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Journal articles on the topic 'Ethidium bromide. eng'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

González-Villaseñor, Lucia Irene, Amanda M. Burkhoff, Víctor Corces, and Dennis A. Powers. "Characterization of Cloned Mitochondrial DNA from the Teleost Fundulus heteroclitus and its Usefulness as an Interspecies Hybridization Probe." Canadian Journal of Fisheries and Aquatic Sciences 43, no. 10 (1986): 1866–72. http://dx.doi.org/10.1139/f86-231.

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Analysis of mitochondrial DNA endonuclease restriction patterns is a powerful tool for studying related species and variation within species. The ethidium bromide staining technique has limited the number of digestions of mitochondrial DNA per individual. Because 32P-end-labeling also imposes severe limitations, we have resorted to cloning the fish (Fundulus heteroclitus) mitochondrial genome in the lambda replacement vector EMBL-3. The clone was used as a radioactive probe via Southern blotting to detect mitochondrial DNA restriction fragments obtained by multiple restriction endonuclease dig

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2

Iwata, M., D. Myerson, B. Torok-Storb, and R. A. Zager. "An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury." Journal of the American Society of Nephrology 5, no. 6 (1994): 1307–13. http://dx.doi.org/10.1681/asn.v561307.

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It has recently been suggested that endonuclease activation and/or apoptosis, possibly triggered by oxidant stress, are important pathogenetic mechanisms in oxygen deprivation/reoxygenation-induced proximal tubular cell death. To explore this possibility, DNA "laddering," a characteristic feature of these processes, was sought in: (1) postischemic rat kidneys (25- or 40-min arterial clamping; 0, 1, 4, 8, 24, and 48 h and 6 days reflow); (2) posthypoxic isolate rat proximal tubular segments and (3) cultured human kidney proximal tubular cells (HK-2) subjected either to energy depletion plus Ca2

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3

Biggiogera, M., and F. F. Biggiogera. "Ethidium bromide- and propidium iodide-PTA staining of nucleic acids at the electron microscopic level." Journal of Histochemistry & Cytochemistry 37, no. 7 (1989): 1161–66. http://dx.doi.org/10.1177/37.7.2471727.

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Ultra-thin sections of various tissues were stained with ethidium bromide or propidium iodide, two fluorescent markers widely used for quantitation of nucleic acids. The fluorochromes, tested at different concentrations, were then revealed by incubation of the sections with neutralized phosphotungstic acid. We showed that at the electron microscopic level only nucleic acid-containing structures are revealed. Chromatin, nucleolus, and ribosomes appear to be stained by the end-product of the reaction. Furthermore, controls with proteases and nucleases showed that the staining is related to the b

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Rouquette-Loughlin, Corinne, Steven A. Dunham, Michael Kuhn, Jacqueline T. Balthazar, and William M. Shafer. "The NorM Efflux Pump of Neisseria gonorrhoeae and Neisseria meningitidis Recognizes Antimicrobial Cationic Compounds." Journal of Bacteriology 185, no. 3 (2003): 1101–6. http://dx.doi.org/10.1128/jb.185.3.1101-1106.2003.

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ABSTRACT In Neisseria gonorrhoeae and Neisseria meningitidis, we identified a gene that would encode a protein highly similar to NorM of Vibrio parahaemolyticus (Y. Morita et al., Antimicrob. Agents Chemother. 42:1778-1782, 1998). A nonpolar insertional mutation in either the gonococcal or meningococcal norM gene resulted in increased bacterial sensitivity to compounds harboring a quaternary ammonium on an aromatic ring (e.g., ethidium bromide, acriflavine hydrochloride, 2-N-methylellipticinium, and berberine). The presence of point mutations within the −35 region of a putative norM promoter o

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5

Seitz, S., A. V. Schally, S. Gluck, et al. "Effective treatment of triple-negative breast cancer with targeted cytotoxic somatostatin analogue AN-162 (AEZS-124)." Journal of Clinical Oncology 27, no. 15_suppl (2009): 619. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.619.

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619 Background: Triple negative breast cancers (TNBC) represent a distinct subtype of breast cancer being negative to ER, PR and HER2 and are associated with poor prognosis. Limited systemic treatment options exist for TNBC. TNBC cells express somatostatin receptors (SSTR). Therefore, to investigate preclinical characteristics of TNBC we used a novel targeted cytotoxic somatostatin analogue AN-162 containing doxorubicin (DOX) which binds to the subtypes 2, 3 and 5 of SSTR. Methods: The expression of SSTR in HCC 1806 human TNBC cell line was detected by RT-PCR. Cytotoxic effect of AN-162 in vit

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Almasan, A., and N. C. Mishra. "Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa." Genetics 120, no. 4 (1988): 935–45. http://dx.doi.org/10.1093/genetics/120.4.935.

