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Relevant bibliographies by topics / Chemical engineering. Microbiology / Dissertations / Theses

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Dissertations / Theses on the topic 'Chemical engineering. Microbiology'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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1

Mansfield, Robert Patrick William. "Developments in genetic engineering of novel acetogens." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/51833/.

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The development of processes for sustainable energy and chemical production is of great importance for the health of our planet. Utilising suitable feedstocks such as renewable resources and existing waste streams is central to making such processes a reality. Microorganisms can be employed in the processing of a diverse range of such feedstocks, offering unique routes of production for useful and valuable chemical products. Acetogenic organisms, capable of fermenting single carbon (C1) feedstocks are especially interesting from the perspective of industrial application. Their natural metaboli

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2

Kongsaeree, Puapong Kelly Christine. "Optimization of recombinant ligninolytic enzyme production in Pichia pastoris." Related electronic resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2004. http://wwwlib.umi.com/cr/syr/main.

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3

Hyndman, Caroline Louise. "Immobilization of lactococcus lactis subsp. cremoris within cross- linked protein microcapsules." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22443.

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Lactococcus lactis subspecies cremoris was encapsulated by cross-linking emulsified gelatin at an oil/water interface. The organic phase in the emulsion step consisted of nontoxic vegetable or silicone oil and the exposure of the cells to cross-linking agent was minimized in two ways. The concentration of tolylene-2,4-diisocyanate was reduced to 34 mM and a lower viscosity oil was used as the continuous phase in the emulsion step so as to decrease the specific surface area of the microcapsules. Viable cell count (cfu/ml milk) was estimated to be reduced by five orders of magnitude for microcap

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4

May, Michael 1972. "Production of Lipase by Candida bombicola in a self-cycling fermenter (SCF)." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27242.

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The Self-Cycling Fermentation (SCF) technique and an agar diffusion assay were used to follow the production of lipase by Candida bombicola. Repeatable lipase production profiles were determined for two different media. Each medium gave a considerably different profile.<br>When the broth contained oil, 60 to 80% of the lipase activity remained after freezing. All activity was lost when the broth did not contain oil.<br>The lipase was found to be associated with the cell. Up to a certain concentration of lipase, the presence of agar caused the lipase to disassociate from the cell and diffuse in

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5

Helle, Steve. "Biosurfactants & cellulose hydrolysis." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61308.

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The action of many antimicrobial agents is dependent on their ability to interact with biological membranes. A group of polypeptide antibiotics was found to have surface activite properties. One of them, gramicidinS, produced a minimum in the surface tension curve, which was attributed to instabilities in the intra-molecular hydrogen bonds. Biosurfactants were found to have a great effect on the two phase hydrolysis of cellulose by cellulase. Seven times as much sugar was produced by the hydrolysis of Sigmacell 100 when the biosurfactant sophorolipid was present. The surfactant affects the ads

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6

Quesnel, David M. (David Marc). "Hydrocarbon utilization by Corynebacterium dioxydans." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23373.

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Batch fermentations of Corynebacterium dioxydans were performed using aliphatic hydrocarbons as substrates. The production of carboxylic acids was monitored throughout these fermentations. A relationship between the carbon chain length of the hydrocarbon substrate and the carboxylic acids was observed. The maximum yield of carboxylic acid was found to occur in the exponential growth phase, with the initial complete medium. Cell hydrophobicity is the proposed hypothesis for the residual hydrocarbon found in these fermentations. The addition of synthetic surfactant increased the yield of carboxy

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7

Müller, Stefan 1971. "Biodegradation of polyethylene thermolysis residue." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20509.

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The ability of several microorganisms to degrade polyethylene thermolysis residue was investigated. This residue is mainly comprised of n-alkanes and n-1-alkenes ranging in chain length of 15 to 45 carbon units and therefore resembles crude oil. The microorganisms investigated were: Y. lipolytica ATCC 8662, Y. lipolytica ATCC 20460, A. paraffineus ATCC 19558, P. aeruginosa PAO1, P. putida ATCC 12633, R. rhodochrous ATCC 21766 and Rhodococcus sp. ATCC 29671. It was found that all seven microorganisms tested were able to degrade the polyethylene thermolysis residue. Results suggest that R. rhodo

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8

Drouin, Carole M. "Partition of biosurfactants in two-phase fermentation media." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61246.

