Academic literature on the topic 'BlaNDM-1 gene'

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Journal articles on the topic "BlaNDM-1 gene"

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Xin, Xueqiang, Yi Yin, Jiayong Kong, Mianzhi Wang, Zhiqiang Wang та Ruichao Li. "Genomic Insights into Carbapenem-Resistant Organisms Producing New Delhi Metallo-β-Lactamase in Live Poultry Markets". Microorganisms 13, № 6 (2025): 1195. https://doi.org/10.3390/microorganisms13061195.

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New Delhi metallo-β-lactamase (NDM) is an enzyme that can degrade a wide range of β-lactam antibiotics. The widespread dissemination of the blaNDM gene, which encodes NDM, in animal-derived settings poses a threat to public health security. Live poultry markets represent critical nodes in public health surveillance. However, there is currently limited reporting on the spread of the blaNDM gene within these markets under the One Health approach. This study investigated the prevalence of the blaNDM gene in live poultry markets and, by integrating newly sequenced genomes with publicly available d
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Kong, Ling-Han, Rong Xiang, Yu-Long Wang, et al. "Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris." Journal of Antimicrobial Chemotherapy 75, no. 6 (2020): 1439–42. http://dx.doi.org/10.1093/jac/dkaa068.

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Abstract Objectives To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin. Methods The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018. The presence of carbapenemase genes was screened by PCR. WGS and bioinformatics analysis were performed to analyse the genetic environment of the carbapenem resistance determinant. Results P. vulgaris strain BC22 was found to harbour the carbapenemase gene blaNDM-1. WGS data revealed that blaNDM-1 w
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Bello-López, Elena, Ángeles Pérez-Oseguera, Walter Santos, and Miguel Ángel Cevallos. "A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene." PLOS ONE 19, no. 5 (2024): e0303976. http://dx.doi.org/10.1371/journal.pone.0303976.

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The blaNDM-1 gene and its variants encode metallo-beta-lactamases that confer resistance to almost all beta-lactam antibiotics. Genes encoding blaNDM-1 and its variants can be found in several Acinetobacter species, and they are usually linked to two different plasmid clades. The plasmids in one of these clades contain a gene encoding a Rep protein of the Rep_3 superfamily. The other clade consists of medium-sized plasmids in which the gene (s) involved in plasmid replication initiation (rep)have not yet been identified. In the present study, we identified the minimal replication region of a b
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Naeem, Sohni, Hazrat Bilal, Hafsah Muhammad, et al. "Detection of blaNDM-1 gene in ESBL producing Escherichia coli and Klebsiella pneumoniae isolated from urine samples." Journal of Infection in Developing Countries 15, no. 04 (2021): 516–22. http://dx.doi.org/10.3855/jidc.12850.

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Introduction: Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae are the most prominent bacterial species resistant to almost all commonly used antibiotics. Carbapenem is one of the last resort drugs for treating such emerging multidrug-resistant bacteria. This study aimed to detect carbapenem-resistant blaNDM-1 gene in ESBL producing E. coli and K. pneumoniae isolates.
 Methodology: A total of 190 E. coli and 350 K. pneumoniae isolates were screened for extended spectrumβ-lactamase (ESBL), carbapenemase and metallo β-lactamase (MBL) production via double-disk synergy t
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Hussein, Zeyad, Haider Kadhim, and Jabbar Hassan. "DETECTION OF NEW DELHI METALLO-BETA-LACTAMASE-1 (BLANDM-1) IN CARBAPENEM-RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SAMPLES IN WASIT HOSPITALS." Iraqi Journal of Medical Sciences 16, no. 3 (2018): 239–46. http://dx.doi.org/10.22578/ijms.16.3.3.

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Background:Pseudomonas aeruginosa (P. aeruginosa) infections are clinical problem, it is a difficult to treat because of high resistant to many antibiotics (Multi-drug resistant) and a high risk of emergence of resistance during therapy. Carbapenems are therapeutic choice against infections caused by Gram-negative bacilli including strains of P. aeruginosa. New Delhi metallo-β-lactamase-1 (blaNDM-1) gene, an acquired class B carbapenemase. Dissemination predominantly involves transfer of the blaNDM-1 gene among promiscuous plasmids and clonal outbreaks. Bacteria with NDM-1 are typically resist
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Bilal, Hazrat, Gaojian Zhang, Tayyab Rehman, et al. "First Report of blaNDM-1 Bearing IncX3 Plasmid in Clinically Isolated ST11 Klebsiella pneumoniae from Pakistan." Microorganisms 9, no. 5 (2021): 951. http://dx.doi.org/10.3390/microorganisms9050951.

