Relevant bibliographies by topics / Domoic acid

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Domoic acid'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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Journal articles on the topic "Domoic acid"

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Eilers, P., S. Conrad, and S. Hall. "Domoic acid analysis." Toxicon 34, no. 3 (1996): 338. http://dx.doi.org/10.1016/0041-0101(96)81010-5.

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Yao, Y., W. H. Nelson, P. Hargraves, and J. Zhang. "UV Resonance Raman Study of Domoic Acid, a Marine Neurotoxic Amino Acid." Applied Spectroscopy 51, no. 6 (1997): 785–91. http://dx.doi.org/10.1366/0003702971941296.

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Domoic acid, an amino acid neurotoxin, produces a single intense resonance Raman peak observed at 1652 cm−1 from aqueous solution when excited at either 242 or 257 nm. The detection limits for domoic acid in water are found to be well within those concentrations determined to be representative of values in toxic phytoplankton cells. Examination of cells known to contain identified large amounts of domoic acid shows that domoic acid spectra are sufficiently intensely excited to allow detection in the presence of normal phytoplankton cell constituents within the cell. Single cells from species e
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Tasker, R. A. R., B. J. Connell, and S. M. Strain. "Pharmacology of systemically administered domoic acid in mice." Canadian Journal of Physiology and Pharmacology 69, no. 3 (1991): 378–82. http://dx.doi.org/10.1139/y91-057.

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Domoic acid, a structural analogue of kainic acid, has been identified as the toxin that poisoned people who consumed contaminated blue mussels harvested from eastern Prince Edward Island in December of 1987. To investigate the pharmacology of domoic acid in vivo we injected groups of mice with serial dilutions of extracts of contaminated mussels and verified domoic acid concentrations using high performance liquid chromatography. Mice progressed through a series of behavioural changes that were both reproducible and dose-dependent. These behaviours formed the basis of a rating scale that was
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Ramsdell, John, and Frances Gulland. "Domoic Acid Epileptic Disease." Marine Drugs 12, no. 3 (2014): 1185–207. http://dx.doi.org/10.3390/md12031185.

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Kawatsu, Kentaro, Yonekazu Hamano, and Tamao Noguchi. "Determination of Domoic Acid in Japanese Mussels by Enzyme Immunoassay." Journal of AOAC INTERNATIONAL 83, no. 6 (2000): 1384–86. http://dx.doi.org/10.1093/jaoac/83.6.1384.

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Abstract Ten samples of commercial blue mussels (Mytilus edulis) from Japan were analyzed for domoic acid by an indirect competitive enzyme immunoassay (idc–EIA) based on an anti-domoic acid monoclonal antibody. Domoic acid was found in all samples at low concentrations (0.11–1.81 ng/g mussel tissue). The presence of domoic acid was confirmed by liquid chromatography coupled with immunoaffinity chromatography using an anti-domoic acid monoclonal antibody as ligand. To our knowledge, this is the first reported detection of domoic acid, a causative agent of amnesic shellfish poisoning, in Japane
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Mok, Jong-Soo, Ka-Jeong Lee, Ki-Cheol Song, and Ji-Hoe Kim. "Validation of the Analysis of Domoic Acid using High Performance Liquid Chromatography." Korean Journal of Fisheries and Aquatic Sciences 43, no. 4 (2010): 293–97. http://dx.doi.org/10.5657/kfas.2010.43.4.293.

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Silvert, W., and D. V. Subba Rao. "Dynamic Model of the Flux of Domoic Acid, a Neurotoxin, through a Mytilus edulis Population." Canadian Journal of Fisheries and Aquatic Sciences 49, no. 2 (1992): 400–405. http://dx.doi.org/10.1139/f92-045.

