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Journal articles on the topic 'Live sperm'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Cho Ping, Ng, Noor Hashida Hashim, and Durriyyah Sharifah Hasan Adli. "Effects ofNigella sativa(Habbatus sauda) Oil and Nicotine Chronic Treatments on Sperm Parameters and Testis Histological Features of Rats." Evidence-Based Complementary and Alternative Medicine 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/218293.

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Twenty-fourSprague-Dawleymale rats (7–9 weeks old, 200–250 g) were divided into Nicotine (N) (0.5 mg/100 g body weight (BW), Nicotine Control (NC) (saline, 0.1 mL/100 g BW),Habbatus saudaoil (HS) (6.0 μL/100 g BW), andHabbatus saudaControl (HSC) (corn oil, 0.1 mL/100 g BW) groups and treated for 100 days. Sperm parameters and seminiferous tubules measurements were evaluated. The N showed a significantly lower sperm motility (1.03±0.05×106 sperm/mL) and percentage of normal (82.61±0.03%) and live (93.88±0.01%) sperm, higher value for the seminiferous tubule (253.36±1.83 μm) and lumen (100.15±2.

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Al-Dulimi, Ayman Ali H., and Ali Abdullah Zuairi Al-Sadoon. "Effect of Aqueous Extract of Dill leaves (AEDL) on some Traits of Ram Epididymal Sperms under Cooling Conditions." IOP Conference Series: Earth and Environmental Science 1262, no. 7 (2023): 072048. http://dx.doi.org/10.1088/1755-1315/1262/7/072048.

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Abstract The current study was conducted in the Graduate Studies Laboratory of the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of dill leaves ( AEDL)on some properties of epididymal sperms of rams under cooling conditions of 5 °C for different periods. The aqueous extract of dill leaves was prepared and kept in the refrigerator until the time of use. The epididymal sperms were obtained from the testis of Rams that slaughters in the local slaughterhouse of Al-Muthanna Governorate, the testic

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3

Mohamed, Amal, Aza Hassan, Magdy Amer, and El-Said Abdel-Aziz. "The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits." Mansoura Veterinary Medical Journal 21, no. 2 (2020): 32–38. http://dx.doi.org/10.35943/mvmj.2020.21.2.0203.

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Objectives: To investigate possible side effects of ketoconazole and griseofulvin on fertility of clinically healthy male rabbits. Design: Randomized controlled experimental study. Animals: Thirty mature male rabbits. Procedures: Rabbits were randomly allocated into three equal sized groups (10 animals each). The first group served as a control group (received no treatment), the second group received griseofulvin (25 mg/kg B.W) orally for 30 days, and the third group received ketoconazole (50 mg/kg B.W) orally for 30 days. Semen samples were collected after 1 day, 1 week and 2 weeks post-admin

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Al-Sadoon, Ali. "Effect of Aqueous extract of Moringa Olivera leaves( AEMOL) on some characteristics of ram epididymal sperms under cooling conditions." Muthanna Journal for Agricultural Sciences 10, no. 2 (2023): 1–6. http://dx.doi.org/10.52113/mjas04/10.2/12.

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The current study was conducted in the Graduate Studies Laboratory at the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of Moringa leaves on some characteristics of epididymal sperm of rams under cooling conditions for different periods. The aqueous extract of Moringa leaves was prepared and stored in the refrigerator ( 5 °C) until the time of use, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tri

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5

ROTHMAN, CAPPY MILES. "Live Sperm, Dead Bodies." Journal of Andrology 20, no. 4 (1999): 456–57. http://dx.doi.org/10.1002/j.1939-4640.1999.tb02542.x.

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6

Th. Taha, N., T. W. AL Doori, D. J. Howaizi, S. B.Al-Deen, and S. Y. Taha. "EFFECT OF STRAIN AND TERPERATURE ON SOME SEMENAL CHACTERISTICS OF LOCAL TURKEY MALRS." Iraqi Journal of Veterinary Medicine 13, no. 1 (1989): 208–17. http://dx.doi.org/10.30539/ijvm.v13i1.1672.

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This study was conducted at the turkey farm of the College of Agriculture, Salahaddin University, by using (7) black and (10) red local turkey males to study the effect of strain and environmental temperature on some semen characteristics (Semen volume, colour, pH, Individual and mass sperm motility, live and abmormal sperm percentage, and aperm concentration in cu. ml. and in ejaculate, and to study the relation between paire of characters under study. Analysis of voriance showed that strain had a signifiant effect (p<0.05) only on mass sperm motility and sperm concentration in cu ml trait

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7

Reynolds, Steven, Sarah J. Calvert, Stephen J. Walters, Martyn N. Paley, and Allan A. Pacey. "NMR spectroscopy of live human asthenozoospermic and normozoospermic sperm metabolism." Reproduction and Fertility 3, no. 2 (2022): 77–89. http://dx.doi.org/10.1530/raf-21-0101.

