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Dissertations / Theses on the topic 'Microsponge'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Guyon, Virginie Noelle Veronique. "Molecular study of microspore development in Brassica napus." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387037.

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2

Triggs, Heidi M. "Haploid production and genetic transformation of wheat (Triticum aestivum L.)." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243689.

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3

Zhao, Jiping. "Induction and mechanism of Brassica napus cv. Topas microspore embryogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq20600.pdf.

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4

Ili´c-Grubor, Katica. "A novel approach to microspore embryogenesis in Brassica Napus L." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ32787.pdf.

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5

Stadnyk, Kimberly D. "Improvement on the microspore culture methodology for Brassica rapa canola." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ41780.pdf.

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6

Daniels, Stephen J. "Studies in the production of microspore-derived haploids in lupin." Thesis, University of Reading, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266148.

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7

Camacho, Fernández Carolina. "Microspore embryogenesis: cell wall dynamics and reprogramming of cell fate." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/163698.

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[ES] Los dobles haploides son una gran herramienta para la mejora genética de híbridos debido a que se puede alcanzar la homocigosis completa en una sola generación. Entre las técnicas usadas para obtener estas plantas, la inducción de la embriogénesis de microsporas, mediante el cultivo de anteras o de microsporas, es la más eficiente y la más usada. La embriogénesis de microsporas es también un ejemplo de totipotencia de las células vegetales gracias a su habilidad de reprogramar su desarrollo gametofítico hacia una ruta esporofítica, donde las células proliferan de forma organizada para cre

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8

Bélanger, Sébastien. "Caractérisation génomique et transcriptomique de la microspore embryogénique chez l'orge." Doctoral thesis, Université Laval, 2019. http://hdl.handle.net/20.500.11794/33304.

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L’androgenèse est une biotechnologie végétale utilisée pour fixer le bagage génétique des plantes en une seule génération. Elle repose sur la capacité d’un grain de pollen immature, la microspore, à restaurer sa totipotence cellulaire végétale et se dédifférencier puis s’engager dans la voie de l’embryogenèse. Or, on observe que la capacité de la microspore à s’engager dans l’embryogenèse est génétiquement variable. En dépit des nombreux avantages qu’elle présente, l’androgenèse entraîne souvent une conséquence indésirable, soit une distorsion de la ségrégation (DS) chez les populations issues

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9

Oberholzer, Ian Dewald. "Peroral and nasal delivery of insulin with PheroidTM technology / Ian D. Oberholzer." Thesis, North-West University, 2009. http://hdl.handle.net/10394/4964.

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Since its initial discovery in 1922 by Banting and Best, the formulation of an oral insulin delivery system has ever been so troublesome. Unfortunately, insulin is indispensable in the treatment of diabetes mellitus, which affects approximately 350 million people worldwide. Various factors contribute to the peptide being such a persistently difficult hormone to be used in an oral formulation. The gastrointestinal tract is home to various protein digestive enzymes such as pepsins in the stomach and trypsin, chymotrypsin and carboxypeptidases in the small intestine, which digests insulin. Also t

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10

Cimò, Giuseppe. "Ploidy manipulation for genetic improvement in some Mediterranean fruit crops." Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/79874.

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Plant breeding is focused on selection of new genotypes with improved traits. Conventional methods based on hybridization and those based on biotechnology (somatic hybridization, genetic transformation, ploidy manipulation, etc.) are used to create novel genetic variations. Biotechnology provides powerful tools for plant breeding, for instance, haploid technology allows achievement of homozygous lines from heterozygous parents in one step, which reduces significantly the time required by conventional methods. Concerning woody species, characterized by self-incompatibility, long juvenile period

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11

Redha, Amina. "Induction and analysis of chromosome doubling of microspore derived wheat haploids /." Zürich, 1998. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=12855.

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12

Boutilier, Kim. "Isolation and characterization of molecular markers for Brassica napus microspore embryogenesis." Thesis, University of Ottawa (Canada), 1994. http://hdl.handle.net/10393/6474.

