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Journal articles on the topic 'Lactobacillus'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Chen, Chunfei, Leilei Yu, Fengwei Tian, Jianxin Zhao, and Qixiao Zhai. "Identification of Novel Bile Salt-Tolerant Genes in Lactobacillus Using Comparative Genomics and Its Application in the Rapid Screening of Tolerant Strains." Microorganisms 10, no. 12 (2022): 2371. http://dx.doi.org/10.3390/microorganisms10122371.

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Under bile salt treatment, strains display significant differences in their tolerance ability, suggesting the existence of diverse resistance mechanisms in Lactobacillus; however, the genes involved in this protective process are not fully understood. In this study, novel target genes associated with bile salt tolerance in Lactobacillus were identified using comparative genomics for PCR detection and the rapid screening of tolerant strains. The bile salt tolerance of 107 lactobacilli isolated from different origins was assessed, and 26 strains with comparatively large differences were selected

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2

Výrostková, Jana, Ivana Regecová, Mariana Kováčová, Slavomír Marcinčák, Eva Dudriková, and Jana Maľová. "Antimicrobial Resistance of Lactobacillus johnsonii and Lactobacillus zeae in Raw Milk." Processes 8, no. 12 (2020): 1627. http://dx.doi.org/10.3390/pr8121627.

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Lactobacillus johnsonii and Lactobacillus zeae are among the lactobacilli with probiotic properties, which occur in sour milk products, cheeses, and to a lesser extent in raw milk. Recently, resistant strains have been detected in various species of lactobacilli. The aim of the study was to determine the incidence of resistant Lactobacillus johnsonii and Lactobacillus zeae strains in various types of raw milk. A total of 245 isolates were identified by matrix-assisted laser desorption/ionization mass spectrometry and polymerase chain reaction methods as Lactobacillus sp., of which 23 isolates

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3

Bilkova, A., H. Kinova Sepova, M. Bukovsky, and L. Bezakova. "Antibacterial potential of lactobacilli isolated from a lamb." Veterinární Medicína 56, No. 7 (2011): 319–24. http://dx.doi.org/10.17221/1583-vetmed.

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The antimicrobial properties of three potential probiotic strains of lactobacilli isolated from a lamb (Lactobacillus murinus C, Lactobacillus mucosae D and Lactobacillus reuteri E) were studied using the streak line method and the agar well diffusion assay. The probiotic lactobacilli strains Lactobacillus rhamnosus ATCC 53103, Lactobacillus reuteri ATCC 55730, Lactobacillus reuteri ATCC 55845 and Lactobacillus plantarum DSM 9843 were used for comparison. Using the streak line method the inhibitory activity of lactobacilli products towards ten Gram-positive and Gram-negative potential pathogen

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4

Bobcek, B., Ľ. Gajdosová, M. Kacániová, J. Lejková, L. Hleba, and V. Kmeť. "Antibacterial susceptibility of lactobacilli isolated from pork meat of conventional breeding." Biotehnologija u stocarstvu 27, no. 3 (2011): 799–807. http://dx.doi.org/10.2298/bah1103799b.

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The aim of this study was monitoring of antibiotic resistance of Enterobacteriaceae genera isolated from musculus longissimus at thoracis and musculus semimembranosus of pork. The pigs was feeding in two groups (control and experimental wit selenium application). For the antibiotic susceptibility testing disk diffusion method was used. Lactobacillus species were tested against two antibiotics: ampiciline, tetracycline. For the detection and identification of lactobacilli we used MRS and Rogose agar. For identification of lactobacilli MALDI-TOF-MS were used. The higher resistance of isolated ba

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Kabir, SM Lutful, SM Mushfiqur Rahman, Sucharit Basu Neogi, Mohummad Muklesur Rahman, and M. Shahidur Rahman Khan. "Isolation, identification, molecular characterization and screening of probiotic activities of Lactobacillus species from poultry sources at live bird markets in Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 54–65. http://dx.doi.org/10.3329/aajbb.v1i1.61531.

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A study was conducted to isolate and characterize Lactobacilli from the gastrointestinal (GI) tract of broiler chickens in different markets along with evaluation of probiotic ability and antibiotic sensitivity of Lactobacillus isolates during the period of July, 2014 to December, 2014. The caecum and cloacal contents of 100 broiler chickens from different markets were collected and cultured on Man Rogosa and Sharp (MRS) broth and agar. Lactobacilli were isolated and characterized by using phenotypic (cell morphology, Gram’s staining, physiological and biochemical tests which are specific for

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6

Henri-Dubernet, Ségolène, Nathalie Desmasures, and Micheline Guéguen. "Diversity and dynamics of lactobacilli populations during ripening of RDO Camembert cheese." Canadian Journal of Microbiology 54, no. 3 (2008): 218–28. http://dx.doi.org/10.1139/w07-137.

