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Journal articles on the topic 'Methanol leaf extract'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Sapkota, Sujana, Alishma Maharjan, Sanjeeta Tiwari, and Meena Rajbhandari. "Phytochemical Analysis, Antioxidant Potential and Antibacterial Activities of Different Anatomical Parts of Hypericum cordifolium Choisy." Scientific World Journal 2024 (May 25, 2024): 1–11. http://dx.doi.org/10.1155/2024/8128813.

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The genus Hypericum comprises a large number of species. The flower, leaf, stem, and root of the Hypericum species are widely used in traditional medicine in different cultures. Many Hypericum species have been well investigated phytochemically and pharmacologically. However, only a few reports are available on the H. cordifolium native to Nepal. The present study aims to evaluate the phytochemical composition of different extracts, qualitative analysis of methanol extract of the flower and leaf using thin-layer chromatography (TLC), and the antioxidant properties of components by the TLC-DPPH

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Lenny, Sovia, Murni Suryani Lumbantoruan, and Helmina Br Sembiring. "Antioxidant Activity Assay of Eucalyptus pellita Leaf Extract." International Journal of Ecophysiology 5, no. 2 (2023): 26–33. http://dx.doi.org/10.32734/ijoep.v5i2.13715.

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In pharmacy, eucalyptus leaves are used for various pharmacological activities because they contain phenolic compounds. This study aims to detect phytochemical compounds and test the antioxidant activity of Eucalyptus pellita leaf extract. The dry powder of E. pellita leaves (1900 g) was macerated first with methanol solvent. The concentrated methanol extract was then subjected to partition extraction with n - hexane solvent. The methanol layer was concentrated and then partitioned with ethyl acetate solvent. Methanol extract was obtained as much as 176.75 g (9,30%), n-hexane extract 13.77 g (

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Suresh, B., S. Shabana, M. Satya Prasad, M. R. Darukamallia, and A. Krishna Satya. "Phytochemical Studies and GC-MS Analysis of Tinospora Formanii Leaf Extracts." International Journal for Research in Applied Science and Engineering Technology 11, no. 9 (2023): 237–49. http://dx.doi.org/10.22214/ijraset.2023.55641.

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Abstract: The present study deals with the phytochemical aspects of Tinospora formanii. Crude leaf extracts were prepared with different solvents such as hexane, chloroform, methanol, and water using a Soxhlet apparatus. Qualitative and quantitative phytochemical studies and GC-MS analysis of leaf extracts have been carried out. Phytochemical studies showed that methanol leaf extract showed the presence of alkaloids, flavonoids, phenols, sterols, phytosterols, terpenoids, Tannins, saponins, glycosides, carbohydrates Anthraquinones, coumarins, Amino acids. Quantitative estimation showed that th

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Sati, S. C., and Savita Joshi. "Antibacterial Activities ofGinkgo bilobaL. Leaf Extracts." Scientific World JOURNAL 11 (2011): 2237–42. http://dx.doi.org/10.1100/2011/545421.

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The antibacterial activity of methanol, ethanol, chloroform, and hexane extracts of the leaves of Himalayan gymnospermous plantGinkgo bilobaL. was assessed against five animal and plant pathogenic strains (Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli) employing disc-diffusion and broth-dilution assays. The methanol extract showed the highest activity (zone of inhibition of 15–21 mm) followed by ethanol (14–19 mm), chloroform (15–20 mm), and hexane (14–19 mm) extracts at 250 μg/mL. A minimum inhibitory concentration (MIC) of 7.8

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Giri, Dil Prakash, and Meena Rajbhandari. "PHYTOCHEMICAL ANALYSIS AND CONSTITUENTS OF HEXANE EXTRACT OF MELASTOMA MALABATHRICUM L." Journal of Institute of Science and Technology 23, no. 1 (2018): 18–25. http://dx.doi.org/10.3126/jist.v23i1.22150.

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Hexane, dichloromethane, ethyl acetate, methanol and 50 % aqueous methanol extracts of leaf, flower and fruit of Melastoma malabathricum were screened for the presence of different classes of phytochemicals. In phytochemical screening, ethyl acetate, methanol and 50 % aqueous methanol extracts showed the presence of phenolics and flavonoids. Glycosides were found only in the 50 % methanol extract of fruit. Free radical scavenging activity of methanol extract of leaf, flower and fruit was determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical. Total phenolic content was measured spe

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Hernández-Fernández, Adrián, Yolanda Garrido, Eduardo Iniesta-López, Antonia Pérez de los Ríos, Joaquín Quesada-Medina, and Francisco José Hernández-Fernández. "Recovering Polyphenols in Aqueous Solutions from Olive Mill Wastewater and Olive Leaf for Biological Applications." Processes 11, no. 9 (2023): 2668. http://dx.doi.org/10.3390/pr11092668.

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Olive leaf and olive mill wastewater are wastes which are produced in olive industry and can be used to obtain value-added products such as polyphenols. In this work, extracts of polyphenols from olive mill wastewater and olive leaf in an aqueous medium are obtained and their composition in polyphenols were determined and compared with those obtained using the conventional extract agent (methanol–water). The antioxidant capacity of the aqueous extracts of olive mill wastewater and olive leaf were also analysed. It was observed that the olive mill wastewater extracted in water provides a simila

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Fabowale, P. O., O. Agunloye, and C. I. Adekannbi. "Comparative Screening of Phytochemicals and Bioactive Compounds of Trema orientalis (Linn. Blume) Leaf and Bark Extracts." Asian Journal of Research in Biochemistry 13, no. 2 (2023): 7–16. http://dx.doi.org/10.9734/ajrb/2023/v13i2251.

