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1

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, and Snehasis Jana. "Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone." Advances in Bioscience and Bioengineering 3, no. 6 (2015): 59–66. https://doi.org/10.11648/j.abb.20150306.12.

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Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray
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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, and Snehasis Jana. "Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone." Advances in Bioscience and Bioengineering 3, no. 6 (2015): 59–66. https://doi.org/10.5281/zenodo.192430.

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Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray
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Nagime, Pooja Vilas, Apichat Upaichit, Benjamas Cheirsilp, and Piyarat Boonsawang. "Bio-Succinic Acid Production from Palm Oil Mill Effluent Using Enterococcus gallinarum with Sequential Purification of Biogas." Fermentation 9, no. 4 (2023): 369. http://dx.doi.org/10.3390/fermentation9040369.

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Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered as a cheap substrate to lower the cost of production. It was revealed that 2-fold diluted POME produced more succinic acid than undiluted and 5-fold diluted POME. In addition, the effects of various neutralizing agents on succinic acid production utilized to manage pH and CO2 supply indicated that the utilization of MgCO3 as a neutralizing agent produced succinic acid of 11.5 g/L with a small amount of by-product synthesis. Plackett–Burman
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Thanigaivelan, V., T. K. Kishore Kumar, G. Hari Prasad, K. Daniel Jeysingh, and R. Mohit. "Extraction of Bio-oil from Sugarcane Leaves." Journal of Physics: Conference Series 2054, no. 1 (2021): 012010. http://dx.doi.org/10.1088/1742-6596/2054/1/012010.

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Abstract Bio oil production has become the main substitute for fossil-based fuels, but it should be available as a cost-effective method for its production. Extraction of bio-oil from sugarcane leaf is the motive of this project. We are going to achieve this by grinding the leaves freshly into a liquid form with Sugarcane Juicer that is going to be inoculated with Saccharomyces Cerevisiae (Brewer’s Yeast). The yeast culture was maintained in YPD (Yeast Peptone Dextrose) agar medium for 72 Hours. Once this Yeast grows, it is coagulated with the Sugarcane leaf extract that separates Bioethanol b
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Kumar Trivedi, Mahendra. "Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone." Advances in Bioscience and Bioengineering 3, no. 6 (2015): 59. http://dx.doi.org/10.11648/j.abb.20150306.12.

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6

Tomas, Ana F., Dimitar Karakashev, and Irini Angelidaki. "Effect of xylose and nutrients concentration on ethanol production by a newly isolated extreme thermophilic bacterium." Water Science and Technology 64, no. 2 (2011): 341–47. http://dx.doi.org/10.2166/wst.2011.730.

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An extreme thermophilic ethanol-producing strain was isolated from an ethanol high-yielding mixed culture, originally isolated from a hydrogen producing reactor operated at 70 °C. Ethanol yields were assessed with increasing concentrations of xylose, up to 20 g/l. The ability of the strain to grow without nutrient addition (yeast extract, peptone and vitamins) was also assessed. The maximum ethanol yield achieved was 1.28 molethanol/molxylose consumed (77% of the theoretical yield), at 2 g/l of initial xylose concentration. The isolate was able to grow and produce ethanol as the main fermentat
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7

Ghoddusi, Hamid B., and Richard K. Robinson. "Enumeration of starter cultures in fermented milks." Journal of Dairy Research 63, no. 1 (1996): 151–58. http://dx.doi.org/10.1017/s0022029900031629.

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SummarySome media available for the isolation and enumeration of starter cultures employed for the manufacture of cheese, yogurt and bio-yogurt were examined. Reddy's medium or a modification of Elliker's medium was found to be most satisfactory forLactococcusspp., while trypticase phytone yeast (TPY) agar with a mixture of antibiotics proved suitable for the discrete enumeration ofBifidobacteriumspp. The inclusion of Prussian blue (PB) in reinforced clostridial medium or tryptone proteose peptone yeast extract (TPPY) agar gave excellent differential counts for the starter bacteria in yogurt e
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Ovsyankina, Sofya, Sergey Khizhnyak, Polina Abolenceva, Yana Smol'nikova, and Elena Oleynikova. "RESISTANCE OF POTENTIAL AMYLASE PRODUCERS ALTERNARIA TENUISSIMA AND GEOMYCES PANNORUM TO LYOPHILIZATION." Bulletin of KSAU, no. 2 (December 24, 2024): 247–54. https://doi.org/10.36718/1819-4036-2023-2-247-254.

