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Relevant bibliographies by topics / Culture affinity

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Academic literature on the topic 'Culture affinity'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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Journal articles on the topic "Culture affinity"

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Garle, Michael J., and Jeffrey R. Fry. "A Comparison of Hepatic Enzyme Activities and their Modulation by Dexamethazone in Freshly Isolated and Cultured Hepatocytes and in the Differentiated Hepatoma Cell Line, 2sFou." Alternatives to Laboratory Animals 24, no. 1 (1996): 31–37. http://dx.doi.org/10.1177/026119299602400106.

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Rodent hepatocytes are mitotically inhibited and lose hepatospecific functions over time in culture. In contrast, some differentiated hepatoma cell lines express stable hepatospecific functions in culture, but at much lower levels than those initially found in primary hepatocytes. A number of hepatospecific functions were measured in freshly isolated and cultured rat hepatocytes; these were compared to activities found in the differentiated Reuber hepatoma cell line, 2sFou. The effects of dexamethazone on these activities were also investigated, since dexamethazone is reported to enhance the e

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Smith, John. "The trinitarian dance with culture: Trinity as the missiological optic for understanding culture." Missiology: An International Review 48, no. 2 (2020): 154–68. http://dx.doi.org/10.1177/0091829619887386.

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In discussions of imago Dei, we largely confine ourselves to functional and moral affinity to God, that is that we have been made in God’s image with the result that obedience to God amounts to imitating his moral character and behavior. However, we need to add the existential affinity of God’s being to our understanding of God’s image in us, specifically the fact that he is both singular (in essence) and plural (in person). To some degree, people share God’s singular–plural quality and that one–many affinity can give great insight into gospel ministry. Moreover, as with the moral dimension of

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Roos, Joseph W., and Martin A. Hjortso. "Control of anEscherichia colimixed culture via affinity binding." Biotechnology and Bioengineering 38, no. 4 (1991): 380–88. http://dx.doi.org/10.1002/bit.260380408.

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Suranovic, Steven, and Robert Winthrop. "Trade Liberalization and Culture." Global Economy Journal 14, no. 1 (2014): 57–78. http://dx.doi.org/10.1515/gej-2013-0047.

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This paper addresses the effect of international trade on cultural outcomes from both economic and anthropological perspectives. Definitions of culture are informed by anthropology and then incorporated into a standard economic trade models in two distinct ways. In the “cultural affinity from work” model, workers receive a non-pecuniary cultural benefit from work in a particular industry. In the “cultural externality” model, consumers of a product receive utility from other consumer’s consumption of a domestic good. We show that resistance to change due to cultural concerns can reduce the nati

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Dickman, K. G., and J. L. Renfro. "Primary culture of flounder renal tubule cells: transepithelial transport." American Journal of Physiology-Renal Physiology 251, no. 3 (1986): F424—F432. http://dx.doi.org/10.1152/ajprenal.1986.251.3.f424.

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Renal proximal tubule cells from the winter flounder (Pseudopleuronectes americanus) were maintained in a functionally differentiated state for up to 16 days in primary culture on floating collagen gels. The cells were confluent after 7-8 days in culture, contracted the collagen gels, and exhibited ciliary activity. Electron microscopy indicated that the cultures were composed of continuous sheets of columnar epithelial cells that had established structural polarity. When mounted in Ussing chambers, the cultures exhibited a small mucosa-negative potential difference (0.6 +/- 0.10 mV) and a low

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van Dijk, H. P., M. J. Kroos, J. S. Starreveld, et al. "Expression of haemopexin receptors by cultured human cytotrophoblast." Biochemical Journal 307, no. 3 (1995): 669–72. http://dx.doi.org/10.1042/bj3070669.

