Academic literature on the topic 'High performance liquid Chromatography (HPLC) and Hyphenated techniques'

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Journal articles on the topic "High performance liquid Chromatography (HPLC) and Hyphenated techniques"

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Komal, Zade* Mrunali Ahirrao Madhuri Shirsath Tufail Dana M. B. Nikam. "HPLC and its Hyphenated Techniques: Review." International Journal of Scientific Research and Technology 2, no. 5 (2025): 584–99. https://doi.org/10.5281/zenodo.15529234.

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High Performance Liquid Chromatography (HPLC) is a precise analytical technique used to separate, identify, and quantify components within complex mixtures. Developed in the late 1960s, it operates by pumping a liquid mobile phase under high pressure (50–350 bars) through a column packed with solid adsorbents like silica or polymers, which interact differently with each sample component to achieve separation. HPLC is favored in pharmaceuticals, biotechnology, environmental testing, and food industries for its speed, sensitivity, and high resolution. Hyphenated techniques in liquid chroma
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Patil, Sheetal Vikas, Anup Kishor Nalawade, Rohit Deepak Nalawade, and Shweta Prashant Ghode. "Aliskiren: analytical review for estimation in pharmaceutical formulations." World Journal of Advanced Research and Reviews 6, no. 1 (2020): 019–24. https://doi.org/10.5281/zenodo.4312514.

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Aliskiren (AL) is classified as angiotensin converting enzyme inhibitors (ACEIs) used as antihypertensive drug it acts by dilating blood vessel. These dilation of blood vessel caused due to the renin inhibition. There have been numerous methods developed so far for quantitative estimation of AL in bulk and pharmaceutical dosage form. Pharmaceutical analytical methods include UV Visible Spectrophotometry, Reversed Phase High Performance Liquid Chromatography and hyphenated techniques like Liquid chromatography- Mass Spectrometry. This review paper includes various publications focuses on advanc
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de la Fuente, M. A., and M. Juarez. "Revisión: Aplicación de las técnicas cromatográficas al estudio de triglicéridos y esteroles de la grasa de leche / Review: Application of chromatographic techniques to the study of triglycerides and sterols of milk fat." Food Science and Technology International 5, no. 2 (1999): 103–19. http://dx.doi.org/10.1177/108201329900500201.

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The latest developments in analytical techniques, mainly chromatographic, for the study of triglycer ides (TG) and sterols in milk fat are revised. Gas chromatography (GC) with packed or short capillary columns has been used to separate TG according to their carbon numbers (CN) and can be applied to the detection of foreign fats in milk fat. Combined or hyphenated chromatographic techniques are indispensable in order to identify and quantify individual molecular species of TG: thin layer chro matography (TLC) or high performance liquid chromatography (HPLC) with Ag+ as prefraction stage, follo
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Sagar, Pagade* Sahil Agrawal Prathmesh Shejal Pranjal Chougule Nilesh Chougule. "Detection Of Impurities: A Review On Advance In Impurities Detection And Characterization In Pharmaceuticals By Analytical Techniques." International Journal in Pharmaceutical Sciences 1, no. 12 (2023): 860–79. https://doi.org/10.5281/zenodo.10433875.

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The detection and characterization of impurities in pharmaceuticals are pivotal aspects of ensuring the safety, efficacy, and quality of drug products. This review provides a comprehensive overview of the analytical techniques employed in the identification and quantification of impurities at various stages of pharmaceutical development, manufacturing, and quality control. Chromatographic methods, including High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), are foundational for their ability to separate complex mixtures. Mass spectrometry (MS) and Nuclear Magnetic Reson
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Anwar, Shumaila, Akbar Khan, Mariyum Jamal, and Muhammad Zain Siddiqui. "Review on the modern analytical advancements in impurities testing." Advances in Analytic Science 6, no. 1 (2025): 3159. https://doi.org/10.54517/aas3159.