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Sperm motility varies between ejaculates from different men and from individual men. We studied normozoospermic and asthenozoospermic ejaculates after density-gradient centrifugation washing (DCG, 80/40%) and compared high- (80%) and low (40%)-motility sperm populations within the same sample. Our objective was to identify differences in endogenous metabolomes and energy metabolism in relation to sperm motility. 1H-Nuclear Magnetic Resonance spectroscopy (NMR) measured the endogenous metabolome of live human sperm. Incubating sperm with 13C-labelled substrates detected energy metabolism by 13C

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8

Hosseinchi, Mohammadreza, and Gholamreza Najafi. "Protective Effects of Hydroalcoholic Extract of Achillea Millefolium on In-vitro Fertilization in Streptozotocin-Induced Diabetic Male Mice." Qom Univ Med Sci J 16, no. 11 (2023): 928–41. http://dx.doi.org/10.32598/qums.16.11.2747.1.

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Background and Objectives: Diabetes is one of the determining factors in fertility that causes disturbances in spermatogenesis, ovulation and embryo formation. Achillea Millefolium (AM), as a medicinal plant with strong antioxidant and anti-inflammatory properties, is used to treat many diseases. This study aims to evaluate the protective effect of AM hydroalcoholic extract on sperm parameters and In-Vitro Fertilization (IVF) rate in mice. Methods: In this study, 40 mice were divided into five groups; control group, diabetic group, diabetic+low AM (receiving 37.5 mg/kg of AM extract daily), di

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Habeeb, A. O., H. M. H. Habeeb, and O. A. Mohammed. "Effect of Vitamin K Additive to Tris-Egg Yolk Extender on Iraqi Buffalo Bull Semen Characteristics." IOP Conference Series: Earth and Environmental Science 1449, no. 1 (2025): 012001. https://doi.org/10.1088/1755-1315/1449/1/012001.

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Abstract Buffalo sperm are sensitive to peroxidation of lipids because buffalo spermatozoa contain highly polyunsaturated free fatty acid (PUFA) which reduces the bull fertility following cryopreservation compared to other species. The current study was carried out to determine the effect of vitamin K supplementation in tris extender on Iraqi buffalo semen following treatment, cooling, and cryopreservation. Four Iraqi buffalo bulls were used in the current study. Samples were collected from all bulls accumulated and extended in tris extender with vitamin K at 0 (control), 0.03, 0.06, 0.09 ml/1

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Veznik, Z., D. Svecova, A. Zajicova, and P. Prinosilova. "Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity." Veterinární Medicína 48, No. 8 (2012): 221–28. http://dx.doi.org/10.17221/5773-vetmed.

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38 ejaculates of dogs of various breeds from2to 6 years old were examined in a short-term 120 min survival test. The examination focused on the functional parameters of sperm (motility, percentage of live sperma&shy;tozoa, acrosomal integrity) and their morphology. The levels of relationship between individual criteria of sperm analysis were demonstrated. Statistically highly significant correlations between motility, ratio of live spermatozoa, percentage of intact acrosomes and percentage of spermatozoa with altered superficial structures were proven. Our findings proved the sensitivity o

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Groos, Sabine, Walter Krause, and Ulrich O. Mueller. "Men with subnormal sperm counts live shorter lives." Biodemography and Social Biology 53, no. 1-2 (2006): 46–60. http://dx.doi.org/10.1080/19485565.2006.9989116.

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12

Marchesi, D. E., D. Petrilli, E. Hawkins, W. Xuimei, H. L. Feng, and A. Hershlag. "How long does sperm live?" Fertility and Sterility 96, no. 3 (2011): S78—S79. http://dx.doi.org/10.1016/j.fertnstert.2011.07.304.

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13

Iskandar, Sofjan, Rufika Mardalestari, Resmi Hernawati, Enok Mardiah, and Endang Wahyu. "The effect of kinds and concentration of cryoprotectant and thawing methods on frozen semen of Arab chicken." Jurnal Ilmu Ternak dan Veteriner 11, no. 1 (2012): 34–38. https://doi.org/10.14334/jitv.v11i1.505.

