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Journal articles on the topic 'Development of UV Spectroscopic Method'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Ganesh, Tiwari* Viral Maheshwari Rohan Shakywanshi Rukhsar khan Dr. Bhoomi Patel. "Development of UV Spectroscopic Method of Dapagliflozin with Some Validation Parameter." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 42–54. https://doi.org/10.5281/zenodo.15317978.

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This research compiles information on the development of analytical methods for estimating Dapagliflozin, which will be valuable for future research on this drug and its impurities. The literature survey covers various analytical methods such as UV, RP-HPLC, HPTLC, and other techniques reported for Dapagliflozin, both alone and in combination with other drugs. The analysis of published data indicates that only a UV spectroscopic method (calibration curve method) has been reported for estimating Dapagliflozin in fixed-dose combination. Each technique is evaluated for its sensitivity, specificit

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*Harini, Uppada, Boddapu Divya, and Bonula Harshini. "DEVELOPMENT AND VALIDATION OF A UV SPECTROSCOPIC METHOD FOR DETERMINATION OF SOTAGLIFLOZIN." World Journal of Pharmaceutical Science and Research 4, no. 3 (2025): 114–20. https://doi.org/10.5281/zenodo.15561449.

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<strong>Background and Objective:</strong><strong> </strong>Sotagliflozin is recommended for patients to reduce the risk of heart failure, type 2 diabetes and chronic kidney diseases. Since there were no analytical methods reported for the estimation of Sotagliflozin till date, the present work aims to develop and validate a UV spectrophotometric method for the evaluation of Sotagliflozin in bulk. <strong>Method: </strong>By using Lab India UV spectrophotometer, the standard solution was scanned against blank in the range of 200-400 nm and the λ max was determined using UV Analyst softw

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Jyothsna, Modugula 1. *. Dr. Manohar Babu. S. 2. Manohar reddy. J. 1. and Sushma R. 1. "A STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION OF CLOZAPINE BY UV SPECTROSCOPIC METHOD IN BULK AND TABLET DOSAGE FORM." Journal of Scientific Research in Pharmacy 8, no. 5 (2019): 48–53. https://doi.org/10.5281/zenodo.2677994.

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<strong><em>ABSTRACT</em></strong> <strong><em>Objective:</em></strong><em> To develop a stability indicating method development and validation of Clozapine by UV spectroscopic method in bulk and tablet dosage form and validate a simple, precise and cost-effective UV spectrophotometric method for the estimation of Clozapine according to the ICH Q2 (R1) guideline. </em> <strong><em>Methods:</em></strong><em> standard Clozapine solution was scanned over UV-Visible range for its wavelength of maximum absorbance. Various calibration standards of Clozapine were prepared and absorbance was recorded

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Jyoti, Chourasiya* Archana Tiwari Aarti Nandwana. "Simultaneous Estimation of Fluconazole and Zinc Pyrithione By Uv Spectroscopy." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1334–49. https://doi.org/10.5281/zenodo.15374756.

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Analytical method development is an approach to select an appropriate assay procedure to determine the composition of various formulations, to prove that particular analytical method is acceptable for use in pharmaceutical laboratories. By keeping all the major questions in mind present methodical evolution of analytical method development and validation is done, in which UV-spectroscopic methods are elaborated. Method development for simultaneous estimation of Zinc Pyrithione and Fluconazole was done in the present study. Literature review revealed that not a single method is reported for the

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Rutvik, Pandya, Kachhiya Heta, N. Desai Mubassira, Patel Dipexa, L. Paradeva Bindiya, and Tandel Jinal. "UV-Spectroscopic Methods Development and Validation for Estimation of Desidustat." Journal of Advances in Pharmaceutical Sciences 2, no. 1 (2024): 42–51. https://doi.org/10.5281/zenodo.10616941.

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<em>Zero order, first order derivative and Difference spectrophotometric methods were developed for routine estimation of Desidustat and validated as per ICH guideline. Linearity obtained for zero order and first order derivative methods from 2-7 </em> <em>g/ml</em><em> with R<sup>2</sup> 0.9965 and 0.9961, respectively and for difference spectrophotometric method 4-12 </em> <em>g/ml</em><em>. with R<sup>2</sup> 0.9978. Standard spiking method for accuracy study was performed and % recovery was found 99.01 to 99.51 % for zero order, 99.49 to 101 % for first order derivative and 99.6 to 10

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Patil, Rupali P., Sandip D. Firke, Md Mojeeb G. Khan, Mohan G. Kalaskar, and Atul A. Shirkhedkar. "UV SPECTROSCOPIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF FAVIPIRAVIR." INDIAN DRUGS 60, no. 08 (2023): 67–71. http://dx.doi.org/10.53879/id.60.08.13094.

