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Journal articles on the topic 'Cell culture'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seekin

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2

Lindee, M. S. "CELL BIOLOGY: The Culture of Cell Culture." Science 316, no. 5831 (2007): 1568–69. http://dx.doi.org/10.1126/science.1142574.

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3

Bauer, Magdalena, Magdalena Metzger, Marvin Corea, Barbara Schädl, Johannes Grillari, and Peter Dungel. "Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture." Life 12, no. 8 (2022): 1216. http://dx.doi.org/10.3390/life12081216.

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In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-ai

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4

First, NL, MM Sims, SP Park, and MJ Kent-First. "Systems for production of calves from cultured bovine embryonic cells." Reproduction, Fertility and Development 6, no. 5 (1994): 553. http://dx.doi.org/10.1071/rd9940553.

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The development of totipotent bovine embryonic cell cultures has great value in cattle breeding. They provide: (1) a mechanism for making large numbers of clonal offspring by nuclear transfer; (2) an efficient gene transfer system through the use of selectable markers to select transgenic cells; and (3) a mechanism for site-specific gene transfer or deletion by homologous DNA sequence recombination. Bovine embryonic cell cultures have been established from blastocyst inner cell mass (ICM) cells, morulae and the precompaction 16-20-cell stage. All have exhibited similar morphology to mouse embr

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5

Finoli, Anthony, Eva Schmelzer, Patrick Over, Ian Nettleship, and Joerg C. Gerlach. "Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/6040146.

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Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support. Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring. Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which

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6

Levine, J. F., and F. E. Stockdale. "Cell-cell interactions promote mammary epithelial cell differentiation." Journal of Cell Biology 100, no. 5 (1985): 1415–22. http://dx.doi.org/10.1083/jcb.100.5.1415.

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Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. Insofar as caseins are specific molecular markers of mammary epithelial differentiation, rat anti-mouse casein monoclonal antibodies were raised against the three major mouse casein components to study this interaction. Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1

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7

Kakigi, Akinobu. "Cell Culture." Equilibrium Research 67, no. 1 (2008): 1–5. http://dx.doi.org/10.3757/jser.67.1.

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8

Ponec, Maria, and Esther Boelsma. "Cell Culture." American Journal of Contact Dermatitis 8, no. 2 (1997): 100–102. http://dx.doi.org/10.1097/01634989-199706000-00021.

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9

Ponec, Maria, and Esther Boelsma. "Cell Culture." Dermatitis 8, no. 2 (1997): 100–102. http://dx.doi.org/10.1097/01206501-199706000-00021.

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10

DeGaspari, John. "Cell Culture." Mechanical Engineering 123, no. 03 (2001): 56–59. http://dx.doi.org/10.1115/1.2001-mar-1.

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This article focuses on Tribon Bearing Co. plant in Brook Park, OH, a manufacturer of discrete carbon composite parts and shapes that had been plagued by problems that threatened its existence. The old Tribon plant was a traditional manufacturing setup, in which operations were highly compartmentalized. Equipment was arranged according to purpose and job functions were narrowly defined. The plant’s production control manager, there was plenty of distrust and bad feelings between front-line management and the plant floor workforce. Workcell leaders work with manufacturing engineers to develop a

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11

Boulton, Mike. "Cell culture." Eye 4, no. 4 (1990): 622–31. http://dx.doi.org/10.1038/eye.1990.87.

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12

Donaldson, CD, and KN Bishop. "Cell culture." British Journal of Hospital Medicine 76, no. 1 (2015): C2—C5. http://dx.doi.org/10.12968/hmed.2015.76.1.c2.

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13

Koh, Benson, Nadiah Sulaiman, Mh Busra Fauzi, et al. "A Three-Dimensional Xeno-Free Culture Condition for Wharton’s Jelly-Mesenchymal Stem Cells: The Pros and Cons." International Journal of Molecular Sciences 24, no. 4 (2023): 3745. http://dx.doi.org/10.3390/ijms24043745.

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Xeno-free three-dimensional cultures are gaining attention for mesenchymal stem cell (MSCs) expansion in clinical applications. We investigated the potential of xeno-free serum alternatives, human serum and human platelet lysate, to replace the current conventional use of foetal bovine serum for subsequent MSCs microcarrier cultures. In this study, Wharton’s Jelly MSCs were cultured in nine different media combinations to identify the best xeno-free culture media for MSCs culture. Cell proliferation and viability were identified, and the cultured MSCs were characterised in accordance with the

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14

Yoshino, T. P., U. Bickham, and C. J. Bayne. "Molluscan cells in culture: primary cell cultures and cell lines." Canadian Journal of Zoology 91, no. 6 (2013): 391–404. http://dx.doi.org/10.1139/cjz-2012-0258.

