Relevant bibliographies by topics / DNA extract

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'DNA extract'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "DNA extract"

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Kubota, Y., and H. Takisawa. "Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts." Journal of Cell Biology 123, no. 6 (1993): 1321–31. http://dx.doi.org/10.1083/jcb.123.6.1321.

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Xenopus egg extracts prepared before and after egg activation retain M- and S-phase specific activity, respectively. Staurosporine, a potent inhibitor of protein kinase, converted M-phase extracts into interphase-like extracts that were capable of forming nuclei upon the addition of sperm DNA. The nuclei formed in the staurosporine treated M-phase extract were incapable of replicating DNA, and they were unable to initiate replication upon the addition of S-phase extracts. Furthermore, replication was inhibited when the staurosporine-treated M-phase extract was added in excess to the staurospor
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Skała, Ewa, Przemysław Sitarek, Marek Różalski, et al. "Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots ofRhaponticum carthamoidesagainst Induced Oxidative Stress and DNA Damage in CHO Cells." Oxidative Medicine and Cellular Longevity 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/5753139.

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Rhaponticum carthamoideshas a long tradition of use in Siberian folk medicine. The roots and rhizomes of this species are used in various dietary supplements or nutraceutical preparations to increase energy level or eliminate physical weakness. This is the first report to reveal the protective and DNA repair stimulating abilities ofR. carthamoidesroot extracts in Chinese hamster ovary (CHO) cells exposed to an oxidative agent. Both transformed root extract (TR extract) and extract of soil-grown plant roots (NR extract) may be responsible for stimulating CHO cells to repair oxidatively induced
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Nidugala, Hema, Ashwini Prabhu, Ramakrishna Avadhani, and Ravishankar B. "In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis." Biomedicine 43, no. 4 (2023): 1198–202. http://dx.doi.org/10.51248/.v43i4.2309.

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Introduction and Aim: Cyperus rotundus (L.), commonly known as nutgrass, has been in use in Indian medicine traditional system against several ailments. This investigation was performed to assess the in vitro antineoplastic activity of extracts prepared from water and ethanol with C. rotundus rhizomes. Materials and Methods: Toxic effects induced by the C. rotundus extracts on MCF-7 breast adenocarcinoma cells was evaluated using Sulphorhodamine- B (SRB) Assay, Trypan blue assay, Hoechst nuclear staining assay, Acridine Orange-Ethidium bromide (AO-EB) differential fluorescence staining assay a
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Ibrahim, Farah Wahida, Nurul Amirah Abdul Aziz, Latifah Ibrahim, Nurul Farhana Jufri, and Asmah Hamid. "Zingiber zerumbet (L.) Smith Hexane Crude Extract Caused DNA Damage on Leptospira spp." Sains Malaysiana 50, no. 10 (2021): 3085–94. http://dx.doi.org/10.17576/jsm-2021-5010-21.

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Numerous attempts have been made to control leptospirosis by using chemoprophylaxis, but with limited success. The present study was done to investigate the antileptospiral potential of hexane, ethyl acetate and methanol extracts of Zingiber zerumbet rhizomes. The extracts were assayed for antileptospiral activity using broth microdilution method against Leptospira interrogans (serovar Batavie, Canicola, Australis) and Leptospira biflexa serovar Patoc. The Z. zerumbet hexane extract exhibited antileptospiral activity, with IC50 values of 248 μg/mL against L. interrogans serovar Canicola, IC50
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Neslihan, GULEC, GOK Bahar, INAN Pinar, and ERDUGAN Huseyin. "Determination of antimicrobial activities and DNA protection properties of Scytosiphon lomentaria extracts." GSC Biological and Pharmaceutical Sciences 29, no. 2 (2024): 008–14. https://doi.org/10.5281/zenodo.14738356.