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<p>Impurities in active pharmaceutical ingredients (APIs) and pharmaceutical drug products (DPs) lead to broader antithetical effects related to drug safety, efficacy, and regulatory compliance. This review discusses organic, elemental, and inorganic impurities, and residual solvents and stresses their impact on the quality of APIs and pharmaceutical DPs regarding patient safety. It endorses immensely developed contemporary analytical techniques like High-performance Liquid Chromatography (HPLC), and Gas Chromatography (GC), for organic impurities and discusses their hyphenated Mass Spec
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Adhikari, Rudra Prasad, Samima Nasrin Rahman, Suman Lamichhane, and Aditya Bora. "Overview of Analytical Methods for the Determination of H2 Receptor Blockers: A Review." Journal of Drug Delivery and Therapeutics 13, no. 10 (2023): 137–44. http://dx.doi.org/10.22270/jddt.v13i10.6245.

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Overview of various analytical methods for estimating anti-histaminic (H2 Receptor blockers) drugs, particularly for determining their concentration percentage (assay) by analytical methods developed on analytical instruments i.e., UV visible Spectrophotometer, High-Performance Liquid Chromatography, and Hyphenated techniques. The review includes a literature survey of H2 receptor blocker drugs namely cimetidine (Tagamet), ranitidine (Zantac), famotidine (Pepcid, Pepcid AC), and nizatidine (Axid). The examined literature survey addressed chromatographic (HPLC) and UV visible spectrophotometric
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Madhuri, R. Shirsath Sonali A. Waghmare And Pradyumna P. Ige. "A Review On Recent Advances In Development Of RP-HPLC Method." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2674–82. https://doi.org/10.5281/zenodo.13242715.

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The development and validation of analytical methods play crucial roles in drug discovery, drug development, and the production of different pharmaceutical formulations. A straightforward, accurate, exact, and targeted RP-HPLC approach was created to determine the drug in pharmaceuticals. Because of its greater selectivity and flexibility for hydrophobic substances, reverse-phase liquid chromatography (RP-HPLC) is the separation method of choice for high-performance liquid chromatography (HPLC). The development of RP HPLC methods has advanced significantly in recent years, with new stationary
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G, Preethi, Siva Prasad M., Prachet P., and Rama Rao N. "Analytical Methods for the Estimation of Levothyroxine and Levothyroxine Sodium in Pharmaceutical Formulations – A Review." International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 16, no. 6 (2023): 7104–13. http://dx.doi.org/10.37285/ijpsn.2023.16.6.10.

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Levothyroxine (L-T4) is used in the treatment of hypothyroidism and is a synthetic levoisomer of the thyroid hormone thyroxine produced by the thyroid gland. Levothyroxine sodium (L-T4S) salt has a high clinical utility. There are numerous studies that have been published that describe analytical methods for the estimation of L-T4 and L-T4S in pharmaceutical formulations and also in various biological matrices like blood, plasma, serum, and urine. This review article focused on analytical methods for estimating L-T4 and L-T4S in pharmaceutical formulations such as Thin Layer Chromatography (TL
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Mandal, Badal Kumar, and Yong-Chien Ling. "Analysis of Chlorophylls/Chlorophyllins in Food Products Using HPLC and HPLC-MS Methods." Molecules 28, no. 10 (2023): 4012. http://dx.doi.org/10.3390/molecules28104012.

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Of the different quality parameters of any food commodity or beverage, color is the most important, attractive and choice-affecting sensory factor to consumers and customers. Nowadays, food industries are interested in making the appearance of their food products attractive and interesting in order to appeal to consumers/customers. Natural green colorants have been accepted universally due to their natural appeal as well as their nontoxic nature to consumers. In addition, several food safety issues mean that natural green colorants are preferable to synthetic food colorants, which are mostly u
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Rutuja, V. Pagare, and Phadtare Dipti. "An Overview of The Hyphenated Separation Technology and its Application in the Pharmacy." Int. J. in Pharm. Sci. 1, no. 1 (2021): 1–11. https://doi.org/10.5281/zenodo.5483833.