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A one-compartment computer simulation model was developed to quantify and characterize the accumulation of the neurotoxin domoic acid in blue mussel (Mytilus edulis) in Cardigan Bay, Prince Edward Island, Canada. Comparison of simulation results with field abundances of Nitzschia pungens f. multiseries Hasle, a diatom implicated in the production of domoic acid, and with measurements of domoic acid within Nitzschia cells indicates that the rate of accumulation of domoic acid in mussel tissue during peak bloom conditions may involve unexpected physiological feedback processes. At extremely high
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Stewart, James E., L. J. Marks, M. W. Gilgan, E. Pfeiffer, and B. M. Zwicker. "Microbial utilization of the neurotoxin domoic acid: blue mussels (Mytilus edulis) and soft shell clams (Mya arenaria) as sources of the microorganisms." Canadian Journal of Microbiology 44, no. 5 (1998): 456–64. http://dx.doi.org/10.1139/w98-028.

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The neurotoxin domoic acid is produced in quantity by the diatom Pseudo-nitzschia multiseries and is released to the environment directly and indirectly via food chains. Presumably there is a mechanism for the biodegradation and disposal of domoic acid and as bacteria are logical candidates for such an activity, a search for bacteria competent to carry out biodegradation of domoic acid was initiated. Extensive trials with a wide variety of bacteria isolated mainly from muds and waters taken from the marine environment showed that the ability to grow on or degrade domoic acid was rare; in fact,
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Wright, Jeffrey L. C., Michael Falk, A. Gavin McInnes, and John A. Walter. "Identification of isodomoic acid D and two new geometrical isomers of domoic acid in toxic mussels." Canadian Journal of Chemistry 68, no. 1 (1990): 22–25. http://dx.doi.org/10.1139/v90-005.

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Isodomoic acids E and F, two new geometrical isomers of the neurotoxin domoic acid, have been found to occur with domoic acid and isodomoic acid D in extracts of toxic mussels. The entire set of geometrical isomers can be prepared in the laboratory by photolysis. Keywords: amnesic shellfish toxin, domoic acid, isodomoic acid, neurotoxin.
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Johannessen, Jan N. "Stability of Domoic Acid in Saline Dosing Solutions." Journal of AOAC INTERNATIONAL 83, no. 2 (2000): 411–12. http://dx.doi.org/10.1093/jaoac/83.2.411.

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Abstract Studies designed to assess the effects of repeated low doses of domoic acid require an assessment of its stability in solution under the conditions used for in vivo studies. The stability of 1 mg/mL solutions of domoic acid in saline, with or without ascorbic acid, was determined for 15 weeks. Solutions were refrigerated, but warmed to room temperature for several hours each working day to simulate conditions of actual use. The solutions of domoic acid showed no evidence of decomposition as evidenced by stability of UV absorbance spectrum, concentration of domoic acid as determined by
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Dissertations / Theses on the topic "Domoic acid"

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Parekh, Punam K. "The Photooxidation of Domoic Acid." FIU Digital Commons, 2012. http://digitalcommons.fiu.edu/etd/770.

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Domoic acid (DA) is a naturally occurring cyanotoxin, which upon ingestion, is responsible for amnesic shellfish poisoning (ASP) in both humans and animals. Produced by the marine diatom, Pseudonitzschia, DA is accumulated by a number of marine organisms including shellfish, clams and mussels which upon consumption can lead to headaches, nausea and seizures. Possessing a variety of functional groups the structure of DA contains three carboxyl groups, a pyrrole ring and a potent conjugated diene region allowing for binding to glutamate receptors in the dorsal hippocampus of the brain causing th
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Fleary-Roberts, Nadia. "Towards the total synthesis of domoic acid and the isodomoic acids." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/towards-the-total-synthesis-of-domoic-acid-and-the-isodomoic-acids(a32fd085-8e09-47b5-b533-ef259b1ae8a2).html.

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Sedehizadeh, Simon. "Towards the total synthesis of domoic acid and the isodomoic acids." Thesis, University of Manchester, 2010. https://www.research.manchester.ac.uk/portal/en/theses/towards-the-total-synthesis-of-domoic-acid-and-the-isodomoic-acids(6a1a5ea8-f5a5-4185-8222-5d3486165ac9).html.

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Silvagni, Paul Anthony. "Comparative pathology and diagnosis of domoic acid toxicity /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.

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Lail, Erin M. "Biogeochemical cycling of domoic acid and its isomers in the ocean /." Electronic version (PDF), 2006. http://dl.uncw.edu/etd/2006/laile/erinlail.pdf.