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The purpose of the study is to test the possibility of lyophilization of Alternaria tenuissima and Geomyces pannorum strains isolated by the authors as potential amylase producers for the food industry. The conidia of the studied strains were suspended in protective media and lyophilized using a Bio-Rus-4SFD lyophilizer. Three protective media were used: standard gelatin-sucrose agar (sucrose 10 %, gelatin 1.5 %, agar 0.01 %) recommended by the All-Russian Collection of Microorganisms; gelatin-sucrose agar with the addition of 1 % ascorbic acid as an antioxidant; peptone-sucrose-glycerol mixtu
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Ovsyankina, Sofya, Sergey Khizhnyak, Polina Abolenceva, Yana Smol'nikova, and Elena Oleynikova. "A NEW LIOPROTECTOR MEDIUM FOR LYOPHILIZATION OF NON-SYMBIOTIC NITROGEN-FIXING BACTERIA PROMISING FOR THE BIOFERTILIZERS PRODUCTION." Bulletin of KSAU, no. 12 (January 27, 2025): 54–61. https://doi.org/10.36718/1819-4036-2022-12-54-61.

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The aim of the study was to develop a protective medium for lyophilization of a strain of non-symbiotic nitrogen-fixing bacteria isolated by the authors from agricultural soils of the Krasnoyarsk Region and identified as Azotobacter sp. The strain was incubated on Sabouraud's modified nutrient medium at 25 °C for 7 days. After incubation, bacterial cells were suspended in protective media and lyophilized using a Bio-Rus-4SFD lyophilizer. Three protective media were used: standard gelatin-sucrose agar (sucrose 10 %, gelatin 1.5, agar 0.01 %), recommended by the All-Russian Collection of Microor
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10

Tsunatu, DY, KG Atiku, TT Samuel, BI Hamidu, and DI Dahutu. "PRODUCTION OF BIOETHANOL FROM RICE STRAW USING YEAST EXTRACTS PEPTONE DEXTROSE." Nigerian Journal of Technology 36, no. 1 (2016): 296–301. http://dx.doi.org/10.4314/njt.v36i1.36.

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The production of bio-ethanol from Rice Straw (Oryza sativa) was carried out using rice straw as a feedstock and a combination of Yeast Extracts Peptone Dextrose (YEPD)at 0.2%(w/v) 0.4%(w/v), 0.6%(w/v), 0.8%(w/v) and 1%(w/v) concentrations and Saccharomyces cerevisiae (yeast) at 0.5% (w/v), 1%(w/v), 1.5%(w/v), 2%(w/v) and 2.5%(w/v) concentrations as cells for fermentation. The study determined the most suitable pre-treatment method from the following pretreatment methods; 1M NaOH and heating. IM NaOH pre-treatment gave the highest cellulose and lowest lignin content. The effects of substrate c
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11

Saini, Meenaxi, Anu, Alexander Rapoport, et al. "Bioacetoin Production by Bacillus subtilis subsp. subtilis Using Enzymatic Hydrolysate of Lignocellulosic Biomass." Fermentation 9, no. 8 (2023): 698. http://dx.doi.org/10.3390/fermentation9080698.

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Acetoin is an important bio-product useful in the chemical, food and pharmaceutical industries. Microbial fermentation is the major process for the production of bioacetoin, as the petroleum resources used in chemical methods are depleting day by day. Bioacetoin production using wild microorganisms is an easy, eco-friendly and economical method for the production of bioacetoin. In the present study, culture conditions and nutritional requirements were optimized for bioacetoin production by a wild and non-pathogenic strain of B. subtilis subsp. subtilis JJBS250. The bacterial culture produced m
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Bhumbla, Upasana, and Amit Gupta. "A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan." International Journal Of Community Medicine And Public Health 8, no. 12 (2021): 5968. http://dx.doi.org/10.18203/2394-6040.ijcmph20214598.

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Background: Systemic candidiasis is associated with a high crude mortality rate, even with first line antifungal therapy. C. albicans is the predominant cause of invasive fungal diseases which is a serious public health issue. The main objective was to assess the reliability of different media for germ tube production in Candida albicans isolated from various clinically diagnosed pulmonary samples.Methods: All Candida isolates were identified and speciated by conventional methods such as Gram’s staining, germ tube test, chlamydospore formation on corn meal agar, sugar fermentation test, sugar
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Gorlas, Aurore, Karine Alain, Nadège Bienvenu, and Claire Geslin. "Thermococcus prieurii sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent." International Journal of Systematic and Evolutionary Microbiology 63, Pt_8 (2013): 2920–26. http://dx.doi.org/10.1099/ijs.0.026419-0.

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A novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2T, was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the ‘Sarah Spring’ area (7° 25′ 24″ S 107° 47′ 66″ W). Cells were irregular, motile cocci (0.8–1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2T grew at salt concentrations of 1–5 % (w/v) NaCl with an optimum at 2 %. The novel isola
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Andrey, Koshchaev Oksana Takhumova Ruslan Omarov Sergei Shlykov Nina Konik. "BIOTECHNOLOGY CULTIVATING THE PHYSIOLOGICALLY ADAPTED LACTOBACILLI TO CREATE MICROBIAL BIO-ADDITIVE FOR POULTRY." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 03 (2019): 5735–40. https://doi.org/10.5281/zenodo.2597500.