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Traditional analytical approaches, including high performance liquid chromatography (HPLC), gas chromatography (GC), and ultraviolet (UV) detection, cannot effectively address the emerging problems of species specificity and sensitivity analysis. A modern analytical technique called transferring is depend upon the traditional use of specific detection of molecules or elements in electrophoresis or chromatography. Currently, the most common methods for the estimation of trace elements include a combination of separation methods and more sensitive detection methods. Previously, this binding meth
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Dissertations / Theses on the topic "High performance liquid Chromatography (HPLC) and Hyphenated techniques"

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Wang, Tongwen. "Hyphenated HPLC-MS technique for analysis of compositional monosaccharides of transgenic corn glycoprotein and characterization of degradation products of diazinon, fonofos and aldicarb in various oxidation systems." Diss., Rolla, Mo. : University of Missouri-Rolla, 2007. http://scholarsmine.mst.edu/thesis/pdf/WangTongwen_09007dcc804e975c.pdf.

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Thesis (Ph. D.)--University of Missouri--Rolla, 2007.<br>Vita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed April 23, 2008) Includes bibliographical references.
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Weddle, Carolyn A. "Optimization of HPLC techniques for separation of oxidized phosphatidylcholines." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1319835.

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In cellular studies of patients with lipid related disorders such as mammary cancers, leukemia, and artheroscierosis, separation of molecular species of oxidized phosphatidylcholine (PC) can be an important assistance in research or diagnosis. Goals of this project were to optimize separation of oxidized and unoxidized PC molecular species in a single HPLC chromatogram followed by in line identification of hydroperoxides. Oxidized egg PC's were produced using UV light exposure in air. Separations were performed on an Ultrasphere ODS column and an Asahipak ODPVA column using a Waters 2695 syste
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Nestola, Marco [Verfasser], and Torsten Claus [Akademischer Betreuer] Schmidt. "Multidimensional high-performance liquid chromatography–gas chromatography (HPLC-GC) hyphenation techniques for food analysis in routine environments / Marco Nestola ; Betreuer: Torsten Claus Schmidt." Duisburg, 2016. http://d-nb.info/1115654659/34.

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Dhoot, Gaurav. "Estimation of eugenol diffusion coefficient in LLDPE using FTIR-ATR flow cell and HPLC techniques." Diss., Connect to online resource - MSU authorized users, 2008.

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Kamble, Ujjwala Kerba. "Use of liquid chromatography for assay of flavonoids as key constituents and antibiotics as trace elements in propolis : investigation into the application of a range of liquid chromatography techniques for the analysis of flavonoids and antibiotics in propolis, and extraction studies of flavonoids in propolis." Thesis, University of Bradford, 2016. http://hdl.handle.net/10454/14564.

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Propolis is an approved food additive containing flavonoids as a major active constituent. Variability has been found in the composition of propolis in distinctive regions and it was noticed that there are limitations in the analysis of propolis. In this study, the identification of ten flavonoids and residual antibiotics in propolis was investigated by using several liquid chromatography techniques, including reversed-phase high-performance liquid chromatography (RP-HPLC), microemulsion LC (MELC) and ultra-performance LC (UPLC). The ten flavonoids that were selected for this research include
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Howarth, John Eric. "Alpha Thalassaemia; early detection and diagnosis : the application of high performance liquid chromatography (HPLC) and molecular biology techniques for the detection and characterisation of Alpha Thalassaemia." Thesis, University of Salford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313933.

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Mnatsakanyan, Mariam. "Natural antioxidants and high performance liquid chromatography (HPLC) hyphenated screening techniques." Thesis, 2010. http://handle.uws.edu.au:8081/1959.7/496789.

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Reactive intermediates in the oxidative processes are among the major sources of primary catalysts that initiate oxidation in vivo and in vitro; a process that is one of the major causes of food quality deterioration and generation of chronic diseases. Antioxidants, both natural and synthetic, have been widely used at legal limits in food, medical and personal care industries. However, recently, the use of synthetic antioxidants has been severely restricted because of the evidence that suggests they may be harmful to human health. As such, the importance of screening naturally occurring altern
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Books on the topic "High performance liquid Chromatography (HPLC) and Hyphenated techniques"

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Drucker, D. B. Microbiological applications of high-performance liquid chromatography. Cambridge University Press, 1987.

