Relevant bibliographies by topics / Blastocyst hatching process

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Academic literature on the topic 'Blastocyst hatching process'

Author: Grafiati
Published: 6 September 2023
Last updated: 31 July 2025

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Journal articles on the topic "Blastocyst hatching process"

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Cheon, Yong Pil, Myung Chan Gye, Chung-hoon Kim, et al. "Role of actin filaments in the hatching process of mouse blastocyst." Zygote 7, no. 2 (1999): 123–29. http://dx.doi.org/10.1017/s0967199499000477.

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Hatching has been suggested to occur as a result of protease-mediated lysis and the blastocoele tension. However, even if rupturing is initiated at multiple sites, interestingly only a single site is used for escape. This implies that there are several mechanisms involved in hatching. In this study, the involvement of actin filaments in mouse embryo hatching was examined. We treated mouse embryos with cytochalasin B for 12 h or 24 h at the morula, middle blastocyst, expanded blastocyst, lobe-formed blastocyst and hatching blastocyst stages, and measured the amount and distribution of actin fil
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Kim, Y. J., K. S. Ahn, S. M. Park, B. C. Lee, H. Shim, and C. Ahn. "28 IMPROVED HATCH RATE AFTER PARTIAL DISSECTION OF ZONA PELLUCIDA IN CLONED PIG EMBRYO." Reproduction, Fertility and Development 29, no. 1 (2017): 121. http://dx.doi.org/10.1071/rdv29n1ab28.

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For last 20 years, the efficiency of animal cloning has remained extremely low, despite many attempts to improve it. Although nuclear transfer experiments have been almost optimized, artificial holes are inevitably made in the zona pellucida (ZP) during nuclear transfer experiments, such as enucleation of maternal genome or injection of nuclear donor. Hatching from the ZP is a prerequisite for mammalian embryo implantation, and the condition of the ZP has a lot of influence on hatching. The present studies were performed to investigate the effects of artificial holes in the ZP, because of the
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Eswari, S., G. Sai Kumar та G. Taru Sharma. "Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation". Zygote 21, № 2 (2012): 203–13. http://dx.doi.org/10.1017/s0967199412000172.

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SummaryThe objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus–oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo s
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Leonavicius, Karolis, Christophe Royer, Chris Preece, Benjamin Davies, John S. Biggins, and Shankar Srinivas. "Mechanics of mouse blastocyst hatching revealed by a hydrogel-based microdeformation assay." Proceedings of the National Academy of Sciences 115, no. 41 (2018): 10375–80. http://dx.doi.org/10.1073/pnas.1719930115.

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Mammalian embryos are surrounded by an acellular shell, the zona pellucida. Hatching out of the zona is crucial for implantation and continued development of the embryo. Clinically, problems in hatching can contribute to failure in assisted reproductive intervention. Although hatching is fundamentally a mechanical process, due to limitations in methodology most studies focus on its biochemical properties. To understand the role of mechanical forces in hatching, we developed a hydrogel deformation-based method and analytical approach for measuring pressure in cyst-like tissues. Using this appro
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Morató, Roser, Míriam Castillo-Martín, Marc Yeste, and Sergi Bonet. "Cryotolerance of porcine in vitro-produced blastocysts relies on blastocyst stage and length of in vitro culture prior to vitrification." Reproduction, Fertility and Development 28, no. 7 (2016): 886. http://dx.doi.org/10.1071/rd14203.

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The aim of our study was to assess whether the cryotolerance of in vitro-produced embryos could be influenced by the length of in vitro culture and size of blastocoel cavity before vitrification, using the pig as a model. For this purpose we analysed the cryoresistance and apoptosis rate of blastocysts at different stages of development as derived on Day 5 and 6 of in vitro culture. Blastocysts were subsequently vitrified, warmed and cultured for 24 h. Re-expansion rates were recorded at 3 and 24 h and total cell number and apoptotic cells were determined at 24 h. Day-6 blastocysts showed the
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6

Pasquariello, R., J. R. Herrick, Y. Yuan, et al. "73 Fatty Acid Supplementation in Culture Medium with Reduced Nutrient Concentrations Improves Bovine Blastocyst Development Compared with Standard Culture Medium." Reproduction, Fertility and Development 30, no. 1 (2018): 175. http://dx.doi.org/10.1071/rdv30n1ab73.