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The success of freezing chicken semen is the hope for preserving Indonesian native chickens. Semen from twenty Arab roosters were collected using massage technique once in a week. Cryoprotectant DMA (dimethyl acetamide) or DMF (dimethyl formalmide) of 7 or 9% and thawing A at temperature of 30oC for 30 seconds or in B at 5oC for 5 minutes. The volume of fresh semen was 0.3 Â± 0.072 ml/ejaculate, white colour, rather thick to thick, with 2200 Â± 372 millions sperms/ml and pH 6.95 Â± 0.32, 4+/3+ mass movement, 80% motility, 84 Â± 4.48% and abnormality of 14.75 Â± 1.28%. There were not statistica

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14

Sokolowska, A., B. Macías García, L. González Fernández, C. Ortega-Ferrusola, J. A. Tapia, and F. J. Peña. "Activated caspases are present in frozen–thawed canine sperm and may be related to post thaw sperm quality." Zygote 17, no. 4 (2009): 297–305. http://dx.doi.org/10.1017/s0967199409005401.

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SummaryThe identification of early changes in the sperm plasmalemma is currently a factor in the improvement of freezing protocols. We analysed the presence of active caspases in freeze–thawed (FT) dog spermatozoa, and evaluated straws from eight dogs using flow cytometry and fluorescence microscopy with fluorescein isothyocyanate–Val–Ala–Asp–fluoromethylketone (FITC–VAD–fmk) combined with ethidum homodimers. Apoptotic-like changes were evaluated using the YO–PRO-1/ethidium homodimer combination, and changes in mitochondrial membrane potential were monitored with JC-1. Sperm motility post-thaw

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15

HAZARIKA, D., S. SARMA, P. CHAKRAVARTY, D. J. KALITA, J. GOSWAMI, and B. C. DEKA. "Testosterone in yak bull seminal plasma and its correlation with physical characteristics of semen." Indian Journal of Animal Sciences 82, no. 10 (2012): 1193–94. http://dx.doi.org/10.56093/ijans.v82i10.24304.

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Studies were conducted to estimate testosterone concentration in seminal plasma and its correlation with some seminal characteristics of yak semen. The colour of semen varied from milky white to creamy. The average mean values for ejaculate volume, initial sperm motility, sperm concentration, pH, live sperm and testosterone were 2.83±0.08 ml, 71.5±0.57 %, 1182.5±3.23 million/ ml, 6.34±0.02, 80.05±0.74 % and 1.82±0.08 ng/ml respectively. Significant positive correlation existed between testosterone concentration in seminal plasma with initial sperm motility and live sperm count. The present stu

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16

Fatematuzzohora, Mst, Md Azizul Haque, Md Rafikul Islam, Md Azharul Hoque, and Md Younus Ali. "Evaluation of reproductive performance of indigenous cows using Brahman cross-bred bull in a participatory breeding program." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 2 (2016): 173–81. http://dx.doi.org/10.3329/aajbb.v1i2.61556.

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The present study was conducted at the Artificial Insemination Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University and 3 selected regions of Mymensingh district (Bhabakhali, Boera and Douhakhula) to find out the reproductive performance of indigenous cows using Brahman cross-bred bull in a participatory breeding program. Semen characteristics of Brahman cross-bred breeding bulls were ejaculate volume, mass motility, sperm concentration and live sperm percentage were evaluated. A total of 1087 indigenous cows and four Brahman cross-bred breeding bulls

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17

Paz, R. C. R., T. O. Morgado, C. T. R. Viana, F. P. Arruda, D. O. Bezerra do Nascimento, and L. D’A Guimarães. "Semen collection and evaluation of captive coatis (Nasua nasua)." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 64, no. 2 (2012): 318–22. http://dx.doi.org/10.1590/s0102-09352012000200010.

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Semen samples (n=105) were collected through eletroejaculation from six adult male coatis (Nasua nasua) between January 2007 and December 2008 at Universidade Federal de Mato Grosso Zoo, Cuiabá, Brazil. Mean values were: volume (mL); concentration (sperm/mL); total motility (%); progressive sperm motility (scale, 0-5); live spermatozoa (%); acrossome integrity (%); primary defects (%); and secondary defects (%). There was high correlation between total motility and live sperm; total motility and progressive sperm motility; total motility and acrossome integrity; live sperm and progressive moti

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18

Nosrati, Reza, Max M. Gong, Maria C. San Gabriel, Claudio E. Pedraza, Armand Zini, and David Sinton. "Paper-Based Quantification of Male Fertility Potential." Clinical Chemistry 62, no. 3 (2016): 458–65. http://dx.doi.org/10.1373/clinchem.2015.250282.

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Abstract BACKGROUND More than 70 million couples worldwide are affected by infertility, with male-factor infertility accounting for about half of the cases. Semen analysis is critical for determining male fertility potential, but conventional testing is costly and complex. Here, we demonstrate a paper-based microfluidic approach to quantify male fertility potential, simultaneously measuring 3 critical semen parameters in 10 min: live and motile sperm concentrations and sperm motility. METHODS The device measures the colorimetric change of yellow tetrazolium dye to purple formazan by the diapho

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Hussien Mohammad Tayawi and Sahib Jumaa Abdurrahman. "Comparative study of the carnitine, Gensing, Dill, and Arginine effect in sperm of normal and experimentally H2O2 induced oxidative stres albino male rats." Tikrit Journal of Pure Science 22, no. 8 (2023): 48–55. http://dx.doi.org/10.25130/tjps.v22i8.851.

