Log in

Relevant bibliographies by topics / Chromatid cohesion / Journal articles

To see the other types of publications on this topic, follow the link: Chromatid cohesion.

Journal articles on the topic 'Chromatid cohesion'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Chromatid cohesion.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Mishra, Prashant K., Sultan Ciftci-Yilmaz, David Reynolds, et al. "Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis." Molecular Biology of the Cell 27, no. 14 (2016): 2286–300. http://dx.doi.org/10.1091/mbc.e16-01-0004.

Full text

Abstract:

Sister chromatid cohesion is essential for tension-sensing mechanisms that monitor bipolar attachment of replicated chromatids in metaphase. Cohesion is mediated by the association of cohesins along the length of sister chromatid arms. In contrast, centromeric cohesin generates intrastrand cohesion and sister centromeres, while highly cohesin enriched, are separated by >800 nm at metaphase in yeast. Removal of cohesin is necessary for sister chromatid separation during anaphase, and this is regulated by evolutionarily conserved polo-like kinase (Cdc5 in yeast, Plk1 in humans). Here we addre

APA, Harvard, Vancouver, ISO, and other styles

2

Sapkota, Hem, Emilia Wasiak, John R. Daum, and Gary J. Gorbsky. "Multiple determinants and consequences of cohesion fatigue in mammalian cells." Molecular Biology of the Cell 29, no. 15 (2018): 1811–24. http://dx.doi.org/10.1091/mbc.e18-05-0315.

Full text

Abstract:

Cells delayed in metaphase with intact mitotic spindles undergo cohesion fatigue, where sister chromatids separate asynchronously, while cells remain in mitosis. Cohesion fatigue requires release of sister chromatid cohesion. However, the pathways that breach sister chromatid cohesion during cohesion fatigue remain unknown. Using moderate-salt buffers to remove loosely bound chromatin cohesin, we show that “cohesive” cohesin is not released during chromatid separation during cohesion fatigue. Using a regulated protein heterodimerization system to lock different cohesin ring interfaces at speci

APA, Harvard, Vancouver, ISO, and other styles

3

Shi, Di, Shuaijun Zhao, Mei-Qing Zuo, et al. "The acetyltransferase Eco1 elicits cohesin dimerization during S phase." Journal of Biological Chemistry 295, no. 22 (2020): 7554–65. http://dx.doi.org/10.1074/jbc.ra120.013102.

Full text

Abstract:

Cohesin is a DNA-associated protein complex that forms a tripartite ring controlling sister chromatid cohesion, chromosome segregation and organization, DNA replication, and gene expression. Sister chromatid cohesion is established by the protein acetyltransferase Eco1, which acetylates two conserved lysine residues on the cohesin subunit Smc3 and thereby ensures correct chromatid separation in yeast (Saccharomyces cerevisiae) and other eukaryotes. However, the consequence of Eco1-catalyzed cohesin acetylation is unknown, and the exact nature of the cohesive state of chromatids remains controv

APA, Harvard, Vancouver, ISO, and other styles

4

Boavida, Ana, Diana Santos, Mohammad Mahtab, and Francesca M. Pisani. "Functional Coupling between DNA Replication and Sister Chromatid Cohesion Establishment." International Journal of Molecular Sciences 22, no. 6 (2021): 2810. http://dx.doi.org/10.3390/ijms22062810.

Full text

Abstract:

Several lines of evidence suggest the existence in the eukaryotic cells of a tight, yet largely unexplored, connection between DNA replication and sister chromatid cohesion. Tethering of newly duplicated chromatids is mediated by cohesin, an evolutionarily conserved hetero-tetrameric protein complex that has a ring-like structure and is believed to encircle DNA. Cohesin is loaded onto chromatin in telophase/G1 and converted into a cohesive state during the subsequent S phase, a process known as cohesion establishment. Many studies have revealed that down-regulation of a number of DNA replicati

APA, Harvard, Vancouver, ISO, and other styles

5

Chen, Yu-Fan, Chia-Ching Chou, and Marc R. Gartenberg. "Determinants of Sir2-Mediated, Silent Chromatin Cohesion." Molecular and Cellular Biology 36, no. 15 (2016): 2039–50. http://dx.doi.org/10.1128/mcb.00057-16.

Full text

Abstract:

Cohesin associates with distinct sites on chromosomes to mediate sister chromatid cohesion. Single cohesin complexes are thought to bind by encircling both sister chromatids in a topological embrace. Transcriptionally repressed chromosomal domains in the yeastSaccharomyces cerevisiaerepresent specialized sites of cohesion where cohesin binds silent chromatin in a Sir2-dependent fashion. In this study, we investigated the molecular basis for Sir2-mediated cohesion. We identified a cluster of charged surface residues of Sir2, collectively termed the EKDK motif, that are required for cohesin func

APA, Harvard, Vancouver, ISO, and other styles

6

Ochs, Fena, Charlotte Green, Aleksander Tomasz Szczurek, et al. "Sister chromatid cohesion is mediated by individual cohesin complexes." Science 383, no. 6687 (2024): 1122–30. http://dx.doi.org/10.1126/science.adl4606.

Full text

Abstract:

Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin’s stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclus

APA, Harvard, Vancouver, ISO, and other styles

7

Henrikus, Sarah S., and Alessandro Costa. "Towards a Structural Mechanism for Sister Chromatid Cohesion Establishment at the Eukaryotic Replication Fork." Biology 10, no. 6 (2021): 466. http://dx.doi.org/10.3390/biology10060466.

