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Dissertations / Theses on the topic 'Live sperm'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

SUGANUMA, NOBUHIKO, YOSHIMASA ASADA, YUTAKA TOMODA, ATSUO ITAKURA, and MASANORI YAMAMOTO. "A Live Birth from Intracytoplasmic Injection of a Testicular Spermatozoon." Nagoya University School of Medicine, 1997. http://hdl.handle.net/2237/16749.

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Raheem-Kizchery, Ruby. "Assessment of DNA degradation in live spermatozoon using laser tweezers Raman microspectrometry." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/25706.

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Purpose: Sperm nuclear proteins and DNA integrity have been implicated in infertility and treatment failures. High stallion to stallion variability is observed in sperm cryopreservation protocols. The cells are destroyed with harsh chemicals prior to using biochemical assays to test sperm DNA quality. The feasibility of using Raman spectrometry in combination with a laser trap for non-destructive micromanipulation and characterization of DNA damage in motile stallion and human sperm is experimentally investigated in this thesis. Methods: Live stallion sperms were subjected to controlled cellul

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3

Paul, Catriona. "The origins and consequences of DNA damage in the male germ line." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/3443.

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Infertility affects ~20% of couples in Europe and in 50% of cases the problem lies with the male. The development of assisted reproductive technologies (ART) such as in vitro fertilisation (IVF) and intra-cytoplasmic spermatozoa injection (ICSI) has allowed some couples to overcome male-factor infertility. However concerns remain over the increasing use of ART as elevated levels of DNA damage in sperm from infertile men have been reported and a link between DNA damage in sperm and early embryonic failure has been demonstrated. DNA damage in sperm, caused by oxidative stress may also be passed

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Brodin, Thomas. "Ovarian Reserve and Assisted Reproduction." Doctoral thesis, Uppsala universitet, Obstetrik & gynekologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-192998.

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Treatment success in IVF-ICSI is mainly limited by female age, but differences in ovarian reserve (OR; the remaining pool of oocytes and their quality) between individuals modify treatment prerequisites among women of similar age. OR may be assessed by OR tests (ORTs). The main aims of this work were to study menstrual cycle length (MCL), basal levels of circulating gonadotrophins, antral follicle count (AFC) and serum Anti-Müllerian hormone (AMH) levels and their associations with and prognostic capacities regarding IVF-ICSI outcome in large cohorts of unselected women. Age-adjusted MCL was p

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Brazdova, Andrea. "Study of immunological properties of sperm and seminal plasma antigens : anti-seminal and anti-sperm antibodies in female immune infertility : characterization of targeted proteins." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2014. http://tel.archives-ouvertes.fr/tel-01037917.

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The World health Organization reports infertility as a disease and a failure of reproductive tract to achieve pregnancy after 12 months or more of regular unprotected sexual intercourse. Nowadays, infertility has become a common life phenomenon affecting 1 out of 5 couples at reproductive age. Idiopathic cause is mostly associated with active immune system which may produce high levels of anti-seminal and/or anti-sperm antibodies. Auto-immunization as well as iso-immunization has a significant role in up to 30% of reported cases of infertility. Semen that is defined as a complex fluid containi

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Santoso, Benedicta. "The effect of sperm apoptosis, Reactive Oxygen Species (ROS) and semen sample handling on sperm parameters and Assisted Reproductive Technology (ART) outcomes." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2020. https://ro.ecu.edu.au/theses/2373.

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Overall, this doctoral study aimed to contribute to the existing knowledge and clinical practice regarding male infertility, especially in relation to the potential sources and clinical impact of sperm apoptosis and reactive oxygen species (ROS) in the context of assisted reproductive technology (ART). This thesis encompasses three independent studies, each of which addresses a single aim related to different aspects of male infertility and ART practices. Study 1 examined the relationship between sperm apoptosis and ROS levels and ART outcomes. On the day of oocyte retrieval, the levels of spe

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7

Gies, Sabrina [Verfasser]. "New molecular insights into sperm epigenetics: regulation of LINE-1 elements by CpG methylation and histone-modifications, and its impacts on sperm quality / Sabrina Elisabeth Gies." Gießen : Universitätsbibliothek, 2019. http://d-nb.info/1209134799/34.

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Gies, Sabrina Elisabeth [Verfasser]. "New molecular insights into sperm epigenetics: regulation of LINE-1 elements by CpG methylation and histone-modifications, and its impacts on sperm quality / Sabrina Elisabeth Gies." Gießen : Universitätsbibliothek, 2019. http://d-nb.info/1209134799/34.

