, Montosh Kumar, Beauty Rani Roy, Sabina Yeasmeen, Faizul Haque, AK Qumrul Huda, and Debabrata Banik. "Prevalence of microorganism and emergence of bacterial resistance in ICU of Bangabandhu Sheikh Mujib Medical University of Bangladesh." Journal of the Bangladesh Society of Anaesthesiologists 26, no. 1 (2014): 20–26. http://dx.doi.org/10.3329/jbsa.v26i1.19811.

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Background Antibiotic resistant bacterial nosocomial infections are a leading problem in intensive care units (ICU). Objective To study the pattern of microorganism and bacterial resistant to antibiotic in ICU of Bangabandhu sheikh Mujib Medical University of Bangladesh. Methods This retrospective study was conducted in ICU of Bangabandhu Sheikh Mujib Medical University, Bangladesh from January 2010 to December 2012. Total number of samples were 448. The samples of tracheal aspirate, blood and urine for culture and sensitivity was collected from the patient admitted in ICU. Analysis of trachea
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Shimada, Tomohiro, and Hideji Yoshida. "Overview of the Molecular Mechanism of Bacterial Environmental Adaptation by Comprehensive Analysis." International Journal of Molecular Sciences 24, no. 8 (2023): 7602. http://dx.doi.org/10.3390/ijms24087602.

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Kalaitzidou, Maria P., Maria V. Alvanou, Konstantinos V. Papageorgiou, et al. "Pollution Indicators and HAB-Associated Halophilic Bacteria Alongside Harmful Cyanobacteria in the Largest Mussel Cultivation Area in Greece." International Journal of Environmental Research and Public Health 19, no. 9 (2022): 5285. http://dx.doi.org/10.3390/ijerph19095285.

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Taking into consideration the essential contribution of Mytilus galloprovincialis farming, it is of rising importance to add knowledge regarding bacterial species occurrence in water samples from aquaculture zones from the point of view of both the organism and public health. In the present study, we investigated the bacterial community existing in water samples from six Mytilus galloprovincialis aquaculture areas in the Thermaikos gulf, northern Greece, that may provoke toxicity in aquatic organisms and humans and may indicate environmental pollution in mussel production as well as algal bloo
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Kalaitzidou, Maria P., Maria V. Alvanou, Konstantinos V. Papageorgiou, et al. "Pollution Indicators and HAB-Associated Halophilic Bacteria Alongside Harmful Cyanobacteria in the Largest Mussel Cultivation Area in Greece." International Journal of Environmental Research and Public Health 19, no. 9 (2022): 5285. http://dx.doi.org/10.3390/ijerph19095285.

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Taking into consideration the essential contribution of Mytilus galloprovincialis farming, it is of rising importance to add knowledge regarding bacterial species occurrence in water samples from aquaculture zones from the point of view of both the organism and public health. In the present study, we investigated the bacterial community existing in water samples from six Mytilus galloprovincialis aquaculture areas in the Thermaikos gulf, northern Greece, that may provoke toxicity in aquatic organisms and humans and may indicate environmental pollution in mussel production as well as algal bloo
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Ofori-Darko, Ernest, Yana Zavros, Gabriele Rieder, Susan A. Tarlé, Mary Van Antwerp, and Juanita L. Merchant. "An OmpA-Like Protein from Acinetobacterspp. Stimulates Gastrin and Interleukin-8 Promoters." Infection and Immunity 68, no. 6 (2000): 3657–66. http://dx.doi.org/10.1128/iai.68.6.3657-3666.2000.

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ABSTRACT Bacterial overgrowth in the stomach may occur under conditions of diminished or absent acid secretion. Under these conditions, secretion of the hormone gastrin is elevated. Alternatively, bacterial factors may directly stimulate gastrin. Consistent with this hypothesis, we found that mice colonized for 2 months with a mixed bacterial culture of opportunistic pathogens showed an increase in serum gastrin. To examine regulation of gene expression by bacterial proteins, stable transformants of AGS cells expressing gastrin or interleukin-8 (IL-8) promoters were cocultured with live organi
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Moore, John E., Mark McCalmont, Jiru Xu, B. Cherie Millar, and Neville Heaney. "Asaia sp., an Unusual Spoilage Organism of Fruit-Flavored Bottled Water." Applied and Environmental Microbiology 68, no. 8 (2002): 4130–31. http://dx.doi.org/10.1128/aem.68.8.4130-4131.2002.

