Relevant bibliographies by topics / MRC-5 human lung fibroblasts

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  2. Dissertations / Theses
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  4. Conference papers

Academic literature on the topic 'MRC-5 human lung fibroblasts'

Author: Grafiati
Published: 5 June 2025
Last updated: 2 August 2025

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Journal articles on the topic "MRC-5 human lung fibroblasts"

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Wang, Guo Hui, and Yong Peng Xie. "Effects of Mechanical Stimulation on Viscoelasticity of Human Lung Fibroblast." Applied Mechanics and Materials 432 (September 2013): 398–402. http://dx.doi.org/10.4028/www.scientific.net/amm.432.398.

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The mechanical stimulation can affect the biological function of lung cells, but little is known about the effects on biomechanical properties of lung fibroblasts. In this paper, the human lung fibroblasts (MRC-5) were subjected to cyclic stretch (5% and 10% elongation amplitude) by FX-4000 Tension System. The micropipette aspiration technique was used to investigate the viscoelasticity of MRC-5. The results: for 5% stretch group, the viscoelastic parameters of MRC-5 were significantly lower than static group at 72 h; for 10% stretch group, the viscoelastic parameters of MRC-5 were significant
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Yamamoto, R., L. S. Lin, R. Lowe, M. K. Warren, and T. J. White. "The human lung fibroblast cell line, MRC-5, produces multiple factors involved with megakaryocytopoiesis." Journal of Immunology 144, no. 5 (1990): 1808–16. http://dx.doi.org/10.4049/jimmunol.144.5.1808.

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Abstract The human lung fibroblast cell line MRC-5 constitutively produces a megakaryocyte potentiator activity, identified in murine bone marrow liquid culture assays for acetylcholinesterase and megakaryocyte colony assays in the presence of low concentrations of IL-3. The production levels of this activity were increased after stimulation with the phorbol ester analog, mezerein, and the calcium ionophore, A23187. Complete purification of a protein having this activity from conditioned media of induced MRC-5 cells was achieved using gel filtration and ion exchange chromatography. The first 1
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van Dijk, Eline M., Mark H. Menzen, Anita I. R. Spanjer, Laurens D. C. Middag, Corry-Anke A. Brandsma, and Reinoud Gosens. "Noncanonical WNT-5B signaling induces inflammatory responses in human lung fibroblasts." American Journal of Physiology-Lung Cellular and Molecular Physiology 310, no. 11 (2016): L1166—L1176. http://dx.doi.org/10.1152/ajplung.00226.2015.

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COPD is a progressive chronic lung disease characterized by pulmonary inflammation. Several recent studies indicate aberrant expression of WNT ligands and Frizzled receptors in the disease. For example, WNT-5A/B ligand expression was recently found to be increased in lung fibroblasts of COPD patients. However, possible effects of WNT-5A and WNT-5B on inflammation have not been investigated yet. In this study, we assessed the regulation of inflammatory cytokine release in response to WNT-5A/B signaling in human lung fibroblasts. Primary human fetal lung fibroblasts (MRC-5), and primary lung fib
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Kamalati, T., B. Thirunavukarasu, A. Wallace, et al. "Down-regulation of scatter factor in MRC 5 fibroblasts by epithelial-derived cells. A model for scatter factor modulation." Journal of Cell Science 101, no. 2 (1992): 323–32. http://dx.doi.org/10.1242/jcs.101.2.323.

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Scatter factor/hepatocyte growth factor (SF/HGF) is a multifunctional cytokine produced by embryonic fibroblasts and other mesenchymal cells that affects the growth and/or the movement of certain epithelia. Here we report that expression of scatter factor activity by MRC 5 cells, a strain of normal human embryonic lung fibroblasts, is greatly reduced as a result of co-culture of these cells with SVK14, an SV40-transformed human keratinocyte cell line. Using a cDNA probe to the beta chain of human HGF, we have found that the fall in SF activity in MRC 5/SVK14 co-cultures is accompanied by the l
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Vraneš, M., A. Tot, S. Jovanović-Šanta, et al. "Toxicity reduction of imidazolium-based ionic liquids by the oxygenation of the alkyl substituent." RSC Advances 6, no. 98 (2016): 96289–95. http://dx.doi.org/10.1039/c6ra16182k.