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Brassica napus microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. This switch in developmental pathways has been shown to be accompanied by the induction of high levels of napin seed storage protein gene expression (DeMoor, 1992). Specific members of the napin multigene family that were expressed at this time were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that,

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13

Furukawa-Stoffer, Tara Lee. "Characterization of triacylglycerol biosynthetic enzymes from microspore-derived cultures of oilseed rape." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0027/MQ38426.pdf.

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14

Furukawa-Stoffer, Tara L., and University of Lethbridge Faculty of Arts and Science. "Characterization of triacylglycerol biosynthetic enzymes from microspore-derived cultures of oilseed rape." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1996, 1996. http://hdl.handle.net/10133/63.

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Particulate and solubilized preparations of phosphatidate (PA) phosphatase (EC 3.1.3.4) and dia-cylglycerol acyltransferase (DGAT, EC 2.3.1.20) from microspore-derived (MD) cultures of Brassica napus L. cv Topas were characterized. The activity of solubilized PA phosphatase decreased by about 50% following storage for 24 h at 4 degrees celsius, whereas the activity of DGAT decreased by 30%. Bovine serum albumin increased the stability of both enzymes. Both preparations were enriched in the target enzyme and thus, may be useful in studies of regulation with limited influence by the other Kenned

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15

Tratt, Julia. "Identifying the wall-degrading enzymes responsible for microspore release from the pollen tetrad." Thesis, University of Bath, 2016. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687381.

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Dissolution of the pollen tetrad walls after meiosis, and subsequent release of the haploid microspores, is essential for pollen development in many species, including Arabidopsis thaliana. The thick wall of the tetrad consists of callose (β-1,3-glucan), with a primary cell wall of cellulose, hemicellulose and pectin surrounding the whole structure. Despite the importance of microspore release, not all the wall-degrading enzymes involved have been identified in A. thaliana. 18 endo-β-1,3-glucanase genes were identified in A. thaliana that are expressed in buds at microspore release. Single and

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16

Kocsis, Michael G., and University of Lethbridge Faculty of Arts and Science. "Characteristics of phosphatidate phosphatase from developing seeds and microspore-derived cultures of oilseed rape." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1994, 1994. http://hdl.handle.net/10133/23.

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Phosphatidate phosphatase (PAP. EC 3.1.3.4) was charaterized from developing seeds and microspore-derived (MD) cultures of oilseed rape. In studies with homogenate from developing seeds (Brassica napus L. cv Westar) the time course for release of inorganic phosphate from phosphatidate was linear for at least 60 min and the enzyme was stable to at least three cycles of freezing and thawing. Differential centrifugation studies were conducted with homogenate prepared from developing seeds (B. napus L. cv Westar), MD embryos (B. napus L. cv Reston), and an embryogenic MD cell suspension culture (B

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17

Kotzé, Jeanéne Celesté. "Nasal drug delivery of calcitonin with pheroid technology / Jeanéne Celesté Kotzé." Thesis, North-West University, 2005. http://hdl.handle.net/10394/888.

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Advances in biotechnology and recombinant technologies have lead to the production of several classes of new drugs such as peptide and protein drugs. These compounds are mostly indicated for chronic use but their inherent characteristics such as size, polarity and stability prevent them from incorporation in novel dosage forms. The bioavailability of nearly all peptide drugs is very low due to poor absorption from the administration site. Several challenges confront the pharmaceutical scientist in developing effective and innovative dosage forms for these classes of drugs. A lot of attention h

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18

Byers, Susan D. "Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/MQ49143.pdf.

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19

Byers, Susan D., and University of Lethbridge Faculty of Arts and Science. "Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1999, 1999. http://hdl.handle.net/10133/101.

