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Journal articles on the topic 'Fetal Fibroblast'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Fabisiak, J. P., M. Absher, J. N. Evans, and J. Kelley. "Spontaneous production of PDGF A-chain homodimer by rat lung fibroblasts in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 263, no. 2 (1992): L185—L193. http://dx.doi.org/10.1152/ajplung.1992.263.2.l185.

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Platelet-derived growth factor (PDGF) is considered a decisive mediator of fibroblast growth and phenotype within the lung. The cellular sources of PDGF within the lung remain undefined. The ability of lung fibroblasts themselves to produce PDGF in vitro was therefore investigated. Northern and Western blot analyses revealed the expression of PDGF-A mRNA and secretion of A-chain containing proteins by fibroblasts derived from adult and fetal rat lung. PDGF-A gene or protein expression were below the limits of detection in two human lung fibroblast lines examined in a similar manner. PDGF-B tra

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2

Navarro, Angels, Mo Rezaiekhaligh, J. Andrew Keightley, Sherry M. Mabry, Ricardo E. Perez, and Ikechukwu I. Ekekezie. "Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts." American Journal of Physiology-Lung Cellular and Molecular Physiology 296, no. 6 (2009): L928—L935. http://dx.doi.org/10.1152/ajplung.00012.2009.

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Acute lung injury involving extremely immature lungs often heals without excessive fibrosis unlike later in gestation and in adults. Several factors may be involved, but fibroblast contraction of collagen has been linked to the level of wound fibrosis. To assess whether human lung fibroblasts of fetal versus adult origin differ in ability to contract collagen and define the molecular underpinnings, we performed three-dimensional collagen contraction assay, analyzed their differential mRNA profile, specifically for transforming growth factor-β (TGF-β) signaling pathway and extracellular matrix

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3

Irwin, C. R., M. Picardo, I. Ellis, et al. "Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing." Journal of Cell Science 107, no. 5 (1994): 1333–46. http://dx.doi.org/10.1242/jcs.107.5.1333.

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We have previously reported that fetal and adult skin fibroblasts display distinctive migratory phenotypes on 3-D collagen substrata and that these behavioural characteristics may be quantified by a function defined as the cell density migration index (CDMI). Subsequent work indicated that this difference in migratory phenotype was due to the production by fetal fibroblasts of a migration stimulating factor (MSF) that is not produced by normal adult skin fibroblasts. We now present data indicating that: (a) unselected fibroblasts obtained from 14/14 (100%) of adult gingival explants expressed

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4

Rettig, W. J., H. P. Erickson, A. P. Albino, and P. Garin-Chesa. "Induction of human tenascin (neuronectin) by growth factors and cytokines: cell type-specific signals and signalling pathways." Journal of Cell Science 107, no. 2 (1994): 487–97. http://dx.doi.org/10.1242/jcs.107.2.487.

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The extracellular matrix protein tenascin (TN) is expressed with precise temporo-spatial patterns during embryonic and fetal development and is induced in healing wounds, inflammatory lesions and solid tumors. These tissue patterns suggest that TN synthesis may be modulated by soluble factors present in developing tissues or released from injured, inflammatory or neoplastic cells. To characterize the extrinsic control of human TN we examined the effects of several signalling molecules on cultured neural, melanocytic and fibroblastic cells. Results obtained with alpha TN antibodies in enzyme-li

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5

Lorenz, H. Peter, Richard Y. Lin, Michael T. Longaker, David J. Whitby, and N. Scott Adzick. "The Fetal Fibroblast." Plastic and Reconstructive Surgery 96, no. 6 (1995): 1251–59. http://dx.doi.org/10.1097/00006534-199511000-00001.

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6

Das, M., K. R. Stenmark, and E. C. Dempsey. "Enhanced growth of fetal and neonatal pulmonary artery adventitial fibroblasts is dependent on protein kinase C." American Journal of Physiology-Lung Cellular and Molecular Physiology 269, no. 5 (1995): L660—L667. http://dx.doi.org/10.1152/ajplung.1995.269.5.l660.

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The earliest and most striking proliferative changes in the neonatal pulmonary arterial wall occur in the adventitia where the fibroblast resides. The protein kinase C (PKC) pathway is developmentally regulated and important in vascular cell growth. We tested the hypothesis that developmental differences in growth of pulmonary artery adventitial fibroblasts would be detectable in vitro and dependent on PKC. Fibroblasts were isolated from bovine fetal, neonatal, and adult pulmonary arteries. Growth was measured by [3H]thymidine incorporation and cell counts. Under serum-stimulated conditions, f

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7

Caniggia, I., I. Tseu, R. N. Han, B. T. Smith, K. Tanswell, and M. Post. "Spatial and temporal differences in fibroblast behavior in fetal rat lung." American Journal of Physiology-Lung Cellular and Molecular Physiology 261, no. 6 (1991): L424—L433. http://dx.doi.org/10.1152/ajplung.1991.261.6.l424.

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Fibroblast-epithelial interactions were investigated in cells from late-gestation fetal rat lung. Fibroblasts from the pseudoglandular stage of lung development stimulated epithelial cell proliferation, whereas fibroblasts from the saccular stage promoted epithelial cell differentiation. The developmental switch from proliferation to differentiation seemed to be controlled by both cell types. Fibroblast-derived epithelial cell growth-promoting activity, evident in cells from the pseudoglandular period, decreased during development and almost disappeared in cells from the saccular stage. Intere

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8

Ellis, I., J. Banyard, and S. L. Schor. "Differential response of fetal and adult fibroblasts to cytokines: cell migration and hyaluronan synthesis." Development 124, no. 8 (1997): 1593–600. http://dx.doi.org/10.1242/dev.124.8.1593.