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Abstract An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The deletion endpoints are located in the second intron of the ND5 gene, and in a sequence 250 nucleotides upstream of the co2 gene. The recombination has taken place between two nine nucleotide rep

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Shaker, O., H. Aboul Fadl, and M. K. El Hatw. "Prevalence of hepatitis C virus RNA in patients with chronic renal diseases." Eastern Mediterranean Health Journal 4, no. 3 (1998): 548–53. http://dx.doi.org/10.26719/1998.4.3.548.

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The prevalence of hepatitis C virus [HCV] RNA in 80 patients with chronic renal diseases was determined. Two sets of primers from the non-coding region of the hepatitis C virus were used. The products [188 bp] amplified by polymerase chain reaction were visualized by 2% agarose gel electrophoresis stained with ethidium bromide. The patients were classified into four groups. Group I comprised 40 adult patients with end-stage renal disease, 31 of whom were positive for HCV-RNA [77.5%] ; group II, 22 children with glomerulopathies, 15 of whom were positive [68.2%] ; group III, 9 children with chr

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Tsuge, Kenji, Yuichiro Ohata, and Makoto Shoda. "Gene yerP, Involved in Surfactin Self-Resistance in Bacillus subtilis." Antimicrobial Agents and Chemotherapy 45, no. 12 (2001): 3566–73. http://dx.doi.org/10.1128/aac.45.12.3566-3573.2001.

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ABSTRACT Surfactin is a cyclic lipopeptide biosurfactant. Transposon mutagenesis was performed in Bacillus subtilis strain 168, and a surfactin-susceptible mutant, strain 801, was isolated. Analysis of the region of insertion revealed that yerPwas the determinant of surfactin self-resistance. YerP had homology with the resistance, nodulation, and cell division (RND) family proton motive force-dependent efflux pumps only characterized in gram-negative strains. The yerP-deficient strain 802, in which the internal region of the yerP gene of B.subtilis strain 168 was deleted, showed susceptibility

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9

Jakeway, S. C., and U. J. Krull. "Consideration of end effects of DNA hybridization in selection of fluorescent dyes for development of optical biosensors." Canadian Journal of Chemistry 77, no. 12 (1999): 2083–87. http://dx.doi.org/10.1139/v99-200.

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Intercalating fluorescent dyes are in widespread use to detect the presence of double-stranded DNA. Applications include the development of biosensors that rely on the attachment ("tethering") of a dye molecule by a short hydrocarbon chain to the terminus of a strand of DNA so that dye is continuously available and the biosensor is fully reversible. Double strands of DNA have end effects that limit the stability of hybridization and dye intercalation near the termini of the duplexes. Therefore, the selection of the dye must be based on consideration of spectroscopic properties and also issues

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10

Nagy, Lajos, Romeo E. Morales, Martin Beinborn, Peter Vattay, and Sandor Szabo. "Investigation of gastroprotective compounds at subcellular level in isolated gastric mucosal cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 279, no. 6 (2000): G1201—G1208. http://dx.doi.org/10.1152/ajpgi.2000.279.6.g1201.

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We tested the hypothesis that recognized gastroprotective agents exert direct protection against ethanol-induced injury in isolated rat gastric mucosal cells in vitro. If protection exists, we also wanted to identify subcellular targets in the reversible and/or irreversible stages of cell injury. Ethanol-induced cell injury was quantified by measuring plasma membrane leakage (trypan blue exclusion and lactate dehydrogenase release), mitochondrial integrity (succinic dehydrogenase), and nuclear damage (ethidium bromide-DNA fluorescence). Initial cell viability and responsiveness were estimated

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Vo-Quang, Tuyen, Yves Malpiece, Dominique Buffard, P. Alexandre Kaminski, Dominique Vidal, and A. Donny Strosberg. "Rapid large-scale purification of plasmid DNA by medium or low pressure gel filtration. Application: construction of thermoamplifiable expression vectors." Bioscience Reports 5, no. 2 (1985): 101–11. http://dx.doi.org/10.1007/bf01117056.

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This paper describes a new method of lasmid DNA purification which is fast and reliable enough for most purposes in recombinant DNA technology. The present method does not require the use of toxic chemicals such as phenol or ethidium bromide, costly ultra-centrifugation procedures or other processes which can modify the supercoiled structure of the plasmids, such as adsorption on glass fiber. This method is based on the principle of gel filtration chromatography, at low pressure (1 bar) or medium pressure (between 5 and 10 bars), using Sephacryl S1000 or Superose 6B. It permits recovery oI pla

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Amakasu, H., Y. Suzuki, M. Nishizawa, and T. Fukasawa. "Isolation and characterization of SGE1: a yeast gene that partially suppresses the gal11 mutation in multiple copies." Genetics 134, no. 3 (1993): 675–83. http://dx.doi.org/10.1093/genetics/134.3.675.

