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Dissertations / Theses on the topic 'Cell culture'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Liu, Mengfei, and 刘梦菲. "Epithelial morphogenesis in three-dimensional cell culture system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208611.

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In human body, the most common structures formed by epithelial cells are hollow cysts or tubules. The key feature of the cysts and tubules is the central lumen, which is lined by epithelial cell sheets. The central lumen allows material exchange, thus it is indispensable for the proper function of the epithelial tissue. In order to understand the way that the epithelial cells form highly specialized structure, an in vitro three-dimensional (3D) culture system was established. The Caco-2 cells were embedded in reconstituted basement membrane termed matrigel, whose biochemical constitution and

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2

Theil, Ian. "Anchorage-dependent mammalian cell culture." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56768.

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Genetically engineered anchorage-dependent human embryonic kidney (293) cells were cultured at 37$ sp circ$C on 1 mm thick sheets of a fibrous polymeric matrix having an average fibre diameter of 10.2 $ mu$m and a void fraction of 0.81 using Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2.5 mM glutamine. Immobilization efficiencies above 70% were observed when cells were added to 100 mL spinner flasks (operating at 60 rpm) containing 70 mL of medium and two 1 x 1 cm squares of matrix (total gross area of 2 cm$ sp2$) fastened to the base of the sti

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3

Machado, Roque Ana Isabel. "Protein scaffolds for cell culture." Thesis, University of Newcastle Upon Tyne, 2013. http://hdl.handle.net/10443/1843.

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We report here the design, purification and structural characterization of a new protein scaffold for cell culture. Prior studies in our group revealed the structure of the bacterial protein Caf1 to be flexible protein nanofibres, up to 1.5 μm. The existing Cafl expression system was cumbersome and difficult to mutate, we have now produced a system containing the caf operon which allows for the incorporation of specific peptide motifs. The small peptide, RGDS from fibronectin was inserted into 5 different surface loops of Caf1. The Caf1 mutants were expressed and purified and a structural char

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4

Lee, Kevin Shao-Kwan. "Microscale controlled continuous cell culture." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/64579.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 489-500).<br>Measurements of metabolic and cellular activity through substrate and product interactions are highly dependent on environmental conditions and cellular metabolic state. For such experiments to be feasible, continuous cultures are utilized to ensure consistent conditions. However, since medium must be replenished every cell doubling time, costs can be prohibitive in large reactors. An

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5

Schley, Jeremiah P. "Single Cell Culture Wells (SiCCWells)." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406292709.

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6

Balagopal, Tulika C., Xiao Lucy Cheng, Jessica Gamboa, John Harper, Sheridan McPheeters, and Bryce Notheis. "A Dynamic Cell Culture System." Thesis, The University of Arizona, 2010. http://hdl.handle.net/10150/146852.

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The following includes information regarding the design, construction, and initial testing of A Dynamic Cell Culture System. This project has been undertaken in order to provide the Wu Lab with a tool that will allow a method to more accurately simulate human vasculature for research purposes by applying pulsatile shear, tensile, and normal pressures. This is accomplished by utilizing upper and lower Chambers between which reside tissue scaffolds (composed of silk and elastin) which are seeded with cells. A high flow-rate pump is used to pass cell culture media over the scaffolds in order to c

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7

Reiland, Joanne Elizabeth Donovan Maureen D. "Analysis of cell culture models of mammary drug transport." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/316.

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8

Ekström, Jens-Ola. "Ljungan Virus Replication in Cell Culture." Doctoral thesis, Högskolan i Kalmar, Naturvetenskapliga institutionen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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Ljungan virus (LV) is a recently identified picornavirus of the genus Parechovirus. LV has been isolated from voles trapped in Sweden and also in the United States. LV infected small rodents may suffer from diabetes type 1 and type 2 like symptoms, myocarditis and encephalitis. LV has been proposed as a human pathogen, with indications of causing diabetes type 1, myocarditis and intrauterine fetal deaths. In this thesis, cell culture adapted LV strains were utilised for development and adaptation of several basic methodological protocols to study the LV biology, e.g. real time PCR, highly spec

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9

Ekström, Jens-Ola. "Ljungan virus replication in cell culture /." Kalmar : University of Kalmar, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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10

Jayawarna, Vineetha. "Fmoc-peptide gels for cell culture." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489519.