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ABSTRACT A gram-negative bacillus was isolated from a batch of fruit-flavored bottled water, which had spoiled as a result of bacterial overgrowth (&gt;106 CFU/ml). The spoilage organism was extremely difficult to identify phenotypically and was poorly identified as Pasturella sp. (78.7% identification profile) employing the API 20NE identification scheme, which gave the profile 5040000. Molecular identification through PCR amplification of a partial region of the 16S rRNA gene followed by direct automated sequencing of the PCR amplicon allowed identification of the organism. Due to the sequen
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Marma, Chusung Ching, Shakeel Ahmed, Farjana Akhter, Shamim Ara Keya, Parash Ullah, and Shrabanti Barua. "Detection of Biofilm Producing Uropathogenic Bacteria and Their Antibiotic Sensitivity Pattern." Scholars Journal of Applied Medical Sciences 10, no. 8 (2022): 1244–51. http://dx.doi.org/10.36347/sjams.2022.v10i08.013.

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Background: Urinary tract infection is one the most common infection in clinical practice. Uropathogens have the ability to form biofilm in urinary tract, frequently within the indwelling catheter. Microorganism growing in a biofilm is associated with chronic and recurrent UTI and less sensitive to antimicrobial agent. So, the aim of the present study was to detect biofilm-producing uropathogenic bacteria by microtiter plate assay and antibiotic sensitivity pattern of biofilm- producing and biofilm non-producing organisms. Methods: This cross- sectional observational study was carried out in M
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Burkhart, Craig N., and Craig G. Burkhart. "Bacterial Symbiotes, Their Presence in Head Lice, and Potential Treatment Avenues." Journal of Cutaneous Medicine and Surgery 10, no. 1 (2006): 2–6. http://dx.doi.org/10.1007/7140.2006.00003.

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Background: Pediculus humanus capitis (head lice) belongs to the order Anoplura, which are blood-feeding ectoparasites that live on human hair. Within these insects reside symbiotic bacteria that enable the insect to flourish on dietary sources of limited nutritional value. These symbiotic bacteria are essential to the survival of the insect. Objective: To assess the feasibility of treating head lice by altering their symbiotic bacteria. Methods: In addition to a literature review of the expanded role of symbiotic bacteria in other organisms, the anatomic localization of their presence in huma
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Krzyżewska, Eva, and Jacek Rybka. "Biosynthesis of lipopolysaccharides with different length of the O-specific region as a virulence factor of Gram-negative bacteria." Postępy Higieny i Medycyny Doświadczalnej 72 (July 6, 2018): 573–86. http://dx.doi.org/10.5604/01.3001.0012.1735.

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The outer membrane of Gram-negative bacteria is a biological structure with a unique composition that significantly contributes to the survival of bacteria in the unfavorable conditions of the host organism. The lipopolysaccharide constitutes about 70% of the external part of the outer membrane. The LPS molecule is composed of three different parts: lipid A, core oligosaccharide and O antigen. Despite the O-specific antigen being one of the most intensely studied surface structures of bacterial polysaccharides, a number of questions regarding the mechanism of the O antigen biosynthesis and its
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Silvester, Alexander, Timothy Neal, Gabriela Czanner, Michael Briggs, Simon Harding, and Stephen Kaye. "Adult bacterial conjunctivitis: resistance patterns over 12 years in patients attending a large primary eye care centre in the UK." BMJ Open Ophthalmology 1, no. 1 (2016): e000006. http://dx.doi.org/10.1136/bmjophth-2016-000006.

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PurposeTo determine whether there was a change in the resistance pattern of bacteria isolated from cases of conjunctivitis following the introduction of over-the-counter availability of chloramphenicol in 2005.Design and settingRetrospective review of laboratory records for adult patients with suspected bacterial conjunctivitis between 2001 and 2012 attending the Royal Liverpool University Hospital.ParticipantsPatients with suspected bacterial conjunctivitis. Organisms were identified by standard laboratory methods. Scanty growth and normal flora were considered as a negative result. For posit
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Kuznetsova, D. A., V. A. Rykova, and O. N. Podladchikova. "Bacterial Siderophores: Structure, Functions, and Role in the Pathogenesis of Infections." Problems of Particularly Dangerous Infections, no. 3 (October 29, 2022): 14–22. http://dx.doi.org/10.21055/0370-1069-2022-3-14-22.