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<em>This article presents the results of research on the selection of the most suitable nutrient medium, ensuring maximum biomass growth of lactic acid microorganisms. The objects of research were pure cultures of microflora of the gastrointestinal tract of quails - Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. For the cultivation of microorganisms used 4 nutrient medium: medium for lactic acid microorganisms (Uglich), molasses autolysate medium (MAM), glucose-peptone medium (GPM) and medium MRS. In the cultivation of bacteria, the time and temperature of growth
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15

Nasrin, Tamanna, Ananda Kumar Saha, Moni Krishno Mohanta, Abu Reza, and Md Fazlul Haque. "Effect of Various Carbon and Nitrogen Sources on Decolorization of Textile Dyes by Pseudomonas taiwanensis Strain TNZ3." Journal of Advances in Microbiology 23, no. 2 (2023): 27–42. http://dx.doi.org/10.9734/jamb/2023/v23i2707.

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The co-substrate is a crucial component in the bio-decolorization process that helps bacteria to grow and carry out their fundamental functions. Different types of textile dyes with diverse chemical structures are frequently employed in the textile industry, and the most of them are resistant to biodegradation because of their xenobiotic nature. Due to the low carbon content of textile dyes, particularly of azo dyes, co-metabolic conditions are required for decolorization process. Hence, the current study was aimed to determine which striking carbon and nitrogen sources are preferred for gener
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Mor, Nitu, Prabhjot Kaur, Daljeet Kaur, Jitender Sharma, and Sunita Dalal. "Isolation of silicate solubilising microbes (SSM) from soil and water samples as potential components of bio fertilizers." Research Journal of Chemistry and Environment 28, no. 7 (2024): 94–100. http://dx.doi.org/10.25303/287rjce0940100.

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Silicate solubilizing microbes (SSM) are crop beneficial microbes which facilitate silica uptake via plant roots by way of dissolving silicates and making it available in the form of monomeric silicic acid. Excessive use of synthetic fertilizers may negatively affect overall soil health and the environment. To ensure that crops provide competitive yields while preserving the ecological balance of the soil, it is necessary to develop and use bio-fertilizers. For this, a consortium is required which can solubilise complex form of phosphates, silicates etc. to provide important nutrients to crops
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Jonuškienė, Ilona, Erika Davicijonaitė, Monika Vaškevičiūtė, et al. "Sustainable Production and Antioxidant Activity of Bacterial Xanthan Gum." Molecules 30, no. 13 (2025): 2734. https://doi.org/10.3390/molecules30132734.

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One of the world’s most sustainable solutions is to replace fossil-based polymers with biopolymers. The production of xanthan gum can be optimized using various renewable and cost-effective raw materials, which is a key focus in industrial biotechnology. Xanthan gum is a bioengineered thickening, stabilizing, and emulsifying agent. It has unique properties for use in many industries (food, biotechnology, petrochemicals, agricultural, cosmetics, wastewater treatment) and medical applications. It is tasteless, environmentally safe, non-toxic, and biodegradable. The biotechnological production of
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18

MRI, Mallik, Sikder MM, Hossain MK, Billah MB, and Alam N. "Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper." International Journal of Agricultural Research, Innovation and Technology 11, no. 2 (2021): 108–16. https://doi.org/10.3329/ijarit.v11i2.57263.

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Fruit rot disease of sweet pepper is one of the main fungal diseases causing huge economic losses to the grower. An experiment was conducted to find out the fungal pathogen associated with fruit rot disease of sweet pepper, obtained from experimental fields of Jahangirnagar University, Bangladesh. Fruit rot disease-causing fungus was isolated from infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well as molecular characterization based on internal transcribe spacer (ITS) region of the fungus. ITS sequence of our studied fungus MH
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Mallik, MRI, MM Sikder, MK Hossain, MB Billah, and N. Alam. "Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper." International Journal of Agricultural Research, Innovation and Technology 11, no. 2 (2022): 108–16. http://dx.doi.org/10.3329/ijarit.v11i2.57263.

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Fruit rot disease of sweet pepper is one of the main fungal diseases causing huge economic losses to the grower. An experiment was conducted to find out the fungal pathogen associated with fruit rot disease of sweet pepper, obtained from experimental fields of Jahangirnagar University, Bangladesh. Fruit rot disease-causing fungus was isolated from infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well as molecular characterization based on internal transcribe spacer (ITS) region of the fungus. ITS sequence of our studied fungus MH
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20

Grigs, Oskars, Elina Didrihsone, and Emils Bolmanis. "Investigation of a Broad-Bean Based Low-Cost Medium Formulation for Bacillus subtilis MSCL 897 Spore Production." Fermentation 9, no. 4 (2023): 390. http://dx.doi.org/10.3390/fermentation9040390.