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This study compared the phytochemical constituents of the leaf with the Bark extracts of T. orientalis, using the same extraction solvents. The leaf and Bark of T. orientalis were harvested at Federal University of Technology, Akure forest, dried and pulverized into powder. Extract were prepared from the powdered plants using Methanol and N-hexane. The qualitative and quantitative phytochemical presents in the extracts were determined. The functional compounds of the leaf extract were determined by Fourier Transmission Infrared Spectrometry (FT-IR). Percentage yield of Methanol was better than

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R. Nair, Lekshmy, and M. Balasubrahmanian. "Correlation Between Phytochemicals and Antioxidant Activities of Different Leaf Extracts of Entada rheedii." Journal of Plant Science Research 39, no. 1 (2023): 199–208. http://dx.doi.org/10.32381/jpsr.2023.39.01.20.

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The main aim of this study was to determine Total Phenolic Content, Total Flavonoid Content, terpenoid content, steroid content and analyze the antioxidant activity of different leaf extracts of Entada rheedii. Correlation between antioxidant activities and total phenolic content, total flavonoids content, terpenoid content and steroid content were also analyzed. The total phenolic content in E. rheedii hexane, ethyl acetate, methanol, and aqueous leaf extracts were found to be 10.16 mg GAE/g, 24.73 mg GAE/g, 26.11 mg GAE/g, and 24.85 mg GAE/g sample dry weight respectively. The Total flavonoi

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Gunyakti, Ayse, Meltem Asan-Ozusaglam, and Muhammad Mujtaba. "Assessment of Biological and Antioxidant Capacities of Myrtuscommunis L. Leaf and Fruit Extracts from Mediterranean Region of Turkey." Journal of Microbiology and Biotechnology Research 7, no. 3 (2017): 16. http://dx.doi.org/10.24896/jmbr.2017733.

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Recently in humans and fishes most of the disease causing pathogenic microorganisms developed resistance against certain antimicrobial agents. This situation is causing both health and economic problems. In this context present study was designed to evaluate the antimicrobial and antioxidant activities of leaves and fruits of Myrtuscommunis. Antimicrobial activity of the plant’s leaf and fruit extracts was tested by using disc diffusion method against five fish, seven food-borne human pathogens and twoyeast strains. For this purpose, n-hexane and methanol an organic solvent were used for the o

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Patel, Geetha, Harshita M., Sathi D., and Padmaa M. Paarakh. "ANTICANCER STUDIES ON THE LEAVES OF TINOSPORA CORDIFOLIA (WILD) MIERS." International Journal of Advanced Research 10, no. 10 (2022): 1029–35. http://dx.doi.org/10.21474/ijar01/15578.

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The aim of the study was to evaluate anticancer activity of the methanol and aqueous extracts of leaves of Tinosporacordifolia Wild Miers against a Human Breast Carcinoma Cell line(MDAMB231) by MTT assay. The coarse powder of leaf were subjected to Soxhletextraction and microwave assisted extraction using methanol and water as solvent. The IC50 values of aqueous extract of leaf followed by soxhlation and methanol extract of leaf by microwave extraction were found to be 59.85Î¼g/mL and 249Î¼g/mLrespectively in MDAMB231 cells in comparison to Standard Vincristine 14.41ÂµM whereas methanol extrac

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Xạ, Trương Văn, Trần Kim Thoa, Nguyễn Đức Độ, and Nguyễn Đắc Khoa. "Seed Soaking Using Methanol Kalanchoe pinnata Leaf Extracts Induces Rice Resistance against Bacterial Leaf Blight." International Journal of Plant Biology 14, no. 4 (2023): 1155–66. http://dx.doi.org/10.3390/ijpb14040084.

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Bacterial leaf blight (Xanthomonas oryzae pv. oryzae) is a significant rice disease. Aqueous crude extracts of Kalanchoe pinnata were shown to induce rice resistance against the disease. This study aims at testing the disease-reducing effects of K. pinnata leaf extracts using the liquid–liquid extraction method with three different solvents (dichloromethane, methanol, and water). This serves as a basis to select appropriate extracts for effective disease control. Three concentrations (1, 1.5, and 2%) of each extract were tested using seed soaking. The extracts did not show adverse effects on s

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Ushie, O. A., B. D. Longbap, T. I. Azuaga, S. I. Iyen, D. I. Ugwuja, and R. F. Ijoko. "Phytochemical screening and proximate analysis of the leaf extracts of Vitex doniana." Scientia Africana 21, no. 1 (2022): 149–58. http://dx.doi.org/10.4314/sa.v21i1.13.

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The method of cold maceration was used in the extraction by serial exhaustive extraction method which involves successive extraction with solvents of increasing polarity from a nonpolar (hexane) to a more polar solvent (methanol) to ensure that a wide polarity range of compound could be extracted. The phytochemical screening of the crude extract of Vitex doniana showed that flavonoids, steroids, phlobatannins, alkaloids, saponins were found to be present. Alkaloids were detected in the methanol, n-Hexane, acetone and ethyl acetate extracts when Wagner`s reagent was used. Flavonoids were detect

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Bussaman, Prapassorn, Piyarat Namsena, Paweena Rattanasena, and Angsuman Chandrapatya. "Effect of Crude Leaf Extracts onColletotrichum gloeosporioides(Penz.) Sacc." Psyche: A Journal of Entomology 2012 (2012): 1–6. http://dx.doi.org/10.1155/2012/309046.