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Previous studies have indicated that fetal skin fibroblasts display an elevated level of migratory activity compared to adult cells and that this may result from inherent differences in the production of hyaluronan (HA) by these cells. Data presented in this communication indicate that the elevated level of fetal fibroblast migration into 3D-collagen gels and HA synthesis by these cells were not affected by epidermal growth factor (EGF), platelet-derived growth factor (PDGF), acidic fibroblast growth factor (aFGF) or basic fibroblast growth factor (bFGF). In contrast, both cell migration and H

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9

Chen, Xi, Nathalie Hirt-Burri, Corinne Scaletta, Alexis E. Laurent, and Lee Ann Applegate. "Bicomponent Cutaneous Cell Therapy for Early Burn Care: Manufacturing Homogeneity and Epidermis-Structuring Functions of Clinical Grade FE002-SK2 Allogeneic Dermal Progenitor Fibroblasts." Pharmaceutics 17, no. 6 (2025): 692. https://doi.org/10.3390/pharmaceutics17060692.

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Background: The extracellular matrix (ECM), primarily composed of collagen and elastin synthesized by dermal fibroblasts, is critical for mesenchymal tissue integrity. Fibroblast phenotypes vary significantly with the anatomical location and developmental stage. Fetal skin, particularly prior to 14 weeks of gestation, exhibits a simplified structure compared to adult skin, characterized by a thin, loose dermal matrix and a single-layered epithelium. Objectives: This study aimed to characterize and functionally compare homogenous progenitor fetal fibroblast (PFF) populations derived from 14-wee

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10

Kelley, J., J. P. Fabisiak, K. Hawes, and M. Absher. "Cytokine signaling in lung: transforming growth factor-beta secretion by lung fibroblasts." American Journal of Physiology-Lung Cellular and Molecular Physiology 260, no. 2 (1991): L123—L128. http://dx.doi.org/10.1152/ajplung.1991.260.2.l123.

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Control of growth and phenotypic expression of interstitial fibroblasts is a critical determinant of lung architecture and physiology during processes of growth and remodeling. We examined the ability of lung fibroblasts to produce transforming growth factor-beta (TGF-beta), a cytokine that is known to modulate proliferation and phenotypic expression of mesenchymal cells. Cultures of fibroblasts isolated from rat lungs spontaneously secrete TGF-beta as measured in the standard bioassay of anchorage-independent growth of normal rat kidney (NRK) cells in soft agar. Rat lung fibroblasts secrete T

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11

Ferretti, Emanuela, Song Li, Jinxia Wang, Martin Post, and Aideen Moore. "Mesenchymal Regulation of Differentiation of Intestinal Epithelial Cells." Journal of Pediatric Gastroenterology and Nutrition 23, no. 1 (1996): 65–73. http://dx.doi.org/10.1002/j.1536-4801.1996.tb00303.x.

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SummaryIntestinal epithelial differentiation requires the presence of mesenchyme, but the exact nature of this epithelial‐mesenchymal interaction is unknown. The role of humoral and extracellular matrix factors in determining intestinal epithelial differentiation was examined by exposing a nontransformed, immature, rat small‐intestinal cell line (IEC‐6) to intestinal fibroblast‐conditioned media or by growing the cells on matrix deposited by intestinal fibroblasts. Differentiation was assessed morphologically and by induction of the disaccharidase sucrase, which is a marker of intestinal epith

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Chambers, R. C., R. J. McAnulty, A. Shock, J. S. Campa, A. J. Newman Taylor, and G. J. Laurent. "Cadmium selectively inhibits fibroblast procollagen production and proliferation." American Journal of Physiology-Lung Cellular and Molecular Physiology 267, no. 3 (1994): L300—L308. http://dx.doi.org/10.1152/ajplung.1994.267.3.l300.

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Chronic inhalation of cadmium fumes has been associated with the development of emphysema, a disease characterized by extensive disruption of lung connective tissue. Cadmium is also an important contaminant of tobacco and may play a role in cigarette smoking-related emphysema. In this paper we investigated the effect of nontoxic doses of cadmium chloride (CdCl2) on fibroblast procollagen production and proliferation, key features of connective tissue repair following injury. CdCl2 inhibited fibroblast procollagen production in a dose-dependent manner in two different cell lines. For fetal rat

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13

Sandulache, Vlad C., Joseph E. Dohar, and Patricia A. Hebda. "Adult-Fetal Fibroblast Interactions: Effects on Cell Migration and Implications for Cell Transplantation." Cell Transplantation 14, no. 5 (2005): 331–37. http://dx.doi.org/10.3727/000000005783983025.

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Wound healing is a complex process involving close cooperation between multiple cell types. During wound healing, fibroblasts are primarily responsible for synthesis of the replacement extracellular matrix. Fibroblast therapy is under investigation in this and other laboratories for its potential use to modulate the final outcome of the wound-healing process. This study addresses the potential interactions between transplanted and host fibroblasts, using a two-dimensional mixed culture model. Our results show that fibroblasts of two different phenotypes, fetal and adult, exhibit different spee

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14

Sakai, Shigeki, Noriko Aramaki-Hattori, and Kazuo Kishi. "Fetal Fibroblast Transplantation via Ablative Fractional Laser Irradiation Reduces Scarring." Biomedicines 11, no. 2 (2023): 347. http://dx.doi.org/10.3390/biomedicines11020347.