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Several factors including an unidentified endogenous substance were found to stimulate microsomal diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) from a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv Jet Neuf). Mg2+ salts were found to stimulate microsomal DGAT 14 to 23-fold. ATP and CoA were also found to stimulate the enzyme 2.4 and 12 fold respectively, although the effects were decreased in the presence of high Mg2+ concentrations. While microsomal DGAT activity was only slightly increased by the concentration of exogenous diacylglycerol in the reaction

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20

MOHAMED, ABDELGALEL MOHAMED Ahmed. "STUDY ON GAMETIC EMBRYOGENESIS VIA IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE IN FRUIT CROPS." Doctoral thesis, Università degli Studi di Palermo, 2014. http://hdl.handle.net/10447/91257.

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Fruit breeding is mainly based on both conventional (hybridization, mutation and selection) or biotechnological methods (somatic hybridization, genetic transformation and haploid production). The genetic improvement through the conventional methods is limited by many factors such as fruit trees long juvenile period, high heterozygosity, large size and sexual incompatibility. Haploids and doubled haploids, obtained through gametic embryogenesis have a potential use in fruit crops genetic improvement. The change of the microspores fate from the normal gametopytic pathway towards the sporophytic

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21

De, Bruyn Tanile. "Nasal delivery of insulin with Pheroid technology / Tanile de Bruyn." Thesis, North-West University, 2006. http://hdl.handle.net/10394/730.

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Approximately 350 million people worldwide suffer from diabetes mellitus (DM) and this number increases yearly. Since the discovery and clinical application of insulin in 1921, subcutaneous injections have been the standard treatment for DM. Because insulin is hydrophilic and has a high molecular weight and low bioavailability, this molecule is poorly absorbed if administered orally. The aim of this study is to evaluate nasal delivery systems for insulin, using Sprague Dawley rats as the nasal absorption model. Pheroid technology and N-trimethyl chitosan chloride (TMC) with different dosages o

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22

Davoren, Jonathan M., and University of Lethbridge Faculty of Arts and Science. "Gene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil production." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997, 1997. http://hdl.handle.net/10133/345.

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Triacylglycerol (TAG) production in the microspore derived (MD) cell suspension culture ofBrassica napus L. cv Jet Neuf was enhanced when the sucrose concentration in the growth medium was increased from 2 to 14 % (w/v). mRNA differential display by polymerase chain reaction was used to examine gene expression in cells grown at different sucrose concentrations in order to identify mRNAs which could be associated with oil formation. The anchored primer, T12AA, was used to screen one subset, representing approximately one twelfth of the transcript population, isolated from cultures grown in medi

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23

Rivas, Sendra Alba. "Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant." Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/85548.

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Androgenesis induction is an experimental procedure by which microspores are diverted from their original gametophytic pathway towards embryogenesis by applying specific stresses in vitro. It allows for the production of doubled haploid (DH) pure lines through anther culture or isolated microspore culture followed by chromosome doubling. DH technology is interesting for both basic research and plant breeding. In this Thesis, we studied microspore embryogenesis with two parallel approaches: (I) an applied study directed to the development of the first eggplant (Solanum melongena) highly embryog

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24

Pérez-Piñar, López Teresa [Verfasser]. "Study of the metabolism during microspore embryogenesis in wheat DH lines using -omics approaches / Teresa Pérez-Piñar López." Halle, 2019. http://d-nb.info/1181097436/34.

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25

Howley, Paul Michael. "The cloning of microsporogenesis and meiosis specific sequences from the meiocyte, microspore and pollen cells of Lilium henryii." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332857.

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26

Steyn, Johan Dewald. "Nasal delivery of recombinant human growth hormone with pheroid technology / Dewald Steyn." Thesis, North-West University, 2006. http://hdl.handle.net/10394/1276.

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Over the past couple of years there has been rapid progress in the development and design of safe and effective delivery systems for the administration of protein and peptide drugs. The effective delivery of these type of drugs are not always as simple as one may think, due to various inherent characteristics of these compounds. Due to the hydrophilic nature and molecular size of peptide and protein drugs, such as recombinant human growth hormone, they are poorly absorbed across mucosal epithelia, both transcellularly and paracellularly. This problem can be overcome by the inclusion of absorpt

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27

Gervais, Carmen. "Actin filament organization and function in pollen development and induction of microspore embryogenesis of Brassica napus L. cv. Topas." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ42943.pdf.

