Academic literature on the topic 'Lentiviral vector'

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Journal articles on the topic "Lentiviral vector"

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Yew, Chee-Hong Takahiro, Narmatha Gurumoorthy, Fazlina Nordin, Gee Jun Tye, Wan Safwani Wan Kamarul Zaman, Jun Jie Tan, and Min Hwei Ng. "Integrase deficient lentiviral vector: prospects for safe clinical applications." PeerJ 10 (August 12, 2022): e13704. http://dx.doi.org/10.7717/peerj.13704.

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HIV-1 derived lentiviral vector is an efficient transporter for delivering desired genetic materials into the targeted cells among many viral vectors. Genetic material transduced by lentiviral vector is integrated into the cell genome to introduce new functions, repair defective cell metabolism, and stimulate certain cell functions. Various measures have been administered in different generations of lentiviral vector systems to reduce the vector’s replicating capabilities. Despite numerous demonstrations of an excellent safety profile of integrative lentiviral vectors, the precautionary approa
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Li, Chen, Biao Qian, Zhao Ni, Qinzhang Wang, Zixiong Wang, Luping Ma, Zhili Liu, Qiang Li, and Xinmin Wang. "Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal." Open Life Sciences 15, no. 1 (March 25, 2020): 83–91. http://dx.doi.org/10.1515/biol-2020-0010.

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AbstractThis study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders. In this study, the human SCL gene was successfully cloned, and the recombinant lentivirus GV287-SCL was successfully constructed. The titer of the recombinant lentivirus was 5 × 108 TU /mL. After transfecting the ICCs with the lentiviral vector at different MOIs, the optimal MOI was determined to be 10.0, and the optimal transfection time was determined to be 3 d
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Perry, Christopher, and Andrea C. M. E. Rayat. "Lentiviral Vector Bioprocessing." Viruses 13, no. 2 (February 9, 2021): 268. http://dx.doi.org/10.3390/v13020268.

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Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian cells and are now commonly utilised within the growing field of cell and gene therapy for the treatment of monogenic diseases and adoptive therapies such as chimeric antigen T-cell (CAR-T) therapy. This is a comprehensive review of the individual bioprocess operations employed in LV production. We highlight the role of envelope proteins in vector design as well as their impact on the bioprocessing of lentiviral vectors. An overview of the current state of these operations provides opportunities for b
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Wang, Nan, Narendiran Rajasekaran, Tieying Hou, and Elizabeth Mellins. "Comparison of protein expression by different lentiviral vectors (51.12)." Journal of Immunology 188, no. 1_Supplement (May 1, 2012): 51.12. http://dx.doi.org/10.4049/jimmunol.188.supp.51.12.

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Abstract HIV1-derived lentiviral vectors have been widely used as gene delivery tools due to their potent transduction capacity and stable expression after chromosomal integration in dividing and non-dividing mammalian cells. Lentiviral vectors were screened for expressing the murine class II chaperone, invariant chain (Ii), in hematopoietic stem and progenitor cells (HSPC). We compared various lentiviral vectors using GFP as a reporter gene in 293T cells and HSPC. We assessed a dual promoter vector (DP) with separate promoters for Ii and GFP, a fusion protein vector (FU) that expresses Ii and
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Nguyen, Tuan Huy, Tatiana Khakhoulina, Andrew Simmons, Philippe Morel, and Didier Trono. "A Simple and Highly Effective Method for the Stable Transduction of Uncultured Porcine Hepatocytes Using Lentiviral Vector." Cell Transplantation 14, no. 7 (August 2005): 489–96. http://dx.doi.org/10.3727/000000005783982828.

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Gene therapy is an attractive approach for the treatment of a wide spectrum of liver diseases. Lentiviral vectors allow the stable integration of transgenes into the genome of nondividing differentiated cells including hepatocytes and could provide long-lasting expression of a therapeutic gene. To develop such approaches, preclinical studies in large animal models such as pigs are necessary to evaluate the feasibility and safety of stable lentiviral integration and long-term vector expression. In addition, effective lentivector-mediated gene transfer onto porcine hepatocytes could advance in c
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Kubo, Shuji, and Kohnosuke Mitani. "A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector." Journal of Virology 77, no. 5 (March 1, 2003): 2964–71. http://dx.doi.org/10.1128/jvi.77.5.2964-2971.2003.