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The aim of this study is to compare the effects of Carnitine, Gensing, Dill and Arginine in characteristics of the sperms:total sperm, percentage of live and dead sperm, normal and abnormal sperm in male normal and exposuedrats to oxidative stress induced by hydrogen peroxide H2O2(0.5%) in drinking water during the experiment for 30 days. Fifty male rats used in age 12-14 weeks in weights (300-325g) divided randomly into 10 groups, included 5 in each group as following.  Control, H2O2 , Carnitine , carnitine + H2O2 , Gensing , Gensing + H2O2, Dill, Dill+ H2O2, Arginine, Arginine + H2O2 g

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20

Beran, J., L. Stádník, J. Bezdíček, F. Louda, J. Čítek, and J. Ducháček. "Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing." Archives Animal Breeding 55, no. 3 (2012): 207–18. http://dx.doi.org/10.5194/aab-55-207-2012.

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Abstract. The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analyse

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Burnell, Mark. "The use of sexed semen in dairy herds." Livestock 24, no. 6 (2019): 282–86. http://dx.doi.org/10.12968/live.2019.24.6.282.

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Artificial insemination of cattle became a commercial reality in 1936, and in 1952 the first calves were born as a result of freeze-thawed semen insemination. The long-held aspiration for pre determination of sex became a reality with the development of sperm sorting technology with the first female calves being born in the UK in 1999. While most breeding companies supply sexed semen sorted by the original flow cytometry method, Genus ABS have developed the method of sperm deactivation. Whatever the method sexing accuracy exceeds 90% and conception rates can be expected to be comparable to con

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Mignini Renzini, M., M. Dal Canto, M. C. Guglielmo, et al. "Sperm donation: an alternative to improve post-ICSI live birth rates in advanced maternal age patients." Human Reproduction 36, no. 8 (2021): 2148–56. http://dx.doi.org/10.1093/humrep/deab148.

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Abstract STUDY QUESTION Can sperm donation increase live birth rates following ICSI in advanced maternal age (AMA) patients? SUMMARY ANSWER Sperm donation increases the live birth rate in AMA ICSI cycles. WHAT IS KNOWN ALREADY In ICSI practice, sperm donation has been predominantly applied to overcome male infertility. The involvement of paternal age and lower sperm quality in the severe reduction in fertility observed in AMA patients remains to be clarified. STUDY DESIGN, SIZE, DURATION Retrospective multicenter cohort study including data generated between 2015 and 2019 from 755 ICSI cycles

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Sultana, F., SS Husain, A. Khatun, AS Apu, and MAMY Khandoker. "Study on buck evaluation based on semen quality and fertility." Bangladesh Journal of Animal Science 42, no. 2 (2013): 101–8. http://dx.doi.org/10.3329/bjas.v42i2.18487.

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The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B1, B2, B3, B4 and B5) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility,

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Nolan, Kathleen, Cappy Miles Rothman, and Judith Wilson Ross. "Case Studies: Live Sperm, Dead Bodies." Hastings Center Report 20, no. 1 (1990): 33. http://dx.doi.org/10.2307/3562972.

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Gnezdilova, Larisa A., Pavel N. Abramov, and Seidfatima M. Borunova. "Synthetic media for diluting sperm products of agricultural producers and the biological role of individual components." Veterinariya, Zootekhniya i Biotekhnologiya 4, no. 113 (2023): 166–76. http://dx.doi.org/10.36871/vet.zoo.bio.202304018.

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Dilution of the ejaculate of male producers is one of the ways to prolong the life of sperm. Diluent is a synthetic (artificial) medium that provides long–term preservation of morphological parameters before and after cryopreservation and high fertilizing ability of sperm. Synthetic media are more saturated with nutritional and auxiliary components than sperm plasma (biological medium), since sperm live in plasma for only a few hours, and with a diluent for up to several days. Artificial media and their components used for dilution and storage of sperm should be investigated for harmlessness a

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Jeong, Mina, Seul Ki Kim, Hoon Kim, Jung Ryeol Lee, Byung Chul Jee, and Seok Hyun Kim. "Predictive value of sperm motility before and after preparation for the pregnancy outcomes of intrauterine insemination." Clinical and Experimental Reproductive Medicine 48, no. 3 (2021): 255–61. http://dx.doi.org/10.5653/cerm.2021.04469.

