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1

Bagheri, fatemeh, Saeed Mirdamadi, Mahta Mirzaei, and Maliheh Safavi. "Risk Assessment of Isolated Lactobacill from Traditional Iranian Dairy Products." Food Science and Technology 17, no. 104 (2020): 65–78. http://dx.doi.org/10.52547/fsct.17.104.65.

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2

Kataeva, L. V., A. A. Vakarina, O. N. Kolotova, et al. "Age features of species diversity lactobacill of large intestine in residents of Tyumen." Journal of microbiology epidemiology immunobiology, no. 3 (August 22, 2019): 10–15. http://dx.doi.org/10.36233/0372-9311-2019-3-10-15.

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3

Chen, Chunfei, Leilei Yu, Fengwei Tian, Jianxin Zhao, and Qixiao Zhai. "Identification of Novel Bile Salt-Tolerant Genes in Lactobacillus Using Comparative Genomics and Its Application in the Rapid Screening of Tolerant Strains." Microorganisms 10, no. 12 (2022): 2371. http://dx.doi.org/10.3390/microorganisms10122371.

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Under bile salt treatment, strains display significant differences in their tolerance ability, suggesting the existence of diverse resistance mechanisms in Lactobacillus; however, the genes involved in this protective process are not fully understood. In this study, novel target genes associated with bile salt tolerance in Lactobacillus were identified using comparative genomics for PCR detection and the rapid screening of tolerant strains. The bile salt tolerance of 107 lactobacilli isolated from different origins was assessed, and 26 strains with comparatively large differences were selected
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4

GOMES, A. M. P., M. G. M. TEIXEIRA, and F. X. MALCATA. "VIABILITY OF BIFIDOBACTERIUM LA CTIS AND LACTOBACILL US ACIDOPHILUS IN MILK: SODIUM CHLORIDE CONCENTRATION AND STORAGE TEMPERATURE." Journal of Food Processing and Preservation 22, no. 3 (1998): 221–40. http://dx.doi.org/10.1111/j.1745-4549.1998.tb00347.x.

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5

Bilkova, A., H. Kinova Sepova, M. Bukovsky, and L. Bezakova. "Antibacterial potential of lactobacilli isolated from a lamb." Veterinární Medicína 56, No. 7 (2011): 319–24. http://dx.doi.org/10.17221/1583-vetmed.

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The antimicrobial properties of three potential probiotic strains of lactobacilli isolated from a lamb (Lactobacillus murinus C, Lactobacillus mucosae D and Lactobacillus reuteri E) were studied using the streak line method and the agar well diffusion assay. The probiotic lactobacilli strains Lactobacillus rhamnosus ATCC 53103, Lactobacillus reuteri ATCC 55730, Lactobacillus reuteri ATCC 55845 and Lactobacillus plantarum DSM 9843 were used for comparison. Using the streak line method the inhibitory activity of lactobacilli products towards ten Gram-positive and Gram-negative potential pathogen
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6

Pravdivtseva, M. I., L. V. Karpunina, and M. D. Smetanina. "Effect of Exopolysaccharides of Lactobacill on the Microflora of Large Intestine in Female Rats at Various Kinds of Stress." Izvestiya of Saratov University. Chemistry. Biology. Ecology 11, no. 2 (2011): 89–94. http://dx.doi.org/10.18500/1816-9775-2011-11-2-89-94.

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The effect of exopolysaccharides of lactic acid bacteria – laksarans 1596, 1936 and Z on the microflora of large intestine in female rats at various kinds of stress. It was shown that laksarans able to stimulate the growth of some lactic acid bacteria and inhibit the growth of some opportunistic microflora in the large intestine with the negative impacts caused by immobilization, cold and ethanol stress, acting as prebiotics.
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7

Ghazaei, Ciamak. "Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria." Research in Molecular Medicine 8, no. 4 (2020): 189–200. http://dx.doi.org/10.32598/rmm.8.4.4.

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Background: Probiotics are “live microbial cells” that are beneficial for human and animal health. Lactobacilli are such a diverse group of bacteria with similar metabolic and physiological characteristics, and constitute important and beneficial gut microflora. During carbohydrate fermentation, lactobacilli produce lactic acid as an end product in metabolism. Hence, lactobacilli have high significance to be used as probiotics in the food industry, because of their acidifying properties. Also, lactobacilli are considered “safe”, owing to their ubiquitous presence in the food. Many researchers
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8

Bobcek, B., Ľ. Gajdosová, M. Kacániová, J. Lejková, L. Hleba, and V. Kmeť. "Antibacterial susceptibility of lactobacilli isolated from pork meat of conventional breeding." Biotehnologija u stocarstvu 27, no. 3 (2011): 799–807. http://dx.doi.org/10.2298/bah1103799b.