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Scar treatments include fractional laser treatment, cell transplantation, surgery, skin needling, and dermal fillers. Fractional laser treatments are used to reduce scarring and blurring. Cell transplantation is promising, with mature fibroblasts and adipose-derived stem cells being used clinically, while embryonic fibroblasts are used experimentally. Herein, we developed a combination of ablative CO2 (carbon dioxide) fractional laser and cell transplantation for the treatment of scars. Eight-week-old male C57Bl/6 mice were used to create a full-layer skin defect in the back skin and create sc

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15

Smith, B. T., and M. Post. "Fibroblast-pneumonocyte factor." American Journal of Physiology-Lung Cellular and Molecular Physiology 257, no. 4 (1989): L174—L178. http://dx.doi.org/10.1152/ajplung.1989.257.4.l174.

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Fibroblast-pneumonocyte factor (FPF) is an organ-specific differentiation factor produced, under glucocorticoid regulation, by the fetal lung fibroblast, which in turn enhances the differentiation of the alveolar type II cell with respect to surfactant production. In this article we review the appearance of the type II cell phenotype during fetal life and the action of FPF to time full acquisition of this phenotype. Evidence supporting a physiological role for this factor is reviewed as are details of its production by the fetal lung fibroblast and action on the type II cell. Finally we summar

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16

Reiss, K., W. Cheng, J. Kajstura, E. H. Sonnenblick, L. G. Meggs, and P. Anversa. "Fibroblast proliferation during myocardial development in rats is regulated by IGF-1 receptors." American Journal of Physiology-Heart and Circulatory Physiology 269, no. 3 (1995): H943—H951. http://dx.doi.org/10.1152/ajpheart.1995.269.3.h943.

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To determine whether the growth of cardiac fibroblasts during development is modulated by the insulin-like growth factor (IGF)-1 receptor (IGF-1R), the expression of IGF-1, IGF-2, and IGF-1R was determined in fibroblasts from fetal and postnatal hearts. The expression of proliferating cell nuclear antigen (PCNA) and DNA polymerase-alpha was also evaluated in combination with the estimation of DNA replication. In comparison with fetal hearts, at postnatal day 21, fibroblast expression of IGF-1R mRNA, IGF-2, PCNA, and DNA polymerase-alpha was reduced by 77, 70, 80, and 86%, respectively. Moreove

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17

Zawahir, Shamila, Adora Okogbule-Wonodi, Guanghui Li, and Thomas Blanchard. "The role of fibroblasts in neonatal intestinal inflammation (89.46)." Journal of Immunology 184, no. 1_Supplement (2010): 89.46. http://dx.doi.org/10.4049/jimmunol.184.supp.89.46.

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Abstract Background: Fetal intestinal fibroblast cell lines (FIF) produce more IL-8 in response to LPS compared to non-neonatal intestinal fibroblast lines (NNIF). Necrotizing Enterocolitis (NEC) is the most common gastrointestinal emergency in neonates and occurs more frequently in premature infants than term infants. We propose that fetal fibroblasts are inherently more responsive to stress and contribute to increased inflammation. Method: Primary human FIF and NNIF cell lines were exposed to E. coli LPS for 24 hours and the amount of IL-8 in the supernatants was determined by quantitative E

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Yin, X. J., H. S. Lee, Y. H. Lee, et al. "Cats cloned from fetal and adult somatic cells by nuclear transfer." Reproduction 129, no. 2 (2005): 245–49. http://dx.doi.org/10.1530/rep.1.00403.

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This work was undertaken in order to study the developmental competence of nuclear transfer (NT ) into cat embryos using fetal fibroblast and adult skin fibroblast cells as donor nuclei. Oocytes were recovered by mincing the ovaries in Hepes-buffered TCM199 and selecting the cumulus oocyte complexes (COCs) with compact cumulus cell mass and dark color. Homogenous ooplasm was cultured for maturation in TCM199+10% fetal bovine serum (FBS) for 12 h and used as a source of recipient cytoplast for exogenous somatic nuclei. In experiment 1, we evaluated the effect of donor cell type on the reconstru

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Götte, Martin, David D. Sofeu Feugaing, and Hans Kresse. "Age-Related Molecular Polymorphism of the Heterodimeric Proteoglycan Bisdermican." Scientific World JOURNAL 4 (2004): 1017–26. http://dx.doi.org/10.1100/tsw.2004.204.

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Bisdermican (PG760) is a large, heterodimeric, dermatan sulfate proteoglycan found in selected basement membranes, smooth muscle cell layers, and different extracellular matrices. Age-dependent and developmentally regulated alterations in glycosaminoglycan structure and quantity have been shown to be functionally relevant for a number of physiological and pathological processes. Bisdermican was purified from human skin fibroblast cultures of different age and confluency. Following β-elimination, glycosaminoglycan chains were analyzed by Sephacryl-S-300 chromatography. Glycosaminoglycan chains

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Hussain, Naveed, Fengying Wu, Constance Christian, and Mitchell J. Kresch. "Hyperoxia inhibits fetal rat lung fibroblast proliferation and expression of procollagens." American Journal of Physiology-Lung Cellular and Molecular Physiology 273, no. 4 (1997): L726—L732. http://dx.doi.org/10.1152/ajplung.1997.273.4.l726.