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Knierim, Tika L. "The photodegradation of domoic acid and the effects of metal chelation /." Electronic version (PDF), 2005. http://dl.uncw.edu/etd/2005/knierimt/tikaknierim.html.

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Wittmaack, Christiana. "Behavioral Criteria for the Diagnosis of Domoic Acid Toxicosis in Zalophus californianus." NSUWorks, 2014. http://nsuworks.nova.edu/occ_stuetd/143.

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Introduction California sea lion (Zalophus californianus) health is severely compromised by domoic acid toxicosis, which occurs in high levels during harmful algal blooms of Pseudonitzschia australis along the coast of California. Current diagnostic protocols are often inconclusive due to a 2-48 hour window of detectability within the urinary, circulatory, and gastric systems (Cook, et al. 2011 and Monte, Pers Comm, 2012). Past studies suggest that Z. californianus, with domoic acid toxicosis, commonly display abnormal behaviors (Goldstein, et al. 2008). However, many of these abnormal behavio
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Vranyac-Tramoundanas, Alexandra, and n/a. "Domoic acid-induced cardiac damage : an in vitro and in vivo investigation." University of Otago. Department of Pharmacology & Toxicology, 2007. http://adt.otago.ac.nz./public/adt-NZDU20071012.143651.

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Cardiovascular pathology is seen in both animals and humans after domoic acid intoxication. Whether this damage is direct (i.e., cardiotoxic) or indirect (i.e., CNS/autonomic seizures) is not known. We have previously shown that acute in vitro domoic acid (0.05-0.25[mu]M; 10 min) treatment of isolated cardiac mitochondria compromises mitochondrial FADH and NAD⁺-linked respiratory control and mitochondrial energetics. Domoic acid was shown to traverse and bind the cellular membrane of H9c2 cardiac myoblasts. However it did not compromise cellular viability as assessed using cell quantification
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Hesp, Blair, and n/a. "In vitro and In vivo investigations of tolerance induction and the role of G-protein coupled kainate receptors." University of Otago. Department of Pharmacology & Toxicology, 2005. http://adt.otago.ac.nz./public/adt-NZDU20070503.150053.

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The excitotoxin domoic acid (DOM) acts at both kainic acid (KA)- and α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA)-sensitive glutamate receptors. Clinical reports suggest that elderly people are hypersensitive to the neurological effects of DOM intoxication. Young, but not aged hippocampal slices which have been preconditioned with low concentrations of DOM or KA exhibit an acute �tolerance� to subsequent high doses of DOM or KA; application of the selective AMPA agonist fluorowillardiine (FW) fails to induce tolerance to excitotoxins. The aim of this study was to further investigate
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Fehling, Johanna. "Diversity, ecology and domoic acid production of Pseudo-nitzschia spp. in Scottish waters." Thesis, Open University, 2004. https://pure.uhi.ac.uk/portal/en/studentthesis/diversity-ecology-and-domoic-acid-production-of-pseudonitzschia-spp-in-scottish-waters(4b83d442-d9f6-4b9b-bc2f-666623b42d0b).html.

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Some diatoms of the genus Pseudo-nitzschia produce the toxin domoic acid (DA). Accumulation of DA in shellfish has led to harvesting closures in western Scottish waters since 1999. This thesis investigated the diversity, ecology and distribution of toxic and non-toxic Pseudo-nitzschia species in western Scottish waters and physiological aspects of growth and toxin production dynamics of P. seriata. The temporal and spatial distribution of phytoplankton was analysed in two separate field studies. 1) Temporal changes were followed by sampling a site in coastal Scottish waters weekly to fortnight
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Books on the topic "Domoic acid"

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Health and Welfare Canada. Canada Diseases Weekly Report. Proceedings of a symposium: Domoic Acid toxicity. Health and Welfare Canada., 1990.

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I, Hynie, and Canada. Health and Welfare Canada., eds. Domoic acid toxicity: Proceedings of a symposium. Dept. of National Health and Welfare, 1989.

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Blanchard, John Robert. Pharmacokinetics of Domoic acid following intravenous administration in rats. National Library of Canada, 1990.