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Abstract Recessive mutations of GAL11 in Saccharomyces cerevisiae cause pleiotropic defects that include weak fermentation of galactose, alpha-specific sterility and slow growth on nonfermentable carbon sources. Recent experiments suggest that Gal11P functions as a "co-activator" that links transcriptional activators, such as Gal4p and Grf1p/Rap1p/Tuf1p, with the basic transcription machinery. In the present experiments we isolated a gene, SGE1, that suppresses gal11 for growth on ethidium bromide/galactose agar when the gene was present in two or more copies. The other gal11 phenotypes were n

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OPROMOLLA, Diltor Vladimir Araújo, Suzana MADEIRA, Andréa de Faria Fernandes BELONE, and Fátima Regina VILANI-MORENO. "Jorge Lobo’s disease: experimental inoculation in Swiss mice." Revista do Instituto de Medicina Tropical de São Paulo 41, no. 6 (1999): 359–64. http://dx.doi.org/10.1590/s0036-46651999000600005.

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Sixty-four isogenic Swiss mice were intradermically inoculated in both hind foot pads. The inocula, consisting of fungal suspensions from biopsies obtained from Jorge Lobo’s Disease patients, had the total number of fungi and the viability index determined using a Neubauer chamber and the fluorescein diacetate-ethidium bromide technique (FD-EB), respectively. The animals were sacrificed at times ranging from ten days to eighteen months after inoculation. The cellular infiltrate, mainly consisting of macrophages containing fungi, increased progressively up to end of the study; however, no macro

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14

Nihei, Oscar Kenji, Antonio Carlos Campos de Carvalho, Wilson Savino, and Luiz Anastacio Alves. "Pharmacologic properties of P2Z/P2X7receptor characterized in murine dendritic cells: role on the induction of apoptosis." Blood 96, no. 3 (2000): 996–1005. http://dx.doi.org/10.1182/blood.v96.3.996.

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Abstract In the immune system, extracellular adenosine 5′-triphosphate (ATP) mediates a variety of effects mainly through activation of a particular receptor subtype, the pore-forming P2Z/P2X7 purinoceptor. This purinergic receptor has been described chiefly in cells of hemopoietic origin such as T cells, thymocytes, monocytes, macrophages, and phagocytic cells of thymic reticulum. In this study, we characterized the P2Z/P2X7 purinoceptor and the ATP-mediated apoptosis in murine spleen–derived dendritic cells (DCs). Dye uptake and apoptosis were evaluated by flow cytometry. ATP-treated DCs wer

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Nihei, Oscar Kenji, Antonio Carlos Campos de Carvalho, Wilson Savino, and Luiz Anastacio Alves. "Pharmacologic properties of P2Z/P2X7receptor characterized in murine dendritic cells: role on the induction of apoptosis." Blood 96, no. 3 (2000): 996–1005. http://dx.doi.org/10.1182/blood.v96.3.996.015k02_996_1005.

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In the immune system, extracellular adenosine 5′-triphosphate (ATP) mediates a variety of effects mainly through activation of a particular receptor subtype, the pore-forming P2Z/P2X7 purinoceptor. This purinergic receptor has been described chiefly in cells of hemopoietic origin such as T cells, thymocytes, monocytes, macrophages, and phagocytic cells of thymic reticulum. In this study, we characterized the P2Z/P2X7 purinoceptor and the ATP-mediated apoptosis in murine spleen–derived dendritic cells (DCs). Dye uptake and apoptosis were evaluated by flow cytometry. ATP-treated DCs were permeab

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16

Grafström, Roland C., Charlotte C. Edman, Kristina Sundqvist, et al. "Cultured Human Bronchial Cells as a Model System in Lung Toxicology and Carcinogenesis: Implications from Studies with Acrolein." Alternatives to Laboratory Animals 16, no. 3 (1989): 231–43. http://dx.doi.org/10.1177/026119298901600305.

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Tissues and cells from both humans and laboratory animals can now be successfully cultured and used to examine in vitro the interspecial extrapolation of toxicity data. In particular, epithelial cells or fibroblasts from the human bronchus are systematically being used to study pathobiological effects of inhalation toxicants, e.g. acrolein, present in tobacco smoke and automobile emissions. Micromolar concentrations of acrolein are highly toxic to both bronchial epithelial cells and fibroblasts, and decrease their colony-forming efficiency, ability to exclude trypan blue dye, and content of lo

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Buroni, Silvia, Giulia Manina, Paola Guglierame, Maria Rosalia Pasca, Giovanna Riccardi, and Edda De Rossi. "LfrR Is a Repressor That Regulates Expression of the Efflux Pump LfrA in Mycobacterium smegmatis." Antimicrobial Agents and Chemotherapy 50, no. 12 (2006): 4044–52. http://dx.doi.org/10.1128/aac.00656-06.