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A new, accurate, and easy-to-use UV-spectrophotometry method for analyzing favipiravir in both bulk and tablet forms has been developed. Favipiravir, an antiviral drug, is classified as a modified pyrazine analogue and is also known as 6-fluoro-3-hydroxypyrazine-2-carboxamide. The drug’s concentration was determined by measuring zero-order derivative values at a wavelength of 323 nm. A linear plot was constructed, demonstrating linearity within the concentration range of 4-20 µg mL-1, with an impressive correlation coefficient (r2) of 0.9997 for the zero-order spectrophotometry method. The met

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Pande, Saikat, and Jolly R. Parikh. "Development and Validation of UV- Spectrophotometric Method for estimation of Vancomycin Hydrochloride." Journal of Drug Delivery and Therapeutics 9, no. 3-s (2019): 116–18. http://dx.doi.org/10.22270/jddt.v9i3-s.2959.

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UV-spectrophotometry refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. This method of analysis is gaining importance as it is rapid, simple, precise, less time consuming and highly accurate. The objective of the present investigation was to develop an accurate, rapid and robust method for determination of Vancomycin hydrochloride in pharmaceutical preparations by using UV spectrophotometric method. Vancomycin hydrochloride shows maximum absorbance at a wavelength of 281 nm, which is used for this study. The method provides a linear respon

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Dhobale, Gayatri, Saurabh Kanawade, Rupali Hande, Shankar Dhobale, and Harshal Tare. "Analytical Method Development by Using UV-Spectrophotometer for Estimation of Valsartan in Bulk." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 02 (2024): 854–60. http://dx.doi.org/10.25258/ijpqa.15.2.49.

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In order to accurately determine valsartan (API), this work aims to build a straightforward and exact UV spectroscopic approach. To improve sensitivity and accuracy, the procedure entails methodically optimizing experimental parameters, including solvent choice and wavelength selection. The produced stock solutions were tested for absorbance at 249 nm using a UV spectrophotometer. With %RSD values below 2% for both intra- and inter-day, the devised approach was determined to be quite accurate. At each additional concentration, the approach was shown to be accurate, yielding good drug recoverie

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Kumar HB, Chethan, Bhaskar Kurangi, Moazzim Soudagar, Supriya Chimagave, and Swapnil Patil. "Development and Validation of UV-Spectrophotometric Method for Estimation of Vinpocetine in Marketed Formulation and Nanoformulation." International Journal of Ayurvedic Medicine 14, no. 3 (2023): 717–23. http://dx.doi.org/10.47552/ijam.v14i3.3585.

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A quick, accurate, and cost-effective UV spectroscopy method was developed to estimate the Vinpocetine concentration in bulk, tablet dosage formulations and niosomes formulations, using a solvent ratio of (6:4) methanol: water. According to ICH guidelines, the proposed technique was validated and developed. In spite of linearity, precision, accuracy, specificity, LOD, and LOQ, like parameters were validated by using UV/visible spectroscopy technique to analyze a spiked Vinpocetine solution. The wavelength at which the drug's maximum absorbance peak was obtained at 274 nm and the solvents used

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Gandhi, Santosh Vilaschand, and Barkha Govindlal Kapoor. "Development and Validation of UV Spectroscopic Method for Estimation of Baricitinib." Journal of Drug Delivery and Therapeutics 9, no. 4-s (2019): 488–91. http://dx.doi.org/10.22270/jddt.v9i4-s.3230.

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A simple, sensitive and reproducible spectrophotometric method for the analysis of Baricitinib in pure form and in its dosage form has been developed. Baricitinib is a synthetic antineoplastic and immunomodulating drug. Baricitinib is a selective and reversible Janus kinase 1 (JAK1) and 2 (JAK2) inhibitor. Janus kinases belong to the tyrosine protein kinase family and play an important role in the proinflammatory pathway signalling that is frequently over-activated in autoimmune disorders such as rheumatoid arthritis. Developed method obeyed beer’s law in a concentration range of 10-60 µg/ml w

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Khedkar, Shital, Rutuja Dalvi, Darshana Undre, and Vaibhav Khaladkar. "A Review Paper: Method Development and Validation of Escitalopram." International Journal of Pharmaceutical Research and Applications 10, no. 2 (2025): 575–78. https://doi.org/10.35629/4494-1002575578.

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Escitalopram, a widely used selective serotonin reuptake inhibitor (SSRI), is prescribed for the treatment of depression, anxiety disorders, and other psychiatric conditions. Analytical methods for escitalopram are essential for ensuring its purity, potency, stability, and monitoring its concentration in pharmaceutical formulations and biological matrices. This review summarizes the development and validation of various analytical methods for escitalopram, focusing on chromatographic techniques such as High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-M

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12

Kanchan, Kohli. "DEVELOPMENT OF ULTRAVOILET SPECTROSCOPIC AND THIN LAYER CHROMATOGRAPHY METHOD OF BROCCOLI EXTRACT." iajps,csk publications 03, no. 10 (2016): 1309–214. https://doi.org/10.5281/zenodo.168386.

