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Journal articles on the topic 'Metabolic oligosaccharide engineering'

Author: Grafiati

Published: 10 September 2021

Last updated: 30 July 2025

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1

Gilormini, Pierre-André, Anna R. Batt, Matthew R. Pratt, and Christophe Biot. "Asking more from metabolic oligosaccharide engineering." Chemical Science 9, no. 39 (2018): 7585–95. http://dx.doi.org/10.1039/c8sc02241k.

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Metabolic Oligosaccharide Engineering (MOE) is a groundbreaking strategy which has been largely used in the last decades, as a powerful strategy for glycans understanding. The present review aims to highlight recent studies that are pushing the boundaries of MOE applications.

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2

Dube, D. "Metabolic oligosaccharide engineering as a tool for glycobiology." Current Opinion in Chemical Biology 7, no. 5 (2003): 616–25. http://dx.doi.org/10.1016/j.cbpa.2003.08.006.

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3

Campbell, Christopher T., Srinivasa-Gopalan Sampathkumar, and Kevin J. Yarema. "Metabolic oligosaccharide engineering: perspectives, applications, and future directions." Molecular BioSystems 3, no. 3 (2007): 187. http://dx.doi.org/10.1039/b614939c.

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4

Whitman, Chad M., Fan Yang, and Jennifer J. Kohler. "Modified GM3 gangliosides produced by metabolic oligosaccharide engineering." Bioorganic & Medicinal Chemistry Letters 21, no. 17 (2011): 5006–10. http://dx.doi.org/10.1016/j.bmcl.2011.04.128.

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5

Zheng, Xiu-Jing, Fan Yang, Mingwei Zheng, Chang-Xin Huo, Ye Zhang, and Xin-Shan Ye. "Improvement of the immune efficacy of carbohydrate vaccines by chemical modification on the GM3 antigen." Organic & Biomolecular Chemistry 13, no. 22 (2015): 6399–406. http://dx.doi.org/10.1039/c5ob00405e.

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6

Möller, Heinz, Verena Böhrsch, Joachim Bentrop, Judith Bender, Stephan Hinderlich, and Christian P. R. Hackenberger. "Glycan-Specific Metabolic Oligosaccharide Engineering of C7-Substituted Sialic Acids." Angewandte Chemie International Edition 51, no. 24 (2012): 5986–90. http://dx.doi.org/10.1002/anie.201108809.

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7

Almaraz, Ruben T., Mohit P. Mathew, Elaine Tan, and Kevin J. Yarema. "Metabolic Oligosaccharide Engineering: Implications for Selectin-Mediated Adhesion and Leukocyte Extravasation." Annals of Biomedical Engineering 40, no. 4 (2011): 806–15. http://dx.doi.org/10.1007/s10439-011-0450-y.

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Späte, Anne-Katrin, Verena F. Schart, Sophie Schöllkopf, Andrea Niederwieser, and Valentin Wittmann. "Terminal Alkenes as Versatile Chemical Reporter Groups for Metabolic Oligosaccharide Engineering." Chemistry - A European Journal 20, no. 50 (2014): 16502–8. http://dx.doi.org/10.1002/chem.201404716.

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9

Späte, Anne-Katrin, Verena F. Schart, Sophie Schöllkopf, Andrea Niederwieser, and Valentin Wittmann. "Terminal Alkenes as Versatile Chemical Reporter Groups for Metabolic Oligosaccharide Engineering." Chemistry - A European Journal 20, no. 50 (2014): 16411. http://dx.doi.org/10.1002/chem.201405618.

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10

Ruffing, Anne, Zichao Mao, and Rachel Ruizhen Chen. "Metabolic engineering of Agrobacterium sp. for UDP-galactose regeneration and oligosaccharide synthesis." Metabolic Engineering 8, no. 5 (2006): 465–73. http://dx.doi.org/10.1016/j.ymben.2006.05.004.

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11

Almaraz, Ruben T., Udayanath Aich, Hargun S. Khanna, et al. "Metabolic oligosaccharide engineering with N-Acyl functionalized ManNAc analogs: Cytotoxicity, metabolic flux, and glycan-display considerations." Biotechnology and Bioengineering 109, no. 4 (2011): 992–1006. http://dx.doi.org/10.1002/bit.24363.