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ABSTRACT The lfrA gene of Mycobacterium smegmatis encodes an efflux pump which mediates resistance to different fluoroquinolones, cationic dyes, and anthracyclines. The deletion of the lfrR gene, coding for a putative repressor and localized upstream of lfrA, increased the lfrA expression. In this study, reverse transcription-PCR experiments showed that the two genes are organized as an operon, and lacZ reporter fusions were used to identify the lfrRA promoter region. The lfrRA promoter assignment was verified by mapping the transcription start site by primer extension. Furthermore, we found t

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Martens-Habbena, Willm, and Henrik Sass. "Sensitive Determination of Microbial Growth by Nucleic Acid Staining in Aqueous Suspension." Applied and Environmental Microbiology 72, no. 1 (2006): 87–95. http://dx.doi.org/10.1128/aem.72.1.87-95.2006.

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ABSTRACT The determination of cell numbers or biomass in laboratory cultures or environmental samples is usually based on turbidity measurements, viable counts, biochemical determinations (e.g., protein and lipid measurements), microscopic counting, or recently, flow cytometric analysis. In the present study, we developed a novel procedure for the sensitive quantification of microbial cells in cultures and most-probable-number series. The assay combines fluorescent nucleic acid staining and subsequent fluorescence measurement in suspension. Six different fluorescent dyes (acridine orange, DAPI

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Maksum, Iman Permana, Suhaili Suhaili, Rizki Amalia, Dian Siti Kamara, Saadah Dian Rachman, and Rifky W. Rachman. "PCR Multipleks untuk Identifikasi Mycobacterium tuberculosis Resisten terhadap Isoniazid dan Rifampisin pada Galur Lokal Balai Laboratorium Kesehatan Provinsi Jawa Barat." Jurnal Kimia VALENSI 4, no. 2 (2018): 107–18. http://dx.doi.org/10.15408/jkv.v4i2.7226.

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The incidence of multidrug-resistant tuberculosis (MDR-TB) cases has become the biggest source of the problem in the effort to eradicate tuberculosis (TB) disease in Indonesia. MDR-TB is a resistant TB bacteria to the two, at least, first-line TB drugs, e.g., rifampin and isoniazid. Unfortunately, the current diagnostics methods to identify the MDR-TB are still slow, unspecific, and inaccurate. The purpose of this study is to identify the isoniazid- and rifampin-resistant M. tuberculosis (local strain Balai Laboratorium Kesehatan Provinsi Jawa Barat) by using multiplex PCR method. The TB bacte

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Bretagne, S., J. P. Guillou, M. Morand, and R. Houin. "Detection ofEchinococcus multilocularisDNA in fox faeces using DNA amplification." Parasitology 106, no. 2 (1993): 193–99. http://dx.doi.org/10.1017/s0031182000074990.

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SUMMARYIn order to identifyEchinococcus multilocularisDNA in fox faeces for epidemiological purposes, we have developed a new method to prepare DNA suitable for PCR amplification. DNA isolation from fox excrement was performed according to a novel procedure involving lysis in KOH, phenol–chloroform extraction and a purification step on a matrix (Prep-A-Gene®). The target sequence for amplification was theE. multilocularisU1 snRNA gene. PCR products were indistinguishable for 32 differentE. multilocularisisolates and no signal was observed after ethidium bromide staining with DNAs from other ta

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Tokunaga, Hiroko, Kenjiro Mitsuo, Sachiyo Ichinose, et al. "Salt-Inducible Multidrug Efflux Pump Protein in the Moderately Halophilic Bacterium Chromohalobacter sp." Applied and Environmental Microbiology 70, no. 8 (2004): 4424–31. http://dx.doi.org/10.1128/aem.70.8.4424-4431.2004.

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ABSTRACT It has been known that halophilic bacteria often show natural resistance to antibiotics, dyes, and toxic metal ions, but the mechanism and regulation of this resistance have remained unexplained. We have addressed this question by identifying the gene responsible for multidrug resistance. A spontaneous ofloxacin-resistant mutant derived from the moderately halophilic bacterium Chromohalobacter sp. strain 160 showed a two- to fourfold increased resistance to structurally diverse compounds, such as tetracycline, cefsulodin, chloramphenicol, and ethidium bromide (EtBr), and tolerance to

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James, R. Wright, Hua Yang, and Kent C. Dooley. "TILAPIA—A Source of Hypoxia-resistant Islet Cells for Encapsulation." Cell Transplantation 7, no. 3 (1998): 299–307. http://dx.doi.org/10.1177/096368979800700308.