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The partition of surfactants and of a biosurfactant-producing microorganism was studied in polyethylene glycol and dextran aqueous two-phase systems. In the presence of sodium phosphate, surfactants distributed themselves according to their charge. Cationic surfactants preferred the bottom phase, while anionic surfactants were attracted to the top phase. Increasing the phosphate molarity, the pH, the polymer concentration or molecular weight all resulted in a more one-sided surfactant partitioning. Biosurfactant partition was weaker than synthetic surfactant partition due to their weaker effec

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9

Atiyeh, Hasan K. "Study of the production of fructose and ethanol from sucrose and molasses media using Saccharomyces cerevisiae ATCC 36858." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/28948.

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Fructose is available as a component of invert sugar and of high fructose corn syrups (HFCS) in which fructose and glucose are present in nearly equimolar concentrations. This study reports on a new mutant, Saccharomyces cerevisiae ATCC 36858, which is able to produce enriched and pure fructose syrups from synthetic glucose/fructose mixtures, sucrose and raffinose, and from molasses media. In this study, the results showed that S. cerevisiae ATCC 36858 can hydrolyze sucrose, raffinose and melibiose then selectively ferment glucose and galactose to ethanol. In batch tests, the fructose yield w

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10

Zhou, Yi J. (Yi Jenny). "Immobilization of lactic acid bacteria in alginate : spectrophotometric measurements of intracapsular cell population and control of cell release by chitosan coating." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22686.

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Lactococcus lactis subspecies cremoris was entrapped within calcium alginate beads. A spectrophotometric method to estimate the immobilized cell population was developed involving the measurement of the absorbance of whole beads using cell-free beads as reference or the absorbance of cells released after bead liquefaction.<br>Cell release from the beads during milk fermentation was studied. Coating the beads with low viscosity chitosan reduced cell release from 10$ sp5$ CFU/ml to 10$ sp3$ CFU/ml after 6 hours fermentation with initial intracapsular cell population of 10$ sp8$ CFU/ml medium. Co

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11

Nalli, Sandro. "Biological degradation of plasticizers and their metabolites." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85630.

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Plasticizers are widely used as additives for the production of PVC and other types of plastics. They have been observed to leach out of the solid matrix over the course of the lifetime of the finished product. These compounds have now been observed across the globe and in different environments. The main focus of this work is the study of the interactions of industrial plasticizers with soil microorganisms including bacteria, yeast and fungi.<br>This research is concerned with the microbial degradation of plasticizers such as di-2-ethylhexyl phthalate (DEHP) and di-2-ethylhexyl adipate

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12

Li, Qian. "Production of Carbohydrases for Developing Soy Meal as Protein Source for AnimalFeed." University of Akron / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron1490572358360331.

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13

Ammar, Ehab Mohamed. "Metabolic Engineering of Propionibacteria for Enhanced Propionic Acid and n-Propanol Fermentative Production." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374174278.

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14

Quiroga, Blas III. "Optimization of Protein Expression of an ELP-GFP Fusion Protein In Bacterial Systems." Cleveland State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611791577229689.

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15

Kijkla, Pruch. "Biocide Mitigation of Carbon Steel and Stainless Steel Biocorrosion by Pure-Strain and Mixed-Culture Microbial Biofilms." Ohio University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1619007982435067.

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16

Andlay, Gunjan. "Commercialization of Anaerobic Contact Process for Anaerobic Digestion of Algae." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1270240284.

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17

Sendamangalam, Varunraj. "The Influence of Biofilm Structure and Total Interaction Energy on Pathogen Retention by Biofilm." University of Toledo / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1340381730.

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18

Shao, Heng. "Efficient Production of Plat-form from Organic Acids from Ligocellulosic and Algal Biomass Carbohydrates." University of Toledo / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1420847319.