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12

Heise, Torben, Christian Büll, Daniëlle M. Beurskens, et al. "Metabolic Oligosaccharide Engineering with Alkyne Sialic Acids Confers Neuraminidase Resistance and Inhibits Influenza Reproduction." Bioconjugate Chemistry 28, no. 7 (2017): 1811–15. http://dx.doi.org/10.1021/acs.bioconjchem.7b00224.

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13

Sminia, Tjerk J., Han Zuilhof, and Tom Wennekes. "Getting a grip on glycans: A current overview of the metabolic oligosaccharide engineering toolbox." Carbohydrate Research 435 (November 2016): 121–41. http://dx.doi.org/10.1016/j.carres.2016.09.007.

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14

Tan, Elaine, Ruben T. Almaraz, Hargun S. Khanna, Jian Du, and Kevin J. Yarema. "Experimental Design Considerations for In Vitro Non-Natural Glycan Display via Metabolic Oligosaccharide Engineering." Current Protocols in Chemical Biology 2, no. 3 (2010): 171–94. http://dx.doi.org/10.1002/9780470559277.ch100059.

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15

Piñero, Tamara, Valnice J. Peres, Alejandro Katzin, and Alicia S. Couto. "Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry." Molecular and Biochemical Parasitology 182, no. 1-2 (2012): 88–92. http://dx.doi.org/10.1016/j.molbiopara.2011.12.008.

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16

Dharmarha, Vrinda, Christopher Saeui, Jian Song, Hui Li, Howard Katz, and Kevin Yarema. "AB107. P081. Metabolic oligosaccharide engineering of pancreatic cells: measurement of sialylation and identification of sialylated glycoproteins." Annals of Pancreatic Cancer 1, no. 1 (2018): AB107. http://dx.doi.org/10.21037/apc.2018.ab107.

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17

Kitowski, Annabel, and Gonçalo J. L. Bernardes. "A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection." ChemBioChem 21, no. 18 (2020): 2696–700. http://dx.doi.org/10.1002/cbic.202000226.

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18

Du, Jian, Pao-Lin Che, Zhi-Yun Wang, Udayanath Aich, and Kevin J. Yarema. "Designing a binding interface for control of cancer cell adhesion via 3D topography and metabolic oligosaccharide engineering." Biomaterials 32, no. 23 (2011): 5427–37. http://dx.doi.org/10.1016/j.biomaterials.2011.04.005.

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19

Nan, Lijing, Jiao Li, Wanjun Jin, et al. "Comprehensive quali-quantitative profiling of neutral and sialylated O-glycome by mass spectrometry based on oligosaccharide metabolic engineering and isotopic labeling." RSC Advances 9, no. 28 (2019): 15694–702. http://dx.doi.org/10.1039/c9ra01114e.

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20

Möller, Heinz, Verena Böhrsch, Lothar Lucka, Christian P. R. Hackenberger, and Stephan Hinderlich. "Efficient metabolic oligosaccharide engineering of glycoproteins by UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) knock-down." Molecular BioSystems 7, no. 7 (2011): 2245. http://dx.doi.org/10.1039/c1mb05059a.

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21

Wang, Yan, Nan Zhang, Shanshan Lu, et al. "Dual-Monitoring Glycosylation and Local pH in Live Cells by Metabolic Oligosaccharide Engineering with a Ratiometric Fluorescent Tag." Analytical Chemistry 91, no. 21 (2019): 13720–28. http://dx.doi.org/10.1021/acs.analchem.9b03047.

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22

Möller, Heinz, Verena Böhrsch, Christian P. R. Hackenberger, and Stephan Hinderlich. "N-Azidoacetylmannosamine and N-Azidoacetylgalactosamine Incorporation into N-Glycans of Recombinantly Expressed Human Lactotransferrin by Metabolic Oligosaccharide Engineering." Journal of Carbohydrate Chemistry 30, no. 4-6 (2011): 334–46. http://dx.doi.org/10.1080/07328303.2011.608140.

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23

Möller, Heinz, Verena Böhrsch, Joachim Bentrop, Judith Bender, Stephan Hinderlich, and Christian P. R. Hackenberger. "Inside Cover: Glycan-Specific Metabolic Oligosaccharide Engineering of C7-Substituted Sialic Acids (Angew. Chem. Int. Ed. 24/2012)." Angewandte Chemie International Edition 51, no. 24 (2012): 5766. http://dx.doi.org/10.1002/anie.201203242.