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The expression of cell-surface haemopexin (Hx) receptors on human cytotrophoblasts was assessed by using four different Hx species purified from plasma: human Hx isolated by wheatgerm-affinity chromatography, human Hx isolated by haem-agarose-affinity chromatography and rabbit and rat Hx, also isolated by haem-agarose-affinity chromatography. About 3500-7000 high-affinity (Kd 0.34-0.85 nM) receptors per cell were measured by Scatchard-type analysis at 4 degrees C using human (species obtained by both methods) or rabbit 125I-labelled haem-Hx. Measured simultaneously, transferrin receptor number

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Eckel, J., G. van Echten, and H. Reinauer. "Adult cardiac myocytes in primary culture: cell characteristics and insulin-receptor interaction." American Journal of Physiology-Heart and Circulatory Physiology 249, no. 2 (1985): H212—H221. http://dx.doi.org/10.1152/ajpheart.1985.249.2.h212.

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Calcium-tolerant adult cardiac myocytes were kept in culture under serum-free conditions in the presence of physiological concentrations of insulin. Up to 4 days, 70% of cells retained their in vivo rodshaped morphology without gross structural alterations. During that period a constant ATP-to-ADP ratio was observed with a mean value of 10.6 +/- 0.5 (n = 4). The rate of [14C]phenylalanine incorporation remained unaltered up to 63 h in culture. Insulin binding to cultured cells was found to be time-and temperature-dependent, reversible, and highly specific. Scatchard analysis of equilibrium bin

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Sinclair, P. R., W. J. Bement, N. Gorman, H. H. Liem, A. W. Wolkoff, and U. Muller-Eberhard. "Effect of serum proteins on haem uptake and metabolism in primary cultures of liver cells." Biochemical Journal 256, no. 1 (1988): 159–65. http://dx.doi.org/10.1042/bj2560159.

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A role of haemopexin in transporting haem to hepatocytes for degradation has been inferred from the high affinity of haemopexin for haem. We have examined this question in primary cultures of chick-embryo and adult rat liver cells. We present here the results of four sets of experiments which indicate that haemopexin retarded haem uptake by hepatocytes in culture. (1) Haem bound to bovine serum albumin is known to repress the activity of delta-aminolaevulinate synthase in chick cultures as indicated by decreased porphyrin accumulation. When haem-albumin was added in the presence of excess puri

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WEXLER, ERIC M., OKSANA BERKOVICH, and SCOTT NAWY. "Role of the low-affinity NGF receptor (p75) in survival of retinal bipolar cells." Visual Neuroscience 15, no. 2 (1998): 211–18. http://dx.doi.org/10.1017/s095252389815201x.

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We have examined the role of neurotrophins in promoting survival of mammalian rod bipolar cells (RBC) in culture. Retinas taken from 8- to 10-day-old Long-Evans rats were dissociated and cultured in media supplemented with either nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), or basic fibroblast growth factor (FGF-2). Survival was measured by the number of cells that were immunoreactive for α-, β-, γ-PKC, a bipolar cell-specific marker. Compared to untreated cultures, CNTF had no effect on RBC survival, while NGF

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Wang, Guang Jian, Hye Joo Chung, Jamie Schnuer, et al. "High Affinity Glutamate Transport in Rat Cortical Neurons in Culture." Molecular Pharmacology 53, no. 1 (1998): 88–96. http://dx.doi.org/10.1124/mol.53.1.88.

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Dissertations / Theses on the topic "Culture affinity"

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Opitz, Lars [Verfasser]. "Development and characterization of affinity- and pseudo-affinity-based methods for cell culture-derived influenza virus capturing / Lars Opitz." Aachen : Shaker, 2010. http://d-nb.info/1081887109/34.

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Snead, Todd Edwin. "Dichotomous Musical Worlds: Interactions between the Musical Lives of Adolescents and School Music-Learning Culture." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/msit_diss/53.

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This ethnographic study investigated the interactions between the musical lives of adolescents and school music-learning culture in a suburban high school. Participants included two music teachers and seven adolescents. Framed within a symbolic interactionist perspective (Blumer, 1969), data were collected via methods consistent with qualitative inquiry, including an innovative data collection technique utilizing music elicitation interviews with adolescents. Findings emerged from the data via thematic analysis (Grbich, 2007). Findings indicate limited interactions between the musical lives of

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Caldera-Noriega, Juan Bautista, and jean_caldera@hotmail com. "A purchaserï¿½s perspective of environmental uncertainty in the international manufacturing products supply chain." RMIT University. Management, 2005. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20060314.162500.