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19

Coffman, Anthony M. "Production of Carbohydrases by Fungus Trichoderma Reesei Grown on Soy-based Media." University of Akron / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1381761363.

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20

Sanchez, Alamina Arcelia del Carmen. "The Evaluation of Ferrous, Ferric and an Iron Oxidizing Bacterium (Acidithiobacillus ferrooxidans) on the Corrosion of Stainless Steel 304L." University of Akron / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron1477046516998666.

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21

Grant, Marcus T. "Biomimetic Production Techniques for Mechanical and Chemical Characterization of Sucker Ring Teeth Isoform-12 From the Dosidicus Gigas Squid." Wright State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484614367176197.

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22

Pham, Errek Manh Trung. "Producing A Peptide For Use In A Blood Biosensor For Injury Detection." Youngstown State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1607519672342672.

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23

Probst, Kyle V. "Single cell oil production using Lipomyces starkeyi : fermentation, lipid analysis and use of renewable hemicellulose-rich feedstocks." Diss., Kansas State University, 2014. http://hdl.handle.net/2097/18225.

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Doctor of Philosophy<br>Department of Grain Science and Industry<br>Praveen V. Vadlani<br>As the world population continues to grow and the uncertainty of petroleum and food availability transpires, alternative resources will be needed to meet our demands. Single cell oil (SCO) from oleaginous yeast is a renewable noncrop-based resource that can be used for the production of petroleum counterparts. Currently, commercial production is limited, mainly due to high production costs and competition from cheaper alternatives. As a result, improved fermentation techniques, utilization of low-valued f

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24

Roberts, Alexander Colin. "Production and Harvest of Microalgae in Wastewater Raceways with Resource Recycling." DigitalCommons@CalPoly, 2015. https://digitalcommons.calpoly.edu/theses/1537.

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Microalgae can be grown on municipal wastewater media to both treat the wastewater and produce feedstock for algae biofuel production. However the reliability of treatment must be demonstrated, as well as high areal algae productivity on recycled wastewater media and efficient sedimentation harvesting. This processes was studied at pilot scale in the present research. A pilot facility was operated with nine CO2-supplemented raceway ponds, each with a 33-m2 surface area and a 0.3-m depth, continuously from March 6, 2013 through September 24, 2014. The ponds were operated as three sets of tripli

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25

Dehghan, Abnavi Mohammadreza Dehghan. "CHLORINE DECAY AND PATHOGEN CROSS CONTAMINATION DYNAMICS IN FRESH PRODUCE WASHING PROCESS." Cleveland State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=csu1624196282479244.

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26

Nasr, Mostafa M. "Mitigation of Microbially Induced Concrete Corrosion in Wastewater Infrastructure using Surface Treatments." Youngstown State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1619950217448915.

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27

Chen, Yajie. "Microbiologically influenced corrosion of carbon steel caused by a sulfate reducing bacterium." University of Akron / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=akron1468357213.

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28

Bourquin, Yannyk Parulian Julian. "Shaping surface waves for diagnostics." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/4167/.

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Infectious diseases continue to kill millions of people every year and are a significant burden on the socio-economic development of developing countries. After many years of international policy aimed at containing diseases, it has recently become an explicit aim to move towards elimination of infectious diseases. However, if this is to occur, it will be necessary to have highly eficacious diagnostic tools to ensure infected individuals are identified and treated. However, the diagnosis of infectious diseases in the developed and developing world requires the full integration of complex assay

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29

Zhang, Kun. "Fumaric Acid Fermentation by Rhizopus oryzae with Integrated Separation Technologies." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354729467.

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30

Jia, Ru. "Mechanisms of Microbiologically Influenced Corrosion Caused by Corrosive Biofilms and its Mitigation Using Enhanced Biocide Treatment." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1541425677541433.

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31

Rismani-Yazdi, Hamid. "Bioconversion of cellulose into electrical energy in microbial fuel cells." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1211313869.

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32

Evans, Morgan Volker. "Microbial transformations of organic chemicals in produced fluid from hydraulically fractured natural-gas wells." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555609276432456.

