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Academic literature on the topic 'Genotypic Analysis of m morganii'
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Journal articles on the topic "Genotypic Analysis of m morganii"
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Zubair, Mohammad, and Ibrahim Mohammad. "Interrelationship of Extended Spectrum Beta-Lactamase Producers and Biofilm Formation among the Gram-Negative Bacteria from Tabuk, KSA." Open Access Macedonian Journal of Medical Sciences 11, A (2023): 15–22. http://dx.doi.org/10.3889/oamjms.2023.11101.
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AIM: The present study investigates the production of extended-spectrum beta-lactamases (ESBL) and the formation of biofilm among different bacterial pathogens. METHODS: The study conducted prospective analysis on bacteria isolates (Gram-negative) from patients who have diagnosed with infections with bacteria between October 2020 and January 2022. RESULTS: The results showed that there were 53 biofilm producers in Escherichia coli. In contrast, Pseudomonas aeruginosa was observed to have the highest percentage, with 32/40 (80%) isolates being biofilm producers. The least number of isolates were Morganella morganii (n = 2) with two (100%) biofilm producers. The resistance in the biofilm positive isolates was high compared with biofilm negative. About 88% of phenotypic ESBL-positive isolates were biofilm producers, and 97% of cefotaxime-resistant biofilm-positive isolates were genotypic positive for CTX-M, TEM, and SHV genes. CONCLUSION: The present study has shown that protection against antibiotics through mucus production is possible due to bacteria’s reduced metabolic activity and diffusion of antibiotics across the biofilm matrix. In this study, all the bacterial strains of E. coli and Klebsiella pneumoniae were reported to be MDR and competent for establishing biofilm.
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Dropa, Milena, Livia C. Balsalobre, Nilton Lincopan, et al. "Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil." Revista do Instituto de Medicina Tropical de São Paulo 51, no. 4 (2009): 203–9. http://dx.doi.org/10.1590/s0036-46652009000400005.
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Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment.
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Emborg, Jette, Paw Dalgaard, and Peter Ahrens. "Morganella psychrotolerans sp. nov., a histamine-producing bacterium isolated from various seafoods." International Journal of Systematic and Evolutionary Microbiology 56, no. 10 (2006): 2473–79. http://dx.doi.org/10.1099/ijs.0.64357-0.
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Mesophilic Morganella morganii (n=6) and psychrotolerant M. morganii-like isolates from various seafoods (n=13), as well as clinical M. morganii isolates (n=3), were characterized by using a polyphasic approach including multi-locus sequencing. Based on the phylogenetic analysis, the 22 strains were divided into two distinct groups comprising mesophilic and psychrotolerant isolates, respectively. This classification was supported by DNA–DNA hybridization studies, whereby a psychrotolerant isolate (strain U2/3T) showed 41.0 and 17.8 % relatedness to the type strains of the mesophilic species Morganella morganii subsp. morganii (strain LMG 7874T) and Morganella morganii subsp. sibonii (strain DSM 14850T), respectively. Analysis of the 16S rRNA gene sequences showed a similarity of 98.6 % between mesophilic and psychrotolerant isolates. However, fragments of seven protein-encoding housekeeping genes (atpD, dnaN, gyrB, hdc, infB, rpoB and tuf) all showed less than 90.9 % sequence similarity between the two groups. The psychrotolerant isolates grew at 0–2 °C and also differed from the mesophilic M. morganii isolates with respect to growth at 37 °C and in 8.5 % (w/v) NaCl and fermentation of d-galactose. The psychrotolerant strains appear to represent a novel species, for which the name Morganella psychrotolerans sp. nov. is proposed. The type strain is U2/3T (=LMG 23374T=DSM 17886T).
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Luo, Xingwei, Yajun Zhai, Dandan He, et al. "Molecular characterization of a novel bla CTX-M-3-carrying Tn6741 transposon in Morganella morganii isolated from swine." Journal of Medical Microbiology 69, no. 8 (2020): 1089–94. http://dx.doi.org/10.1099/jmm.0.001235.