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Five different salicylate based ionic liquids were prepared in order to study their toxicity. Aquatic organisms (A. salina) and a human non-tumor cell line (normal fetal lung fibroblasts, MRC-5) were used for that purpose.
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Ljubičić, Jelena, Andrej Pešić, and Anđelka Isaković. "The effect of metformin on viability and mitochondrial status of tumor and non-tumor cell line." Medicinski podmladak 73, no. 4 (2022): 57–65. http://dx.doi.org/10.5937/mp73-37593.

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Introduction: Metformin is a drug of choice in the therapy of type II Diabetes mellitus. There is a growing evidence of metformin's antitumor activity, but the suggested mechanisms of such activity are still not fully elucidated. Aim: To investigate the effect of therapeutic doses of metformin on viability and mitochondrial status of human non-small cell lung carcinoma (NCI-H460) and human immortalized lung fibroblasts (MRC-5) cell lines. Material and methods: Acid phosphatase and Crystal Violet assays were used for the determination of NCI-H460 and MRC-5 cell viability after the treatment wit
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Zhao, Jihua, та Yao Yang. "Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways". Quality Assurance and Safety of Crops & Foods 14, № 1 (2022): 55–63. http://dx.doi.org/10.15586/qas.v14i1.1018.

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Persistent endoplasmic reticulum stress promotes aberrant inflammation and induces cell death, and inflammation is implicated in the pathogenesis of pneumonia. Vanillic acid exerts pharmacological activities, such as anti-inflammatory, antimicrobial, and antioxidant effects. However, the role of vanillic acid in pneumonia has not been elucidated yet. Human lung fibroblasts (WI-38 and MRC-5) were incubated with different concentrations of lipopolysaccharides to mimic the cell model of pneumonia. Lipopolysaccharides-treated lung fibroblasts were then incubated with different concentrations of va
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Yang, C. F., H. M. Shen, Y. Shen, Z. X. Zhuang, and C. N. Ong. "Cadmium-induced oxidative cellular damage in human fetal lung fibroblasts (MRC-5 cells)." Environmental Health Perspectives 105, no. 7 (1997): 712–16. http://dx.doi.org/10.1289/ehp.97105712.

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Li, Lingyu, Liyan Ma, Dongchun Wang та ін. "Design and Synthesis of Matrine Derivatives as Novel Anti-Pulmonary Fibrotic Agents via Repression of the TGFβ/Smad Pathway". Molecules 24, № 6 (2019): 1108. http://dx.doi.org/10.3390/molecules24061108.

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A total of 18 matrine derivatives were designed, synthesized, and evaluated for their inhibitory effect against TGF-β1-induced total collagen accumulation in human fetal lung fibroblast MRC-5 cell lines. Among them, compound 3f displayed the most potent anti-fibrotic activity (IC50 = 3.3 ± 0.3 μM) which was 266-fold more potent than matrine. 3f significantly inhibited the fibroblast-to-myofibroblast transition and extracellular matrix production of MRC-5 cells. The TGF-β/small mothers against decapentaplegic homologs (Smad) signaling was also inhibited by 3f, as evidenced by inhibition of cyto
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Yi, Minxiao, Bo Liu, Yang Tang, Fang Li, Wan Qin, and Xianglin Yuan. "Irradiated Human Umbilical Vein Endothelial Cells Undergo Endothelial-Mesenchymal Transition via the Snail/miR-199a-5p Axis to Promote the Differentiation of Fibroblasts into Myofibroblasts." BioMed Research International 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/4135806.

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Radiation induced pulmonary fibrosis (RIPF) is one of the major side effects of radiotherapy for lung cancer. Previous studies have shown that endothelial cells and activated myofibroblasts play a key role in RIPF. However, the interaction between irradiated endothelial cells and activation of myofibroblasts has not been reported. The aim of the present study was to examine whether irradiated endothelial cells would affect the differentiation of fibroblasts into myofibroblasts in the process of RIPF. In the current study, we used a coculture system that allowed direct contact between human fet
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Dissertations / Theses on the topic "MRC-5 human lung fibroblasts"

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Yates, Sally A. "The cytotoxic effects of malondialdehyde on human lung fibroblast cells." Thesis, Liverpool John Moores University, 2015. http://researchonline.ljmu.ac.uk/4529/.