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Spontaneous formation of macroscopic hydrogels from small molecule building blocks via self-assembly is a powerful tool for the preparation of novel materials with well defined properties. Peptides are particularly interesting as building blocks for these materials. Self-assembled nanowires, fibres, sheets and tubes from peptide systems have all been described.

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11

Johnstone, Alex. "Microfluidic systems for neuronal cell culture." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/37822/.

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At a high level of abstraction, the brain is a system for analysing sensory information, and responding appropriately. That information is encoded and stored in the millions of neural circuits that comprise the brain. Deciphering this code is essential to understanding how memories are implemented in physiologically normal brain tissue, and to inferring the nature of some neurological disorders affecting memory such as Alzheimer’s disease, in which the neural encoding is aberrant or unsuccessful. One approach to this problem is to reduce the complexity of the brain functionality to three eleme

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12

Murphy, Eric James. "Cell culture models for neural trauma /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487672245901403.

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13

Reichen, M. "Multiplexed microfabricated cell culture device for stem cell process development." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1349012/.

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In this thesis, a multiplexed micro-fabricated cell culture device is presented to probe the soluble micro-environment of stem cells. A large number of biological, physical and chemical variables determine the micro-environment of stem cells and affect therefore their fate. The soluble micro-environment is believed to play a pivotal role in controlling stem cell fate and its optimisation may therefore allow exploitation of applications such as regenerative medicine or drug discovery. However, novel tools are required to probe and optimise the soluble micro-environment. The ability to move smal

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14

Berdugo, Claudia. "Cell Damage Mechanisms and Stress Response in Animal Cell Culture." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1269467441.

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15

Kim, Narae. "External pH in culture on somatic cell reprogramming and cell differentiation in mouse and chicken cells." Kyoto University, 2017. http://hdl.handle.net/2433/218018.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第20092号<br>農博第2199号<br>新制||農||1046(附属図書館)<br>学位論文||H29||N5026(農学部図書室)<br>33208<br>京都大学大学院農学研究科応用生物科学専攻<br>(主査)教授今井裕, 教授松井徹, 教授久米新一<br>学位規則第4条第1項該当

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16

Capra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.

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Abstract The epithelial cells form barriers that compartmentalize the organs. Carcinomas are cancers stemming from epithelial cells and are the most common cancer type. The aim of this study was to understand the differentiation and malignant transformation of epithelial Madin-Darby canine kidney (MDCK) cells and to analyse the electrophysiological parameters which regulate their transport capacity. Emphasis was placed on comparing different culture environments, both in 2D and 3D. First, the effects of drugs or basal extracellular fluid composition on MDCK cell, cyst and lumen volumes were an

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17

Huynh, Minh Diem. "Reduction in apparent stromal cell culture density through transient fusions with osteosarcoma cells." Thesis, The University of Sydney, 2007. http://hdl.handle.net/2123/4465.

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Benign tumours grow by expanding and displacing the surrounding tissues, while malignant tumours replace and destroy the surrounding tissues by invasion. Although there is extensive literature on mechanisms of tumour invasion and metastasis, with an emphasis on angiogenesis, adhesion, degradation of the extracellular matrix and migration, an important question not clearly addressed by the literature, but nonetheless approached in this thesis, is that of the fate of normal cells during tissue replacement by migrating invasive malignant cells. Earlier work in the laboratory where this PhD candid

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18

Huynh, Minh Diem. "Reduction in apparent stromal cell culture density through transient fusions with osteosarcoma cells." University of Sydney, 2007. http://hdl.handle.net/2123/4465.