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Introduction. The bla CTX-M-3 gene has rarely been reported in Morganella morganii strains and its genetic environment has not yet been investigated. Aim. To identify the bla CTX-M-3 gene in M. morganii isolated from swine and characterize its genetic environment. Methodology. A M. morganii isolate (named MM1L5) from a deceased swine was identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and subjected to antimicrobial susceptibility testing. The bla genes were detected and then the genetic location and environment of bla CTX-M-3 were investigated by Southern blot and PCR mapping, respectively. The M. morganii bla CTX-M-3 gene was cloned and expressed in Escherichia coli . Results. Isolate MM1L5 harboured the bla CTX-M-3 and bla TEM-1 genes. The bla CTX-M-3 gene, located on the chromosome, was co-carried with an IS26 and bla TEM-1 gene by a novel 6361 bp IS26-flanked composite transposon, designated Tn6741. This transposon consisted of a novel bla CTX-M-3-containing module, IS26-ΔISEcp1-bla CTX-M-3-Δorf477-IS26 (named Tn6710), and a bla TEM-1-containing module, IS26-Δorf477-bla TEM-1-tnpR-IS26, differing from previous reports. Phylogenetic analysis showed a significant variation based on the sequence of Tn6741, as compared to those of other related transposons. Interestingly, although the cloned bla CTX-M-3 gene could confer resistance to ceftiofur, cefquinome, ceftriaxone and cefotaxime, one amino acid substitution (Ile-142-Thr) resulted in a significant reduction of resistance to these antimicrobials. Conclusion. This is the first time that bla CTX-M-3 has been identified on a chromosome from a M. morganii isolate. Furthermore, the bla CTX-M-3 gene was located with an IS26 element and bla TEM-1 gene on a novel IS26-flanked composite transposon, Tn6741, suggesting that Tn6741 might act as a reservoir for the bla CTX-M-3 and bla TEM-1 genes and may become an important vehicle for their dissemination among M. morganii .
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Sun, Zhenxiao, Yi Zhang, Xinping Lin, Sufang Zhang, Yingxi Chen, and Chaofan Ji. "Inhibition Mechanism of Lactiplantibacillus plantarum on the Growth and Biogenic Amine Production in Morganella morganii." Foods 12, no. 19 (2023): 3625. http://dx.doi.org/10.3390/foods12193625.
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Morganella morganii, a spoilage bacterium in fermented foods, produces harmful biogenic amines (BAs). Although Lactiplantibacillus plantarum is widely used to inhibit spoilage bacteria, the inhibition pattern and inhibition mechanism of M. morganii by Lpb. plantarum are not well studied. In this study, we analysed the effects of the addition of Lpb. plantarum cell-free supernatant (CFS) on the growth and BA accumulation of M. morganii and revealed the mechanisms of changes in different BAs by using RNA sequencing transcriptome analysis. The results showed that Lpb. plantarum CFS could significantly inhibit M. morganii BAs in a weak acid environment (pH 6), and the main changes were related to metabolism. Carbohydrate and energy metabolism were significantly down-regulated, indicating that Lpb. plantarum CFS inhibited the growth activity and decreased the BA content of M. morganii. In addition, the change in histamine content is also related to the metabolism of its precursor amino acids, the change in putrescine content may also be related to the decrease in precursor amino acid synthesis and amino acid transporter, and the decrease in cadaverine content may also be related to the decrease in the cadaverine transporter. The results of this study help to inhibit the accumulation of harmful metabolites in fermented foods.
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Taher, Eman M., Basima A. Abdullah, and Anmar A. AL Taie. "Phenotypic and genotypic detection of biofilm genes for<i> </i><i>Proteus, Morganella, Providencia</i> (PMP) bacterial group isolated from clinical samples of some hospitals in Mosul/Iraq." Journal of Applied and Natural Science 16, no. 4 (2024): 1719–30. https://doi.org/10.31018/jans.v16i4.5935.