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<em>Scytosiphon lomentaria&nbsp;</em>is a brown algae that has many promising biological activities in medicine as well as being consumed as food in many countries. This study aims to investigate the antimicrobial, antimutagenic and DNA damage protective activity of&nbsp;<em>S. lomentaria</em>&nbsp;by obtaining hexane, methanol and water extracts. To evaluate the antimicrobial effect of the extracts, minimum inhibitory concentration (MIC) tests were performed on both yeasts and bacteria. The antimutagenic activities of the extracts were investigated by the Ames test, and their protective effec
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Kaygısız, Mehmet, and Eda Sönmez Gürer. "Determination of the antimicrobial, antioxidant activities and effects on oxidative DNA damage of extracts from three different Salvia species grown in Turkey." Prospects in Pharmaceutical Sciences 23, no. 1 (2025): 1–8. https://doi.org/10.56782/pps.300.

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Salvia officinalis L., known as medicinal sage, Salvia triloba L., known as Anatolian sage, and Salvia sclarea L., known as clary sage, belong to the Lamiaceae family and are species that typically grow in the Mediterranean region. This study aims to investigate the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) values, antioxidant and antimicrobial activities, total flavonoid and total phenolic content, and protective effects against oxidative DNA damage of extracts obtained from the aerial parts of three sage species — S. officinalis, S. triloba, and
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Ward, Peter, and R. Michael Linden. "A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication." Journal of Virology 74, no. 2 (2000): 744–54. http://dx.doi.org/10.1128/jvi.74.2.744-754.2000.

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ABSTRACT Assays have been described in which duplex adeno-associated virus (AAV) DNA can be replicated in HeLa cell extracts with exogenous AAV Rep protein. These assays appear to mimic the AAV DNA replication that occurs in the cell, including the ability of extracts from adenovirus (Ad)-infected cells to replicate duplex AAV DNA templates more efficiently than extracts from uninfected cells can. We showed previously that the Ad-infected extract was able to support a more processive replication than the uninfected extract. When the Ad single-stranded DNA binding protein (Ad-DBP) was added to
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Rajkumar, Venkatadri, Gunjan Guha, and Rangasamy Ashok Kumar. "Antioxidant and Anti-Cancer Potentials ofRheum emodiRhizome Extracts." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–9. http://dx.doi.org/10.1093/ecam/neq048.

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The objective of this study was to determine antioxidant and cytotoxic efficacies of methanolic and aqueous extracts ofRheum emodiWall. ex Meissn. rhizome. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activities, inhibitory effect on lipid peroxidation and Fe3+reducing antioxidant property have been used to investigate antioxidant properties of the extracts. Cytotoxicity of the extracts was tested on MDA-MB-435S and Hep3B cell lines. Both extracts displayed extensive cytotoxicity to the tested cell lines. The extracts were studied for their ability to protect pBR322 DNA
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Rajesh, Kowti, Joshi Vedamurthy, Kumar Mani Rupesh, et al. "UV rays induced DNA damage: Protection by polyphenols enriched extract of mint leaves." World Journal of Advanced Research and Reviews 8, no. 2 (2020): 152–57. https://doi.org/10.5281/zenodo.4318600.

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DNA of live cells is damaged when exposed to harmful Ultraviolet rays.&nbsp; If the cells are exposed to UV rays exposure time more duration of time, more thymine dimers are formed in the DNA and the greater the risk of an incorrect repair or a missed dimer.&nbsp; To analyze the effect of UV rays Calf thymus DNA and prevention by polyphenol enriched extract of Mint leaves and also its non toxic nature. The above study was done by using submarine gel electrophoresis where UV rays are used to damage the DNA and BHA (400&micro;M) used as positive control.&nbsp;&nbsp; The polyphenol enriched extra
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SHAPIRO, DAVID J., PHILLIP A. SHARP, WALTER W. WAHLI, and MARTHA J. KELLER. "A High-Efficiency HeLa Cell Nuclear Transcription Extract." DNA 7, no. 1 (1988): 47–55. http://dx.doi.org/10.1089/dna.1988.7.47.

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Dissertations / Theses on the topic "DNA extract"

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Hua, Xuequn Helen. "Regulation of initiation of DNA replication in Xenopus egg extract /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1997. http://wwwlib.umi.com/cr/ucsd/fullcit?p9814545.

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Blow, J. J. "The control of eukaryotic DNA replication." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233674.

