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Dissertations / Theses on the topic 'Domain Studies'

Author: Grafiati

Published: 8 September 2023

Last updated: 31 July 2025

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1

Batra, Sharat. "Magneto-optical studies of domain wall oscillations /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487265555439935.

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2

Parnas, Henrietta. "Binding studies of the SH3D domain of intersectin." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=87005.

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The scaffolding protein intersectin1 is involved in clathrin-mediated endocytosis, apoptosis, signal transduction and regulation of cytoskeletal rearrangements. Thus, understanding the interactions of intersectin1 with other proteins has important consequences for cell biology. It had previously been shown that Cdc42 GTPase-activating protein (CdGAP) is inhibited by intersectin1, resulting in increased actin polymerization and lamellipodia formation. The central basic-rich region of CdGAP has been shown to bind to the intersectin1 SH3D, despite not containing any previously described SH3 bin

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3

Menderin, Nathan. "Studies on the Human Sp1 DNA-Binding Domain." Thesis, University of Exeter, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.507135.

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4

Hardman, C. H. "NMR studies of the A domain of HMG1." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603702.

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This thesis describes the solution structure of the A domain of rat HMG1. First, the A domain from rat HMG1 was subcloned, over-expressed and purified from <I>E. coli</I>. Initial characterisation revealed a mixed population of protein species resulting from both intra- and inter-molecular disulphide bond formation. To produce a suitable sample for NMR spectroscopy the non-conserved cysteine 22 was replaced with serine by site-directed mutagenesis. This mutant protein resembles the wild-type in binding to four-way junctions and short DNA duplexes. CD and NMR spectra suggest no major structural

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5

Edwards, Simon David. "Structural studies of the FERM domain of moesin." Thesis, Birkbeck (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268755.

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6

Berthoumieu, Olivia. "Single molecule studies of seven transmembrane domain proteins." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:ff7ae71d-5481-4523-812b-2128fe32f5fc.

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This work aimed at studying biophysical properties of two membrane proteins, one of potential nanotechnological use, bacteriorhodopsin, and one potential drug target, the NTS1 neurotensin receptor, at the single molecule scale. Bacteriorhodopsin (BR) is the only protein in the purple membrane (PM) of the halophilic organism Halobacterium salinarium. It is a light-driven proton pump converting light into a transmembrane proton gradient through isomerization of its retinal chromophore. Its stability, as well as its photoactivity remaining in dry protein layers, has made BR an attractive material

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7

Williams, Samantha Catherine. "Studies of the domain structure of complement factor B." Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358907.

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8

Ali, M. "Structural studies of Xenopus laevis Quaking protein QUA1 domain." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595447.

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A recent report showed that a specific point mutation of glutamate to glycine (E48G) in the QUA1 domain affects both the oligomerisation and the RNA-binding properties of the murine Quaking homologue (QkI). The mutation has also been shown to be embryonic lethal in mice at around day 10 of gestation. The aims of this thesis were to investigate the structural features of QUA1 domain and explain why E48G mutants fail to dimerize. The first objective was pursued by expression and purification of the <i>Xenopus laevis </i>Quaking homologue (pXqua) QUA1 motif, followed by biophysical characterizati

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9

Pommer, Ansgar J. "Mechanistic studies on the DNase domain of colicin E9." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361425.

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10

Scott, Nerida Robyn. "Folding studies of the glucocorticoid receptor DNA-binding domain." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627279.

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11

Thompson, Elizabeth Claire. "Studies on SET and MYND domain proteins in Drosophila." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612456.

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12

Leung, Adelaine Kwun-Wai. "Structural studies of the spliceosomal U4 snRNP core domain." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614942.

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13

Tran, Huong Jade Thien Thi. "Structural and functional studies of JAB1/MPN domain proteins." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614104.

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14

Purves, Maud. "The D-domain of fibrin : structural and functional studies." Doctoral thesis, University of Cape Town, 1987. http://hdl.handle.net/11427/27202.