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28

Peng, Xiaobing Carleton University Dissertation Biology. "The role of abscisic acid and abscisic acid-analogs in inducing desiccation tolerance in microspore-derived embryos of Brassica Napus." Ottawa, 1994.

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29

Elahi, Nosheen. "The potential of using the BnLEC1 and BnFUSCA3 genes to manipulate oil content in Brassica napus L." Elsevier-Plant Physiology and Biochemistry, 2015. http://hdl.handle.net/1993/31841.

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Due to the immense utilization in food and industry, there is enormous commercial and scientific interest to manipulate canola (Brassica napus L.) seed oil. Seed oil accretions are influenced by genes involved in embryo and seed development. FUSCA3 (FUS3) and LEAFY COTYLEDON1 (LEC1) are well-known transcription factors involved during seed and embryo development. The main objective of this project was to evaluate the role of these genes during seed storage deposition and microspore-derived embryogenesis in B. napus. For this purpose, six BnLEC1 transgenic lines and three BnFUS3 TILLING mutant

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30

Cima, Francieli Fatima. "Otimização de protocolo para produção de plantas duplo-haploides através da cultura de micrósporos isolados de trigo." Universidade Federal de Pelotas, 2014. http://repositorio.ufpel.edu.br:8080/handle/prefix/3063.

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Submitted by Gabriela Lopes (gmachadolopesufpel@gmail.com) on 2016-09-27T14:35:10Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertação_Francieli_Cima.pdf: 1564850 bytes, checksum: 1875a0cdb8f46c6a1880c860e06bdbe3 (MD5)<br>Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2016-09-28T17:12:22Z (GMT) No. of bitstreams: 2 Dissertação_Francieli_Cima.pdf: 1564850 bytes, checksum: 1875a0cdb8f46c6a1880c860e06bdbe3 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)<br>Made available in DSpace on 20

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Klutschewski, Sarah [Verfasser], Christian [Akademischer Betreuer] Möllers, Traud [Akademischer Betreuer] Winkelmann, and Petr [Akademischer Betreuer] Karlovsky. "Methodical improvements in microspore culture of Brassica napus L. = Methodische Verbesserungen in der Mikrosporenkultur von Brassica napus L. / Sarah Klutschewski. Gutachter: Christian Möllers ; Traud Winkelmann ; Petr Karlovsky. Betreuer: Christian Möllers." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://d-nb.info/1044870249/34.

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32

Valdés, Valázquez Ariana Istar [Verfasser], Christian [Akademischer Betreuer] [Gutachter] Möllers, and Petr [Gutachter] Karlovsky. "Inheritance of microspore embryogenic potential and direct embryo to plant conversion in the oilseed rape DH population DH4079 x Express 617 / Ariana Istar Valdés Valázquez ; Gutachter: Christian Möllers, Petr Karlovsky ; Betreuer: Christian Möllers." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1132336791/34.

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33

Kuprienė, Ramunė. "Geltonsėklių vasarinių rapsų (Brassica napus L.) kūrimas biotechnologiniais ir tradiciniais selekcijos metodais." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20061121_110825-64679.

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Genotypes of rapeseeds producing yellow seeds were not found in nature. Breeders yellow-seeded rapeseeds have been developed applying different combinations of interspecific crosses. In the Laboratory of Genetics-Biotechnology at the Lithuanian Agricultural University, yellow-seeded spring rapeseeds were developed for the first time without interspecific crosses (Burbulis, 2001). All cultivars of yellow-seeded rapeseed have one essential drawback – unblocking of pigmentation takes place in other generations and seeds of different colours (yellowish brown, brown or black) are formed. Breeders,

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34

"Induction of microspore embryogenesis in Brassica Napus Via anther culture and isolated microspore culture." Adelaide : Thesis (M.Ag.Sc.) -- University of Adelaide, Waite Agricultural Research Institute, 1991. http://web4.library.adelaide.edu.au/theses/09A/09al7835.pdf.