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Colletotrichum gloeosporioides(Penz.) Sacc. is a fungus that causes anthracnose disease in tropical fruit plants, resulting in damages of the fruit plants and low yield and quality of fruits. The use of chemical fungicides is common for management of this disease, but it also results in the development of fungal resistance to the chemicals. Therefore, this study aims toin vitroevaluate the efficacy of 14 crude leaf extracts againstC. gloeosporioides. The results showed thatPiper sarmentosumleaf extracts, using 80% of ethanol, methanol, and chloroform as solvents, were found to have very high a

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Adamu, U. O., A. Mann, J. O. Tijani, and Y. A. Kabir. "Evaluation of <i>in vitro</i> anti-trypanosomal activities of leaves, stem bark and root bark extracts of <i>Acacia nilotica</i> (L) Willd ex Del., <i>Guiera senegalensis</i> j. F. Gmel and <i>Ziziphus abyssinica</i> Hochst ex A. rich." Science World Journal 19, no. 1 (2024): 269–74. http://dx.doi.org/10.4314/swj.v19i1.37.

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Currently, the control and treatment of African trypanosomiasis are limited by the number of chemotherapeutic drugs with associated side effects. Consequently, there is an urgent need for a non-toxic herbal treatment for African trypanosomiasis. Leaf, stem, and root bark extracts of Acacia nilotica L. and Guiera senegalensis J. F. Gmel and Ziziphus abyssinica Hochst ex A. Rich were sequentially extracted using hexane, ethyl acetate, methanol and water as solvents and evaluated for in vitro anti-trypanosomal activities against Trypanosoma brucei, and phytochemical contents. Results revealed tha

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PERBONI, LAÍS TESSARI, DIRCEU AGOSTINETTO, CANDIDA RENATA JACOBSEN DE FARIAS, FÁBIO CLASEN CHAVES, and JÉSSICA RODRIGUES GARCIA. "ANTIFUNGAL ACTIVITY OF Heteranthera reniformis EXTRACTS AGAINST Bipolaris oryzae1." Revista Caatinga 34, no. 2 (2021): 339–49. http://dx.doi.org/10.1590/1983-21252021v34n210rc.

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ABSTRACT Plants synthesize specialized metabolites to defend themselves against pathogens. These active compounds, when isolated and identified, can be used as template for fungicide development. Based on field observations, Heteranthera reniformis (kidney leaf mudplantain) could potentially synthesize compounds with antifungal activity. The goal of this study was to evaluate the fungicidal activity of H. reniformis leaf extracts on Bipolaris oryzae development. The activities of hexane, ethyl acetate, and methanol extracts of H. reniformis leaves were tested on mycelial growth, sporulation, a

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Adeniyi, K. A., E. O. Ibeh, K. A. Oyibo-Usman, et al. "Cercaricidal efficacy of plant extract: evidence from the methanolic leaves and bark extracts of <i>Anacardium ocidentale</i> (Linnaeus)." Dutse Journal of Pure and Applied Sciences 9, no. 4b (2024): 60–70. http://dx.doi.org/10.4314/dujopas.v9i4b.7.

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Schistosomiasis continues to be a public health problem causing in unsatisfactorily high level of morbidity. Praziquantel is a recent medicine for treatment but unable to kill emerging schistosomes. It does not prevent re-infection and its constant application may lead to the advent of drug-resistant parasites. Qualitative phytochemical screening of the methanol crude extract of A. occidentale revealed the presence of Tannins, Flavonoids and Saponins. Cercaricidal activity on Gymnocephalous cercaria, after 120 minutes of contact in a concentration of 0.10 mg/mL, showed that there was 92 and 34

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Ningsih, Dian Riana, Zusfahair Zusfahair, and Dwi Kartika. "Identification of Secondary Metabolites Compounds and Antibacterial Activities on The Extract of Soursop Leaf." Molekul 11, no. 1 (2016): 101. http://dx.doi.org/10.20884/1.jm.2016.11.1.199.

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Treatment of bacterial infectious diseases using semi-synthetic antibiotics can lead to resistance, so as to overcome it necessary to search for natural ingredients from plant extracts that has potential as an antibacterial, one of which is the leaf extract of soursop (Annona muricata L.). This study aims to determine the antibacterial activity of soursop leaf against E. coli and identify groups most active chemical compounds from the extracts. Soursop leaves extracted by maceration using n-hexane, chloroform and methanol. The extracts were tested for antibacterial activity using the diffusion

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T.I, Abhinya, and Sajani Jose. "Phytochemical Analysis and Repellent Activity of Annona Muricata and Syzygium Samarangense Leaves Against Sitophilus Oryzae." YMER Digital 21, no. 06 (2022): 123–31. http://dx.doi.org/10.37896/ymer21.06/14.

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The present study is conducted to control the stored pest grain - Sitophilus oryzae using plant leaves, such as Annona muricata and Syzygium samarangense. Extraction of selected leaves were done using organic solvents such as Methanol and petroleum ether. Qualitative phytochemical analysis of selected plant leaves revealed the presence of secondary metabolites such as Alkaloids, Flavonoids, Tannins, Saponins, Glycerides, Steroids, Terpenoids, Phenols, Quinone and Phlobotannine, which have repellent activities against Sitophilus oryzae. Repellency test were also done using Methanol and petroleu

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Titus, Stephen Dio, Nelson Christian a, Achilus Francis, et al. "Chemical Constituents of Borassus Aethiopum Methanol Leaf and Fruit Extract." International Journal of Research Publication and Reviews 5, no. 9 (2024): 2421–26. http://dx.doi.org/10.55248/gengpi.5.0924.2641.