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This review systematizes and analyzes the data published over the past decade, devoted to the study of low-molecular-weight high affinity iron chelators – siderophores. Siderophores, which are found in bacteria, fungi and mammals, are able to extract iron from insoluble inorganic compounds, and in the host organism – from complexes with proteins that perform the function of nonspecific protection of mammals from infections. The extracted iron is delivered to cells through surface protein receptors specific for each siderophore, as well as various protein transport systems that make up membrane
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A.G., Aghayeva. "Creation of a bacterial library based on strains isolated from soil of Azerbaijan and their screening for the presence of new antibacterial biomolecules." Journal of Life Sciences and Biomedicine 74, no. 2 (2019): 13–19. https://doi.org/10.5281/zenodo.7391193.

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The major goal of the research project carried out during the years 2014 - 2018 was to establish a library of microorganisms, including novel, previously uncultured bacterial species, isolated from soil of Azerbaijan, and their antibacterial screening. For this, 30 soil samples were collected from different zones of Azerbaijan and sent to the Fraunhofer USA Center for Molecular Biotechnology for bacterial isolation and screening. 578 bacterial strains were isolated from 30 soil samples and a new bacterial library was created. All 578 isolated strains were analyzed for antibacterial activity ag
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Shrestha, S., NC Shrestha, S. Dongol Singh, et al. "Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU." Kathmandu University Medical Journal 11, no. 1 (2014): 66–70. http://dx.doi.org/10.3126/kumj.v11i1.11030.

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Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were cultu
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Luque-Almagro, Víctor M., Andrew J. Gates, Conrado Moreno-Vivián, Stuart J. Ferguson, David J. Richardson, and M. Dolores Roldán. "Bacterial nitrate assimilation: gene distribution and regulation." Biochemical Society Transactions 39, no. 6 (2011): 1838–43. http://dx.doi.org/10.1042/bst20110688.

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In the context of the global nitrogen cycle, the importance of inorganic nitrate for the nutrition and growth of marine and freshwater autotrophic phytoplankton has long been recognized. In contrast, the utilization of nitrate by heterotrophic bacteria has historically received less attention because the primary role of these organisms has classically been considered to be the decomposition and mineralization of dissolved and particulate organic nitrogen. In the pre-genome sequence era, it was known that some, but not all, heterotrophic bacteria were capable of growth on nitrate as a sole nitr
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Purushottam M, Deborah, and Nesepogu Padmaja. "Bacterial isolates and their biofilm formation on contact lenses." IP International Journal of Medical Microbiology and Tropical Diseases 8, no. 1 (2022): 10–14. http://dx.doi.org/10.18231/j.ijmmtd.2022.003.

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Using contact lenses without proper care and sterilization measures can lead to eye infection like keratitis and other eye diseases. About 25-30% of cases with corneal ulcers are related to contacts lens use. Microbial agents after their entry, they further invade into corneal layers by their virulence factors and enzymatics secreted by the bacteria. Wearing contact lenses also increase stress on cornea so the cornea has less ability to defend against the bacteria which is invading. Some of the bacteria are also capable of biofilm formation. Biofilms formed on contact lens surfaces has increas
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Dekio, Itaru, Mitsuo Sakamoto, Hidenori Hayashi, Masayuki Amagai, Makoto Suematsu, and Yoshimi Benno. "Characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16S rRNA gene-based comprehensive analysis." Journal of Medical Microbiology 56, no. 12 (2007): 1675–83. http://dx.doi.org/10.1099/jmm.0.47268-0.

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A previous study using bacterial 16S rRNA gene-based clone libraries revealed that the microbiota in healthy human skin included uncultured micro-organisms, although the micro-organisms in skin exposed to disease conditions remain to be examined. To compare the profiles of skin microbiota in 13 patients with atopic dermatitis (AD) and 10 healthy controls, terminal RFLP analysis of bacterial 16S rRNA genes was applied to 23 swab-scrubbed samples from facial skin. This culture-independent analysis successfully revealed the complex bacterial members of the microbiota as peak patterns following ca
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Thapa, Neeraj, Bhairav Kumar Hamal, Nagendra Prashad Yadav, and Ghanashyam Thapa. "Bacterial Translocation as a Cause of Postoperative Sepsis in Surgical Patients Undergoing Laparotomy." Journal of Lumbini Medical College 2, no. 2 (2014): 28. http://dx.doi.org/10.22502/jlmc.v2i2.53.