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The diversity and dynamics of Lactobacillus populations in traditional raw milk Camembert cheese were monitored throughout the manufacturing process in 3 dairies. Culture-dependent analysis was carried out on isolates grown on acidified de Man – Rogosa – Sharpe agar and Lactobacillus anaerobic de Man Rogosa Sharpe agar supplemented with vancomycin and bromocresol green media. The isolates were identified by polymerase chain reaction – temperature gradient gel electrophoresis (PCR–TGGE) and (or) species-specific PCR and (or) sequencing, and Lactobacillus paracasei and Lactobacillus plantarum is

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7

Dong, Jie, Yun Sheng Jiang, Hai Yu, Wen Juan Zhang, and Yu Feng Liu. "Study on Lactobacillus Isolation from Rabbit Meat Sausage and its Fermentation Property." Applied Mechanics and Materials 140 (November 2011): 431–40. http://dx.doi.org/10.4028/www.scientific.net/amm.140.431.

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Three lactobacilli (L26, L34 , L106) isolated from naturally fermented rabbit sausage in a traditional way are identified as lactobacillus plantarum, lactobacillus sake and lactobacillus fructosus. Further study of its fermentation property shows that three lactobacilli are effective in acid production, alkali resistant, nitrite resistant and bacteriostatic, and are antagonisticaction free, which can be used as combined rabbit meat starter.

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8

Stojanov, Spase, Tina Vida Plavec, Julijana Kristl, Špela Zupančič, and Aleš Berlec. "Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers." International Journal of Molecular Sciences 22, no. 24 (2021): 13631. http://dx.doi.org/10.3390/ijms222413631.

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Lactobacilli are a promising natural tool against vaginal dysbiosis and infections. However, new local delivery systems and additional knowledge about their distribution and mechanism of action would contribute to the development of effective medicine. This will be facilitated by the introduction of the techniques for effective, inexpensive, and real-time tracking of these probiotics following their release. Here, we engineered three model vaginal lactobacilli (Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacill

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9

Liu, Fuyi, Jingbo Chang, Jingqi Huang, et al. "Surface Display of Avian H5 and H9 Hemagglutinin Antigens on Non-Genetically Modified Lactobacillus Cells for Bivalent Oral AIV Vaccine Development." Microorganisms 13, no. 7 (2025): 1649. https://doi.org/10.3390/microorganisms13071649.

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A novel bivalent oral vaccine candidate against H5N1 and H9N2 avian influenza virus (AIV) was developed using Lactobacillus surface display technology without genetic modification. The hemagglutinin subunit 1 (HA1) antigens from both subtypes were fused to the surface layer-binding domain of Lactobacillus crispatus K313, expressed in Escherichia coli, and purified. Wild-type Lactobacillus johnsonii H31, isolated from chicken intestine, served as a delivery vehicle by adsorbing and stably displaying the HA1 proteins on its surface. This approach eliminates the need for bacterial engineering whi

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Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Analysis of Fecal Lactobacillus Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 72, no. 4 (2006): 2359–65. http://dx.doi.org/10.1128/aem.72.4.2359-2365.2006.

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ABSTRACT The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in great detail. For accurate enumeration of different lactobacilli, duplex 5′ nuclease assays, t

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Zheng, Jinshui, Stijn Wittouck, Elisa Salvetti, et al. "A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae." International Journal of Systematic and Evolutionary Microbiology 70, no. 4 (2020): 2782–858. http://dx.doi.org/10.1099/ijsem.0.004107.

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The genus Lactobacillus comprises 261 species (at March 2020) that are extremely diverse at phenotypic, ecological and genotypic levels. This study evaluated the taxonomy of Lactobacillaceae and Leuconostocaceae on the basis of whole genome sequences. Parameters that were evaluated included core genome phylogeny, (conserved) pairwise average amino acid identity, clade-specific signature genes, physiological criteria and the ecology of the organisms. Based on this polyphasic approach, we propose reclassification of the genus Lactobacillus into 25 genera including the emended genus Lactobacillus

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12

Ghazaei, Ciamak. "Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria." Research in Molecular Medicine 8, no. 4 (2020): 189–200. http://dx.doi.org/10.32598/rmm.8.4.4.