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One of the major limitations on research into the control of eukaryotic DNA replication has been the lack of any cell-free system that initiates DNA replication in vitro. The first part of the disseration describes the establishment of a eukaryotic system, derived from the activated eggs of the South African clawed toad, Xenopus laevis, that efficiently initiates and completes DNA replication in vitro. Using a variety of biochemical techniques I show that DNA added to the extract in the form of sperm nuclei is efficiently replicated over a period of 4 - 6 hours. Replication of nuclear DNA repr
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Tendwa, Maureen Bilinga. "Investigating effects of aqueous root extract of Mondia whitei on sperm functionality." University of the Western Cape, 2016. http://hdl.handle.net/11394/5547.

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Magister Scientiae (Medical Bioscience) - MSc(MBS)<br>Introduction: Mondia whitei commonly known as "White Ginger" is a highly acclaimed medicinal plant that is extensively used across Africa. M. whitei is used as treatment for sexual dysfunction and is considered to be an aphrodisiac by traditional medicine practitioners. Yet, scientific evidence to support these claims are minimal and those that are published possess ambiguity. To date, only one study reporting the in vitro effect of the aqueous rhizome extract of M. whitei on human sperm motility is available. Therefore, the aim of the stud
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Slenn, Tamara Jeannine. "The Ubiquitin Ligase \(CRL4^{Cdt2}\) Targets Thymine DNA Glycosylase for Destruction during DNA Replication and Repair." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10839.

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The E3 ubiquitin ligase \(CRL4^{Cdt2}\) targets proteins for destruction during DNA replication and following DNA damage (Havens and Walter, 2011). Its substrates contain "PIP degrons" that mediate substrate binding to the processivity factor PCNA at replication forks and damage sites. The resulting PCNA-PIP degron complex forms a docking site for \(CRL4^{Cdt2}\), which ubiquitylates the substrate on chromatin. Several \(CRL4^{Cdt2}\) substrates are known, including Cdt1, multiple CDK inhibitors, Drosophila E2f1, human Set8, S. pombe Spd1, and C. elegans \(Pol\eta\) (Havens and Walter, 2011).
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Kransdorf, Evan Paul. "Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells." VCU Scholars Compass, 2004. http://hdl.handle.net/10156/1685.

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Erasmus, Nicolete. "Investigations on the in vitro effects of aqueous Eurycoma longifolia Jack extract on male reproductive functions." Thesis, University of Western Cape, 2012. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_2238_1375971626.

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<p>Eurycoma longifolia (Tongkat Ali<br>TA) is a Malaysian shrub used to treat various illnesses including male infertility. Considering that TA is also used to improve male fertility and no report&nbsp<br>regarding its safety has been published, this study investigated the effects of a patented, aqueous TA extract on various sperm and testicular functions. Materials and Methods This study&nbsp<br>encompasses two parts (part 1: on spermatozoa<br>part 2: on TM3-Leydig and TM4-Sertoli cells). Part 1: Semen samples of 27 patients and 13 fertile donors were divided into two groups,&nbsp<br>washed a
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Shrestha, Prashanta. "Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3917.

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This thesis reports the viability of E. coli cell extracts prepared using equipment that is both common to biotechnology laboratories and able to process small volume samples and expression of proteins containing unnatural amino acids (UAAs) at higher level using PCR amplified linear DNA templates (LETs) in cell-free protein synthesis (CFPS) system. E. coli-based cell extracts are a vital component of inexpensive and high-yielding CFPS reactions. However, effective preparation of E. coli cell extract is limited to high-pressure homogenizers (French press style or impinge-style) or bead mill ho
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Romanowski, Piotr. "Cell cycle regulation of DNA replication in Xenopus egg extracts." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624760.

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Sun, Li. "Studies of initiation of DNA replication using xenopus egg extracts /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9989761.

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Mason, Rebecca Mary Aglaia. "The rejoining of non-homologous DNA double-strand breaks by mammalian cell free extracts." Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336323.

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Books on the topic "DNA extract"

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Melia, Noelle. Analysis by use of the comet assay, of DNA content and integrity in cells extracted from bladder washing specimens fromtumor and control patients. The Author], 1994.