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Encapsulation of pancreatic islets prevents graft revascularization after transplantation, resulting in graft hypoxia and attrition. Hypoxia-resistant islets would be ideal for encapsulation. Tilapia, a tropical teleost fish, have large, anatomically discrete islets that can be easily harvested without expensive, fickle islet isolation procedures and that provide mammalian-like glucose tolerance profiles when transplanted into diabetic recipients. Because tilapia can live in stagnant water, we speculated that tilapia islets might tolerate lower oxygen tensions than mammalian islets. Tilapia an

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Tirlapur, Uday K., Erhard Kranz, and Mauro Cresti. "Characterisation of isolated egg cells,in vitrofusion products and zygotes ofZea maysL. using the technique of image analysis and confocal laser scanning microscopy." Zygote 3, no. 1 (1995): 57–64. http://dx.doi.org/10.1017/s0967199400002380.

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SummaryChanges in membrane Ca2+, calcium receptor protein calmodulin, endoplasmic reticulum (ER), mitochondria and cellulose in unfixed, living, isolated egg cells and fusion products of pairs of one egg and one sperm cell ofZea maysL. have been investigated using chlorotetracycline, fluphenazine, immunocytochemical techniques, 3,3'dihexyloxa-carbocyanine iodide (DiOC6(3)) and calcofluor white in conjuction with computer-controlled video image analysis. In addition, confocal laser scannig microscopy has been used in conjuction with ethidium bromide to detect the nature and location of the sper

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Priya Vadhana, K. T., S. Parveen, B. Ushadevi, R. Selvakumar, S. Sangeetha, and S. Vairam. "New barium, strontium and strontium-doped barium squarates: synthesis, crystal structures and DNA/BSA binding, antioxidant and in vitro cytotoxicity studies." Acta Crystallographica Section C Structural Chemistry 75, no. 8 (2019): 1091–101. http://dx.doi.org/10.1107/s2053229619009082.

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A new set of differently hydrated barium and strontium squarates, namely poly[[triaqua(μ-1,2-dioxocyclobut-3-ene-1,2-diolato)barium] monohydrate], {[Ba(C4O4)(H2O)3]·H2O} n (1), poly[[diaqua(μ-1,2-dioxocyclobut-3-ene-1,2-diolato)strontium] monohydrate], {[Sr(C4O4)(H2O)2]·H2O} n (2), and poly[[triaqua(μ-1,2-dioxocyclobut-3-ene-1,2-diolato)barium/strontium(0.85/0.15)] monohydrate], {[Ba0.85Sr0.15(C4O4)(H2O)3]·H2O} n (3), is reported. The study of their crystal structures indicates that all the complexes crystallize in the triclinic space group P\overline{1}. Complexes 1 and 3 have a rare combinat

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Koshkaki, Elham Rahmanian, Abolghasem Abasi Sarcheshmeh, Vida Samiei, Farzad Rahmanian Koshkaki, Fatemeh Mohseni, and Mina Tajali. "Protective Effect of Aloe Vera Gel Extract on Histopathologic Changes in Ovaries and Gonadotropin in MS Model of Rat." Bangladesh Journal of Medical Science 19, no. 3 (2020): 444–52. http://dx.doi.org/10.3329/bjms.v19i3.45861.

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Objective: Multiple sclerosis (MS) is a self-immune demyelinating disorder of the CNS, followed by neuropathic discomfort, especially in young people in reproductive age. So, This research was conducted with the aim of assessing the protective effect of Aloe vera gel (AVG) extract on histopathologic changes of the ovary and gonadotropin in an experimental MS model of rat. Methods: In this research, 30 pieces of rats of Wistar race were randomly divided into 6 groups of 5. 20 pieces, 14μl of ethidium bromide was injected in their lateral ventricles to induce MS. In 5 pieces as control gro

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Brown, Stephen T., Jason L. Scragg, John P. Boyle, Kristin Hudasek, Chris Peers, and Ian M. Fearon. "Hypoxic Augmentation of Ca2+ Channel Currents Requires a Functional Electron Transport Chain." Journal of Biological Chemistry 280, no. 23 (2005): 21706–12. http://dx.doi.org/10.1074/jbc.m503144200.

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The incidence of Alzheimer disease is increased following ischemic episodes, and we previously demonstrated that following chronic hypoxia (CH), amyloid β (Aβ) peptide-mediated increases in voltage-gated L-type Ca2+ channel activity contribute to the Ca2+ dyshomeostasis seen in Alzheimer disease. Because in certain cell types mitochondria are responsible for detecting altered O2 levels we examined the role of mitochondrial oxidant production in the regulation of recombinant Ca2+ channel α1C subunits during CH and exposure to Aβ-(1–40). In wild-type (ρ+) HEK 293 cells expressing recombinant L-t

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Wisnoskey, Brian J., Mark Estacion, and William P. Schilling. "Maitotoxin-induced cell death cascade in bovine aortic endothelial cells: divalent cation specificity and selectivity." American Journal of Physiology-Cell Physiology 287, no. 2 (2004): C345—C356. http://dx.doi.org/10.1152/ajpcell.00473.2003.