Full text

Abstract:

Cohesion between replicated chromosomes is essential for chromatin dynamics and equal segregation of duplicated genetic material. In the G1 phase, the ring-shaped cohesin complex is loaded onto duplex DNA, enriching at replication start sites, or “origins”. During the same phase of the cell cycle, and also at the origin sites, two MCM helicases are loaded as symmetric double hexamers around duplex DNA. During the S phase, and through the action of replication factors, cohesin switches from encircling one parental duplex DNA to topologically enclosing the two duplicated DNA filaments, which are

APA, Harvard, Vancouver, ISO, and other styles

8

Nakamura, Akito, Hiroyuki Arai, and Naoya Fujita. "Centrosomal Aki1 and cohesin function in separase-regulated centriole disengagement." Journal of Cell Biology 187, no. 5 (2009): 607–14. http://dx.doi.org/10.1083/jcb.200906019.

Full text

Abstract:

Sister chromatid separation at anaphase is triggered by cleavage of the cohesin subunit Scc1, which is mediated by separase. Centriole disengagement also requires separase. This dual role of separase permits concurrent control of these events for accurate metaphase to anaphase transition. Although the molecular mechanism underlying sister chromatid cohesion has been clarified, that of centriole cohesion is poorly understood. In this study, we show that Akt kinase–interacting protein 1 (Aki1) localizes to centrosomes and regulates centriole cohesion. Aki1 depletion causes formation of multipola

APA, Harvard, Vancouver, ISO, and other styles

9

Carvalhal, Sara, Alexandra Tavares, Mariana B. Santos, Mihailo Mirkovic, and Raquel A. Oliveira. "A quantitative analysis of cohesin decay in mitotic fidelity." Journal of Cell Biology 217, no. 10 (2018): 3343–53. http://dx.doi.org/10.1083/jcb.201801111.

Full text

Abstract:

Sister chromatid cohesion mediated by cohesin is essential for mitotic fidelity. It counteracts spindle forces to prevent premature chromatid individualization and random genome segregation. However, it is unclear what effects a partial decline of cohesin may have on chromosome organization. In this study, we provide a quantitative analysis of cohesin decay by inducing acute removal of defined amounts of cohesin from metaphase-arrested chromosomes. We demonstrate that sister chromatid cohesion is very resistant to cohesin loss as chromatid disjunction is only observed when chromosomes lose &am

APA, Harvard, Vancouver, ISO, and other styles

10

Stanyte, Rugile, Johannes Nuebler, Claudia Blaukopf, et al. "Dynamics of sister chromatid resolution during cell cycle progression." Journal of Cell Biology 217, no. 6 (2018): 1985–2004. http://dx.doi.org/10.1083/jcb.201801157.

Full text

Abstract:

Faithful genome transmission in dividing cells requires that the two copies of each chromosome’s DNA package into separate but physically linked sister chromatids. The linkage between sister chromatids is mediated by cohesin, yet where sister chromatids are linked and how they resolve during cell cycle progression has remained unclear. In this study, we investigated sister chromatid organization in live human cells using dCas9-mEGFP labeling of endogenous genomic loci. We detected substantial sister locus separation during G2 phase irrespective of the proximity to cohesin enrichment sites. Alm

APA, Harvard, Vancouver, ISO, and other styles

11

van Schie, Janne J. M., and Job de Lange. "The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks." Cells 10, no. 12 (2021): 3455. http://dx.doi.org/10.3390/cells10123455.

Full text

Abstract:

The cohesin complex facilitates faithful chromosome segregation by pairing the sister chromatids after DNA replication until mitosis. In addition, cohesin contributes to proficient and error-free DNA replication. Replisome progression and establishment of sister chromatid cohesion are intimately intertwined processes. Here, we review how the key factors in DNA replication and cohesion establishment cooperate in unperturbed conditions and during DNA replication stress. We discuss the detailed molecular mechanisms of cohesin recruitment and the entrapment of replicated sister chromatids at the r

APA, Harvard, Vancouver, ISO, and other styles

12

McNicoll, François, Anne Kühnel, Uddipta Biswas, et al. "Meiotic sex chromosome cohesion and autosomal synapsis are supported by Esco2." Life Science Alliance 3, no. 3 (2020): e201900564. http://dx.doi.org/10.26508/lsa.201900564.

Full text

Abstract:

In mitotic cells, establishment of sister chromatid cohesion requires acetylation of the cohesin subunit SMC3 (acSMC3) by ESCO1 and/or ESCO2. Meiotic cohesin plays additional but poorly understood roles in the formation of chromosome axial elements (AEs) and synaptonemal complexes. Here, we show that levels of ESCO2, acSMC3, and the pro-cohesion factor sororin increase on meiotic chromosomes as homologs synapse. These proteins are less abundant on the largely unsynapsed sex chromosomes, whose sister chromatid cohesion appears weaker throughout the meiotic prophase. Using three distinct conditi

APA, Harvard, Vancouver, ISO, and other styles

13

Jin, Hui, Vincent Guacci, and Hong-Guo Yu. "Pds5 is required for homologue pairing and inhibits synapsis of sister chromatids during yeast meiosis." Journal of Cell Biology 186, no. 5 (2009): 713–25. http://dx.doi.org/10.1083/jcb.200810107.