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Blanchard, John Robert. Pharmacokinetics of Domoic acid following intravenous administration in rats. University of Prince Edward Island, 1990.

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Brown, Jennifer Ann. Effects of kainic and domoic acids on the release of glutamate and aspartate from the rat brain synsptosomes. National Library, 1992.

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Brown, Jennifer Ann. Effects of kainic and domoic acids on the release of glutamate and aspartate from rat brain synaptosomes. University of Prince Edward Island, 1992.

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Osis, Vicki. Domoic acid and amnesiac shellfish poisoning: By Vicki Osis. Oregon Sea Grant, Oregon State University, 2003.

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1951-, Wood Anne Michelle, Shapiro Lynda M, Oregon State University. Sea Grant College Program., Oregon Institute of Marine Biology., and United States. National Oceanic and Atmospheric Administration., eds. Domoic acid: Final report of the workshop, Oregon Institute of Marine Biology, February 21-23, 1992. 2nd ed. Oregon Sea Grant, Oregon State University, 1994.

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1951-, Wood Anne Michelle, Shapiro Lynda M, Oregon State University. Sea Grant College Program., Oregon Institute of Marine Biology., and United States. National Oceanic and Atmospheric Administration., eds. Domoic acid: Final report of the workshop, Oregon Institute of Marine Biology, February 21-23, 1992. Oregon Sea Grant, Oregon State University, 1993.

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I, Hynie, Todd, E. C. D. 1939-, Canada Food Directorate, and Laboratory Centre for Disease Control (Canada), eds. Domoic acid toxicity: Proceedings of a symposium, Ottawa, Ontario 11-12 April 1989. Health and Welfare, Canada, 1990.

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Book chapters on the topic "Domoic acid"

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La Barre, Stéphane, Stephen S. Bates, and Michael A. Quilliam. "Domoic Acid." In Outstanding Marine Molecules. Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527681501.ch08.

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Cortinovis, Cristina, Leon J. Spicer, Maria Chiara Perego, Teresa Coccini, and Francesca Caloni. "Domoic Acid." In Handbook of Foodborne Diseases. CRC Press, 2018. http://dx.doi.org/10.1201/b22030-96.

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Kara, Mehtap. "Toxicity of Domoic Acid." In Food Safety. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053358787.11.

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Cases of poisoning caused by seafood are common around the world, especially in developing sea and ocean coastal countries. Domoic acid, which became popular with the mass poisoning case in the 1980s, was actually used in alternative treatment practices. Poisoning caused by domoic acid is called amnesic shellfish poisoning. Although poisoning caused by humans attracts more attention, mass poisoning cases caused by other creatures such as sea lions are also important for the ecosystem. Domoic acid poisoning cases are mostly observed after algal blooms. The effects of acute poisoning are fatal a
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Pérez-Gómez, Anabel, and R. Andrew Tasker. "Domoic Acid as a Neurotoxin." In Handbook of Neurotoxicity. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-15080-7_87.

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Pérez-Gómez, Anabel, and R. Andrew Tasker. "Domoic Acid As a Neurotoxin." In Handbook of Neurotoxicity. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-71519-9_87-1.

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Pérez-Gómez, Anabel, and R. Andrew Tasker. "Domoic Acid as a Neurotoxin." In Handbook of Neurotoxicity. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-5836-4_87.

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Doucette, Tracy A., and R. Andrew Tasker. "Perinatal Domoic Acid as a Neuroteratogen." In Neurotoxin Modeling of Brain Disorders—Life-long Outcomes in Behavioral Teratology. Springer International Publishing, 2015. http://dx.doi.org/10.1007/7854_2015_417.

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Tasker, R. Andrew. "Domoic Acid and Other Amnesic Toxins: Toxicological Profile." In Marine and Freshwater Toxins. Springer Netherlands, 2016. http://dx.doi.org/10.1007/978-94-007-6419-4_21.

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Tasker, R. Andrew. "Domoic Acid and Other Amnesic Toxins: Toxicological Profile." In Marine and Freshwater Toxins. Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-007-6650-1_21-1.