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The three genera Proteus spp., Morganella spp., Providencia spp. (PMP) is a group of bacteria belonging to the Enterobacteriaceae family that is predominantly implicated in nosocomial infections within hospital environments. The research aimed to ascertain the frequency of PMP in various clinical samples from some hospitals by isolation and confirming the identification of bacteria from biofilms using Viteck-2 and phenotypic and genotypic determinants of biofilm genes. Out of 380 isolates from different clinical sources that were identified phenotypically (microscopic examination, culture, and biochemical tests) confirmed identification of bacteria from biofilms using Viteck-2 and genotypic determinants of biofilm genes DNA were extracted from twenty identified strains, the PCR products derived were utilized for sequencing. The Congo Red Agar and Microplate methods employed encompassed qualitative and quantitative approaches to analyze the formation of biofilms. In this approach, bacteria that develop black colonies were assessed as positive for biofilm formation.Proteus mirabilis showed strong producers (19.8%), Providencia stuartii (100%), and Morganella morganii produced pink colonies. The strains were assessed as non-producers and analyzed quantitatively using the microplate technique. which shows strong biofilm production in most PMP groups. Genetic detection of biofilm genes showed that the mrpA gene was identified in P. mirabilis strains utilizing the PCR technique as positive for all strains at (100%) but M. morganii at (25%), positive amplification for all strains (100%) for the rsm gene in P. mirabilis strains. P. stuartii gave positive detection for the fim A gene by utilizing the PCR technique as (100%). All genes were involved in biofilm formation.
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Barnaud, Guilene, Guillaume Arlet, Charlotte Verdet, Olivier Gaillot, Philippe H. Lagrange та Alain Philippon. "Salmonella enteritidis: AmpC Plasmid-Mediated Inducible β-Lactamase (DHA-1) with anampR Gene from Morganella morganii". Antimicrobial Agents and Chemotherapy 42, № 9 (1998): 2352–58. http://dx.doi.org/10.1128/aac.42.9.2352.
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ABSTRACT DHA-1, a plasmid-mediated cephalosporinase from a single clinicalSalmonella enteritidis isolate, conferred resistance to oxyimino-cephalosporins (cefotaxime and ceftazidime) and cephamycins (cefoxitin and moxalactam), and this resistance was transferable toEscherichia coli HB101. An antagonism was observed between cefoxitin and aztreonam by the diffusion method. Transformation of the transconjugant E. coli strain with plasmid pNH5 carrying the ampD gene (whose product decreases the level of expression of ampC) resulted in an eightfold decrease in the MIC of cefoxitin. A clone with the same AmpC susceptibility pattern with antagonism was obtained, clone E. coliJM101(pSAL2-ind), and its nucleotide sequence was determined. It contained an open reading frame with 98.7% DNA sequence identity with the ampC gene of Morganella morganii. DNA sequence analysis also identified a gene upstream of ampCwhose sequence was 97% identical to the partial sequence of the ampR gene (435 bp) from M. morganii. The gene encoded a protein with an amino-terminal DNA-binding domain typical of transcriptional activators of the LysR family. Moreover, the intercistronic region between the ampC andampR genes was 98% identical to the corresponding region from M. morganii DNA. AmpR was shown to be functional by enzyme induction and a gel mobility-shift assay. An ampGgene was also detected in a Southern blot of DNA from the S. enteritidis isolate. These findings suggest that this inducible plasmid-mediated AmpC type β-lactamase, DHA-1, probably originated from M. morganii.
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Al Sium, Syed Muktadir, Barna Goswami, Sanjana Fatema Chowdhury, et al. "An insight into the genome-wide analysis of bacterial defense mechanisms in a uropathogenic Morganella morganii isolate from Bangladesh." PLOS ONE 20, no. 1 (2025): e0313141. https://doi.org/10.1371/journal.pone.0313141.