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Oyeleke, Oyedotun, Mutiat Bamigboye, Boyede Olowookere, Kehinde Alabi, Samuel Oladele, and Johnson Oladele. "Comparative Studies on the Phytochemical Constituents and in vitro Antioxidant Activities of Methanol and Ethyl Acetate Leaf Extracts of Talium Trangulare." Journal of Basic and Applied Research in Biomedicine 7, no. 1 (2021): 18–23. http://dx.doi.org/10.51152/jbarbiomed.v7i1.209.

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Medicinal herbs have been known for their use as an alternative medicine in the management and treatment of different categories of diseases. The present study was designed to assess the phytochemical constituents present in methanol and ethylacetate leaves extract of Talium trangulare and its antioxidant potentials against free radicals comparatively. The functional groups of the phytochemicals were carried out using FTIR techniques while the phytochemical component of the extract was determined by standard methods. The comparative studies of antioxidant abilities of methanol and ethylacetate

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Sultana, Munsifa, Sajan Das, Mohammad Shahriar, Rumana Akhter, Isa Naina Mohamed, and Mohiuddin Ahmed Bhuiyan. "In vitro Comparative Studies of the Leaf Extracts of Four Different Types of Calamus Species." Dhaka University Journal of Pharmaceutical Sciences 21, no. 2 (2022): 173–81. http://dx.doi.org/10.3329/dujps.v21i2.63118.

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The leaf extracts of Calamus tenius, Calamus viminalis, Calamus guruba and Calamus erectus possessed various phytochemicals including alkaloids, carbohydrates, saponins, phenols, tannins, flavonoids, glycosides, fixed oils and steroids. The point of the present investigation was to compare the antioxidant profile of different leaf extracts of these species. In the present study, methanol, ethanol and chloroform extract of leaf of C. tenius, C. viminalis, C. guruba and C. erectus were explored for in-vitro thrombolytic activity, membrane stabilizing activity by using hypotonic solution-induced

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Sidhartha, Elizabeth, John Jackson Yang, Renni Sherlin Novi Dimara, Fri Rahmawati, and Siska Wati Dewi Purba. "Phytochemical Screening, Antioxidant and Antifungal Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)." European Journal of Advanced Chemistry Research 5, no. 1 (2024): 1–8. http://dx.doi.org/10.24018/ejchem.2024.5.1.151.

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Anredera cordifolia (Ten.) Steenis (Binahong) is a medicinal plant that has been used by the indigenous Indonesian community to treat various ailments including surgical wounds, stomach ulcers, and skin problems such as itching and sores. Candida albicans is the most important species responsible for infections in patients with immunological problems associated with invasive fungal diseases. The resistance of Candida species to antifungal drugs has made scientists pay more attention to traditional herbal medicine. Binahong contains secondary metabolites such as alkaloids, flavonoids, polypheno

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Arumougame, Chanemougame Tangavelou, Gayathiri Ekambaram, and Byrappa Viswanathan Madepalli. "Hepatoprotective activity of Chamaecrista nigricans in Experimental Rats." International Journal of Plant Science and Horticulture 3, no. 1 (2021): 20–27. https://doi.org/10.36811/ijpsh.2021.110028.

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An important ethnomedicinal plant Chamaecrista nigricans (Vahl) Greene, widely used for antipyretic, appetite, family planning, fevers, sore throat, wounds and various gastrointestinal disorders including diarrhoea and peptic ulcer. In the present study, acute toxicity and hepatoprotective activity of methanol extract alone were carried out in experimental animals approved by the Institutional Animal Ethics Committee. In acute toxicity studies, there was no mortality in animals when the extracts tested as per OECD guidelines. Regarding hepatoprotective activity, methanol leaf extract significa

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Solomon, Ermiyas, Abiyou Tilahun, Belete Tewabe, Abebe Tedla, and Minbale Gashu. "Antimicrobial activity and Antioxidant Property of Osyris abyssinica." BERHAN INTERNATIONAL RESEARCH JOURNAL OF SCIENCE AND HUMANITIES 8, no. 1 (2024): 49–61. https://doi.org/10.61593/dbu.birjsh.01.08.03.

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Osyris abyssinica (Syn Osyris quadripartite, Qeret in Amharic) belongs to Santalaceae family which is locally used to treat diarrhea, sexually transmitted diseases, anemia, cancer and wounds. The phytochemical constituents of the plant extracts were qualitatively assessed by the standard protocols. Methanol extract of root, stem bark and leaf of O. abyssinica contained terpenoid, flavonoid, tannin and phenolic compounds. Saponin was present in methanol extract of stem bark and root but not on the leaf. Alkaloid, tannin and phenol were also found in ethyl acetate extract of all plant parts. The

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Humbare, Ravikiran B., Joyita Sarkar, Anjali A. Kulkarni, et al. "Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of Rubia cordifolia L. Extracts Prepared with Improved Extraction Conditions." Antioxidants 11, no. 5 (2022): 1006. http://dx.doi.org/10.3390/antiox11051006.

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Rubia cordifolia L. (Rubiaceae) is an important plant in Indian and Chinese medical systems. Extracts prepared from the root, stem and leaf have been used traditionally for the management of various diseases. Some of the known effects are anti-inflammation, neuroprotection, anti-proliferation, immunomodulation and anti-tumor. A comparative account of the extracts derived from different organs that lead to the identification of the most suitable solvent is lacking. We explored the presence of phytochemicals, antioxidant activity and anti-proliferative properties of a variety of solvent-based ex

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Daniel, Imaobong E., Ekemini I. Akpan, and Edidiong C. Utam. "Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum." Nepal Journal of Biotechnology 8, no. 1 (2020): 17–28. http://dx.doi.org/10.3126/njb.v8i1.30206.