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The New Delhi Metallo-β-lactamase (NDM) is among the most threatening forms of carbapenemases produced by K. pneumoniae, well-known to cause severe worldwide infections. The molecular epidemiology of blaNDM-1-harboring K. pneumoniae is not well elucidated in Pakistan. Herein, we aim to determine the antibiotics-resistance profile, genes type, molecular type, and plasmid analysis of 125 clinically isolated K. pneumoniae strains from urine samples during July 2018 to January 2019 in Pakistan. A total of 34 (27.2%) K. pneumoniae isolates were carbapenemases producers, and 23 (18.4%) harbored the
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Tsilipounidaki, Katerina, Christos-George Gkountinoudis, Zoi Florou, George C. Fthenakis, Vivi Miriagou, and Efthymia Petinaki. "The Molecular Characterization of blaNDM-1-Positive Acinetobacter baumannii Isolated in Central Greece." Microorganisms 11, no. 10 (2023): 2588. http://dx.doi.org/10.3390/microorganisms11102588.

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The objective of the present study is to report the detection and the molecular characterization of nine blaNDM-1-positive Acinetobacter baumannii isolates, which were isolated from patients in a tertiary care hospital in Central Greece from December 2022 to August 2023. The isolates were characterized by whole genome sequencing to obtain Pasteur multilocus sequencing typing (MLST) and to identify the blaNDM-1-environment, resistome, and virulence genes content. In silico MLST analysis showed that the isolates belonged to four different clones (STs 160, 2, 85, and 2493). All strains, apart fro
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Chen, Wenxin, Zhihong Liu, Hongguang Lin, et al. "Occurrence of blaNDM-1-Positive Providencia spp. in a Pig Farm of China." Antibiotics 11, no. 6 (2022): 713. http://dx.doi.org/10.3390/antibiotics11060713.

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Antibiotics have been extensively used to ensure the productivity of animals on intensive livestock farms. Accordingly, antimicrobial-resistant organisms, which can be transmitted to humans via the food chain, pose a threat to public health. The Enterobacterium antimicrobial resistance gene, blaNDM-1, is a transmissible gene that has attracted widespread attention. Here, we aimed to investigate the prevalence of Enterobacteriaceae carrying blaNDM-1 on an intensive pig farm. A total of 190 samples were collected from a pig farm in Hunan Province, China. Resistant isolates were selected using Ma
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Tanni, Afroza Akter, Md Mahbub Hasan, Nahid Sultana, Wazir Ahmed, and Adnan Mannan. "Prevalence and molecular characterization of antibiotic resistance and associated genes in Klebsiella pneumoniae isolates: A clinical observational study in different hospitals in Chattogram, Bangladesh." PLOS ONE 16, no. 9 (2021): e0257419. http://dx.doi.org/10.1371/journal.pone.0257419.

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Objective This study was performed to investigate the prevalence of multidrug resistance and molecular characterization of Klebsiella pneumoniae (KPN) from clinical isolates in the southern region of Bangladesh. Additional analysis of the prevalence of blaNDM-1, blaSHV-11, uge genes of KPN was also carried out among these clinical isolates. Method The study was carried out using 1000 clinical isolates collected from two different hospitals of Chattogram. A drug susceptibility test was performed by the disk diffusion method to detect KPN’s response to 16 antibiotics. The presence of antibiotic-
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Tula, M. Y., O. I. Enabulele, E. A. Ophori, R. O. Okojie та F. Joel. "Emergence of New Delhi metallo-β-lactamase-1 (NDM-1) producing Enterobacterales from water sources: An impending public health challenge in Adamawa-north senatorial zone, Nigeria". African Journal of Clinical and Experimental Microbiology 24, № 3 (2023): 258–65. http://dx.doi.org/10.4314/ajcem.v24i3.5.

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Background: The emergence of New Delhi metallo-beta-lactamase-1 (NDM-1) among Enterobacterales in water sources has raised a major public health concern and constitute critical threat to human health as these organisms exhibits high level of resistance to available potent antibiotics. The aim of this study is to detect the presence of NDM-1 gene among carbapenem resistant Enterobacterales (CRE) isolates from water sources.
 Methodology: A total of 256 water samples were collected from randomly selected hand-dug wells (128 samples) and river/stream (128 samples) for each of dry and rainy s
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Book chapters on the topic "BlaNDM-1 gene"

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Padhan, Monalisa, and Smaranika Pattnaik. "Prevalence of PDR bacterial strains harbored with blaNDM-1 gene isolated from UTI patients." In Contemporary Medical Biotechnology Research for Human Health. Elsevier, 2022. http://dx.doi.org/10.1016/b978-0-323-91251-8.00019-2.

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Conference papers on the topic "BlaNDM-1 gene"

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Lima, Keila Oliveira, Aline Valério de Lima, Fabiana Teixeira, Paola Cappellano, Suely Carlos Ferreira Sampaio, and Jorge Luiz Mello Sampaio. "CIM-CD – an efficient and low-cost test for detection and differentiation of Ambler classes A, B, and D carbapenemases in Klebsiella pneumoniae complex." In Resumos do 56º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2024. https://doi.org/10.5327/1516-3180.142s1.11182.