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Bacillus subtilis (Bs) is a bacterium that benefits plants and is used in the production of bio-fungicides. The cultivation of Bs is a crucial step in bio-control preparation production, as it greatly impacts the quality and price of the final product. In a series of shake flask experiments, we investigated the economically feasible broth composition for spore production of Bacillus subtilis MSCL 897, a Latvian soil isolate. Our study investigated the impact of utilizing legume-based flours (such as broad bean, grey pea, and soybean) as the primary nitrogen source, along with sugar-beet molass
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Patel, Jitu, and Arvind Bhagwat. "Rapid real-time PCR assay for detecting Salmonella in raw and ready-to-eat meats." Acta Veterinaria Hungarica 56, no. 4 (2008): 451–58. http://dx.doi.org/10.1556/avet.56.2008.4.3.

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A real-time PCR assay was evaluated for the rapid detection (10 h) ofSalmonellain meats using molecular beacon probes available as a commercial kit (iQ-Check, Bio-Rad laboratories). Raw (chicken, pork) and ready-to-eat (RTE) meats were artificially contaminated withSalmonella entericaserovar Typhimurium at the estimated level of 2 to 4 cells per 25 g. After 8 h of pre-enrichment in buffered peptone water, a molecular beacon-based PCR assay was performed to detect contamination in raw and RTE meats. The sensitivity and accuracy of the assay were compared with the conventional USDA microbiologic
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22

Kumar Maharana, Abhas, and Shiv Mohan Singh. "Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica." Biosciences, Biotechnology Research Asia 15, no. 1 (2018): 05–16. http://dx.doi.org/10.13005/bbra/2603.

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Cold active amylase was investigated by bacteria and yeast isolates from the sediment core samples of Nella Lake,Larsemann Hills region, East Antarctica. Between potential yeast and bacteria isolates screened for amylases, best isolates were identified asRhodotorula sp. Y-37 and ArthrobacteralpinusN16 by molecular technique.Amylase production capabilities of both the isolate subjected for optimization processes by using submerged fermentation technique with soluble starch as substrate.The results indicate that a supplement of 1% w/v glucose, 1% w/v yeast extract and 0.1% w/v KCl at pH 7.0with
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23

Darwesh, Osama M., Mohamed R. Badr, Mousa A. Salem, Mohamed B. Atta, and Mahmoud E. El-sayed. "Production of biopigment by Monascus ruber AUMC 245 under submerged fermentation and its application as colorants of some food products." Natural Resources Conservation and Research 6, no. 2 (2023): 2418. http://dx.doi.org/10.24294/nrcr.v6i2.2418.

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The goal of the current investigation was to examine the production of red biopigment by Monascus ruber AUMC 245 during submerged fermentation and assess its safety as a bio-colorant in the food sector due to due to only sensory evaluation in food products. The various factors used for the production of red pigments, citrinin, and biomass yield were follows: 20 ℃–40 ℃ of temperature, 4.5–8.5 of pH, carbon and nitrogen sources. The optimum conditions were 30 ℃, pH 6.5, rice, peptone and incubation time for 10 days. The produced biopegment was without mycotoxin (citrinin). So, it is suitable for
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24

Rahman, MM, M. Salah Uddin, S. Zaman, et al. "Comparative study on growth and morphological characteristics of a wild type strain Rhizobium spp. (RCA-220) and a genetically engineered E. coli BL21." Journal of Bio-Science 20 (January 18, 2014): 75–82. http://dx.doi.org/10.3329/jbs.v20i0.17718.

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Context: Comparison between a wild type strain Rhizobium spp. (RCA-220) and a genetically modified strain E. coli BL21 in context of growth features. Objective: To observe the comparative growth characteristics of a genetically modified E. coli BL21 and an isolated wild type strain Rhizobium spp. (RCA-220). Materials and Methods: Different kinds of investigations were accomplished in both Luria-Bertani (LB) liquid and semi-solid media to observe the growth and maintenance of these strains. For the isolation of Rhizobium spp. selective Yeast Extract Manitol Agar (YEMA) was used. Colony morpholo
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MB, Billah, Sikder MM, Mallik MRI, Hossain MK, and Alam N. "Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures." International Journal of Agricultural Research, Innovation and Technology 11, no. 2 (2021): 124–32. https://doi.org/10.3329/ijarit.v11i2.57265.

