Academic literature on the topic 'Morganii 16s rdna analysis'

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Journal articles on the topic "Morganii 16s rdna analysis"

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Tabassum, Anika, Mihir Lal Saha, and Mohammad Nurul Islam. "Prevalence of multi-drug resistant bacteria in selected street food and water samples." Bangladesh Journal of Botany 44, no. 4 (2018): 621–27. http://dx.doi.org/10.3329/bjb.v44i4.38599.

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Present study was conducted to determine the bacteria and their multi-drug resistance pattern of Velpuri and water of Velpuri shop of different areas of Dhaka city. A total of 74 bacteria were isolated of which 26 isolates were subjected for further study. Eleven and 15 isolates from 26, were found Gram-positive and Gram-negative bacteria, respectively. Three isolates of Gram-positive bacteria were found rod shaped and spore formers which were identified as Bacillus spp. while eight isolates were found round shaped and nonspore formers and identified as Staphylococcus, Streptococcus, Planococc
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Maes, Michael, Asara Vasupanrajit, Ketsupar Jirakran, et al. "Exploration of the Gut Microbiome in Thai Patients with Major Depressive Disorder Shows a Specific Bacterial Profile with Depletion of the Ruminococcus Genus as a Putative Biomarker." Cells 12, no. 9 (2023): 1240. http://dx.doi.org/10.3390/cells12091240.

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Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in the microbiota–gut–brain axis, as evidenced by elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative bacteria, such as Morganella morganii and Klebsiella Pneumoniae. The latter aberrations, which point to increased gut permeability (leaky gut), are linked to activated neuro-immune and oxidative pathways in MDD. To delineate the profile and composition of the gut microbiome in Thai patients with MDD, we examined fecal samples of 32 MDD patients and 37
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KIM, SHIN-HEE, HAEJUNG AN, KATHARINE G. FIELD, et al. "Detection of Morganella morganii, a Prolific Histamine Former, by the Polymerase Chain Reaction Assay with 16S rDNA–Targeted Primers." Journal of Food Protection 66, no. 8 (2003): 1385–92. http://dx.doi.org/10.4315/0362-028x-66.8.1385.

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A polymerase chain reaction (PCR) assay for the rapid and sensitive detection of the most prolific histamine former, Morganella morganii, was developed.16S rDNA targeted PCR primers were designed, and the primer specificity and sensitivity of the PCR assay were evaluated. The 16S rDNA sequence (1,503 bp) for M. morganii showed 95% identity to those for enteric bacteria, i.e., Enterobacter spp., Klebsiella spp., Citrobacter spp., Hafnia alvei, Proteus spp., and Providencia spp. The unique primers for M. morganii were designed on the basis of the variable regions in the 16S rDNA sequence. The pr
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Dahllöf, Ingela, Harriet Baillie, and Staffan Kjelleberg. "rpoB-Based Microbial Community Analysis Avoids Limitations Inherent in 16S rRNA Gene Intraspecies Heterogeneity." Applied and Environmental Microbiology 66, no. 8 (2000): 3376–80. http://dx.doi.org/10.1128/aem.66.8.3376-3380.2000.

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ABSTRACT Contemporary microbial community analysis frequently involves PCR-amplified sequences of the 16S rRNA gene (rDNA). However, this technology carries the inherent problem of heterogeneity between copies of the 16S rDNA in many species. As an alternative to 16S rDNA sequences in community analysis, we employed the gene for the RNA polymerase beta subunit (rpoB), which appears to exist in one copy only in bacteria. In the present study, the frequency of 16S rDNA heterogeneity in bacteria isolated from the marine environment was assessed using bacterial isolates from the red alga Delisea p
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Lamy, Brigitte, Fréderic Laurent, and Angeli Kodjo. "Validation of a partialrpoBgene sequence as a tool for phylogenetic identification of aeromonads isolated from environmental sources." Canadian Journal of Microbiology 56, no. 3 (2010): 217–28. http://dx.doi.org/10.1139/w10-006.