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Doctor of Philosophy<br>Benign tumours grow by expanding and displacing the surrounding tissues, while malignant tumours replace and destroy the surrounding tissues by invasion. Although there is extensive literature on mechanisms of tumour invasion and metastasis, with an emphasis on angiogenesis, adhesion, degradation of the extracellular matrix and migration, an important question not clearly addressed by the literature, but nonetheless approached in this thesis, is that of the fate of normal cells during tissue replacement by migrating invasive malignant cells. Earlier work in the laborato

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19

Oliver, Nicole Ann. "Developing a robust harvest for high cell density CHO cell culture." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/130614.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Biological Engineering, May, 2020<br>Thesis: M.B.A., Massachusetts Institute of Technology, Sloan School of Management, in conjunction with the Leaders for Manufacturing Program at MIT, May, 2020<br>Cataloged from the official PDF version of thesis.<br>Includes bibliographical references (pages 96-98).<br>by Nicole Ann Oliver.<br>S.M.<br>M.B.A.<br>S.M. Massachusetts Institute of Technology, Department of Biological Engineering<br>M.B.A. Massachusetts Institute of Technology, Sloan School of Management

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20

Sutton, Amy. "A New Active Cell Culture Material for Controlled Cell Micro-Manipulation." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493600.

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Mechanical forces in the cell’s natural environment have a crucial impact on growth and behavior, from single-cell gene expression and cell division to the spatial patterning of complex organ architectures. Few areas of biology can be understood without taking into account how both individual cells and networks of cells sense and transduce physical stresses as part of a tightly-controlled and highly integrated, hierarchical collective. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on cells, particularly w

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21

Vajrala, Sucheta Gowthami. "Mechanism of CO2 inhibition in insect cell culture." Thesis, University of Iowa, 2010. https://ir.uiowa.edu/etd/611.

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The prominence of insect cell culture has grown rapidly due to its ability to produce baculovirus biopesticides and recombinant proteins using the Baculovirus Expression Vector System. A critical problem in the mass production of these products is CO2 accumulation to inhibitory levels within the bioreactor. The current research investigated the effect of elevated CO2 concentrations on insect cell growth and metabolism and the roles of oxidative stress and intracellular pH (pHi) in CO2 inhibition. Spodoptera frugiperda Sf-9 insect cells were cultured in a 3 L bioreactor (1.2 L working volume) c

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22

Sjögren, Frida. "Microstructuring of Hyaluronic acid cell culture scaffolds." Licentiate thesis, Uppsala universitet, Mikrosystemteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-334653.

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23

Chen, Michael C. W. "Hydrogel-based microfluidic system for cell culture." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/7209.

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Traditionally, cell culture has been done in culture flasks or well plates where the volumes and length scales involved in the culture environment are many orders of magnitude larger than the size scale of individual cells. To better tailor medical care to an individual patient, it may be necessary to carry out genetic, physiological, and biochemical analyses on very small cell samples and to have an in vitro cell culture environment that more closely approximates the in vivo conditions. A microfluidic device that integrates both cell handling and long-term 3-D cell culture techniques is p

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24

Lamb, Keith A. "Cell culture models for transdermal drug delivery." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334854.

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25

Zupke, Craig Allen. "Metabolic flux analysis in mammalian cell culture." Thesis, Massachusetts Institute of Technology, 1993. http://hdl.handle.net/1721.1/12661.

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26

Macown, R. J. "Microfabricated devices for adherent stem cell culture." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1465828/.

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This thesis details the development of a system of microfabricated devices for the adherent culture of stem cells. The multipotency and self renewal of stem cells make them a potentially abundant source of valuable human cells, for both drug screening and regenerative medicine. However, processing stem cells is challenging due to the complexity of whole cell products, the number of process parameters, and the typical use of adherent culture. It is hypothesised that a microfabricated adherent culture system could facilitate process development with minimal use of resources. Furthermore, microfl

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27

Atefi, Ehsan. "Aqueous Biphasic 3D Cell Culture Micro-Technology." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692.

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28

Chattopadhyay, Devamita. "Studies on protective additives in cell culture /." The Ohio State University, 1995. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487861796818776.