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Background: Probiotics are “live microbial cells” that are beneficial for human and animal health. Lactobacilli are such a diverse group of bacteria with similar metabolic and physiological characteristics, and constitute important and beneficial gut microflora. During carbohydrate fermentation, lactobacilli produce lactic acid as an end product in metabolism. Hence, lactobacilli have high significance to be used as probiotics in the food industry, because of their acidifying properties. Also, lactobacilli are considered “safe”, owing to their ubiquitous presence in the food. Many researchers

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13

Guan, Le Luo, Karen E. Hagen, Gerald W. Tannock, Doug R. Korver, Gaylene M. Fasenko, and Gwen E. Allison. "Detection and Identification of Lactobacillus Species in Crops of Broilers of Different Ages by Using PCR-Denaturing Gradient Gel Electrophoresis and Amplified Ribosomal DNA Restriction Analysis." Applied and Environmental Microbiology 69, no. 11 (2003): 6750–57. http://dx.doi.org/10.1128/aem.69.11.6750-6757.2003.

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ABSTRACT The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production. Lactobacilli predominated and attained populations of 108 to 109 CFU per gram of crop contents. Many of the lactobacilli present in the crop (61.9% of isolates) belonged to species of the Lactobacillus

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Horáčková, Š., P. Sedláčková, M. Sluková, and M. Plocková. "The influence of whey, whey component and malt on the growth and acids production of lactobacilli in milk." Czech Journal of Food Sciences 32, No. 6 (2014): 526–31. http://dx.doi.org/10.17221/214/2014-cjfs.

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The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched

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15

MURAD, H. A., R. I. REFAEA та E. M. ALY. "Utilization of UF-Permeate for Production of β-galactosidase by Lactic Acid Bacteria". Polish Journal of Microbiology 60, № 2 (2011): 139–44. http://dx.doi.org/10.33073/pjm-2011-019.

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Four lactobacilli strains (Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacilus casei and Lactobacillus reuteri) were grown in MRS broth and three lactococci strains (Streptococcus thermophilus, Lactococcus lactis subsp. Lactis and Lactococcus lactis subsp. lactis biovar. diacetilactis) were grown in M17 broth. L. reuteri and S. thermophilus were chosen on the basis of the best mean beta-galactosidase activity of 10.44 and 10.01 U/ml respectively, for further studies on permeate-based medium. The maximum production of beta-galactosidase by L. reuteri was achieved at lactose concen

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Balogun, S. A., Ogunbanwo, S.T., and Oni, F. F. "Screening for Biosurfactant Production from Lactic Acid Bacteria isolated from African fermented milk ‘Wara’." UMYU Journal of Microbiology Research (UJMR) 2, no. 1 (2017): 36–41. http://dx.doi.org/10.47430/ujmr.1721.006.

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Lactic Acid Bacteria (LAB) from ‘wara’ an African fermented milk (cheese)was screened for the production of surface-active agent (biosurfactant) from five species of Lactobacillus. Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus casei, and Lactococcus lactis using red blood cell haemolysis test, emulsification index and oil spreading assay. Lactobacillus fermentum and Lactobacilli brevis showed the largest zone of heamolysis when screened on blood agar. The highest emulsification index of 20% was by Lactobacillus plantarum while the least emulsification in

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Amirkhanova, Zhanerke, Saule Akhmetova, Samat Kozhakhmetov, et al. "Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan)." Open Access Macedonian Journal of Medical Sciences 9, A (2021): 827–32. http://dx.doi.org/10.3889/oamjms.2021.7053.

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BACKGROUND: It is a national priority to look for new probiotic bacteria with highly active biological properties to create a new generation of probiotics, ferments, therapeutic, and prophylactic fermented milk products, taking into account ethnocultural and regional characteristics. AIM: The aim of the study is to assess probiotic properties of strains of lactobacilli (antimicrobial and adhesive), which are isolated from national lactic acid products from different districts of the Karaganda region (Kazakhstan). MATERIALS AND METHODS: There were modern microbiological methods applied during t

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Strashnova, I. V., G. V. Yamborko, and N. Yu Vasylieva. "ANTAGONISTIC ACTIVITY OF LACTOBACILLI PROBIOTIC STRAINS IN CO-CULTIVATION." Microbiology&Biotechnology, no. 1(54) (April 18, 2022): 45–57. http://dx.doi.org/10.18524/2307-4663.2022.1(54).254024.

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SummaryAim. To investigate the antagonistic properties of multistrain compositions and consortia created on the basis of probiotic strains of bacteria of the genus Lactobacillus. Methods. A biocompatibility was investigated by co-cultivation of lactobacilli strains on MRS-agar medium. To create the compositions used separately grown daily broth cultures in certain proportions, to create consortia - strains of lactobacilli in the appropriate proportions were cultivated together in MRS broth during 24 h. The antagonistic activity of the created combinations against 10 test cultures was determine

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DRAKE, MARYANNE, CHRISTOPHER L. SMALL, KEMET D. SPENCE, and BARRY G. SWANSON. "Rapid Detection and Identification of Lactobacillus spp. in Dairy Products by Using the Polymerase Chain Reaction." Journal of Food Protection 59, no. 10 (1996): 1031–36. http://dx.doi.org/10.4315/0362-028x-59.10.1031.