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The aim of this study was monitoring of antibiotic resistance of Enterobacteriaceae genera isolated from musculus longissimus at thoracis and musculus semimembranosus of pork. The pigs was feeding in two groups (control and experimental wit selenium application). For the antibiotic susceptibility testing disk diffusion method was used. Lactobacillus species were tested against two antibiotics: ampiciline, tetracycline. For the detection and identification of lactobacilli we used MRS and Rogose agar. For identification of lactobacilli MALDI-TOF-MS were used. The higher resistance of isolated ba
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9

MURAD, H. A., R. I. REFAEA та E. M. ALY. "Utilization of UF-Permeate for Production of β-galactosidase by Lactic Acid Bacteria". Polish Journal of Microbiology 60, № 2 (2011): 139–44. http://dx.doi.org/10.33073/pjm-2011-019.

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Four lactobacilli strains (Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacilus casei and Lactobacillus reuteri) were grown in MRS broth and three lactococci strains (Streptococcus thermophilus, Lactococcus lactis subsp. Lactis and Lactococcus lactis subsp. lactis biovar. diacetilactis) were grown in M17 broth. L. reuteri and S. thermophilus were chosen on the basis of the best mean beta-galactosidase activity of 10.44 and 10.01 U/ml respectively, for further studies on permeate-based medium. The maximum production of beta-galactosidase by L. reuteri was achieved at lactose concen
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10

Stojanov, Spase, Tina Vida Plavec, Julijana Kristl, Špela Zupančič, and Aleš Berlec. "Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers." International Journal of Molecular Sciences 22, no. 24 (2021): 13631. http://dx.doi.org/10.3390/ijms222413631.

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Lactobacilli are a promising natural tool against vaginal dysbiosis and infections. However, new local delivery systems and additional knowledge about their distribution and mechanism of action would contribute to the development of effective medicine. This will be facilitated by the introduction of the techniques for effective, inexpensive, and real-time tracking of these probiotics following their release. Here, we engineered three model vaginal lactobacilli (Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacill
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11

Dong, Jie, Yun Sheng Jiang, Hai Yu, Wen Juan Zhang, and Yu Feng Liu. "Study on Lactobacillus Isolation from Rabbit Meat Sausage and its Fermentation Property." Applied Mechanics and Materials 140 (November 2011): 431–40. http://dx.doi.org/10.4028/www.scientific.net/amm.140.431.

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Three lactobacilli (L26, L34 , L106) isolated from naturally fermented rabbit sausage in a traditional way are identified as lactobacillus plantarum, lactobacillus sake and lactobacillus fructosus. Further study of its fermentation property shows that three lactobacilli are effective in acid production, alkali resistant, nitrite resistant and bacteriostatic, and are antagonisticaction free, which can be used as combined rabbit meat starter.
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12

CHANG, M. H., and T. C. CHEN. "Reduction of Campylobacter jejuni in a Simulated Chicken Digestive Tract by Lactobacilli Cultures." Journal of Food Protection 63, no. 11 (2000): 1594–97. http://dx.doi.org/10.4315/0362-028x-63.11.1594.

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Studies were conducted to investigate the impact of a selected lactobacilli mixed culture on Campylobacter jejuni in simulated chicken digestive tract models. Veronal buffer solutions corresponding to the pH of successive segments of the chicken digestive tract were prepared. The lactobacilli mixtures were prepared by mixing four fresh lactobacilli cultures, including Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus crispatus, and Lactobacillus brevis. The C. jejuni and lactobacilli mixture were mixed with sterile poultry feed, and the previously prepared veronal buffer soluti
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13

Výrostková, Jana, Ivana Regecová, Mariana Kováčová, Slavomír Marcinčák, Eva Dudriková, and Jana Maľová. "Antimicrobial Resistance of Lactobacillus johnsonii and Lactobacillus zeae in Raw Milk." Processes 8, no. 12 (2020): 1627. http://dx.doi.org/10.3390/pr8121627.

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Lactobacillus johnsonii and Lactobacillus zeae are among the lactobacilli with probiotic properties, which occur in sour milk products, cheeses, and to a lesser extent in raw milk. Recently, resistant strains have been detected in various species of lactobacilli. The aim of the study was to determine the incidence of resistant Lactobacillus johnsonii and Lactobacillus zeae strains in various types of raw milk. A total of 245 isolates were identified by matrix-assisted laser desorption/ionization mass spectrometry and polymerase chain reaction methods as Lactobacillus sp., of which 23 isolates
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14

Guizani, N., and K. Al-Ramadani. "Microflora and Physical-Chemical Characteristics of Omani Laban." Journal of Agricultural and Marine Sciences [JAMS] 4, no. 2 (1999): 61. http://dx.doi.org/10.24200/jams.vol4iss2pp61-64.