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The gram-negative, facultative anaerobic bacterium Morganella morganii is linked to a number of illnesses, including nosocomial infections and urinary tract infections (UTIs). A clinical isolate from a UTI patient in Bangladesh was subjected to high-throughput whole genome sequencing and extensive bioinformatics analysis in order to gather knowledge about the genomic basis of bacterial defenses and pathogenicity in M. morganii. With an average nucleotide identity (ANI) of more than 97% similarity to a reference genome and phylogenetic analysis verified the isolate as M. morganii. Genome annotation identified 3,718 protein-coding sequences, including genes for metabolism, protein processing, stress response, energy, and membrane transport. The presence of biosynthetic gene clusters points to the isolate’s ability to create bioactive compounds, including antibiotics. Genomic islands contained genes for metal transporters, stress proteins, toxin proteins, and genes related to horizontal gene transfer. The beta-lactam resistance gene blaDHA was found using antimicrobial resistance (AMR) gene analysis across three databases. The virulence genes kdsA and cheY, which may be involved in chemotaxis and lipopolysaccharide production, were also available in the isolate, suggesting its high pathogenicity. The genome contained mobile genetic components and defense mechanisms, such as restriction modification and CRISPR-Cas systems, indicating the bacterium’s ability to defend itself against viral attacks. This thorough investigation sheds important light on M. morganii’s pathogenicity and adaptive tactics by revealing its genetic characteristics, AMR, virulence components, and defense mechanisms. For the development of targeted treatments and preventing the onset of resistance in clinical care, it is essential to comprehend these genetic fingerprints.
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Behera, Dibyajyoti Uttameswar, Sangita Dixit, Mahendra Gaur, et al. "Sequencing and Characterization of M. morganii Strain UM869: A Comprehensive Comparative Genomic Analysis of Virulence, Antibiotic Resistance, and Functional Pathways." Genes 14, no. 6 (2023): 1279. http://dx.doi.org/10.3390/genes14061279.
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Morganella morganii is a Gram-negative opportunistic Enterobacteriaceae pathogen inherently resistant to colistin. This species causes various clinical and community-acquired infections. This study investigated the virulence factors, resistance mechanisms, functional pathways, and comparative genomic analysis of M. morganii strain UM869 with 79 publicly available genomes. The multidrug resistance strain UM869 harbored 65 genes associated with 30 virulence factors, including efflux pump, hemolysin, urease, adherence, toxin, and endotoxin. Additionally, this strain contained 11 genes related to target alteration, antibiotic inactivation, and efflux resistance mechanisms. Further, the comparative genomic study revealed a high genetic relatedness (98.37%) among the genomes, possibly due to the dissemination of genes between adjoining countries. The core proteome of 79 genomes contains the 2692 core, including 2447 single-copy orthologues. Among them, six were associated with resistance to major antibiotic classes manifested through antibiotic target alteration (PBP3, gyrB) and antibiotic efflux (kpnH, rsmA, qacG; rsmA; CRP). Similarly, 47 core orthologues were annotated to 27 virulence factors. Moreover, mostly core orthologues were mapped to transporters (n = 576), two-component systems (n = 148), transcription factors (n = 117), ribosomes (n = 114), and quorum sensing (n = 77). The presence of diversity in serotypes (type 2, 3, 6, 8, and 11) and variation in gene content adds to the pathogenicity, making them more difficult to treat. This study highlights the genetic similarity among the genomes of M. morganii and their restricted emergence, mostly in Asian countries, in addition to their growing pathogenicity and resistance. However, steps must be taken to undertake large-scale molecular surveillance and to direct suitable therapeutic interventions.
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Ramya, D., A. Joseph Thatheyus, S. Jemima Balaselvi Juliana, N. Jennifer Michellin Kiruba, and Deborah Gnana Selvam A. "Physical characterization and kinetic studies of Zn (II) biosorption by Morganella morganii ACZ05." Water Science and Technology 85, no. 4 (2022): 970–86. http://dx.doi.org/10.2166/wst.2022.031.