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Malondialdehyde (MDA) is a mutagenic and carcinogenic product of lipid peroxidation which has also been found at elevated levels in smokers. MDA reacts with nucleic acid bases to form pyrimidopurinone DNA adducts, of which 3-(2-deoxy-β-D-erythro-pentofuranosyl)pyrimidol[1,2-α]purin-10(3H)-one (M1dG) is the most abundant and has been linked to smoking. Mutations in the TP53 tumour suppressor gene are associated with half of all cancers. This research applied a multidisciplinary approach to investigate the toxic effects of MDA on the human lung fibroblasts MRC5, which have an intact p53 response
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Lang, Yaw-Dong, and 藍耀東. "The Molecular Mechanism of Paraquat-induced Collagen Production in Human Lung Fibroblast MRC-5 Cells." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/84078775563718198216.

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碩士<br>臺北醫學大學<br>醫學研究所<br>94<br>Paraquat dichloride (1,1-dimethyl-4,4-bipyridilium dichloride; methyl viologen) is an effective and widely used herbicide. According to epidemiological evidence in the National Poison Center in Taiwan, paraquat poisoning has suggested that was the leading cause of poisoning-induced death in Taiwan. In this study, the normal human fetal lung fibroblast cell line (MRC-5) was used to study the mechanism of paraquat-induced pulmonary fibrosis for eliminating the effect of paraquat nephrotoxicity on renin-angiotensin system (RAS). The results showed that paraquat-indu
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Shao-Chun and 曾邵群. "Studying House dust mite allergen Der P 2 induced proliferation and collagen overexpression of human lung fibroblast MRC-5." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/89yfxu.

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碩士<br>中山醫學大學<br>生化暨生物科技研究所<br>100<br>Der p 2 (DP2), a Dermatophagoides pteronyssinus group 2 allergen, is well-known to cause airway hypersensitivity and highly associates with induction and progression of asthma. Previous studies have shown that DP2 activates several mitogen-activated protein kinase (MAPK) pathways, which is also known to mediate muscarinic receptor-induced human lung fibroblast proliferation. Airway fibroblast proliferation and the consequent fibrosis is an important pathological characteristic of chronic inflammatory and obstructive airway diseases such as asthma, COPD and
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Wu, Yi-Fang, and 吳懿芳. "Investigation of suppressive effects of rosmarinic acid on profibrotic gene expression of human lung fibroblast MRC-5 in response to house dust mite allergen Der p 2." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/18872475455646469066.

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碩士<br>中山醫學大學<br>生化暨生物科技研究所<br>102<br>House dust mites (HDMs) are the major cause leading to airway hypersensitivity in Taiwan and developed countries.In Taiwan, ther are three main species of HDMs leading to allergic disoders,includingDermatophagoides pteronyssinus, Dermatophagoides farina, and Blomia tropicalis. Among the HDMs, pathogenic mechanisms of Dermatophagoides pteronyssinus-induced allergies arewidely investigated and allergens derived from the mite are mostly identified and characterized. Previous studies have indicated that severe respiratory hypersensitiveness maycontribute to fur
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Jeng-Sheng, Wu, and 吳瑞昇. "Studies on the Pathway of Cell Death Triggered by Cadmium and Mercury in Human Normal Lung Cell MRC-5." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/97155841996348439824.

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碩士<br>台北醫學院<br>醫學研究所<br>90<br>Epidemiological evidence has suggested that exposure of the human to cadmium (Cd) and mercury (Hg) might cause pulmonary damages. However, the mechanism has remained unclear. A normal human fetal lung fibroblast cell line (MRC-5) was used as a cell model to investigate the mechanism of cell death triggered by Cd or Hg. Several methods were employed to elucidate apoptosis and necrosis of MRC-5 cells after treatment of 100μM Cd or Hg, respectively; including cytohistochemistry analysis with Hoechst staining, flow cytometric assay with propidium iodide (PI) single st
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Wang, Chia-Jen, and 王嘉媜. "Study on the Effects of ROS during Apoptosis Triggered by Cadmium and Mercury in Human Normal Lung Cell MRC-5." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/14507749521979863332.