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-1565?, Castanhoso Miguel de, Bermudez João -1570, and Corrêa, Gaspar, active 16th century, eds. The Portuguese expedition to Abyssinia in 1541-1543 as narrated by Castanhoso: With some contemporary letters, the short account of Bermudez, and certain extracts from Corrêa. Nabu, 2012.

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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Introduction to environmental DNA (eDNA). Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0001.

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Chapter “Introduction to environmental DNA (eDNA)” defines the central concepts of this book. Environmental DNA (eDNA) corresponds to a mixture of genomic DNA from many different organisms found in an environmental sample such as water, soil, or feces. DNA metabarcoding can be defined as the simultaneous DNA-based identification of many taxa found in the same eDNA extract. It is usually based on the analysis of a metabarcode (i.e., a short and taxonomically informative DNA region). Metagenomics refers to the assembly and functional analysis of the different genomes found in an environmental sa
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Sanderson, Russell J. Uracil-DNA glycosylase inhibitor protein: Role of carboxylic acid residues and use for measuring the fidelity of uracil-excision DNA repair synthesis in human cell extracts. 1998.

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Sanderson, Russell J. Uracil-DNA glycosylase inhibitor protein: Role of carboxylic acid residues and use for measuring the fidelity of uracil-excision DNA repair synthesis in human cell extracts. 1998.

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McCurdy, Edward, and Leonardo Da Vinci. Notebooks of Leonardo Da Vinci: Selected Extracts from the Writings of the Renaissance Genius. Sirius international (Editions), 2017.

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Vázquez, Cristian. Támesis y Otros Cuentos. Editorial de la Universidad Nacional de La Plata (EDULP), 2007. http://dx.doi.org/10.35537/10915/15916.

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Cuatro amigos, un día de la primavera, una extraña propuesta y una mujer. Con un lenguaje directo Cristian Vazquez construye un relato atrapante donde cada frase genera la inquietante expectativa que sólo el buen suspenso puede lograr.
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Olhares e práticas em diferentes ambientes de aprendizagem: 1ª jornada de estágio do curso de matemática da UEPA. Editora da Universidade do Estado do Pará - Eduepa, 2020. http://dx.doi.org/10.31792/978-85-8458-04-39.

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O E-book é reúne trabalhos selecionados durante a I Jornada de Estágio do Curso de Licenciatura em Matemática da UEPA. Nesse exercício inicial da prática profissional de ensino os jovens professores aproveitaram a oportunidade para vivenciar diversos aspectos da prática profissional e ainda extrair os conhecimentos que são reproduzidos e compartilhados neste livro. Novas perspectivas metodológicas e reflexões interessantes sobre o papel motivacional na atividade de ensino, são só alguns dos exemplos das contribuições presentes nessa obra.
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Anil, Akhil. Seven Secret Strategies to Launch Successful Startups: Most Effective and Time-tested Strategies Extracted From The DNA of Top Companies like Tesla, Amazon, Uber, Quora & Reddit. Independently published, 2016.

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Taylor, Adam. Memoirs of the Rev. Dan Taylor: Late Pastor of the General Baptist Church, Whitechapel, London, with Extracts from His Diary, Correspondence, and Unpublished Manuscripts. HardPress, 2020.

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Book chapters on the topic "DNA extract"

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Zhang, Yongwei, Uwe Werling, and Winfried Edelmann. "Seamless Ligation Cloning Extract (SLiCE) Cloning Method." In DNA Cloning and Assembly Methods. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-764-8_16.

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Hashimoto, Yoshitami, and Vincenzo Costanzo. "Studying DNA Replication Fork Stability in Xenopus Egg Extract." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-129-1_25.

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Cardinali, Gianluigi, Alessandro Bolano, and Fabrizio Fatichenti. "Rapid Method to Extract DNA from Capsulated Yeast Species." In Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology. Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55758-3_69.

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Bagasra, Omar, Ewen McLean, and Mohammad Saffar. "How to Extract DNA from a Biological Sample at a Crime Scene." In Forensic DNA Analyses Made Simple. CRC Press, 2023. http://dx.doi.org/10.1201/9781003182498-13.