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A collection of 50 aeromonads isolated from environmental sources were studied, together with all known Aeromonas nomenspecies, by phenotypic, amplified 16S rDNA restriction analysis (16S rDNA RFLP) and by partial sequence alignment of both 16S rDNA and rpoB genes. Although most of the type strain showed a unique phenotypic pattern, a database constructed on type strain phenotype allowed the identification of only 24% of the isolates. Analysis of 16S rDNA RFLP and the rpoB sequence were almost concordant in identifying environmental isolates at the species level, except for strains belonging t
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Emborg, Jette, Paw Dalgaard, and Peter Ahrens. "Morganella psychrotolerans sp. nov., a histamine-producing bacterium isolated from various seafoods." International Journal of Systematic and Evolutionary Microbiology 56, no. 10 (2006): 2473–79. http://dx.doi.org/10.1099/ijs.0.64357-0.

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Mesophilic Morganella morganii (n=6) and psychrotolerant M. morganii-like isolates from various seafoods (n=13), as well as clinical M. morganii isolates (n=3), were characterized by using a polyphasic approach including multi-locus sequencing. Based on the phylogenetic analysis, the 22 strains were divided into two distinct groups comprising mesophilic and psychrotolerant isolates, respectively. This classification was supported by DNA–DNA hybridization studies, whereby a psychrotolerant isolate (strain U2/3T) showed 41.0 and 17.8 % relatedness to the type strains of the mesophilic species Mo
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Drancourt, Michel, Claude Bollet, Antoine Carlioz, Rolland Martelin, Jean-Pierre Gayral, and Didier Raoult. "16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates." Journal of Clinical Microbiology 38, no. 10 (2000): 3623–30. http://dx.doi.org/10.1128/jcm.38.10.3623-3630.2000.

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Some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. However, as yet, the usefulness of 16S rDNA sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. In this study, we evaluated the utility of 16S rDNA sequencing as a means to identify a collection of 177 such isolates obtained from environmen
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Reischl, U., K. Feldmann, L. Naumann, et al. "16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene." Journal of Clinical Microbiology 36, no. 6 (1998): 1761–64. http://dx.doi.org/10.1128/jcm.36.6.1761-1764.1998.

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Direct sequencing of the 16S rRNA gene (16S rDNA) ofMycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.
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Grahn, Niclas, Mounira Hmani-Aifa, Karin Fransén, Peter Söderkvist, and Hans-Jürg Monstein. "Molecular identification of Helicobacter DNA present in human colorectal adenocarcinomas by 16S rDNA PCR amplification and pyrosequencing analysis." Journal of Medical Microbiology 54, no. 11 (2005): 1031–35. http://dx.doi.org/10.1099/jmm.0.46122-0.

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Seroepidemiological studies have indicated that Helicobacter pylori infection might be a possible risk factor for colorectal adenocarcinoma (CRC) development. However, limited information is available as to whether or not Helicobacter species are present in CRC tissues. In this study the presence of Helicobacter DNA in 77 CRC biopsies was investigated by means of a Helicobacter species-specific 16S rDNA PCR assay and real-time DNA pyrosequencing of the 16S rDNA variable V3 region. Pyrosequencing revealed the presence of Helicobacter DNA sequences in 21 of 77 biopsy specimens (27 %). 16S rDNA s
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BAO, Qiongli, Long-Jun DING, Yizong HUANG, and Keqing XIAO. "Effect of rice straw and/or nitrogen fertiliser inputs on methanogenic archaeal and denitrifying communities in a typical rice paddy soil." Earth and Environmental Science Transactions of the Royal Society of Edinburgh 109, no. 3-4 (2018): 375–86. http://dx.doi.org/10.1017/s1755691018000580.

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ABSTRACTTo understand better the microbial functional populations which are involved in methanogenesis and denitrification in paddy soils with rice straw (RS) and/or nitrogen fertiliser (potassium nitrate, N) application, the dynamics of methanogens and the denitrifying community were monitored simultaneously during the incubation period. The results show that the community structure of methanogens remained relatively stable among treatments based on 16S rDNA analysis, but fluctuated based on 16S rRNA. The Methanocellaceae and Methanosarcinaceae dominated all treatments at 16S rDNA and 16S rRN
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Dissertations / Theses on the topic "Morganii 16s rdna analysis"

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Ziegler, Katie. "Phylogenetic Analysis of a Group of Enteric Bacteria Based on 16S rDNA Gene Sequencing." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111684418.