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Broccoli contains sulforaphane, an isothiocyanate which is formed by the action of myrosinase enzyme on glucoraphanin. This sulforaphane has anticancer properties and can also help in treating many diseases like kidney disorders, skin diseases etc. The present paper highlights the development of UV and TLC method, which will be used in further research. The paper highlights the development of UV method in three media namelyDistilled water, phosphate buffer 7.4 and HCl buffer 2.3. This paper also highlights the Development of TLC method for the identification of sulforaphane in Broccoli extract

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Mallika Adhatrao. "Contemporary Trends in Analytical Spectroscopy and Integrating Qbd Principles for Enhanced Method Development." Journal of Pharma Insights and Research 2, no. 5 (2024): 155–72. http://dx.doi.org/10.69613/tne5y240.

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Analytical spectroscopy has undergone significant advancements in recent years, driven by technological innovations and the demand for more robust, efficient, and reliable methods. The integration of Quality by Design (QbD) principles in spectroscopic method development has emerged as a powerful approach to enhance performance, reliability, and regulatory compliance. Contemporary trends in UV-Visible, Infrared (IR), Near-Infrared (NIR), Raman, Nuclear Magnetic Resonance (NMR), and Mass Spectrometry showcase remarkable progress in sensitivity, selectivity, and applicability. QbD implementation

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Akhil, Sharma* Dev Prakash Dahiya Chinu Kumari Shivani Sharma. "Method Development And Validation Of Febuxostat By The UV Spectroscopy." International Journal in Pharmaceutical Sciences 2, no. 4 (2024): 547–52. https://doi.org/10.5281/zenodo.10967854.

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Febuxostat is a novel non-purine selective xanthine oxidase inhibitor approved by the US Food and Drug Administration (FDA) for the treatment of hyperuricemia in adults with gout. The UV spectroscopic method has been developed for the determination of febuxostat in Dimethylformamide (DMF) at the wavelength range between the 270-400 nm. Febuxostat showed an absorption peak at 317 nm. Linearity ranges were found as 2-10 microgram. Developed method was found to be validated and showed good precision and reproducibility.

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Shelke, Ashwini, Pooja Surwae, Atul R. Bendale, Laxmikany Borse, and Anil G. Jadhav. "Method stability indicating method development and validation for emtricitabina by UV spectroscopic and RP-HPLC methods." International Journal of Pharmaceutical Chemistry and Analysis 9, no. 1 (2022): 10–16. http://dx.doi.org/10.18231/j.ijpca.2022.002.

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Emtricitabine (commonly called FTC, systematic name 2 & 3’-dideoxy-5-fluoro-3-thiacytidine) is a nucleoside reverse-transcriptase inhibitor (NRTI) for the prevention and treatment of HIV infection in adults and children. The UV analysis of the drug was carried out on the 240nm wavelength. A simple, sensitive and accurate RP-HPLC method has been developed& validated for the determination of Emtricitabine in bulk formulation. Present method shows high sensitivity with linearity 10 to 50µg/ml (r2 = 0.9991). Various parameters according to ICH guidelines are followed for validating and tes

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Rajnani, Nikhil, and Dr Nalini Satish Kurup. "Method Development of Methotrexate in Phosphate Buffer Solution by UV-Visible Spectroscopy." International Journal of Trend in Scientific Research and Development Volume-2, Issue-4 (2018): 1213–15. http://dx.doi.org/10.31142/ijtsrd14256.

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BC, Semwal, Tiwari R, Tiwari G, and Babu KA. "UV Spectrophotometric Studies of Ashwagandha, Chamomile and Fever Few Flowers Oil: Method Development and Validation." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 14, no. 04 (2024): 1198–208. https://doi.org/10.25258/ijddt.14.4.37.

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Using the UV-Vis spectrophotometry technique is a simple way to estimate or quantify any pharmacological compounds. Present research focused on development and validation of UV-visible method for feverfew (FFW), chamomile (CMM), and ashwagandha (AGA) flower oils. This method was also applied in conjunction with the UV spectrophotometric analysis of AGA, CMM, and FFW. Method validation parameters revealed that specificity, accuracy, linearity, precision and robustness met the acceptance criteria of ICH standards. It was concluded that developed. UV spectroscopic method proved to be sufficiently

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18

M. Patel, Shivani, and Lalit L. Jha. "SIMULTANEOUS UV METHOD DEVELOPMENT FOR DETERMINATION OF ROTIGOTINE HYDROCHLORIDE AND RASAGILINE MESYLATE." Indian Drugs 60, no. 05 (2023): 73–79. http://dx.doi.org/10.53879/id.60.05.13373.

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A precise, accurate analytical UV spectroscopic method has been developed for simultaneous estimation of rotigotine hydrochloride and rasagiline mesylate by using first order derivative spectroscopy. 50 µg mL-1 solutions of both drugs were scanned in the range of 200-800 nm and the first order overlain spectra of both drugs to check zero crossing points. Rotigotine hydrochloride showed zero crossing point at 239 nm where and rasagiline mesylate showed at 273 nm. Six-point calibration curves shown linearity in 2-12 µg mL-1 concentration range for rotigotine hydrochloride and 10-60 µg mL-1 conce

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Priti Ankush Mahadik, Priti Ankush Mahadik. "Analytical Method Development and Validation for Estimation of Tamsulosin Hydrochloride by UV-Spectroscopic Method." International Journal of Pharmaceutical Research and Applications 10, no. 3 (2025): 287–95. https://doi.org/10.35629/4494-1003287295.