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Antioxidant and antimicrobial activities of different extracts (methanol and ethyl acetate) of leaf and stem of Bryophyllum pinnatum were studied. The screening for the secondary metabolites was carried out using the standard methods. The antioxidant capacities of the different extracts were assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and Ferric reducing antioxidant power (FRAP) while the antimicrobial activity of the extracts obtained were screened against Gram-positive, Gram-negative bacteria and fungi (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa Salmonel

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Akhter, Rupaly, and Md Wadud Sarker. "Antimicrobial activity in leaf extract of Neem in broiler." Research in Agriculture Livestock and Fisheries 6, no. 2 (2019): 337–43. http://dx.doi.org/10.3329/ralf.v6i2.43063.

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The main objective of the study was to evaluate antibacterial activity of Neem leaf extracts (methanolic) against E. coli and Salmonella using different test such as production performances, biochemical, hematological, bacteriological tests and zone of Inhibition (ZOI) method. Azadirachta indica (neem) leaf extract was used to test growth performance, biochemical , hematological parameters and antimicrobial activity against disease causing bacteria Escherichia coli and Salmonella. That’s result indicated, methanolic extract of neem increased body weight, feed conversion ratio, decreased mortal

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Mohamad Shariff, Nurul Fatin Syazleen, Thangam Singgampalam, Chean Hui Ng, and Chin Siang Kue. "Antioxidant activity and zebrafish teratogenicity of hydroalcoholic Moringa oleifera L. leaf extracts." British Food Journal 122, no. 10 (2020): 3129–37. http://dx.doi.org/10.1108/bfj-02-2020-0113.

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PurposeMoringa oleifera (MO) is a herbal remedy that is rich in essential amino acids and phytochemicals in the leaves, seeds and pods. It is becoming a famous food in various ethnic groups, and the consumption is increasing worldwide. However, there is no safety profile of the hydroalcoholic MO leaf extracts, or documented teratogenicity aspect. This study was sought to compare the antioxidant activity and the teratogenicity of hydroalcoholic MO leaf extracts in the zebrafish embryo.Design/methodology/approachMO leaves were extracted with aqueous alcohols (ethanol or methanol: water, 80:20 v/

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Garbi, Mohammed I., Elbadri E. Osman, Ahmed S. Kabbashi, et al. "Cytotoxicity of Vitex trifolia leaf extracts on MCF-7 and Vero cell lines." Journal of Scientific and Innovative Research 4, no. 2 (2015): 89–93. http://dx.doi.org/10.31254/jsir.2015.4208.

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Pharmacological and preventive properties of Vitex trifolia leaf extracts are well known, but the anticancer activity of methanol and petroleum ether extracts of Vitex trifolia leafs on human breast cancer cells have not been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these extracts against MCF-7 and Vero cell line. Cells were exposed to 125 to 500 μg/ml of the extracts of Vitex trifolia for 72 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) assays. The resul

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khasim, shaik mahammad. "Phytochemical profiling and GC-MS analysis of Leaf Extracts of Dendrobium anceps Sw. (Orchidaceae)." Journal of Medical pharmaceutical and allied sciences 12, no. 6 (2023): 6230–40. http://dx.doi.org/10.55522/jmpas.v12i6.5876.

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Orchids have been used in various traditional medicinal systems for their potential therapeutic properties. Dendrobium anceps Sw. is an important medicinal orchid used to treat various diseases. The primary objective of this study is to analyze the phytochemical profile and conduct GC-MS analysis on different solvent extracts derived from D. anceps. The shade-dried leaf powder was subjected to cold extraction using n-hexane, acetone, ethyl acetate, and methanol as solvents. Qualitative phytochemical screening was then performed on the resulting crude leaf extracts, following established standa

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Awotedu BF, Omolola TO, Akala AO, Ogunsiji AO, Owoeye EA, and Olaoti-Laaro SO. "Radical Scavenging activity and phytochemical screening of the leaf extract of Synsepalum dulcificum (Schumach. & Thonn.) Daniell." World Journal of Advanced Research and Reviews 10, no. 3 (2021): 069–76. http://dx.doi.org/10.30574/wjarr.2021.10.3.0241.

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Antioxidants are chemicals that possess bioactive constituents which usually allow them to fight against free radicals. Antioxidant and phytochemicals properties of the leaves of Synsepalum dulcificum were investigated using ethyl-acetate, ethanol and methanol extracts. 2, 2-diphenyl-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, ferric reducing antioxidant power (FRAP), total flavonoid and the total phenolic content was carried out. The phytochemical screening assay was done on the following: Alkaloids, saponin, flavonoids, tannin, anthraquinones, terpenoids, cardiac g

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AGUSTINA, EVA. "UJI AKTIVITAS SENYAWA ANTIOKSIDAN DARI EKSTRAK DAUN TIIN (Ficus Carica Linn) DENGAN PELARUT AIR, METANOL DAN CAMPURAN METANOL-AIR." KLOROFIL: Jurnal Ilmu Biologi dan Terapan 1, no. 1 (2017): 38. http://dx.doi.org/10.30821/kfl:jibt.v1i1.1240.