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ABSTRACT To achieve efficient and sustained gene expression, we developed a new lentivirus/adenovirus hybrid vector (LA vector) that encodes sequences required for production of a human immunodeficiency virus-based lentiviral vector (i.e., a lentiviral vector, a gag/pol/rev expression cassette, a tetracycline-inducible envelope cassette, and the tetracycline-inducible transcriptional activator cassette) in a single helper-dependent adenovirus vector backbone. Via either transfection or infection, human cell lines transduced with the LA vector produced a lentiviral vector in a doxycycline-depen
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Sakuma, Toshie, Michael A. Barry, and Yasuhiro Ikeda. "Lentiviral vectors: basic to translational." Biochemical Journal 443, no. 3 (April 16, 2012): 603–18. http://dx.doi.org/10.1042/bj20120146.

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More than two decades have passed since genetically modified HIV was used for gene delivery. Through continuous improvements these early marker gene-carrying HIVs have evolved into safer and more effective lentiviral vectors. Lentiviral vectors offer several attractive properties as gene-delivery vehicles, including: (i) sustained gene delivery through stable vector integration into host genome; (ii) the capability of infecting both dividing and non-dividing cells; (iii) broad tissue tropisms, including important gene- and cell-therapy-target cell types; (iv) no expression of viral proteins af
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Breckpot, Karine, David Escors, Frederick Arce, Lucienne Lopes, Katarzyna Karwacz, Sandra Van Lint, Marleen Keyaerts, and Mary Collins. "HIV-1 Lentiviral Vector Immunogenicity Is Mediated by Toll-Like Receptor 3 (TLR3) and TLR7." Journal of Virology 84, no. 11 (March 17, 2010): 5627–36. http://dx.doi.org/10.1128/jvi.00014-10.

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ABSTRACT Lentiviral vectors are promising vaccine vector candidates that have been tested extensively in preclinical models of infectious disease and cancer immunotherapy. They are also used in gene therapy clinical trials both for the ex vivo modification of cells and for direct in vivo injection. It is therefore critical to understand the mechanism(s) by which such vectors might stimulate the immune system. We evaluated the effect of lentiviral vectors on myeloid dendritic cells (DC), the main target of lentiviral transduction following subcutaneous immunization. The activation of DC culture
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Park, Frank. "Lentiviral vectors: are they the future of animal transgenesis?" Physiological Genomics 31, no. 2 (October 2007): 159–73. http://dx.doi.org/10.1152/physiolgenomics.00069.2007.

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Lentiviral vectors have become a promising new tool for the establishment of transgenic animals and the manipulation of the mammalian genome. While conventional microinjection-based methods for transgenesis have been successful in generating small and large transgenic animals, their relatively low transgenic efficiency has opened the door for alternative approaches, including lentiviral vectors. Lentiviral vectors are an appealing tool for transgenesis in part because of their ability to incorporate into genomic DNA with high efficiency, especially in cells that are not actively dividing. Lent
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Lucke, Susann, Thomas Grunwald, and Klaus Überla. "Reduced Mobilization of Rev-Responsive Element-Deficient Lentiviral Vectors." Journal of Virology 79, no. 14 (July 2005): 9359–62. http://dx.doi.org/10.1128/jvi.79.14.9359-9362.2005.

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ABSTRACT Infection of cells transduced with a lentiviral vector by human immunodeficiency virus (HIV) could lead to packaging of the lentiviral vector RNA into HIV particles and unintended transfer of the vector. To prevent this, the Rev-responsive element (RRE) of an HIV-1 vector was functionally replaced by a heterologous RNA element (MS2). Providing Rev fused to an MS2 binding protein allowed efficient vector production. Mobilization of the vector from infected target cells was below the level of detection and at least 103- to 104-fold lower than for the RRE-containing vector. Thus, RRE-def
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Dissertations / Theses on the topic "Lentiviral vector"

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Trimby, Christopher Matthew. "STRATEGIES FOR TARGETING LENTIVIRAL VECTORS." UKnowledge, 2011. http://uknowledge.uky.edu/gradschool_diss/157.

