Academic literature on the topic 'Granule Disassembly'

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Journal articles on the topic "Granule Disassembly"

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Gwon, Youngdae, Brian A. Maxwell, Regina-Maria Kolaitis, Peipei Zhang, Hong Joo Kim, and J. Paul Taylor. "Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner." Science 372, no. 6549 (2021): eabf6548. http://dx.doi.org/10.1126/science.abf6548.

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Stress granules are dynamic, reversible condensates composed of RNA and protein that assemble in eukaryotic cells in response to a variety of stressors and are normally disassembled after stress is removed. The composition and assembly of stress granules is well understood, but little is known about the mechanisms that govern disassembly. Impaired disassembly has been implicated in some diseases including amyotrophic lateral sclerosis, frontotemporal dementia, and multisystem proteinopathy. Using cultured human cells, we found that stress granule disassembly was context-dependent: Specifically
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Jakobson, Christopher M., and Daniel F. Jarosz. "Metabolites control stress granule disassembly." Nature Cell Biology 23, no. 10 (2021): 1053–55. http://dx.doi.org/10.1038/s41556-021-00768-w.

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Panas, Marc D., Pavel Ivanov, and Paul Anderson. "Mechanistic insights into mammalian stress granule dynamics." Journal of Cell Biology 215, no. 3 (2016): 313–23. http://dx.doi.org/10.1083/jcb.201609081.

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The accumulation of stalled translation preinitiation complexes (PICs) mediates the condensation of stress granules (SGs). Interactions between prion-related domains and intrinsically disordered protein regions found in SG-nucleating proteins promote the condensation of ribonucleoproteins into SGs. We propose that PIC components, especially 40S ribosomes and mRNA, recruit nucleators that trigger SG condensation. With resolution of stress, translation reinitiation reverses this process and SGs disassemble. By cooperatively modulating the assembly and disassembly of SGs, ribonucleoprotein conden
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Dang, Yongjun, Nancy Kedersha, Woon-Kai Low та ін. "Eukaryotic Initiation Factor 2α-independent Pathway of Stress Granule Induction by the Natural Product Pateamine A". Journal of Biological Chemistry 281, № 43 (2006): 32870–78. http://dx.doi.org/10.1074/jbc.m606149200.

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Stress granules are aggregates of small ribosomal subunits, mRNA, and numerous associated RNA-binding proteins that include several translation initiation factors. Stress granule assembly occurs in the cytoplasm of higher eukaryotic cells under a wide variety of stress conditions, including heat shock, UV irradiation, hypoxia, and exposure to arsenite. Thus far, a unifying principle of eukaryotic initiation factor 2α phosphorylation prior to stress granule formation has been observed from the majority of experimental evidence. Pateamine A, a natural product isolated from marine sponge, was rec
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Xie, Wen, and Robert B. Denman. "Protein Methylation and Stress Granules: Posttranslational Remodeler or Innocent Bystander?" Molecular Biology International 2011 (February 24, 2011): 1–14. http://dx.doi.org/10.4061/2011/137459.

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Stress granules contain a large number of post-translationally modified proteins, and studies have shown that these modifications serve as recruitment tags for specific proteins and even control the assembly and disassembly of the granules themselves. Work originating from our laboratory has focused on the role protein methylation plays in stress granule composition and function. We have demonstrated that both asymmetrically and symmetrically dimethylated proteins are core constituents of stress granules, and we have endeavored to understand when and how this occurs. Here we seek to integrate
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Chen, Wenjun, Yabing Hu, Charles F. Lang, et al. "The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditis elegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle." Genetics 215, no. 2 (2020): 421–34. http://dx.doi.org/10.1534/genetics.120.303052.

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P granules are phase-separated liquid droplets that play important roles in the maintenance of germ cell fate in Caenorhabditis elegans. Both the localization and formation of P granules are highly dynamic, but mechanisms that regulate such processes remain poorly understood. Here, we show evidence that the VASA-like germline RNA helicase GLH-1 couples distinct steps of its ATPase hydrolysis cycle to control the formation and disassembly of P granules. In addition, we found that the phenylalanine-glycine-glycine repeats in GLH-1 promote its localization at the perinucleus. Proteomic analyses o
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Hird, S. N., J. E. Paulsen, and S. Strome. "Segregation of germ granules in living Caenorhabditis elegans embryos: cell-type-specific mechanisms for cytoplasmic localisation." Development 122, no. 4 (1996): 1303–12. http://dx.doi.org/10.1242/dev.122.4.1303.

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Germ granules are ribonucleoprotein particles that are thought to function in germline specification in invertebrates and possibly in vertebrates. In Caenorhabditis elegans, these structures, termed P granules, are partitioned to the germline P cells during the early embryonic divisions. By injecting a fluorescently labelled anti-P-granule antibody into the C. elegans germline syncitium, we followed P-granule segregation in live embryos using laser-scanning confocal microscopy. We show that, in early P cells (P0 and P1), P-granule partitioning is achieved primarily by their migration through t
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Chandler, D. E., M. Whitaker, and J. Zimmerberg. "High molecular weight polymers block cortical granule exocytosis in sea urchin eggs at the level of granule matrix disassembly." Journal of Cell Biology 109, no. 3 (1989): 1269–78. http://dx.doi.org/10.1083/jcb.109.3.1269.