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Wood, Jacqueline. "Analysis of bacterial populations from the rumen by means of 16S rDNA directed oligonucleotides." Thesis, University of Aberdeen, 1997. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU100186.

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The overall aim of this work was to develop molecular based techniques that would allow the identification and quantification of Prevotella spp. in samples of rumen fluid. This was achieved by developing two methods, both based on PCR amplification of 16S rDNA extracted from rumen and faecal samples. The first method involved the amplification of microbial DNA with universal primers, followed by probing with either a Bacteroides Prevotella specific oligonucleotide (Bac/Pre) or a universal oligonucleotide. Comparison with control DNA extracted from pure cultures allowed the relative abundances
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Al, Masalma Mouhamad. "Molecular and cultural analysis of the bacterial flora associated with brain abscesses." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX20663/document.

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Les abcès cérébraux sont des infections potentiellement mortelles, entraînant souvent des séquelles graves. La prise en charge médicale en reste empirique en raison d’un manque de connaissance approfondie des microorganismes responsables de cette condition. Dans la plupart des laboratoires microbiologiques, le diagnostic d’abcès cérébral est basé sur la culture du pus recueilli chirurgicalement. Malheureusement, cette procédure a de nombreuses limites et ne permet l’identification que d’une petite partie de la population microbienne en cause. L’amplification par PCR et le séquençage du gène co
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Fishman, Ross H. "The Molecular Analysis of the Biofilm of Proximal Incipient Caries in Young Permanent Teeth." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1244847351.

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Oberreuter, Helene. "FT-IR spectroscopic identification and infraspecific diversity of coryneform bacteria in relation to 16S rDNA sequence analysis." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962146374.

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Mills, John. "Bacterial Community Analysis of Meat Industry Conveyor Belts." The University of Waikato, 2007. http://hdl.handle.net/10289/2236.

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At the commencement of this study, some sensitive overseas markets were rejecting chilled vacuum-packed New Zealand lamb due to higher than expected total viable counts, and counts of Enterobacteriaceae, a family of bacteria used to indicate sanitary condition. Of the many factors that influence the bacterial composition of chilled lamb in the overseas marketplace, the meat producer can only exert significant control over: Hygiene, ensuring the bacterial viable count on the meat prior to packaging is as low as possible, and comprised of as few species as possible that are capable of anaerobic
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MUNDELL, J. NICOLE. "PHYLOGENETIC ANALYSIS OF KENTUCKY STRAINS OF XYLELLA FASTIDIOSA." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_theses/406.

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Phytopathogenic bacterium, Xylella fastidiosa, causes a number of economically important diseases, including Pierces disease (PD) of grape and bacterial leaf scorch (BLS) of a number of landscape trees. In Kentucky (KY), BLS affects a number of shade trees including many oak and maple species. In 2001, PD was diagnosed in grapevines in western KY. Xylella fastidiosa is also detected in many asymptomatic landscape plants and grasses. It was the goal of this research to identify hosts of X. fastidiosa around KY and use phylogenetic analysis to compare sequences of the 16S rDNA and gyrase B (gyrB
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Walsh, Kerry A. "Characterising the microbial community associated with a constructed wetland treating landfill leachate at Pitsea landfill site using 16S rDNA analysis." Thesis, University of East London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274628.

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Safina, Melania. "DNA barcoding of Pleuronectiformes: in silico analysis and development of markers." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/12259/.

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DNA barcoding is a method used for the identification and discovery of animal species. It usually involved a 648 base pair fragment of the mitochondrial cytochrome c oxidase subunit I, known as COI. This work is focused on the study of the genetic identification in the families belonging to the order Pleuronectiformes, commonly known as flatfish, and the accuracy of the genetic marker most used for the study of their DNA barcodes. The results indicate possible existence of taxonomical mistakes because several families do not show a gap between maximum intraspecific distance - which is the maxi
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Roth, McKenzie L. "Analysis of Bacterial Abundance and Species Diversity in Various Soils." Ashland University Honors Theses / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=auhonors1355166102.