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24

Späte, Anne-Katrin, Verena F. Schart, Sophie Schöllkopf, Andrea Niederwieser, and Valentin Wittmann. "Cover Picture: Terminal Alkenes as Versatile Chemical Reporter Groups for Metabolic Oligosaccharide Engineering (Chem. Eur. J. 50/2014)." Chemistry - A European Journal 20, no. 50 (2014): 16401. http://dx.doi.org/10.1002/chem.201490206.

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25

Späte, Anne-Katrin, Verena F. Schart, Julia Häfner, Andrea Niederwieser, Thomas U. Mayer, and Valentin Wittmann. "Expanding the scope of cyclopropene reporters for the detection of metabolically engineered glycoproteins by Diels–Alder reactions." Beilstein Journal of Organic Chemistry 10 (September 22, 2014): 2235–42. http://dx.doi.org/10.3762/bjoc.10.232.

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Monitoring glycoconjugates has been tremendously facilitated by the development of metabolic oligosaccharide engineering. Recently, the inverse-electron-demand Diels–Alder reaction between methylcyclopropene tags and tetrazines has become a popular ligation reaction due to the small size and high reactivity of cyclopropene tags. Attaching the cyclopropene tag to mannosamine via a carbamate linkage has made the reaction even more efficient. Here, we expand the application of cyclopropene tags to N-acylgalactosamine and N-acylglucosamine derivatives enabling the visualization of mucin-type O-gly

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26

Qin, Yang, Hee-Jong Woo, Kong-Sik Shin, Myung-Ho Lim, and Seong-Kon Lee. "Comparative transcriptome profiling of different tissues from beta-carotene-enhanced transgenic soybean and its non-transgenic counterpart." Plant Cell, Tissue and Organ Culture (PCTOC) 140, no. 2 (2019): 341–56. http://dx.doi.org/10.1007/s11240-019-01731-2.

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Abstract Beta-carotene-enhanced transgenic soybeans, harboring genes encoding phytoene synthase and carotene desaturase under the control of a seed-specific promoter, were developed to alleviate vitamin A deficiency in populations, the diet of which was deficient in this vitamin. However, metabolic engineering of carotenoid biosynthetic pathways often has unintended effects, leading to major metabolic changes in plants that harbor endogenous beta-carotene biosynthesis pathways. In the present study, we performed transcriptome profiling analysis using RNA-seq to investigate the changes in the t

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27

Xiong, De-Cai, Jingjing Zhu, Ming-Jie Han, et al. "Rapid probing of sialylated glycoproteins in vitro and in vivo via metabolic oligosaccharide engineering of a minimal cyclopropene reporter." Organic & Biomolecular Chemistry 13, no. 13 (2015): 3911–17. http://dx.doi.org/10.1039/c5ob00069f.

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28

Huxley, Kathryn E., and Lianne I. Willems. "Chemical reporters to study mammalian O-glycosylation." Biochemical Society Transactions 49, no. 2 (2021): 903–13. http://dx.doi.org/10.1042/bst20200839.

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Glycans play essential roles in a range of cellular processes and have been shown to contribute to various pathologies. The diversity and dynamic nature of glycan structures and the complexities of glycan biosynthetic pathways make it challenging to study the roles of specific glycans in normal cellular function and disease. Chemical reporters have emerged as powerful tools to characterise glycan structures and monitor dynamic changes in glycan levels in a native context. A variety of tags can be introduced onto specific monosaccharides via the chemical modification of endogenous glycan struct

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29

van Scherpenzeel, Monique, Federica Conte, Christian Büll, et al. "Dynamic tracing of sugar metabolism reveals the mechanisms of action of synthetic sugar analogs." Glycobiology 32, no. 3 (2021): 239–50. http://dx.doi.org/10.1093/glycob/cwab106.

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Abstract Synthetic sugar analogs are widely applied in metabolic oligosaccharide engineering (MOE) and as novel drugs to interfere with glycoconjugate biosynthesis. However, mechanistic insights on their exact cellular metabolism over time are mostly lacking. We combined ion-pair ultrahigh performance liquid chromatography–triple quadrupole mass spectrometry mass spectrometry using tributyl- and triethylamine buffers for sensitive analysis of sugar metabolites in cells and organisms and identified low abundant nucleotide sugars, such as UDP-arabinose in human cell lines and CMP-sialic acid (CM

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30

Kambhampati, Shrikaar, Jose A. Aznar-Moreno, Sally R. Bailey, et al. "Temporal changes in metabolism late in seed development affect biomass composition." Plant Physiology 186, no. 2 (2021): 874–90. http://dx.doi.org/10.1093/plphys/kiab116.