Full text

Abstract:

During meiosis, homologues become juxtaposed and synapsed along their entire length. Mutations in the cohesin complex disrupt not only sister chromatid cohesion but also homologue pairing and synaptonemal complex formation. In this study, we report that Pds5, a cohesin-associated protein known to regulate sister chromatid cohesion, is required for homologue pairing and synapsis in budding yeast. Pds5 colocalizes with cohesin along the length of meiotic chromosomes. In the absence of Pds5, the meiotic cohesin subunit Rec8 remains bound to chromosomes with only minor defects in sister chromatid

APA, Harvard, Vancouver, ISO, and other styles

14

Rollins, Robert A., Maria Korom, Nathalie Aulner, Andrew Martens, and Dale Dorsett. "Drosophila Nipped-B Protein Supports Sister Chromatid Cohesion and Opposes the Stromalin/Scc3 Cohesion Factor To Facilitate Long-Range Activation of the cut Gene." Molecular and Cellular Biology 24, no. 8 (2004): 3100–3111. http://dx.doi.org/10.1128/mcb.24.8.3100-3111.2004.

Full text

Abstract:

ABSTRACT The Drosophila melanogaster Nipped-B protein facilitates transcriptional activation of the cut and Ultrabithorax genes by remote enhancers. Sequence homologues of Nipped-B, Scc2 of Saccharomyces cerevisiae, and Mis4 of Schizosaccharomyces pombe are required for sister chromatid cohesion during mitosis. The evolutionarily conserved Cohesin protein complex mediates sister chromatid cohesion, and Scc2 and Mis4 are needed for Cohesin to associate with chromosomes. Here, we show that Nipped-B is also required for sister chromatid cohesion but that, opposite to the effect of Nipped-B, the s

APA, Harvard, Vancouver, ISO, and other styles

15

Warren, Cheryl D., D. Mark Eckley, Marina S. Lee, et al. "S-Phase Checkpoint Genes Safeguard High-Fidelity Sister Chromatid Cohesion." Molecular Biology of the Cell 15, no. 4 (2004): 1724–35. http://dx.doi.org/10.1091/mbc.e03-09-0637.

Full text

Abstract:

Cohesion establishment and maintenance are carried out by proteins that modify the activity of Cohesin, an essential complex that holds sister chromatids together. Constituents of the replication fork, such as the DNA polymerase α-binding protein Ctf4, contribute to cohesion in ways that are poorly understood. To identify additional cohesion components, we analyzed a ctf4Δ synthetic lethal screen performed on microarrays. We focused on a subset of ctf4Δ-interacting genes with genetic instability of their own. Our analyses revealed that 17 previously studied genes are also necessary for the mai

APA, Harvard, Vancouver, ISO, and other styles

16

Brar, Gloria A., Andreas Hochwagen, Ly-sha S. Ee, and Angelika Amon. "The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing." Molecular Biology of the Cell 20, no. 3 (2009): 1030–47. http://dx.doi.org/10.1091/mbc.e08-06-0637.

Full text

Abstract:

Sister chromatid cohesion, mediated by cohesin complexes, is laid down during DNA replication and is essential for the accurate segregation of chromosomes. Previous studies indicated that, in addition to their cohesion function, cohesins are essential for completion of recombination, pairing, meiotic chromosome axis formation, and assembly of the synaptonemal complex (SC). Using mutants in the cohesin subunit Rec8, in which phosphorylated residues were mutated to alanines, we show that cohesin phosphorylation is not only important for cohesin removal, but that cohesin's meiotic prophase functi

APA, Harvard, Vancouver, ISO, and other styles

17

Eng, Thomas, Vincent Guacci, and Douglas Koshland. "Interallelic complementation provides functional evidence for cohesin–cohesin interactions on DNA." Molecular Biology of the Cell 26, no. 23 (2015): 4224–35. http://dx.doi.org/10.1091/mbc.e15-06-0331.

Full text

Abstract:

The cohesin complex (Mcd1p, Smc1p, Smc3p, and Scc3p) has multiple roles in chromosome architecture, such as promoting sister chromatid cohesion, chromosome condensation, DNA repair, and transcriptional regulation. The prevailing embrace model for sister chromatid cohesion posits that a single cohesin complex entraps both sister chromatids. We report interallelic complementation between pairs of nonfunctional mcd1 alleles (mcd1-1 and mcd1-Q266) or smc3 alleles (smc3-42 and smc3-K113R). Cells bearing individual mcd1 or smc3 mutant alleles are inviable and defective for both sister chromatid cohe

APA, Harvard, Vancouver, ISO, and other styles

18

Ghaddar, Nagham, Pierre Luciano, Vincent Géli, and Yves Corda. "Chromatin assembly factor-1 preserves genome stability in ctf4∆ cells by promoting sister chromatid cohesion." Cell Stress 7, no. 9 (2023): 69–89. http://dx.doi.org/10.15698/cst2023.09.289.

Full text

Abstract:

Chromatin assembly and the establishment of sister chromatid cohesion are intimately connected to the progression of DNA replication forks. Here we examined the genetic interaction between the heterotrimeric chromatin assembly factor-1 (CAF-1), a central component of chromatin assembly during replication, and the core replisome component Ctf4. We find that CAF-1 deficient cells as well as cells affected in newly-synthesized H3-H4 histones deposition during DNA replication exhibit a severe negative growth with ctf4∆ mutant. We dissected the role of CAF-1 in the maintenance of genome stability i

APA, Harvard, Vancouver, ISO, and other styles

19

Suja, J. A., C. Antonio, A. Debec, and J. S. Rufas. "Phosphorylated proteins are involved in sister-chromatid arm cohesion during meiosis I." Journal of Cell Science 112, no. 17 (1999): 2957–69. http://dx.doi.org/10.1242/jcs.112.17.2957.