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Species-specific primers for use in the polymerase chain reaction (PCR) were designed to differentially amplify DNA from the common dairy lactobacillus species Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus helveticus, and Lactobacillus acidophilus. A method for rapid extraction of bacterial DNA from dairy products was developed. The sensitivity of bacterial DNA extraction from food and subsequent amplification by PCR was 100 cells total. Lactobacillus DNA was extracted and identified from commercial yoghurts, acidophilus milk, and cheeses. The methodology allows the presumptive

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Calonghi, N., C. Parolin, G. Sartor, et al. "Interaction of vaginal Lactobacillus strains with HeLa cells plasma membrane." Beneficial Microbes 8, no. 4 (2017): 625–33. http://dx.doi.org/10.3920/bm2016.0212.

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Vaginal lactobacilli offer protection against recurrent urinary and vaginal infections. The precise mechanisms underlying the interaction between lactobacilli and the host epithelium remain poorly understood at the molecular level. Deciphering such events can provide valuable information on the mode of action of commensal and probiotic bacteria in the vaginal environment. We investigated the effects exerted by five Lactobacillus strains of vaginal origin (Lactobacillus crispatus BC1 and BC2, Lactobacillus gasseri BC9 and BC11 and Lactobacillus vaginalis BC15) on the physical properties of the

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Lapirattanakul, Jinthana, Ryota Nomura, Rena Okawa, et al. "Oral Lactobacilli Related to Caries Status of Children with Primary Dentition." Caries Research 54, no. 2 (2020): 194–204. http://dx.doi.org/10.1159/000506468.

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Oral lactobacilli are members of a group of bacteria implicated in caries progression, although information regarding their transmission, colonization, and caries-associated species is not well established. This study isolated oral lactobacilli from a group of children with primary dentition for determination of Lactobacillus prevalence, detection of Streptococcus mutans, a major pathogen of caries initiation, and dental caries status of the children. Species of Lactobacillus isolates were determined from examination of 16S rDNA sequences. Subsequently, the most prevalent species was evaluated

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Matsuguchi, Tetsuya, Akimitsu Takagi, Takeshi Matsuzaki, et al. "Lipoteichoic Acids from Lactobacillus Strains Elicit Strong Tumor Necrosis Factor Alpha-Inducing Activities in Macrophages through Toll-Like Receptor 2." Clinical Diagnostic Laboratory Immunology 10, no. 2 (2003): 259–66. http://dx.doi.org/10.1128/cdli.10.2.259-266.2003.

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ABSTRACT Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-α) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-κB activation and TNF-α production in RAW2

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Lysenko, Yuri, Albina Luneva, Margarita Yakovets, Evgeniy Marchenko, and Maria Lifentsova. "Study of morphological, biological and probiotic properties of autochthonous lactobacilli from the intestines of pheasants." BIO Web of Conferences 181 (2025): 01016. https://doi.org/10.1051/bioconf/202518101016.

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The work studied the morphological, biological and probiotic properties of representatives of the genus Lactobacillus (Loigolactobacillus coryniformis, Lactobacillus johnsonii, Lactobacillus reuteri), which were isolated from the contents of the caecum of domesticated pheasants using microbiological methods and identified to the species level using modern molecular genetic methods. It was found that when analyzing the morphological properties of the microorganisms, they had all the signs confirming their belonging to representatives of lactobacilli. It was revealed that Loigolactobacillus cory

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Karlsson, M., and J. Jass. "Lactobacilli differently regulate expression and secretion of CXCL8 in urothelial cells." Beneficial Microbes 3, no. 3 (2012): 195–203. http://dx.doi.org/10.3920/bm2012.0011.

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Modulation of the immune response is an established feature of certain lactobacilli. CXCL8 is an inflammatory chemokine released by the urinary tract mucosa after contact with uropathogenic Escherichia coli during urinary tract infection and is crucial for proper infiltration of immune cells. Nevertheless, persistently high levels of CXCL8 are associated with pathogenicity and malignancy. In this study, we tested twelve Lactobacillus strains for their ability to influence CXCL8 release from urothelial cells. We evaluated how strains from different Lactobacillus species could regulate CXCL8 in

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Farahmand, Nasim, Labia I. I. Ouoba, Shahram Naghizadeh Raeisi, Jane Sutherland, and Hamid B. Ghoddusi. "Probiotic Lactobacilli in Fermented Dairy Products: Selective Detection, Enumeration and Identification Scheme." Microorganisms 9, no. 8 (2021): 1600. http://dx.doi.org/10.3390/microorganisms9081600.