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Present studies were conducted to isolate and identify the seed-borne pathogenic fungus from the selected tomato variety through morphological and molecular techniques based on the sequencing of internal transcribed spacer (ITS) region of 18S rDNA. According to the colony and conidial features, the fungus was identified as&nbsp;<em>Curvularia&nbsp;</em>sp. The obtained ITS sequencing showed above 99% similarity with&nbsp;<em>Curvularia lunata&nbsp;</em>in the NCBI database. The sequence of the fungus was deposited in NCBI GenBank under the accession number: ITS, MH382879.1. Besides, the phylog
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Paudyal, Som Prasad, Birendra Kunwar, Niroj Paudel, and Bishnu Dev Das. "Isolation and characterization of rhizobia from the root nodule of some cultivated legume crops." European Journal of Biological Research 11, no. 3 (2021): 294–306. https://doi.org/10.5281/zenodo.4906255.

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Biological nitrogen fixation (BNF) as a result of mutual symbiosis between the rhizobia and the cultivated legume have a vital role to balance the nutrient paucity in the soil. Modern researches shows that the BNF can be the important factors regulating and maintaining the defendable agriculture and ensuring food security especially in the developing countries. The use of artificial nitrogen fertilizers to rise crop yield is an internment farming practice, despite its unfavorable effects and hazards to the environment and human population which can be substituted by rhizobial inoculants as a b
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Rathour, Ranju K., Vaishali Sharma, Nidhi Rana, Ravi K. Bhatia, and Arvind K. Bhatt. "Bioremediation of Simulated Textile Effluent by an Efficient Bio-catalyst Purified from a Novel Pseudomonas fluorescence LiP-RL5." Current Chemical Biology 14, no. 2 (2020): 128–39. http://dx.doi.org/10.2174/2212796814666200406100247.

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Background: Microbial degradation of highly stable textile dyes, using lignin peroxidase, is an eco-friendly, less expensive and much advantageous in comparison to the chemical method. Objective: Biodegradation potential of lignin peroxidase (LiP), from Pseudomonas fluorescens LiP-RL5, was enhanced after optimization and purification so as to use it as a potential bioresource for the treatment of textile effluent. Methods: LiP producing bacterial isolate was primarily screened by methylene blue assay followed by LiP assay. The standard protocol was used for purification of lignin peroxidase an
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Arjuna, Anania, and Ekta Chitkara. "Bioproduction of Docosahexaenoic Acid by the Marine protist Schizochytrium." South Asian Journal of Experimental Biology 4, no. 6 (2015): 283–89. http://dx.doi.org/10.38150/sajeb.4(6).p283-289.

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The heterotrophic protist Schizochytrium sp. was batch cultivated in media containing glucose, yeast extract, peptone, dihydrogen potassium phosphate and sea salt prepared in distilled water. The increasing amounts of yeast ex-tract stimulated growth however, influenced lipid accumulation negatively. It has been observed that the sea salt concentrations above half the average seawater salinity were required for good growth and lipid accumulation. Schizochytrium sp. was able to grow on a glucose concentration as high as 60 g/l, which resulted in reduced biomass and lipid yield. Although, the cu
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Christwardana, Marcelinus, Linda Aliffia Yoshi, and J. Joelianingsih. "Energy Harvesting from Sugarcane Bagasse Juice using Yeast Microbial Fuel Cell Technology." Reaktor 21, no. 2 (2021): 52–58. http://dx.doi.org/10.14710/reaktor.21.2.52-58.

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This study demonstrates the feasibility of producing bioelectricity utilizing yeast microbial fuel cell (MFC) technology with sugarcane bagasse juice as a substrate. Yeast Saccharomyces cerevisiae was employed as a bio-catalyst in the production of electrical energy. Sugarcane bagasse juice can be used as a substrate in MFC yeast because of its relatively high sugar content. When yeast was used as a biocatalyst, and Yeast Extract, Peptone, D-Glucose (YPD) Medium was used as a substrate in the MFC in the acclimatization process, current density increased over time to reach 171.43 mA/m2 in close
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Krull, Susan, Silvia Brock, Ulf Prüße, and Anja Kuenz. "Hydrolyzed Agricultural Residues—Low-Cost Nutrient Sources for l-Lactic Acid Production." Fermentation 6, no. 4 (2020): 97. http://dx.doi.org/10.3390/fermentation6040097.

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Lactic acid is a building block for polylactic acid, which is one of the most promising polymers based on renewable resources and is used mainly in packaging industry. This bio-based polymer is biodegradable and provides an ecological and economical alternative to petrochemical plastics. The largest cost blocks of biotechnological lactic acid production, accounting for up to 38% of the total costs, are substrate and nutrient sources, such as peptone, meat, and yeast extract. Based on a systematic analysis of nutritional requirements, the substitution of yeast extract by low-cost protein-rich a
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Ahmad, Manzoor, Juan Ling, Jianping Yin, et al. "Evaluation of the Different Nutritional and Environmental Parameters on Microbial Pyrene Degradation by Mangrove Culturable Bacteria." International Journal of Molecular Sciences 24, no. 9 (2023): 8282. http://dx.doi.org/10.3390/ijms24098282.