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Recently, we have shown that high molecular weight polymers inhibit cortical granule exocytosis at total osmolalities only slightly higher than that of sea water (Whitaker, M., and J. Zimmerberg. 1987. J. Physiol. 389:527-539). In this study, we visualize the step at which this inhibition occurs. Lytechinus pictus and Strongylocentrotus purpuratus eggs were exposed to 0.8 M stachyose or 40% (wt/vol) dextran (average molecular mass of 10 kD) in artificial sea water, activated with 60 microM of the calcium ionophore A23187, and then either fixed with glutaraldehyde and embedded or quick-frozen a
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Maxwell, Brian A., Youngdae Gwon, Ashutosh Mishra, et al. "Ubiquitination is essential for recovery of cellular activities after heat shock." Science 372, no. 6549 (2021): eabc3593. http://dx.doi.org/10.1126/science.abc3593.

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Eukaryotic cells respond to stress through adaptive programs that include reversible shutdown of key cellular processes, the formation of stress granules, and a global increase in ubiquitination. The primary function of this ubiquitination is thought to be for tagging damaged or misfolded proteins for degradation. Here, working in mammalian cultured cells, we found that different stresses elicited distinct ubiquitination patterns. For heat stress, ubiquitination targeted specific proteins associated with cellular activities that are down-regulated during stress, including nucleocytoplasmic tra
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Reineke, Lucas C., Jon D. Dougherty, Philippe Pierre та Richard E. Lloyd. "Large G3BP-induced granules trigger eIF2α phosphorylation". Molecular Biology of the Cell 23, № 18 (2012): 3499–510. http://dx.doi.org/10.1091/mbc.e12-05-0385.

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Stress granules are large messenger ribonucleoprotein (mRNP) aggregates composed of translation initiation factors and mRNAs that appear when the cell encounters various stressors. Current dogma indicates that stress granules function as inert storage depots for translationally silenced mRNPs until the cell signals for renewed translation and stress granule disassembly. We used RasGAP SH3-binding protein (G3BP) overexpression to induce stress granules and study their assembly process and signaling to the translation apparatus. We found that assembly of large G3BP-induced stress granules, but n
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Dissertations / Theses on the topic "Granule Disassembly"

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Roy, Raju. "Exploring the role of low complexity protein sequence in regulating RNA granule dynamics and translation control." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5975.

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RNA granules are conserved membraneless mRNP complexes that play an important role in determining mRNA fate by affecting translation repression and mRNA decay. Processing bodies (P-bodies) harbor enzymes responsible for mRNA decay and proteins involved in modulating translation. Although many proteins have been identified to play a role in P-body assembly, a bonafide disassembly factor remains unknown. We observed that Sbp1 with the help of its RGG-motif promotes P-body disassembly in S. cerevisiae. This study provides an example of the role of low complexity sequence in RNA granule di
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Conference papers on the topic "Granule Disassembly"

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Fujime, Satoru, Shigeaki Miyamoto, Takashi Funatsu, and S. Ishiwata. "Dynamic light-scattering study on changes in mobility of chromaffin granules in actin network with its assembly and Ca2+-dependent disassembly by gelsolin." In Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, edited by Jouko E. Korppi-Tommola. SPIE, 1993. http://dx.doi.org/10.1117/12.146216.

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De Los Santos, Nancy, Robert Jones, Constantine M. Tarawneh, Arturo Fuentes, and Anthony Villarreal. "Development of Prognostic Techniques for Surface Defect Growth in Railroad Bearing Rolling Elements." In 2017 Joint Rail Conference. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/jrc2017-2262.

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Prevention of bearing failures which may lead to catastrophic derailment is a major safety concern for the railroad industry. Advances in bearing condition monitoring hold the promise of early detection of bearing defects, which will improve system reliability by permitting early replacement of failing components. However, to minimize disruption to operations while providing the maximum level of accident prevention that early detection affords, it will be necessary to understand the defect growth process and try to quantify the growth speed to permit economical, non-disruptive replacement of f
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De Los Santos, Nancy, Constantine M. Tarawneh, Robert E. Jones, and Arturo Fuentes. "Defect Prognostics Models for Spall Growth in Railroad Bearing Rolling Elements." In 2018 Joint Rail Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/jrc2018-6214.

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Prevention of railroad bearing failures, which may lead to catastrophic derailments, is a central safety concern. Early detection of railway component defects, specifically bearing spalls, will improve overall system reliability by allowing proactive maintenance cycles rather than costly reactive replacement of failing components. A bearing health monitoring system will provide timely detection of flaws. However, absent a well verified model for defect propagation, detection can only be used to trigger an immediate component replacement. The development of such a model requires that the spall
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Tarawneh, Constantine, James A. Aranda, Veronica V. Hernandez, and Claudia J. Ramirez. "An Analysis of the Efficacy of Wayside Hot-Box Detector Data." In 2018 Joint Rail Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/jrc2018-6218.

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Wayside hot-box detectors (HBDs) are devices that are currently used to monitor bearing, axle, and brake temperatures as a way of assessing railcar component health and to indicate any possible overheating or abnormal operating conditions. Conventional hot-box detectors are set to alarm whenever a bearing is operating at a temperature that is 94.4°C (170°F) above ambient, or when there is a 52.8°C (95°F) temperature difference between two bearings that share an axle. These detectors are placed adjacent to the railway and utilize an infrared sensor in order to obtain temperature measurements. B
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