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A selection of 36 commercial probiotic fermented dairy products from UK and Europe markets were evaluated for the numbers, types, and viability of Lactobacillus strains against the stated information on their packages. A comparative study was carried out on selectivity of MRS-Clindamycin, MRS-Sorbitol, and MRS-IM Maltose, to select the right medium for enumeration of probiotic Lactobacillus. Based on selectivity of medium for recovery of the targeted lactobacilli, and also simplicity of preparation, MRS-Clindamycin was chosen as the best medium for enumeration of probiotic Lactobacillus in fer

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Azeem, Nimra, Muhammad Nawaz, Aftab Ahmad Anjum, et al. "Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant." Animals 9, no. 4 (2019): 166. http://dx.doi.org/10.3390/ani9040166.

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Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 pr

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CHANG, M. H., and T. C. CHEN. "Reduction of Campylobacter jejuni in a Simulated Chicken Digestive Tract by Lactobacilli Cultures." Journal of Food Protection 63, no. 11 (2000): 1594–97. http://dx.doi.org/10.4315/0362-028x-63.11.1594.

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Studies were conducted to investigate the impact of a selected lactobacilli mixed culture on Campylobacter jejuni in simulated chicken digestive tract models. Veronal buffer solutions corresponding to the pH of successive segments of the chicken digestive tract were prepared. The lactobacilli mixtures were prepared by mixing four fresh lactobacilli cultures, including Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus crispatus, and Lactobacillus brevis. The C. jejuni and lactobacilli mixture were mixed with sterile poultry feed, and the previously prepared veronal buffer soluti

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St. Amant, Diane C., Iris E. Valentin-Bon, and Ann E. Jerse. "Inhibition of Neisseria gonorrhoeae by Lactobacillus Species That Are Commonly Isolated from the Female Genital Tract." Infection and Immunity 70, no. 12 (2002): 7169–71. http://dx.doi.org/10.1128/iai.70.12.7169-7171.2002.

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ABSTRACT Epidemiological studies suggest H2O2-producing lactobacilli protect women against gonorrhea. Here we demonstrate that Lactobacillus crispatus and Lactobacillus jensenii, the most common lactobacilli in the female genital tract, inhibit gonococci in both acidic and neutral pH conditions. Inhibition was neutralized by bovine catalase, suggesting that H2O2 is the primary mediator of inhibition.

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Radwan, R. Mohammed, R. Mohammed Maryam, and Y. Huseen Saja. "Antimicrobial Activity of Probiotic Lactobacilli against some Pathogenic Bacteria." Journal of Environmental and Occupational Health 12, no. 07 (2022): 237–41. https://doi.org/10.5281/zenodo.8146115.

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Probiotics are viable Lactic Acid Bacteria (LAB) that are believed to provide health benefits with administrated in appropriate quantities. Lactobacillus is one of the most genera of LAB that are known to produce substances including bacteriocins which can inhibit the growth of pathogenic bacteria. The current study determine the antibacterial activity probiotic lactobacilli again some clinical bacterial isolate. A commercially available probiotic lactobacilli product which contains (Lactobacillus acidophilus and Lactobacillus plantarum) strains were tested for their antibacterial activity aga

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Harina, E. I., U. A. Malakhova, O. S. Butrimova, and D. N. Chernov. "The comparative analysis of traditional and modernized nutrient media for the cultivation of lactobacilli." Sovremennaya nauka i innovatsii, no. 4 (44) (2023): 89–95. http://dx.doi.org/10.37493/2307-910x.2023.4.10.

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Culture media for lactobacilli cultivation are used for their growth and reproduction in laboratory conditions, as well as in the production of dairy products. The composition of the developed nutrient medium includes: skimmed milk, yeast extract, lactose, lactulose, agar-agar, distilled water. This complex of components provides lactobacilli with essential nutrients, including carbohydrates, proteins, vitamins and minerals. The constituent components of the nutrient medium are affordable and cheap to purchase. The work involved the cultivation of various types of lactobacilli. (Lactobacillus

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Pramanick, Rinku, Shraddha Parab, Niranjan Mayadeo, Himangi Warke, and Clara Aranha. "Cross sectional analysis of vaginal Lactobacillus in asymptomatic women of reproductive age in Mumbai, India." Journal of Infection in Developing Countries 12, no. 12 (2018): 1096–104. http://dx.doi.org/10.3855/jidc.10154.

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Introduction: Lactobacillus dominated vaginal microenvironment is associated with lower risk of genital infections. Numerous studies have reported geographic and ethnic variations in vaginal microbiome structure between healthy individuals from different race and ethnicity. India has a great diversity, so it is intriguing to find out if such divergences exist in vaginal lactobacilli. The present study aimed to investigate predominant Lactobacillus species in vaginas of healthy Indian women and screen isolates for lactic acid and H2O2 production. Methodology: 203 premenopausal women asymp

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Trivedi, Disha, Prasant Kumar Jena, and Sriram Seshadri. "Colicin E2 Expression in Lactobacillus brevis DT24, A Vaginal Probiotic Isolate, against Uropathogenic Escherichia coli." ISRN Urology 2014 (February 4, 2014): 1–7. http://dx.doi.org/10.1155/2014/869610.