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Introduction: Bacterial translocation is the invasion of indigenous intestinal bacteria through the gut mucosa to normally sterile tissues and the internal organs. Objective of the study is to examine the spectrum of bacteria involved in translocation in surgical patients undergoing laparotomy and to determine the relation between nodal migration of bacteria and the development of postoperative septic complications.&#x0D; Methods: Mesenteric lymph nodes (MLN)culture was done in patients undergoing elective and emergency surgeries fulfilling the inclusion criteria.&#x0D; Results:Bacterial trans
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Villalba, Maria I., Leonardo Venturelli, Ronnie Willaert, et al. "Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence." Microorganisms 9, no. 8 (2021): 1545. http://dx.doi.org/10.3390/microorganisms9081545.

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Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantilever and in monitoring its nanometric scale oscillations as a function of different physical-chemical stimuli. Up to now, we only used the cantilever oscillations variance signal to assess the viability of the attached organisms. In this contribution, we demonstrate that a more precise analysis of the motion pattern of the cantilever can unvei
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Horseman, Tim, Carmen Campbell, Grace Gomes, and Keith Fong. "Using Electric Cell-Substrate Impedance Sensing (ECIS) as a novel method to evaluate bacterial urinary tract infections." Journal of Immunology 204, no. 1_Supplement (2020): 82.16. http://dx.doi.org/10.4049/jimmunol.204.supp.82.16.

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Abstract Urinary tractinfections (UTIs) are commonly acquired bacterial infections that cause significant morbidity, worsened by the rise of antimicrobial resistance among uropathogenic bacteria. Understanding the dynamics of bacterial UTIs is essential to the proper use of antimicrobial therapies in patient treatment. In this study, Electric Cell-Substrate Impedance Sensing (ECIS) technology was used to monitor bacterial-mediated cytotoxicity in-vitro enabling evaluation of antibiotics. Using the ECIS system, real-time changes of barrier function were recorded for human bladder epithelial cel
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Hansen, Anlaug Ådland, Solveig Langsrud, Ingunn Berget, Mari Øvrum Gaarder, and Birgitte Moen. "High Oxygen Packaging of Atlantic Cod Fillets Inhibits Known Spoilage Organisms, but Sensory Quality Is Not Improved Due to the Growth of Carnobacterium/Carnobacteriaceae." Foods 10, no. 8 (2021): 1754. http://dx.doi.org/10.3390/foods10081754.

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Improved quality control and prolonged shelf life are important actions in preventing food waste. To get an overview of the bacterial diversity of fillets from live stored mature Atlantic cod, bacterial isolates were identified before and after storage (air and vacuum) and freezing/thawing. Based on the load of dominating bacteria, the effect of different packaging methods and a short freezing/thawing process on prolonged shelf-life was evaluated (total viable counts, bacteriota, sensory attributes, and volatile components). Hand filleted (strict hygiene) cod fillets had a low initial bacteria
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Khare, J. "Microbiological Profile of Diabetic foot Ulcers Experience from a Tertiary care center in South India." International Journal of General Medicine & Surgery 1, no. 2 (2017): 1–5. https://doi.org/10.5281/zenodo.2639156.

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Aim: To determine the microbiological profile and antibiotic susceptibility pattern of organisms in diabetic foot ulcers in our population. Settings and Design: A prospective observational study Material and Method: A prospective study was carried out on 62 patients of diabetic foot ulcers attending the endocrine OPD. The aerobic bacterial agents were isolated, and their antibiotic susceptibility pattern was studied. Results: Organisms were cultured from 90.32% cases of diabetic foot ulcers. Staphylococcus aureus (both MRSA and non-MRSA) was the commonest organism found in 36.77% cases followe
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Zhang, Huan, Srutha Venkatesan, and Beiyan Nan. "Myxococcus xanthus as a Model Organism for Peptidoglycan Assembly and Bacterial Morphogenesis." Microorganisms 9, no. 5 (2021): 916. http://dx.doi.org/10.3390/microorganisms9050916.