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Robinson, Callum Andrew. "The cryopreservation of human semen, and subsequent evaluation of a commercially-available device to isolate motile sperm." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2018. https://ro.ecu.edu.au/theses/2146.

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The cryopreservation of human semen is a vital asset in assisted reproductive technologies (ART). Although advances have been in the freezing of sperm, further refinement is both necessary and ongoing. Computer-assisted semen analysis (CASA) has been increasingly utilised in both research and diagnostic however there are a range of variables that must first be controlled in order to produce reliable measurements. Following thawing, sperm must be isolated from both the original seminal plasma and the cryoprotectants; the two most used isolation methods include density gradient centrifugation (D

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Larsdotter, Mellström Helena. "Life history evolution in a bivoltine butterfly." Doctoral thesis, Stockholms universitet, Zoologiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-81399.

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Evolution is not always straight-forward, as selection pressures may differ between different generations of the same species. This thesis focuses on the evolution of life history of the model species, the Green-veined White butterfly Pieris napi. In central Sweden P. napi has two generations per year. The directly developing summer generation is short-lived and time stressed, compared to the diapausing generation. In paper I polyandry, defined as female mating rate, was shown to differ between generations but was unaffected by environmental factors. In paper II both males and females of the d

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11

Marceta, Tihana. "Assessing the impacts of seawater acidification and emerging contaminants on different life stages of the sea urchin Paracentrotus lividus." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3422674.

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Ocean acidification, originated by rising atmospheric CO2 levels, represents one of the most important future threats in marine coastal areas. Several studies have provided data about the effects of low pH on single life stages in marine invertebrate species. Presently, there is an increasing effort to produce knowledge about responses from the entire life cycle of the organisms and across generations as well. This approach has the advantage of highlighting potential phenotypic plasticity and producing more realistic data at population level. Taking this into consideration, adult sea urchins

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12

au, k. bryant@murdoch edu, and Kate Alexandra Bryant. "The mating system and reproduction in the honey possum, Tarsipes rostratus: a life-history and genetical perspective." Murdoch University, 2004. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050505.140613.

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The honey possum Tarsipes rostratus, a marsupial endemic to South-Western Australia, feeds exclusively upon nectar and pollen. It is one of the smallest marsupials, with adult females (8-12g) significantly larger than adult males (6-9g). Honey possum males have the longest sperm (356µm) recorded for any mammaland the testes represent 4.2% body weight, amongst the largest recorded formammal species. These features suggest that sperm competition is an importantpart of the mating system. This study used a combination of field based studies,DNA analysis and histological examination of the female r

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Di, Nisio Andrea. "Evolutionary consequences of producing competitive ejaculates: insights from an artificial selection study for sperm production on Poecilia reticulata." Doctoral thesis, Università degli studi di Padova, 2014. http://hdl.handle.net/11577/3424544.

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The aim of my study was to investigate the maintenance of variability for sperm production in the guppy, P. reticulata. Sperm number, which is the main determinant of SC outcome in the Trinidadian guppy population studied in my lab, shows high additive genetic variance despite strong directional selection and very high sire heritability and stronger condition dependence than other ejaculate traits; furthermore, multiply mated females produce higher quality offspring, suggesting that sperm c ompetition, and hence sperm number, may mediate genetic benefits to the female. To clarify the evolution

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Ziege, Madlen, Kristin Mahlow, Carmen Hennige-Sulz, et al. "Audience effects in the Atlantic molly (Poecilia mexicana) : prudent male mate choice in response to perceived sperm competition risk?" Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4517/.

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Background: Multidirectional interactions in social networks can have a profound effect on mate choice behavior; e.g., Poecilia mexicana males show weaker expression of mating preferences when being observed by a rival. This may be an adaptation to reduce sperm competition risk, which arises because commonly preferred female phenotypes will receive attention also from surrounding males, and/or because other males can copy the focal male's mate choice. Do P. mexicana males indeed respond to perceived sperm competition risk? We gave males a choice between two females and repeated the tests unde

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15

Neumann, Giovano. "Computer Assisted Sperm Analysis (CASA) em software livre empregado em análises espermáticas de peixes: cientometria e aplicação em rotina de reprodução artificial." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/1939.