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The direct effects of hyperoxia on collagen production by fetal lung fibroblasts are unknown and would be important to the understanding of the molecular mechanisms involved in bronchopulmonary dysplasia in premature infants. We studied the effect of hyperoxia on 1) proliferation, 2) mRNA levels for type I and III procollagens, and 3) net collagen production in primary cultures of fetal rat lung fibroblasts. Fibroblasts from 19-day-old rat fetuses (term is 22 days) were obtained. Test plates were incubated in hyperoxia and controls in room air for varying time periods. Cell viability in both c

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Fistal, E. Ya, A. G. Popandopulo, V. V. Soloshenko, et al. "About the effectiveness of cell technologies in extensive soft tissue defects plasty." Bulletin of the Russian Military Medical Academy 22, no. 3 (2020): 88–92. http://dx.doi.org/10.17816/brmma50540.

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Abstract. Fetal fibroblast culture transplantation results were evaluated in the treatment of 18 burn victims. Comparison group consisted of 18 burn patients received medical care without cellular technologies utilization. The main comparison parameters in the study groups: the timing of the first stage of autodermoplasty; the number of autodermoplasties during the treatment; hospitalization duration; the newly formed epidermis area estimation. Fetal fibroblast culture transplantation in burn patients with extensive skin defects was performed on average 14,883,56 days after the injury. The tim

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Knoll, AB, T. Brockmeyer, R. Chevalier, K. Zscheppang, HC Nielsen, and CE Dammann. "Adult Rat Bone Marrow-Derived Stem Cells Promote Late Fetal Type II Cell Differentiation in a Co-Culture Model." Open Respiratory Medicine Journal 7, no. 1 (2013): 46–53. http://dx.doi.org/10.2174/1874306401307010046.

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Bronchopulmonary dysplasia develops in preterm infants due to a combination of lung immaturity and lung injury. Cultured pluripotent bone marrow stem cells (BMSC) are known to reduce injury and induce repair in adult and in immature lungs, possibly through paracrine secretion of soluble factors. The paracrine relationship between BMSC and primary fetal lung epithelial type II cells is unknown. We determined the effects of BMSC on type II cell and fibroblast behavior using an in vitro co-culture model. Rat BMSC were isolated and co-cultured with primary fetal E21 rat type II cells or lung fibro

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Fan, Z., M. Regouski, M. Brandsrud, et al. "206 Towards the generation of transchromosomic goats for the production of fully human immunoglobulin." Reproduction, Fertility and Development 31, no. 1 (2019): 228. http://dx.doi.org/10.1071/rdv31n1ab206.

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We have previously reported that inactivation of endogenous immunoglobulin (Ig) genes can enhance the production of human polyclonal antibodies (hpAb) in transchromosomic (Tc) cattle containing a human artificial chromosome (HAC) comprising the entire human Ig gene repertoire. Goats offer the advantages of having a shorter gestation period and growing to adult size much faster than larger ungulates. Therefore, based upon our previously established Tc cattle platform, in this study, we aimed to generate immunoglobulin lambda light chain knockout (IGLλ −/− )/HAC Tc goats expressing hpAb using CR

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Senior, R. M., G. L. Griffin, H. D. Perez, and R. O. Webster. "Human C5a and C5a des Arg exhibit chemotactic activity for fibroblasts." Journal of Immunology 141, no. 10 (1988): 3570–74. http://dx.doi.org/10.4049/jimmunol.141.10.3570.

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Abstract C5a and C5a des Arg are chemotactic factors for inflammatory cells but it is not known whether these agents are chemoattractants for fibroblasts. Accordingly, C5a, purified from zymosan-activated human, and C5a des Arg, prepared by incubating C5a with immobilized porcine carboxypeptidase B, were studied for fibroblast chemotactic activity. We observed that both C5a and C5a des Arg stimulated human skin fibroblasts and fetal bovine ligament fibroblasts to migrate in a concentration-dependent fashion, and that the migratory responses were similar in magnitude to the responses achieved w

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Shin, Y. M., S. M. Chang, B. C. Kim, C. S. Park, and D. I. Jin. "409 EFFICIENCY OF CO-TRANSFECTION OF TRANSGENE AND Neor GENE INTO GOAT AND PIG FETAL FIBROBLASTS." Reproduction, Fertility and Development 19, no. 1 (2007): 320. http://dx.doi.org/10.1071/rdv19n1ab409.

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Transgenic animals can be generated by nuclear transfer with genetically modified somatic cells in which the essential procedure of transgene transfection is required. Most transgene vectors are constructed to contain transgene and drug-resistant genes to enrich for somatic cells in which transgene integration has occurred. However, construction of transgene vectors along with drug-resistant genes may not be easy, due to inappropriate restriction sites. Therefore, in this study, two separate constructs, human tPA cDNA fused to β-casein promoter sequence as a transgene vector and neomycin-resis

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Samiec, M., M. Skrzyszowska, M. Bochenek, D. Lipinski, and R. Slomski. "83 IN VITRO DEVELOPMENTAL ABILITIES OF PORCINE CLONED EMBRYOS RECONSTITUTED WITH CELL NUCLEI OF GENETICALLY TRANSFORMED FETAL FIBROBLASTS CYTOMETRICALLY DIAGNOSED ON CELL CYCLE AND APOPTOSIS." Reproduction, Fertility and Development 19, no. 1 (2007): 159. http://dx.doi.org/10.1071/rdv19n1ab83.