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of

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15

Wiszniewska, A., and B. Piwowarczyk. "Studies on cell wall regeneration in protoplast culture of legumes – the effect of organic medium additives on cell wall components." Czech Journal of Genetics and Plant Breeding 50, No. 2 (2014): 84–91. http://dx.doi.org/10.17221/108/2013-cjgpb.

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The cell wall regeneration in mesophyll protoplasts of yellow lupin and grass pea was studied. The occurrence of cell wall components: cellulose, callose and arabinogalactan proteins was analysed during 15 days of culture. Protoplasts were cultured in different media to test the effect of culture environment on the cell wall regeneration. Medium supplementation with 2 mg/l chitosan resulted in prolonged viability, more balanced cellulose resynthesis, increased callose formation and induction of mitotic divisions in protoplast-derived cells of both examined legumes. In chitosan-enriched medium

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16

Hegazy, Ragia M., Eman Farouk, and Taghreed G. Kharboush. "Silver Nitrate Particlesnanotoxicity using Cell Culture and Apoptosis (Genetic and Cell Study)." Indian Journal of Applied Research 4, no. 6 (2011): 1–8. http://dx.doi.org/10.15373/2249555x/june2014/184.

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17

Ceresa, Claudia C., Alan J. Knox, and Simon R. Johnson. "Use of a three-dimensional cell culture model to study airway smooth muscle-mast cell interactions in airway remodeling." American Journal of Physiology-Lung Cellular and Molecular Physiology 296, no. 6 (2009): L1059—L1066. http://dx.doi.org/10.1152/ajplung.90445.2008.

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Increased airway smooth muscle (ASM) mass and infiltration by mast cells are key features of airway remodeling in asthma. We describe a model to investigate the relationship between ASM, the extracellular matrix, mast cells, and airway remodeling. ASM cells were cultured in a three-dimensional (3-D) collagen I gel (3-D culture) alone or with mast cells. Immunocytochemistry and Western blotting of ASM in 3-D cultures revealed a spindle-shaped morphology and significantly lower α-smooth muscle actin and vimentin expression than in ASM cultured in monolayers on collagen type I or plastic (2-D cul

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18

Celkova, Patricia, Emilie Seydoux, Susan De Groof, and Loretta Müller. "Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures." Life 15, no. 6 (2025): 958. https://doi.org/10.3390/life15060958.

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Cultures of primary human nasal epithelial cells (hNECs) differentiated at the air–liquid interface (ALI) represent a sophisticated and widely used model of the human upper respiratory epithelium. Despite the availability of various cell culture insert types and the well-established understanding that different culture media influence the cell culture characteristics, the possible impact of the insert brand remains rather underexplored. We cultured hNECs from nineteen healthy adult donors on three distinct brands of commercially available inserts—Corning® Transwell®, CELLTREAT®, and ThinCert®—

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19

McAdams, Todd A., William M. Miller, and E. Terry Papoutsakis. "Hematopoietic cell culture therapies (Part I): cell culture considerations." Trends in Biotechnology 14, no. 9 (1996): 341–49. http://dx.doi.org/10.1016/0167-7799(96)10047-0.

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20

Srishti, Gupta1 Andleeb Arif1 Rajeshwar Khandare2 Kartikey Rastogi3. "A brief focus on cell culture and its techniques." Science World a Monthly e Magazine 3, no. 6 (2023): 1163–68. https://doi.org/10.5281/zenodo.8082134.

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Cell culture involves a complex of processes of cell isolation from their natural environment (<em>in vivo</em>) and subsequent growth in a controlled environmental artificial condition (<em>in vitro</em>). Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic viral isolation depends on the availability of permissible cell cultures. Cell culture provides the required setting for the detection and identification of numerous pathogens of human

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21

Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.958.

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Abstract Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and l

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22

Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.bloodjournal863958.

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Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and long-term

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23

Orlidge, A., and P. A. D'Amore. "Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells." Journal of Cell Biology 105, no. 3 (1987): 1455–62. http://dx.doi.org/10.1083/jcb.105.3.1455.

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Morphological studies of developing capillaries and observations of alterations in capillaries associated with pathologic neovascularization indicate that pericytes may act as suppressors of endothelial cell (EC) growth. We have developed systems that enable us to investigate this possibility in vitro. Two models were used: a co-culture system that allowed direct contact between pericytes and ECs and a co-culture system that prevented physical contact but allowed diffusion of soluble factors. For these studies, co-cultures were established between bovine capillary ECs and the following growth-

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24

Gassl, Vincent, Merel R. Aberle, Bas Boonen, Rianne D. W. Vaes, Steven W. M. Olde Damink, and Sander S. Rensen. "Chemosensitivity of 3D Pancreatic Cancer Organoids Is Not Affected by Transformation to 2D Culture or Switch to Physiological Culture Medium." Cancers 14, no. 22 (2022): 5617. http://dx.doi.org/10.3390/cancers14225617.