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International companies are increasingly taking advantage of the benefits of globalisation such as continuous improvement in transport and communication technologies that in turn lower transportation costs and decrease logistical difficulties. However, the international environment includes business uncertainty in the form of exchange rate volatility as well as political and economic issues. Moreover, ongoing reduction of trade barriers is reversing the previous tendency to integrate business vertically, shifting international business schemes towards outsourcing all except what the company co

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Tay, Geniesa. "Embracing LOLitics: Popular Culture, Online Political Humor, and Play." Thesis, University of Canterbury. Media and Communication, 2012. http://hdl.handle.net/10092/7091.

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The Internet, and Web 2.0 tools can empower audiences to actively participate in media creation. This allows the production of large quantities of content, both amateur and professional. Online memes, which are extensions of usually citizen-created viral content, are a recent and popular example of this. This thesis examines the participation of ordinary individuals in political culture online through humor creation. It focuses on citizen-made political humor memes as an example of engaged citizen discourse. The memes comprise of photographs of political figures altered either by captions or i

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Briane, Dominique. "Etude de quelques transitions structurales de l'ADN et de la chromatine en relation avec le cycle cellulaire et la différenciation terminale." Paris 13, 1987. http://www.theses.fr/1987PA132017.

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Les changements affectant la structure de la chromatine ont été etudiés dans le foie de rat en régéneration après hépatectomie partielle. Fractionnement de la chromatine par précipitation magnesienne et chromatographie d'affinité met en évidence une augmentation de la fraction de condensée de la chromatine. Modifications de la chromatine ont été étudiées au cours du cycle céllulaire physarum polycephalum, les cinétiques de digestion de la chromatine par la dnase i ont permis de distinguer deux types de structure (de type chromosomique relativement resistante, et une autre plus accessible a l'e

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Vignal, Marie-Noëlle. "Alain-Fournier : culture et création, influences et affinités." Clermont-Ferrand 2, 1994. http://www.theses.fr/1994CLF20066.

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Ce travail vise à dépasser le mythe forgé par la critique hagiographique autour de l'auteur du "Grand Meaulnes". Les échanges épistolaires d'Alain-Fournier avec J. Rivière constituent un fil d'ariane indispensable pour retracer ses années de formation fertiles en rencontres livresques. Parmi ces écrivainss lus souvent avec un enthousiasme juvénile, nous distingons la position centrale occupée par Claudel, Gide, dans lesquels nous avons reconnu les maîtres-penseurs ayant un rôle certain sur sa démarche créatrice et son esthétique romanesque. Nous nous interessons ensuite à la culture artistique

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Lombes, Marc. "Recepteurs mineralocorticoides : caracterisation dans differents modeles experimentaux et purification par chromatographie d'affinite." Paris 6, 1987. http://www.theses.fr/1987PA066495.

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Purification des recepteurs mineralocortiroide (ou recepteur de l'aldosterone de type i) par chromatographie d'affinite. Ces recepteurs ont ete caracterises dans le rein du rat surrenalectomise, le colon humain normal et cancereux et le rein de lapin surrenalectomise. Parmi les agonistes et antagonistes de l'aldosterone etudies, seul un divise en 3 de la dioxycorticosteione presentait les qualites d'affinite et de specificite pour en faire un bon liganol d'affinite. Le gel synthetise avec ce derive s'est avere hautement specifique et selectif et a permis de purifier les recepteurs mineralocort

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Uzcategui, Moncada Maria. "L'influence et représentations de la France au Venezuela : les enjeux autour des relations politiques et culturelles de 1870 à nos jours." Thesis, Lyon 2, 2015. http://www.theses.fr/2015LYO20071.