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Fifteen samples of Laban made at home in three Omani regions were subjected to physical-chemical and microbiological analysis. Laban had an average titratable acidity, pH, fat, protein and total solids of 1.12%, 3.98, 1.I2%, 2. 11% and 6.29%, respectively. The microbial flora of traditional Omani laban was found to be predominantly mesophilic lactococci. and homofemenentative lactobacili. The mean Lactococci and lactobacilli counts were 1.3 x 10 8 and 2.4 x 10 6/ml respectively. The main microbial types involved in the manufacture of Omani laban were Lactoeoccus lactis ssp lactis. Lacrococcus
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15

Karlsson, M., and J. Jass. "Lactobacilli differently regulate expression and secretion of CXCL8 in urothelial cells." Beneficial Microbes 3, no. 3 (2012): 195–203. http://dx.doi.org/10.3920/bm2012.0011.

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Modulation of the immune response is an established feature of certain lactobacilli. CXCL8 is an inflammatory chemokine released by the urinary tract mucosa after contact with uropathogenic Escherichia coli during urinary tract infection and is crucial for proper infiltration of immune cells. Nevertheless, persistently high levels of CXCL8 are associated with pathogenicity and malignancy. In this study, we tested twelve Lactobacillus strains for their ability to influence CXCL8 release from urothelial cells. We evaluated how strains from different Lactobacillus species could regulate CXCL8 in
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16

Villa, Andrés F., Adelina P. Meléndez, Juan E. Carulla, Martha L. Pabón, and Edgar A. Cárdenas. "Estudio microbiológico y calidad nutricional del ensilaje de maíz en dos ecorregiones de Colombia." Revista Colombiana de Ciencias Pecuarias 23, no. 1 (2013): 65–77. http://dx.doi.org/10.17533/udea.rccp.324531.

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Resumen El presente estudio buscó establecer los cambios en la concentración inicial de los géneros de bacterias acido lácticas (BAL) en dos variedades de maíz utilizado para ensilar en clima cálido y frío respectivamente. Se realizaron microsilos de 1.5 kg para comparar la curva de crecimiento de cada uno de estos géneros a lo largo del proceso, con mediciones en los días 0,1,2,3,4,7,14,28 y 56 de cerrado el silo. Se compararon las variaciones en el pH, la concentración de ácido acético, ácido láctico y azúcares reductores en los ensilajes de maíz y se evaluó la variación en la calidad nutric
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17

Journal, Baghdad Science. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer
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18

Al-Mathkhury, Harith J. F., and Alaa' Mohammed Hasan. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.2011.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer
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19

Pramanick, Rinku, Shraddha Parab, Niranjan Mayadeo, Himangi Warke, and Clara Aranha. "Cross sectional analysis of vaginal Lactobacillus in asymptomatic women of reproductive age in Mumbai, India." Journal of Infection in Developing Countries 12, no. 12 (2018): 1096–104. http://dx.doi.org/10.3855/jidc.10154.

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Introduction: Lactobacillus dominated vaginal microenvironment is associated with lower risk of genital infections. Numerous studies have reported geographic and ethnic variations in vaginal microbiome structure between healthy individuals from different race and ethnicity. India has a great diversity, so it is intriguing to find out if such divergences exist in vaginal lactobacilli. The present study aimed to investigate predominant Lactobacillus species in vaginas of healthy Indian women and screen isolates for lactic acid and H2O2 production.
 Methodology: 203 premenopausal women asymp
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20

Amirkhanova, Zhanerke, Saule Akhmetova, Samat Kozhakhmetov, et al. "Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan)." Open Access Macedonian Journal of Medical Sciences 9, A (2021): 827–32. http://dx.doi.org/10.3889/oamjms.2021.7053.

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BACKGROUND: It is a national priority to look for new probiotic bacteria with highly active biological properties to create a new generation of probiotics, ferments, therapeutic, and prophylactic fermented milk products, taking into account ethnocultural and regional characteristics. AIM: The aim of the study is to assess probiotic properties of strains of lactobacilli (antimicrobial and adhesive), which are isolated from national lactic acid products from different districts of the Karaganda region (Kazakhstan). MATERIALS AND METHODS: There were modern microbiological methods applied during t
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21

Mounia, Homrani, Dalache Fatiha, Bouzouina Mohammed, Nemmiche Said, and Homrani AbdelKader. "Antibacterial activity of Lactobacilli detected in Algerian raw honeys against gram-negative bacteria." South Asian Journal of Experimental Biology 8, no. 3 (2019): 83–90. http://dx.doi.org/10.38150/sajeb.8(3).p83-90.