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<p>Antioxidants can ward off free radicals in the body to resist oxidative damage caused by free radicals. The aim of this research is to know the influence of water solvent, methanol and methanol-water mixture to antioxidant activity. The extraction is done by maceration method. The extraction results in the phytochemical test and functional group analysis to determine the compounds contained in Pig leaf extract. Further testing of antioxidant activity with DPPH method. Pig leaf extract with methanol solvent has antioxidant activity with IC50 3,3005 μg / ml value, Pig leaf extract with

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Siskawati, Haeruddin, and Nurlansi. "Uji Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Daun Kelor (Moringa oleifera) Melalui Ekstraksi Maserasi." Sains: Jurnal Kimia dan Pendidikan Kimia 12, no. 1 (2023): 1–9. https://doi.org/10.36709/sains.v12i1.26.

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Phytochemical Tests and Antioxidant Activity of Moringa Leaf (Moringa oleifera) Methanol Extract Through Maceration Extraction Research has been carried out. This study aims to determine the secondary metabolites and antioxidant activity of Moringa Leaf Methanol Extract (Moringa oleifera). Secondary metabolite compounds contained in the ethanol extract of Moringa leaves were determined by the phytochemical method, while the antioxidant activity was determined by the DPPH method. Moringa leaves used are dried Moringa leaves that have been mashed. A total of 750 g of soni leaves were macerated f

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Khan, Wajid, Rizwan Ullah, Zainul Wahab, and Muhammad Nazir Uddin. "Antimicrobial effects of Verbascum thapsus L. leaf extracts." Bangladesh Journal of Botany 49, no. 4 (2020): 1119–25. http://dx.doi.org/10.3329/bjb.v49i4.52562.

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Antimicrobial activity of leaves of Verbascum thapsus L. using different extractions solvents was evaluated. Extracts were prepared and tested against different bacterial (Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus atrophaeus, Salmonella typhi, Citrobacter freundii and fungal species (Alternaria solani, Candida albicans, Aspergillus fumigatus). Disc and well diffusion assays were used for screening antibacterial and antifungal potential of extracts. The ethyl acetate extract produced the maximum zone of inhibition (70% ZI) against K. pneumoniae at 3000 μg/disc. Methanol extracts f

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K., Arunkumar, and K. R. Chandrashekar. "PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT STUDIES OF LEAF AND STEM BARK EXTRACTS OF POLYALTHIA FRAGRANS (DALZ.) BEDD.–AN ENDEMIC SPECIES OF WESTERN GHATS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 8 (2017): 20. http://dx.doi.org/10.22159/ijpps.2017v9i8.13915.

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Objective: Phytochemical constituents of various solvent extracts of leaf and stem bark of Polyalthia fragrans (Dalz.) Bedd were screened. Antimicrobial and antioxidant activities of the extracts were evaluated.Methods: Crude extracts of methanol, ethyl acetate, chloroform and aqueous of leaf and stem bark were evaluated for antimicrobial activity by disc diffusion method and antioxidant activity by DPPH (diphenyl-2-picrylhydrazyl) radical scavenging and reducing power assay. Quantitative analysis of total phenolics was done by the Folin-Ciocalteau method and total flavonoids by aluminum chlor

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Gavrilovic, Milan, Marina Sokovic, Milan Stankovic, Petar Marin, Zora Dajic-Stevanovic, and Pedja Janackovic. "Antimicrobial and antioxidative activity of various leaf extracts of Amphoricarpos vis. (Asteraceae) taxa." Archives of Biological Sciences 68, no. 4 (2016): 803–10. http://dx.doi.org/10.2298/abs160112068g.

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The antimicrobial and antioxidative activities of diethyl ether, 80% methanol and 50% acetone extracts of the leaves of three Amphoricarpos taxa (Asteraceae; A. neumayerianus, A. autariatus ssp. autariatus and A. autariatus ssp. bertisceus) from the Balkan Peninsula were investigated. The antimicrobial activity was determined by the broth microdilution assay against eight bacterial and eight fungal species. The in vitro antioxidative activity was assessed by the DPPH assay. The total phenolic and flavonoid contents were also determined. The most sensitive bacterial species were Bacillus cereus

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Afzal, Sheryar, Yuan Seng Wu, Chan Zelynn, and V. Appalaraju. "<i>In vitro</i> cytotoxic activity of <i>Sansevieria trifasciata</i> against various cancer cell lines." Tropical Journal of Pharmaceutical Research 23, no. 3 (2024): 491–99. http://dx.doi.org/10.4314/tjpr.v23i3.2.

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Purpose: To determine the in vitro anticancer properties of Sansevieria trifasciata extracts. Methods: The cytotoxic activity of n-hexane, ethyl acetate and methanol leaf extracts at different concentrations was assessed against Vero, pancreatic (AsPC-1), lung (A549), esophageal squamous carcinoma (KYSE 150) and rhabdomyosarcoma (RD) cancer cell lines using MTT assay. The apoptosis-inducing potential of selected S. trifasciata leaf extract was determined using a caspase 3/7 assay kit. Results: Ethyl acetate extract of S. trifasciata leaf showed significant anticancer activity against RD cells

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Awotedu, BF, TO Omolola, AO Akala, AO Ogunsiji, EA Owoeye, and SO Olaoti-Laaro. "Radical Scavenging activity and phytochemical screening of the leaf extract of Synsepalum dulcificum (Schumach. & Thonn.) Daniell." World Journal of Advanced Research and Reviews 10, no. 3 (2021): 069–76. https://doi.org/10.5281/zenodo.5066451.