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The important factor that determines the development of mammalian cloned embryos is structuro-functional quality of nuclear donor cells. Analysis of nuclear DNA (nDNA) content of somatic cells undergoing apoptosis has become one of the most common methods for single-parameter flow cytometric measurement of this process. Apoptosis assessment is performed by quantification of hypodiploid cells. The aim of our study was to examine the in vitro developmental potential of porcine nuclear transfer (NT) embryos reconstituted with non-apoptotic fetal fibroblast cells expressing the eGFP transgene. The

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Cartel, Nicholas J., and Martin Post. "Abrogation of apoptosis through PDGF-BB-induced sulfated glycosaminoglycan synthesis and secretion." American Journal of Physiology-Lung Cellular and Molecular Physiology 288, no. 2 (2005): L285—L293. http://dx.doi.org/10.1152/ajplung.00275.2004.

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Platelet-derived growth factor (PDGF)-BB-stimulated glycosaminoglycan (GAG) synthesis/secretion in fetal lung fibroblasts is dependent on sequential activation of the PDGF β-receptor, phosphatidylinositol 3-kinase (PI3K), the serine/threonine kinase Akt-1,2, and the GTPase Rab3D. Because the Akt pathway has been implicated in cell survival mechanisms, we investigated whether the pathway regulating GAG synthesis/secretion was antiapoptotic. PDGF-BB treatment protected fetal lung fibroblasts against serum starvation-induced apoptosis, whereas wortmannin, an inhibitor of PI3K, abrogated this prot

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Aguiar, Alexis, Melissa Galinato, Maite’ Bradley Silva, Bryant Toth, Michael A. McVoy, and Laura Hertel. "Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts." Viruses 13, no. 12 (2021): 2355. http://dx.doi.org/10.3390/v13122355.

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Only a handful of cell types, including fibroblasts, epithelial, and endothelial cells, can support human cytomegalovirus (CMV) replication in vitro, in striking contrast to the situation in vivo. While the susceptibility of epithelial and endothelial cells to CMV infection is strongly modulated by their anatomical site of origin, multiple CMV strains have been successfully isolated and propagated on fibroblasts derived from different organs. As oral mucosal cells are likely involved in CMV acquisition, we sought to evaluate the ability of infant labial fibroblasts to support CMV replication,

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Zhou, H. M., and Y. Chen. "104 DEVELOPMENTAL POTENTIAL OF CLONED EMBRYOS FROM ADULT AND FETAL OVINE SOMATIC CELLS." Reproduction, Fertility and Development 19, no. 1 (2007): 169. http://dx.doi.org/10.1071/rdv19n1ab104.

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This study reconstructed embryos using adult and fetal skin fibroblast cells as donor karyoplasts and ovine enucleated oocytes as recipient cytoplasts for comparing the developmental potential of the reconstructed embryos. Ovine ovaries were collected at a local slaughterhouse and the cumulus–oocyte complexes (COCs) were extracted from antral follicles 2 to 5 mm in diameter. A group of 20 to 30 COCs were matured in a 50-�L microdrop of maturation medium that was composed of TCM-199 supplemented with 20% FBS, 10 �g mL-1 FSH, 20 �g mL-1 LH, and 1.5 �g mL-1 17β-estradiol under mineral oil in a 35

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Akers, Ian A., Maddy Parsons, Michael R. Hill, et al. "Mast cell tryptase stimulates human lung fibroblast proliferation via protease-activated receptor-2." American Journal of Physiology-Lung Cellular and Molecular Physiology 278, no. 1 (2000): L193—L201. http://dx.doi.org/10.1152/ajplung.2000.278.1.l193.

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Mast cells play a potentially important role in fibroproliferative diseases, releasing mediators including tryptase that are capable of stimulating fibroblast proliferation and procollagen synthesis. The mechanism by which tryptase stimulates fibroblast proliferation is unclear, although recent studies suggest it can activate protease-activated receptor (PAR)-2. We therefore investigated the role of PAR-2 in tryptase-induced proliferation of human fetal lung and adult lung parenchymal and airway fibroblasts and, for comparative purposes, adult dermal fibroblasts. Tryptase (0.7–70 mU/ml) induce

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Wright, Clyde J., Tiangang Zhuang, Ping La, Guang Yang та Phyllis A. Dennery. "Hyperoxia-induced NF-κB activation occurs via a maturationally sensitive atypical pathway". American Journal of Physiology-Lung Cellular and Molecular Physiology 296, № 3 (2009): L296—L306. http://dx.doi.org/10.1152/ajplung.90499.2008.

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NF-κB activation is exaggerated in neonatal organisms after oxidant and inflammatory insults, but the reason for this and the downstream effects are unclear. We hypothesized that specific phosphorylation patterns of IκBα could account for differences in NF-κB activation in hyperoxia-exposed fetal and adult lung fibroblasts. After exposure to hyperoxia (>95% O2), nuclear NF-κB binding increased in fetal, but not adult, lung fibroblasts. Unique to fetal cells, phosphorylation of IκBα on tyrosine 42, rather than serine 32/36 as seen in TNF-α-exposed cells, preceded NF-κB nuclear translocation.

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Torday, J. S., M. Post, and B. T. Smith. "Compartmentalization of 11-oxidoreductase within fetal lung alveolus." American Journal of Physiology-Cell Physiology 249, no. 1 (1985): C173—C176. http://dx.doi.org/10.1152/ajpcell.1985.249.1.c173.