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Tamsulosin Hydrochloride is a medication primarily used in men to treat the symptoms of an enlarged prostate, also known as benign prostatic hyperplasia (BPH). It belongs to a class of drugs called alpha – blocker. A simple, specific, rapid, precise and accurate UV Spectroscopic method have been developed and validated for determination of Tamsulosin Hydrochloride. Drug showed the absorption maxima at 280 nm and was linear for a range of 10 – 50 µg/ml with a correlation coefficient 0f 0. 9944.The validation of the above proposed method was done by carrying out precision and accuracy studies. T

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Attimarad, Mahesh, Katharigatta Narayanaswamy Venugopala, Bandar E. Aldhubiab, et al. "Development of UV Spectrophotometric Procedures for Determination of Amlodipine and Celecoxib in Formulation: Use of Scaling Factor to Improve the Sensitivity." Journal of Spectroscopy 2019 (December 7, 2019): 1–10. http://dx.doi.org/10.1155/2019/8202160.

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FDA has recently approved a new fixed-dose combination of amlodipine besylate (AMD) and celecoxib (COX) for the treatment of hypertension and osteoarthritis. No analytical method has been reported for analysis of these two analytes so far. Hence, to monitor the quality and quantity in the formulation of AMD and COX a simple, accurate, precise, economical, and eco-friendly spectroscopic analytical method has been established. The first method involves the determination of AMD and COX by the first derivative UV spectroscopic method with scaling factor 10. However, the second method was based on

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M. Shah, Sakshi, Ilyas H. Momin, Riya M. Patel, and Shreeraj H. Shah. "DEVELOPMENT OF AN ACCURATE UV SPECTROSCOPIC METHOD FOR TINIDAZOLE IN VAGINAL BUFFERS." INDIAN DRUGS 61, no. 05 (2024): 64–67. http://dx.doi.org/10.53879/id.61.05.14405.

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A precise UV spectroscopic method was developed for accurately quantifying tinidazole (TNZ) using the Shimadzu 1900i UV spectrophotometer. Different sample solutions containing TNZ were scanned across a range of concentration 6 µg mL-1 to 26 µg mL-1 between 200-400 nm, generating overlay TNZ spectra showing an absorption maximum at 318nm in vaginal buffer which is made up of phosphate buffer (PB) 4.2 pH with 2% Tween 80® LR and 4 mL of methanol. The construction of a six-point calibration curve demonstrated the linearity of TNZ within the 6-26 µg mL-1 concentration range. The regression equati

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Helgers, Heribert, Axel Schmidt, Lara Julia Lohmann, et al. "Towards Autonomous Operation by Advanced Process Control—Process Analytical Technology for Continuous Biologics Antibody Manufacturing." Processes 9, no. 1 (2021): 172. http://dx.doi.org/10.3390/pr9010172.

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Continuous manufacturing opens up new operation windows with improved product quality in contrast to documented lot deviations in batch or fed-batch operations. A more sophisticated process control strategy is needed to adjust operation parameters and keep product quality constant during long-term operations. In the present study, the applicability of a combination of spectroscopic methods was evaluated to enable Advanced Process Control (APC) in continuous manufacturing by Process Analytical Technology (PAT). In upstream processing (USP) and aqueous two-phase extraction (ATPE), Raman-, Fourie

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Sasikala, M., S. Mohan, V. Gokilambal, S. Mymoona, and P. Hari. "Validated uv-visible spectroscopic analytical method development and stability studies on oseltamivir." Research Journal of Pharmacy and Technology 13, no. 9 (2020): 4323. http://dx.doi.org/10.5958/0974-360x.2020.00764.7.

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Komal K. Pawar, Syed Shoaib Ali, Chetan M. Jain, and Karna B. Khavane. "Method development and Validation of Catechin hydrate by using UV-Visible Spectroscopy." GSC Biological and Pharmaceutical Sciences 32, no. 1 (2025): 001–6. https://doi.org/10.30574/gscbps.2025.32.1.0161.

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Catechin hydrate, a polyphenolic molecule, is widely present in tea leaves, fruits, and vegetables. The present study aims to develop an innovative, uncomplicated, precise, and reliable UV-visible spectroscopic technique to estimate Catechin Hydrate. The λmax of catechin hydrate in methanol: water (90:10) was 278 nm. The drug exhibits linearity within the range of 2–12 μg/ml, with a R2 value of 0.9991. The accuracy of selected method was assessed using a recovery study at three distinct levels: 80%, 100%, and 120%. The % recovery was determined to be between 99.83% and 101.08%. The minimal % R

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Shilpi, Pathak, and Mishra Pradeep. "Development and validation of UV-spectroscopy based stability indicating different methods for the determination of Canagliflozin." Journal of India Chemical Society Vol. 95, Apr 2018 (2018): 459–66. https://doi.org/10.5281/zenodo.5642615.