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Novel therapeutic approaches are needed to combat the urinary tract infection in women. During menstruation elevated protein concentration and increase in oxygen and carbon dioxide concentrations with decrease in vaginal Lactobacilli all together contribute to urinary tract infections. Lactobacillus species are a predominant member of the vaginal microflora and are critical in the prevention of a number of urogenital diseases. In order to increase antimicrobial potential of vaginal Lactobacilli, bacteriocin colicin E2 which has specific activity against uropathogenic Escherichia coli has been

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Szydłowska, Aleksandra, and Dorota Zielińska. "Wpływ dodatku przyprawy z suszonej kory cynamonowca na przeżywalność potencjalnie probiotycznych szczepów bakterii w musach dyniowo-jabłkowych i ich jakość sensoryczną." Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality 113, no. 4 (2017): 48–58. http://dx.doi.org/10.15193/zntj/2017/113/210.

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Celem pracy było określenie wpływu dodatku cynamonu na przeżywalność dwóch potencjalnie probiotycznych szczepów bakterii: Lactobacillis rhamnosus K3, Lactobacillus brevis O24 oraz na jakość sensoryczną probiotycznych musów dyniowo-jabłkowych, przechowywanych w temp. 5 ºC przez 21 dni. Zakres pracy obejmował produkcję probiotycznych musów dyniowo-jabłkowych w warunkach laboratoryjnych, oznaczenie liczby bakterii dwóch wymienionych szczepów, pomiar wartości pH oraz ocenę jakości sensorycznej produktów. Na podstawie przeprowadzonych badań stwierdzono istotny wpływ czasu przechowywania na liczbę b

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Settanni, Luca, Sara Valmorri, Douwe van Sinderen, Giovanna Suzzi, Antonello Paparella, and Aldo Corsetti. "Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species." Applied and Environmental Microbiology 72, no. 5 (2006): 3793–96. http://dx.doi.org/10.1128/aem.72.5.3793-3796.2006.

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ABSTRACT A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

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Mounia, Homrani, Dalache Fatiha, Bouzouina Mohammed, Nemmiche Said, and Homrani AbdelKader. "Antibacterial activity of Lactobacilli detected in Algerian raw honeys against gram-negative bacteria." South Asian Journal of Experimental Biology 8, no. 3 (2019): 83–90. http://dx.doi.org/10.38150/sajeb.8(3).p83-90.

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This study reports the isola on of Lactobacilli from Four (n=04) typical Apis Mellifeca raw honey samples collected from different regions in Algeria and the evalua on of their an bacterial ac vi es against Gram-negative bacteria. The isolation of Lactobacilli was performed using different media. Isolates were identified firrstly by catalase test, Gram staining and cells morphology, and confirmed by MALDI-TOF-MS. An bacterial activities of Bacterial cultures of selected Lactobacilli and their cell free supernatants were tested by agar spot assays and agar well diffusion assay. A total of eight

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KIECKA, ANETA, BARBARA MACURA, and MARIAN SZCZEPANIK. "Can Lactobacillus spp. Be a Factor Reducing the Risk of Miscarriage?" Polish Journal of Microbiology 70, no. 4 (2021): 431–46. http://dx.doi.org/10.33073/pjm-2021-043.

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Abstract Pregnancy loss is a common obstetric problem. Significant causes of miscarriage include genetic and epigenetic disorders of the embryo, immunological and endocrine factors, uterine malformations, improper embryo selection, and lifestyle. Perhaps a hitherto underappreciated cause of miscarriage may be an abnormal microbiota composition of the female reproductive system. Lactobacillus spp. is the most common bacteria within the reproductive tract. However, the protective role of Lactobacilli in the vagina has been well described in the literature, while it is still unknown what function

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Journal, Baghdad Science. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer

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Al-Mathkhury, Harith J. F., and Alaa' Mohammed Hasan. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.2011.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer

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Beekwilder, Jules, Daniela Marcozzi, Samuele Vecchi, et al. "Characterization of Rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus." Applied and Environmental Microbiology 75, no. 11 (2009): 3447–54. http://dx.doi.org/10.1128/aem.02675-08.

