Academic literature on the topic 'LJ proportion method'

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Journal articles on the topic "LJ proportion method"

1

HUANG, Zi-Kun, Qing LUO, Bi-Xia JIANG, et al. "Performance of the microscopic observation drug susceptibility assay in pyrazinamide susceptibility testing for Mycobacterium tuberculosis." Chinese Medical Journal 126, no. 22 (2013): 4334–39. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20130108.

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Background Drug susceptibility assay is very important in tuberculosis therapy. Pyrazinamide is a first line antituberculosis drug and diagnosis of its resistance in Mycobacterium tuberculosis (M. tuberculosis) is difficult and time consuming by conventional methods. In this study, we aimed to evaluate the performance of the microscopic observation drug susceptibility (MODS) assay in the detection of pyrazinamide resistance in M. tuberculosis relative to the conventional Wayne assay and Lowenstein-Jensen (LJ) proportion method. Methods M. tuberculosis clinical isolates (n=132) were tested by t
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Zadbuke, Sonali Sudhir, Reena Set, Nishat Khan, and Jayanthi Shastri. "Concurrent evaluation of microscopic observation of drug susceptibility assay for pulmonary and extrapulmonary tuberculosis." Journal of Laboratory Physicians 9, no. 02 (2017): 089–94. http://dx.doi.org/10.4103/0974-2727.199626.

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Abstract BACKGROUND: Methods for detection and drug susceptibility of tuberculosis (TB) with solid media are inexpensive but slow and laborious. Rapid methods to diagnose TB and multidrug-resistant TB (MDR-TB) are a global priority for TB control. OBJECTIVES: A study was performed to compare the sensitivity of detection of mycobacterial growth and time of culture positivity by microscopic observation of drug susceptibility (MODS) assay with that of Lowenstein–Jensen (LJ) culture in pulmonary and extrapulmonary TB and to evaluate the concordance of the susceptibilities to isoniazid (INH) and ri
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Wahyuningrum, Retno, Ritmaleni Ritmaleni, Tatang Irianti, Subagus Wahyuono, and Takushi Kaneko. "ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD." Asian Journal of Pharmaceutical and Clinical Research 11, no. 3 (2018): 132. http://dx.doi.org/10.22159/ajpcr.2018.v11i3.22587.

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Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowenstein–Jensen (LJ) medium.Result: The result of this study showed that ethanolic extract and fra
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Lamsal, A., B. Thapa, M. Timilshina, SC Verma, GR Ghimire, and A. Gautam. "Direct Nitrate Reductase Assay for detection of drug resistance in Mycobacterium tuberculosis: rapid, simple and inexpensive method for low resource laboratories." International Journal of Infection and Microbiology 2, no. 2 (2013): 34–38. http://dx.doi.org/10.3126/ijim.v2i2.8319.

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INTRODUCTION: The most common method for detection of drug-resistant-Tuberculosis (DR-TB) in resource-limited settings (RLSs) is indirect susceptibility testing on Lowenstein-Jensen (LJ) medium with results available only after 2-3 months. Rapid detection of drug resistance by direct Nitatre Reductase Assay (NRA) expedites Tuberculosis patient management. The objective of the study is to access the feasibility and performance of Direct NRA for detection of DR-TB in National Tuberculosis Center under the National Tuberculosis Control Programme (NTP). MATERIALS AND METHODS: Out of 416 previously
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Yusuf, Roomi, Tehmeena Wani, Gulnaz Bashir, and Nargis Bali. "Drug Susceptibility Testing of M. tuberculosis to Isoniazid and Rifampicin- A Comparison between MTT Tube Method and LJ Proportion Method." British Journal of Medicine and Medical Research 9, no. 2 (2015): 1–6. http://dx.doi.org/10.9734/bjmmr/2015/18688.

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de Almeida, Isabela Neves, Suely Conceição Alves da Silva, Haliton Alves de Oliveira Junior, et al. "The Activity-Based Cost of Drug-Susceptibility Test of Mycobacterium tuberculosis Through Kit SIRE Nitratase® Plastlabor." International Journal of Mycobacteriology 9, no. 1 (2020): 24–28. http://dx.doi.org/10.4103/ijmy.ijmy_8_20.