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This study reports the isola on of Lactobacilli from Four (n=04) typical Apis Mellifeca raw honey samples collected from different regions in Algeria and the evalua on of their an bacterial ac vi es against Gram-negative bacteria. The isolation of Lactobacilli was performed using different media. Isolates were identified firrstly by catalase test, Gram staining and cells morphology, and confirmed by MALDI-TOF-MS. An bacterial activities of Bacterial cultures of selected Lactobacilli and their cell free supernatants were tested by agar spot assays and agar well diffusion assay. A total of eight
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22

St. Amant, Diane C., Iris E. Valentin-Bon, and Ann E. Jerse. "Inhibition of Neisseria gonorrhoeae by Lactobacillus Species That Are Commonly Isolated from the Female Genital Tract." Infection and Immunity 70, no. 12 (2002): 7169–71. http://dx.doi.org/10.1128/iai.70.12.7169-7171.2002.

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ABSTRACT Epidemiological studies suggest H2O2-producing lactobacilli protect women against gonorrhea. Here we demonstrate that Lactobacillus crispatus and Lactobacillus jensenii, the most common lactobacilli in the female genital tract, inhibit gonococci in both acidic and neutral pH conditions. Inhibition was neutralized by bovine catalase, suggesting that H2O2 is the primary mediator of inhibition.
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Guan, Le Luo, Karen E. Hagen, Gerald W. Tannock, Doug R. Korver, Gaylene M. Fasenko, and Gwen E. Allison. "Detection and Identification of Lactobacillus Species in Crops of Broilers of Different Ages by Using PCR-Denaturing Gradient Gel Electrophoresis and Amplified Ribosomal DNA Restriction Analysis." Applied and Environmental Microbiology 69, no. 11 (2003): 6750–57. http://dx.doi.org/10.1128/aem.69.11.6750-6757.2003.

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ABSTRACT The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production. Lactobacilli predominated and attained populations of 108 to 109 CFU per gram of crop contents. Many of the lactobacilli present in the crop (61.9% of isolates) belonged to species of the Lactobacillus
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24

Calonghi, N., C. Parolin, G. Sartor, et al. "Interaction of vaginal Lactobacillus strains with HeLa cells plasma membrane." Beneficial Microbes 8, no. 4 (2017): 625–33. http://dx.doi.org/10.3920/bm2016.0212.

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Vaginal lactobacilli offer protection against recurrent urinary and vaginal infections. The precise mechanisms underlying the interaction between lactobacilli and the host epithelium remain poorly understood at the molecular level. Deciphering such events can provide valuable information on the mode of action of commensal and probiotic bacteria in the vaginal environment. We investigated the effects exerted by five Lactobacillus strains of vaginal origin (Lactobacillus crispatus BC1 and BC2, Lactobacillus gasseri BC9 and BC11 and Lactobacillus vaginalis BC15) on the physical properties of the
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Lapirattanakul, Jinthana, Ryota Nomura, Rena Okawa, et al. "Oral Lactobacilli Related to Caries Status of Children with Primary Dentition." Caries Research 54, no. 2 (2020): 194–204. http://dx.doi.org/10.1159/000506468.

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Oral lactobacilli are members of a group of bacteria implicated in caries progression, although information regarding their transmission, colonization, and caries-associated species is not well established. This study isolated oral lactobacilli from a group of children with primary dentition for determination of Lactobacillus prevalence, detection of Streptococcus mutans, a major pathogen of caries initiation, and dental caries status of the children. Species of Lactobacillus isolates were determined from examination of 16S rDNA sequences. Subsequently, the most prevalent species was evaluated
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Radwan, R. Mohammed, R. Mohammed Maryam, and Y. Huseen Saja. "Antimicrobial Activity of Probiotic Lactobacilli against some Pathogenic Bacteria." Journal of Environmental and Occupational Health 12, no. 07 (2022): 237–41. https://doi.org/10.5281/zenodo.8146115.