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ABSTRACT Lactobacilli are known to use plant materials as a food source. Many such materials are rich in rhamnose-containing polyphenols, and thus it can be anticipated that lactobacilli will contain rhamnosidases. Therefore, genome sequences of food-grade lactobacilli were screened for putative rhamnosidases. In the genome of Lactobacillus plantarum, two putative rhamnosidase genes (ram1 Lp and ram2 Lp) were identified, while in Lactobacillus acidophilus, one rhamnosidase gene was found (ramA La). Gene products from all three genes were produced after introduction into Escherichia coli and we

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Ahmed, Monzur Morshed, Md Mizanur Rahaman, Md Nur Hossain, et al. "Charaterization of isolated potential lactobacilli and used as probiotic food." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 2 (2016): 274–83. http://dx.doi.org/10.3329/aajbb.v1i2.61575.

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This research was carried out to isolate and identify lactobacilli from yogurt and vegetables sample from Dhaka, Bangladesh. Twenty five Lactobacillus strains were isolated and identified as Lactobacillus species based on cultural characteristics, biochemical tests, and sugar fermentation. The results showed only four among twenty five isolates were highly responded to probiotic criteria such as low pH tolerance (2.5), bile salt tolerance (2%), gastric juice tolerance (0.5% bile salt, 0.2% NaCl, 0.32% pepsin pH 2.5) in-vitro and were identified strains as Lactobacillus plantarum imy-21, Lactob

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Luneva, Albina V. "Composition, isolation and identification of the microbiome of the ceca of pheasants." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 1 (2025): 164–81. https://doi.org/10.26897/0021-342x-2025-1-164-181.

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In the research, the authors describe data on the study of the taxonomic diversity of microorganisms inhabiting the gastrointestinal tract, namely the ceca of domesticated pheasants of two species (Caucasian, Romanian). In addition, the isolation and identification of dominant representatives of the genus Lactobacillus as promising representatives of probiont strains was carried out. The composition of taxonomic groups of microorganisms in the contents of the ceca of pheasants was studied by a modern bacterial metagenomic research. Isolation of pure cultures of dominant species of the genus La

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Fangous, M. S., Y. Alexandre, N. Hymery, et al. "Lactobacilli intra-tracheal administration protects from Pseudomonas aeruginosa pulmonary infection in mice – a proof of concept." Beneficial Microbes 10, no. 8 (2019): 893–900. http://dx.doi.org/10.3920/bm2019.0069.

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The spreading of antibiotic resistance is a major public health issue, which requires alternative treatments to antibiotics. Lactobacilli have shown abilities to prevent pneumonia in clinical studies when given by oral route, certainly through the gut-lung axis involvement. Rationally, respiratory administration of lactobacilli has been developed and studied in murine model, to prevent from respiratory pathogens. It allows a direct effect of probiotics into the respiratory system. To our knowledge, no study has ever focused on the effect of probiotic intra-respiratory administration to prevent

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Foley, Matthew H., Sarah O’Flaherty, Garrison Allen, et al. "Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization." Proceedings of the National Academy of Sciences 118, no. 6 (2021): e2017709118. http://dx.doi.org/10.1073/pnas.2017709118.

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Primary bile acids (BAs) are a collection of host-synthesized metabolites that shape physiology and metabolism. BAs transit the gastrointestinal tract and are subjected to a variety of chemical transformations encoded by indigenous bacteria. The resulting microbiota-derived BA pool is a mediator of host–microbiota interactions. Bacterial bile salt hydrolases (BSHs) cleave the conjugated glycine or taurine from BAs, an essential upstream step for the production of deconjugated and secondary BAs. Probiotic lactobacilli harbor a considerable number and diversity of BSHs; however, their contributi

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Harčárová, M., E. Čonková, P. Naď, P. Váczi, and M. Proškovcová. "In Vitro Inhibitory Activity of Cell-Free Supernatants of Lactobacillus Spp. and Bacillus Spp. Against Fusarium graminearum." Folia Veterinaria 65, no. 3 (2021): 9–14. http://dx.doi.org/10.2478/fv-2021-0022.

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Abstract In this study, the antifungal activity of cell-free supernatant (CFS) of Lactobacillus spp. (Lactobacillus plantarum CCM 1904; L81, Lactobacillus fermentum; 2I3, Lactobacilus reuteri; 2/6, L26;) and Bacillus spp. (Bacillus subtilis CCM 2794, Bacillus licheniformis CCM 2206) against two strains of Fusarium graminearum CCM F-683 and Fusarium graminearum CCM 8244 were investigated in vitro. All tested CFS of Lactobacillus spp. were able to inhibit the growth of both strains of Fusarium graminearum. The highest inhibitory effect (IE) (56.5 %) against F. graminearum CCM F-683 was observed

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Rocha-Ramírez, Luz María, Ulises Hernández-Chiñas, Silvia Selene Moreno-Guerrero, Arturo Ramírez-Pacheco, and Carlos A. Eslava. "Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products." Microorganisms 9, no. 4 (2021): 825. http://dx.doi.org/10.3390/microorganisms9040825.