Full text

Abstract:

Sister-chromatid arm cohesion is lost during the metaphase I/anaphase I transition to allow homologue separation. To obtain needed information on this process we have analysed in grasshopper bivalents the sequential release of arm cohesion in relation to the behaviour of chromatid axes. Results show that sister axes are associated during early metaphase I but separate during late metaphase I leading to a concomitant change of chromosome structure that implies the loss of sister-kinetochore cohesion. Afterwards, homologues initiate their separation asynchronously depending on their size, and nu

APA, Harvard, Vancouver, ISO, and other styles

20

Chiroli, Elena, Valentina Rossio, Giovanna Lucchini, and Simonetta Piatti. "The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects." Journal of Cell Biology 177, no. 4 (2007): 599–611. http://dx.doi.org/10.1083/jcb.200609088.

Full text

Abstract:

Faithful chromosome transmission requires establishment of sister chromatid cohesion during S phase, followed by its removal at anaphase onset. Sister chromatids are tethered together by cohesin, which is displaced from chromosomes through cleavage of its Mcd1 subunit by the separase protease. Separase is in turn inhibited, up to this moment, by securin. Budding yeast cells respond to morphogenetic defects by a transient arrest in G2 with high securin levels and unseparated chromatids. We show that neither securin elimination nor forced cohesin cleavage is sufficient for anaphase in these cond

APA, Harvard, Vancouver, ISO, and other styles

21

Lee, Janice Y., Aki Hayashi-Hagihara, and Terry L. Orr-Weaver. "Roles and regulation of the Drosophila centromere cohesion protein MEI-S332 family." Philosophical Transactions of the Royal Society B: Biological Sciences 360, no. 1455 (2005): 543–52. http://dx.doi.org/10.1098/rstb.2005.1619.

Full text

Abstract:

In meiosis, a physical attachment, or cohesion, between the centromeres of the sister chromatids is retained until their separation at anaphase II. This cohesion is essential for ensuring accurate segregation of the sister chromatids in meiosis II and avoiding aneuploidy, a condition that can lead to prenatal lethality or birth defects. The Drosophila MEI-S332 protein localizes to centromeres when sister chromatids are attached in mitosis and meiosis, and it is required to maintain cohesion at the centromeres after cohesion along the sister chromatid arms is lost at the metaphase I/anaphase I

APA, Harvard, Vancouver, ISO, and other styles

22

Uchiyama, Susumu, and Kiichi Fukui. "Condensin in Chromatid Cohesion and Segregation." Cytogenetic and Genome Research 147, no. 4 (2015): 212–16. http://dx.doi.org/10.1159/000444868.

Full text

Abstract:

After replication of genomic DNA during the S phase, 2 chromatids hold together longitudinally. When cells enter mitosis, the paired sister chromatids start to condense and then segregate into individual chromatids except for the centromeric region. Upon attachment of microtubules to the kinetochore, subsequent pulling of the 2 sister chromatids by the spindles towards opposite poles results in 2 completely separated chromatids. Besides more than 100 kinds of kinetochore proteins, several key proteins such as cohesin, separase, shugoshin, and condensin contribute to chromatid cohesion and segr

APA, Harvard, Vancouver, ISO, and other styles

23

Santos, Diana, Mohammad Mahtab, Ana Boavida, and Francesca M. Pisani. "Role of the DDX11 DNA Helicase in Warsaw Breakage Syndrome Etiology." International Journal of Molecular Sciences 22, no. 5 (2021): 2308. http://dx.doi.org/10.3390/ijms22052308.

Full text

Abstract:

Warsaw breakage syndrome (WABS) is a genetic disorder characterized by sister chromatid cohesion defects, growth retardation, microcephaly, hearing loss and other variable clinical manifestations. WABS is due to biallelic mutations of the gene coding for the super-family 2 DNA helicase DDX11/ChlR1, orthologous to the yeast chromosome loss protein 1 (Chl1). WABS is classified in the group of “cohesinopathies”, rare hereditary diseases that are caused by mutations in genes coding for subunits of the cohesin complex or protein factors having regulatory roles in the sister chromatid cohesion proce

APA, Harvard, Vancouver, ISO, and other styles

24

Yan, Rihui, Sharon E. Thomas, Jui-He Tsai, Yukihiro Yamada, and Bruce D. McKee. "SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster." Journal of Cell Biology 188, no. 3 (2010): 335–49. http://dx.doi.org/10.1083/jcb.200904040.

Full text

Abstract:

Sister chromatid cohesion is essential to maintain stable connections between homologues and sister chromatids during meiosis and to establish correct centromere orientation patterns on the meiosis I and II spindles. However, the meiotic cohesion apparatus in Drosophila melanogaster remains largely uncharacterized. We describe a novel protein, sisters on the loose (SOLO), which is essential for meiotic cohesion in Drosophila. In solo mutants, sister centromeres separate before prometaphase I, disrupting meiosis I centromere orientation and causing nondisjunction of both homologous and sister c

APA, Harvard, Vancouver, ISO, and other styles

25

Muñoz, Sofía, Francesca Passarelli, and Frank Uhlmann. "Conserved roles of chromatin remodellers in cohesin loading onto chromatin." Current Genetics 66, no. 5 (2020): 951–56. http://dx.doi.org/10.1007/s00294-020-01075-x.