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Institute of Pharmaceutical Research, GLA University, 17 Km Stone, NH-2, Mathura-Delhi Road, P.O. Chaumuhan, Mathura-281 406, Uttar Pradesh, India E-mail: shivi13pharma@gmail.com, pmishra51@rediffmail.com <em>Manuscript received 23 February 2018, revised 20 March 2018, accepted 21 March 2018</em> Simple and sensitive spectroscopic methods in UV region and visible region were developed for the estimation of Canagliflozin in its pharmaceutical dosage forms. The development and validation of these methods as a stability indicating assay based on the forced degradation studies. Method A was based

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Shailaja, A., P. Aliveni, M. Saseendra, and Aishwarya x. "Method Development and Validation of Pinaveriumbromide in Bulk form by UV - Visible Spectroscopy." International Journal of Science and Research (IJSR) 13, no. 8 (2024): 1383–87. http://dx.doi.org/10.21275/sr24822115203.

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Vijaya U. Swami, Syed Shoaib Ali, Komal K. Pawar, and Chetan M. Jain. "Spectroscopic method development and validation for simultaneous estimation of neomycin sulphate and clotrimazole from bulk and pharmaceutical dosage form." GSC Biological and Pharmaceutical Sciences 32, no. 1 (2025): 017–24. https://doi.org/10.30574/gscbps.2025.32.1.0176.

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A variety of pharmacological medications are available for the treatment of fungal infections. Spectroscopic methods are very effective for estimating such drugs. The study aimed to develop and validate a simple, sensitive, rapid, and precise UV-visible method for simultaneously estimating neomycin sulphate and clotrimazole in bulk and pharmaceutical dosage form. The λmax of neomycin sulphate and clotrimazole were found to be 244 nm and 263nm. The drug exhibits linearity within the range of 300-1800 ug/ml for neomycin sulphate and 2-12 ug/ml for clotrimazole. The minimal % RSD values indicate

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Banerjee, Joytosh, Renu Solanki, and Badri Prakash Nagori. "Method Development and Validation for Estimation of Eperisone Hydrochloride as API and in Tablet Dosage Form by Two Spectroscopic Methods." ISRN Analytical Chemistry 2013 (September 18, 2013): 1–8. http://dx.doi.org/10.1155/2013/534763.

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Two simple and sensitive spectrophotometric methods have been developed for the determination of eperisone hydrochloride based on its ability to be detected in UV region (Normal UV) and its oxidation using potassium permanganate in alkaline medium (kinetic spectroscopic). The detection was done at 261.40 nm and 603.5 nm. The different experimental parameters affecting the method development were studied and optimized. The initial rate and fixed time method were utilized to construct calibration graph, and 5 minutes and 3 minutes, respectively, were found suitable for the determination of the c

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Nguyen, Thi Thuy Khue, Thanh Tam Nguyen, Thuy Dung Vu, Thi Phuong Thao Pham, Phuong Dung Pham, and Thi Luong Tran. "A Novel UV-VIS Spectroscopic Approach for the Development and Validation of Benzoyl Peroxide Quantification Methods." Journal of Health Sciences 2, no. 6 (2025): 167. https://doi.org/10.59070//jhs020624024.

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Objective: Benzoyl peroxide (BPO) is a widely used active ingredient in acne treatments, recognized for its antibacterial and exfoliating properties. This study aims to quantitatively determine the concentration of BPO in an acne cream using UV-Vis spectrophotometry. Methods: the method involves dissolving the cream in a suitable solvent and measuring its absorbance at a specific wavelength. The UV-Vis technique has demonstrated accuracy and reliability quantifying BPO in commercial formulations. This method is suitable for use in quality control and formulation standardization in the pharmace

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Nguyen, Thi Thuy Khue, Thanh Tam Nguyen, Thuy Dung Vu, Thi Phuong Thao Pham, Phuong Dung Pham, and Thi Luong Tran. "A Novel UV-VIS Spectroscopic Approach for the Development and Validation of Benzoyl Peroxide Quantification Methods." Journal of Health Sciences 2, no. 6 (2025): 167. https://doi.org/10.59070/jhs020624024.

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Objective: Benzoyl peroxide (BPO) is a widely used active ingredient in acne treatments, recognized for its antibacterial and exfoliating properties. This study aims to quantitatively determine the concentration of BPO in an acne cream using UV-Vis spectrophotometry. Methods: the method involves dissolving the cream in a suitable solvent and measuring its absorbance at a specific wavelength. The UV-Vis technique has demonstrated accuracy and reliability quantifying BPO in commercial formulations. This method is suitable for use in quality control and formulation standardization in the pharmace

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Dinesh Singh, D. B. Joshi, and R. S. Bhadauria. "Analytical Method Validation for UV Spectroscopic Assay Method of Trypsin-Chymotrypsin Tablets." Journal of Biomedical and Pharmaceutical Research 12, no. 3 (2023): 50–59. http://dx.doi.org/10.32553/jbpr.v12i3.996.