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Made available in DSpace on 2017-07-10T18:13:17Z (GMT). No. of bitstreams: 1 Giovano Neumann.pdf: 1483055 bytes, checksum: 73310de09daeb1b45d1ac1eda6a64ce0 (MD5) Previous issue date: 2015-02-27<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>This dissertation is based on the development of two separate scientific papers. (1) First article it is a scientometric analysis: In 2007 was released in the scientific means by Wilson-Leedy and Ingermann, the first article using the CASA free software in sperm mobility analysis for fish, for this study, from of scientometric technique

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16

Maceda, Tintaya Edwin Eddy. "Effect of three cryoconservation diluents on sperm motility and vitality in the ejaculate of bulbourethal-ectomized llamas (Lama glama), department of La Paz." BYU ScholarsArchive, 2008. https://scholarsarchive.byu.edu/etd/5391.

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The purpose of this study was to evaluate the effects of three different diluents used during the cryopreservation process on the motility and vitality of sperm cells. The three diluters used in this study were: A) trice-serum-egg yolk-glycerin, B) serum-egg yolk-glycerin, and C) Dulbecco’s serum-egg-yolk- glycerin. Diluters were tested in proportions of 64-15-15-6% (N1), 54-20-20-6% (N2), and 44-35-15-6% (N3). Llama semen was collected at the Mejoramiento Genético y Diagnóstico Clínico Del Servicio Agropecuario (SEDAG) in the Los Andes Province of the Department of La Paz. The procedure took

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17

Parlayan, Filiz Eanes Ritchie. "Development of inductively coupled plasma spectroscopic methods for the determination of metals in beluga (delphinapterus leucas) and pygmy sperm (kogia breviceps) whale liver samples/." [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/kimya/t000399.pdf.

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Thesis (Master)--İzmir Institute of Technology, İzmir, 2005<br>Keywords: ICP-OES, Chemometrics methods, internal standardization, matrix effects. Includes bibliographical references (leaves. 111-120).

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18

Maj, El sharif. "Early life stress and its association with epigenetics and immune system response." Thesis, Linköpings universitet, Biologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-138947.

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Stress can induce prolonged deleterious effects on many characteristics in chickens (Gallus gallus). Particular interest has been paid to early life stress. Social isolation as an early life stressor results in increased plasma corticosterone levels. Moreover, it induces behavioural and physiological changes as well as gene expression modifications in the hypothalamus. In the first part of my study, I aim to inquire into social isolation impacts on the short and long-term. Short and long-term effects were assessed by immune system, behaviour and weight. 82 male chickens were assigned to three

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19

Behrens, Verena. "Die Rolle des Glucosetransporters 8 (Slc2a8) in der Regulation der Glucosehomöostase, der Spermienmotilität sowie des Verhaltens." Phd thesis, Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2009/3630/.

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Der ubiquitär exprimierte, multifunktionale Glucosetransporter GLUT8 gehört zur Klasse III der Familie der passiven Glucosetransporter, die aus insgesamt 14 Proteinen besteht. Die fünf Mitglieder der Klasse IIII unterscheiden sich strukturell leicht von den Mitgliedern der Klasse I und II (Joost und Thorens, 2001). GLUT8 besitzt ein N-terminales Dileucin-Motiv, das Teil eines [DE]XXXL[LI] Motivs ist, welches für die Sortierung des Transporters in späte Endosomen und Lysosomen verantwortlich ist (Augustin et al., 2005). Da bis heute kein Signal identifiziert wurde, das eine Translokation des Tr

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20

Fernández, Álvarez Fernando Ángel. "An onto-phylogenetic journey through the life history of flying squids (Cephalopoda: Ommastrephidae)." Doctoral thesis, Universitat Politècnica de Catalunya, 2018. http://hdl.handle.net/10803/606428.

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Flying squids develop all its life cycle in the water column, as planktonic paralarvae and then as nektonic subadults and adults. In this Ph. D. Thesis, light was shed over several poorly understood aspects of the ontogeny and phylogeny of the Family Ommastrephidae. The mechanism of sperm migration from spermatangia to the female seminal receptacles was studied. Spermatozoa are able to actively migrate between both structures. The morphology of the hatchling of three Mediterranean ommastrephid species was studied based on embryos obtained by in vitro fertilization and a dichotomous key was dev

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Abaurrea, Velasco Clara [Verfasser], Gerhard [Gutachter] Gompper, Matthias [Gutachter] Sperl, and Ulrich [Gutachter] Schwarz. "Active matter: from collective self-propelled rods to cell-like particles / Clara Abaurrea Velasco ; Gutachter: Gerhard Gompper, Matthias Sperl, Ulrich Schwarz." Köln : Universitäts- und Stadtbibliothek Köln, 2018. http://d-nb.info/1168245885/34.