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Antioxidants are chemicals that possess bioactive constituents which usually allow them to fight against free radicals. Antioxidant and phytochemicals properties of the leaves of <em>Synsepalum dulcificum</em> were investigated using ethyl-acetate, ethanol and methanol extracts. 2, 2-diphenyl-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, ferric reducing antioxidant power (FRAP), total flavonoid and the total phenolic content was carried out. The phytochemical screening assay was done on the following: Alkaloids, saponin, flavonoids, tannin, anthraquinones, te

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Salah, M. El-Sayed, and M. Said Saadiya. "Antioxidant and Insecticidal Effect of Some Plant Extracts against Callosbruchus maculates (coleoptera: Bruchidae)." Chemistry Research Journal 2, no. 3 (2017): 133–43. https://doi.org/10.5281/zenodo.13955288.

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The aim of the study was to test the effect of methanolic guava leaf and pomegranate peel extracts as antioxidant and insecticide. In the Gas Chromatography and Mass spectroscopy analysis, 30 bioactive phytochemical compounds were identified in the methanolic extract of guava leaf and 23 bioactive phytochemical compounds were identified, in the methanolic extract of pomegranate peel. The fraction for guava leaf methanol extract was characterized by large amounts of caryophyllene , β- humulene and longifolene, which constituted 16.42% , 15.74% and 12.13%  respectively , while the frac

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Biswas, Shashwata, Mohammad Shahriar, Jahan Ara Khanam, and Chowdhury Rafiqul Ahsan. "Investigation of Antioxidant, In-vitro Cytotoxic, and In-vivo Antitumor Effects of Leaf Extracts of Annona Reticulata." Bangladesh Journal of Microbiology 29, no. 2 (2016): 70–74. http://dx.doi.org/10.3329/bjm.v29i2.28439.

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This study was carried out to evaluate the antioxidant, brine shrimp lethality, bioassay and antitumor effects of nhexane, petroleum ether, methanol and chloroform extracts of Annona reticulata leaves. All extracts demonstrated good cupric reducing activity with increasing concentrations keeping methanol extract at the top and n-hexane extract at the second position. The methanol extract was found to have the highest antioxidant capacity 28±26.88 followed by n-hexane 16.95±11.39 and chloroform 12.85±10.32 in the form of mg of ascorbic acid equivalent per gram of crude extract. The methanol ext

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Omidiwura, B. R. O. "Qualitative and quantitative analysis of pawpaw (Carica papaya) leaf extract and its antimicrobial effect in animal production." Nigerian Journal of Animal Production 44, no. 3 (2021): 76–83. http://dx.doi.org/10.51791/njap.v44i3.746.

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In order to improve livestock production and curb the losses from diseases occurrence in livestock, producers resort to the use of antimicrobials as growth promoters to inhibit the growth of disease-causing organisms. Freshly harvested pawpaw leaves were extracted using three solvents: ethanol, methanol and n-hexane and their phytochemicals determined using standard procedure. The inhibitory activities of the extracts at low (200ppm) and high (1000ppm) concentrations against Aspergillus niger and Escherichia coli were also determined. Results obtained showed that alkaloid, flavonoid, saponin,

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Enweani-Nwokelo, I. B., E. U. Urama, and N. O. Achukwu. "Evaluation of the antimicrobial activities of Napoleona imperialis leaf extracts against multi-drug resistant bacterial isolates from diabetic foot ulcer." African Journal of Clinical and Experimental Microbiology 26, no. 1 (2024): 73–80. https://doi.org/10.4314/ajcem.v26i1.9.

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Background: The increased resistance of Gram-negative bacteria, particularly those that produce extended beta-lactamase, are limiting the efficacy of antimicrobial drugs in treating infected diabetic foot ulcers. This study evaluates antimicrobial activities of Napoleona imperialis leaf extracts on bacterial isolates of diabetic foot ulcers as a way of developing novel antimicrobials that will be effective in treating infections caused by multi-drug-resistant (MDR) bacterial isolates. Methodology: Fresh leaves of N. imperialis were collected and identified by a plant taxonomist at the Departme

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Flaifel, Mustafa Khaled, Haitham Qaralleh, Saif M. Dmour, Moath Alqaraleh, and Aya Yassin Al-Kabariti. "Azadirachta indica (Neem) Leaf: chemical composition and antibacterial activity as determined by different polarity solvents." Research Journal of Biotechnology 18, no. 2 (2023): 55–62. http://dx.doi.org/10.25303/1802rjbt55062.

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The aim of this study was to investigate the antibacterial activity of Azadirachta indica leaf extracts against Staphylococcus aureus, Escherichia coli, Streptococcus pneumonia and Proteus mirabilis. This was performed using the disc diffusion method and the microdilution method (MIC). Solvents of variable polarity were used to extract the components of A. indica. The highest yield of extraction was obtained from methanol (3.30%), followed by ethanol (3.00%), ethyl acetate (1.50%) and hexane (0.90%). In general, A. indica leaf extracts exhibited broad spectrum antibacterial activity. The extra

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Silalahi, Mario, Yohana Sutiknyawati Kusumadewi, and Henny Sulistyowati. "PENGARUH EKSTRAK FENOLIK ANTIFOTOOKSIDAN DAUN KESUM (Polygonum minus) TERHADAP STABILITAS EMULSI PADA MINYAK RBDPO (REFINED BLEACHED DEODORIZED PALM OIL ) DALAM AIR." Perkebunan dan Lahan Tropika 12, no. 1 (2022): 15. http://dx.doi.org/10.26418/plt.v12i1.60019.