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Pandey, Rajeev Kumar, Deepankar Pratap Singh, Godhi Sudhakar, and Vadlamudi Raghavendra Rao. "Ethanol re-precipitation removes PCR inhibitors from Ancient DNA extract." In Antrocom: Journal of Anthropology, edited by Marco Menicocci and Moreno Tiziani. Gorgias Press, 2010. http://dx.doi.org/10.31826/9781463235413-015.

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Sharma, Bechan, and Nitika Singh. "Phytochemicals as DNA protectants and genotoxicants A. Phytochemicals as genotoxicants. B. Plant extract as DNA protectants. C. Isolated compounds as DNA protectants." In Environmental Damage to DNA and the Protective Effects of Phytochemicals. CRC Press, 2021. http://dx.doi.org/10.1201/9780429342059-7.

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Mourragui, Soufiane M. C., Marco Loog, Mirrelijn van Nee, Mark A. van de Wiel, Marcel J. T. Reinders, and Lodewyk F. A. Wessels. "Percolate: An Exponential Family JIVE Model to Design DNA-Based Predictors of Drug Response." In Lecture Notes in Computer Science. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-29119-7_8.

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AbstractMotivation: Anti-cancer drugs may elicit resistance or sensitivity through mechanisms which involve several genomic layers. Nevertheless, we have demonstrated that gene expression contains most of the predictive capacity compared to the remaining omic data types. Unfortunately, this comes at a price: gene expression biomarkers are often hard to interpret and show poor robustness.Results: To capture the best of both worlds, i.e. the accuracy of gene expression and the robustness of other genomic levels, such as mutations, copy-number or methylation, we developed Percolate, a computation
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Lopez, Bernard, and Jacques Coppey. "Duplex-Duplex Homologous Recombination Catalysed by a Human Nuclear Extract. Involvement in Double-Strand Break Repair." In DNA Repair Mechanisms and Their Biological Implications in Mammalian Cells. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-1327-4_21.

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Hagiya, Masami, and Taiga Hongu. "Models of Gellular Automata." In Natural Computing Series. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-9891-1_21.

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AbstractWe summarize our work on gellular automata, which are cellular automata we intend to implement with gel materials. If cellular automata are implemented as materials, it will become possible to realize smart materials with abilities such as self-organization, pattern formation, and self-repair. Furthermore, it may be possible to make a material that can detect the environment and adapt to it. In this article, we present three models of gellular automata, among which the first two have been proposed previously and the third one is proposed here for the first time. Before presenting the m
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Myrold, David D., Kendall J. Martin, and Nancy J. Ritchie. "Gel purification of soil DNA extracts." In Molecular Microbial Ecology Manual. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0351-0_8.

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Conference papers on the topic "DNA extract"

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Stein, J. L., T. Chaturvedi, T. L. Skovhus, and M. H. Thomsen. "Effect of Antimicrobial Halophilic Plant Extracts on Microbiologically Influenced Corrosion (MIC)." In CONFERENCE 2022. AMPP, 2022. https://doi.org/10.5006/c2022-18089.

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Abstract Offshore oil production is susceptible to internal corrosion, which can occur through microbiologically influenced corrosion (MIC) caused by biofilm-forming sulfate-reducing prokaryotes (SRP). The oil and gas industry relies primarily on biocides and mechanical cleaning to mitigate MIC. Halophytes (salt-tolerant plants) produce a variety of bioactive compounds, some of which have antimicrobial activity. MIC was studied on UNS S31600 stainless steel coupons in flasks with three extract types, from four halophytes, at three different concentrations. Flasks were inoculated batch-wise wit
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Romero, J. M., E. Velázquez, J. L. García Villalobos, M. Amaya, and S. Le Borgne. "Genetic Monitoring of Bacterial Populations in a Seawater Injection System. Identification of Biocide Resistant Bacteria and Study of Their Corrosive Effect." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05483.

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Abstract DNA was extracted from a water sample taken from an offshore seawater injection system. DNA was also extracted from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the Polymerase Chain Reaction (PCR) and bacterial diversity was studied using Denaturing Gel Gradient Electrophoresis (DGGE). The obtained results showed that microbial evaluation was biased by the use of artificial culture media although recommended media were used, indicating that microbiological analysis of waters in industrial systems by culturing methods may n
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Price, Andy. "Novel DNA Extraction and Preservation for Identification of Micro-organisms On-site Using Novel Nucleic Acid Extraction Cards for the Oil and Gas Industry." In CORROSION 2012. NACE International, 2012. https://doi.org/10.5006/c2012-01758.