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The D-domain of fibrin (ogen) was separated from the parent molecule by plasmin digestion in the presence of calcium and isolated by means of DEAE-anion exchange chromatography followed by gel-filtration in buffer containing 4 M urea. Fluorescent-D-dimer (f-D-dimer) was isolated from a plasmic digest of fibrin clotted in the presence of 2.45 mM dansyl cadaverine, a fluorescent lysine analogue. Fluorescent-D-monomer was a by-product of f-D-dimer purification, the yield being determined by the concentration of dansyl cadaverine. At 2.45 mM f-D-monomer was always present in the digest. The f-D-mo

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15

Wu, Dimao. "Synthetic Studies on Phorboxazole Tail Domain and Amphidinolide C." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354560325.

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16

Schmiedeskamp, Mia Ruth. "NMR studies of the DNA-binding domain of ADR1 /." Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/9208.

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17

Patterson, C. J. F. "Topographic, ultrasonic and diffraction studies of helical antiferromagnets." Thesis, University of Hull, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381882.

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18

Powell, Ryan R. "Outback Nevada| Public Domain and Environmental Challenge." Thesis, University of Nevada, Reno, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10633860.

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<p> With the arrival of Euro-Americans to Nevada, settlers and travelers experienced struggles and opportunities on Nevada’s marginal lands. These lands did not fit well with Euro-American ideas of progress and resource-use throughout the second part of the nineteenth century. After 1848, these marginal lands became part of America’s public domain with little promise for permanent settlements. Between 1860 and 1905, Euro-Americans imposed unsustainable land-uses on Nevada’s marginal lands. Due to increased competition on limited rangelands, federal land managers working for t

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19

Zhang, Min. "Crystallographic studies of the E. coli puta proline dehydrogenase domain /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p1426119.

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20

Walke, Stefan. "Biochemical and structural studies of the spliceosomal snRNP core domain." Thesis, University of Cambridge, 2000. https://www.repository.cam.ac.uk/handle/1810/251736.

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21

Patel, Pryank. "Structural studies of the inner DysF domain of human myoferlin." Thesis, Birkbeck (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479383.

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22

Antoniadou, Eleni. "Studies on the biochemistry of the targetin domain of Lysostaphin." Thesis, University of Nottingham, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411404.

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23

Jones, Gareth. "NMR studies of the DNA-binding domain of B-Myb." Thesis, University of Kent, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270703.

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24

Meng, Ling Guang. "Frequency domain methods for turbogenerator and power system transient studies." Thesis, University of Newcastle Upon Tyne, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308982.

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25

Watson, Martin. "Structural studies on the PX domain of sorting nexin 1." Thesis, University of Sheffield, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.434574.

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26

Joo, Chulmin 1976. "Spectral-domain optical coherence phase microscopy for quantitative biological studies." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43142.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008.<br>Includes bibliographical references.<br>Conventional phase-contrast and differential interference contrast microscopy produce high contrast images of transparent specimens such as cells. However, they do not provide quantitative information or do not have enough sensitivity to detect nanometerlevel structural alterations. We have developed spectral-domain optical coherence phase microscopy (SD-OCPM) for highly sensitive quantitative phase imaging in 3D. This technique employs common-path spectral-

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27

Suckling, Richard John. "Structural and functional studies of [delta]-COP Mu-homology domain." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607739.

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28

Wang, Wooi Koon. "Biophysical and functional studies of SAM Domain of human p73α". Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621141.

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29

Morgan, Hugh P. "Structural and biochemical studies of cold shock domain containing proteins." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/15432.

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This thesis describes a novel DNA microarray method for determining the sequence specificity of single-stranded nucleic acid binding proteins (SNABPs). In <i>Salmonella typhimurium </i>six homologous CSPs (<i>St</i>CspA, <i>St</i>CspB, <i>St</i>CspC, <i>St</i>CspD, <i>St</i>CspE, <i>St</i>CspH) have been identified, although their functions are yet to be clearly elucidated. The novel microarray assay revealed two different types of ss DNA binding preferences for either purine or pyrimidine rich sequences. CspD bound purine (guanine) rich sequences, with the consensus binding sequence, 5’-ACGGg

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30

Sekharan, Monica R. "Structural studies of the cGMP-binding GAF domain of PDE5A /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/8502.

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31

Ito, Yoshimichi. "Frequency Domain Studies on Sampled-Data Systems Using FR-Operators." 京都大学 (Kyoto University), 2011. http://hdl.handle.net/2433/142255.