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Mangrove ecosystems play curial roles in providing many ecological services and alleviating global climate change. However, they are in decline globally, mainly threatened by human activities and global warming, and organic pollutants, especially PAHs, are among the crucial reasons. Microbial remediation is a cost-effective and environmentally friendly way of alleviating PAH contamination. Therefore, understanding the effects of environmental and nutritional parameters on the biodegradation of polycyclic aromatic hydrocarbons (PAHs) is significant for the bioremediation of PAH contamination. I
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Zhong, Yuying, Wenhuang Zheng, Xinguo Shi, et al. "Pilot-Scale Fermentation of Pseudoalteromonas sp. Strain FDHY-MZ2: An Effective Strategy for Increasing Algicidal Activity." Biology 12, no. 11 (2023): 1447. http://dx.doi.org/10.3390/biology12111447.

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The role of microorganisms in effectively terminating harmful algal blooms (HABs) is crucial for maintaining environmental stability. Recent studies have placed increased emphasis on bio-agents capable of inhibiting HABs. The bacterium Pseudoalteromonas sp. strain FDHY-MZ2 has exhibited impressive algicidal abilities against Karenia mikimotoi, a notorious global HAB-forming species. To augment this capability, cultures were progressively scaled from shake flask conditions to small-scale (5 L) and pilot-scale (50 L) fermentation. By employing a specifically tailored culture medium (2216E basal
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Billah, MB, MM Sikder, MRI Mallik, MK Hossain, and N. Alam. "Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures." International Journal of Agricultural Research, Innovation and Technology 11, no. 2 (2022): 124–32. http://dx.doi.org/10.3329/ijarit.v11i2.57265.

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Present studies were conducted to isolate and identify the seed-borne pathogenic fungus from the selected tomato variety through morphological and molecular techniques based on the sequencing of internal transcribed spacer (ITS) region of 18S rDNA. According to the colony and conidial features, the fungus was identified as Curvularia sp. The obtained ITS sequencing showed above 99% similarity with Curvularia lunata in the NCBI database. The sequence of the fungus was deposited in NCBI GenBank under the accession number: ITS, MH382879.1. Besides, the phylogenetic tree further confirmed the taxo
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34

Vieira, J. N., A. M. Feijó, M. E. Bueno, et al. "Evaluation of the frequency of Candida spp. in hospitalized and non-hospitalized subjects." Brazilian Journal of Biology 78, no. 4 (2018): 644–52. http://dx.doi.org/10.1590/1519-6984.169623.

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Abstract The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours. The morphology identification was performed through m
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35

Rezgui, Raja, Abderrazak Maaroufi, Marie-Laure Fardeau, et al. "Anaerosalibacter bizertensis gen. nov., sp. nov., a halotolerant bacterium isolated from sludge." International Journal of Systematic and Evolutionary Microbiology 62, Pt_10 (2012): 2469–74. http://dx.doi.org/10.1099/ijs.0.036566-0.

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A strictly anaerobic, halotolerant and thermotolerant strain, designated C5BELT, was isolated in north Tunisia from storage tanks holding waste generated by the recycling of discarded motor oils. Cells of strain C5BELT were Gram-stain-positive, motile by laterally inserted flagella, straight, and spore-forming. Their two major fatty acids were iso-C15 : 0 and iso-C15 : 0 dimethyl acetal. Growth was observed at temperatures of 25–55 °C (optimum, 40 °C) and at pH 6–9 (optimum, pH 7.5). The salinity range for growth was 0–100 g l−1 NaCl (optimum, 5 g l−1). Yeast extract was required for growth. S
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Lauer, Wendy F., Sylvie Tymciu, Caroline D. Sidi, and Pierre Sonigo. "Validation of iQ-Check E. coli O157:H7 Real-Time PCR Test Kit for Detection of Escherichia coli O157:H7 in Selected Foods." Journal of AOAC INTERNATIONAL 92, no. 4 (2009): 1095–104. http://dx.doi.org/10.1093/jaoac/92.4.1095.