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碩士<br>台北醫學院<br>醫學研究所<br>88<br>Epidemiological evidence suggested that cadmium (Cd) and mercury (Hg) exposure might cause pulmonary damage. However, the mechanisms were not clear yet. Cd or Hg had been reported to induce apoptosis in some cell lines and might correlate with redox signaling. In our research, a normal human fetal lung fibroblast cell line (MRC-5) was used as a cell model to investigate the effects of reactive oxygen species (ROS) during apoptosis triggered by Cd or Hg. Among numerous antioxidant compounds or enzymes, N- acetylcysteine (NAC)、pyrrolidine dithiocarbamate (PDTC)、asco
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Book chapters on the topic "MRC-5 human lung fibroblasts"

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Collins, Arlene R. "Induction of Apoptosis in MRC-5, Diploid Human Fetal Lung Cells after Infection with Human Coronavirus OC43." In Advances in Experimental Medicine and Biology. Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-1325-4_100.

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"Cytotoxic activity of macrocyclic Lu (III) complexes." In Book of Abstracts - RAD 2025 Conference. RAD Centre, Niš, Serbia, 2025. https://doi.org/10.21175/rad.abstr.book.2025.21.2.

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Aim: Two newly synthesized complexes [[Lu2tpmcCl2(H2O)2](BF4)4∙2NaBF4 and [Lucyc(H2O)2] (BF4)3∙NaBF4 were tested against human lung carcinoma cell line A549 and the non-malignant, human lung fibroblast cell line MRC-5. The values of IC50 were calculated. Material and Methods. Cell Culture. The human lung carcinoma cell line A549 (CCL-185) and the non-malignant human lung fibroblast cell line MRC-5 (CCL-171) were procured from the American Type Culture Collection. The cells were grown in Dulbecco's Modified Eagle Medium (DMEM), supplemented with 10% fetal bovine serum, and a solution of penicil
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Conference papers on the topic "MRC-5 human lung fibroblasts"

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Ganić, Tea, Dejan Pljevljakušić, U. Babović, et al. "CINNAMON ESSENTIAL OIL AS AN ANTIMICROBIAL AND ANTIBIOFILM AGENT AGAINST FOODBORNE PATHOGENS." In 8th Workshop Food and Drug Safety and Qualit. Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, 2024. http://dx.doi.org/10.46793/8fdsq.pd5tg.

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Foodborne pathogens present a serious human health problem, especially in the era of growing antimicrobial resistance. To overcome the problem, the solution was sought in plants, rich in secondary metabolites. Cinnamon essential oil (EO) is recognized as a natural product with antibacterial potential. The aim of the study was to chemically characterize EO, to determine antibacterial and antibiofilm potential against foodborne pathogens, and to estimate cytotoxicity. HPLC pointed out eugenol as the most abundant component, while microdilution assay showed moderate antibacterial activity (the hi
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Milenković, Dejan A., Marko N. Živanović, Milan S. Dekić, Marijana Stanojević Pirković, and Jelena R. Đorović Jovanović. "CYTOTOXIC ACTIVITY AND MOLECULAR DOCKING STUDY OF 4- SUBSTITUTED FLAVYLIUM SALT." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac,, 2021. http://dx.doi.org/10.46793/iccbi21.466m.

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In the present manuscript, the cytotoxic activity of flavylium cation substituted at 4- position with phenyl (FC-4Ph) was tested to two cells lines (human colorectal carcinoma, HCT-116, and human fibroblast lung, MRC-5). In vitro cytotoxicity experiments were performed to elucidate the possible anticancer activity of tested substance. Investigated compound did not show cytotoxic effect on HCT-116 after 24 h, while after 72 h exerted significant effect. A significant selectivity towards colorectal carcinoma cells was observed. On the other hand, this compound did not show any effect on MRC-5 ce
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Ward, Mark, David E. Smart, James Roberts, et al. "The epigenetic modulator 5-aza-2’-deoxycytidine reduces the fibrotic response of human lung fibroblasts to TGFß1 in a novel, long term, 3D cell culture system." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa2430.