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The maitotoxin (MTX)-induced cell death cascade in bovine aortic endothelial cells (BAECs), a model for Ca2+ overload-induced toxicity, reflects three sequential changes in plasmalemmal permeability. MTX initially activates Ca2+-permeable, nonselective cation channels (CaNSC) and causes a massive increase in cytosolic free Ca2+ concentration ([Ca2+]i). This is followed by the opening of large endogenous cytolytic/oncotic pores (COP) that allow molecules <800 Da to enter the cell. The cells then lyse not by rupture of the plasmalemma but through the activation of a “death” channel that lets

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Lee, H. Y., S. J. Yoon, K. A. Lee, and N. H. Kim. "239IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN PORCINE PARTHENOTES AT 2-CELL AND BLASTOCYST STAGES USING ANNEALING CONTROL PRIMER TECHNOLOGY." Reproduction, Fertility and Development 16, no. 2 (2004): 240. http://dx.doi.org/10.1071/rdv16n1ab239.

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Identification of embryo-specific genes will provide insight into early embryonic development. However, the current methods employed to identify genes expressed at specific stages are labor intensive and produce a high degree of false positives. In the present study we employed an accurate PCR technology controlled by an annealing control primer (ACP, SeeGene, Seoul, Korea) to identify differentially expressed genes in presumptive porcine parthenotes. In vitro-matured porcine oocytes were parthenogenetically activated by square electrical direct current pulses. After 3h of culture in North Car

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McClain, Jonathon L., and Brian D. Gulbransen. "The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins." Journal of Neurophysiology 117, no. 1 (2017): 365–75. http://dx.doi.org/10.1152/jn.00507.2016.

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Glia play key roles in the regulation of neurotransmission in the nervous system. Fluoroacetate (FA) is a metabolic poison widely used to study glial functions by disrupting the tricarboxylic acid cycle enzyme aconitase. Despite the widespread use of FA, the effects of FA on essential glial functions such as calcium (Ca2+) signaling and hemichannel function remain unknown. Therefore, our goal was to assess specifically the impact of FA on essential glial cell functions that are involved with neurotransmission in the enteric nervous system. To this end, we generated a new optogenetic mouse mode

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Hong, DS, C. Beckham, R. Huss, et al. "Major histocompatibility complex class II-mediated inhibition of hematopoiesis in long-term marrow cultures involves apoptosis and is prevented by c-kit ligand [see comments]." Blood 86, no. 9 (1995): 3341–52. http://dx.doi.org/10.1182/blood.v86.9.3341.bloodjournal8693341.

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Expression of major histocompatibility complex (MHC) class II molecules is developmentally regulated and lineage dependent. Their role in hematopoiesis is not well defined. Previous studies in a canine model showed that dogs given 920 cGy of total body irradiation, transplanted with autologous marrow, and treated with anti-MHC class II monoclonal antibody (MoAb) immediately posttransplant experienced only a transient granulocyte recovery that was followed by graft failure. In the present study, the effect of anti-MHC class II MoAbs on canine in vitro hematopoiesis was investigated. Anti-MHC cl

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Prütz, W. A. "Interaction between glutathione and Cu(II) in the vicinity of nucleic acids." Biochemical Journal 302, no. 2 (1994): 373–82. http://dx.doi.org/10.1042/bj3020373.

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GSH interacts with Cu(II) in the vicinity of DNA (pH approximately 7) to form the DNA-Cu(I) complex, which can be quantified by characteristic absorption changes [e.g. delta epsilon 295 = 4516 cm-1.M-1 Cu(I)]. Under initial conditions of Cu(II)/GSH >> 1 and DNA(base)/Cu(II) >> 5, the stoichiometry is 1 DNA-Cu(I) per SH group (also for other thiols). Stopped-flow kinetics show that the complex is formed with half-lives of 1-30 s, depending on the environment, but independent of O2. DNA-Cu(I) generation is much slower, less efficient, and O2-dependent at Cu(II)/GSH < 1, or when GS

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Baji Gal, A., J. W. Carnwath, A. Dinnyes, D. Herrmann, C. Wrenzycki, and H. Niemann. "210 COMPARISON OF REAL-TIME PCR AND END-POINT PCR FOR ANALYSIS OF GENE EXPRESSION IN PREIMPLANTATION EMBRYOS." Reproduction, Fertility and Development 17, no. 2 (2005): 255. http://dx.doi.org/10.1071/rdv17n2ab210.