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The role of 11-oxidoreductase in the cellular process of fetal lung surfactant production and its localization within the alveolar domain have been investigated. In organotypic cultures of fetal rat lung, cortisol and cortisone markedly stimulate saturated phosphatidylcholine synthesis by the alveolar type II cell; a 10-fold excess of 11-ketoprogesterone blocks the bioactivity of cortisone. Both cortisol and cortisone also stimulate fibroblast-pneumonocyte factor production, whereas 11-ketoprogesterone blocks the effect of cortisone, but not of cortisol, suggesting that cortisone stimulation o

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Togo, Shinsaku, Xiangde Liu, Xingqi Wang та ін. "PDE4 inhibitors roflumilast and rolipram augment PGE2 inhibition of TGF-β1-stimulated fibroblasts". American Journal of Physiology-Lung Cellular and Molecular Physiology 296, № 6 (2009): L959—L969. http://dx.doi.org/10.1152/ajplung.00508.2007.

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Fibrotic diseases are characterized by the accumulation of extracellular matrix together with distortion and disruption of tissue architecture. Phosphodiesterase (PDE)4 inhibitors, by preventing the breakdown of cAMP, can inhibit fibroblast functions and may be able to mitigate tissue remodeling. Transforming growth factor (TGF)-β1, a mediator of fibrosis, can potentially modulate cAMP by altering PGE2 metabolism. The present study assessed whether PDE4 inhibitors functionally antagonize the profibrotic activity of fibroblasts stimulated by TGF-β1. The PDE4 inhibitors roflumilast and rolipram

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Kwon, H. S., H. J. Oh, D. H. Lee, D. E. Kim, S. K. Kang, and B. C. Lee. "285 GENERATION OF CANINE INDUCED PLURIPOTENT STEM CELLS FROM CANINE FETAL FIBROBLAST AND ADULT FIBROBLAST OF CLONED DOG." Reproduction, Fertility and Development 25, no. 1 (2013): 290. http://dx.doi.org/10.1071/rdv25n1ab285.

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Induced pluripotent stem cells (iPSC) derived from a patient’s fibroblasts have been used as fine resources for studying disease mechanisms and therapeutic strategies. The dog is considered invaluable in human disease research because its genetic diseases are strikingly similar to those of human. Therefore, we generated cloned dogs and transgenic cloned dogs via somatic cell nuclear transfer. In this study, we tried to derive canine iPSCs from canine fibroblasts to establish a way to make iPSC from skin fibroblasts of transgenic cloned dogs. We isolated canine fetal fibroblast (FF) from normal

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Heidari, B., A. Shirazi, P. Tajic, et al. "Effect of donor cell age on development of ovine nuclear transfer embryos in vitro." Zygote 18, no. 4 (2010): 331–38. http://dx.doi.org/10.1017/s0967199409990360.

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SummaryThe effects of the age of cell donor animal on in vitro development of ovine nuclear transfer (NT) embryos were investigated. Somatic donor cells were obtained from two different sources: (1) adult cells (adult fibroblast cells; AFC and adult cumulus cells; ACC); and (2) fetal fibroblasts (40-day-old; FFC-40 and 65-day-old; FFC-65). The fibroblast cell lines were used for NT procedures within 4–13 subpassages. While the cumulus cells were used as non-cultured (fresh) cells. The in vitro matured abattoir-derived oocytes were considered as recipients. No differences in the rates of fusion

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Kohyama, Tadashi, Xiangde Liu, Hui Jung Kim, et al. "Prostacyclin analogs inhibit fibroblast migration." American Journal of Physiology-Lung Cellular and Molecular Physiology 283, no. 2 (2002): L428—L432. http://dx.doi.org/10.1152/ajplung.00432.2001.

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The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. Prostacyclin (PGI2) is a potent mediator in the coagulation and inflammatory processes. The aim of this study was to investigate the effect of PGI2on chemotaxis of human fetal lung fibroblasts (HFL-1). Using the blind well chamber technique, we found that the PGI2analog carbaprostacyclin (10−6M) inhibited HFL-1 chemotaxis to human plasma fibronectin (20 μg/ml) 58.0 ± 13.2% ( P

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Martinez, Javier, Teresa Sanchez, and Juan J. Moreno. "Role of prostaglandin H synthase-2-mediated conversion of arachidonic acid in controlling 3T6 fibroblast growth." American Journal of Physiology-Cell Physiology 273, no. 5 (1997): C1466—C1471. http://dx.doi.org/10.1152/ajpcell.1997.273.5.c1466.

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The specific role(s) of arachidonic acid (AA) and its metabolites in the signaling pathways that regulated fibroblast growth was studied. A Western blot analysis demonstrated that prostaglandin H synthase-2 (PGHS-2) was expressed by 3T6 fibroblast cultures in RPMI 1640 supplemented with fetal calf serum (10%). Dexamethasone, which inhibits AA release and PGHS-2 expression, significantly reduced cell proliferation. Ketoprofen, a dual cyclooxygenase inhibitor, and CGP-28238, a specific PGHS-2 inhibitor, reduced fibroblast proliferation in a dose-dependent manner. These drugs also reduced [3H]thy

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Kim, So Hee, Bokyung Kim, Jung Hak Kim, et al. "L-myc Gene Expression in Canine Fetal Fibroblasts Promotes Self-Renewal Capacity but Not Tumor Formation." Cells 10, no. 8 (2021): 1980. http://dx.doi.org/10.3390/cells10081980.