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29

Ambury, Rachael. "Bioactive sugar surfaces for hepatocyte cell culture." Thesis, University of Manchester, 2010. https://www.research.manchester.ac.uk/portal/en/theses/bioactive-sugar-surfaces-for-hepatocyte-cell-culture(122af33a-35b1-47c1-9579-4568fef47543).html.

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The primary objective of this study was to identify, develop and characterise a novel bioactive surface capable of binding hepatocytes and enabling the retention of hepatocyte-specific cell function during in-vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) found on hepatocytes. Hydrogels were created by modifying a commercially available block co-polymer of polyethylene glycol (PEG) and acrylamide, (PEGA) with galactose moiet

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30

Isomura, Akihiro. "Spatiotemporal pattern formation in cardiac cell culture." 京都大学 (Kyoto University), 2009. http://hdl.handle.net/2433/124393.

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31

Barry, Megan M. Crockett Robert S. "Three-dimensional scaffolds for mammary epithelial cell growth : a thesis /." [San Luis Obispo, Calif. : California Polytechnic State University], 2008. http://digitalcommons.calpoly.edu/theses/12/.

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Thesis (M.S.)--California Polytechnic State University, 2008.<br>Major professor: Robert S. Crockett, Ph.D. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Engineering." "May 2008." Includes bibliographical references (leaves 38-45). Also available on microfiche (1 sheet).

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32

Ouyang, Anli. "Embryonic stem cell culture in fibrous bed bioreactor." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1149001795.

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33

Kittler, Ralf. "Functional genomic analysis of cell cycle progression in human tissue culture cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2006. http://nbn-resolving.de/urn:nbn:de:swb:14-1161253856455-48321.

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The eukaryotic cell cycle orchestrates the precise duplication and distribution of the genetic material, cytoplasm and membranes to daughter cells. In multicellular eukaryotes, cell cycle regulation also governs various organisatorial processes ranging from gametogenesis over multicellular development to tissue formation and repair. Consequently, defects in cell cycle regulation provoke a variety of human cancers. A global view of genes and pathways governing the human cell cycle would advance many research areas and may also deliver novel cancer targets. Therefore this work aimed on the genom

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34

Konagaya, Shuhei. "Design of cell culture substrates for large-scale preparation of neural cells." 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/174963.

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35

Lecault, Véronique. "Microfluidic cell culture arrays for clonal expansion and characterization of mammalian cells." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43667.

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36

Sage, Elizabeth Ann. "The functional competence of animal cells in culture : the NSO cell proteome." Thesis, University of Kent, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399618.

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37

McKilligin, Elaine. "Characterisation and comparison of cell culture models of intimal smooth muscle cells." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624223.

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38

Pasturel, Aurélien. "Tailoring common hydrogels into 3D cell culture templates." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0302.

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L’ingénierie d’hydrogels ; leur structuration et fonctionnalisation à l’échelle cellulaire, est une étape clé pour aboutir à de modèles in-vitro plus physiologiques. À ce jour, elle reste difficile car ces matériaux polymères, mous et riches en eau, sont souvent trop fragiles pour la micro-fabrication traditionnelle. Pour pallier à ce fait, nous avons combinée illumination ultraviolette structurée et chambres de réaction perméables au gaz nous offrant la maitrise sur la distribution de photons, les réactifs et les gaz présents à chaque instant et en tout point d’un champ d’illumination. Nous p

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39

Katalinich, Micheal. "Characterization of Chitosan Films for Cell Culture Applications." Fogler Library, University of Maine, 2001. http://www.library.umaine.edu/theses/pdf/KatalinichM2001.pdf.

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40

Huynh, Minh Diem. "Reduction in apparent stromal cell culture density through transient fusions with osteosarcoma cells." Thesis, The University of Sydney, 2007. http://hdl.handle.net/2123/4675.

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41

Shrader, Carl D. "Insulin-induced endothelial cell proliferation and viability in stretched murine skin and cell culture." Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5260.

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Thesis (Ph. D.)--West Virginia University, 2007.<br>Title from document title page. Document formatted into pages; contains xiii, 127 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.