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Organoids are increasingly used to investigate patient-specific drug responsiveness, but organoid culture is complex and expensive, and carried out in rich, non-physiological media. We investigated reproducibility of drug-responsiveness of primary cell cultures in 2D versus 3D and in conventional versus physiological cell culture medium. 3D pancreatic ductal adenocarcinoma organoid cultures PANCO09b and PANCO11b were converted to primary cell cultures growing in 2D. Transformed 2D cultures were grown in physiological Plasmax medium or Advanced-DMEM/F12. Sensitivity towards gemcitabine, paclita

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25

Bishimbayeva, N. K., I. A. Sartbayeva, A. S. Murtazina та E. A. Gunter. "Сhemical composition of polysaccharides from wheat cell culture". International Journal of Biology and Chemistry 8, № 2 (2015): 13–17. http://dx.doi.org/10.26577/2218-7979-2015-8-2-13-17.

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26

Tynykulov, Marat Korganbekovich. "Peculiarity of currant and perpetual repair cell culture." Bulletin of the Karaganda University. “Biology, medicine, geography Series” 108, no. 4 (2022): 140–47. http://dx.doi.org/10.31489/2022bmg4/140-147.

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Perpetual raspberry is a group of raspberry varieties distinguished by their ability to bear fruit on two-year and one-year stem shoots. Black currant (Ríbes nígrum) is a deciduous shrub, family Gooseberry (Grossulariaceae), related to currants (Ribes). The article presents methods of cell selection of perpetual raspberries and currants. Crop varieties resistant to extreme natural factors, obtaining high harvest, and two harvests per year were considered. The chemical composition of the used nutrient medium, the content of micro- and macroelements, and organic substances were taken into accoun

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27

Vieira, João Paulo J., Ilva F. Souza, Marcelo B. Pedras, Danilo B. Oliveira, Libardo A. González-Torres, and Bethânia A. Avelar-Freitas. "Morphology of Adherent Cells of the Line Vero Cultivated in a Three-Dimensional Environment inside a Microfluidic Device Differs from their Morphology when Cultivated in Monolayers." Journal of Advances in Medicine and Medical Research 36, no. 8 (2024): 230–37. http://dx.doi.org/10.9734/jammr/2024/v36i85542.

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Traditional in vitro culture models have significant limitations in mimicking important physiological interactions, such as cell-cell interactions, cell-extracellular matrix interactions, and the three-dimensional morphology of cells. In contrast, 3D culture models have the ability to replicate the natural three-dimensional environment of cells. Aims: The objective of this study is to evaluate the morphology of adherent Vero cells grown in two-dimensional (2D) and three-dimensional (3D) culture models. Methodology: For the 2D culture model, Vero cells were thawed and grown in culture flasks in

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28

Contramaestre, A. P., F. Sifontes, R. Marín, and M. I. Camejo. "Secretion of stem cell factor and granulocyte–macrophage colony-stimulating factor by mouse embryos in culture: influence of group culture." Zygote 16, no. 4 (2008): 297–301. http://dx.doi.org/10.1017/s0967199408004760.

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SummaryPrevious studies showed that the addition of a growth factor to the culture medium could modulate embryo development. The possible secretion of different factors to the culture medium by the embryo itself, however, has been poorly evaluated. The present study was designed to investigate: (1) the influence of single or group culture on the development of 2-cell mouse embryos (strain CD-1) to the blastocyst stage; (2) the release of granulocyte–macrophage colony-stimulating factor (GM-CSF) and stem cell factor (SCF) into the culture medium by the embryo; and (3) the levels of GM-CSF and S

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29

Eglen, Richard M., and Terry Reisine. "Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 2: Spheroids, Organoids, and Disease Modeling." SLAS TECHNOLOGY: Translating Life Sciences Innovation 24, no. 1 (2019): 18–27. http://dx.doi.org/10.1177/2472630318803275.

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Human induced pluripotent stem cells (HiPSCs) provide several advantages for drug discovery, but principally they provide a source of clinically relevant tissue. Furthermore, the use of HiPSCs cultured in three-dimensional (3D) systems, as opposed to traditional two-dimensional (2D) culture approaches, better represents the complex tissue architecture in vivo. The use of HiPSCs in 3D spheroid and organoid culture is now growing, but particularly when using myocardial, intestinal enteric nervous system, and retinal cell lines. However, organoid cell culture is perhaps making the most notable im

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30

Wessel, Gary M. "The sub-culture of cell culture." Molecular Reproduction and Development 78, no. 2 (2011): Fm i. http://dx.doi.org/10.1002/mrd.21292.