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Wernet, P., M. Haurand, W. Nüsing, E. M. Schneider, K. Jaschonek, and V. Ullrich. "Production and characterization of a murine monoclonal antibody against human thromboxane synthetase." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643382.

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Eicosanoids appear to have an important role in the actual momentary regulation of tissue blood flow. The function of constricting blood vessels by affecting the vascular tone has been assigned to thromboxane. Thromboxane synthetase, the enzyme responsible for the conversion of Prostaglandin-H2 into thromboxane A2, has been shown to be present in platelets, lung fibroblasts and the brain. Recently, thromboxane synthetase has been totally purified. The enzyme isolated from platelets appears to have a molecular weight of 58,800 Dalton and to belong to the group of cytochrome P450 proteins. In or
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Larionov, A., T. V. Leshukov, and O. S. Yakovenko. "BIOLOGICAL EFFECTS AND FRACTIONAL COMPOSITION OF COAL-ROCK PARTICLE MATTER." In I International Congress “The Latest Achievements of Medicine, Healthcare, and Health-Saving Technologies”. Kemerovo State University, 2023. http://dx.doi.org/10.21603/-i-ic-71.

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Aim: to evaluate separately the fractional composition of dust particles (PM – particle matter)&#x0D; micro- (from 10 μm to 100 nm, PM10) and nanofractions (less than 100 nm, PM0.1), as well as their&#x0D; biological effect by the DNA comet method on a culture of lung fibroblasts MRC-5. The most&#x0D; abundant were 1.1-1.3 µm and 50-80 nm particles in micro- and nanofractions, respectively. A high&#x0D; level of DNA disease is found in samples exposed to PM0.1 in searches with PM10.
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Ignjatijević, Ana, Stefana Vuletić, Biljana Nikolić, et al. "EVALUATION OF ANTIGENOTOXIC POTENTIAL OF METHANOLIC AND DICHLOROMETHANE EXTRACTS OF Achillea millefolium." In 8th Workshop Food and Drug Safety and Qualit. Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, 2024. http://dx.doi.org/10.46793/8fdsq.pc3ai.

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Genotoxicity refers to the ability of various agents, called genotoxins, to induce damage to genetic material. Given their involvement in various human diseases, exploring new protective compounds from natural sources, especially plants, is of crucial importance today. With that in mind, our study evaluated the potential antigenotoxic effect of methanolic and dichloromethane extracts of traditionally used plant Achillea millefolium on normal fetal fibroblasts (MRC-5). The screening of cytotoxicity was conducted using an MTT assay to determine non-cytotoxic concentrations of extracts on MRC-5 c
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Soldatovic, Tanja, Asija Halilagic, Enisa Selimovic, et al. "Viability and oxidative response of human colorectal HCT-116 cancer and human lung healthy pleura MRC-5 cell lines treated with novel bridged heteronuclear Zn(II)-L-Cu(II)." In 6th International Electronic Conference on Medicinal Chemistry. MDPI, 2020. http://dx.doi.org/10.3390/ecmc2020-07447.

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Đorđević, N., O. Keta, V. Petković, et al. "„In vitro“ biological effects of clonal red wines." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.180dj.

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This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT
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Andreasen, P. A., A. Riccio, L. R. Lund, K. G. Welinder, F. Blasi, and K. Danø. "PLASMINOGEN ACTIVATOR INHIBITOR TYPE 1: STUDIES ON STRUCTURE AND REGULATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642810.

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Human plasminogen activator inhibitor type-1 is an Mr∼54,000 protein which specifically inhibits urokinase-type (u-PA) and tissue-type (t-PA) plasminogen activators. During inhibition, u-PA and t-PA convert PAI-1 to an inactive form with Mr∼50,000. We have determined the amino-terminal amino acid sequence of native and converted PAI-1, and isolated and partly sequenced PAI-1 cDNA. The data show that the conversion of PAI-1 consists of cleavage of an Arg-Met bond 33 residues from the carboxy-terminus, thus localizing the reactive center of the inhibitor to that position, and identifying PAI-1 a
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