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42

Najm, Nour Addeen. "In vitro culture studies of tick cell lines." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-146638.

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43

Afshar-Sterle, Shoukat. "Cell, tissue culture and transformation of Triticum tauschii /." Title page, abstract and contents only, 2000. http://web4.library.adelaide.edu.au/theses/09APSP/09apspa258.pdf.

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44

Brown, Stephen Alan. "Cell response to physical stimuli in dynamic culture." Thesis, University of Ulster, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558780.

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Endothelial cells are known to respond directly to their physical environment, with morphological changes in response to fluid shear stress being associated with a number of key cellular functions and disease processes such as angiogenesis, cell migration and atherosclerosis. Alignment and elongation of endothelial cells can result from culturing them on substrates with specific micro-features. If simple, cost-effective physical cues can be used to direct endothelial cell function, significant advances could be made in a number of fields, including Tissue Engineering and Regenerative Medicine

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45

Bailey, Christopher Michael. "Modelling and control of a plant cell culture." Thesis, University of Sheffield, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270201.

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46

Sivathanu, Vivek. "In vitro models for airway epithelial cell culture." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/81726.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2013.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 40-41).<br>This work is about the development of a physiologically relevant model of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear stress, stretch, the air-liquid interface and the biochemical factors in the medium influence the biology of the cells. The aim of this work is to closely approximate conditions in an in v

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Hill, C. J. "Regulating stem cell behaviour using bioengineered culture substrates." Thesis, University of Liverpool, 2017. http://livrepository.liverpool.ac.uk/3006120/.

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Stem cells hold enormous potential for the treatment of injuries and degenerative diseases. In the pursuit of stem cell therapies, a plethora of biomaterials have been developed to induce lineage-specific differentiation or support cell propagation for research and clinical applications. However, the use of stem cells is hindered by the cost of scale-up and risk of zoonotic transmissions from animalderived culture components. Here, we utilise a recombinant protein scaffold composed of self-assembling nanofibres, termed ZT, and assess the systems adaptability for in vitro applications. Protein-

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48

Ge, Cheng. "Novel technologies for cell culture and tissue engineering." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:ab1014cf-80a4-4675-b607-96dc52c39b17.

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Cell culture has been a fundamental tool for the study of cell biology, tissue engineering, stem cell technology and biotechnology in general. It becomes more and more important to have a well-defined physiochemical microenvironment during cell culture. Conventional cell cultures employ expensive, manually controlled incubation equipment, making it difficult to maximize a cultures yield. Furthermore, previous studies use qualitative methods to assess cell culture proliferation that are inherently inaccurate and labour intensive, thereby increasing the cost of production. In addition, three dim

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49

Makene, Vedastus Wilfred. "Cell culture biomarkers for monitoring of wastewater pollutants." University of the Western Cape, 2021. http://hdl.handle.net/11394/8148.

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Philosophiae Doctor - PhD<br>Wastewater is normally composed of a mixture of pollutants. The type and composition of pollutants in a particular wastewater depend on the source of origin. The source and characteristics of a particular wastewater determine the ideal method of sewage treatment. Specific treatment techniques are effective in the removal of certain types of pollutants and may have no impact on the levels of other types of pollutants. Therefore, a combination of treatments and assessment of the quality of effluent before release into the environment is normally recommended. The asse

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Chetty, Avashnee Shamparkesh. "Thermoresponsive 3D scaffolds for non-invasive cell culture." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/25463.

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Conventionally, adherent cells are cultured in vitro using flat 2D cell culture trays. However the 2D cell culture method is tedious, unreliable and does not replicate the complexity of the 3D dynamic environment of native tissue. Nowadays 3D scaffolds can be used to culture cells. However a number of challenges still exist, including the need for destructive enzymes to release confluent cells. Poly(Nisopropylacrylamide) (PNIPAAm), a temperature responsive polymer, has revolutionised the cell culture fraternity by providing a non-invasive means of harvesting adherent cells, whereby confluent c

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