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It is internationally recognized that validation is necessary in analytical laboratories. The use of validated methods is important for an analytical laboratory to show its qualification and competency. When analytical method is utilized to generate results about the characteristics of drug related samples it is essential that the results are trustworthy. They may be utilized as the basis for decisions relating to administering the drug to patients. Analytical method validation required during drug development and manufacturing and these analytical methods are fit for their intended purpose. T

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Attimarad, Mahesh, Anroop B. Nair, Nagaraja Sreeharsha, Bandar E. Al-Dhubiab, Katharigatta N. Venugopala, and Pottathil Shinu. "Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness." International Journal of Environmental Research and Public Health 18, no. 2 (2021): 448. http://dx.doi.org/10.3390/ijerph18020448.

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The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were

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Attimarad, Mahesh, Anroop B. Nair, Nagaraja Sreeharsha, Bandar E. Al-Dhubiab, Katharigatta N. Venugopala, and Pottathil Shinu. "Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness." International Journal of Environmental Research and Public Health 18, no. 2 (2021): 448. http://dx.doi.org/10.3390/ijerph18020448.

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The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were

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Sonar, Kalpesh V., Prabodh Sapkale, Anil Jadhav, Tushar Deshmukh, Swapnil Patil, and Pallavi Murkute. "DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF LAMIVUDINE IN TABLET DOSAGE FORM." International Journal of Current Pharmaceutical Research 9, no. 6 (2017): 86. http://dx.doi.org/10.22159/ijcpr.2017v9i6.23658.

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Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Lamivudine in tablet dosage form.Methods: The drug is freely soluble in analytical grade water. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade water. The drug obeyed the Beer’s law and showed a good correlation of concentration with absorption which reflects in linearity. The UV spectroscopic method was develop

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Mallikarjuna Rao, N., and D. Gowrisankar. "DEVELOPMENT AND VALIDATION OF NOVEL STABILITY INDICATING UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF CINACALCET HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORMS." INDIAN DRUGS 53, no. 12 (2016): 31–36. http://dx.doi.org/10.53879/id.53.12.10600.

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A novel stability indicating UV spectrophotometric method for the quantitative determination of cinacalcet hydrochloride, a calcimimetic, in bulk and tablet dosage forms was developed in the present work. The parameters linearity, precision, accuracy, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. UV spectroscopic determination was carried out at an absorption maximum of 281 nm using methanol as solvent. In the UV spectroscopic method, linearity over the concentration range of cinacalcet hydrochloride was found to be

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Hanif, Md Abu, Hyokyeong Shin, Danbi Chun, et al. "Development of Highly Ultraviolet-Protective Polypropylene/TiO2 Nonwoven Fiber." Journal of Composites Science 8, no. 3 (2024): 86. http://dx.doi.org/10.3390/jcs8030086.

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In recent decades, there has been a rise in public consciousness of the adverse effects of expanded skin contact with sunlight, particularly the ultraviolet (UV) spectrum. UV radiation causes serious health problems like skin cancer, early aging, erythema, pigmentation, etc., due to contact with the skin. Therefore, the highly efficient UV-protection materials were manufactured using polypropylene and TiO2 (PPTO) through cost-effective and easy methods. The designated 7.5 PPTO and 15 PPTO were prepared, varying the amount of TiO2, as well as without using TiO2 (PPNF), which was also manufactur

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Shounak, Mande*. "A Review Article on Hyphenated Techniques Used in Analytical Method and Development." International Journal of Pharmaceutical Sciences 3, no. 1 (2025): 2407–17. https://doi.org/10.5281/zenodo.14762916.

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The hyphenated techniques refers to the combination or synthesis of distinct analytical methodologies. Spectroscopic techniques are mostly paired with chromatographic techniques. After that, an interphase will allow the mixture's separated components from the chromatographic approach to enter the spectroscopic technique. In GC-MS After separation from gas chromatography, ionization and mass spectrometry are added to MS. analysis and measurement of the mass-to-charge ratios of the ions that the mass spectrometer generates from each analysis. GC and MS can be connected by a membrane separator, j

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Pattanayak, Suman, A. Alekhya Prasanna, Ch Kiranmayi, and K. Padmalatha. "Analytical UV Spectroscopic Method Development and Validation for the Estimation of Mycophenolate Mofetil." Asian Journal of Pharmaceutical Analysis 5, no. 4 (2015): 209. http://dx.doi.org/10.5958/2231-5675.2015.00033.2.

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Mohammad, Afrin Roshanara* Mekala Indu Bhavani A. Elphine Prabahar and Rama Rao Nadendla. "DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF ENALAPRIL MALEATE IN BULK AND PHARMACEUTICAL DOSAGE FORM." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 02 (2019): 4049–53. https://doi.org/10.5281/zenodo.2567493.

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<em>A simple, accurate, sensitive, precise and economical spectroscopic method has been developed and validated for the determination of enalapril maleate in bulk and tablet dosage form. The solvent used is double distilled water for solubilizing purpose. The drug shows the absorption maxima of 211nm. The method obeys Beer’s law in the concentration range of 10-60  μg/mL and exhibited good correlation coefficient (R2=0.9983). The developed method is validated statistically as per ICH guidelines for linearity, precision and accuracy.</em> <strong>Keywords: </strong><em>Enalapril m

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Spasevska, Marija, Jane B. Bogdanov, and Hristina Babunovska. "Development of chromatographic methods for thebaine detection and quantification along with some of related alkaloid derivatives." Macedonian Journal of Chemistry and Chemical Engineering 34, no. 2 (2015): 231. http://dx.doi.org/10.20450/mjcce.2015.685.