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Objective: This study aimed to investigate sperm motility and its changes after preparation as predictors of pregnancy in intrauterine insemination (IUI) cycles.Methods: In total, 297 IUI cycles from January 2012 to December 2017 at a single tertiary hospital were retrospectively analyzed. Patient and cycle characteristics, and sperm motility characteristics before and after processing were compared according to clinical pregnancy or live birth as outcomes.Results: The overall clinical pregnancy rate per cycle was 14.5% (43/297) and the live birth rate was 10.4% (30/289). Patient and cycle cha

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Almekaty, Khaled Mohamed, Mohamed Hasan Zahran, Mohamed Lotfy Amer, et al. "Factors predicting the outcome of percutaneous epididymal sperm aspiration in men with obstructive azoospermia." Archivio Italiano di Urologia e Andrologia 94, no. 3 (2022): 323–27. http://dx.doi.org/10.4081/aiua.2022.3.323.

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Objectives: To report on the live birth rates (LBRs) following percutaneous epididymal sperm aspiration (PESA) in men with obstructive azoospermia (OA) and factors affecting treatment outcome which is under reported in the literature.Methods: This is a multicenter study that was conducted in Egypt including all couples undergoing intra cytoplasmic sperm injection (ICSI) for OA using PESA-derived sperms. Men were subdivided according to aetiology into congenital, iatrogenic and idiopathic groups. Fertilization, pregnancy and LBRs were determined and compared in each group. The longitudinal LBR,

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Choi, Y. H., D. D. Varner, C. C. Love, D. L. Hartman, and K. Hinrichs. "Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse." REPRODUCTION 142, no. 4 (2011): 529–38. http://dx.doi.org/10.1530/rep-11-0145.

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Work with lyophilized sperm helps delineate the factors required for successful fertilization. We investigated the use of lyophilized sperm in equine embryo production. In Experiment 1, sperm DNA fragmentation index was not affected by three freeze/thaw or lyophilization cycles. In Experiment 2, oocytes injected with lyophilized sperm or with sperm from a treatment in which lyophilized sperm were suspended in sperm cytoplasmic extract (SE) yielded blastocyst development rates of 0 and 28% respectively (P<0.05). In Experiment 3, blastocyst development rate was 6–11% after injection of sperm

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Bisiau, Christian, Paula Moffett, James Graham, and Patrick McCue. "Comparison of Nanoparticles and Single-Layer Centrifugation for Separation of Dead from Live Stallion Spermatozoa." Veterinary Sciences 11, no. 7 (2024): 307. http://dx.doi.org/10.3390/vetsci11070307.

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The goal of this study was to compare the efficacy of coated iron-core nanoparticles and single-layer centrifugation for separation of dead from live stallion spermatozoa. Our hypothesis was that nanoparticles would bind to dead sperm and allow for separation from live sperm using a magnet, resulting in a population of spermatozoa with a high percentage of total and progressive motility. Treatment Group 1 was an untreated control. Treatment Group 2 (nanoparticles, NP) utilized sperm incubated with nanoparticles followed by application of a magnet to remove dead sperm adhered to the coated nano

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Gamidov, S. I., R. I. Ovchinnikov, A. Yu Popova, V. V. Polozov, N. P. Naumov, and N. G. Gasanov. "EFFECTIVENESS OF ASSISTED REPRODUCTIVE TREATMENT PROGRAMS DEPENDING ON THE CHARACTERISTICS OF SPERMOGRAM CHANGES." Andrology and Genital Surgery 19, no. 2 (2018): 82–87. http://dx.doi.org/10.17650/2070-9781-2018-19-2-82-87.

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Introduction. There’re some pathological mechanisms of male fertility disorders that still don’t have proper diagnostic tests. This significantly decreases diagnostic value of a spermogram and makes the problem of evaluation of the characteristics of spermogram changes and their effects on the effectiveness of assisted reproductive technologies (ART) a pressing problem.The study objectiveis to identify the correlation between effectiveness of ART programs and the characteristics of spermogram changes, in particular sperm concentration, motility, and morphology Materials and methods. At the V.I

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Simmons, Leigh W., Amy Denholm, Chantelle Jackson, Esther Levy, and Ewa Madon. "Male crickets adjust ejaculate quality with both risk and intensity of sperm competition." Biology Letters 3, no. 5 (2007): 520–22. http://dx.doi.org/10.1098/rsbl.2007.0328.