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À la différence d’autres régions du tiers monde où l’influence de la France a été déterminante et ininterrompue (Moyen-Orient, Maghreb, les anciennes colonies françaises en Afrique centrale ou encore d'autres pays de l’Amérique latine), au Venezuela, il n’y a eu de véritable politique étrangère de coopération culturelle qu’à la fin des années 1950. L'avènement du boom pétrolier pendant l'entre deux guerres a fait décliner l'influence française. À la fin de la Seconde guerre, les diplomates français prennent conscience du potentiel énergétique du Venezuela, observant qu’il est possible de reméd

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Alves, Eduardo Leandro. "Brasil, um país de fé: por que o maior país católico do mundo, também é o maior país pentecostal do mundo?" Faculdades EST, 2012. http://tede.est.edu.br/tede/tde_busca/arquivo.php?codArquivo=386.

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Esta pesquisa busca mostrar que no Brasil houve várias influências religiosas que geraram, a partir da mistura entre os povos e suas religiões, um povo místico bastante voltado para o sobrenatural. Assim, com a chegada dos Missionários Pentecostais em 1910, a Teologia Pentecostal encontrou um terreno fértil, pois a mensagem anunciada apresentava Deus intervindo de forma visível na vida do povo, curando as suas doenças, salvando a sua alma e gerando a esperança de salvação eterna. Sendo assim, investiga-se em que medida a cultura religiosa brasileira, fruto das várias etnias que formaram o que

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Gavériaux, Claire. "Etude de l'interaction entre l'immunoglobuline e et son recepteur de forte affinite : mise au point d'un nouvel essai immunoenzymatique sur cellules, le celisa, importance de la n-glycosylation et de l'activation de la proteine kinase c dans l'expresion fonctionnelle de ce recepteur." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13014.

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Books on the topic "Culture affinity"

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1909-, Bacon Francis, and Sutherland Graham Vivian 1903-, eds. Bacon and Sutherland: Patterns of affinity in British culture of the 1940s. Yale University Press, 2005.

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Petrantoni, Giuseppe. Corpus of Nabataean Aramaic-Greek Inscriptions. Fondazione Università Ca’ Foscari, 2021. http://dx.doi.org/10.30687/978-88-6969-507-0.

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The impact of the Hellenization in the Ancient Near East resulted in a notable presence of Greek koiné language and culture and in the interaction between Greek and Nabataean that conducted inhabitants to engrave inscriptions in public spaces using one of the two languages or both. In this questionably ‘diglossic’ situation, a significant number of Nabataean-Greek inscriptions emerged, showing that the koin was employed by the Nabataeans as a sign of Hellenistic cultural affinity. This book offers a linguistic and philological analysis of fifty-one Nabataean-Greek epigraphic evidences existin

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Löwy, Michael. Redemption and utopia: Jewish libertarian thought in Central Europe : a study in effective affinity. Athlone Press, 1992.

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Löwy, Michael. Redemption and Utopia: Jewish libertarian thought in Central Europe : a study in elective affinity. Athlone Press, 1992.

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Löwy, Michael. Redemption and utopia: Jewish libertarian thought in Central Europe : a study in elective affinity. Stanford University Press, 1992.

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Meli, Marco, ed. Le norme stabilite e infrante. Firenze University Press, 2018. http://dx.doi.org/10.36253/978-88-6453-777-1.

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Le norme stabilite e infrante. Saggi italo-tedeschi in prospettiva linguistica, letteraria e interculturale raccoglie saggi di carattere linguistico-letterario che analizzano aspetti della scrittura di autori quali Vasari e Goethe, forme di comunicazione moderna, e aspetti teorici e normativi del linguaggio. Altri saggi si concentrano sull’opera di autori italiani del Novecento (Pirandello e Atzeni), indagandone la dimensione dell’intertestualità e polifonia dei linguaggi. Vengono inoltre studiati il rapporto tra poesia concreta e poesia visiva, nonché la tecnica espressiva del linguaggio in a

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Lombardi, Sara, ed. Lettere di Margherita Guidacci a Mladen Machiedo. Firenze University Press, 2015. http://dx.doi.org/10.36253/978-88-6655-893-4.