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Abstract The negative association between protein and oil production in soybean (Glycine max) seed is well-documented. However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes. In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accum

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31

Abd El Latif, Maha. "EFFECT OF COMMERCIAL ENZYMES AND\OR MANNAN OLIGOSACCHARIDE SUPLEMENTATION ON PRODUCTIVE PERFORMANCE, NUTRITIVE VALUES AND METABOLIC INDICES OF BROILER CHICKS." Egyptian Journal of Animal Production 60, no. 2 (2023): 61–75. http://dx.doi.org/10.21608/ejap.2023.189621.1057.

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32

Alanzi, Abdullah R., Ananiya A. Demessie, and Taifo Mahmud. "Biosynthesis and metabolic engineering of pseudo-oligosaccharides." Emerging Topics in Life Sciences 2, no. 3 (2018): 405–17. http://dx.doi.org/10.1042/etls20180010.

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Pseudo-oligosaccharides are microbial-derived secondary metabolites whose chemical structures contain pseudosugars (glycomimetics). Owing to their high resemblance to the molecules of life (carbohydrates), most pseudo-oligosaccharides show significant biological activities. Some of them have been used as drugs to treat human and plant diseases. Because of their significant economic value, efforts have been put into understanding their biosynthesis, optimizing their fermentation conditions, and engineering their metabolic pathways to obtain better production yields. Many unusual enzymes partici

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33

Martinez-Anaya, M. A., and O. Rouzaud. "Influence of wheat flour and Lactobacillus strains on the dynamics of by-products from amylolytic activities / Influencia de la harina de trigo y de la especie de Lactobacilo en la dinámicade subproductos de la actividad amilolítica." Food Science and Technology International 3, no. 2 (1997): 123–36. http://dx.doi.org/10.1177/108201329700300207.

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Amylolytic activity of six flours from three European wheat cultivars (Obelisk, Camp Remy and Fresco, at 70 and 100% extraction level), and five lactobacilli strains (Lactobacillus plantaracm B-39, L-73; L. brevis 25a, L-62, and L. sanfrancisco L-99), as well as the dynamics of by-products from amylolytic degrading action in mixed flour-lactobacilli systems have been investigated. α-Amylase activity of flours depended on ash content; whole flours from the three cultivars had similar values, which were of the order of three to ten times that of white flours. L. sanfrancisco showed the lowest α-

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34

Ling, Meixi, Jianghua Li, Guocheng Du, and Long Liu. "Metabolic engineering for the production of chitooligosaccharides: advances and perspectives." Emerging Topics in Life Sciences 2, no. 3 (2018): 377–88. http://dx.doi.org/10.1042/etls20180009.

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Chitin oligosaccharides (CTOs) and its related compounds chitosan oligosaccharides (CSOs), collectively known as chitooligosaccharides (COs), exhibit numerous biological activities in applications in the nutraceutical, cosmetics, agriculture, and pharmaceutical industries. COs are currently produced by acid hydrolysis of chitin or chitosan, or enzymatic techniques with uncontrollable polymerization. Microbial fermentation by recombinant Escherichia coli, as an alternative method for the production of COs, shows new potential because it can produce a well-defined COs mixture and is an environme

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35

Jones, Meredith B., Noboru Tomiya, Michael J. Betenbaugh, and Sharon S. Krag. "Analysis and metabolic engineering of lipid-linked oligosaccharides in glycosylation-deficient CHO cells." Biochemical and Biophysical Research Communications 395, no. 1 (2010): 36–41. http://dx.doi.org/10.1016/j.bbrc.2010.03.117.

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36

Zhu, Yingying, Hongzhi Cao, Hao Wang, and Wanmeng Mu. "Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges." Current Opinion in Biotechnology 78 (December 2022): 102841. http://dx.doi.org/10.1016/j.copbio.2022.102841.

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37

Palur, Dileep Sai Kumar, Shannon R. Pressley, and Shota Atsumi. "Microbial Production of Human Milk Oligosaccharides." Molecules 28, no. 3 (2023): 1491. http://dx.doi.org/10.3390/molecules28031491.