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Probiotics are viable Lactic Acid Bacteria (LAB) that are believed to provide health benefits with administrated in appropriate quantities. Lactobacillus is one of the most genera of LAB that are known to produce substances including bacteriocins which can inhibit the growth of pathogenic bacteria. The current study determine the antibacterial activity probiotic lactobacilli again some clinical bacterial isolate. A commercially available probiotic lactobacilli product which contains (Lactobacillus acidophilus and Lactobacillus plantarum) strains were tested for their antibacterial activity aga
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27

Harina, E. I., U. A. Malakhova, O. S. Butrimova, and D. N. Chernov. "The comparative analysis of traditional and modernized nutrient media for the cultivation of lactobacilli." Sovremennaya nauka i innovatsii, no. 4 (44) (2023): 89–95. http://dx.doi.org/10.37493/2307-910x.2023.4.10.

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Culture media for lactobacilli cultivation are used for their growth and reproduction in laboratory conditions, as well as in the production of dairy products. The composition of the developed nutrient medium includes: skimmed milk, yeast extract, lactose, lactulose, agar-agar, distilled water. This complex of components provides lactobacilli with essential nutrients, including carbohydrates, proteins, vitamins and minerals. The constituent components of the nutrient medium are affordable and cheap to purchase. The work involved the cultivation of various types of lactobacilli. (Lactobacillus
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Settanni, Luca, Sara Valmorri, Douwe van Sinderen, Giovanna Suzzi, Antonello Paparella, and Aldo Corsetti. "Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species." Applied and Environmental Microbiology 72, no. 5 (2006): 3793–96. http://dx.doi.org/10.1128/aem.72.5.3793-3796.2006.

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ABSTRACT A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.
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Horáčková, Š., P. Sedláčková, M. Sluková, and M. Plocková. "The influence of whey, whey component and malt on the growth and acids production of lactobacilli in milk." Czech Journal of Food Sciences 32, No. 6 (2014): 526–31. http://dx.doi.org/10.17221/214/2014-cjfs.

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The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched
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30

Spurbeck, Rachel R., and Cindy Grove Arvidson. "Inhibition of Neisseria gonorrhoeae Epithelial Cell Interactions by Vaginal Lactobacillus Species." Infection and Immunity 76, no. 7 (2008): 3124–30. http://dx.doi.org/10.1128/iai.00101-08.

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ABSTRACT High levels of Lactobacillus, the dominant genus of the healthy human vaginal microbiota, have been epidemiologically linked to a reduced risk of infection following exposure to the sexually transmitted pathogen Neisseria gonorrhoeae. In this work, a cell culture model of gonococcal infection was adapted to examine the effects of lactobacilli on gonococcal interactions with endometrial epithelial cells in vitro. Precolonization of epithelial cells with Lactobacillus jensenii, Lactobacillus gasseri ATCC 33323, or L. gasseri ATCC 9857 reduced gonococcal adherence by nearly 50%. Lactobac
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Kabir, SM Lutful, SM Mushfiqur Rahman, Sucharit Basu Neogi, Mohummad Muklesur Rahman, and M. Shahidur Rahman Khan. "Isolation, identification, molecular characterization and screening of probiotic activities of Lactobacillus species from poultry sources at live bird markets in Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 54–65. http://dx.doi.org/10.3329/aajbb.v1i1.61531.

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A study was conducted to isolate and characterize Lactobacilli from the gastrointestinal (GI) tract of broiler chickens in different markets along with evaluation of probiotic ability and antibiotic sensitivity of Lactobacillus isolates during the period of July, 2014 to December, 2014. The caecum and cloacal contents of 100 broiler chickens from different markets were collected and cultured on Man Rogosa and Sharp (MRS) broth and agar. Lactobacilli were isolated and characterized by using phenotypic (cell morphology, Gram’s staining, physiological and biochemical tests which are specific for
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32

Fangous, M. S., Y. Alexandre, N. Hymery, et al. "Lactobacilli intra-tracheal administration protects from Pseudomonas aeruginosa pulmonary infection in mice – a proof of concept." Beneficial Microbes 10, no. 8 (2019): 893–900. http://dx.doi.org/10.3920/bm2019.0069.

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The spreading of antibiotic resistance is a major public health issue, which requires alternative treatments to antibiotics. Lactobacilli have shown abilities to prevent pneumonia in clinical studies when given by oral route, certainly through the gut-lung axis involvement. Rationally, respiratory administration of lactobacilli has been developed and studied in murine model, to prevent from respiratory pathogens. It allows a direct effect of probiotics into the respiratory system. To our knowledge, no study has ever focused on the effect of probiotic intra-respiratory administration to prevent
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Mokhtari, Sepideh, Saede Atarbashi-Moghadam, Elahe Motevaseli, Soudeh Ghafouri-Fard, and Ardeshir Hesampour. "Lactobacillus fermentum and Lactobacillus crispatus Do Not Have Cytotoxic Effects on HN5 Oral Squamous Cell Carcinoma Cell Line." International Journal of Dentistry 2021 (September 28, 2021): 1–6. http://dx.doi.org/10.1155/2021/3034068.