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La raccolta delle Lettere di Margherita Guidacci a Mladen Machiedo rilegge il percorso culturale e poetico dell’autrice alla luce delle affinità con gli scrittori cattolici fiorentini, del legame con la terra dell’originario Mugello, della formazione di anglista, del lavoro critico, della costante fedeltà alla poesia (dagli esordi poetici con La sabbia e l’angelo nel 1946, fino all’ultima prova, Anelli del tempo del 1993). Il volume raccoglie le lettere che tra il 1968 e il 1989 la Guidacci scrisse a Machiedo, poeta, traduttore e insigne italianista croato, a cui la legavano comuni interessi c

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Effective DevOps: Building a Culture of Collaboration, Affinity, and Tooling at Scale. O'Reilly Media, 2016.

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Smith, Abraham. The Bible in African American Culture. Edited by Paul C. Gutjahr. Oxford University Press, 2017. http://dx.doi.org/10.1093/oxfordhb/9780190258849.013.12.

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The chapter explores the motivations for the use of the Christian Bible in distinctive temporal arcs within African American culture. Initially, the chapter acknowledges the oddity of an African American affinity with the Bible because that Bible was deployed to support the enslavement and perpetual exploitation of African descendants in the British colonies that later became the United States. Then, it articulates three reasons for the aforementioned affinity: the availability of the Bible (especially the King James Bible) to provide a language world for personal and collective expression; th

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Roberts, Lee M., and Joanne Miyang Cho. Transnational Encounters between Germany and Korea: Affinity in Culture and Politics Since the 1880s. Palgrave Macmillan, 2018.

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Book chapters on the topic "Culture affinity"

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Behr, Larissa, Pierre Moretti, Fabienne Anton, Cornelia Kasper, and Thomas Scheper. "Selection of High-Producing Cells Via Cell Sorting Using an Affinity Matrix." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_103.

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Yamada, Saichi, Yoshihiro Kamiya, Nozomu Eto, Koji Yamada, Hiroki Murakami, and Tsuyoshi Majima. "Effective Purification of Monoclonal Antibodies by Fast Flow Affinity Chromatography." In Animal Cell Culture and Production of Biologicals. Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3550-4_41.

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Schmidt, Thomas, and Arne Skerra. "The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2447-9_8.

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Gressent, F., J. J. Bono, A. Niebel, H. Canut, J. V. Cullimore, and R. Ranjeva. "Characterization of a High Affinity Binding Site for NodRm Factors in Medicago varia Cell Culture Extracts." In Biological Nitrogen Fixation for the 21st Century. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_94.

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Landgrebe, Daniel, Larissa Behr, Pierre Moretti, et al. "A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture." In Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0884-6_24.

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Tachibana, Hirofumi, Kim Ji-Youn, and Hiroki Murakami. "Generation of Affinity-Variant Antibodies via the Alteration of Glycosylation in Light Chain Effected by Defined Culture Conditions of Human Hybridomas." In Animal Cell Technology: Developments Towards the 21st Century. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0437-1_70.

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García-León, Marta, Elisa Iniesto, and Vicente Rubio. "Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7871-7_21.

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Lübke, Christiane, Jean P. Décieux, Marcel Erlinghagen, and Gert G. Wagner. "Comparing the Risk Attitudes of Internationally Mobile and Non-Mobile Germans." In IMISCOE Research Series. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-67498-4_5.

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AbstractMoving–particularly to a new country–is fraught with risks as migrants leave familiar legal frameworks and cultural institutions behind them. To date, little is known about the psychological determinants of international migration. This chapter helps to fill this gap by analysing data from the first wave of the German Emigration and Remigration Panel Study (GERPS) in combination with data on non-mobile individuals from the German Socio-Economic Panel Study (SOEP). The analyses presented examine whether the risk attitudes of internationally mobile Germans (‘movers’) differ from those of their non-mobile counterparts (‘stayers’). The results show that–with control for key socio-demographic and socio-economic determinants of risk affinity–both emigrants and remigrants report a significantly higher willingness to take risks than stayers. Risk affinity differs within the group of internationally mobile individuals: Emigrants moving to geographically and culturally distant non-European countries report higher risk affinity than those moving to Germany’s neighbouring countries. Emigrants with multiple previous emigration periods are also more willing to take risks. These findings suggest that voluntary emigration from wealthy countries like Germany is only partly a matter of living conditions. Rather, (repeated) emigration seems to be a matter of personality and an expression of a more adventurous lifestyle.