Full text

Abstract:

Abstract Cohesin is a conserved, ring-shaped protein complex that topologically entraps DNA. This ability makes this member of the structural maintenance of chromosomes (SMC) complex family a central hub of chromosome dynamics regulation. Besides its essential role in sister chromatid cohesion, cohesin shapes the interphase chromatin domain architecture and plays important roles in transcriptional regulation and DNA repair. Cohesin is loaded onto chromosomes at centromeres, at the promoters of highly expressed genes, as well as at DNA replication forks and sites of DNA damage. However, the fea

APA, Harvard, Vancouver, ISO, and other styles

26

Mehta, Gunjan D., Ravinder Kumar, Sanjeeva Srivastava, and Santanu Kumar Ghosh. "Cohesin: Functions beyond sister chromatid cohesion." FEBS Letters 587, no. 15 (2013): 2299–312. http://dx.doi.org/10.1016/j.febslet.2013.06.035.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Morrison, C., P. Vagnarelli, E. Sonoda, S. Takeda, and W. C. Earnshaw. "Sister chromatid cohesion and genome stability in vertebrate cells." Biochemical Society Transactions 31, no. 1 (2003): 263–65. http://dx.doi.org/10.1042/bst0310263.

Full text

Abstract:

For successful eukaryotic mitosis, sister chromatid pairs remain linked after replication until their kinetochores have been attached to opposite spindle poles by microtubules. This linkage is broken at the metaphase–anaphase transition and the sisters separate. In budding yeast, this sister chromatid cohesion requires a multi-protein complex called cohesin. A key component of cohesin is Scc1/Mcd1 (Rad21 in fission yeast). Disruption of the chicken orthologue of Scc1 by gene targeting in DT40 cells causes premature sister chromatid separation. Cohesion between sister chromatids is likely to pr

APA, Harvard, Vancouver, ISO, and other styles

28

Nasmyth, Kim, and Alexander Schleiffer. "From a single double helix to paired double helices and back." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 359, no. 1441 (2004): 99–108. http://dx.doi.org/10.1098/rstb.2003.1417.

Full text

Abstract:

The propagation of our genomes during cell proliferation depends on the movement of sister DNA molecules produced by DNA replication to opposite sides of the cell before it divides. This feat is achieved by microtubules in eukaryotic cells but it has long remained a mystery how cells ensure that sister DNAs attach to microtubules with opposite orientations, known as amphitelic attachment. It is currently thought that sister chromatid cohesion has a crucial role. By resisting the forces exerted by microtubules, sister chromatid cohesion gives rise to tension that is thought essential for stabil

APA, Harvard, Vancouver, ISO, and other styles

29

Kateneva, Anna V., Anton A. Konovchenko, Vincent Guacci, and Michael E. Dresser. "Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae." Journal of Cell Biology 171, no. 2 (2005): 241–53. http://dx.doi.org/10.1083/jcb.200505020.

Full text

Abstract:

Sister chromatid cohesion and interhomologue recombination are coordinated to promote the segregation of homologous chromosomes instead of sister chromatids at the first meiotic division. During meiotic prophase in Saccharomyces cerevisiae, the meiosis-specific cohesin Rec8p localizes along chromosome axes and mediates most of the cohesion. The mitotic cohesin Mcd1p/Scc1p localizes to discrete spots along chromosome arms, and its function is not clear. In cells lacking Tid1p, which is a member of the SWI2/SNF2 family of helicase-like proteins that are involved in chromatin remodeling, Mcd1p an

APA, Harvard, Vancouver, ISO, and other styles

30

Hou, Fajian, Chih-Wen Chu, Xiangduo Kong, Kyoko Yokomori, and Hui Zou. "The acetyltransferase activity of San stabilizes the mitotic cohesin at the centromeres in a shugoshin-independent manner." Journal of Cell Biology 177, no. 4 (2007): 587–97. http://dx.doi.org/10.1083/jcb.200701043.

Full text

Abstract:

Proper sister chromatid cohesion is critical for maintaining genetic stability. San is a putative acetyltransferase that is important for sister chromatid cohesion in Drosophila melanogaster, but not in budding yeast. We showed that San is critical for sister chromatid cohesion in HeLa cells, suggesting that this mechanism may be conserved in metazoans. Furthermore, although a small fraction of San interacts with the NatA complex, San appears to mediate cohesion independently. San exhibits acetyltransferase activity in vitro, and its activity is required for sister chromatid cohesion in vivo.

APA, Harvard, Vancouver, ISO, and other styles

31

Revenkova, E., and R. Jessberger. "Keeping sister chromatids together: cohesins in meiosis." Reproduction 130, no. 6 (2005): 783–90. http://dx.doi.org/10.1530/rep.1.00864.

Full text

Abstract:

Meiosis poses unique challenges to chromosome dynamics. Before entry into meiosis, each chromosome is duplicated and gives rise to two sister chromatids linked to each other by cohesion. Production of haploid gametes requires segregation of homologous chromosomes in the first meiotic division and of sister chromatids in the second. To ensure precise distribution of chromosomes to the daughter cells, sister chromatid cohesion (SCC) has to be dissolved in two steps. Maintenance and regulation of SCC is performed by the cohesin protein complex. This short review will primarily focus on the core c

APA, Harvard, Vancouver, ISO, and other styles

32

Lin, Weiqiang, Hui Jin, Xiuwen Liu, Kristin Hampton, and Hong-Guo Yu. "Scc2 regulates gene expression by recruiting cohesin to the chromosome as a transcriptional activator during yeast meiosis." Molecular Biology of the Cell 22, no. 12 (2011): 1985–96. http://dx.doi.org/10.1091/mbc.e10-06-0545.