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Canines are useful in mammalian preclinical studies because they are larger than rodents and share many diseases with humans. Canine fetal fibroblast cells (CFFs) are an easily accessible source of somatic cells. However, they are easily driven to senescence and become unusable with continuous in vitro culture. Therefore, to overcome these deficiencies, we investigated whether tetracycline-inducible L-myc gene expression promotes self-renewal activity and tumorigenicity in the production of induced conditional self-renewing fibroblast cells (iCSFCs). Here, we describe the characterization of a

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Zinman, H. M., M. G. Joneja, P. Davies, and B. T. Smith. "Cell Culture of Embryonic Chick Duodenal Cells." Journal of Pediatric Gastroenterology and Nutrition 4, no. 1 (1985): 107–17. http://dx.doi.org/10.1002/j.1536-4801.1985.tb08798.x.

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A method for the primary cell culture of trypsin‐dissociated embryonic chick duodenum is described. Both heterotypic (epithelial cells and fibroblasts together) and homotypic (highly enriched cultures of epithelial cells or fibroblasts alone) cell cultures were established. Dispersed duodenal epithelial cells and fibroblasts grown in 10% fetal bovine serum (FBS) spontaneously aggregated and proliferated as a bilayer of cells with the epithelial cells growing on top of the fibroblasts. Changing the serum supplement to 6% chicken serum (CS) and 4% FBS when the fibroblast monolayer reached conflu

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Liu, Yaowei, Yujie Zhang, Maosheng Cui, Xiangfei Zhao, Mingzhu Sun, and Xin Zhao. "A Cell’s Viscoelasticity Measurement Method Based on the Spheroidization Process of Non-Spherical Shaped Cell." Sensors 21, no. 16 (2021): 5561. http://dx.doi.org/10.3390/s21165561.

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The mechanical properties of biological cells, especially the elastic modulus and viscosity of cells, have been identified to reflect cell viability and cell states. The existing measuring techniques need additional equipment or operation condition. This paper presents a cell’s viscoelasticity measurement method based on the spheroidization process of non-spherical shaped cell. The viscoelasticity of porcine fetal fibroblast was measured. Firstly, we introduced the process of recording the spheroidization process of porcine fetal fibroblast. Secondly, we built the viscoelastic model for simula

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Han, Lu, Tom Walter, Joseph Beaudet, Caroline Everett, Gustavo Leone, and Michael Ostrowski. "Abstract A044: Persistence of fetal splanchnic gene signature defines a tumor-restraining fibroblast subtype in pancreatic cancer." Cancer Research 84, no. 17_Supplement_2 (2024): A044. http://dx.doi.org/10.1158/1538-7445.pancreatic24-a044.

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Abstract The pancreas is composed of the epithelial and mesenchymal cells. While mesenchymal fibroblasts are a minor component of the normal pancreas, fibroblast population expands drastically during tumorigenesis. In pancreatic ductal adenocarcinoma (PDAC), cancer associated fibroblasts (CAFs) play critical and complex roles in the tumor microenvironment. This study sought to define the origin, heterogeneity and function of pancreatic cancer associated fibroblasts. Recently we performed a series of lineage tracing studies in genetically engineered mouse models. This identified the splanchnic

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Caniggia, I., and M. Post. "Differential effect of platelet-derived growth factor on glycosaminoglycan synthesis by fetal rat lung cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 263, no. 4 (1992): L495—L500. http://dx.doi.org/10.1152/ajplung.1992.263.4.l495.

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Lung morphogenesis is in part regulated by extracellular matrix (ECM), and cytokines may indirectly control lung development via modulation of ECM. In the present study, we investigated the effect of different platelet-derived growth factor (PDGF) isoforms AA, AB, and BB on the synthesis of glycosaminoglycans (GAG) by fetal rat lung cells. Independent of gestational age, PDGF-BB, but not PDGF-AA or -AB, stimulated GAG synthesis of fetal lung fibroblasts. In contrast, GAG synthesis by epithelial cells was not affected by any of the PDGF molecules. The stimulatory effect of PDGF-BB on fibroblast

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Goldsmith, K. T., R. B. Gammon, and R. I. Garver. "Modulation of bFGF in lung fibroblasts by TGF-beta and PDGF." American Journal of Physiology-Lung Cellular and Molecular Physiology 261, no. 6 (1991): L378—L385. http://dx.doi.org/10.1152/ajplung.1991.261.6.l378.

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Growth factors produced by alveolar macrophages are thought to promote the fibroblast proliferation within interstitial spaces of fibrotic lungs. This study investigated the possibility that the macrophage-produced growth factors might modulate the expression of basic fibroblast growth factor (bFGF) by lung fibroblasts. To evaluate this question, bFGF gene expression and protein production were evaluated in normal adult human lung fibroblast cell lines. Under normal culture conditions, the fibroblasts expressed the bFGF gene as two major transcripts (7.1, 3.7 kb). The addition of fetal calf se

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Kohyama, Tadashi, Ronald F. Ertl, Vincenzo Valenti, et al. "Prostaglandin E2 inhibits fibroblast chemotaxis." American Journal of Physiology-Lung Cellular and Molecular Physiology 281, no. 5 (2001): L1257—L1263. http://dx.doi.org/10.1152/ajplung.2001.281.5.l1257.

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Fibroblasts are the major source of extracellular connective tissue matrix, and the recruitment, accumulation, and stimulation of these cells are thought to play important roles in both normal healing and the development of fibrosis. Prostaglandin E2(PGE2) can inhibit this process by blocking fibroblast proliferation and collagen production. The aim of this study was to investigate the inhibitory effect of PGE2 on human plasma fibronectin (hFN)- and bovine bronchial epithelial cell-conditioned medium (BBEC-CM)-induced chemotaxis of human fetal lung fibroblasts (HFL1). Using the Boyden blind we

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Caneparo, Christophe, Stéphane Chabaud, Julie Fradette, and Stéphane Bolduc. "Evaluation of a Serum-Free Medium for Human Epithelial and Stromal Cell Culture." International Journal of Molecular Sciences 23, no. 17 (2022): 10035. http://dx.doi.org/10.3390/ijms231710035.

