Academic literature on the topic 'Dead Staining'

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Journal articles on the topic "Dead Staining"

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Volf, D., and R. Jurecic. "Cell staining with DAPI: or dead?" Trends in Genetics 5 (1989): 292. http://dx.doi.org/10.1016/0168-9525(89)90107-8.

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Liu, Yuting, Yuanhong Xu, and Qin Wen. "Carbon dots for staining bacterial dead cells and distinguishing dead/alive bacteria." Analytical Biochemistry 687 (April 2024): 115432. http://dx.doi.org/10.1016/j.ab.2023.115432.

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Li, Yan-Hong, Jia Zeng, Zihao Wang, et al. "Sulfur-Doped Organosilica Nanodots as a Universal Sensor for Ultrafast Live/Dead Cell Discrimination." Biosensors 12, no. 11 (2022): 1000. http://dx.doi.org/10.3390/bios12111000.

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Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much large
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Maibach, Alain, and Pierre Goeldlin de Tiefenau. "Staining Technique for the Integument of Dead and Living Aquatic Larvae (Diptera: Syrphidae)." Entomologia Generalis 17, no. 1 (1992): 69–71. http://dx.doi.org/10.1127/entom.gen/17/1992/69.

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Hiraoka, Yoshinori, and Kazuhide Kimbara. "Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry." Applied and Environmental Microbiology 68, no. 4 (2002): 2031–35. http://dx.doi.org/10.1128/aem.68.4.2031-2035.2002.

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ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and
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PENG, YING-SHIN, SARAH J. LOCKE, MEDHAT E. NASR, T. P. LIU, and MARY ANN MONTAGUE. "Differential staining for live and dead sperm of honey bees." Physiological Entomology 15, no. 2 (1990): 211–17. http://dx.doi.org/10.1111/j.1365-3032.1990.tb00509.x.

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Brenan, M., and C. Parish. "Simultaneous determination of viable and dead lymphocytes by fluorescent staining." Journal of Immunological Methods 102, no. 1 (1987): 147. http://dx.doi.org/10.1016/s0022-1759(87)80020-0.

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Forson, Tetteh-Quarcoo, Ahenkorah, et al. "Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs." Medical Sciences 7, no. 4 (2019): 64. http://dx.doi.org/10.3390/medsci7040064.

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This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S.
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Ciancio, G., A. Pollack, M. A. Taupier, N. L. Block, and G. L. Irvin. "Measurement of cell-cycle phase-specific cell death using Hoechst 33342 and propidium iodide: preservation by ethanol fixation." Journal of Histochemistry & Cytochemistry 36, no. 9 (1988): 1147–52. http://dx.doi.org/10.1177/36.9.2457047.

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We developed a rapid technique for preservation of Hoechst 33342/propidium iodide-stained cells, using ethanol as a fixative. Combined staining with these dyes makes possible analysis of cell-cycle phase-specific cell death. The technique relies on exclusion of propidium iodide from the viable cells, whereas Hoechst stains all of the cells. The bivariate histograms resulting from the flow cytometric analysis contain the equivalent of two single-parameter DNA histograms, one of the living and the other of the dead cell population. Preservation of staining involved addition of 25% ethanol in PBS
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Pasini, Erica M., Denise van den Ierssel, Henri J. Vial, and Clemens HM Kocken. "A novel live-dead staining methodology to study malaria parasite viability." Malaria Journal 12, no. 1 (2013): 190. http://dx.doi.org/10.1186/1475-2875-12-190.

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Dissertations / Theses on the topic "Dead Staining"

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Worp, Everardus Cornelis Johannes van der. "Corneal desiccation in rigid gas permeable contact lens wear time to deal with 3- and 9-o clock staining /." Maastricht : Maastricht : Universitaire Pers ; University Library, Universiteit Maastricht [host], 2008. http://arno.unimaas.nl/show.cgi?fid=14422.

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Book chapters on the topic "Dead Staining"

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Spaepen, Pieter, Sebastian De Boodt, Jean-Marie Aerts, and Jos Vander Sloten. "Digital Image Processing of Live/Dead Staining." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-108-6_21.

