Relevant bibliographies by topics / FCS-free culture

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Academic literature on the topic 'FCS-free culture'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "FCS-free culture"

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Looney, Ben, and ChangQing Xia. "Murine bone marrow-derived dendritic cells generated in serum free conditions display an immature phenotype and protect from T1D. (107.11)." Journal of Immunology 186, no. 1_Supplement (2011): 107.11. http://dx.doi.org/10.4049/jimmunol.186.supp.107.11.

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Abstract Dendritic cells possess the ability to modulate immune responses and therefore have been investigated as a cell based therapy to prevent or reverse Type 1 Diabetes. In the NOD mouse model, bone marrow-derived DCs (BM-DC) are generated from bone marrow precursors cultured with GM-CSF and IL-4 and also typically containing fetal calf serum (FCS). The effect of FCS on murine BM-DC has not been extensively investigated. In the present study we compare BM-DC generated in FCS free culture media (X-VIVO20) with BM-DC generated in culture media (RPMI1640) containing 10% FCS. Data show that CD
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Kočiková, Alena, Andrea Kolesarić, Frieder Koszik, Georg Stingl, and Adelheid Elbe-Bürger. "Murine Langerhans Cells Cultured Under Serum-Free Conditions Mature into Potent Stimulators of Primary Immune Responses In Vitro and In Vivo." Journal of Immunology 161, no. 8 (1998): 4033–41. http://dx.doi.org/10.4049/jimmunol.161.8.4033.

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Abstract The ability of Ag-pulsed dendritic cells (DC) to induce primary immune responses has led them to be used for vaccination purposes. However, irrelevant Ags (e.g., FCS) can also be taken up by DC during their isolation and culture and then presented in vivo. To circumvent this, we have established a serum-free (SF) culture system. Murine epidermal cell (EC) suspensions were prepared with and without FCS and cultured for 3 days either in SF or FCS-containing medium. In spite of the lower Langerhans cell (LC) yields under SF conditions, both SF- and FCS-cultured LC (SF-cLC, FCS-cLC) under
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Fujimori, Y., M. Ogawa, SC Clark, and GJ Dover. "Serum-free culture of enriched hematopoietic progenitors reflects physiologic levels of fetal hemoglobin biosynthesis." Blood 75, no. 8 (1990): 1718–22. http://dx.doi.org/10.1182/blood.v75.8.1718.1718.

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Abstract Adult erythroid progenitors produce significantly higher fetal hemoglobin (HbF) levels in cultures containing fetal calf serum (FCS) and erythropoietin (Ep) than in vivo. The precise mechanisms for this increased HbF production in culture have not been elucidated. We examined HbF biosynthesis by enriched human progenitors in serum-free (SF) culture. We measured globin chain biosynthesis by combination of isoelectric focusing and autoradiography and examined percent nucleated erythrocytes containing HbF (%FNRBC) using microscopic immunodiffusion. CD34 (My10)-positive marrow cells from
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Fujimori, Y., M. Ogawa, SC Clark, and GJ Dover. "Serum-free culture of enriched hematopoietic progenitors reflects physiologic levels of fetal hemoglobin biosynthesis." Blood 75, no. 8 (1990): 1718–22. http://dx.doi.org/10.1182/blood.v75.8.1718.bloodjournal7581718.

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Adult erythroid progenitors produce significantly higher fetal hemoglobin (HbF) levels in cultures containing fetal calf serum (FCS) and erythropoietin (Ep) than in vivo. The precise mechanisms for this increased HbF production in culture have not been elucidated. We examined HbF biosynthesis by enriched human progenitors in serum-free (SF) culture. We measured globin chain biosynthesis by combination of isoelectric focusing and autoradiography and examined percent nucleated erythrocytes containing HbF (%FNRBC) using microscopic immunodiffusion. CD34 (My10)-positive marrow cells from a normal
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Yeo, C. X., J. Rathjen, and D. K. Gardner. "112. FETAL CALF SERUM AFFECTS hESC METABOLISM AND GENE EXPRESSION LEADING TO DIFFERENTIATION IN CULTURE." Reproduction, Fertility and Development 22, no. 9 (2010): 30. http://dx.doi.org/10.1071/srb10abs112.

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Fetal calf serum (FCS) has conventionally been used to support the growth and maintenance of human embryonic stem cells (hESCs). FCS however, is an undefined complex mixture containing factors which potentially alter the functionality of hESCs. Inclusion of FCS during embryo culture negatively impacts embryo metabolism and viability but comparative studies on hESCs have been hindered by the lack of serum and feeder independent culture systems. Using a recently available defined culture system, the effects of FCS on hESC metabolism and pluripotentcy were investigated. Mel2 hESCs were grown at 3
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Daniaux, C., B. Verhaeghe, and I. Donnay. "133 INSULIN, TRANSFERRIN AND SELENIUM WITH OR WITHOUT BSA IN A SERUM-FREE CULTURE SYSTEM FOR BOVINE EMBRYO, AND ITS SUITABILITY FOR EMBRYOS CULTURED IN SMALL GROUPS." Reproduction, Fertility and Development 17, no. 2 (2005): 217. http://dx.doi.org/10.1071/rdv17n2ab133.