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22

Pereira, Eliana Beatriz. "Uma infraestrutura para consist?ncia dos processos de software baseados no metamodelo SPEM 2.0." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2011. http://tede2.pucrs.br/tede2/handle/tede/5202.

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Made available in DSpace on 2015-04-14T14:49:57Z (GMT). No. of bitstreams: 1 444969.pdf: 8863468 bytes, checksum: d6f97c49279c5546c9619f8479b0b679 (MD5) Previous issue date: 2011-06-27<br>The use of software development processes in the IT organizations has become common. This happens because the quality product is related to the process quality. The main interest of the IT companies is to adopt one or more well-defined software development processes and tailor them when necessary to meet the projects specific needs. However, since the amount of elements and relationships of a software devel

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Holembowski, Lena [Verfasser], Matthias [Akademischer Betreuer] Dobbelstein, and Michael [Akademischer Betreuer] Kessel. "The p53 homolog p73 takes hold of the male germ line – a novel function of TAp73 in protecting sperm cell adhesion, migration and maturation within the seminiferous epithelium of the testis / Lena Holembowski. Gutachter: Matthias Dobbelstein ; Michael Kessel. Betreuer: Matthias Dobbelstein." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://d-nb.info/1044871377/34.

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24

Romdhane, Samira. "Les nouvelles technologies de l'assistance médicale à la procréation (amp) et la qualité des gamètes et des embryons : évaluation de l'épigénome." Phd thesis, Université Claude Bernard - Lyon I, 2010. http://tel.archives-ouvertes.fr/tel-00838798.

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Les techniques d'assistance médicale à la procréation particulièrement l'induction de l'ovulation, la maturation in vitro des ovocytes et la culture embryonnaire prolongée impliquent la manipulation des gamètes ainsi que les embryons à des moments critiques de leur maturation et développement qui sont également des étapes clé du remodelage épigénétique. Par conséquent, elles pourraient interférer avec la reprogrammation épigénétique, en particulier la mise en place de la méthylation des gènes soumis a empreinte au cours de l'ovogenèse, ou son maintien au cours du développement préimplantatoire

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Noblanc, Anaïs. "Contrôle des dommages oxydants au noyau spermatique : Apports des modèles murins knock-out pour des glutathion peroxydases." Phd thesis, Université Blaise Pascal - Clermont-Ferrand II, 2013. http://tel.archives-ouvertes.fr/tel-00910253.

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Les spermatozoïdes acquièrent leur pouvoir fécondant et leur mobilité lors de leur transit le long de l'épididyme. Paradoxalement, cette maturation épididymaire nécessite la présence d'espèces oxygénées réactives (EOR) pour condenser la chromatine spermatique afin de mieux protéger l'ADN de ces mêmes molécules. Nous avons étudié la façon dont l'épididyme parvient à assurer l'équilibre entre un déficit et un excès d'EOR en caractérisant le phénotype épididymaire de souris dont la production de deux enzymes antioxydantes glutathion peroxydases a été invalidée, GPx5 et snGPx4. L'épididyme de ces

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Houle, Emily. "The Association Between Sperm DNA Methylation and Sperm Mitochondrial DNA Copy Number." 2020. https://scholarworks.umass.edu/masters_theses_2/950.

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Background: Infertility has become a growing concern across the world as cases continue to increase each year. Research has now shifted to identifying novel biomarkers to predict male fertility. While mtDNAcn has recently been found to show promising results as potential biomarker, its regulation remains unclear. Method: Triplex probe-based PCR was used to quantify mtDNA levels, while 850K Array was used to measure methylation levels. A-clustering algorithm followed by generalized estimating equations (GEE) lead to clustering of individual CpG sites, containing a minimum of 2 CpGs within 1000

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27

Evans, Karen J. (Karen Jennifer). "The life history of sperm whales, Physeter macrocephalus, from southern Australian waters." Thesis, 2003. https://eprints.utas.edu.au/19772/7/whole_EvansKarenJennifer2003_thesis_ex_pub_mat.pdf.