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White, Peter Cyril. HPLC multiwavelength UV/visible detection and separation techniques for the characterisation of acidic dyes encountered in forensic science. [s.n.], 1991.

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Hearn, Milton T. W. Hplc of Proteins Peptides and Polynucleotides: Current Topics and Applications (Analytical Techniques in Clinical Chemistry and Laboratory Medicine). Vch Pub, 1991.

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Sherma, Joseph, and Tomasz Tuzimski. High Performance Liquid Chromatography in Pesticide Residue Analysis. Taylor & Francis Group, 2015.

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Sherma, Joseph, and Tomasz Tuzimski. High Performance Liquid Chromatography in Pesticide Residue Analysis. Taylor & Francis Group, 2015.

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High Performance Liquid Chromatography in Pesticide Residue Analysis. Taylor & Francis Group, 2015.

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Book chapters on the topic "High performance liquid Chromatography (HPLC) and Hyphenated techniques"

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Potey, Lata, Ashwini Armarkar, Shilpa Chapekar, Yogini Shete, Pooja Birade, and Suchita Waghmare. "APPLICATIONS OF HYPHENATED TECHNIQUES IN PHARMACEUTICAL SCIENCES." In Futuristic Trends in Chemical Material Sciences & Nano Technology Volume 3 Book 18. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bdcs18ch19.

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The hyphenation term was introduced by Thomas Hirschfield in 1980. Hyphenated techniques are exponentially to deal with various complicated challenges faced by analyst in analytical chemistry. It is the fusion of chromatographic methods and spectrometric or spectroscopic methods. Chromatographic methods are separation methods which separate component in the complex mixture like gas chromatography (GC), liquid chromatography (LC), high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Spectroscopy use to elucidate the structure, molecular mass of compounds like UV, IR
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Sewell, Peter A. "High-performance liquid chromatography." In Lipid Analysis. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199630981.003.0005.

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Abstract High-performance liquid chromatography (HPLC) is, in many areas, the main technique used for analysis. However, in the area of lipid analysis its use is still relatively uncommon, and thin-layer chromatography (TLC) and gas chromatography (GC) are the more commonly used techniques. Both of these techniques have advantages and disadvantages compared to HPLC: TLC is a very simple and cheap technique but it is a relatively inefficient process and is difficult to quantitate; GLC (gas-liquid chromatography) is easier to use and the equipment is cheaper than HPLC, but samples must be capabl
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Desai, Saloni S., Sejal M. Khuman, and Jeshika B. Patel. "HIGH PERFORMANCE LIQUID CHROMATOGRAPHY." In Emerging Pharmaceutical Sciences: Today and Tomorrow. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/nbennurch334.

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One type of column chromatography that is commonly used in biochemistry and analysis for the purpose of separating and quantify active compounds is called High Performance Liquid Chromatography (HPLC). HPLC is a frequently employed separation technique that is utilized to determine, separate, and measure the medication. In addition to a variety of various human and animal study, the development and validation of highperformance liquid chromatography techniques play critical roles in innovative drug identification, development, and production. The several steps needed in creating and verifying
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Lozano-Sánchez, Jesús, Isabel Borrás-Linares, Agnes Sass-Kiss, and Antonio Segura-Carretero. "Chromatographic Technique: High-Performance Liquid Chromatography (HPLC)." In Modern Techniques for Food Authentication. Elsevier, 2018. http://dx.doi.org/10.1016/b978-0-12-814264-6.00013-x.

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Gazdag, Mária. "2.7 High performance liquid chromatography (HPLC) and related techniques." In Identification and Determination of Impurities in Drugs. Elsevier, 2000. http://dx.doi.org/10.1016/s1464-3456(00)80014-9.