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35

Liu, Qing. "The induction of microspore embryogenesis in Brassica napus Via anther culture and isolated microspore culture." Thesis, 1991. http://hdl.handle.net/2440/110193.

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36

Klutschewski, Sarah. "Methodical improvements in microspore culture of Brassica napus L." Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BC20-2.

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Bei der routinemäßigen Anwendung der Mikrosporenkultur zur Herstellung doppelt-haploider Linien kommt es bis heute zu Engpässen in der praktischen Rapszüchtung. Die Hauptprobleme stellen eine unzureichende Colchizin-induzierte Diploidisierungsrate und eine niedrige direkte Regeneration von Pflanzen aus Mikrosporen-Embryonen dar. Ein hoher Prozentsatz an Rapsembryonen aus Mikrosporenkultur durchläuft den Prozess der sekundären Embryogenese, der eine zeitintensive Subkultivierung erfordert. Hierbei werden die direkten Sprossansätze wiederholt von undifferenziertem Gewebe freigeschnitten bis eine

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37

Stadnyk, Kimberly Dawn. "Improvement on the microspore culture methodology for Brassica rapa canola." 1999. http://hdl.handle.net/1993/7327.

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The objective of this research is to improve the microspore culture methodology based on embryo production levels for B. rapa. An efficient isolated microspore culture methodology for Brassica rapa canola could be used by breeding programs to produce high, consistent embryo yields. The project was divided into several experiments looking at modifications to the culture media, microspore pretreatments, microspore liberation techniques and donor plant pretreatments. Four B. rapa genotypes were selected for the experiments: Reward, AC Parkland, AC-1 and AC-2. The standard protocol used in this re

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38

Ladeira, Bruno Miguel Fernandes. "Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction." Master's thesis, 2021. http://hdl.handle.net/10773/32992.

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As the leading cause of death worldwide, myocardial infarction (MI) carries considerable socioeconomic costs. The damage and clinical complications caused by MI remain unaddressed by current therapeutic strategies. With the advent of tissue engineering and regenerative medicine, novel strategies to promote regeneration of heart muscle are possible. This dissertation focuses on the application of microcapsule-based systems to promote the recovery of damaged tissue. The biofabrication of core-shell microcapsules for the encapsulation of cells and bioactive molecules has been reviewed, al

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Chen, Yen-Chou, and 陳嬿州. "Isolation, Purification and Cultivation of Microspore of Rice (Oryza sativa L.)." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/62941822422614398834.

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碩士<br>國立中興大學<br>生命科學系所<br>95<br>Production of doubled-haploid plants via microspore embryogenesis is an effective means in crop improvement breeding in search of Novel traits. In this study, four rice ( Oryza sativa L. ) cultivars were used to evaluate the possibility of generating rice plants from microspores. Culture medium composition, culture method, ovary nurse culture were studied to improve the efficiency of doubled-haploid plant production. Microspores in mid- to late-uninucleate stage were isolated from four cultivars, ‘Taikeng 8’、‘Taichung 65’、‘Tainung 67’ and ‘SA 0420’ by blending o

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40

Chang, Zao-Yen, and 張詔雁. "Characterization and function analysis of a tapetum/microspore-specific gene in Lilium longiforum." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/87511205740085610131.

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碩士<br>國立中興大學<br>生物科技學研究所<br>102<br>LLA139 transcript was identified from a suppression subtractive cDNA library at the microspore satge of lily (Lilium longiflorum) anthers. The LLA139 cDNA encodes 102 amino acids. Taking out the signal peptide of 30 amino acids at the N-terminus, the estimated molecular mass of mature LLA139 protein is 7.3 kDa. The protein may form a structure of two finger motifs. Sequence analysis indicates that the protein shares 42% and 47% identity with LLA67 and HvLEM1 protein, respectively. Particularly the cysteine residues in the sequence of these proteins are highly

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Lin, Bo-Ying, and 林柏穎. "Application of Impedance Flow Cytometry in Microspore Culture of Brassica oleracea var. botrytis L." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/rdb983.