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Villac, M. C., D. L. Roelke, T. A. Villareal, and G. A. Fryxell. "Comparison of two domoic acid-producing diatoms: a review." In Twelfth International Diatom Symposium. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-017-3622-0_23.

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Conference papers on the topic "Domoic acid"

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Aronce, Arvin Ian, Aaron Jason B. De Dios, and Cyrel O. Manlises. "Domoic Acid Detection of Razor Clams through Muscle Tissue via YOLOv8." In 2024 IEEE International Conference on Artificial Intelligence in Engineering and Technology (IICAIET). IEEE, 2024. http://dx.doi.org/10.1109/iicaiet62352.2024.10729982.

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Mahajan, Sonali M., Palak Kundu, S. B. Abrish Aaditya, Shalaka Chaphekar, V. S. Jape, and P. B. Karandikar. "Comparative Study of Prominent Carbon Materials for Capacity Improvement of Lead Acid Battery." In 2024 1st International Conference on Sustainability and Technological Advancements in Engineering Domain (SUSTAINED). IEEE, 2024. https://doi.org/10.1109/sustained63638.2024.11073961.

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Tang, Weijie, Ruiling Gao, Wenan Tan, and Jin Zhang. "InsureTVQA: A Progressive Text-Visual Question Answering Method for Insurance Domain." In 2024 IEEE/ACIS 24th International Conference on Computer and Information Science (ICIS). IEEE, 2024. https://doi.org/10.1109/icis61260.2024.10778381.

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Lee, Hoyong, Hayeon Park, and Chang–Gun Lee. "Adaptive Frequency Cluster-Level Performance Profiler for Multi-Domain Applications." In 2024 IEEE/ACIS 22nd International Conference on Software Engineering Research, Management and Applications (SERA). IEEE, 2024. http://dx.doi.org/10.1109/sera61261.2024.10685562.

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Anand, Vinod, Mobin Salasi, Mandar Risbud, and Rolf Gubner. "Low Cost Development of Flowlines - Selection Criteria of Corrosion Resistant Alloys Flowlines." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-4184.

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Abstract Corrosion resistant alloys (CRA) are often used for well-head equipment and the first length of flowlines, until the application of corrosion inhibited carbon steel becomes a viable choice. The objective of this research is to develop a cost effective and reliable material selection model based on experimental data with the help of Artificial Neural Network (ANN). Experiments were carried out in a jet impingement cell based on the Taguchi's orthogonal array (OA). Each steel specimen was subjected to specific conditions involving a pH range of 3 - 5, chloride concentrations between 1 w
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Masouri, Daryoush, Mehrdad Arastoo, and Mahmoud Zafari. "Investigation of Intergranular Corrosion of Alloy 825 in Sour Service." In CORROSION 2006. NACE International, 2006. https://doi.org/10.5006/c2006-06158.

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Abstract Alloy 825, is a nickel base corrosion & heat resistant alloy. This alloy with austenitic matrix has been stabilized with Ti to prevent over production of chromium carbides. However, heating it to the temperature range between 650-800°C, called the “sensitizing” domain, causes chromium carbide precipitation and prepares the alloy for grain boundary corrosion. The present work deals with a case of intergranular attack observed on a tube of a made of alloy 825. This tube was feeding a heater with fuel gas containing H2S. The combustion of this fuel combined with condensation at the s
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Hendrickson, O. D., E. A. Zvereva, A. V. Zherdev, and B. B. Dzantiev. "IMMUNOCHROMATOGRAPHIC TEST SYSTEM FOR SIMULTANEOUS DETECTION OF PHYCOTOXINS MICROCYSTIN-LR AND DOMOIC ACID." In NOVEL TECHNOLOGIES IN MEDICINE, BIOLOGY, PHARMACOLOGY AND ECOLOGY. Institute of information technology, 2022. http://dx.doi.org/10.47501/978-5-6044060-2-1.52-57.