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Abstract Traditional microbiological methods based on culturing procedures such as the Most Probable Number (MPN) technique have been used for many years to monitor microbial numbers in oilfield samples. However, such methods are far from ideal as it is estimated that only 0.01–10% of all micro-organisms present in environmental samples are culturable by these methods. In addition, without further extensive procedures being carried out, such methods cannot identify the variety of micro-organisms in the sample. Molecular microbiological methods (MMM) negate such problems, providing information
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Sublette, Kerry, Richard Eckert, Dora Ogles, Brett Baldwin, and Katherine Clark. "Molecular Analysis of Aramid Polymer Film Impressions of Corrosion Coupon Biofilms." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-4477.

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Abstract Microbiologically influenced corrosion (MIC) is a serious problem that impacts nearly all industries and exacts a severe toll in terms of operating costs, loss of production, deterioration of capital equipment and the consequences of corrosion related failures. While a proactive step, monitoring for MIC-associated microorganisms is often hindered by the fact that sampling methods may not capture key members of the microbial community involved in MIC (sampling biases) and current reliance on conventional culture-dependent methods may underestimate and oversimplify the problem (cultivat
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Jack, T. R., G. Voordouw, A. Agrawal, J. K. Voordouw, and T. Place. "Microbial Communities Associated with External Corrosion on an Operating Pipeline System." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-3911.

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Abstract Emerging molecular methods based on the analysis of DNA extracted from field samples provide a new opportunity to characterize microbial populations associated with corrosion problems in the oil and gas industry. This paper reports the application of these methods to samples obtained from the excavation of external corrosion sites under disbonded coating on an operating transmission pipeline. Insights into the nature of the microbial communities associated with corrosion products, trapped water, coating and soil will be presented along with an assessment of the potential of this appro
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Lin, Xueju, Neil Sharma, and Crystal Lee. "Development and Validation of In-Field qPCR Methods for Water Microbial Analysis at Oil and Gas Facilities." In CORROSION 2015. NACE International, 2015. https://doi.org/10.5006/c2015-05948.

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Abstract Quantitative real-time polymerase chain reaction (qPCR) is a highly sensitive method for the quantification of microorganisms in natural and engineered environments such as oil and gas facilities. This study developed in-field methods for sample processing and nucleic acid extraction from water samples, the design and validation of qPCR assays targeting Domains Bacteria and Archaea, and 3 physiological groups (iron-reducing bacteria, sulfate-reducing microorganisms (SRM), and methanogens) of corrosion-causing microorganisms. A novel, field-friendly method was successful for use in iso
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Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor
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Matić, Sanja Lj, Nikola Srećković, Jelena Katanić Stanković та Vladimir Mihailović. "IN VIVO PROTEKTIVNI EFEKAT EKSTRAKATA BILJKE Lysimachia vulgaris NA DNK OŠTEĆENJA INDUKOVANA ETIL METANSULFONATOM 2022ЗБОРНИК БИОДИВЕРЗИТЕТ". У XXVII savetovanje o biotehnologiji. University of Kragujevac, Faculty of Agronomy, 2022. http://dx.doi.org/10.46793/sbt27.523m.

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The in vivo genotoxic activity of the aerial part and root methanol extracts of Lysimachia vulgaris L. and ability to protect DNA from ethyl methanesulfonate- induced DNA damage was studied using comet assays in Drosophila melanogaster. Results revealed no significant differences in mean genetic damage index between exposure groups (20, 40, 80 mg mL-1 of Drosophila food) and negative control, indicating a non-genotoxic effect. Combined treatment of extracts and ethyl methanesulfonate showed a significant reduction in DNA damage with a percentage of reduction above 80. These findings suggest th
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Oriano, Martina, Leonardo Terranova, Luca Ruggiero, et al. "What is the best technique to extract bacterial DNA from sputum?" In ERS International Congress 2018 abstracts. European Respiratory Society, 2018. http://dx.doi.org/10.1183/13993003.congress-2018.pa5307.