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33

bhardwaj, vinay. "Label-free surface-enhanced Raman spectroscopy-linked immunosensor assay (SLISA) for environmental surveillance." FIU Digital Commons, 2015. http://digitalcommons.fiu.edu/etd/2321.

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The contamination of the environment, accidental or intentional, in particular with chemical toxins such as industrial chemicals and chemical warfare agents has increased public fear. There is a critical requirement for the continuous detection of toxins present at very low levels in the environment. Indeed, some ultra-sensitive analytical techniques already exist, for example chromatography and mass spectroscopy, which are approved by the US Environmental Protection Agency for the detection of toxins. However, these techniques are limited to the detection of known toxins. Cellular expression

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34

Gardel, Emily Jeanette. "Microbe-electrode interactions: The chemico-physical environment and electron transfer." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11185.

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This thesis presents studies that examine microbial extracellular electron transfer that an emphasis characterizing how environmental conditions influence electron flux between microbes and a solid-phase electron donor or acceptor. I used bioelectrochemical systems (BESs), fluorescence and electron microscopy, chemical measurements, 16S rRNA analysis, and qRT-PCR to study these relationships among chemical, physical and biological parameters and processes.<br>Engineering and Applied Sciences

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35

Bidaud, Christine. "Biodégradation des hydrocarbures aromatiques polycycliques. Approche microbiologique et application au traitement d'un sol pollué." Phd thesis, Ecole Nationale Supérieure des Mines de Saint-Etienne, 1998. http://tel.archives-ouvertes.fr/tel-00841329.

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Les Hydrocarbures Aromatiques Polycycliques (HAP) sont des molécules qui posent un grave problème environnemental à cause de leurs propriétés toxiques, cancérigènes, voire tératogènes. Les sites d'anciennes usines à gaz sont souvent pollués par ces produits issus de la combustion incomplète de matières organiques. Notre travail a été réalisé à partir d'échantillons de sol issus d'un site d'ancienne usine à gaz. Nous avons d'abord étudié la biodégradabilité naturelle des 16 HAP ciblés par l'U.S. E.P.A. à partir d'échantillons de sol prélevés sur le site. Pour cela, nous avons étudié leur biodég

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36

Lin, Henry. "Metabolic network design and engineering in Escherichia coli." Thesis, 2005. http://hdl.handle.net/1911/18854.

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This thesis is a study of metabolic engineering to design and engineer novel metabolic networks with improved metabolic processes that can increase product yield and enhance cellular properties. The succinate synthesis pathways in E. coli were chosen as the model system for network design and optimization. The ultimate goal is to create succinate production systems in E. coli that not only achieve the maximum theoretical yield of succinate, but also are highly efficient and robust. Different carbon sources with different oxidation states and transport systems, such as sorbitol and xylose, were

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37

Yang, Yea-Tyng. "Metabolic engineering and flux analysis of Escherichia coli." Thesis, 1999. http://hdl.handle.net/1911/19454.

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The advances in molecular biology, genome sequencing and mathematical tools have led to growing interest in metabolic engineering, the goal of which is to redirect metabolic fluxes for industrial and medical purposes. Escherichia coli was chosen as the model system for several reasons: the ease of genetic manipulation, the wealth of genetic information, its capacity for rapid growth, and the availability of inexpensive and standardized cultivation protocols. The effects of precise perturbation in the central metabolic pathway of E. coli with an emphasis on the competition at the pyruvate node

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38

"Electrochemical Charaterization of Anode-respiring Geobacter sulfurreducens and Geoalkalibacter subterraneus." Master's thesis, 2013. http://hdl.handle.net/2286/R.I.18671.