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An immunochromatographic test system has been developed for the simultaneous detection of two phycotoxins, domoic acid (DA) and microcystin-LR (MC-LR). The assay is implemented in an indirect competitive format using anti-species antibodies labeled with colloidal gold nanoparticles. The instrumental/visual detection limits are 2/80 and 0.05/0.3 ng/mL for DA and MS-LR, respectively; the assay duration is 18 min. The applicability of the developed test system for the determination of phycotoxins in seawater is shown. This work was supported by the Russian Science Foundation (project no. 20-43-07
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Hendrickson, O. D., O. N. Solopova, E. A. Zvereva, A. V. Zherdev, P. G. Sveshnikov, and B. B. Dzantiev. "Lateral flow immune sensor for phycotoxin domoic acid: New reactants for sensitive detection." In PROCEEDINGS OF THE 11TH INTERNATIONAL ADVANCES IN APPLIED PHYSICS AND MATERIALS SCIENCE CONGRESS & EXHIBITION. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0144455.

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Falk, Michael. "The Role Of FT-IR In The Identification Of Domoic Acid, A New Shellfish Toxin." In Intl Conf on Fourier and Computerized Infrared Spectroscopy, edited by David G. Cameron. SPIE, 1989. http://dx.doi.org/10.1117/12.969641.

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O'Connell, Eoin, William B. Lyons, Cormac Sheridan, and Elfed Lewis. "Development of a fibre optic sensor for the detection of harmful algae bloom and in particular domoic acid." In 2007 IEEE Instrumentation & Measurement Technology Conference IMTC 2007. IEEE, 2007. http://dx.doi.org/10.1109/imtc.2007.379024.

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Reports on the topic "Domoic acid"

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Wisniewski, Michael, Samir Droby, John Norelli, Dov Prusky, and Vera Hershkovitz. Genetic and transcriptomic analysis of postharvest decay resistance in Malus sieversii and the identification of pathogenicity effectors in Penicillium expansum. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597928.bard.

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Use of Lqh2 mutants (produced at TAU) and rNav1.2a mutants (produced at the US side) for identifying receptor site-3: Based on the fact that binding of scorpion alpha-toxins is voltage-dependent, which suggests toxin binding at the mobile voltage-sensing region, we analyzed which of the toxin bioactive domains (Core-domain or NC-domain) interacts with the DIV Gating-module of rNav1.2a. This analysis was based on the assumption that the dissociation of toxin mutants upon depolarization would vary from that of the unmodified toxin should the substitutions affect a site of interaction with the ch
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Nafikov, Rafael, Jon Schoonmaker, James M. Reecy, et al. Effects of A17924G Genotypes Associated with Thioesterase Domain of Fatty Acid Synthase and K232A Genotypes of Diacylglycerol Acyltransferase-1 on Milk Fatty Acid Composition in Holstein Dairy Cows. Iowa State University, 2009. http://dx.doi.org/10.31274/ans_air-180814-983.

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Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

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Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TI
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Yalovsky, Shaul, and Julian Schroeder. The function of protein farnesylation in early events of ABA signal transduction in stomatal guard cells of Arabidopsis. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7695873.bard.

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Loss of function mutations in the farnesyltransferase β subunit gene ERA1 (enhanced response to abscisic acid), cause abscisic acid hypersensitivity in seedlings and in guard cells. This results in slowed water loss of plants in response to drought. Farnesyltransferase (PFT) catalyses the attachment of the 15-carbon isoprenoid farnesyl to conserved cysteine residues located in a conserved C-terminal domain designated CaaX box. PFT is a heterodimeric protein comprised of an a and b sununits. The a subunit is shared between PFT and geranylgeranyltransferase-I (PGGTI) which catalyses the attachem
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Young, Erin, Cem Kuscu, Christine Watkins, and Murat Dogan. Using CRISPR Gene Editing to Prevent Accumulation of Lipids in Hepatocytes. University of Tennessee Health Science Center, 2022. http://dx.doi.org/10.21007/com.lsp.2022.0007.