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Lactobacilli species are an effective biotherapeutic alternative against bacterial infections and intestinal inflammatory disorders. However, it is important to evaluate their beneficial properties, before considering them as probiotics for medical use. In this study we evaluated some probiotic properties of Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLSD, Lactobacillus helveticus IMAU70129, and Lactobacillus casei IMAU60214 previously isolated from dairy products and as control Lactobacillus casei Shirota. Experimental evaluations revealed that all strains expressed hydrophobicity (2

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Spurbeck, Rachel R., and Cindy Grove Arvidson. "Inhibition of Neisseria gonorrhoeae Epithelial Cell Interactions by Vaginal Lactobacillus Species." Infection and Immunity 76, no. 7 (2008): 3124–30. http://dx.doi.org/10.1128/iai.00101-08.

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ABSTRACT High levels of Lactobacillus, the dominant genus of the healthy human vaginal microbiota, have been epidemiologically linked to a reduced risk of infection following exposure to the sexually transmitted pathogen Neisseria gonorrhoeae. In this work, a cell culture model of gonococcal infection was adapted to examine the effects of lactobacilli on gonococcal interactions with endometrial epithelial cells in vitro. Precolonization of epithelial cells with Lactobacillus jensenii, Lactobacillus gasseri ATCC 33323, or L. gasseri ATCC 9857 reduced gonococcal adherence by nearly 50%. Lactobac

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Qin, Da, Yixuan Ma, Yanhong Wang, Xilin Hou, and Liyun Yu. "Contribution of Lactobacilli on Intestinal Mucosal Barrier and Diseases: Perspectives and Challenges of Lactobacillus casei." Life 12, no. 11 (2022): 1910. http://dx.doi.org/10.3390/life12111910.

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The intestine barrier, the front line of normal body defense, relies on its structural integrity, microbial composition and barrier immunity. The intestinal mucosal surface is continuously exposed to a complex and dynamic community of microorganisms. Although it occupies a relatively small proportion of the intestinal microbiota, Lactobacilli has been discovered to have a significant impact on the intestine tract in previous studies. It is undeniable that some Lactobacillus strains present probiotic properties through maintaining the micro-ecological balance via different mechanisms, such as m

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Terzic-Vidojevic, Amarela, Sanja Mihajlovic, Gordana Uzelac, et al. "Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows’ milk cheeses." Archives of Biological Sciences 66, no. 1 (2014): 179–92. http://dx.doi.org/10.2298/abs1401179t.

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The aim of this study was to identify and characterize the lactic acid bacteria (LAB) of artisanal Golija raw and cooked cows? milk cheeses traditionally manufactured without the addition of starter culture. A total of 188 Gram-positive and catalase-negative isolates of Golija cheeses were obtained from seven samples of different ripening time. Phenotypebased assays as well as rep-PCR and 16S rDNA sequence analysis were undertaken for all 188 Lstrains. The most diverse species were isolated from 20-day-old BGGO8 cheese (Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus casei/para

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Pedersen, Carsten, and Stefan Roos. "Lactobacillus saerimneri sp. nov., isolated from pig faeces." International Journal of Systematic and Evolutionary Microbiology 54, no. 4 (2004): 1365–68. http://dx.doi.org/10.1099/ijs.0.03057-0.

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In studying the composition of the Lactobacillus flora of faeces from pigs fed different diets, isolates with notable differences in their 16S rRNA gene sequence compared to recognized species were found. Phenotypic characteristics together with 16S rRNA gene sequences revealed that the isolates represented a novel species belonging to the Lactobacillus mali subgroup of lactobacilli. The name Lactobacillus saerimneri sp. nov. is proposed (type strain GDA154T=LMG 22087T=DSM 16049T=CCUG 48462T).

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Mokhtari, Sepideh, Saede Atarbashi-Moghadam, Elahe Motevaseli, Soudeh Ghafouri-Fard, and Ardeshir Hesampour. "Lactobacillus fermentum and Lactobacillus crispatus Do Not Have Cytotoxic Effects on HN5 Oral Squamous Cell Carcinoma Cell Line." International Journal of Dentistry 2021 (September 28, 2021): 1–6. http://dx.doi.org/10.1155/2021/3034068.

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Background. The oral environment has a very complex normal flora and a wide variety of bacteria including lactobacilli. Studies have shown oral microbial flora has important influence in the development of oral cancer. Squamous cell carcinomas account for more than 90% of cancers in oral cavity. Lactobacilli are known as one of the newest methods for the prevention and treatment of cancers. Previous studies on the effects of probiotics on oral cancer cells are very limited, and only two species of Lactobacillus which are not present in the normal oral microflora have been studied. Due to the u

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