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碩士<br>國立中興大學<br>園藝學系所<br>107<br>Brassica oleracea var. botrytis L. is one of the most important vegetables in the world. Nowadays, the microspore culture technique is wildly and efficiently used in Brassica to produce double haploid (DH) for breeding elite F1 hybrids. In order to improve the production efficiency of DH, selection of microspores at the suitable developmental stages and detection of the microspore responses during culturing process are important for the generation of DH. However, the lack of an efficient analyzing instrument remains a problem. Flower buds of three F1 cultivars o

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42

Belmonte, Mark Findlay. "Glutathione modulation of in vitro organized development in canola microspore derived and spruce somatic embryos." 2008. http://hdl.handle.net/1993/8034.

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The production of good quality microspore derived embryos (MDEs) of Brassica napus cv. Topaz (canola) and somatic embryos of spruce including Picea glauca and P. abies (white spruce and Norway spruce, respectively) are of great importance for developmental and applied studies. One of the obstacles encountered during the culture process is the lack of organized developmet, especially within the shoot apical meristem (SAM). In the last few years, glutathione and its association in the ascorbate-glutathione cycle has been viewed as rational modulator of embryo development in vivo and in vitro. I

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Huang, Shih-Yu, and 黃詩鈺. "Exploring gene expression and stability of foreign gene by microspore embryogenesis in the transgenic rice." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/90737347574322596000.

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碩士<br>國立中興大學<br>生命科學系所<br>96<br>The stability of genes is very important for genetic traits. In this study,a CK transgenic rice which overexpresses two maize C4 photosynthesis genes encoding phosphoenolpyruvate carboxylase(PEPC) and pyruvate Pi dikinase(PPDK) was used to test the stability and expression level of these two transgenes in dihaploid line derived from microspore embryogenesis. A tatal of thiry-two dihaploid lines were obtained for molecular, biochemical and physiological analysis related to the two genes. Western immuno-blot analysis showed that all lines have high levels of PPDK

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Valdés, Velázquez Ariana Istar. "Inheritance of microspore embryogenic potential and direct embryo to plant conversion in the oilseed rape DH population DH4079 x Express 617." Doctoral thesis, 2016. http://hdl.handle.net/11858/00-1735-0000-0023-3E44-8.

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Lin, I.-Pei, and 林依霈. "Studies on anther and microspore cultures and root tip karyotype examination of purple coneflower(Echinacea purpurea L.)." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/59440233745586531594.

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碩士<br>國立中興大學<br>農藝學系所<br>97<br>Purple coneflower (Echinacea purpurea L.) is one of the most popular health protection herbs recently and it is a cross-pollinated crop with dominant self-incompatibility. Considering the heavy burden of purification of inbred line via self-pollination in a long schedule, we tried to employ anther culture to get doubled-haploid plants. To promote efficiency the microspore culture was studied for avoiding paying attention to dissect where the regenerated plants were derived from. Furthermore, karyotype examination protocol was developed to aim at coneflower plantl

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Urban, Milan. "Fyziologická a proteomická charakterizace vlivu abiotických stresů na ozimou formu brukve řepky olejky." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-356117.

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- Ph.D. thesis - Milan Urban, 2017 In some years, the agricultural production of oilseed rape, an important crop in the Czech Republic, is - besides biotic stress - facing the problem of damage caused by frost or drought. Together with special attention paid to proteins revealing responses between crop genotypes with differential abiotic stress tolerance levels we reviewed possible applications of proteomic results in crop breeding programs aimed at an improvement of crop stress tolerance (paper 1). For first original result, cold temperature was imposed upon non-vernalized plants in the stage

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