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32

Benetti, Federico. "Structural studies on the C-terminal domain of human PMCA1b." Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425143.

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Plasma Membrane Calcium ATPases (PMCAs) are P-type pumps involved in calcium homeostasis. Their 3D structures are still unknown but a possible topology has been predicted. PMCAs are predicted to have a cytosolic N-terminal domain, a cytosolic C-terminal domain (regulatory domain), ten transmembrane segments and two cytosolic loops called transduction domain and catalytic domain that connect the 2nd and the 3rd, the 4th and the 5th transmembrane segments respectively. Several mechanisms are responsible of their activation: interaction with Ca2+-calmodulin, interaction with acidic phospholipids

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33

Nanao, Max H. "Structural and computational studies of the potassium channel T1 domain and the ligand binding domain of a glutamate receptor /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3036994.

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34

Lu, Zhisheng. "Structural studies of HIV-1 Vif and its SOCS-box domain." Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/structural-studies-of-hiv1-vif-and-its-socsbox-domain(710df582-c838-4935-b6f6-c023195997c7).html.

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The human immunodeficiency virus type I (HIV-1) is a retrovirus that damages the human immune system, which is suppressed by a cellular factor APOBEC3G in non-permissive cells. The viral infectivity factor (Vif) can induce a poly-ubiquitination degradation of APOBEC3G to counteract the immune response by forming an E3 ubiquitin complex composed of cellular proteins Elongin B (EloB), Elongin C (EloC), Cullin 5 and Ring-Box protein. In this project, we solved the first structure for the Vif SOCS-box and EloBC complex in solution by Nuclear Magnetic Resonance, which shows that the proline-rich mo

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35

Plans, Calafell Vanessa. "Studies on RNF8, a ubiquitin ligase with a RING finger domain." Doctoral thesis, Universitat de Barcelona, 2005. http://hdl.handle.net/10803/1868.

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La ubiquitinació és una modificació postraduccional de proteïnes empleada en la regulació de molts tipus diferents de senyalitzacions cel·lulars i processos biològics. Els enzims responsables d'aquesta modificació postraduccional són les E1 o enzims d'activació de ubiquitines, les E2 o enzims de conjugació de ubiquitines i les E3 o lligases de ubiquitines i poden conjugar una o més ubiquitines sobre la proteïna substrat.<br/>L'heterodimer format per UBC13-UEV te activitat de conjugació de ubiquitines mitjançant enllaços que utilitzen la lisina en posició 63 de la ubiquitina en contes de la lis

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36

Salek, Reza M. "NMR studies of the heat shock protein 90 N-terminal domain." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446807/.

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The Hsp90 based chaperone is a ubiquitous protein-folding system in the cytoplasm of eukaryotes. Several signal transduction systems and cell cycling pathways utilise an interaction with Hsp90 as an essential component. The Hsp90 chaperone is an ATP dependent chaperone, which is active as a dimer. The N- terminal domain of Hsp90 itself has very weak ATPase activity and plays an essential role in the mechanism of dimerisation. This study attempts to elucidate the nucleotide binding effects on the Hsp90 N-terminal domain by NMR. Accomplishing backbone assignments of the apo- and AMP-PNP bound fo

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37

Marigheto, Niusa A. "Time-Domain NMR Studies of the Internal Quality of Horticultural Products." Thesis, University of East Anglia, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485289.

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Nuclear Magnetic Resonance was used to investigate selected fruit with internal defects and at different stages of ripening. This was motivated by the need for an industrial NMR sensor of fruit and vegetable quality capable of operating at typical industrial conveyor speeds. Such a sensor requires the development of NMR protocols capable of determining fruit and vegetable quality in a single-shot manner. It also requires knowledge of which combination of NMR parameters is most sensitive to the quality factor of interest. In this thesis we therefore address this issue by exploring the off-line

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38

Hart, Christopher T. "Functional and Structural Studies of the Anti- MinCD Domain of MinE." Thesis, University of Ottawa (Canada), 2010. http://hdl.handle.net/10393/28643.