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Sperm competition theory predicts that males should increase their expenditure on the ejaculate with increasing risk of sperm competition, but decrease their expenditure with increasing intensity. There is accumulating evidence for sperm competition theory, based on examinations of testes size and/or the numbers of sperm ejaculated. However, recent studies suggest that ejaculate quality can also be subject to selection by sperm competition. We used experimental manipulations of the risk and intensity of sperm competition in the cricket, Teleogryllus oceanicus . We found that males produced eja

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Ezea, J., J. C. Ezike, D. C. Ifenkwe, H. O. Uzegbu, and M. A. Oguike. "Semen Characteristics and Specific Sperm Changes in Pubertal Boars Fed with Aidan (<i>Tetrapleura tetraptera</i>) Pod Meal." Nigerian Journal of Biotechnology 41, no. 1 (2024): 66–72. http://dx.doi.org/10.4314/njb.v41i1.7.

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A total of eighteen crossbred (Large White X Duroc) weaner boars, aged 6 weeks, averaging 9.47kg, were used to study the impact of Aidan pod meal (APM) in a 16-week experimental period. The boars were divided into three treatment groups and assigned 0.0% (control), 2.5% or 5.0% APM per unit weight of feed. The experiment was randomized. Data were collected on semen quality and specific changes in morphometry and morphology of sperm. Mass motility, live and normal sperm proportions were reduced (P<0.05) at 5.0% APM. Sperm concentration and total viable sperm were reduced (P<0.05) at both

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You, Jae Bem, Yihe Wang, Christopher McCallum, et al. "Live sperm trap microarray for high throughput imaging and analysis." Lab on a Chip 19, no. 5 (2019): 815–24. http://dx.doi.org/10.1039/c8lc01204k.

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Gonzalez-Castro, R. A., J. K. Graham, and E. M. Carnevale. "176 Localization and Quantitative Expression of Phospholipase C Zeta in Equine Sperm Using Commercial Antibodies." Reproduction, Fertility and Development 30, no. 1 (2018): 228. http://dx.doi.org/10.1071/rdv30n1ab176.

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Fertilization failure in vivo and in vitro (intracytoplasmic sperm injection, ICSI) can be caused by the inability of sperm to elicit intracellular calcium oscillations and to induce oocyte activation. Phospholipase C zeta (PLCz) is sperm-associated protein that can induce oocyte activation. Male infertility has been associated with PLCz deficiency in various species, although this has not been studied in the stallion. We hypothesised that the location and amount of PLCz on sperm varies among stallions. The aim of this study was to validate commercial antibodies (Ab) to detect PLCz on stallion

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MOL, Ben W., Lan N. VUONG, Tam M. LUU, et al. "Can We Identify Subgroups in Which ICSI is Cost-Effective Over IVF: A Secondary Analysis of a Randomised Clinical Trial." Fertility & Reproduction 04, no. 03n04 (2022): 152. http://dx.doi.org/10.1142/s2661318222740644.

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Background: The use of intracytoplasmic sperm injection (ICSI) has increased substantially worldwide, primarily in couples with non-male factor infertility. We recently published the results of a large randomised controlled trial (RCT) comparing ICSI and IVF showing no significant improvement of ICSI over IVF in couples with normal semen concentration and motility. Here, we assess the cost-effectiveness of ICSI in these couples, with additional attention for subgroups. Aim: To assess the effectiveness and cost-effectiveness of ICSI versus IVF in relation to sperm morphology. Method: We perform

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Solomon, I. P., E. F. Istifanus, and E. J. Idang. "Effect of frequency of ejaculation and graded dietary levels of Roselle (Hibiscussabdariffa) calyx meal supplementation on sperm profile of mongrel rabbit bucks." Nigerian Journal of Animal Production 49, no. 5 (2022): 162–73. http://dx.doi.org/10.51791/njap.v49i5.3780.

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Changes in semen quality occasioned by ejaculation frequency is one of the important yet neglected factor considered in artificial insemination. Several factors have been shown to influence semen parameters, one of which is sexual abstinence. Rabbits have a very short interval between successive ejaculations hence, semen can easily be collected for quality examination. This experiment was carried out to evaluate the effect of frequency of ejaculation and graded dietary levels of Roselle calyx meal supplementation on sperm profile of mongrel rabbit bucks. Twenty healthy mongrel rabbit bucks wer

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Usman, Tijjani Haruna, Saleh Mohammed Sir, and Ma’aruf Bashir Sani. "Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria." Malaysian Journal of Fundamental and Applied Sciences 15, no. 2-1 (2019): 303–6. http://dx.doi.org/10.11113/mjfas.v15n2-1.1540.

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The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal a

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Catalini, Laura, Jens Fedder, Bente Mertz Nørgård, and Line Riis Jølving. "Assisted Reproductive Technology Results Using Donor or Partner Sperm: A Danish Nationwide Register-Based Cohort Study." Journal of Clinical Medicine 12, no. 7 (2023): 2571. http://dx.doi.org/10.3390/jcm12072571.