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Human milk oligosaccharides (HMOs) are complex nonnutritive sugars present in human milk. These sugars possess prebiotic, immunomodulatory, and antagonistic properties towards pathogens and therefore are important for the health and well-being of newborn babies. Lower prevalence of breastfeeding around the globe, rising popularity of nutraceuticals, and low availability of HMOs have inspired efforts to develop economically feasible and efficient industrial-scale production platforms for HMOs. Recent progress in synthetic biology and metabolic engineering tools has enabled microbial systems to

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38

Kwak, Suryang, Scott J. Robinson, Jae Won Lee, Hayoon Lim, Catherine L. Wallace, and Yong-Su Jin. "Dissection and enhancement of prebiotic properties of yeast cell wall oligosaccharides through metabolic engineering." Biomaterials 282 (March 2022): 121379. http://dx.doi.org/10.1016/j.biomaterials.2022.121379.

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39

Li, Jian, Honghao Li, Huayi Liu, and Yunzi Luo. "Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast." Journal of Fungi 9, no. 9 (2023): 907. http://dx.doi.org/10.3390/jof9090907.

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Natural sugar substitutes are safe, stable, and nearly calorie-free. Thus, they are gradually replacing the traditional high-calorie and artificial sweeteners in the food industry. Currently, the majority of natural sugar substitutes are extracted from plants, which often requires high levels of energy and causes environmental pollution. Recently, biosynthesis via engineered microbial cell factories has emerged as a green alternative for producing natural sugar substitutes. In this review, recent advances in the biosynthesis of natural sugar substitutes in yeasts are summarized. The metabolic

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40

Yi, Zhen, Xiao Luo, and Lei Zhao. "Research Advances in Chitosan Oligosaccharides: From Multiple Biological Activities to Clinical Applications." Current Medicinal Chemistry 27, no. 30 (2020): 5037–55. http://dx.doi.org/10.2174/0929867326666190712180147.

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Chitosan oligosaccharides (COS), hydrolysed products of chitosan, are low-molecular weight polymers with a positive charge and good biocompatibility. COS have recently been reported to possess various biological activities, including hypoglycaemic, hypolipidaemic, antioxidantantioxidant, immune regulation, anti-inflammatory, antitumour, antibacterial, and tissue engineering activities, exhibiting extensive application prospects. Currently, the biological processes and mechanisms of COS are attractive topics of study, ranging from the genetic, molecular and protein levels. This article reviews

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41

Thomas, Reeba, Tamo Fukamizo, and Wipa Suginta. "Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities." Molecules 28, no. 18 (2023): 6591. http://dx.doi.org/10.3390/molecules28186591.

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Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN. These sugars are of practical importance because of their various biological activities, such as antimicrobial, anti-inflammatory, antioxidant and antitumor activities, as well as triggering the innate immunity in plants. The reported data on bioactivities of COSs used to contain some uncertainties or contradictions, because the experiments were conducted with poorly characterized COS mixtures. Recently,

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42

Loncar, Eva, Radomir Malbasa, and Ljiljana Kolarov. "Kombucha fermentation on raw extracts of different cultivars of Jerusalem artichoke." Acta Periodica Technologica, no. 38 (2007): 37–44. http://dx.doi.org/10.2298/apt0738037l.

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Kombucha is a symbiosis between yeasts and acetic bacteria. It usually grows on sweetened black tea, but cultivation is possible on many other substrates. Jerusalem artichoke tubers extract is one of them. Tubers are suitable for the dietetic nutrition because of the low monosaccharide content and presence of some polyfructan ingredients which act as prebiotic. Five different cultivars of Jerusalem artichoke were used for the preparation of substrates for kombucha fermentation. The aim of this paper was the investigation of the influence of different Jerusalem artichoke cultivars on metabolic

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43

Roy, Anindita, Yuma Miyai, Alessandro Rossi, et al. "Metabolic engineering of non-pathogenic Escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific heparosan oligosaccharides." Biochimica et Biophysica Acta (BBA) - General Subjects 1865, no. 1 (2021): 129765. http://dx.doi.org/10.1016/j.bbagen.2020.129765.

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44

Villéger, Romain, Emilie Pinault, Karine Vuillier-Devillers, et al. "Prebiotic Isomaltooligosaccharide Provides an Advantageous Fitness to the Probiotic Bacillus subtilis CU1." Applied Sciences 12, no. 13 (2022): 6404. http://dx.doi.org/10.3390/app12136404.