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Wu, Fan. "Cultural affinity of managers of international joint ventures: an experimental study." In Implicit Incentives in International Joint Ventures. Gabler Verlag, 2012. http://dx.doi.org/10.1007/978-3-8349-7076-3_4.

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Monteiro, Juliana, Carla Morais, and Miguel Carvalhais. "NOOA: Maintaining Cultural Identity Through Intergenerational Storytelling and Digital Affinity Spaces." In Interactive Storytelling. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-71027-3_47.

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Conference papers on the topic "Culture affinity"

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Nishi, Masanori, Jun Kobayashi, Masayuki Yamato, et al. "Immobilization of biomolecules onto thermoresponsive culture dishes by affinity binding." In 2006 IEEE International Symposium on MicroNanoMechanical and Human Science. IEEE, 2006. http://dx.doi.org/10.1109/mhs.2006.320283.

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Skorniakova, S. S. "Affinity Of Gender Stereotypes And Political Culture: From Discrimination To Equal Rights." In RPTSS 2017 International Conference on Research Paradigms Transformation in Social Sciences. Cognitive-Crcs, 2018. http://dx.doi.org/10.15405/epsbs.2018.02.133.

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Piovella, F., R. Lombardi, M. Vigotti, A. B. Federici, P. M. Mannucci, and E. Ascari. "VON WILLEBRAND FACTOR MULTIMERS IN CULTURED HUMAN ENDOTHELIAL CELLS: COMPARISON BETWEEN CELLULAR STORAGE POOL AND SUPERNATANT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644100.

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The multimeric composition of von Willebrand factor (vWf) from cultured human endothelial cells (h.e.c.) has been compared with the multimeric composition of vWf from h.e.c. culture supernatant, human normal platelets and plasma. H.e.c. were derived from umbilical cord veins by collagenase digestion, seeded in culture flasks and grown to confluence in TC 199 culture medium, supplemented with 20% foetal calf serum (FCS). At confluency, cells were harvested by rubber policeman, resuspended in 500 |o.l HBSS with protease inhibitors and stored with culture media until assay. H.e.c. and platelet ly

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Zaytsev, Pavel. "Modernism, Publicness, Zombification: Gestalt of "Worker" by E. Junger, And Phenomena of Contemporary Exploitative Culture." In The Public/Private in Modern Civilization, the 22nd Russian Scientific-Practical Conference (with international participation) (Yekaterinburg, April 16-17, 2020). Liberal Arts University – University for Humanities, Yekaterinburg, 2020. http://dx.doi.org/10.35853/ufh-public/private-2020-17.

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The need for researching into ideological sources of contemporary exploitative culture is necessitated by both the outer edge of its interaction with other triggers of modernity, and the inner edge consisting in the answer to the following question: ‘what is an exploitative culture?’. The modernism era gave rise not only to global mass culture, but diverse oppositions of ‘privacy’ and ‘publicity’ categories in their key anthropological images. It seems to us to be no coincidence that exploitative culture is presented by researchers primarily in the anthropological dimension of race and sex. Wh

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Furihata, Kenichi, Diane J. Nugent, Amy L. Bissonette, Elizabeth Vokac, and Thomas J. Kunicki. "PRODUCTION OF HUMAN MONOCLONAL ANTIBODIESSPECIFIC FOR PLATELET MEMBRANE GLYCOPROTEIN IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643705.