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A fundamental question in biology is how cell shapes are genetically encoded and enzymatically generated. Prevalent shapes among walled bacteria include spheres and rods. These shapes are chiefly determined by the peptidoglycan (PG) cell wall. Bacterial division results in two daughter cells, whose shapes are predetermined by the mother. This makes it difficult to explore the origin of cell shapes in healthy bacteria. In this review, we argue that the Gram-negative bacterium Myxococcus xanthus is an ideal model for understanding PG assembly and bacterial morphogenesis, because it forms rods an
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Rela, Mariam, Sophia Opel, Sarah Williams, et al. "Operating Room Fomites as Potential Sources for Microbial Transmission in Burns Theatres." European Burn Journal 2, no. 1 (2021): 1–8. http://dx.doi.org/10.3390/ebj2010001.

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Background: Burn patients are susceptible to healthcare-associated infections. Contaminated surfaces play a role in microbial transmission. This study aimed to quantify the degree of contamination of burns theatre fomites during routine clinical use. Methods: The Patslide Patient Transfer Board (PAT slide) and operating table were investigated using two methods—bacterial swabs to culture viable organisms and adenosine triphosphate (ATP) swabs to measure biological material. Both items were sampled four times a day: before the first case, immediately after a case, immediately before the next ca
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Ranjan, Amit, and Anna Dongari-Bagtzoglou. "Tipping the Balance: C. albicans Adaptation in Polymicrobial Environments." Journal of Fungi 4, no. 3 (2018): 112. http://dx.doi.org/10.3390/jof4030112.

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Candida albicans is a pleiomorphic fungus which co-exists with commensal bacteria in mucosal and skin sites of mammalian hosts. It is also a major co-isolated organism from polymicrobial systemic infections, with high potential for morbidity or mortality in immunocompromised patients. Traditionally, resident mucosal bacteria have been thought to antagonize C. albicans in its ability to colonize or cause infection. However, recent investigations have revealed synergistic relationships with certain bacterial species that colonize the same mucosal sites with C. albicans. Such relationships broade
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Salipante, Stephen J., Toana Kawashima, Christopher Rosenthal, et al. "Performance Comparison of Illumina and Ion Torrent Next-Generation Sequencing Platforms for 16S rRNA-Based Bacterial Community Profiling." Applied and Environmental Microbiology 80, no. 24 (2014): 7583–91. http://dx.doi.org/10.1128/aem.02206-14.

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ABSTRACTHigh-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common “benchtop” sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16S rRNA (V1-V2) amplicon sequencing. We benchmarked performance by using a 20-organism mock bacterial community and a collection of primary human specimens. We observed comparatively higher error rates with the Ion Torrent platform and report a pattern of premature sequence
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Colomina, Beatriz, and Mark Wigley. "The Bacterial Clients of Modern Architecture." Cure and Care, no. 62 (2020): 6–17. http://dx.doi.org/10.52200/62.a.ysgg9kku.

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The human is an unstable idea; simultaneously an all-powerful creature – capable of transforming the whole ecology of the planet – yet extremely fragile, a murky ghost. Contemporary research into our microbiome portrays the human itself as a mobile ecology constructed by the endless flux of interactions between thousands of different species of bacteria – some of which are millions of years old and others joined us just a few months ago. This challenges conventional understandings of architecture. What does it mean to house the human when we no longer think that the human organism is securely
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Northrup, Justin D., Robert H. Mach, and Mark A. Sellmyer. "Radiochemical Approaches to Imaging Bacterial Infections: Intracellular versus Extracellular Targets." International Journal of Molecular Sciences 20, no. 22 (2019): 5808. http://dx.doi.org/10.3390/ijms20225808.

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The discovery of penicillin began the age of antibiotics, which was a turning point in human healthcare. However, to this day, microbial infections are still a concern throughout the world, and the rise of multidrug-resistant organisms is an increasing challenge. To combat this threat, diagnostic imaging tools could be used to verify the causative organism and curb inappropriate use of antimicrobial drugs. Nuclear imaging offers the sensitivity needed to detect small numbers of bacteria in situ. Among nuclear imaging tools, radiolabeled antibiotics traditionally have lacked the sensitivity or
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