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Background. The oral environment has a very complex normal flora and a wide variety of bacteria including lactobacilli. Studies have shown oral microbial flora has important influence in the development of oral cancer. Squamous cell carcinomas account for more than 90% of cancers in oral cavity. Lactobacilli are known as one of the newest methods for the prevention and treatment of cancers. Previous studies on the effects of probiotics on oral cancer cells are very limited, and only two species of Lactobacillus which are not present in the normal oral microflora have been studied. Due to the u
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Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Analysis of Fecal Lactobacillus Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 72, no. 4 (2006): 2359–65. http://dx.doi.org/10.1128/aem.72.4.2359-2365.2006.

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ABSTRACT The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in great detail. For accurate enumeration of different lactobacilli, duplex 5′ nuclease assays, t
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Matsuguchi, Tetsuya, Akimitsu Takagi, Takeshi Matsuzaki, et al. "Lipoteichoic Acids from Lactobacillus Strains Elicit Strong Tumor Necrosis Factor Alpha-Inducing Activities in Macrophages through Toll-Like Receptor 2." Clinical Diagnostic Laboratory Immunology 10, no. 2 (2003): 259–66. http://dx.doi.org/10.1128/cdli.10.2.259-266.2003.

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ABSTRACT Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-α) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-κB activation and TNF-α production in RAW2
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Lysenko, Yuri, Albina Luneva, Margarita Yakovets, Evgeniy Marchenko, and Maria Lifentsova. "Study of morphological, biological and probiotic properties of autochthonous lactobacilli from the intestines of pheasants." BIO Web of Conferences 181 (2025): 01016. https://doi.org/10.1051/bioconf/202518101016.

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The work studied the morphological, biological and probiotic properties of representatives of the genus Lactobacillus (Loigolactobacillus coryniformis, Lactobacillus johnsonii, Lactobacillus reuteri), which were isolated from the contents of the caecum of domesticated pheasants using microbiological methods and identified to the species level using modern molecular genetic methods. It was found that when analyzing the morphological properties of the microorganisms, they had all the signs confirming their belonging to representatives of lactobacilli. It was revealed that Loigolactobacillus cory
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Borovkova, Ekaterina A., Elena V. Alieva, and Tatyana V. Frolova. "Biological Properties and Probiotic Potential of Intestinal Lactobacilli." Acta Biomedica Scientifica 4, no. 1 (2019): 124–32. http://dx.doi.org/10.29413/abs.2019-4.1.19.

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Data on inefficiency in some cases or temporary positive effect of commercial probiotics led to the development of the concept of autoprobiotic therapy. According to this, the correction of disturbed microbiocenoses is carried out using autostrains of indigenous normal flora (bifidobacteria, lactobacilli and enterococci). However, publications about effectiveness of autoprobiotic therapy are few and contradictory.The aim of the study was to investigate the biological properties and probiotic potential of intestinal lactobacilli, as well as the effectiveness of autoprobiotic therapy, based on L
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Bhattacharyya, Barun K., S. Chowdhury, S. Das, et al. "Selective Enumeration of Lactobacillus acidophilus for Probiotic Formulations." International Journal of Pharmaceutical Sciences and Nanotechnology 7, no. 4 (2014): 2646–50. http://dx.doi.org/10.37285/ijpsn.2014.7.4.6.

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Probiotic microorganisms have been utilized for many years in food processing, preservations and for nutraceutical health benefits. There are a large number of pharmaceutical formulations containing probiotic microorganisms available in the market worldwide. Majority of these organisms are the members specifically from genera Lactobacillus, Lactococcus and Bifidobacterium. A number of media have been proposed for selective or differential enumeration of lactobacilli and bifidobacteria in mixed populations. The development of selective medium using different types of ingredients and antibiotic
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Strashnova, I. V., G. V. Yamborko, and N. Yu Vasylieva. "ANTAGONISTIC ACTIVITY OF LACTOBACILLI PROBIOTIC STRAINS IN CO-CULTIVATION." Microbiology&Biotechnology, no. 1(54) (April 18, 2022): 45–57. http://dx.doi.org/10.18524/2307-4663.2022.1(54).254024.