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abstract: To further the efforts producing energy from more renewable sources, microbial electrochemical cells (MXCs) can utilize anode respiring bacteria (ARB) to couple the oxidation of an organic substrate to the delivery of electrons to the anode. Although ARB such as Geobacter and Shewanella have been well-studied in terms of their microbiology and electrochemistry, much is still unknown about the mechanism of electron transfer to the anode. To this end, this thesis seeks to elucidate the complexities of electron transfer existing in Geobacter sulfurreducens biofilms by employing Electroc

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39

Cardenas-Gonzalez, Beatriz. "Characterization of compost biofilter media." 1998. https://scholarworks.umass.edu/dissertations/AAI9841846.

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The goal of this study was to determine the physical, chemical and biological properties that can be used to determine the performance of compost as biofilter media. Four types of compost were characterized with regard to pH, total organic carbon and nitrogen content in solid matrix and water extracts, moisture content, oxygen uptake rates, and microbial population densities. Compost were blended with ceramic pellets and oyster shell into three media mixtures: biosolids (BS), yard waste (YW) and a biosolids/horse manure mixture (BSHM). Performance was determined in laboratory scale systems tre

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40

Chhiber, Aasheesh. "Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli." Thesis, 2001. http://hdl.handle.net/1911/17408.

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Using biochemical engineering principles and metabolic engineering tools in tandem can considerably improve process productivity by manipulation of the throughput of certain pathways. Escherichia coli was chosen as the model system because of its vast available genetic information, rapid growth and the practice of standardized experimental protocols. The pathway leading to the production of succinic acid was examined for the effects of level of carbon substrate available to the system and overexpression of the phosphoenolpyruvate carboxylase (PEPC) enzyme system coupled with pathway manipulati

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41

Murarka, Abhishek. "Metabolic flux analysis of fermentative carbon metabolism in Escherichia coli." Thesis, 2008. http://hdl.handle.net/1911/22280.

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Recently, the production of various chemicals and fuels via microbial fermentation has gained momentum. Development of efficient bio-processes requires a system-scale understanding of the metabolic network of biocatalysts. In this context, we evaluated the roles of metabolic pathways and enzymes during fermentation of various substrates in E. coli using metabolic flux analysis (MFA), which is a powerful and efficient tool for comprehensive investigation of a biological system. The combination of substrates studied covered the full range of oxidation states of common carbon sources. During ferm

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42

Niranjan, S. C. "Sensitivity analysis of a mechanistic growth model of Escherichia coli." Thesis, 1991. http://hdl.handle.net/1911/16521.

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A detailed mechanistic model, with a large number of metabolites and parameters, describing growth of a single cell of the bacterium Escherichia coli is simulated and the resulting predictions are compared with available reported experimental observations. The effects of small time-invariant perturbations in each of the parameters on final model predictions of metabolite concentrations and doubling times are systematically investigated using the theory of sensitivity analysis. This is quantified by defining (linear) sensitivity coefficients $\underline{\Lambda},$ and are subsequently used to i

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43

Hanly, Timothy J. "Dynamic modeling of synthetic microbial consortia to optimize the co-fermentation of glucose and xylose." 2013. https://scholarworks.umass.edu/dissertations/AAI3603093.

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Second-generation biofuels have the potential to replace fossil fuels in the energy economy without negatively impacting the food supply. An effective biocatalyst must be able to convert all sugars found in lignocellulosic hydrolysates to biofuels. Few microbes exist in that have both a wide substrate range and high ethanol yields necessary for this process. Mixed culture biotechnology is a promising alternative to the use of single organisms in the production of fuels from lignocellulosic biomass. These systems mimic natural processes for the degradation of lignocellulose and exploit the nati

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Aristidou, Aristos Andrea. "Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli." Thesis, 1995. http://hdl.handle.net/1911/16799.

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This study focuses on the application of metabolic engineering techniques to diminish acetate excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity under the control of the pH-regulated promoter. This recently developed expression system has the potential for very high gene expression, of 30 to 40% of the total soluble cell protein. Three approaches are presented. E. coli acetate mutants were isolated and characterized as recombinant hosts in rel

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"Engineering Escherichia coli for the Novel and Enhanced Biosynthesis of Phenol, Catechol, and Muconic Acid." Doctoral diss., 2017. http://hdl.handle.net/2286/R.I.45578.