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Gantenbein-Ritter, Benjamin, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, and Sibylle Grad. "Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-108-6_14.

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Baloyannis, Stavros J. "Staining of Dead Neurons by the GolgiGolgi MethodGolgi Method in AutopsyAutopsy Material." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2152-2_13.

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Peladan, F., and R. Leitz. "New method for the differential staining of dead and living cells of yeasts and bacteria." In European brewery convention. Oxford University PressOxford, 1991. http://dx.doi.org/10.1093/oso/9780199632831.003.0059.

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Abstract The proposed method uses two fluorescent dyes. It allows to count, in 30’ and with a hight sensitivity (1 cell/100 ml), dead and alive yeasts and bacteria. In other respects, none of the described problems arise and this method could be used all along the beer process.
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Ude, Arinzechukwu, Kaiyven Afi-Leslie, Kelechi Okeke, and Emmanuel Ogbodo. "Trypan Blue Exclusion Assay, Neutral Red, Acridine Orange and Propidium Iodide." In Cytotoxicity [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105699.

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Cytotoxicity and cell viability assessments are very important parameters that are widely used in fundamental research and drug development to determine the safety profile of toxic compounds. These assays measure the degree to which a substance can cause toxic damage to cells or cell death. There are different assays that have been employed to determine the cytotoxicity of substances. These assays either determine enzymatic function, cell viability, mitochondrial activity, lipid metabolism, cell proliferation and/or cell death. These assays entail use of different kinds of dyes such as trypan
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Wahyu, Tri. "Examination for Dry Eyes." In Dry Eye [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98800.

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Dry eye disease (DED) is a multifactorial disease of tears and ocular surface that results in various symptoms with the potential damage to the ocular surface. It can range from mild to severe signs and symptoms and may affect patient’s quality of life. Various techniques and methods have been developed to evaluate DED for initial examination or regular follow up. The simple evaluations that can be performed in clinic include eyelid examination, tear break-up time, and ocular surface stainings; while the advanced ones may require certain devices or laboratory equipment. Careful and thorough ex
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Conference papers on the topic "Dead Staining"

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Fichter, Jennifer, Geddy Hamblen, Chris Janes, Elizabeth J. Summer, Elizabeth J. Summer, and Abigail Mills. "Use of a Methodological Panel to Identify the Source of Problematic Microbial Contamination in an Oil Shale Field." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09019.

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Abstract An oil shale field was found to exhibit classic signs of a heavy microbial burden, including incidences of hydrogen sulfide production, downhole and surface microbially influenced corrosion, downhole pump and surface equipment fouling and fracturing fluid and drilling mud degradation. Over 140 samples, including formation core material, drilling muds, fracturing fluid source waters, production well samples, samples collected from failed pipe surfaces and samples from salt water disposal facilities, were collected in a comprehensive survey. Microbial activity was measured in parallel u
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Seepersad, Balram, Kelvin Ramnath, Shyam Dyal, and Reeza Mohammed. "The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours: A Re-Evaluation of the US Environmental Protection Agency Methodology." In ASME 2002 Engineering Technology Conference on Energy. ASMEDC, 2002. http://dx.doi.org/10.1115/etce2002/ee-29123.

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There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will fac
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Boronyak, Steven M., and W. David Merryman. "Four Week Durability of Combined RF Ablation and Cryo-Anchoring Treatment for Mitral Valve Prolapse." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14198.

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Percutaneous approaches to mitral valve (MV) repair have received a great deal of interest, as they avoid open-chest surgery and are often the only option for patients with significant co-morbidities [1]. One technique currently in development is a combined radiofrequency (RF) ablation and cryo-anchoring catheter, and we recently demonstrated that reduction of MV leaflet surface area due to RF ablation is feasible in the proximity of cryo-anchoring [2]. This reduction of enlarged, diseased MV leaflets is designed to improve leaflet coaptation and reduce mitral regurgitation. However, myocardia
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