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Book chapters on the topic "Morganii 16s rdna analysis"

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Rainey, Frederick A., and Erko Stackebrandt. "rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification." In Nonradioactive Analysis of Biomolecules. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57206-7_34.

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Sklarz, Menachem Y., Roey Angel, Osnat Gillor, and Ines M. Soares. "Amplified rDNA Restriction Analysis (ARDRA) for Identification and Phylogenetic Placement of 16S-rDNA Clones." In Handbook of Molecular Microbial Ecology I. John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118010518.ch7.

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Vinuesa, Pablo, L. W. Jan Rademaker, Frans J. de Bruijn, and Dietrich Werner. "Characterization of Bradyrhizobium SPP. Strains by Rflp analysis of Amplified 16s rDNA and rDNA Intergenic Spacer Regions." In Highlights of Nitrogen Fixation Research. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4795-2_56.

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Johansson, Karl-Erik, Malin U. K. Heldtander, and Bertil Pettersson. "Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers." In Mycoplasma Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-525-5:145.

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Dabert, M., J. Dabert, and K. Siuda. "Species validity of the soft-tick Argas polonicus (Acari: Argasidae) based on 16S rDNA sequence analysis." In Ecology and Evolution of the Acari. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-017-1343-6_58.

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Muyzer, Gerard, and Ellen C. de Waal. "Determination of the genetic diversity of microbial communities using DGGE analysis of PCR-amplified 16S rDNA." In Microbial Mats. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78991-5_21.

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Watanabe, Keiko, Norio Nagao, Tatsuki Toda, and Norio Kurosawa. "Bacterial Communities in Various Conditions of the Composting Reactor Revealed by 16S rDNA Clone Analysis and DGGE." In Sustainable Biotechnology. Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3295-9_8.

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Feye, Kristina M., and Steven C. Ricke. "Establishment of a Standardized 16S rDNA Library Preparation to Enable Analysis of Microbiome in Poultry Processing Using Illumina MiSeq Platform." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-9000-9_18.

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Vinuesa-Fleischmann, P., J. L. Rademaker, S. G. Pueppke, F. J. de Bruijn, and D. Wemer. "Characterization of Rhizobia Nodulating Endemic Woody Legumes of the Canary Islands by Computer-Assisted Analysis of Combined 16S rDNA-RFLP’s and rep-PCR Genomic Fingerprints." In Biological Nitrogen Fixation for the 21st Century. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_433.

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Jarabo-Lorenzo, A., E. Velázquez, R. Pérez-Galdona, et al. "Diversity of Bradyrhizobium Strains Isolated from Endemic Woody Legumes of the Canary Islands by PCR-Restriction Fragment Length Polymorphism Analysis of 16S rDNA and Low-Molecular-Weight Rna Profiles." In Nitrogen Fixation: From Molecules to Crop Productivity. Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-47615-0_102.

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Conference papers on the topic "Morganii 16s rdna analysis"

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Horn, Joanne, Celena Carrillo, and Victoria Dias. "Comparison of the Microbial Community Composition at Yucca Mountain and Laboratory Test Nuclear Repository Environments." In CORROSION 2003. NACE International, 2003. https://doi.org/10.5006/c2003-03556.

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Abstract The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain (YM), NV was determined. Microbial growth from collected rock was detected using simulated ground water as a growth medium, with or without amendment of a carbon source. Grown isolates were identified by 16S ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis of the microbial community located at the proposed nuclear waste repository site was performed using environmental DNA isolation and subsequent identification of amplified 16S rDNA genes. Concurrently, a
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Wang, Linna, Claudia C. Pierce, Dorothy Reynolds, and Elizabeth Summer. "DNA Based Diversity Analysis of Microorganisms in Industrial Cooling Towers." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09483.