Full text

Abstract:

To tether sister chromatids, a protein-loading complex, including Scc2, recruits cohesin to the chromosome at discrete loci. Cohesin facilitates the formation of a higher-order chromosome structure that could also influence gene expression. How cohesin directly regulates transcription remains to be further elucidated. We report that in budding yeast Scc2 is required for sister-chromatid cohesion during meiosis for two reasons. First, Scc2 is required for activating the expression of REC8, which encodes a meiosis-specific cohesin subunit; second, Scc2 is necessary for recruiting meiotic cohesin

APA, Harvard, Vancouver, ISO, and other styles

33

McNally, Karen P., Elizabeth A. Beath, Brennan M. Danlasky, et al. "Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans." PLOS Genetics 18, no. 10 (2022): e1010136. http://dx.doi.org/10.1371/journal.pgen.1010136.

Full text

Abstract:

Accurate chromosome segregation requires a cohesin-mediated physical attachment between chromosomes that are to be segregated apart, and a bipolar spindle with microtubule plus ends emanating from exactly two poles toward the paired chromosomes. We asked whether the striking bipolar structure of C. elegans meiotic chromosomes is required for bipolarity of acentriolar female meiotic spindles by time-lapse imaging of mutants that lack cohesion between chromosomes. Both a spo-11 rec-8 coh-4 coh-3 quadruple mutant and a spo-11 rec-8 double mutant entered M phase with separated sister chromatids la

APA, Harvard, Vancouver, ISO, and other styles

34

Oliveira, Raquel A., and Kim Nasmyth. "Getting through anaphase: splitting the sisters and beyond." Biochemical Society Transactions 38, no. 6 (2010): 1639–44. http://dx.doi.org/10.1042/bst0381639.

Full text

Abstract:

Sister-chromatid cohesion, thought to be primarily mediated by the cohesin complex, is essential for chromosome segregation. The forces holding the two sisters resist the tendency of microtubules to prematurely pull sister DNAs apart and thereby prevent random segregation of the genome during mitosis, and consequent aneuploidy. By counteracting the spindle pulling forces, cohesion between the two sisters generates the tension necessary to stabilize microtubule–kinetochore attachments. Upon entry into anaphase, however, the linkages that hold the two sister DNAs must be rapidly destroyed to all

APA, Harvard, Vancouver, ISO, and other styles

35

Peters, J. M., and T. Nishiyama. "Sister Chromatid Cohesion." Cold Spring Harbor Perspectives in Biology 4, no. 11 (2012): a011130. http://dx.doi.org/10.1101/cshperspect.a011130.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Rieder, C. L., and R. Cole. "Chromatid cohesion during mitosis: lessons from meiosis." Journal of Cell Science 112, no. 16 (1999): 2607–13. http://dx.doi.org/10.1242/jcs.112.16.2607.

Full text

Abstract:

The equal distribution of chromosomes during mitosis and meiosis is dependent on the maintenance of sister chromatid cohesion. In this commentary we review the evidence that, during meiosis, the mechanism underlying the cohesion of chromatids along their arms is different from that responsible for cohesion in the centromere region. We then argue that the chromatids on a mitotic chromosome are also tethered along their arms and in the centromere by different mechanisms, and that the functional action of these two mechanisms can be temporally separated under various conditions. Finally, we demon

APA, Harvard, Vancouver, ISO, and other styles

37

Alomer, Reem M., Eulália M. L. da Silva, Jingrong Chen, et al. "Esco1 and Esco2 regulate distinct cohesin functions during cell cycle progression." Proceedings of the National Academy of Sciences 114, no. 37 (2017): 9906–11. http://dx.doi.org/10.1073/pnas.1708291114.

Full text

Abstract:

Sister chromatids are tethered together by the cohesin complex from the time they are made until their separation at anaphase. The ability of cohesin to tether sister chromatids together depends on acetylation of its Smc3 subunit by members of the Eco1 family of cohesin acetyltransferases. Vertebrates express two orthologs of Eco1, called Esco1 and Esco2, both of which are capable of modifying Smc3, but their relative contributions to sister chromatid cohesion are unknown. We therefore set out to determine the precise contributions of Esco1 and Esco2 to cohesion in vertebrate cells. Here we sh

APA, Harvard, Vancouver, ISO, and other styles

38

Bender, Dawn, Eulália Maria Lima Da Silva, Jingrong Chen, et al. "Multivalent interaction of ESCO2 with the replication machinery is required for sister chromatid cohesion in vertebrates." Proceedings of the National Academy of Sciences 117, no. 2 (2019): 1081–89. http://dx.doi.org/10.1073/pnas.1911936117.

Full text

Abstract:

The tethering together of sister chromatids by the cohesin complex ensures their accurate alignment and segregation during cell division. In vertebrates, sister chromatid cohesion requires the activity of the ESCO2 acetyltransferase, which modifies the Smc3 subunit of cohesin. It was shown recently that ESCO2 promotes cohesion through interaction with the MCM replicative helicase. However, ESCO2 does not significantly colocalize with the MCM complex, suggesting there are additional interactions important for ESCO2 function. Here we show that ESCO2 is recruited to replication factories, sites o

APA, Harvard, Vancouver, ISO, and other styles

39

Losada, Ana, Tomoki Yokochi, Ryuji Kobayashi, and Tatsuya Hirano. "Identification and Characterization of Sa/Scc3p Subunits in the Xenopus and Human Cohesin Complexes." Journal of Cell Biology 150, no. 3 (2000): 405–16. http://dx.doi.org/10.1083/jcb.150.3.405.