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SummaryAim. To investigate the antagonistic properties of multistrain compositions and consortia created on the basis of probiotic strains of bacteria of the genus Lactobacillus. Methods. A biocompatibility was investigated by co-cultivation of lactobacilli strains on MRS-agar medium. To create the compositions used separately grown daily broth cultures in certain proportions, to create consortia - strains of lactobacilli in the appropriate proportions were cultivated together in MRS broth during 24 h. The antagonistic activity of the created combinations against 10 test cultures was determine
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Stojanov, Spase, Julijana Kristl, Špela Zupančič, and Aleš Berlec. "Influence of Excipient Composition on Survival of Vaginal Lactobacilli in Electrospun Nanofibers." Pharmaceutics 14, no. 6 (2022): 1155. http://dx.doi.org/10.3390/pharmaceutics14061155.

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The lack of appropriate delivery systems hinders the use of probiotics in the treatment of vaginal infections. Therefore, the development of a new delivery system for the local administration of vaginal probiotics is necessary. In this study, we selected three vaginal lactobacilli, i.e., Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus jensenii, and incorporated them into nanofibers using electrospinning. Polyethylene oxide (PEO) was used as a carrier polymer to produce nanofibers. It was supplemented with alginate and sucrose selected from a group of carbohydrates for their g
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Henri-Dubernet, Ségolène, Nathalie Desmasures, and Micheline Guéguen. "Diversity and dynamics of lactobacilli populations during ripening of RDO Camembert cheese." Canadian Journal of Microbiology 54, no. 3 (2008): 218–28. http://dx.doi.org/10.1139/w07-137.

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The diversity and dynamics of Lactobacillus populations in traditional raw milk Camembert cheese were monitored throughout the manufacturing process in 3 dairies. Culture-dependent analysis was carried out on isolates grown on acidified de Man – Rogosa – Sharpe agar and Lactobacillus anaerobic de Man Rogosa Sharpe agar supplemented with vancomycin and bromocresol green media. The isolates were identified by polymerase chain reaction – temperature gradient gel electrophoresis (PCR–TGGE) and (or) species-specific PCR and (or) sequencing, and Lactobacillus paracasei and Lactobacillus plantarum is
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Azeem, Nimra, Muhammad Nawaz, Aftab Ahmad Anjum, et al. "Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant." Animals 9, no. 4 (2019): 166. http://dx.doi.org/10.3390/ani9040166.

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Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 pr
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Smidt, I., R. Kiiker, H. Oopkaup, et al. "Comparison of detection methods for vaginal lactobacilli." Beneficial Microbes 6, no. 5 (2015): 747–51. http://dx.doi.org/10.3920/bm2014.0154.

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Vaginal lactobacilli offer protection against microbiota imbalance and genitourinary tract infections. We compared vaginal lactobacilli in 50 Estonian women of child-bearing age applying culture-based methods, quantitative PCR and next-generation sequencing (NGS). The culture-based methods found three different lactobacilli: Lactobacillus crispatus, Lactobacillus jensenii and Lactobacillus gasseri. Using NGS revealed the presence of L. crispatus in 76%, Lactobacillus iners in 52%, L. jensenii in 47% and L. gasseri in 33% of the samples. According to qPCR, L. iners was present in 67% and L. cri
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Robledo-Cardona, Simón, Sabina Ramírez-Hincapié, and Javier Correa-Álvarez. "Implementation of a Non-Invasive Bioprospecting Protocol for Isolation of Lactobacillus from Feces of Hens Under Foraging Conditions." Ingeniería y Ciencia 14, no. 28 (2018): 93–111. http://dx.doi.org/10.17230/ingciencia.14.28.4.

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In animal production, probiotics seek to replace the use of antibiotics, while diminishing mortality and morbidity rates to raise productivity. Probiotics constitute a natural alternative that, in contrast with antibiotics, neither produces pathogen resistance, nor leaves chemical residues in the final product. Several bacteria, including some belonging to the genus Lactobacillus have been described as probiotics with high potential. A non-invasive bioprospecting protocol aimed for the isolation and characterization of lactobacilli from chicken feces was established. Fecal samples were collect
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Trivedi, Disha, Prasant Kumar Jena, and Sriram Seshadri. "Colicin E2 Expression in Lactobacillus brevis DT24, A Vaginal Probiotic Isolate, against Uropathogenic Escherichia coli." ISRN Urology 2014 (February 4, 2014): 1–7. http://dx.doi.org/10.1155/2014/869610.

