Academic literature on the topic 'Illumina Mi-Seq'

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Journal articles on the topic "Illumina Mi-Seq"

1

Urumbil, Sithara K., and M. Anil Kumar. "Diversity Analysis of Endophytic Bacterial Microflora in Emilia sonchifolia (Linn.) DC on Illumina Mi Seq Platforms." Journal of Pure and Applied Microbiology 14, no. 1 (2020): 679–87. http://dx.doi.org/10.22207/jpam.14.1.70.

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Haenel, Quiterie, Oleksandr Holovachov, Ulf Jondelius, Per Sundberg, and Sarah Bourlat. "NGS-based biodiversity and community structure analysis of meiofaunal eukaryotes in shell sand from Hållö island, Smögen, and soft mud from Gullmarn Fjord, Sweden." Biodiversity Data Journal 5 (June 8, 2017): e12731. https://doi.org/10.3897/BDJ.5.e12731.

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Aim: The aim of this study was to assess the biodiversity and community structure of Swedish meiofaunal eukaryotes using metabarcoding. To validate the reliability of the metabarcoding approach, we compare the taxonomic resolution obtained using the mitochondrial cytochrome oxidase 1 (COI) 'mini-barcode' and nuclear 18S small ribosomal subunit (18S) V1-V2 region, with traditional morphology-based identification of Xenacoelomorpha and Nematoda. Location: 30 samples were analysed from two ecologically distinct locations along the west coast of Sweden. 18 replicate samples of coarse shell sand we
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Atherton, Sarah, and Ulf Jondelius. "Biodiversity between sand grains: Meiofauna composition across southern and western Sweden assessed by metabarcoding." Biodiversity Data Journal 8 (April 27, 2020): e51813. https://doi.org/10.3897/BDJ.8.e51813.

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The meiofauna is an important part of the marine ecosystem, but its composition and distribution patterns are relatively unexplored. Here we assessed the biodiversity and community structure of meiofauna from five locations on the Swedish western and southern coasts using a high-throughput DNA sequencing (metabarcoding) approach. The mitochondrial cytochrome oxidase 1 (COI) mini-barcode and nuclear 18S small ribosomal subunit (18S) V1-V2 region were amplified and sequenced using Illumina MiSeq technology. Our analyses revealed a higher number of species than previously found in other areas: th
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Zhang, Fengling, Xingjia Xiang, Yuanqiu Dong, Shaofei Yan, Yunwei Song, and Lizhi Zhou. "Significant Differences in the Gut Bacterial Communities of Hooded Crane (Grus monacha) in Different Seasons at a Stopover Site on the Flyway." Animals 10, no. 4 (2020): 701. http://dx.doi.org/10.3390/ani10040701.

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Intestinal bacterial communities form an integral component of the organism. Many factors influence gut bacterial community composition and diversity, including diet, environment and seasonality. During seasonal migration, birds use many habitats and food resources, which may influence their intestinal bacterial community structure. Hooded crane (Grus monacha) is a migrant waterbird that traverses long distances and occupies varied habitats. In this study, we investigated the diversity and differences in intestinal bacterial communities of hooded cranes over the migratory seasons. Fecal sample
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Nadifah, Fitri, Wayan Tunas Artama, Budi Setiadi Daryono, and Endah Retnaningrum. "Characterization of the urogenital microbiome in patients with urinary tract infections." Indonesian Journal of Biotechnology 27, no. 3 (2022): 142. http://dx.doi.org/10.22146/ijbiotech.69212.

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Standard microbiological culture techniques can only identify a fraction of the urogenital microbiome. Meanwhile, identifying and characterizing infectious microorganisms are very important for the success of diagnosis and treatments, especially for Urinary Tract Infection (UTI) patients. This study aimed to characterize the urogenital microbiome of UTI patients using 16S rRNA gene sequencing. We sequenced two pooled DNA samples from voided urine of UTI patients (21 females and 13 males). To determine the structure and composition of taxa in the samples, 16S rRNA gene sequencing was performed
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Kushkevych, Ivan, Monika Vítězová, Tomáš Vítěz, and Milan Bartoš. "Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms." Open Life Sciences 12, no. 1 (2017): 82–91. http://dx.doi.org/10.1515/biol-2017-0009.