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Aranyi, Antal. "2.7 High performance liquid chromatography (HPLC) and related techniques." In Identification and Determination of Impurities in Drugs. Elsevier, 2000. http://dx.doi.org/10.1016/s1464-3456(00)80015-0.

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Görög, Sándor. "2.7 High performance liquid chromatography (HPLC) and related techniques." In Identification and Determination of Impurities in Drugs. Elsevier, 2000. http://dx.doi.org/10.1016/s1464-3456(00)80016-2.

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Tollsten, Lars. "2.7 High performance liquid chromatography (HPLC) and related techniques." In Identification and Determination of Impurities in Drugs. Elsevier, 2000. http://dx.doi.org/10.1016/s1464-3456(00)80017-4.

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Bélanger, Jacqueline M. R., J. R. Jocelyn Paré, and Michel Sigouin. "Chapter 2 High performance liquid chromatography (HPLC): Principles and applications." In Techniques and Instrumentation in Analytical Chemistry. Elsevier, 1997. http://dx.doi.org/10.1016/s0167-9244(97)80011-x.

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Tutar, Esen. "High Performance Liquid Chromatography (HPLC) – Theoretical and Practical Aspects." In Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications- Part II. BENTHAM SCIENCE PUBLISHERS, 2020. http://dx.doi.org/10.2174/9789811464867120010005.

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Conference papers on the topic "High performance liquid Chromatography (HPLC) and Hyphenated techniques"

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Wang, Xuejun, Andrew (Yanjiao) Xie, James Michels, and Donald Johnson. "Corrosion Inhibition Mechanism Study of a Novel Yellow Metal Corrosion Inhibitor." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-12847.

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Abstract The corrosion inhibition mechanism of a new yellow metal corrosion inhibitor was studied using multiple characterization techniques including ellipsometry, white light interferometry (WLI), grazing incidence reflection absorption Fourier transform infrared (GIRA-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS) and high-performance liquid chromatography (HPLC). Compared to traditional azole molecules, the novel yellow metal corrosion inhibitor develops a stimuli-responsive film, which would reduce the impact of high levels of halo
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Barker, Richard, Richard C. Woollam, and William H. Durnie. "Implications of Physical and Chemical Environment on the Distribution of Corrosion Inhibitor Surfactant Components and Mixtures." In CONFERENCE 2025. AMPP, 2025. https://doi.org/10.5006/c2025-00436.

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Abstract Corrosion inhibitors are a proven cost-effective method for internal corrosion protection of carbon steel piping in the presence of CO2-containing brines. The appropriate selection, deployment and performance monitoring are all essential elements ensuring successful application. However, despite many years of use within the oil and gas industry, a clear understanding of the impact of the physical chemistry of surfactants and surfactant mixtures still eludes the industry, and chemical law is often replaced with oilfield lore. In this work, the effect of brine salinity (0.1, 1 and 10 wt
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Guo, Zhongxian, Qiantao Cai, and Zhaoguang Yang. "Determination of Water-Soluble Organophosphorus Herbicides by Ion Chromatography With Inductively Coupled Plasma Mass Spectrometry Detection." In 1st Water Quality, Drought, Human Health and Engineering Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/water2006-20024.

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There is a high risk for human exposure to organophosphorus pesticides through contaminated drinking water. Glyphosate, glufosinate, fosamine and ethephon are among the water-soluble herbicides used currently. Sensitive and rapid analytical methodologies are critical for evaluating their residuals in a broad variety of samples, including environmental waters. However, challenges arise from the inherent chemical properties of the herbicides: strong polarity, high solubility in water, insolubility in organic solvent (except ethephon), absence of chromophore or fluorophore in their molecular stru
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Blebea, Nicoleta Mirela, and Simona Negreș. "METHODS FOR QUANTIFICATION OF THE MAIN CANNABINOIDS IN CBD OIL." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/13.