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Lentiviral gene therapy has held great promise for treating a wide range of neurological disorders due to its ability to stably integrate into the genome of nondividing cells like neurons, in addition to dividing cells. The nervous system is a complex and highly heterogeneous system, and while a therapeutic intervention may have beneficial effects in one population of cells it may have severe side effects in another. For this reason, specific targeting of lentiviral vectors is crucial for their ultimate utility for research and clinical research use. Two different approaches for focusing the t
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Ingrao, Dina. "Etude de l'étape d'entrée des vecteurs lentiviraux dérivés du VIH-1 dans les cellules hématopoïétiques humaines." Thesis, Evry-Val d'Essonne, 2013. http://www.theses.fr/2013EVRY0021/document.

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Les vecteurs lentiviraux (LV) sont des outils efficaces de transfert de gène, largement utilisés en thérapie génique, en particulier pour la transduction ex vivo de cellules souches et progénitrices hématopoïétiques (CSPH). Afin d’étudier simultanément la fusion et la transduction dans les CSPH avec les LV, nous avons adapté une méthode basée sur latechnologie du transfert d’énergie entre deux molécules fluorescentes (FRET). Pour mettre en place cette technique, des LV capables d’incorporer spécifiquement une enzyme, la bétalactamase (BLAM-LV) et de coder une forme tronquée du récepteur au fac
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Thomas, Joan Helen. "Studies in gene transfer using pseudotyped lentiviral vector systems." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621818.

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Gelinas, Jean-Francois. "Enhancement of lentiviral vector production through alteration of virus-cell interactions." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:9921b8b4-e2b5-4eec-9efc-6036765c8d55.

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Gene therapy is the introduction or alteration of genetic material with the intention to treat disease. To support this aim, viruses have been modified, with elements linked to viral pathogenicity removed from their genome and replaced by the genetic material to be delivered. Gene therapy vectors based on lentiviruses have many advantages, such as the ability to transduce non-dividing cells and to target specific cell types via pseudotyping. They have been successfully used in ex vivo clinical trials for several haematopoietic stem cell disorders. Lentiviral vectors, however, suffer from subst
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Zhang, Bing. "Lentiviral vector-mediated gene transfer in vitro and in vivo /." St. Lucia, Qld, 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18024.pdf.

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Booth, C. A. "Lentiviral vector mediated gene therapy for X-linked lymphoproliferative disease." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1356299/.

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X-linked lymphoproliferative disease (XLP) is a rare primary immunodeficiency characterised by severe immune dysregulation and is caused by mutations in the SH2D1A gene. Clinical manifestations vary and include haemophagocytic lymphohistiocytosis (HLH), lymphoma and dysgammaglobulinaemia, often triggered by Epstein-Barr virus (EBV) infection. SLAM-associated protein (SAP) is a key regulator of immune function in T, NK, and NKT cells and defects in this protein lead to the cellular and humoral immune defects described in patients. Treatment options for XLP are limited and currently haematopoiet
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Macdonald, D. "Lentiviral vector vaccines for T-cell-mediated protection against influenza." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1417882/.

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Vaccines that induce T cells which recognize conserved viral proteins could confer cross-strain protection against pathogens with fast-mutating B cell epitopes. Influenza is an example of such a pathogen for which there is a pressing need for a universal vaccine. Lentiviral vectors are a counterintuitive choice as vaccines since they have low inherent immunogenicity. However, their efficient transduction of non-dividing cells and high capacity permits transduction of antigen presenting cells with not only antigen but also molecular adjuvants that directly or indirectly enhance the T cell respo
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Oakland, Mayumi. "Improving lentiviral vector-mediated gene transfer by understanding cellular barriers." Diss., University of Iowa, 2013. https://ir.uiowa.edu/etd/4709.

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Cystic fibrosis (CF) is an autosomal recessive genetic disorder of which lung disease is the leading cause of morbidity and mortality. One attractive strategy for the treatment of CF lung disease is to directly deliver CF transmembrane conductance regulator gene to airway epithelia. Although promising results have been reported, barriers present in the lung make successful gene transfer to the respiratory tract difficult. In order to improve gene transfer strategies in the intrapulmonary airways, we need to identify the bottlenecks of transduction for the vector system. A previous study report
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Mekkaoui, Leila. "Lentiviral vector purification using genetically encoded biotin mimic in packaging cell." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10053191/.