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Background: Drug-resistant tuberculosis (TB) is an ongoing health threat, and the greatest challenge to adequate control of TB in many countries lies in the lack of proper laboratory drug-susceptibility test. The aim of this study was to evaluate the activity-based costs (ABC) of Kit SIRE Nitratase® (Kit SIRE) and compare its values with the conventional drug-susceptibility test. Methods: The ABC was calculated for three different approaches: Kit SIRE (clinical samples and cultures), proportion methods in Lowenstein Jensen (PM-LJ), and the Bactec™ MGIT™ 960 system based on Mycobacterial Resear
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Rahman, S. M. Mazidur, Md Fahim Ather, Rumana Nasrin, et al. "Performance of WHO-Endorsed Rapid Tests for Detection of Susceptibility to First-Line Drugs in Patients with Pulmonary Tuberculosis in Bangladesh." Diagnostics 12, no. 2 (2022): 410. http://dx.doi.org/10.3390/diagnostics12020410.

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The fast and accurate detection of susceptibility in drugs is a major challenge for a successful tuberculosis (TB) control programme. This study evaluated the performance of WHO-endorsed rapid diagnostic tools, such as BACTEC MGIT 960 SIRE (MGIT SIRE), GenoType MTBDRplus (MTBDRplus) and Xpert MTB/RIF (Xpert), for detecting susceptibility to first-line anti-TB drugs among pulmonary TB patients in Bangladesh. A total of 825 sputum samples with results from drug susceptibility testing (DST) against first-line anti-TB drugs in the MGIT SIRE, MTBDRplus and Xpert assays were evaluated and compared w
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8

K., Mamuda, S. Olonitola O., D. Jatau E., and Nicholas E. "Evaluation of Nitrate Reductase Assay for Detection of Multi-drug Resistant Mycobacterium tuberculosis among Patients at National Tuberculosis Reference Laboratory Zaria Nigeria." Journal of Advances in Medical and Pharmaceutical Sciences 14, no. 1 (2017): 1–7. https://doi.org/10.9734/JAMPS/2017/33705.

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<strong>Aims: </strong>To evaluate nitrate reductase assay for detection of multi-drug resistant <em>Mycobacterium tuberculosis</em> among patients at National Tuberculosis Reference Laboratory Zaria Nigeria. <strong>Study Design:</strong> Hospital based cross sectional study <strong>Place and Duration of Study:</strong> National Tuberculosis Reference Laboratory Zaria Nigeria from December 2015 to June 2016. <strong>Methodology:</strong> A total of 437 re-treatment patients’ samples were screened for Acid Fast Bacilli (AFB), 72 were smear positive. Out of 72 smears positive, 62 were culture p
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Zodape, G. V., S. N. Dharmashale, and V. S. Gaikwad. "Effect of Piper nigrum (Linn.) seeds extract and second line anti-tuberculosis drugs on a few Mycobacterium tuberculosis strains." Journal of Applied and Natural Science 13, no. 1 (2021): 402–6. http://dx.doi.org/10.31018/jans.v13i1.2497.

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Piper nigrum (Linn.) belonging to the family Piperaceae have been reported for its multitudinous medicinal values. The present study was undertaken to examine the direct effect of Ethionamide (ETH), Para amino salicylic acid (PAS), ethanolic extracts of P. nigrum on Mycobacterium tuberculosis (MTB) strain H37Rv and Multi drug-resistant (MDR)-strains-12, 19 and 21. The proportion method was used to detect the anti-mycobacterial drug susceptibility testing for mycobacteria using Lowenstein Jensen (LJ) medium. It was found that P. nigrum does not interfere with single or in the combination of bot
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10

Hu, Peilei, Liqiong Bai, Fengping Liu, et al. "Evaluation of the Xpert MTB/RIF assay for diagnosis of tuberculosis and rifampin resistance in county-level laboratories in Hunan province, China." Chinese Medical Journal 127, no. 21 (2014): 3744–50. http://dx.doi.org/10.3760/cma.j.issn.0366-6999.20141004.

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Background The Xpert MTB/RIF showed high sensitivity and specificity in previous studies carried out in different epidemiological and geographical settings and patient populations in high-burden tuberculosis (TB) countries. However, there were little data obtained by validation or demonstration study of the assay in China. In this study, the performance of Xpert MTB/RIF was investigated in two county-level laboratories in Hunan Province, China. Methods Consecutive patients with suspected pulmonary tuberculosis (PTB) and suspicion for multidrug-resistant tuberculosis (MDR-TB) were enrolled. For
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