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The purpose of this study was to compare real-time PCR and end-point PCR with respect to their suitability for the analysis of gene expression in samples in which the number of cells is limited, for example, in studies of pre-implantation embryonic development. The real-time instrument was a LightCycler® from Roche Diagnostics (Budaors, Hungary) which is a capillary-based PCR system. Primers for histone H2A (housekeeping gene) were used for all PCR reactions. The end-point PCR system included an MJ Research PT-100 thermocycler, agarose gel electrophoresis, ethidium bromide staining, and image

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Lloyd, R. E., R. Alberio, E. J. Bowles, K. H. S. Campbell, and J. C. St. John. "54THE DEVELOPMENT AND CHARACTERIZATION OF MITOCHONDRIAL DNA (MTDNA)-DEPLETED CAPRA HIRCUS FETAL FIBROBLASTS: CANDIDATE DONORS FOR SOMATIC CELL NUCLEAR TRANSFER (SCNT)." Reproduction, Fertility and Development 16, no. 2 (2004): 149. http://dx.doi.org/10.1071/rdv16n1ab54.

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Mammalian mtDNA is approximately 16.6kb in size. It has 37 genes, 13 of which encode protein subunits of the oxidative phosphorylation (OXPHOS) system, the major ATP-generating pathway of the cell. Normally, mammals inherit a single mtDNA genome (homoplasmy) from their mother. Somatic cell nuclear transfer (SCNT) violates this strict maternal, homoplasmic inheritance of mtDNA as cytoplasm is transferred along with the nucleus, which often results in an oocyte harboring both donor and recipient mtDNA genomes (heteroplasmy). This been previously reported (reviewed St. John JC 2002 Theriogenology

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Basso, M., D. Spada, A. Modoni, et al. "SK3 polyglutamine tract polymorphism and acute oxaliplatin neurotoxicity: A translational study." Journal of Clinical Oncology 25, no. 18_suppl (2007): 21076. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.21076.

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21076 Background: Acute neurotoxicity of l-oxaliplatin (l-OHP), characterized by cold-induced paresthesias, muscle cramps and tightness, seems electrophysiologically related to an abnormal nerve hyperexcitability (NHE) resembling neuromyotonia, an autoimmune disease caused by autoantibodies directed against voltage-gated potassium channels (VGKC). This l-OHP-related channelopathy seems to be reversible, but there is no agreement about the channel involved, most investigators favouring voltage-gated sodium channels. SK3 VGKC are mainly located in the peripheral nervous system and are characteri

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Romashchenko, O. V. "Personalized trimetazidine prescription as a cytoprotective agent in patients with coronary artery disease." Russian Journal of Cardiology 26, no. 6 (2021): 4532. http://dx.doi.org/10.15829/1560-4071-2021-4532.

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Aim. To develop a personalized approach to the trimetazidine use in patients with coronary artery disease (CAD) based on the criteria for predicting the cytoprotective activity tested in vitro.Material and methods. We examined 30 patients with class I-III stable effort angina with concomitant hypertension and heart failure. The patients underwent echocardiography, complete blood count, biochemical tests with determination of the lipid profile, creatine phosphokinase (CPK), CPK-MB, renal and hepatic parameters. To determine the cytoprotective activity of trimetazidine, white blood cells (WBCs)

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O’Callaghan, Yvonne C., Julie A. Woods та Nora M. O’Brien. "Limitations of the single-cell gel electrophoresis assay to monitor apoptosis in U937 and HepG2 cells exposed to 7β-hydroxycholesterol11Abbreviations: TUNEL, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labelling of fragmented nuclear DNA in situ; 7βOHC, 7β-hydroxycholesterol; EtBr, Ethidium bromide." Biochemical Pharmacology 61, № 10 (2001): 1217–26. http://dx.doi.org/10.1016/s0006-2952(01)00587-1.

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Cruz Salazar, Bárbara, Consuelo Lorenzo, Eduardo Espinoza Medinilla, and Sergio López. "Variation and genetic structure of the endangered Lepus flavigularis (Lagomorpha: Leporidae)." Revista de Biología Tropical 65, no. 4 (2017): 1322. http://dx.doi.org/10.15517/rbt.v65i4.27882.

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Lepus flavigularis, is an endemic and endangered species, with only four populations inhabiting Oaxaca, México: Montecillo Santa Cruz, Aguachil, San Francisco del Mar Viejo and Santa María del Mar. Nevertheless, human activities like poaching and land use changes, and the low genetic diversity detected with mitochondrial DNA and allozymes in previous studies, have supported the urgent need of management strategies for this species, and suggest the definition of management units. For this, it is necessary to study the genetic structure with nuclear genes, due to their inheritance and high polym

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Bakhtou, Hossein, Asiie Olfatbakhsh, Abdolkhaegh Deezagi, and Ghasem Ahangari. "The Expression of Dopamine Receptors Gene and their Potential Role in Targeting Breast Cancer Cells with Selective Agonist and Antagonist Drugs. Could it be the Novel Insight to Therapy?" Current Drug Discovery Technologies 16, no. 2 (2019): 184–97. http://dx.doi.org/10.2174/1570163815666180130101421.