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Cannabidiol (CBD) is an alkaloid present in Cannabis sativa, together with tetrahydrocannabinol (THC) and more than 120 other substances belonging to a group of compounds named cannabinoids. Due to the continuous increased usage of CBD oils, it became necessary to be developed efficient methods for the identification of its compounds and especially for the characterization of the cannabinoids from the commercial specimens. Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat c
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Marquardt, Drew, and Stuart Castillo. "Determination of oxidation stability and shelf life of cannabis formulations." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/togo4822.

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The characterization of cannabis extract in an industrial setting is required for both quality assurance (QA), quality control (QC) and antioxidant research and development (R&amp;D) purposes. More importantly, having a rapid and straightforward streamlined process is essential for the daily operational success of any R&amp;D department. Using physical techniques such as polarimetry, refractometry, and densitometry, the physical properties of three cannabis extract compositions in carrier oils were characterized and examined: pure Tetrahydrocannabinol (THC), Cannabidiol (CBD), and a THC/CBD mi
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Erazo, Maria P., William Singer, Nick Lord, Maria L. Rosso, and Bo Zhang. "Development of a Near-infrared Spectroscopy Calibration Model to Predict Methionine Content in Whole Soybeans." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/zusl9413.

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Soybean is an important source of high-quality protein for humans and livestock. However, the content of methionine, a sulfur-containing essential amino acid, is deficient and inadequate for consumer needs. Amino acid evaluation can be performed by conventional wet chemical techniques like high-performance liquid chromatography (HPLC). To ease out the need for complex laboratory infrastructure and highly skilled experts often required by these procedures, HPLC datasets can be paired with data obtained from Near-infrared (NIR) reflectance spectroscopy. Through this approach, spectral data can b
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Tyukodi, Levente. "Comparison of Predictive Values with Experimental Results for Cyclic C5-Curcuminoids." In 8th World Conference on Chemistry and Chemical Engineering and 8th World Conference on Advanced Materials, Nanoscience and Nanotechnology. Eurasia Conferences, 2025. https://doi.org/10.62422/978-81-981865-7-7-010.

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Curcuminoids, both C7- and C5-derivatives, are natural bioactive compounds with diverse pharmacological properties. Structural modifications, such as the incorporation of a cyclic system into the carbon chain, can significantly alter their physicochemical characteristics, including lipophilicity and solubility as key factors influencing biological activity. This study compares the predicted lipophilicity and solubility of C5 cyclic curcuminoids with experimental data. Lipophilicity estimation is a critical parameter in drug development, typically assessed through high-throughput screening (HTS
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Herman, G., C. Bittner, N. Watts, and A. Mishra. "Promoting Field Success in Conventional EOR: Harmonizing Field-Scale Formulation for Commercial Volume Deployment." In SPE Conference at Oman Petroleum & Energy Show. SPE, 2025. https://doi.org/10.2118/225063-ms.

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Abstract Enhanced Oil Recovery (EOR) surfactant formulations encounter significant challenges when scaling from laboratory research to full-field commercial deployment. These challenges arise due to variations in surfactant molecular structures, inconsistencies in raw materials, and complexities associated with global supply chain harmonization. This study presents a structured approach to optimizing and standardizing surfactant formulations through rigorous laboratory evaluations, advanced analytical techniques—particularly High-Performance Liquid Chromatography (HPLC)—and robust quality cont
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Tschanner, Julius, Jinchang Ren, Frances Jack, Stephen Marshall, and Huimin Zhao. "Employing NIR-SWIR hyperspectral imaging to predict the smokiness of scotch whisky." In OCM 2017 - 3rd International Conference on Optical Characterization of Materials. KIT Scientific Publishing, 2017. http://dx.doi.org/10.58895/ksp/1000063696-1.

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Scotch Whisky makes a significant contribution to the UK’s food and drinks export. The flavour of this high quality spirit is derived naturally from the whisky making process, with smoky aromas being a key character of certain Scotch whiskies. The level of smokiness is determined by the amount of phenolic compounds in the spirit. Phenols are introduced by exposing the barley malt to peat smoke during the kilning process. The current techniques to determine the levels of phenols, such as High Performance Liquid Chromatography (HPLC), are time consuming as they require distillation of the malt p
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