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Lipids are a potent source of cellular energy and are metabolized within mitochondria via fatty acid β-oxidation, a process that also requires carnitine. Embryos metabolize lipids during pre-implantation development, but relatively little is known about the effect of fatty acid supplementation for early bovine embryogenesis in culture. The objective of this study was to evaluate the effect of lipid supplementation (via albumin) and l-carnitine (C; 5 mM) during embryo culture in a novel medium with reduced concentrations of nutrients, compared with our standard culture medium (control). Followi
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7

Fujishima, Ayaka, Kazumasa Takahashi, Mayumi Goto, et al. "Analysis of Na+ concentration patterns in trophectoderm cells of mouse blastocysts using a dual-wavelength electrolyte indicator." PLOS One 20, no. 4 (2025): e0322286. https://doi.org/10.1371/journal.pone.0322286.

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The developmental process of the mammalian blastocyst involves the intricate interplay of cellular and molecular mechanisms, including electrolyte dynamics within the trophectoderm (TE). We hypothesized that sodium (Na+) is actively transported from the TE into the blastocyst cavity, driving water influx and promoting blastocyst expansion. In this study, we investigated the dynamics of Na+ concentration in the TE of mouse embryos using sodium-binding benzofuran isophthalate (SBFI), a dual-wavelength Na+-sensitive fluorescent indicator. Observations revealed three distinct patterns of Na+ dynam
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8

Ribeiro, E. S., M. C. Gonçalves, M. C. Pedrotti, et al. "74 EFFECT OF BETA-MERCAPTOETHANOL ON THE VITRIFICATION CRYOTOLERANCE OF BOVINE IN VITRO-PRODUCED EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 137. http://dx.doi.org/10.1071/rdv21n1ab74.

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The control of oxidative processes in in vitro production (IVP) systems by the use of additives may be an alternative approach to improve embryo cryotolerance. The aim of this study was to verify the effect of β-mercaptoethanol (βME) on the cryotolerance of bovine IVP embryos. In 7 replications, and following IVM-IVF, presumptive zygotes (n = 3735) were in vitro-cultured in SOF medium supplemented or not with 100 μm βME (IVC treatment), at 38.5°C and high humidity. The initial 24 h of IVC was performed in 5% CO2 in air, with the remaining 6 days of IVC carried out in 5% CO2, 5% O2, and 90% N2.
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Silva, Thiago Velasco Guimarães, Priscila Di Paula Bessa Santana, Eduardo Baia de Souza, et al. "Sperm chromatin protamination influences embryo development in unsexed and sexed bull semen." Zygote 29, no. 4 (2021): 264–69. http://dx.doi.org/10.1017/s0967199420000775.

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SummarySex selection through sperm sorting offers advantages in regards selection pressure in high-producing livestock. However, the sex-sorting process results in sperm membrane and DNA damage that ultimately decrease fertility. We hypothesized that given the role of protamines in DNA packaging, protamine deficiency could account, at least partially, for the DNA damage observed following sperm sex sorting. To test this, we compared protamine status between unsexed and sexed spermatozoa from two bulls using the fluorochrome chromomycin A3 (CMA3) and flow cytometry. Then, we assessed embryo dev
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10

Rascado, T. S., M. D. Guastali, D. M. Paschoal, et al. "208 COMPARISON OF THE EXPRESSION OF SOX2 AND Stat3 IN BOVINE BLASTOCYST AND HATCHED BLASTOCYST." Reproduction, Fertility and Development 25, no. 1 (2013): 252. http://dx.doi.org/10.1071/rdv25n1ab208.

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The investigation of the transcription factors involved in the regulation of pluripotency in pre-implantation embryos of cattle provides important information regarding the early embryonic development and derivation of embryonic stem cells. The present experiment aimed to compare the level of expression of SOX 2 and Stat3, pluripotency markers, in bovine blastocysts (D7) and hatched blastocysts (D10), because it is known that around hatching, the inner cell mass (ICM) differentiates into hypoblast and epiblast. Cumulus–oocyte complexes were matured in TCM 199 for 24 h and fertilized with froze
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Dissertations / Theses on the topic "Blastocyst hatching process"

1

Pathak, Madhulika. "Embryonic Expression of Cytokines: Functional Role of Il-1β During Blastocyst Hatching". Thesis, 2020. https://etd.iisc.ac.in/handle/2005/4530.

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The detailed investigation on the embryo-endometrial cytokine expression, in both mouse and hamster, was carried out during the peri-hatching development and it is described in chapter 2. We hypothesize that, for a successful implantation, a balance of embryonic expression and function of pro-inflammatory and anti-inflammatory cytokines are necessary to maintain proper homeostasis of cytokine expression, compatible with peri- hatching embryonic development and hatching, eventually enabling the establishment of early pregnancy. Our cytokine expression studies along with various other reports
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