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CRISPR gene editing is a molecular technology that can be used to silence gene expression. In this experiment, genes that are known to play a role in lipid accumulation in hepatocytes were targeted. Specifically, levels of fatty acid transport proteins 2 and 5 (FATP2 & 5) have been shown to be elevated in cases of non-alcoholic fatty liver disease. The goal of this experiment was to reduce expression of these genes by using a dead Cas9 (dCas9) protein with an attached inhibitory domain (KRAB) that acts on the promotor region. When measuring the mRNA expression, it was determined that the l
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Fromm, Hillel, and Joe Poovaiah. Calcium- and Calmodulin-Mediated Regulation of Plant Responses to Stress. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568096.bard.

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We have taken a molecular approach to clone cellular targets of calcium/calmodulin (Ca2+/CaM). A 35S-labeled recombinant CaM was used as a probe to screen various cDNA expression libraries. One of the isolated clones from petunia codes for the enzyme glutamate decarboxylase (GAD) which catalyzes the conversion of glutamate to g-aminobutyric acid (GABA). The activity of plant GAD has been shown to be dramatically enhanced in response to cold and heat shock, anoxia, drought, mechanical manipulations and by exogenous application of the stress phytohormone ABA in wheat roots. We have purified the
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Knight, R. D., and B. A. Kjarsgaard. Comparative pXRF and Lab ICP-ES/MS methods for mineral resource assessment, Northwest Territories. Natural Resources Canada/CMSS/Information Management, 2022. http://dx.doi.org/10.4095/331239.

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The Geological Survey of Canada undertook a mineral resource assessment for a proposed national park in northern Canada (~ 33,500 km2) spanning the transition from boreal forest to barren lands tundra. Bedrock geology of this region is complex and includes the Archean Slave Craton, the Archean and Paleoproterozoic Rae domain of the Churchill Province, the Paleoproterozoic Thelon and Taltson magmatic-tectonic zones, and the Paleoproterozoic East Arm sedimentary basin. The area has variable mineral potential for lode gold, kimberlite-hosted diamonds, VMS, vein uranium and copper, SEDEX, as well
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Rafaeli, Ada, and Russell Jurenka. Molecular Characterization of PBAN G-protein Coupled Receptors in Moth Pest Species: Design of Antagonists. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7593390.bard.

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The proposed research was directed at determining the activation/binding domains and gene regulation of the PBAN-R’s thereby providing information for the design and screening of potential PBAN-R-blockers and to indicate possible ways of preventing the process from proceeding to its completion. Our specific aims included: (1) The identification of the PBAN-R binding domain by a combination of: (a) in silico modeling studies for identifying specific amino-acid side chains that are likely to be involved in binding PBAN with the receptor and; (b) bioassays to verify the modeling studies using mut
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Thanyasrisung, Panida, Aemvika Vittayaprasit, Voravee Hoven, Sugai, Motoyuki, and Oranart Matangkasombut. Rapid detection of mutans streptococci by substrate specific binding of automutanolysin : Final report. Faculty of Dentistry, Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.19.

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Chair-side rapid detection of mutans streptococci is an important aid to clinical dental caries risk assessment. Rapid Streptococcus mutans detection tools are available on the market but there are a small number. Automutanolysin (Aml) is a peptidoglycan hydrolase whose cell wall-binding domain (CWBD) has substrate-specificity towards mutans streptococci. This study aims to develop a rapid detection assay using CWBD conjugated with horseradish peroxidase (HRP). However, the recombinant protein was as insoluble form. Therefore, magnetic nanoparticles were used as an alternative reporter to conj
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Locy, Robert D., Hillel Fromm, Joe H. Cherry, and Narendra K. Singh. Regulation of Arabidopsis Glutamate Decarboxylase in Response to Heat Stress: Modulation of Enzyme Activity and Gene Expression. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7575288.bard.

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Most plants accumulate the nonprotein amino acid, g-aminobutyric acid (GABA), in response to heat stress. GABA is made from glutamate in a reaction catalyzed by glutamate decarboxylase (GAD), an enzyme that has been shown by the Israeli PI to be a calmodulin (CaM) binding protein whose activity is regulated in vitro by calcium and CaM. In Arabidopsis there are at least 5 GAD genes, two isoforms of GAD, GAD1 and GAD2, are known to be expressed, both of which appear to be calmodulin-binding proteins. The role of GABA accumulation in stress tolerance remains unclear, and thus the objectives of th
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