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Serum in embryo culture medium may be a potential cause of abnormal accumulation of lipid droplets, which is correlated to a higher sensitivity to cryopreservation. Moreover, serum may introduce pathogens. With the aim of developing a serum-free culture medium, we first (Experiment 1) investigated the effect of adding ITS (5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium) as a serum substitute in SOF medium on embryos cultured in large groups (20 embryos per culture drop of 20 μL) and we then (Experiment 2) analyzed the effect of adding BSA. In this second experiment, our serum-free cult
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Suzuki, Takao, Masatomo Takahashi, Tsukasa Miyagi, et al. "A Study on the Induction of Mesoderm Differentiation of Mouse Embryonic Stem Cell in Serum Free Culture." Blood 108, no. 11 (2006): 4161. http://dx.doi.org/10.1182/blood.v108.11.4161.4161.

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Abstract (Introduction) Several products which can maintain the growth of ES (Embryonic stem) cell with no feeder cells or fetal calf serum (FCS) is now commercially available. Culti Cell ®(CC) can be a substitute for FCS and has ability of mainitaining mouse ES cells without feeder cells. Previously, it was reported that ES cell differentiate into FLk (fetal liver kinase) positive cells derived from mesoderm in methylcellulose and FCS. We investigated whether ES cell is able to differentiate into Flk positive cell in CC and methylcellulose without FCS. (Materials and Methods) Thawed ES cells
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Wanner, Yvonne, Felix Umrath, Marc Waidmann, Siegmar Reinert, and Dorothea Alexander. "Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization." Stem Cells International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/8303959.

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Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we detected significantly higher population doubling times of PL-cultured JPCs in comparison to FCS-cultured JPCs. However, this result was not based on lower proliferation activities but on diminished cell si
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Sébire, G., D. Emilie, C. Wallon, et al. "In vitro production of IL-6, IL-1 beta, and tumor necrosis factor-alpha by human embryonic microglial and neural cells." Journal of Immunology 150, no. 4 (1993): 1517–23. http://dx.doi.org/10.4049/jimmunol.150.4.1517.

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Abstract The abilities of the different cells from human central nervous system (CNS) to produce IL-6, IL-1 beta, and TNF-alpha were tested in vitro using either cultures enriched in human embryonic microglial cells or primary cultures of human embryonic CNS cells. High amounts of IL-6, low amounts of IL-1 beta but no TNF-alpha were detected in supernatants of microglial cells, kept either in FCS-free conditions or in FCS-containing medium. Moreover, IL-6 mRNA was also present in 45 to 55% of microglial cells cultured in the presence of FCS as visualized by in situ hybridization, whereas IL-1
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Pöhland, R., S. Lenz, J. Vanselow, and W. Tomek. "Detection of gene expression and enzyme activity of Cytochrom P450 arom (aromatase) in preantral and early antral bovine follicles depending on culture conditions in vitro." Archives Animal Breeding 49, no. 1 (2006): 29–40. http://dx.doi.org/10.5194/aab-49-29-2006.

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Abstract. This study reveals that cultivation of preantral and early antral (< 500 μm) follicles in culture medium containing FCS results in an expression of cytochrome P450 arom. (aromatase). The enzymatic activity of aromatase, measured in terms of the estradiol synthesis, was proved to be present in follicles greater than 100 μm, could further be stimulated by FSH in follicles greater than 300 μm diameter. The enzyme protein and the gene expression were studied by means of western blot and immunohistochemistry as well as by means of realtime PCR. Neither the protein nor the corresponding
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Book chapters on the topic "FCS-free culture"

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Suzuki, Keiiciiiro. "Cell culture model for oxidative stress." In Experimental protocols for reactive oxygen and nitrogen species. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780198506683.003.0081.

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Abstract Cells in culture are widely used as experimental models to study pro-oxidant and antioxidant responses. Although the conditions used are usually simple, and the results are obtained easily and rapidly, proteins, e.g. foetal calf serum, can act as radical scavengers, and iron and copper salts can also contaminate culture media during free-radical experiments. Appropriate controls must therefore be included and conditions carefully standardized, for example by using foetal calf serum (FCS)-free media.
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Conference papers on the topic "FCS-free culture"

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NAKAMURA, Shin. "MONOCYTE/MACROPHAGE TISSUE FACTOR: ROLE OF ITS N-GLYCOSYLATED CARBOHYDRATE MOIETY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643286.

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Monocytes/macrophages and related cells are known to generate tissue factor (TF) , a membrane associated lipid-glycoprotein complex, following activation with LPS or other stimuli. Monkey (M. fuscata) mononuclear leukocytes (MNL, 3 × 106/ml) cultured with LPS (lµg/ml) in FCS-free RPMI medium were stimulated to produce the glycoprotein (TF-Apo). After a lag period of 2 h the TF-Apo production was initiated, and its accumulation reached the plateau after 12 h and then declined to approximately half of the maximum level after 24 h. A time course of the TF activity was strictly in accord with that
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