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<p class="Body">Thebaine is naturally occurring alkaloid which is of great importance in the pharmaceutical industry and up to now there is not a Pharmacopoeial monograph for this compound. There is a definite need for development of simple procedures for routine quality assessment based on physical and spectroscopic properties (melting point, UV-Vis, IR, specific optical rotation) and even more importantly a need for development of chromatographic methods for determination of purity.The main objective of this work was to develop RP-HPLC-UVD method for quantification of thebaine and also

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M. Sangeetha, A. Swarna Mahalakshmi, and T. Rama Rao. "A review on analytical method development and validation of Ziprasidone HCL by UV spectroscopy in bulk and marketed formulation." IJPAR JOURNAL 13, no. 4 (2024): 806–11. https://doi.org/10.61096/ijpar.v13.iss4.2024.806-811.

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This review article provides a comprehensive overview of the development and validation of a UV-Spectroscopic method for the quantification of Ziprasidone Hydrochloride (HCl) in both bulk and marketed formulations. Ziprasidone HCl, an atypical antipsychotic used in the treatment of schizophrenia and bipolar disorder, requires precise analytical methods for its quantification due to its clinical importance. The study emphasizes the methodological aspects, including the selection of solvents, determination of the maximum absorbance wavelength (λmax), and the preparation of standard solutions. Th

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Chanchala, Mukati, Manocha Nimita, Dubey Archana, and Choudhary Shweta. "Development and Validation of UV Spectroscopic Method for Estimation of Lamivudine in Tablet Dosage Form." International Journal of Pharmaceutical Sciences and Medicine 8, no. 6 (2023): 73–79. http://dx.doi.org/10.47760/ijpsm.2023.v08i06.006.

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It is simple, rapid sensitive precise and specific UV spectrophotometric method for the determination of lamivudine in bulk drug and tablet dosage form were developed and validated. A simple double beam UV spectroptometric method has been developed and validated with different parameter such as linearity, precision .The drug freely soluble in DMF. The drug was identified in term of solubility studies and on the basis of melting point done on melting point apparatus of equiptronics. It showed absorption maxima were determined in DMF. The drug obeyed the beer’s law and sowed a good correlation o

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Jadhav, Rajesh S., and Jagdish S. Bharad. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF HYDROCHLOROTHIAZIDE CONTENT USING UV- SPECTROSCOPIC TECHNIQUE." Journal of Advanced Scientific Research 13, no. 05 (2022): 131–36. http://dx.doi.org/10.55218/jasr.202213515.

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A Simple, definite, informal, rapid, precise and accurate UV Spectrophotometric analytical method have been developed and Validated for estimation of Hydrochlorothiazide formulation drug. Hydrochlorothiazide showed the absorption maxima in at 271.0 nm and was linear for a range of 5µg/ml-25µg/ml with correlation coefficient of 0.9995. The validation for the proposed analytical method was performed by using precision and accuracy-recovery studies. The analytical method showed good Intra precision (Repeatability) with relative standard deviation 0.563% and Inter precision with relative standard

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Naimuddin, Riyazuddin Saiyed, C. Jain Vineet, Dedania Zarana, K. Patel Harshad, and C. Patel Rajendrakumar. "Development and validation of UV spectroscopic Q-absorbance ratio method for zonisamide and aripirazole in synthetic mixture." World Journal of Biology Pharmacy and Health Sciences 13, no. 2 (2023): 266–76. https://doi.org/10.5281/zenodo.8026889.

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A simple, specific, accurate and precise Q-Absorbance ration spectrophotometric method was developed and validated for estimation of Zonisamide and Aripirazole in Synthetic Mixture. Zonisamide and Aripirazole showed and iso-absorptive point at 230.50nm in Distilled water. The second wavelength used was 240.60nm which is λmax of Aripirazole in distilled water. The concentration of the drugs was determined by using ratio of absorbance at iso-absorptive point (λ1 =240.60 nm) and at the λmax of Aripirazole (λ2 =230.50 nm). This method is linear for both drugs; in range

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Hagos, Mulu, Mesfin Redi-Abshiro, Bhagwan Singh Chandravanshi та Estifanos Ele Yaya. "Development of Analytical Methods for Determination of β-Carotene in Pumpkin (Cucurbita maxima) Flesh, Peel, and Seed Powder Samples". International Journal of Analytical Chemistry 2022 (11 лютого 2022): 1–11. http://dx.doi.org/10.1155/2022/9363692.