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Objective: Current phenotypic tests have limitations in detecting coproduction of carbapenemases from different Ambler classes. This study aimed to develop and validate a modified carbapenem inactivation method to address these challenges. Method: The following modifications were made: 1– use of an imipenem disk instead of meropenem; 2 – inclusion of an imipenem-relebactam disk; 3 – addition of dipicolinic acid (DPA) 4 – inclusion of Evans blue dye; 5 – use of an MF 3.0 suspension of E. coli ATCC 25922 for reading within 6 hours; 6 – adjustment of zinc sulfate (ZnSO4) concentration; 7 – inclus
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Lima, KO, AV Lima, and JLM Sampaio. "DETECÇÃO E CARACTERIZAÇÃO RÁPIDA DE COPRODUÇÃO DE CARBAPENEMASES E VARIANTES DE KPC EM KLEBSIELLA SPP." In Resumos do 55º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s2.8386.

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Objetivo: Desenvolver e validar um novo teste para detecção e diferenciação rápida de coprodução de carbapenemases. Método: Um total de 275 Klebsiella spp., produtoras de NDM, KPC, OXA-48-like ou coprodutoras de KPC/NDM ou NDM/OXA, teve suas espécies identificadas e a presença de genes de carbapenemases confirmada por PCR. Foram realizadas as seguintes modificações em relação ao método original CIM: 1. inclusão do imipenem-relebactam; 2. utilização de corante; 3. utilização de suspensão de E. coli ATCC 25922 com turbidez MF 3,0 para leitura em 6 horas; 4. inclusão da ceftazidima-avibactam CAZ-
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Oliveira, Érica Maria de, Nathaly Bruna de Oliveira Silva, and Ana Catarina de Souza Lopes. "OCORRÊNCIA DE blaKPC E blaVIM EM ISOLADOS DE Pseudomonas aeruginosa MULTIDROGA RESISTENTES PROVENIENTES DE INFECÇÃO EM PACIENTES DE UM HOSPITAL PÚBLICO EM RECIFEPE." In XXVII Semana de Biomedicina Inovação e Ciência. Editora IME, 2021. http://dx.doi.org/10.51161/9786588884119/45.

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Introdução: Pseudomona aeruginosa é uma bactéria gram negativa oportunista considerada uma séria ameaça à saúde pública e são responsáveis por uma variedade de Infecções Relacionadas à Assistência à Saúde (IRAS), como pneumonias, infecções em tecidos moles, no trato urinário, e bacteremias(1).A emergência de P. aeruginosa resistentes aos carbapenêmicos, produtora de carbapenemases, dificulta ainda mais o tratamento e são associadas a altas taxas de mortalidade(2). Objetivos: O objetivo desse estudo foi analisar o perfil de susceptibilidade bem como detectar a presença dos genes de resistência
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Bomfim, Isabela Maria Fortaleza Neves, Yanne Naara Teixeira De Carvalho, Joyce Hercília Jerônimo Lins, Amanda Viera Barros, and Renato Motta Neto. "CULTURAS DE VIGILÂNCIA DE RESISTÊNCIA DE PACIENTES INTERNOS EM UNIDADE DE TERAPIA INTENSIVA DO HOSPITAL REFERÊNCIA EM DOENÇAS INFECCIOSAS DO RIO GRANDE DO NORTE." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1202.

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Introdução: As Infecções Relacionadas à Assistência à Saúde (IRAS) são infeções adquiridas no curso de uma internação em uma unidade hospitalar. As Unidades de Terapia Intensiva (UTI) são os locais onde frequentemente ocorrem surtos por bactérias multirresistentes, visto a pressão seletiva e a disseminação de genes de resistência nestes ambientes. As culturas de vigilância de resistência são um conjunto de técnicas de isolamento e identificação de microrganismos resistentes aos antimicrobianos em indivíduos colonizados com a finalidade de prevenir as IRAS. Objetivos: Este trabalho teve como me
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Nurmberger, Jussimara Monteiro, Fernanda Matsiko Inoue, Samuel Gerson Macedo Vieira, Thais Turati, Ana Paula Timm Lobo, and Sergio Tufik. "Epidemiologia molecular de Enterobacterales carreadora de dupla carbapenemase (KPC e NDM): uma nova realidade brasileira." In Resumos do 56º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2024. https://doi.org/10.5327/1516-3180.142s1.10963.

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Objetivo: O objetivo deste estudo foi descrever o aumento de isolados clínicos de Enterobacterales produtoras de dupla carbapenemases recuperados de pacientes atendidos em 35 centros de saúde, distribuídos em sete estados brasileiros. Método: Desde 2012, o setor de microbiologia clínica tem realizado o teste de triagem para pesquisa de carbapenemases. A identificação bacteriana foi realizada por espectrometria de massa, e a concentração inibitória mínima de antibióticos, por sistema automatizado, exceto para polimixina B, o qual foi utilizado microdiluição em caldo. A detecção dos genes de car
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