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Abstract iQ-Check E. coli O157:H7 (Bio-Rad Laboratories, Hercules, CA) is a real-time PCR kit for detection of E. coli O157:H7 from selected foods. Specific fluorescent oligonucleotide probes are used to detect target DNA during the amplification, by hybridizing to the amplicons. These fluorescent probes are linked to a fluorophore which fluoresces only when hybridized to the target sequence. Three foods (ground beef, apple cider, fresh spinach) were selected to compare the performance of iQ-Check E. coli O157:H7 to the U.S. Department of Agriculture-Food Safety and Inspection Service Microbio
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Sultana, Sanjida, Md Maniruzzaman Sikder, Farzana Ashrafi Neela, and Nuhu Alam. "First Report of Strawberry Leaf Blight Caused by Fusarium Fujikuroi Species Complex in Bangladesh." Journal of Bio-Science 30, no. 1 (2023): 11–20. http://dx.doi.org/10.3329/jbs.v30i1.63097.

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Strawberry leaf blight is one of the devastating fungal diseases, responsible for reducing the quality of strawberry production worldwide. The present experiment was conducted to determine the pathogenic fungal association with strawberry leaves obtained from the experimental site of Botanical Garden, Jahangirnagar University, Bangladesh. A fungal pathogen causing leaf blight disease of the strawberry plant was detected as Fusarium fujikuroi species complex through classical and molecular approaches. Molecular characterization showed that nucleotide sequences of the internal transcribed region
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Nagalla, A. D., D. V. Jayatilake, V. Herath, and L. D. B. Suriyagoda. "Evaluating the growth performance of Two Azotobactor spp. In liquid glucose broth and tryptic peptone broth as inoculum for the production of bio-fertilizers." Tropical Agricultural Research 28, no. 3 (2017): 312. http://dx.doi.org/10.4038/tar.v28i3.8234.

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Fu, Yu-Rong, Parushi Nargotra, Chia-Hung Kuo, and Yung-Chuan Liu. "Optimization of Biomass Cultivation from Tuber borchii and Effect of Additives on Triterpenoid Production." Fermentation 9, no. 8 (2023): 735. http://dx.doi.org/10.3390/fermentation9080735.

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Edible fungi are renowned for producing biologically active secondary metabolites that possess anti-tumor activity, protect the liver and have other benefits. The cultivation of truffle mycelia through submerged fermentation has gained interest in the production of metabolites for bio-medicinal purposes. In the present study, Tuber borchii was cultivated by submerged fermentation to produce both biomass and triterpenoids. Various additives, including palmitic acid, stearic acid, linoleic acid, chitosan, CaCl2 and limonene, were investigated to enhance triterpenoid production. It was observed t
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Sharma, SCD, MS Shovon, AKM Asaduzzaman, MG Sarowar Jahan, T. Yeasmin, and N. Roy. "Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp." Journal of Bio-Science 19 (December 19, 2012): 7–14. http://dx.doi.org/10.3329/jbs.v19i0.12994.

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Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out
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Tanvi, Amin, Joshi Disha, Patni Dhruvansh, and Mohil Meenakshi. "Harnessing the Proteolytic Power of Aspergillus Terreus Sgtm2." Indian Journal of Science and Technology 18, no. 12 (2025): 937–46. https://doi.org/10.17485/IJST/v18i12.3414.

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Abstract <strong>Objectives:</strong>&nbsp;The research is focused on isolating and characterizing proteolytic microorganisms from soil samples collected from a cowshed. Additionally, it aimed to optimize the conditions for protease production by utilizing a selected microbial strain.&nbsp;<strong>Methods:</strong>&nbsp;Cowshed soil were used to isolate protease-producing fungi; they were cultured on PDA (Potato Dextrose Agar) plates and tested for their ability to produce proteolytic enzymes using milk agar plates. Morphological analysis and 18S rRNA sequencing identified the strains. Submerg
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K, Kusumlata, Rajat Pratap Singh, and Ashish Kumar. "Biodecolorization of Azo Dye by Bacteria Alcaligenes faecalis Sub Sp. Phenolicus Isolated from a Bark-Beetle Tunnel Developed in Peltophorum Pterocarpum Plant." Current World Environment 19, no. 2 (2024): 824–40. http://dx.doi.org/10.12944/cwe.19.2.25.

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This study assessed the decolorization of reactive red 120 (RR120) by Alcaligenes faecalis subsp. phenolicus strain isolated from the bark borer insect (Indarbela tetraonis) tunnel developed in Peltophorum pterocarpum. The optimal parameters for the dye of decolorization 0.1 mg/L of dye were pH 7, temperature 35°C, fructose (0.4% w/v) as the carbon supply (0.4% w/v), peptone (0.2% w/v) as the nitrogen source (0.4% w/v), 12 hours of static conditions, and 0.3 ml of inoculums. Cell suspension, sodium alginate (3%, w/v), and PVA (5%, w/v) immobilized cell beads (10 beads 0.5 mm in size) were used
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Alozie, A., John, C. I., Hanis, B., Egbulefu, C. S., and Mbambam, F. "Investigation of the Potentials of Local Strains of Pseudomonas for Aquaculture Water Treatment." African Journal of Agricultural Science and Food Research 18, no. 1 (2025): 100–111. https://doi.org/10.62154/ajasfr.2025.018.010653.