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Human monoclonal antibodies specific for platelet membrane glycoproteins (GPs) arepotentially important reagentsfor studies of the immunogenicity of membrane glycoproteins. A human monoclonalautoantibody, 5E5, reactive with plateletGPIIIa has been developed (Nugent, et al.,Blood, 1987, in press). In this report, we describe the production of additional human monoclonal antibodies specific for GPIIIa. Peripheral blood lymphocytes fromone patient with post-transfusion purpur(PTP) and one woman who had delivered an infant with neonatal alloimmune thrombocytopenic purpura (NATP) were used as a sou

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Eguchi, Y., Y. Sakata, M. Matsuda, and K. Kondo. "EFFECT OF THROMBIN-CLEAVAGE OF PRO-UROKINASE ON ITS AFFINITY TO FIBRIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644413.

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Pro-urokinase (PUK) has been reported to bind to fibrin-Sepharose specifically but not to fibrin clot. As thrombin (TH) clotted fibrinogen and cleaved the Arg 156-Phe 157 peptide bond of PUK simultaneously, we tested effect of this cleavage on the binding of PUK to fibrin by using high molecular weight urokinase (HMUK), PUK purified from culture media of human kidney cell (nPUK) and two kinds of recombinant PUK (rPUK) expressed in genetically transformed E. Coli. One rPUK designated as P0 lacks the carbohydrate side chain present in nPUK but has the same amino acids sequence as nPUK does. The

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Sakuragawa, N., S. Saitoh, and K. Takahashi. "INTERACTION BETWEEN CULTURED ENDOTHELIAL CELLS AND ABNORMAL ANTITHROMBIN III "TOYAMA"." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644366.

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Purpose: Abnormal antithrombin III(AT-III)Toyama showed non-affinity to heparin and heparinoid to show loss of immediate antithrombin activity. On the endothelial cells, there are heparinoids including heparan sulfate. We investigated on the interaction between cultured endothelial cells and abnormal AT-III"Toyama" from the viewpoint of antithrombin activity.Materials and methods: (1) Endothelial cell culture:^125I-labelled normal and abnormal AT-III were placed on the washed endothelial cultured cells in 0.2 ml of RPMI-1640 medium for 15 min at 37°C. The medium was suctioned off and the cell

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Brewer, Bryson M., Yandong Gao, Rebecca M. Sappington, and Deyu Li. "Microfluidic Molecular Trap: Probing Extracellular Signaling by Selectively Blocking Exchange of Specific Molecules in Cell-Cell Interactions." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-64489.

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Communication among cell populations is achieved via a wide variety of soluble, extracellular signaling molecules [1]. In order to investigate the role of specific molecules in a cellular process, researchers often utilize in vitro cell culture techniques in which the molecule under question has been removed from the signaling pathway. Traditionally, this has been accomplished by eliminating the gene in the cell that is responsible for coding the targeted ligand/receptor by using modern DNA technology such as gene knockout; however, this process is expensive, time-consuming, and labor intensiv

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Kim, Jong-Hoon, Woon-Hong Yeo, Zhiquan Shu, et al. "Tip Enrichment System for Rapid Screening of Mycobacterium Tuberculosis." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-38403.

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Rapid detection of tuberculosis (TB) has been critically demanded over the last century. To detect TB, numerous methods screening Mycobacterium tuberculosis (MTB) have been developed. However, the methods still have challenges of rapid and specific enrichment of MTB. In this study, we present a novel specific enrichment method of MTB using a microfabricated tip. Through our simulation study, a wavy-shaped microtip is designed to enhance capturing efficiency of bacteria. Using an optimized tip, bacteria are attracted by dielectrophoresis and captured by affinity binding and capillary action. In

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Giddings, J. C. "AN IMMUNORADIOMETRIC ASSAY (IRMA) FOR HUMANTHROMBOMODULIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643963.

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Thrombomodulin was separated from detergent-soluble fractions of human placenta using a combination of DEAE-Sepharose and thrombin-Sepharose chromatography. The final product demonstrated one major band (Mr approximately 100000) and three minor bands (Mr 40000 - 80000) on SDS-polyacrylamide gel electrophoresis. The purified protein markedly enhanced the rate of activation of human protein C by thrombin in the presence of calcium ions. Polyclonal antibodies to the isolated thrombomodulin were raised in rabbits and were shown to inhibit thrombin co-factor activity. Immunofluorescence of fibrobla

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