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abstract: The engineering of microbial cell factories capable of synthesizing industrially relevant chemical building blocks is an attractive alternative to conventional petrochemical-based production methods. This work focuses on the novel and enhanced biosynthesis of phenol, catechol, and muconic acid (MA). Although the complete biosynthesis from glucose has been previously demonstrated for all three compounds, established production routes suffer from notable inherent limitations. Here, multiple pathways to the same three products were engineered, each incorporating unique enzyme chemistrie

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46

Khan, Tariq A. "Anaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum." Thesis, 1996. http://hdl.handle.net/1911/14073.

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In this work, rapid TNT ($>$150 mg/L) transformation by Clostridium acetobutylicum via a reductive pathway to monoaminonitrotoluenes, diaminonitrotoluenes, and on to undetectable end products was observed. The maximum velocities were greatest for TNT reduction, followed by 2A46DNT, 4A26DNT, and 24DA6NT. Initial reduction was preferred at the para position. The measured TNT transformation rates were considerably higher than those observed by others. The transformation of hydroxylamino intermediates was rate limiting. Rates and extents of transformation of TNT and its metabolites were affected b

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47

Yu, Peng. "Characterization and application of a periplasmic protein releasing system for extracellular recombinant protein production in Escherichia coli." Thesis, 1993. http://hdl.handle.net/1911/16694.

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Escherichia coli is commonly used as a host in the production of high value therapeutic proteins. However, one major drawback associated with E. coli being a host is the intracellular location of product proteins. As a result, mechanical cell disruption is commonly employed in biotechnology industry for protein release. Unfortunately, mechanical disintegration always contaminates the target protein with other cellular substances. On the other hand, progress in the study of protein translocation across membrane often allows the transport of proteins from the cytoplasm to the periplasm. However,

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Pucik, Lara Ellen. "The behavior of TNT in model chemical redox systems and the fate of TNT reduction products in aerobic microbial systems." Thesis, 1996. http://hdl.handle.net/1911/14075.

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When exposed to the Fenton oxidation process, approximately 25% of the initial TNT was mineralized and approximately 63% was converted to unknown soluble products. When exposed to Fe(0) under acidic conditions, TNT was completely reduced to unknown products, approximately 20% of which were not soluble. Reduction of TNT by Fe(0) did not occur above pH 3.0. When exposed to sulfide, TNT was reduced to a mixture of 4ADNT, 2ADNT, DA6NT and unknown soluble products. TNT and its reduction products from chemical (iron and sulfide), aquatic plant, and Clostridia systems were not mineralized by either a

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49

Kunas, Kurt T. "Growth and injury of freely suspended animal cells in an agitated and surface-aerated bioreactor." Thesis, 1990. http://hdl.handle.net/1911/16360.

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The mechanism of animal cell damage due to agitation and aeration in a stirred bioreactor and the effect of media components on cell damage were investigated. Hybridoma cells ATCC CRL-8018 were grown in controlled bioreactors of 1-2 liter working volume, each agitated with a 7 cm-diameter impeller having pitched blades 1.8 cm wide by 7 cm tall. The experimental agitation speed was imposed at a consistent point in the exponential phase of batch growth in each culture. The apparent growth rate and cell yields on substrates and metabolites were used to assess the cell response. In bioreactor liqu

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50

Singh, Randeep. "Ester production and metabolic flux redistribution in Escherichia coli under aerobic conditions." Thesis, 2005. http://hdl.handle.net/1911/17918.

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Abstract:

Two alcohol acetyltransferases from Saccharomyces cerevisiae (ATF1 and ATF2) can catalyze the esterification of isoamyl alcohol and acetyl coenzyme A (acetyl-CoA). The respective genes were cloned from and expressed in an appropriate ack-pta strain of Escherichia coli. The new genetically engineered strains of E. coli produce isoamyl acetate, an ester, when isoamyl alcohol is added externally to the cell culture medium. Since acetyl-CoA is a substrate for this esterification reaction, the competing ackA-pta pathway at the acetyl-CoA node was inactivated to increase the intracellular acetyl-

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