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Background:Breast cancer is one of the common causes of mortality for women in Iran and other parts of the world. The substantial increasing rate of breast cancer in both developed and developing countries warns the scientists to provide more preventive steps and therapeutic measures. This study is conducted to investigate the impact of neurotransmitters (e.g., Dopamine) through their receptors and the importance of cancers via damaging immune system. It also evaluates dopamine receptors gene expression in the women with breast cancer at stages II or III and dopamine receptor D2 (DRD2) related

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Zhao, Ran, Sarah Zarabi, Takenobu Nii, et al. "Dual Targeting of Mitochondrial Unfolded Protein Response and BCL2 in Acute Myeloid Leukemia." Blood 134, Supplement_1 (2019): 2562. http://dx.doi.org/10.1182/blood-2019-127101.

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Cellular stress response has dual aspects; cell-protective or lethal. Mitochondria have their unique organellar response termed "mitochondrial unfolded protein response (UPRmt)" induced by damaged mitochondrial (mt) matrix proteins. While recent discoveries have successfully targeted BCL2, a regulator of mt integrity in acute myeloid leukemia (AML), the significance of UPRmt is unknown. We hypothesized that priming UPRmt towards cell death would be a novel therapeutic strategy for AML. UPRmt is generally induced by dysregulation of mt protein pools. Therefore, to test if UPRmt signaling is als

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Pedersen, HG, ED Watson, and EE Telfer. "Analysis of atresia in equine follicles using histology, fresh granulosa cell morphology and detection of DNA fragmentation." Reproduction, March 1, 2003, 417–23. http://dx.doi.org/10.1530/rep.0.1250417.

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Follicular atresia has been examined previously by various biochemical and histological methods. The aim of this study was to compare, for the first time, detection of granulosa cell apoptosis by biochemical DNA analysis and microscopic examination of fresh granulosa cell morphology with the established method of detecting atresia by histology in equine follicles. DNA extracted from granulosa cells was examined by staining with ethidium bromide and end-labelling with [(32)P]dideoxy-ATP, which labels the free 3'-end of DNA fragments. In 25 of 26 follicles (96%) there was agreement between end-l

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Kapoor, Tarun, and Sidharth Mehan. "Neuroprotective methodologies in treatment of Multiple Sclerosis: Current status of Clinical and Pre-clinical findings." Current Drug Discovery Technologies 17 (February 7, 2020). http://dx.doi.org/10.2174/1570163817666200207100903.

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: Multiple sclerosis an idiopathic and autoimmune associated motor neuron disorder that attacks myelinated neurons in specific brain regions of young people, especially females; characterized by oligodendrocytes destruction causes demyelination, neuroinflammation, mitochondrial abnormalities, oxidative stress and neurotransmitter deficits associated with motor and cognitive dysfunctions, vertigo and muscle weakness. The limited intervention of pharmacologically active compounds like interferon-β, mitoxantrone, fingolimod and monoclonal antibodies used clinically are majorly associated with adv

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Ayoade, Femi,, Scott Fayemi, Tosin Ogunbiyi, Olumide Olukanni, and Paul Oluniyi. "Disparity in Phenotypic and Genetic Characterization of Lactic Acid Microorganisms Isolated from Spontaneous Fermentation of Yam (Dioscorea rotundata)." Asian Food Science Journal, April 9, 2019, 1–7. http://dx.doi.org/10.9734/afsj/2019/v8i229988.

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Aim: In order to ensure the accurate taxonomic identification of the lactic acid organisms that were previously isolated from spontaneously fermented yam for safety assessment and quality assurance purposes, phenotypic and genetic identification data were compared. Study Design: Using the purposive sampling method, four microorganisms were characterized using molecular methods. Place and Duration of Study: Isolates of lactic acid microorganisms (2 bacterial and 2 fungal organisms) from spontaneously fermented yam in a previous study carried out in May 2016 were genetically identifi

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George, Ajith J., Bibu J. Kariyil, Usha P. T. Ayyappan, and Anu Gopalakrishnan. "Chloroform Fraction of Methanolic Extract of Seeds of Annona muricata Induce S Phase Arrest and ROS Dependent Caspase Activated Mitochondria Mediated Apoptosis in Triple Negative Breast Cancer." Anti-Cancer Agents in Medicinal Chemistry 20 (September 18, 2020). http://dx.doi.org/10.2174/1871520620666200918101448.

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Background: Triple negative breast cancers (TNBCs) are having high morbidity and shorter survival rate in the population. These types of cancers are having high aggressiveness, lymphatic invasion and absence of receptors. The treatment options for these types of cancers are also scarce. Several studies have been conducted to investigate the effectiveness of seeds of Annona muricata for its anti cancer activities in various cancer cell lines such as lung A549, breast MCF7, colon HT-29, oral KB and human hepatoma cell lines. But works related to its anticancer effect and mechanism of action in T

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