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Lentiviral vectors (LVs) are powerful tools in gene therapy that have recently witnessed an increasing demand in both research and clinical applications. Current LVs purification represents the main bottle neck in their application as several methods are employed which are time consuming, cumbersome and yield low recoveries. The aim of this project was to develop a one-step method to specifically and efficiently purify LVs, with high vector yields and reduced levels of impurities, using the biotin-streptavidin system. Herein, packaging 293T cells were genetically engineered with biotin mimicki
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FIRRITO, CLAUDIA. "Targeted Gene Correction and Reprogramming of SCID-X1 Fibroblasts to Rescue IL2RG Expression in iPSC-derived Hematopoietic Cells." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/94656.

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La terapia genica basata sull’utilizzo di vettori integranti è stata già applicata con successo per la cura di varie malattie genetiche come le malattie da accumulo lisosomiale (LSD), la beta-talassemia (β-Thal) e le immunodeficienze primarie (PID). L’immunodeficienza combinata grave legata al cromosoma X (SCID-X1) è una malattia monogenica letale causata da mutazioni del gene codificante la catena comune gamma del recettore per l’interleuchina 2 (IL2RG). I primi studi clinici per la SCID-X1 hanno mostrato il potenziale terapeutico della terapia genica basata su vettori integranti, risultando
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Books on the topic "Lentiviral vector"

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Lentiviral vector systems for gene transfer. Georgetown, TX: Eurekah.com, 2003.

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Trono, Didier, ed. Lentiviral Vectors. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56114-6.

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Escors, David, Karine Breckpot, Frederick Arce, Grazyna Kochan, and Holly Stephenson. Lentiviral Vectors and Gene Therapy. Basel: Springer Basel, 2012. http://dx.doi.org/10.1007/978-3-0348-0402-8.

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Escors, David. Lentiviral Vectors and Gene Therapy. Basel: Springer Basel, 2012.

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Maurizio, Federico, ed. Lentivirus gene engineering protocols. Totowa, N.J: Humana Press, 2003.

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Federico, Maurizio, ed. Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3753-0.

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Maurizio, Federico, ed. Lentivirus gene engineering protocols. 2nd ed. New York: Humana Press, 2010.

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Lentiviral Vector Systems for Gene Transfer (Medical Intelligence Unit, 31). Springer, 2003.

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Lentiviral Vector Systems for Gene Transfer (Medical Intelligence Unit, 31). Eurekah.com, 2001.

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Buchschacher, Gary L. Lentiviral Vector Systems for Gene Transfer (Medical Intelligence Unit, 31). Eurekah.com, 2001.

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Book chapters on the topic "Lentiviral vector"

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Larochelle, A., K. W. Peng, and S. J. Russell. "Lentiviral Vector Targeting." In Current Topics in Microbiology and Immunology, 143–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56114-6_7.

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Segura, María Mercedes, Alain Garnier, Yves Durocher, Sven Ansorge, and Amine Kamen. "New Protocol for Lentiviral Vector Mass Production." In Lentivirus Gene Engineering Protocols, 39–52. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_2.

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Ramezani, Ali, and Robert G. Hawley. "Strategies to Insulate Lentiviral Vector-Expressed Transgenes." In Lentivirus Gene Engineering Protocols, 77–100. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_5.

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Han, Shuhong, and Lung-Ji Chang. "Immunity of Lentiviral Vector-Modified Dendritic Cells." In Gene Therapy of Cancer, 245–59. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-561-9_13.

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Chong, Mark S. K., and Jerry Chan. "Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells." In Lentivirus Gene Engineering Protocols, 135–47. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_9.

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Cui, Yan, and Lung-Ji Chang. "Detection and Selection of Lentiviral Vector-Transduced Cells." In Lentivirus Gene Engineering Protocols, 69–85. Totowa, NJ: Humana Press, 2003. http://dx.doi.org/10.1385/1-59259-393-3:69.

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Scherr, Michaela, Letizia Venturini, and Matthias Eder. "Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs." In Lentivirus Gene Engineering Protocols, 175–85. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_12.

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ter Brake, Olivier, Jan-Tinus Westerink, and Ben Berkhout. "Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy." In Lentivirus Gene Engineering Protocols, 201–13. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_14.

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Du, Zhong-Wei, and Su-Chun Zhang. "Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells." In Lentivirus Gene Engineering Protocols, 127–34. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-533-0_8.

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Verhoeyen, Els, Caroline Costa, and Francois-Loic Cosset. "Lentiviral Vector Gene Transfer into Human T Cells." In Genetic Modification of Hematopoietic Stem Cells, 97–114. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-409-4_8.