Full text

Abstract:

A multisubunit protein complex, termed cohesin, plays an essential role in sister chromatid cohesion in yeast and in Xenopus laevis cell-free extracts. We report here that two distinct cohesin complexes exist in Xenopus egg extracts. A 14S complex (x-cohesinSA1) contains XSMC1, XSMC3, XRAD21, and a newly identified subunit, XSA1. In a second 12.5S complex (x-cohesinSA2), XSMC1, XSMC3, and XRAD21 associate with a different subunit, XSA2. Both XSA1 and XSA2 belong to the SA family of mammalian proteins and exhibit similarity to Scc3p, a recently identified component of yeast cohesin. In Xenopus

APA, Harvard, Vancouver, ISO, and other styles

40

Stephens, Andrew D., Julian Haase, Leandra Vicci, Russell M. Taylor, and Kerry Bloom. "Cohesin, condensin, and the intramolecular centromere loop together generate the mitotic chromatin spring." Journal of Cell Biology 193, no. 7 (2011): 1167–80. http://dx.doi.org/10.1083/jcb.201103138.

Full text

Abstract:

Sister chromatid cohesion provides the mechanistic basis, together with spindle microtubules, for generating tension between bioriented chromosomes in metaphase. Pericentric chromatin forms an intramolecular loop that protrudes bidirectionally from the sister chromatid axis. The centromere lies on the surface of the chromosome at the apex of each loop. The cohesin and condensin structural maintenance of chromosomes (SMC) protein complexes are concentrated within the pericentric chromatin, but whether they contribute to tension-generating mechanisms is not known. To understand how pericentric c

APA, Harvard, Vancouver, ISO, and other styles

41

Finardi, Alice, Lucia F. Massari, and Rosella Visintin. "Anaphase Bridges: Not All Natural Fibers Are Healthy." Genes 11, no. 8 (2020): 902. http://dx.doi.org/10.3390/genes11080902.

Full text

Abstract:

At each round of cell division, the DNA must be correctly duplicated and distributed between the two daughter cells to maintain genome identity. In order to achieve proper chromosome replication and segregation, sister chromatids must be recognized as such and kept together until their separation. This process of cohesion is mainly achieved through proteinaceous linkages of cohesin complexes, which are loaded on the sister chromatids as they are generated during S phase. Cohesion between sister chromatids must be fully removed at anaphase to allow chromosome segregation. Other (non-proteinaceo

APA, Harvard, Vancouver, ISO, and other styles

42

Edwards, Shaune, Caroline M. Li, Daniel L. Levy, Jessica Brown, Peter M. Snow та Judith L. Campbell. "Saccharomyces cerevisiae DNA Polymerase ε and Polymerase σ Interact Physically and Functionally, Suggesting a Role for Polymerase ε in Sister Chromatid Cohesion". Molecular and Cellular Biology 23, № 8 (2003): 2733–48. http://dx.doi.org/10.1128/mcb.23.8.2733-2748.2003.

Full text

Abstract:

ABSTRACT The large subunit of Saccharomyces cerevisiae DNA polymerase ε, Pol2, comprises two essential functions. The N terminus has essential DNA polymerase activity. The C terminus is also essential, but its function is unknown. We report here that the C-terminal domain of Pol2 interacts with polymerase σ (Pol σ), a recently identified, essential nuclear nucleotidyl transferase encoded by two redundant genes, TRF4 and TRF5. This interaction is functional, since Pol σ stimulates the polymerase activity of the Pol ε holoenzyme significantly. Since Trf4 is required for sister chromatid cohesion

APA, Harvard, Vancouver, ISO, and other styles

43

Kong, Xiangduo, Alexander R. Ball, Eiichiro Sonoda, et al. "Cohesin Associates with Spindle Poles in a Mitosis-specific Manner and Functions in Spindle Assembly in Vertebrate Cells." Molecular Biology of the Cell 20, no. 5 (2009): 1289–301. http://dx.doi.org/10.1091/mbc.e08-04-0419.

Full text

Abstract:

Cohesin is an essential protein complex required for sister chromatid cohesion. Cohesin associates with chromosomes and establishes sister chromatid cohesion during interphase. During metaphase, a small amount of cohesin remains at the chromosome-pairing domain, mainly at the centromeres, whereas the majority of cohesin resides in the cytoplasm, where its functions remain unclear. We describe the mitosis-specific recruitment of cohesin to the spindle poles through its association with centrosomes and interaction with nuclear mitotic apparatus protein (NuMA). Overexpression of NuMA enhances coh

APA, Harvard, Vancouver, ISO, and other styles

44

Borrie, Melinda S., John S. Campor, Hansa Joshi, and Marc R. Gartenberg. "Binding, sliding, and function of cohesin during transcriptional activation." Proceedings of the National Academy of Sciences 114, no. 7 (2017): E1062—E1071. http://dx.doi.org/10.1073/pnas.1617309114.