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One hundred and fifteen sperm whales (97 female, 15 male, 3 unknown gender) were involved in three mass stranding events during the month of February 1998 along the west and north-west coastlines of Tasmania, Australia. Sixty six of these whales stranded at Ocean Beach, Strahan, 35 at Greens Beach, Marrawah and 11 at Black River Beach, Stanley. The remaining three whales stranded singly along the coastline. Three mass strandings of this species in such close temporal proximity have not been reported in this area before, and this is the first time that samples have been systematically col

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Barzideh, Jaleh. "Epigenetic modification in human male germ line." Thesis, 2010. http://hdl.handle.net/1959.13/923302.

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Masters Research - Master of Philosophy (MPhil)<br>The purpose of this study was to examine the methylation status of human sperm DNA in relation to the functional competence of these cells. In order to achieve this aim discontinuous Percoll gradient density centrifugation was used to generate sperm populations that were either normal (high-density Percoll fraction) or functionally impaired (low density Percoll fraction). The methylation status of these cells was then examined using HPLC, immunocytochemistry or flow cytometry and ultimately correlated with additional markers reflecting the ten

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"Apoptosis-like changes in bull sperm and their effects on fertility." Thesis, 2014. http://hdl.handle.net/10388/ETD-2014-05-1545.

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The overall objective of this thesis was to evaluate the effects of apoptosis-like membrane and DNA changes in bull sperm, and to relate these changes to a bull’s fertility potential. This thesis hypothesis is that apoptosis-like changes occurring in fresh or cryopreserved bull sperm have a negative effect on a bull’s fertility potential. Two studies were conducted, the objectives of study 1 were to confirm the relationship of apoptosis-related membrane and nuclear changes in bull sperm with fertility, to predict the fertility of beef bulls used for natural mating; and to evaluate the effect

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Zheng, Heping. "Crystal structure of sperm lysozyme-like protein 1 (SLLP1) and databases for structural-functional studies." 2009. http://wwwlib.umi.com/dissertations/fullcit/3393543.

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Werner, Anne M. "Computer simulation of the underwater detection of sperm shales using modifications of line transect theory." Thesis, 1993. http://hdl.handle.net/10945/24131.

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Correia, Sara Carina de Lima. "Estrogens and regucalcin in testicular apoptosis and sperm function: “a matter of life and death"." Doctoral thesis, 2014. http://hdl.handle.net/10400.6/3987.

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Spermatogenesis is the intricate and coordinated process by which thousands of spermatozoa are produced daily within the male gonad. In addition, mammalian testis also serves as an endocrine organ, producing the sex steroid hormones needed for normal spermatogenesis and development of male phenotype. Over the years, estrogens have emerged as important regulators of male reproductive function, but their role in spermatogenesis has remained a matter of controversy. There are reports indicating that estrogens are survival factors for germ cells, while other strong evidences associated their

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Bašus, Kryštof. "Monitorování dynamiky proteinových sítí: role FcRL proteinů při interakci membrány spermie a vajíčka." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-435880.

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Sperm--egg membrane interaction and fusion is mediated by various molecules of the different protein network that are located on both egg and sperm membrane. So far, many proteins have been selected to be fusion candidates, some of them (Izumo1, CD9, Juno) were proven to be essential, whereas others were discovered to play an unsuspected new active role (CD46, tetraspanins). After the adhesion of sperm to an egg, Juno located on the oolema associates with monomeric Izumo1 on sperm me

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Holembowski, Lena. "The p53 homolog p73 takes hold of the male germ line – a novel function of TAp73 in protecting sperm cell adhesion, migration and maturation within the seminiferous epithelium of the testis." Doctoral thesis, 2012. http://hdl.handle.net/11858/00-1735-0000-0001-BC61-1.

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Die Transkriptionsfaktoren der p53 Familie besitzen diverse Aufgaben sowohl während der Tumorentstehung als auch während der Entwicklung. In seiner ursprünglichen, evolutionär konservierten Rolle hat p63, ein Mitglied der p53 Familie, die Aufgabe die genetische Stabilität von Keimzellen zu gewährleisten und beeinflusst zudem die Keimbahnentwicklung. Mit Hilfe von „knockout“ (KO) Mausmodellen wurde bereits der Einfluss der 3 Transkriptionsfaktoren p53, p63 und p73 auf die weibliche und männliche Keimbahn untersucht. Während p53KO und p63KO Mäuse fruchtbar sind, führt der gemeinsame Verlust alle

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