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AbstractThe production of high-quality methane depends on many factors, including temperature, pH, substrate, composition and relationship of the microorganisms. The qualitative and quantitative composition of methanogenic and sulfate-reducing microorganisms and their relationship in the experimental bioreactors has never been studied. The aim of this research was to characterize, for the first time, the diversity of the methanogenic microorganisms and sulfate-reducing bacteria, and study their relationship and biogas production in experimental bioreactors. Amplification of 16S rRNA gene fragm
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Chitwood, J. L., V. R. Burruel, S. A. Meyers, and P. J. Ross. "131 RNA-Seq TRANSCRIPTOME PROFILING OF INDIVIDUAL RHESUS MACAQUE OOCYTES AND PRE-IMPLANTATION EMBRYOS." Reproduction, Fertility and Development 26, no. 1 (2014): 179. http://dx.doi.org/10.1071/rdv26n1ab131.

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The objective of this study was to perform whole transcriptome sequencing (RNA-Seq) on individual rhesus macaque oocytes and intracytoplasmic sperm injection-derived embryos. Nine developmental stages were assayed (n = 2–3 per stage): germinal vesicle (GV), meiosis I (MI), and meiosis II (MII) oocytes and pronuclear (PN), 2-cell (2C), 4-cell (4C), 8-cell (8C), morula (MO), and blastocyst (BL) stage embryos. Rhesus females were superovulated with hrFSH and oocytes collected by follicular aspiration of ovaries recovered at necropsy 35 h after hCG treatment. Based on GV and polar body (PB) presen
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Jones, James M. C., Elizabeth A. Webb, Michael D. J. Lynch, Trevor C. Charles, Pedro M. Antunes, and Frédérique C. Guinel. "Does a carbonatite deposit influence its surrounding ecosystem?" FACETS 4, no. 1 (2019): 389–406. http://dx.doi.org/10.1139/facets-2018-0029.

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Carbonatites are unusual alkaline rocks with diverse compositions. Although previous work has characterized the effects these rocks have on soils and plants, little is known about their impacts on local ecosystems. Using a deposit within the Great Lakes–St. Lawrence forest in northern Ontario, Canada, we investigated the effect of a carbonatite on soil chemistry and on the structure of plant and soil microbial communities. This was done using a vegetation survey conducted above and around the deposit, with corresponding soil samples collected for determining soil nutrient composition and for a
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Baptista Neto, José Antônio, Christine Gaylarde, Iwona Beech, Bernard J. Smith, and John J. McAlister. "Degradação de gnaisse e granito em fachadas de edifícios históricos no centro do Rio de Janeiro." Sistemas & Gestão 15, no. 1 (2020): 80–90. http://dx.doi.org/10.20985/1980-5160.2020.v15n1.1621.

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A maioria do patrimônio cultural construído na cidade do Rio de Janeiro, Brasil, é feito de gnaisse ou granito, e a exposição das fachadas ao ambiente poluído leva a uma forte degradação. Para entender estes processos meteorológicos, foram estudados cinco edifícios históricos na cidade. Estes exibiram manchas de ferro, desintegração granular, bolhas, fraturas incipientes e escamação de contornos e desenvolvimento de crosta negra. As amostras coletadas nestes edifícios foram examinadas na tentativa de compreender os mecanismos da meteorologia de superfície. Amostras de rochas foram coletadas em
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Pellegrini, Marika, Daniela M. Spera, Claudia Ercole, and Maddalena del Gallo. "Allium cepa L. Inoculation with a Consortium of Plant Growth-Promoting Bacteria: Effects on Plant Growth and Development and Soil Fertility Status and Microbial Community." Proceedings 66, no. 1 (2021): 20. http://dx.doi.org/10.3390/proceedings2020066020.

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The present work was aimed at investigating the effects of a four strains consortium—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, and Burkholderia ambifaria—on crops of Allium cepa L. and its soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated seeds and control ones (treated with autoclaved inoculum) were sown in open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-S
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