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Abstract Effective microbial control in cooling systems is necessary to ensure system cleanliness and avoid fouling that degrades cooling system performance, promotes corrosion and favors growth of pathogens. However, controlling organisms optimally involves an understanding of the identity of the population of microbes in a system due to the varying susceptibilities of organisms to biocides. This is a challenging task with standard culturing techniques which only allow for a small fraction of the total population to be cultured and identified. In this study, 16s rDNA was employed to maximize
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De Paula, Renato, Cruz St Peter, Ian Alex Richardson, et al. "DNA Sequencing of Oilfield Samples: Impact of Protocol Choices on the Microbiological Conclusions." In CORROSION 2018. NACE International, 2018. https://doi.org/10.5006/c2018-11662.

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Abstract In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and
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Bartling, Craig, Angela Minard-Smith, Kate H. Kucharzyk, Jennifer Busch-Harris, and Larry Mullins. "Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09445.

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Abstract Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil and gas pipelines, wells, and a variety of surface facilities. Understanding of MIC is greatly enhanced through DNA and protein sequencing technologies. This paper highlights the need to understand the methods used to generate the data, the data quality, and the limitations associated with data interpretation through a case study involving the metagenomics and proteomic analysis of pig envelope debris and seawater samples from various locations within a seawater injection system sus
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Romero, J. M., E. Velázquez, J. L. García Villalobos, M. Amaya, and S. Le Borgne. "Genetic Monitoring of Bacterial Populations in a Seawater Injection System. Identification of Biocide Resistant Bacteria and Study of Their Corrosive Effect." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05483.

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Abstract DNA was extracted from a water sample taken from an offshore seawater injection system. DNA was also extracted from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the Polymerase Chain Reaction (PCR) and bacterial diversity was studied using Denaturing Gel Gradient Electrophoresis (DGGE). The obtained results showed that microbial evaluation was biased by the use of artificial culture media although recommended media were used, indicating that microbiological analysis of waters in industrial systems by culturing methods may n
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Norashirene, M. J., A. Ahmad Amin, D. Norhidayah, and M. A. Nurul Fithriah. "Identification of cellulolytic thermophiles based on 16S rDNA gene amplification analysis." In 2012 IEEE Colloquium on Humanities, Science and Engineering Research (CHUSER). IEEE, 2012. http://dx.doi.org/10.1109/chuser.2012.6504349.

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Xu, Xiaohong, Chundu Wu, and Degang Ning. "Detection Analysis of Pathogenic Organisms in Municipal Sewage with PCR-Based 16S rDNA Technique." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5518027.

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Li, He, Lin Li, Guoying Zhou, Yan Hao, and Yadi Liu. "On Optimization Study of Cultivation Conditions of a Flocculant-Producing Strain and 16s rDNA-Sequential Analysis." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163142.

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Rafedzi, E. A. K., M. Krsek, and E. M. H. Wellington. "The DGGE technique and 16S rDNA clone libraries analysis as a microbiological indicator of soil degradation." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0072.

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Liu Jun-ang, Pan Huaping, and Gou Zhihui. "Notice of Retraction: Screening and 16S rDNA sequence analysis of potassium bacterial strain from the Camellia oleifera rhizosphere environment." In 2010 2nd Conference on Environmental Science and Information Application Technology (ESIAT 2010). IEEE, 2010. http://dx.doi.org/10.1109/esiat.2010.5568886.

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Reports on the topic "Morganii 16s rdna analysis"

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Jurkevitch, Edouard, Carol Lauzon, Boaz Yuval, and Susan MacCombs. role of nitrogen-fixing bacteria in survival and reproductive success of Ceratitis capitata, the Mediterranean fruit fly. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7695863.bard.

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Objectives: to demonstrate nitrogen fixation in the gut of Ceratitiscapitata, the Mediterranean fruit fly and that fixed nitrogen is important for the fly. Background: Fruit flies (Diptera: Tephritidae) are a highly successful, widespread group of insects causing enormous economic damage in agriculture. They are anautogenous, i.e. the acquisition of nitrogenous compounds by both male and female is essential for the realization of their reproductive potential. Nitrogen, although abundant in the atmosphere, is paradoxically a limiting resource for multicellular organisms. In the Animalia, biolog
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