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Conference papers on the topic "Lentiviral vector"

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Ranzani, Marco, Daniela Cesana, Cynthia C. Bartholomä, Francesca Sanvito, Michela Riba, Mauro Pala, Fabrizio Benedicenti, et al. "Abstract 3169: Lentiviral vector-based insertional mutagenesis identifies new clinically relevant liver cancer genes." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3169.

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Albershardt, Tina C., David J. Campbell, Andrea J. Parsons, Jan H. ter Meulen, and Peter Berglund. "Abstract 2506: Preclinical characterization of LV305, a lentiviral vector targeting tumors expressing NY-ESO-1." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-2506.

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Dwivedi, Alka, Ling Su, Justin Mirazee, Mehdi Benzaoui, Christopher Chien, Nirali Shah, Xiaolin Wu, and Naomi Taylor. "327 Clonal expansion of CD22 CAR T-cells following lentiviral vector integration in the PWWP3A gene." In SITC 37th Annual Meeting (SITC 2022) Abstracts. BMJ Publishing Group Ltd, 2022. http://dx.doi.org/10.1136/jitc-2022-sitc2022.0327.

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Qin, Shi, and Jie Wang. "The construction of RGD-TAT-KDR siRNA fusion gene lentiviral vector and the study of its antitumor activityin vitro." In International Conference on Medical Engineering and Bioinformatics. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/meb140171.

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Bajaj, Anshika, Tsai-Yu Lin, Lisa Y. Ngo, Michele Murphy, Brenna Kelley-Clarke, Wayne R. Gombotz, Jan H. ter Meulen, and Peter Berglund. "Abstract 5919: Component-specific qPCR assays for characterization and identity testing of multigenome ZVex®, a dendritic cell-targeting lentiviral vector platform." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-5919.

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Bajaj, Anshika, Lisa Y. Ngo, Peter Berglund, and Jan ter Meulen. "Abstract 5092: ZVex® lentiviral vector strongly activates pro-inflammatory, antigen processing, and anti-viral defense response pathways in monocyte-derived dendritic cells." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-5092.

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Ranzani, Marco, Daniela Cesana, Cynthia Bartholomae, Francesca Sanvito, Mauro Pala, Fabrizio Benedicenti, Lucia Sergi Sergi, et al. "Abstract 4982: Identification of new human liver cancer genes by a novel lentiviral vector-based insertional mutagenesis approach in three mouse models of hepatocarcinogenesis." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-4982.

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Ranzani, Marco, Daniela Cesana, Cynthia C. Bartholomae, Francesca Sanvito, Mauro Pala, Fabrizio Benedicenti, Pierangela Gallina, et al. "Abstract 104: New liver cancer genes identified by lentiviral vector-based insertional mutagenesis in mice are associated to differential survival in hepatocellular carcinoma patients." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-104.

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Bryson, Paul D., Xiaolu Han, Norman Truong та Pin Wang. "Abstract 2888: Dendritic cell-targeted lentiviral vector vaccines overcome tolerance to generate a protective T-cell immune response to breast cancer antigens ERBB2 and α-lactalbumin". У Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-2888.

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Nicolai, Chris, Jim Qin, Way Wu, Mollie McDonnell, Erica Shirazi, Greyson Hamilton, Max Chen, et al. "1230 VivoVec lentiviral vector particles surface-engineered with T cell activating and co-stimulatory ligands enhancein vivoCAR T cell generation and antitumor activity." In SITC 37th Annual Meeting (SITC 2022) Abstracts. BMJ Publishing Group Ltd, 2022. http://dx.doi.org/10.1136/jitc-2022-sitc2022.1230.

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Reports on the topic "Lentiviral vector"

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Yes, Jiing-Kuan. Anti-Angiogenic Gene Therapy of Prostate Cancer with Lentiviral Vectors. Fort Belvoir, VA: Defense Technical Information Center, July 2004. http://dx.doi.org/10.21236/ada428533.

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Yee, Jiing-Kuan. Anti-Angiogenic Gene Therapy of Prostate Cancer With Lentiviral Vectors. Fort Belvoir, VA: Defense Technical Information Center, July 2002. http://dx.doi.org/10.21236/ada410315.

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Yee, Jiing-Kuan. Anti-Angiogenic Gene Therapy of Prostate Cancer with Lentiviral Vectors. Fort Belvoir, VA: Defense Technical Information Center, July 2003. http://dx.doi.org/10.21236/ada418264.

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