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Abstract Through this investigation, we establish the mechanism and physical characterization of zinc (II) sequestration by Morganella morganii ACZ05 strain, which was isolated and characterized from soil polluted by effluents from electroplating industries. As far as we know, there is very little literature concerning zinc biosorption using an environmental strain of M. morganii. The SEM analysis shows the dark porous gaps in the aggregated cell-matrix of test bacterial biomass which is inferred as water channels usually seen in biofilms, as compared to metal-unexposed control. M. morganii is not known to produce biofilms unless in the rare nosocomial conditions. Here, SEM analysis shows the production of biofilms after exposure to zinc (II) at 500 ppm, which has not been previously reported. EDX analysis of bacterial biomass also specified the sorption of zinc (II) by the bacterial cells and the presence of new peaks for zinc in contrast to control. Both XRD and FTIR analysis observations strongly implicate the potential of physical adsorption as a mechanism for heavy metal resistance. Analysis of the cell surface by Atomic force microscopy and examination of the topography revealed cell aggregation occurs during biofilm production after zinc biosorption. Unlike other reports, regular models such as Langmuir isotherm and Freundlich isotherm were found insufficient to explain the physisorption of zinc (II) metal ions on complex multicomponent adsorbents such as the exopolymeric surface of the bacterial cells. However, adsorption kinetics of zinc (II) to the bacterial biomass was most effectively elucidated by a pseudo-second-order kinetic model, suggesting a certain kind of chemisorption that requires further study.
Dissertations / Theses on the topic "Genotypic Analysis of m morganii"
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Boinett, Christine J. "Phenotypic and genotypic analysis of blaCTX-M encoding plasmids isolated from bovine E. coli samples in the United Kingdom." Thesis, Royal Holloway, University of London, 2013. http://repository.royalholloway.ac.uk/items/d1b443bc-23f6-4cc5-953e-8672f2c79421/1/.
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The purpose of this study was to characterize blaCTX-M plasmids originating from bovine Escherichia coli and investigate their contribution to bacterial host fitness. In this study 52 bovine Escherichia coli strains collected between March and October 2007 encoding blaCTX-M, an extended spectrum β-lactamase (ESBL) gene conferring resistance to 3rd generation cephalosporins, were characterized. The majority of strains belonged to E. coli commensal phylogroups A and B1 expressing a multi-drug resistance (MDR) phenotype and harboured multiple plasmids of which 90% were transferred by conjugation. Transconjugants or transformants were made successfully from all 52 strains when selecting for resistance to cefotaxime. All plasmids were shown by PCR and sequence analysis to harbour blaCTX-M and nearly 80 % encoded multiple resistances. Plasmid sequence analysis of four plasmids encoding blaCTX-M-14b (IncI1-X1), -15 (IncFII-FIA-FIB) and -32 (IncX1 and IncB), identified genes necessary for stable plasmid maintenance and spread. Five representative plasmids encoding blaCTX-M-1, -15, -14b and -32 were assayed for their fitness impact upon the host. Efficiencies of β-lactam hydrolysis using whole cell extracts were determined in the same E. coli BL21 host strain with the most efficient encoded by blaCTX-M-14b and blaTEM-1 ESBL genes and least efficient encoded blaCTX-M-15 only. A 160 kb plasmid encoding 13 resistance genes was grown in the presence of 380 different metabolites and differences in metabolite utilisation between this and the plasmid-free BL21 strain determined. The plasmid-harbouring strain utilized less phosphor-sulphur compounds, suggesting the metabolic cost incurred by acquiring the plasmid may have implications of cellular utilization of alternative phosphate sources. There were no differences in growth was observed in nutrient rich media. The contribution of active efflux to resistance was investigated using L-phenylalanyl-L- 4 arginyl-b-naphthylamide (PAβN) in combination with ampicillin, cefotaxime or ceftazidime. Minimum inhibitory concentration (MIC) values were found to decrease ≥ 2 fold in the presence of the efflux pump inhibitor (EPI), in some cases becoming completely susceptible to ampicillin. This indicates that the possible use of EPIs in combination with previously failed antimicrobial drugs to potentially restore efficacy of treatment.