Full text

Abstract:

The ring-shaped cohesin complex orchestrates long-range DNA interactions to mediate sister chromatid cohesion and other aspects of chromosome structure and function. In the yeast Saccharomyces cerevisiae, the complex binds discrete sites along chromosomes, including positions within and around genes. Transcriptional activity redistributes the complex to the 3′ ends of convergently oriented gene pairs. Despite the wealth of information about where cohesin binds, little is known about cohesion at individual chromosomal binding sites and how transcription affects cohesion when cohesin complexes r

APA, Harvard, Vancouver, ISO, and other styles

45

Wang, Shao-Win, Rebecca L. Read, and Chris J. Norbury. "Fission yeast Pds5 is required for accurate chromosome segregation and for survival after DNA damage or metaphase arrest." Journal of Cell Science 115, no. 3 (2002): 587–98. http://dx.doi.org/10.1242/jcs.115.3.587.

Full text

Abstract:

Sister chromatid cohesion, which is established during the S phase of the eukaryotic cell cycle and persists until the onset of anaphase, is essential for the maintenance of genomic integrity. Cohesion requires the multi-protein complex cohesin, as well as a number of accessory proteins including Pds5/BIMD/Spo76. In the budding yeast Saccharomyces cerevisiae Pds5 is an essential protein that localises to chromosomes in a cohesin-dependent manner. Here we describe the characterisation in the fission yeast Schizosaccharomyces pombe of pds5+, a novel,non-essential orthologue of S. cerevisiae PDS5

APA, Harvard, Vancouver, ISO, and other styles

46

Gregson, Heather C., John A. Schmiesing, Jong-Soo Kim, Toshiki Kobayashi, Sharleen Zhou, and Kyoko Yokomori. "A Potential Role for Human Cohesin in Mitotic Spindle Aster Assembly." Journal of Biological Chemistry 276, no. 50 (2001): 47575–82. http://dx.doi.org/10.1074/jbc.m103364200.

Full text

Abstract:

The cohesin multiprotein complex containing SMC1, SMC3, Scc3 (SA), and Scc1 (Rad21) is required for sister chromatid cohesion in eukaryotes. Although metazoan cohesin associates with chromosomes and was shown to function in the establishment of sister chromatid cohesion during interphase, the majority of cohesin was found to be off chromosomes and reside in the cytoplasm in metaphase. Despite its dissociation from chromosomes, however, microinjection of an antibody against human SMC1 led to disorganization of the metaphase plate and cell cycle arrest, indicating that human cohesin still plays

APA, Harvard, Vancouver, ISO, and other styles

47

Short, Ben. "The different ties that bind." Journal of Cell Biology 187, no. 2 (2009): 151. http://dx.doi.org/10.1083/jcb.1872if.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Rahman, Sadia, Mathew J. K. Jones, and Prasad V. Jallepalli. "Cohesin recruits the Esco1 acetyltransferase genome wide to repress transcription and promote cohesion in somatic cells." Proceedings of the National Academy of Sciences 112, no. 36 (2015): 11270–75. http://dx.doi.org/10.1073/pnas.1505323112.

Full text

Abstract:

The cohesin complex links DNA molecules and plays key roles in the organization, expression, repair, and segregation of eukaryotic genomes. In vertebrates the Esco1 and Esco2 acetyltransferases both modify cohesin’s Smc3 subunit to establish sister chromatid cohesion during S phase, but differ in their N-terminal domains and expression during development and across the cell cycle. Here we show that Esco1 and Esco2 also differ dramatically in their interaction with chromatin, as Esco1 is recruited by cohesin to over 11,000 sites, whereas Esco2 is infrequently enriched at REST/NRSF target genes.

APA, Harvard, Vancouver, ISO, and other styles

49

Eijpe, Maureen, Hildo Offenberg, Rolf Jessberger, Ekaterina Revenkova та Christa Heyting. "Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1β and SMC3". Journal of Cell Biology 160, № 5 (2003): 657–70. http://dx.doi.org/10.1083/jcb.200212080.

Full text

Abstract:

In meiotic prophase, the sister chromatids of each chromosome develop a common axial element (AE) that is integrated into the synaptonemal complex (SC). We analyzed the incorporation of sister chromatid cohesion proteins (cohesins) and other AE components into AEs. Meiotic cohesin REC8 appeared shortly before premeiotic S phase in the nucleus and formed AE-like structures (REC8-AEs) from premeiotic S phase on. Subsequently, meiotic cohesin SMC1β, cohesin SMC3, and AE proteins SCP2 and SCP3 formed dots along REC8-AEs, which extended and fused until they lined REC8-AEs along their length. In met

APA, Harvard, Vancouver, ISO, and other styles

50

Watanabe, Yoshinori, and Tomoya S. Kitajima. "Shugoshin protects cohesin complexes at centromeres." Philosophical Transactions of the Royal Society B: Biological Sciences 360, no. 1455 (2005): 515–21. http://dx.doi.org/10.1098/rstb.2004.1607.

Full text

Abstract:

The different regulation of sister chromatid cohesion at centromeres and along chromosome arms is obvious during meiosis, because centromeric cohesion, but not arm cohesion, persists throughout anaphase of the first division. A protein required to protect centromeric cohesin Rec8 from separase cleavage has been identified and named shugoshin (or Sgo1) after shugoshin (‘guardian spirit’ in Japanese). It has become apparent that shugoshin shows marginal homology with Drosophila Mei-S332 and several uncharacterized proteins in other eukaryotic organisms. Because Mei-S332 is a protein previously s

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!