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Valenta Šobot, Ana, Dunja Drakulic, Djurica Katnic, et al. "Gentiana lutea ROOT EXTRACT CYTOTOXIC CONCENTRATION ACTIVATES APOPTOSIS IN PERIPHERAL BLOOD MONONUCLEAR CELLS." In 8th Workshop Food and Drug Safety and Quality. Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, 2024. http://dx.doi.org/10.46793/8fdsq.pb17vs.

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Gentiana lutea root extract displays protective effects, including activity against oxidative stress, inflammation, and effects on the cardiovascular and central nervous systems. Plant-derived compounds could protect against disease progression by regulating homeostasis and eliminating damaged cells, acting like pro-apoptotic agents. In this work, we aimed to test the impact of cytotoxic concentrations of Gentiana lutea root extract on the activation of apoptosis, a controlled cell death mechanism, in peripheral blood mononuclear cells. Apoptotic DNA degradation was tested by a fragmentation a
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Reports on the topic "DNA extract"

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ศิวรังสรรค์, นภา. การผลิต wavelengthgt 11 DNA และ In vitro packaging mixes เพื่อใช้ในงานพันธุวิศวกรรม. จุฬาลงกรณ์มหาวิทยาลัย, 1993. https://doi.org/10.58837/chula.res.1993.17.

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งานวิจัยนี้มีจุดประสงค์ที่จะผลิต lambda gt 11 DNA จากเชื้อ E. coli BNN 97 โดยการเหนี่ยวนำให้เชื้อเกิด lysis ที่อุณหภูมิ 45 C จากนั้นนำ Bacteriophage lambda gt 11 มาบุกรุกเซลล์ให้อาศัย E. coli Y 1088 จะสามารถขยายจำนวน Bacteriophage ให้มากขึ้นก่อนที่จะทำการแยก lambda gt 11 DNA แบบเสกลเล็ก (Requena 1993) และแบบขยายส่วน (Maniatis 1982) ปริมาณ lambda gt 11 DNA ที่แยกได้จากแต่ละวิธีขึ้นอยู่กับจำนวน pfu ของ lambda gt 11 ที่ใช้ในการบุกรุกเซลล์ให้อาศัย ผลการทดลองพบว่า เมื่อใช้ lambda gt 11 จำนวน 5 x 10[superscript 5] pfu บุกรุกเซลล์ให้อาศัย จะสามารถแยก lambda gt 11 DNA แบบเสกลเล็กได้ทั้งหมดเท่ากับ 7.93
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Author, Not Given. Protocol Development & Detection of Marker DNA Extracted from Thread, Extracted from Thread II, Extracted from Holograms, and Extracted from Torque Seal, and DNA Template & Thread Analysis Report. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/942158.

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Yompakdee, Chulee, and Sittiruk Roytrakul. Molecular target of an anti-cancer compound from leaves of Clausena harmandiana (Pierre). Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.32.

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Clausena harmandiana (Pierre) Guillaumin or Song faa dong (in Thai), is classified in Family Rutaceae. Previous study, a coumarin compound designated CHA-01 was isolated from leave extract of C. harmandiana with inhibitory activity against calcium signaling in a ZDS1 null mutant yeast Saccharomyces cerevisiae (delta zds1). However, not much has been known on biological activity of this coumarin. In the past, some other coumarins were reported to contain anti-cancer activity. The aim of this research was to study molecular mechanism on antiproliferation activity of CHA-01 in Jurkat T cells. The
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Kline, Margaret C., and David L. Duewer. Evaluation of methods for assessing the proportion of single-stranded nuclear DNA in human blood extracts. National Institute of Standards and Technology, 2019. http://dx.doi.org/10.6028/nist.sp.1200-27.

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Lance, Richard, and Xin Guan. Variation in inhibitor effects on qPCR assays and implications for eDNA surveys. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41740.