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Novel therapeutic approaches are needed to combat the urinary tract infection in women. During menstruation elevated protein concentration and increase in oxygen and carbon dioxide concentrations with decrease in vaginal Lactobacilli all together contribute to urinary tract infections. Lactobacillus species are a predominant member of the vaginal microflora and are critical in the prevention of a number of urogenital diseases. In order to increase antimicrobial potential of vaginal Lactobacilli, bacteriocin colicin E2 which has specific activity against uropathogenic Escherichia coli has been
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Vitali, Beatrice, Ciro Pugliese, Elena Biagi, et al. "Dynamics of Vaginal Bacterial Communities in Women Developing Bacterial Vaginosis, Candidiasis, or No Infection, Analyzed by PCR-Denaturing Gradient Gel Electrophoresis and Real-Time PCR." Applied and Environmental Microbiology 73, no. 18 (2007): 5731–41. http://dx.doi.org/10.1128/aem.01251-07.

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ABSTRACT The microbial flora of the vagina plays a major role in preventing genital infections, including bacterial vaginosis (BV) and candidiasis (CA). An integrated approach based on PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR was used to study the structure and dynamics of bacterial communities in vaginal fluids of healthy women and patients developing BV and CA. Universal eubacterial primers and Lactobacillus genus-specific primers, both targeted at 16S rRNA genes, were used in DGGE and real-time PCR analysis, respectively. The DGGE profiles revealed that the v
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Lidberg, Kenny, Sarah Pilheden, Mikel Relloso Ortiz de Uriarte, and Ann-Beth Jonsson. "Internalization of Lactobacillus crispatus Through Caveolin-1-Mediated Endocytosis Boosts Cellular Uptake but Blocks the Transcellular Passage of Neisseria meningitidis." Microorganisms 13, no. 3 (2025): 479. https://doi.org/10.3390/microorganisms13030479.

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Neisseria meningitidis is a human-specific pathogen that colonizes the nasopharyngeal epithelium, which is populated by a dynamic microbiota that includes Lactobacillus species. Currently, little is known about the interaction between commensal lactobacilli and pathogenic Neisseria, emphasizing a need for deeper studies into the molecular interactions between the two bacteria species. This, in turn, could add clinical and therapeutic value to existing treatments against an N. meningitidis infection. In this work, we explored how lactobacilli affect the interplay between N. meningitidis and hos
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Zheng, Jinshui, Stijn Wittouck, Elisa Salvetti, et al. "A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae." International Journal of Systematic and Evolutionary Microbiology 70, no. 4 (2020): 2782–858. http://dx.doi.org/10.1099/ijsem.0.004107.

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The genus Lactobacillus comprises 261 species (at March 2020) that are extremely diverse at phenotypic, ecological and genotypic levels. This study evaluated the taxonomy of Lactobacillaceae and Leuconostocaceae on the basis of whole genome sequences. Parameters that were evaluated included core genome phylogeny, (conserved) pairwise average amino acid identity, clade-specific signature genes, physiological criteria and the ecology of the organisms. Based on this polyphasic approach, we propose reclassification of the genus Lactobacillus into 25 genera including the emended genus Lactobacillus
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Takahashi, Hidenori, Yasuhiro Nakano, Takashi Matsuoka, Nobue Kumaki, Yukio Asami, and Yasuhiro Koga. "Role of Indigenous Lactobacilli in Gastrin-Mediated Acid Production in the Mouse Stomach." Applied and Environmental Microbiology 77, no. 19 (2011): 6964–71. http://dx.doi.org/10.1128/aem.05230-11.

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ABSTRACTIt is known that the stomach is colonized by indigenous lactobacilli in mice. The aim of this study was to examine the role of such lactobacilli in the development of the stomach. For a DNA microarray analysis, germ-free BALB/c mice were orally inoculated with 109CFU lactobacilli, and their stomachs were excised after 10 days to extract RNA. As a result, lactobacillus-associated gnotobiotic mice showed dramatically decreased expression of the gastrin gene in comparison to germ-free mice. The mean of the log2fold change in the gastrin gene was −4.3. Immunohistochemistry also demonstrate
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das Neves Selis, Nathan, Hellen Braga Martins de Oliveira, Yan Bento dos Anjos, et al. "Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit." BioMed Research International 2021 (April 29, 2021): 1–15. http://dx.doi.org/10.1155/2021/6626249.

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In recent years, certain Lactobacillus sp. have emerged in health care as an alternative therapy for various diseases. Based on this, this study is aimed at evaluating in vitro the potential probiotics of five lactobacilli strains isolated from pulp of cupuaçu fruit fermentation against Gardnerella vaginalis and Neisseria gonorrhoeae. Our lactobacilli strains were classified as safe for use in humans, and they were tolerant to heat and pH. Our strains were biofilm producers, while hydrophobicity and autoaggregation varied from 13% to 86% and 13% to 25%, respectively. The coaggregation of lacto
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