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The need for human and animal food production cannot be over-emphasized. Aquaculture and related activities have become an important sustainable business in Nigeria. Water pollution rates have increased in modern times owing to the various chemical usage. The receiving water bodies therefore constitute a potential source of danger to both humans and animals. Several metabolites of the bacteria can cause detoxification of polluted water. There is a need to develop indigenous bacteria consortia for application in the treatment of wastewater bodies in order to reduce importation and earn local ea
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Ogunlaja, A., B. J. Ibidunni, K. Oyende, and O. O. Ogunlaja. "Optimization of bioflocculant production by bacteria isolated from oil-polluted soil and fermented maize effluent." Ife Journal of Science 22, no. 2 (2020): 201–10. http://dx.doi.org/10.4314/ijs.v22i2.18.

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This study involved isolation of bioflocculant producing bacteria from soil and waste water. The isolates were tested for flocculation activities and those deemed fit were identified and the optimal environmental conditions for bioflocculant production were also determined. Samples were collected from oil-contaminated soil in Redeemer's University and fermented maize waste water. Microbial isolation was done using standardmicrobiological methods and identification was done using morphology, biochemical and molecular method with universal primer for 16SrRNA gene. Environmental conditions (pH, T
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Adnan, Mohd, Arif Jamal Siddiqui, Syed Amir Ashraf, et al. "Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization." Polymers 14, no. 19 (2022): 3982. http://dx.doi.org/10.3390/polym14193982.

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Recently, there has been significant interest in bio-based degradable plastics owing to their potential as a green and sustainable alternative to synthetic plastics due to their biodegradable properties. Polyhydroxybutyrate (PHB) is a biodegradable polymer that is produced by bacteria and archaea as carbon and energy reserves. Due to its rapid degradation in natural environments, it can be considered a biodegradable plastic alternative. In the present study, a dye-based procedure was used to screen PHB-producing bacteria isolated from mangrove soil samples. Among the seven isolates, Agromyces
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46

Rezgui, Raja, Zouhaier Ben Ali Gam, Said Ben Hamed, et al. "Sporosalibacterium faouarense gen. nov., sp. nov., a moderately halophilic bacterium isolated from oil-contaminated soil." International Journal of Systematic and Evolutionary Microbiology 61, no. 1 (2011): 99–104. http://dx.doi.org/10.1099/ijs.0.017715-0.

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A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37T, was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37T stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37T had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C15 : 0 (41 %), iso-C14 : 0 3-OH and/or iso-C15 : 0 dimethyl acetal (21.6 %), iso-C13 : 0 (
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Karaca, Banu Taktak, James Meyer, Sarah VanOosten, Mark Richter, and Candan Tamerler. "Modular Peptide-Based Hybrid Nanoprobes for Bio-Imaging and Bio-Sensing." MRS Proceedings 1621 (2014): 155–61. http://dx.doi.org/10.1557/opl.2014.368.

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ABSTRACTThe self-organization of functional proteins directly onto solid materials is attractive to a wide range of biomaterials and systems that need to accommodate a biological recognition element. In such systems, inorganic binding peptides may be an essential component due to their high affinity and selective binding features onto different types of solid surfaces. This study demonstrates a peptide-enabled self-assembly technique for designing well-defined protein arrays over a metal surface. To illustrate this concept, we designed a fusion protein that simultaneously displays a red fluore
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Zhang, Weihong, and Peng Yang. "2D bio-nanostructures fabricated by supramolecular self-assembly of protein, peptide, or peptoid." Advanced Composites and Hybrid Materials 2, no. 2 (2018): 201–13. http://dx.doi.org/10.1007/s42114-018-0066-x.

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Apostolopoulos, Vasso, Joanna Bojarska, Tsun-Thai Chai, et al. "A Global Review on Short Peptides: Frontiers and Perspectives." Molecules 26, no. 2 (2021): 430. http://dx.doi.org/10.3390/molecules26020430.

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Peptides are fragments of proteins that carry out biological functions. They act as signaling entities via all domains of life and interfere with protein-protein interactions, which are indispensable in bio-processes. Short peptides include fundamental molecular information for a prelude to the symphony of life. They have aroused considerable interest due to their unique features and great promise in innovative bio-therapies. This work focusing on the current state-of-the-art short peptide-based therapeutical developments is the first global review written by researchers from all continents, a
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Tognarelli, David, Atsushi Tsukamoto, Jeff Caldwell, and Walt Caldwell. "Rapid peptide separation by supercritical fluid chromatography." Bioanalysis 2, no. 1 (2010): 5–7. http://dx.doi.org/10.4155/bio.09.165.

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