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This was a nationwide cohort study based on Danish health registers focusing on assisted reproductive technology (ART) treatments in women using donor or partner sperm from 2007 to 2017. Women using donor sperm were subdivided into groups based on relationship status: women with male partners, single women, or women with female partners. The live birth adjusted odds ratios (aORs) after the IUI treatments in women using donor sperm compared with women using partner sperm were 1.48 (95% CI: 1.38–1.59) in women with male partners using donor sperm, 1.20 (95% CI: 1.13–1.28) in single women, and 1.

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Gabriel Sánchez-Partida, L., Gwendalyn Maginnis, Tanja Dominko, et al. "Live Rhesus Offspring by Artificial Insemination Using Fresh Sperm and Cryopreserved Sperm1." Biology of Reproduction 63, no. 4 (2000): 1092–97. http://dx.doi.org/10.1095/biolreprod63.4.1092.

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Parmar, K. H., FS Kavani, TK Patbandha, and SS Parikh. "Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 15, no. 03 (2020): 45–48. http://dx.doi.org/10.21887/ijvsbt.15.3.12.

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The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40

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Zhu, Yuqin, Nengyong Ouyang, Xiaohui Ji, Qingxue Zhang, and Haijing Zhao. "Clinical Pregnancy and Live Birth Outcomes after Intracytoplasmic Injection of Fresh versus Frozen Testicular Sperm." Andrologia 2024 (April 20, 2024): 1–5. http://dx.doi.org/10.1155/2024/3802703.

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This study aimed to investigate the potential benefits of frozen testicular sperm and to retrospectively analyze the clinical pregnancy and live birth outcomes following intracytoplasmic injection of fresh or frozen testicular sperm. A total of 468 infertile couples undergoing intracytoplasmic sperm injection cycles utilizing either fresh or frozen testicular sperm extracted between January 2017 and December 2021 were included in this analysis. Participants were categorized into two groups: those utilizing fresh testicular sperm (n = 324) and those utilizing frozen testicular sperm (n = 144).

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Shanmugam, M., U. Rajkumar, M. R. Reddy, and S. V. Rama Rao. "Effect of age on semen quality in naked neck and dwarf chicken under tropical climatic conditions." Animal Production Science 52, no. 10 (2012): 964. http://dx.doi.org/10.1071/an12033.

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The present study was undertaken to elucidate the effect of age on semen quality of naked neck and dwarf chicken lines under tropical climatic conditions. Semen collected by abdominal massage at 4-week intervals from 24 to 48 weeks of age was evaluated for different semen quality parameters. Except sperm concentration and appearance, all other parameters were significantly (P < 0.05) affected by the age of the birds. The semen volume, motility, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye reduction test and percentage of live sperm were significantly (P < 0.05) lower

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Karim, Md Fazlul, MAA Yahia Khandaker, and Syed Sakhawat Husain. "Comparative efficacy of two extenders on post-thaw sperm characteristics of cryopreserved Black Bengal buck semen." Research in Agriculture Livestock and Fisheries 6, no. 1 (2019): 119–25. http://dx.doi.org/10.3329/ralf.v6i1.41393.

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The research work was conducted at the Artificial Insemination (AI) Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh to compare the efficacy between Egg Yolk Citrate (EYC) and Tris extenders for cryopreserved semen production in Black Bengal buck. The parameters of semen characteristics included percentage of individual motility (progressive), normal and live sperm and sperm concentration (billion/ml). Statistical analysis of fresh semen of individual buck effect was found to be significant (p<0.01) on normal sperm percentage but in

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Zhong, Jingjing, Zhiyong Lu, Ning Ma, et al. "The Impact of Seasonal Variation on Clinical Pregnancy and Live Birth Rates in Assisted Reproductive Technology: A Retrospective Cohort Study in Hainan." Journal of Clinical and Nursing Research 8, no. 8 (2024): 243–50. http://dx.doi.org/10.26689/jcnr.v8i8.8191.

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Objective: To investigate the influence of season on live birth and clinical pregnancy rates, as well as assisted reproductive technology (ART) outcomes, in the Hainan region. Methods: Patients were categorized into four groups based on the dates of artificial insemination and transplantation: spring, summer, autumn, or winter. The main outcome measures were clinical pregnancy rates and live birth rates. Secondary outcomes included body mass index (BMI), oocyte number, two pronuclei (2PN) cleavage rate, total gonadotropin (Gn) dosage and days, age, 2PN fertilization rate, sperm concentration,

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Chérasse, Sarah, and Serge Aron. "Impact of immune activation on stored sperm viability in ant queens." Proceedings of the Royal Society B: Biological Sciences 285, no. 1893 (2018): 20182248. http://dx.doi.org/10.1098/rspb.2018.2248.