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Vegetables are consumed worldwide in fresh as well as processed forms. Pumpkin is considered as an important vegetable due to its nutritional values. The objective of this study was to evaluate all the analytical parameters and improve the performance of the methods for the determination of β-carotene in pumpkin flesh, peel, and seed samples using UV-VIS, NIR, and FTIR methods. β-Carotene was measured at 453 nm using the UV-VIS method which showed linear range, 0.1 to 12 µg/mL; R2, 0.999; LOD, 0.034 µg/mL; LOQ, 0.1 µg/mL; RSD, 1.5% to 11%; and percent recovery, 83% to 93%. β-Carotene was also

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R. Xavier Arulappa, M. Jerubin Welsingh, M. Lavanya, T. Sterlin, M. Viji, and P. Karuppasamy. "Development and Validation of UV-Visible spectroscopic method for estimation of Nimesulide in bulk and its pharmaceutical formulation." International Journal of Life Science Research Archive 3, no. 2 (2022): 117–24. http://dx.doi.org/10.53771/ijlsra.2022.3.2.0135.

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The aim of the present work was to develop and validate a simple UV Spectroscopic method for the determination of Nimesulide, which is the inhibitor of prostaglandin synthesis from arachidonic acid and platelet aggregation. It has the ability to exert analgesic and anti-inflammatory actions through various mechanisms (ie, COX independent pathways) and also it inhibits the release of tumour necrosis factor (TNFα) and interleukins, and stops the release of histamine from mast cells and reduce the synthesis of platelet activating factor (PAF) from the basophil cells. The UV visible Spectrophotome

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Sri, A. Navya, K. Shiva Prasad, Hafsa Tahreem, et al. "A novel analytical method development and validation of estimation of meclizine HCL by UV spectroscopic method." International Journal of Multidisciplinary Research and Growth Evaluation 4, no. 3 (2023): 953–56. http://dx.doi.org/10.54660/.ijmrge.2023.4.3.953-956.

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A new simple, accurate, rapid, precise, reproducible and cost-effective spectrophotometric method for the quantitative estimation of Meclizine Hcl. The developed UV spectrophotometric method for the quantitative estimation of meclizine HCl is based on measurement of absorption at maxima wavelength 232nm using Methanol: DMF (40:60). The standard and sample solution were prepared by using Methanol: DMF as a solvent. Quantitative determination of the drug was performed at wavelength range 228-234nm.The linearity was established over the concentration range 5, 10, 15, 20, 25, µg/ml for the meclizi

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Santhosh, Illendula* Thota Sumanjali D.Sandhya N.Lavanya G. Koteswar Rao K.N.V. Rao K. Rajeswar Dutt. "METHOD DEVELOPMENT AND VALIDATION OF AFATINIB IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV-SPECTROSCOPIC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 03 (2018): 1569–75. https://doi.org/10.5281/zenodo.1206381.

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A new simple, accurate, rapid, precise, reproducible and cost effective spectrophotometric method for the quantitative estimation of Afatinib in bulk and pharmaceutical dosage form. The developed visible spectrophotometric method for the quantitative estimation of Afatinib is based on measurement of absorption at maximum wavelength 246 nm using Sodiumcitrate with Water as a solvent. The stock solution of Afatinib was prepared, and subsequent suitable dilution was prepared in distilled water to obtained standard curve. The standard solution of Afatinib shows absorption maxima at 246 nm. The dru

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SanthoshIllendula, A.NavyaSree CH.Ganesh K.Sneha M.Sravya B.Sayujya P.Sandhya V.Shirisha K.N.V. Rao K. RajeswarDutt. "METHOD DEVELOPMENT AND VALIDATION OF AXITINIB IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV-SPECTROSCOPIC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 03 (2019): 6221–27. https://doi.org/10.5281/zenodo.2604225.

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<em>A new simple, accurate, rapid, precise, reproducible and cost effective spectrophotometric method for the quantitative estimation of Axitinib in bulk and pharmaceutical dosage form. The developed visible spectrophotometric method for the quantitative estimation of Axitinib is based on measurement of absorption at maximum wavelength 253 nm using DMF with Water as a solvent. The stock solution of Axitinib was prepared, and subsequent suitable dilution was prepared in distilled water to obtained standard curve. The standard solution of Axitinib shows absorption maxima at 253 nm. The drug obey

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V.Shirisha*, K.Sarika Reddy SK.Akbar J.Vamshi K.Archana Santhosh Illendula K.N.V.Rao K. Rajeswar Dutt. "METHOD DEVELOPMENT AND VALIDATION OF DAPAGLIFLOZIN IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV-SPECTROSCOPIC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 04 (2019): 8017–23. https://doi.org/10.5281/zenodo.2647584.

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<em>A simple, sensitive, highly accurate spectrophotometric method has been developed for the determination of Dapagliflozin in bulk and pharmaceutical tablet dosage form as per ICH Guidelines. The adequate drug solubility and maximum assay sensitivity was found in sodium citrate. The absorbance of Dapagliflozin was measured at 231 nm in the wavelength range of 200‐400 nm. Beer’s law was obeyed in the concentration range of 10-50 μg/mL, in the linearity study regression equation was found to be y =0.2125x+0.0085 & amp; correlation coefficient was found to be 0.999. This method was

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