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The Min protein system, comprised of MinC, MinD and MinE, functions to ensure that the cytokinetic septum formed during bacterial binary fission is placed at midcell in gram negative bacteria. When bound to ATP, MinD binds to the cell membrane and recruits MinC, forming a complex which inhibits formation of the cell division septum. MinE plays a central role in the regulation of this process by opposing this inhibition through interactions with MinD that displace MinC and site-selectively permit formation of the cell division septum at midcell. However, the amino acid residues of MinE that are

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39

Cheng, Shan Amy. "Structure-function studies of secreted PDZ domain-containing protein 2 (sPDZD2)." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558101.

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40

Dutnall, Robert Nicholas. "Structural and functional studies of a zinc finger DNA-binding domain." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360030.

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41

Wilson, Gillian Mary. "High frequency dielectric studies of cell suspensions using time domain reflectometry." Thesis, Bangor University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305946.

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42

Fowler, S. B. "Folding studies on an immunoglobulin domain using chemical and mechanical methods." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599147.

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Part I of this thesis focuses on the folding and stability of TI 127, the 27<SUP>th</SUP> Ig domain from the I-band of human cardiac titin, using chemical denaturants. The first comprehensive protein engineering analysis of the transition of an immunoglobulin (Ig) I-set domain is presented. Preliminary studies reveal that TI 127 folds via a kinetic intermediate. The transition state shifts to a less native-like state upon mutation, an indication of strain in the hydrophobic core. These two effects make a standard analysis impossible. However, the data can be used to identify critical residues

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43

Ford, M. G. J. "Structural and functional studies on ANTH and EUTH domain-containing proteins." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599114.

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In this work, the crystal structure of the NH<sub>2</sub>-terminal domain of CALM, the ubiquitous isoform of AP180, is presented, both alone and bound to various inositol polyphosphates and phosphatidylinositol-4, 5-bisphosphate (PtdIns(4,5)P<sub>2</sub>). The domain has a novel, all-helical fold which we name the AP180like NH<sub>2</sub>-terminal homology (ANTH) domain. The ligands bind to the surface of the structure, to a lysine-rich motif. This lysine-rich motif was confirmed as the binding site by biochemical means and binding to PtdIns(4,5)P<sub>2</sub> is responsible for recruiting full

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44

鄭珊 and Shan Amy Cheng. "Structure-function studies of secreted PDZ domain-containing protein 2(sPDZD2)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39558101.

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45

Jacks, Amanda Jane. "NMR studies of the starch-binding domain of Aspergillus niger glucoamylase." Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364191.

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46

McIntosh, Pauline Bernadette. "NMR based studies of the DNA-binding domain from B-Myb." Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388299.

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47

Klemm, Juli Dawn. "Structural and biochemical studies of Oct-1 POU domain-DNA interactions." Thesis, Massachusetts Institute of Technology, 1995. http://hdl.handle.net/1721.1/32667.

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48

Shu, Ningcheng. "Structural studies of biotinyl domain of E. coli acetyl-CoA carboxylase." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620559.

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49

McIntosh, Lisa. "Development and characterisation studies of a type III fibronectin domain pair." Thesis, University of Strathclyde, 2014. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=24607.

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This thesis presents an investigation into a 9th-10th type III fibronectin (FN) domain pair (FIII9-10) focused on the characterisation and functional activity of the protein. Initial work attempted to establish whether there was a binding interaction between FIII9-10 and IGFBP-5. Surface plasmon resonance failed to demonstrate an interaction and so thereafter the programme examined the use of FIII9-10 as a protein scaffold for cell adhesion. Surface-induced unfolding of proteins results in a loss of function. To improve the conformational stability of FIII9-10, Pro-Pro pairs were introduced. A

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50

Hanson, Sonya M. "Structural, biochemical and computational studies of TRP channel transmembrane domain modularity." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:328269a9-11c0-4d5b-9cb7-d7433cf4d6c4.

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Transient receptor potential (TRP) channels are expressed throughout the central nervous system and have a unique ability to detect a wide range of stimuli including changes in voltage, temperature, pH, lipid environment, small molecule agonists, and mechanical stress. While it is known that TRP channels contain the same six transmembrane helix (S1-S6), tetrameric architecture as voltage-gated channels, the degree to which functional and structural analogies are relevant remains poorly understood. This thesis describes a multidisciplinary approach toward understanding the structure and functio

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