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This study aimed to obtain the type of solvent that has optimal activity antiphotooxidaion to inhibit photooxidation RBDPO oil, and determine the effect of phenolic extracts of the kesum leaf on RBDPO oil emulsion stability.The study consisted of two steps. The first steps make kesum leaf extract with methanol, n-hexane fraction, ethyl acetate fraction, the fraction of methanol and methanol-water fraction.Each extract with a concentration of 1000 ppm RBDPO emulsified into the oil, then proceed with testing antiphotooxidation activity and total phenol content using a completely randomized desig

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Prithviraj H.S, Hemanth Kumar N.K, Basavaraj G.L, Nataraj K, and Shobha Jagannath. "Preliminary phytochemical analysis and antibacterial activity of leaf and leaf derived callus extracts of Passiflora caerulea: An important medicinal plant." World Journal of Advanced Research and Reviews 17, no. 1 (2023): 203–9. http://dx.doi.org/10.30574/wjarr.2023.17.1.0009.

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In the current study the Preliminary phytochemical analysis and antibacterial activity of leaf and leaf derived callus extracts of Passiflora caerulea were carried out in different solvent extracts.MS medium showed a better response than B5 medium and White’s medium for callus induction. The plant and callus extracts showed the presence of carbohydrates, tannins and glycosides and leaf extracts showed the presence of carbohydrates, whereas stem extracts showed the presence of carbohydrates and phlobatannins. . The methanol, chloroform, leaf extract showed an inhibition zone of 8.00 mm against

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Attar, Usmangani A., and Savaliram G. Ghane. "PHYTOCHEMICALS, ANTIOXIDANT ACTIVITY AND PHENOLIC PROFILING OF DIPLOCYCLOS PALMATUS (L.) C. JEFFERY." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 4 (2017): 101. http://dx.doi.org/10.22159/ijpps.2017v9i4.16891.

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Objective: The aim of this study was to analyze phytochemicals, antioxidant potential and phenolic profiling of leaf and fruit extracts of Diplocyclos palmatus.Methods: The leaves and fruits were subjected for sequential extraction with hexane, chloroform, methanol and water. All extracts were subjected to biochemical studies such as phenols, tannins, flavonoids, terpenoids and antioxidant assays such as 1,1-diphenyl-1-picryl hydrazyl (DPPH), 2,2’ Azinobis (3-ethyl-benzothiozoline-6-sulfonic acid (ABTS), ferric reducing antioxidant property (FRAP), metal chelating and phospho- molybdenum reduc

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Mostafa, M., S. Ahmed, and AJ Afolayan. "Antioxidant activity of Clematis brachiata Thunb. leaf." Bangladesh Journal of Scientific and Industrial Research 53, no. 3 (2018): 185–90. http://dx.doi.org/10.3329/bjsir.v53i3.38264.

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The antioxidant activity, total phenolic and flavonoid contents of different extracts of the Clematis brachiata Thunb leaves were determined. The antioxidant activity was evaluated using spectroscopic methods against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2′-azinobis [3-ethylbenzothiazoline-6-sulphonic acid] diammonium salt radical cation (ABTS). Folin Ciocalteu method was used to determine the total phenolics and Aluminium Chloride Colorimetric method was used to determine the flavonoids contents in these extracts. The results showed that the methanol extract of the leaf exhibited

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TAŞKAYA, Aylin, Burcu ŞAHİN, GÜVENSEN Nur CEYHAN, and Ramazan MAMMADOV. "A Preliminary Study on Anticancer and Antimicrobial Potential of Methanolic Extracts of Verbascum napifolium." ISPEC Journal of Agricultural Sciences 7, no. 1 (2023): 135–45. https://doi.org/10.5281/zenodo.7749361.

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Plants are widely used in the treatment of many diseases. This study evaluates for the first time the potential anticancer and antimicrobial activities of methanol extracts of leaves and flower parts of <em>Verbascum napifolium</em>, an endemic species distributed in the flora of Turkey. The antimicrobial activities were evaluated by the disc diffusion method and by determining the minimal inhibitory concentration (MIC). In all bacteria, flower methanol extract was found to be more sensitive. Escherichia coli was determined to be more sensitive to both extracts compared to all tested bacteria.

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Guleria, Dimple, Amit Kumar Sehgal, and Anand Sagar. "Antibacterial, Antioxidant Activities and Phytochemical Constituents of Jatropha curcas L. Leaves." Journal of Plant Science Research 38, no. 2 (2023): 637–50. http://dx.doi.org/10.32381/jpsr.2022.38.02.19.

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The study was carried out for investigation of antibacterial and antioxidant activities along with phytochemical analysis of leaf extracts of Jatropha curcas L. Extraction was done by using methanol and acetone solvents. Antibacterial activity was analysed by Agar well diffusion method against four human pathogenic bacteria, which included two Gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Streptomycin was taken as a standard and positive control. Both methanol and acetone leaf extracts sho

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More, Keshav Kalyan, Rajendra Maratrao Gade, Nitin Vitthal Gurav, and Rupeshkumar Jagannath Chaudhari. "Phytochemical Investigation and Thin Layer Chromatography of Methanol Extract of Psoralea corylifolia and Emblica officinalis Leaves." International Journal of Plant & Soil Science 35, no. 19 (2023): 653–63. http://dx.doi.org/10.9734/ijpss/2023/v35i193595.

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The current study focuses on phytochemical screening of Psoralea corylifolia and Emblica officinalis leaf extracts in methanol solvent. The antibacterial compounds found in both leaf extracts of Psoralea corylifolia and Emblica officinalis plants were investigated using phytochemical studies. The extracts contained flavonoids, terpenoids, tannins, alkaloids, saponins, and phenolic chemicals, according to preliminary phytochemical screening. The solvent systems of toluene: ethyl acetate: methanol (24:5:2) and chloroform: ethyl acetate: acetic acid (50:50:1) yielded the most phytochemicals from

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