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Over the past decade, growing demand from many domains (research, cosmetics, pharmaceutical industries, etc.) has given rise to significant expansion of the number of in vitro cell cultures. Despite the widespread use of fetal bovine serum, many issues remain. Among them, the whole constitution of most serums remains unknown and is subject to significant variations. Furthermore, the presence of potential contamination and xenogeny elements is challenging for clinical applications, while limited production is an obstacle to the growing demand. To circumvent these issues, a Serum-Free Medium (SF

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Jena, M. K., D. Malakar, A. K. De, S. Garg, and Y. S. Akshey. "59 PRODUCTION OF HANDMADE CLONED GOAT EMBRYOS WITH TWO TYPES OF DONOR CELLS AND CULTURED IN THREE MEDIA." Reproduction, Fertility and Development 22, no. 1 (2010): 187. http://dx.doi.org/10.1071/rdv22n1ab59.

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The study was carried out to see the developmental efficiency of handmade cloned goat embryos with 3 different media: RVCL (Research Vitro Cleave, Cook, Brisbane, Australia), EDM (Embryo Development Media) and modified SOF (mSOF) and 2 types of donor cells: fetal fibroblast and adult fibroblast. Oocytes were isolated from abattoir goat ovaries, matured in maturation medium, and incubated in 5% CO2 in air at 38.5°C for 24 h. Then, the oocytes were made cumulus free by treatment with hyaluronidase (0.5 mg mL-1) and zona free by pronase (2 mg mL-1). Protrusion cone formation in oocytes was found

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Sucre, Jennifer M. S., Preethi Vijayaraj, Cody J. Aros та ін. "Posttranslational modification of β-catenin is associated with pathogenic fibroblastic changes in bronchopulmonary dysplasia". American Journal of Physiology-Lung Cellular and Molecular Physiology 312, № 2 (2017): L186—L195. http://dx.doi.org/10.1152/ajplung.00477.2016.

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Bronchopulmonary dysplasia (BPD) is a common complication of premature birth. The histopathology of BPD is characterized by an arrest of alveolarization with fibroblast activation. The Wnt/β-catenin signaling pathway is important in early lung development. When Wnt signaling is active, phosphorylation of β-catenin by tyrosine kinases at activating sites, specifically at tyrosine 489 (Y489), correlates with nuclear localization of β-catenin. We examined fetal lung tissue, lung tissue from term newborns, and lung tissue from infants who died with BPD; we found nuclear β-catenin phosphorylation a

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Hong, S. G., G. Jang, M. K. Kim, et al. "36 CLONED BEAGLE DOGS DERIVED FROM FETAL FIBROBLASTS BY NUCLEAR TRANSFER." Reproduction, Fertility and Development 20, no. 1 (2008): 98. http://dx.doi.org/10.1071/rdv20n1ab36.

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Somatic cell nuclear transfer (SCNT) has been successfully performed in various mammals including sheep, cow, pig, and mouse using a variety of somatic cell types as nuclear donors. Several reports of livestock SCNT indicate that fetal fibroblasts are superior to adult fibroblasts as donor cells. In canine SCNT, however, only adult ear fibroblasts have been used as donor cells (Lee et al. 2005 Nature 436, 641; Jang et al. 2007 Theriogenology 67, 941–947). Accordingly, in the present study, we evaluated the ability of canine fetal fibroblasts to support fetal development to term after nuclear t

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Kragh, P. M., Y. Du, J. Li, Y. Zhang, L. Bolund, and G. Vajta. "56 TRANSGENESIS BY HANDMADE CLONING USING EGFP-TRANSFECTED YUCATAN FIBROBLASTS." Reproduction, Fertility and Development 19, no. 1 (2007): 146. http://dx.doi.org/10.1071/rdv19n1ab56.

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Somatic cell nuclear transfer (SCNT) offers the possibility of pig transgenesis. Importantly, genetic manipulations can be performed in cells isolated from special breeds followed by SCNT into enucleated oocytes isolated from slaughterhouse ovaries. In the present study, we established production of Yucatan blastocysts by the handmade cloning (HMC) technique using non-transgenic fibroblasts from the Yucatan miniature pig, and produced transgenic blastocysts using enhanced green fluorescent protein (EGFP)-positive Yucatan fetal fibroblasts. For transgenesis, Yucatan fibroblasts from a 40-day ol

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Li, Xuyan, Tianfei Yu, Ming Li, Youqi Wang, Bo Meng, and Yanshuang Mu. "NANOG improves type I collagen expression in human fetal scleral fibroblasts." Archives of Biological Sciences 71, no. 1 (2019): 63–70. http://dx.doi.org/10.2298/abs180711048l.

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Human fetal scleral fibroblasts (HFSFs) are components of the sclera and play important roles in its structure and function. In myopia, scleral remodeling reduces collagen fibers and the sclera begins to thin. NANOG is a key transcription factor essential for pluripotent and self-renewing phenotypes of undifferentiated embryonic stem cells. To determine whether NANOG improves human fetal scleral fibroblast quality and the underlying mechanisms in these cells, we established stable NANOG-overexpressing HFSFs. We studied type I collagen (COL1A 1) and Rho-associated coiled-coil protein kinase 1 (

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