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Ant queens mate on a single occasion early in life and store millions of sperm cells in their spermatheca. By carefully using stored sperm to fertilize eggs, they can produce large colonies of thousands of individuals. Queens can live for decades and their lifetime reproductive success is dependent on their ability to keep stored sperm alive. Maintaining high sperm viability requires metabolic energy which could trade-off with other costly processes such as immunity. We tested the impact of immune activation on the survival of stored sperm by prompting Lasius niger ant queens to mount a melani

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khaleel, Abdulmuttaleb Hazim, and Salih Hassan Al-Azzawi. "Polymorphisms Detection in Heat Shock Brotein70 Gene (HSP70) and its Association with Semen Quality in Local Iraqi Goats." Diyala Agricultural Sciences Journal 14, no. 1 (2022): 21–30. http://dx.doi.org/10.52951/dasj.22140103.

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Heat shock protein 70 (HSP70) is a vital gene affecting semen quality traits. The study aimed to estimate the breeding values additive dominance variance of Iraqi black goat semen quality and investigate the effect of allele substitution in heat shock protein 70 gene (HSP70) carpine on some semen quality traits (volume, mass motility, a live sperm, and sperm concentration). DNA isolated from 15 Iraqi black goats was subjected to PCR amplification of the caprine HSP70 gene. Used Single-nucleotide polymorphism (SNP) to detect the variant DNA fragments that were sequenced. Synonymous Genotypes we

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Révay, T., A. Kovács, W. Rens, and I. Gustavsson. "Simultaneous detection of viability and sex of bovine spermatozoa." Reproduction, Fertility and Development 14, no. 6 (2002): 373. http://dx.doi.org/10.1071/rd02026.

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The viability and sex of bovine spermatozoa were simultaneously evaluated. After viability and acrosome staining with trypan blue/Giemsa, only live spermatozoa became decondensed by a modified papain-dithiothreitol method. Owing to this specific effect, live sperm heads were easily distinguished by their enlarged size and dark violet colour from small, light blue dead sperm heads. In the same sperm sample, X- and Y-chromosome-bearing sperm were distinguished by their fluorescent signal, using fluorescence in situ hybridization (FISH) with an XY paint set and 4,6-diamino-2-phenylindole counters

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Hassan, M. S., and S. M. Eidan. "EFFECT OF SWIM-UP AND GLASS WOOL TECHNIQUES, WITH ADDING ANTIOXIDANTS TO TRIS EXTENDER ON IMPROVING POST-CRYOPRESERVED SOME SEMEN ATTRIBUTES OF LOW SEMEN QUALITY FOR HOLSTEIN BULLS." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 52, no. 3 (2021): 552–63. http://dx.doi.org/10.36103/ijas.v52i3.1342.

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This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving some post-cryopreserved semen quality for Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and g

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Xavier, Sophia, Hiron M. Harshan, Jayakumar C, Leeba Chacko, Shynu M, and Elizabeth Kurian. "Semen Characteristics and Protein Contents in SeminalPlasma of Vechur Bulls." Indian Journal of Animal Reproduction 45, no. 1 (2024): 48–52. https://doi.org/10.48165/ijar.2024.45.01.11.

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The present investigation was carried out to study the semen characteristics of Vechur bulls. A total of 36 ejaculates collected from six Vechur bulls were studied. The overall mean ejaculate volume, colour, pH, mass activity, progressive sperm motility and total seminal plasma protein concentrations were analysed. The mean ejaculate volume noted was 2.26 ± 0.36 mL, colour of semen ranged from milky to thick milky, pH was 6.87 ± 0.02, density from DDD to DDDD, mass activity +++ to ++++ and sperm progressive motility was 78.1 ± 1.05 %. Significant differences in ejaculate volume, mass activity,

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Hatırnaz, Şafak, Serdar Başaranoğlu, Ebru Hatırnaz, and Mine Kanat Pektaş. "Fresh Versus Frozen Testicular Sperm Samples in Microdissection Testicular Sperm Extraction Intracytoplasmic Sperm Injection Treatment." Gynecology Obstetrics & Reproductive Medicine, February 20, 2017, 1. http://dx.doi.org/10.21613/gorm.2016.636.

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<p><strong>Objective:</strong> The present study aims to compare the clinical outcomes of fresh versus frozen testicular samples in patients with non-obstructive azoospermia who would undergo intracytoplasmic sperm injection procedure.<br /><strong>Study Design:</strong> This is a retrospective review of 541 patients with non-obstructive azoospermia who consecutively underwent microdissection testicular sperm injection and intracytoplasmic sperm injection between January 2010 and October 2014.<br /><strong>Results:</strong> A total of 4896

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