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31

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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32

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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33

Goto, K., N. Iwai, K. Ide, Y. Takuma, and Y. Nakanishi. "Viability of one-cell bovine embryos cultured in vitro: comparison of cell-free culture with co-culture." Reproduction 100, no. 1 (1994): 239–43. http://dx.doi.org/10.1530/jrf.0.1000239.

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34

Kociubiński, Andrzej. "Electric cell-substrate impedance sensing in biocompatibility research." Journal of Electrical Bioimpedance 12, no. 1 (2021): 163–68. http://dx.doi.org/10.2478/joeb-2021-0019.

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Abstract In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the comple

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35

He, Lingjie, Cheng Zhao, Qi Xiao, et al. "Profiling the Physiological Roles in Fish Primary Cell Culture." Biology 12, no. 12 (2023): 1454. http://dx.doi.org/10.3390/biology12121454.

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Fish primary cell culture has emerged as a valuable tool for investigating the physiological roles and responses of various cell types found in fish species. This review aims to provide an overview of the advancements and applications of fish primary cell culture techniques, focusing on the profiling of physiological roles exhibited by fish cells in vitro. Fish primary cell culture involves the isolation and cultivation of cells directly derived from fish tissues, maintaining their functional characteristics and enabling researchers to study their behavior and responses under controlled condit

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36

Schick, Bernhard, Gregor Wolf, Bernd F. M. Romeike, Pedro Mestres, Mark Praetorius, and Peter K. Plinkert. "Dural Cell Culture." Cells Tissues Organs 173, no. 3 (2003): 129–37. http://dx.doi.org/10.1159/000069469.

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37

Kawamura, Kenji. "Cell Culture Surface." MEMBRANE 30, no. 3 (2005): 171–73. http://dx.doi.org/10.5360/membrane.30.171.

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38

Gershon, Diane. "Cell culture supplement." Nature 352, no. 6338 (1991): 829–32. http://dx.doi.org/10.1038/352829a0.

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39

O'Brien, S. J. "Cell culture forensics." Proceedings of the National Academy of Sciences 98, no. 14 (2001): 7656–58. http://dx.doi.org/10.1073/pnas.141237598.

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40

Shargool, PD. "Plant cell culture." Biochemical Education 15, no. 1 (1987): 51. http://dx.doi.org/10.1016/0307-4412(87)90167-1.

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Harris, Ian. "Animal cell culture." Biochemical Education 21, no. 4 (1993): 226. http://dx.doi.org/10.1016/0307-4412(93)90121-f.

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Hu, Wei-Shou. "Cell culture engineering." Trends in Biotechnology 6, no. 5 (1988): 83–84. http://dx.doi.org/10.1016/0167-7799(88)90061-3.

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KISS, R. "Cell culture engineering." Trends in Biotechnology 14, no. 6 (1996): 179–81. http://dx.doi.org/10.1016/0167-7799(96)30010-3.

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44

Scragg, A. H. "Plant cell culture." Journal of Biotechnology 26, no. 1 (1992): vii. http://dx.doi.org/10.1016/0168-1656(92)90066-i.

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45

Mazie, J. C. "Animal cell culture." Biochimie 72, no. 12 (1990): 898. http://dx.doi.org/10.1016/0300-9084(90)90012-6.

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46

Jackson, Chris. "Endothelial cell culture." Trends in Cell Biology 8, no. 3 (1998): 130–31. http://dx.doi.org/10.1016/s0962-8924(98)01235-5.

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47

Hall, P. "Animal Cell Culture." Journal of Clinical Pathology 43, no. 9 (1990): 785. http://dx.doi.org/10.1136/jcp.43.9.785-a.

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48

Shephard, Elizabeth A. "Animal cell culture." Endeavour 17, no. 4 (1993): 202. http://dx.doi.org/10.1016/0160-9327(93)90076-f.

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49

Nilsson, Kjell. "Microcarrier Cell Culture." Biotechnology and Genetic Engineering Reviews 6, no. 1 (1988): 404–39. http://dx.doi.org/10.1080/02648725.1988.10647854.

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50

Mehling, Matthias, and Savaş Tay. "Microfluidic cell culture." Current Opinion in Biotechnology 25 (February 2014): 95–102. http://dx.doi.org/10.1016/j.copbio.2013.10.005.

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