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Aquatic environmental DNA (eDNA) surveys are sometimes impacted by polymerase chain reaction (PCR) inhibitors. We tested varying concentrations of different inhibitors (humic, phytic, and tannic acids; crude leaf extracts) for impacts on quantitative PCR (qPCR) assays designed for eDNA surveys of bighead and silver carp (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix). We also tested for inhibition by high concentrations of exogenous DNA, hypothesizing that DNA from increasingly closely related species would be increasingly inhibitory. All tested inhibitors impacted qPCR, though on
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Glasscott, Matthew, Johanna Jernberg, Erik Alberts, and Lee Moores. Toward the electrochemical detection of 2,4-dinitroanisole (DNAN) and pentaerythritol tetranitrate (PETN). Engineer Research and Development Center (U.S.), 2022. http://dx.doi.org/10.21079/11681/43826.

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Analytical methods to rapidly detect explosive compounds with high precision are paramount for applications ranging from national security to environmental remediation. This report demonstrates two proof-of-concept electroanalytical methods for the quantification of 2,4-dinitroanisol (DNAN) and pentaerythritol tetranitrate (PETN). For the first time, DNAN reduction was analyzed and compared at a bare graphitic carbon electrode, a polyaniline-modified (PANI) electrode, and a molecularly imprinted polymer (MIP) electrode utilizing PANI to explore the effect of surface-area and preconcentration a
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Abdolmaleki, Kourosh. PR-453-205101-R02 On-Bottom Dynamic Simulation. Pipeline Research Council International, Inc. (PRCI), 2023. http://dx.doi.org/10.55274/r0012257.

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This report presents a number of newly added features to the OBS software relevant to the dynamic simulation of pipelines. The additions include a function to extract on-bottom wave induced velocities and hydrodynamic forces on a given length of pipeline with arbitrary distribution of the nodes; a new dynamic simulation module to perform dynamic simulations for a single pipe node; two new soil models including the Verley and Sotberg for sand as well as a custom soil model as per the approach recommended in DNV-RP-F114. The added soil models are implemented in the dynamic solver, which provide
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Söderberg, Linda, Stefan Palm, Caroline Ek, and Johan Östergren. Fem års DNA-analys av avelsfisk - kompensationsodlad lax och havsöring från åtta älvar. Department of Aquatic Resources, Swedish University of Agricultural Sciences, 2023. http://dx.doi.org/10.54612/a.1tnjl9jc1a.

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För att kompensera för kraftigt minskad naturlig reproduktion orsakad av vattenkraftsutbyggnad sker odling och utsättning av betydande mängder lax och havsöring i ett flertal av våra större vattendrag. Varje år tas nya avelsfiskar in för kramning (sea ranching). I Sverige ska all kompensationsodlad utsatt fisk vara fettfeneklippt. Tidigare märktes även en andel av lax- och havsöringsungarna (smolten) med yttre så kallade Carlinmärken, där inrapporterade återfynd har använts för olika studier och uppföljningar. Av flera olika anledningar har dock dessa märkningar i princip upphört. Som ett alte
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Derbentsev, V., A. Ganchuk, and Володимир Миколайович Соловйов. Cross correlations and multifractal properties of Ukraine stock market. Politecnico di Torino, 2006. http://dx.doi.org/10.31812/0564/1117.

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Recently the statistical characterizations of financial markets based on physics concepts and methods attract considerable attentions. The correlation matrix formalism and concept of multifractality are used to study temporal aspects of the Ukraine Stock Market evolution. Random matrix theory (RMT) is carried out using daily returns of 431 stocks extracted from database time series of prices the First Stock Trade System index (www.kinto.com) for the ten-year period 1997-2006. We find that a majority of the eigenvalues of C fall within the RMT bounds for the eigenvalues of random correlation matr
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Grafi, Gideon, and Brian Larkins. Endoreduplication in Maize Endosperm: An Approach for Increasing Crop Productivity. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575285.bard.

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The focus of this research project is to investigate the role of endoreduplication in maize endosperm development and the extent to which this process contributes to high levels of starch and storage protein synthesis. Although endoreduplication has been widely observed in many cells and tissues, especially those with high levels of metabolic activity, the molecular mechanisms through which the cell cycle is altered to produce consecutive cycles of S-phase without an intervening M-phase are unknown. Our previous research has shown that changes in the expression of several cell cycle regulatory
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