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Bacillus subtilis CU1 is a probiotic strain with beneficial effects on immune health in elderly subjects and diarrhea. Commercialized under spore form, new strategies to improve the germination, fitness and beneficial effects of the probiotic once in the gut have to be explored. For this purpose, functional food ingredients, such as isomaltooligosaccharides (IMOSs), could improve the fitness of Bacillus probiotics. IMOSs are composed of α(1→6)- and α(1→4)-linked oligosaccharides and are partially indigestible. Dietary IMOSs stimulate beneficial members of intestinal microbiota, but the effect

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45

Johnson, Athira, Fanbin Kong, Song Miao, Sabu Thomas, Sabah Ansar, and Zwe-Ling Kong. "In-Vitro Antibacterial and Anti-Inflammatory Effects of Surfactin-Loaded Nanoparticles for Periodontitis Treatment." Nanomaterials 11, no. 2 (2021): 356. http://dx.doi.org/10.3390/nano11020356.

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Periodontitis is an inflammatory disease associated with biofilm formation and gingival recession. The practice of nanotechnology in the clinical field is increased overtime due to its potential advantages in drug delivery applications. Nanoparticles can deliver drugs into the targeted area with high efficiency and cause less damages to the tissues. In this study, we investigated the antibacterial and anti-inflammatory properties of surfactin-loaded κ-carrageenan oligosaccharides linked cellulose nanofibers (CO-CNF) nanoparticles. Three types of surfactin-loaded nanoparticles were prepared bas

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46

Park, Bu-Soo, Jihee Yoon, Jun-Min Lee, et al. "Metabolic engineering of Priestia megaterium for 2’-fucosyllactose production." Microbial Cell Factories 24, no. 1 (2025). https://doi.org/10.1186/s12934-024-02620-w.

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Abstract Background 2′-Fucosyllactose (2′-FL) is a predominant human milk oligosaccharide that significantly enhances infant nutrition and immune health. This study addresses the need for a safe and economical production of 2’-FL by employing Generally Recognized As Safe (GRAS) microbial strain, Priestia megaterium ATCC 14581. This strain was chosen for its robust growth and established safety profile and attributing suitable for industrial-scale production. Results The engineering targets included the deletion of the lacZ gene to prevent lactose metabolism interference, introduction of α-1,2-

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47

Tian, Xiao, Lingna Zheng, Changjiang Wang, et al. "Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection." Nature Communications 14, no. 1 (2023). http://dx.doi.org/10.1038/s41467-023-44118-w.

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AbstractMetabolic oligosaccharide engineering (MOE) is a classical chemical approach to perturb, profile and perceive glycans in physiological systems, but probes upon bioorthogonal reaction require accessibility and the background signal readout makes it challenging to achieve glycan quantification. Here we develop SeMOE, a selenium-based metabolic oligosaccharide engineering strategy that concisely combines elemental analysis and MOE,enabling the mass spectrometric imaging of glycome. We also demonstrate that the new-to-nature SeMOE probes allow for detection, quantitative measurement and vi

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48

Gray, Taylor, Kristin Labasan, Gour Daskhan, et al. "Synthesis of 4-azido sialic acid for testing against Siglec-7 and in metabolic oligosaccharide engineering." RSC Chemical Biology, 2025. https://doi.org/10.1039/d5cb00030k.

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An important approach for tracking and visualizing sialic acid-containing glycans involves using sialic acid reporters functionalized with bioorthogonal handles. More specifically, metabolic oligosaccharide engineering (MOE) commonly employs monosaccharides with an...

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49

Rigolot, Vincent, Yannick Rossez, Christophe Biot, and Cedric Lion. "A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule in Escherichia coli." RSC Chemical Biology, 2023. http://dx.doi.org/10.1039/d2cb00219a.

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Most Escherichia coli strains associated with neonatal meningitis express the K1 capsule, a sialic acid polysaccharide that is directly related to their pathogenicity. Metabolic oligosaccharide engineering (MOE) has mostly been...

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50

Cioce, Anna, Ganka Bineva-Todd, Anthony J. Agbay, et al. "Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase." ACS Chemical Biology, April 9, 2021. http://dx.doi.org/10.1021/acschembio.1c00034.

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