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Journal articles on the topic 'MRC-5 human lung fibroblasts'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Wang, Guo Hui, and Yong Peng Xie. "Effects of Mechanical Stimulation on Viscoelasticity of Human Lung Fibroblast." Applied Mechanics and Materials 432 (September 2013): 398–402. http://dx.doi.org/10.4028/www.scientific.net/amm.432.398.

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The mechanical stimulation can affect the biological function of lung cells, but little is known about the effects on biomechanical properties of lung fibroblasts. In this paper, the human lung fibroblasts (MRC-5) were subjected to cyclic stretch (5% and 10% elongation amplitude) by FX-4000 Tension System. The micropipette aspiration technique was used to investigate the viscoelasticity of MRC-5. The results: for 5% stretch group, the viscoelastic parameters of MRC-5 were significantly lower than static group at 72 h; for 10% stretch group, the viscoelastic parameters of MRC-5 were significant

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2

Yamamoto, R., L. S. Lin, R. Lowe, M. K. Warren, and T. J. White. "The human lung fibroblast cell line, MRC-5, produces multiple factors involved with megakaryocytopoiesis." Journal of Immunology 144, no. 5 (1990): 1808–16. http://dx.doi.org/10.4049/jimmunol.144.5.1808.

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Abstract The human lung fibroblast cell line MRC-5 constitutively produces a megakaryocyte potentiator activity, identified in murine bone marrow liquid culture assays for acetylcholinesterase and megakaryocyte colony assays in the presence of low concentrations of IL-3. The production levels of this activity were increased after stimulation with the phorbol ester analog, mezerein, and the calcium ionophore, A23187. Complete purification of a protein having this activity from conditioned media of induced MRC-5 cells was achieved using gel filtration and ion exchange chromatography. The first 1

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3

van Dijk, Eline M., Mark H. Menzen, Anita I. R. Spanjer, Laurens D. C. Middag, Corry-Anke A. Brandsma, and Reinoud Gosens. "Noncanonical WNT-5B signaling induces inflammatory responses in human lung fibroblasts." American Journal of Physiology-Lung Cellular and Molecular Physiology 310, no. 11 (2016): L1166—L1176. http://dx.doi.org/10.1152/ajplung.00226.2015.

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COPD is a progressive chronic lung disease characterized by pulmonary inflammation. Several recent studies indicate aberrant expression of WNT ligands and Frizzled receptors in the disease. For example, WNT-5A/B ligand expression was recently found to be increased in lung fibroblasts of COPD patients. However, possible effects of WNT-5A and WNT-5B on inflammation have not been investigated yet. In this study, we assessed the regulation of inflammatory cytokine release in response to WNT-5A/B signaling in human lung fibroblasts. Primary human fetal lung fibroblasts (MRC-5), and primary lung fib

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4

Kamalati, T., B. Thirunavukarasu, A. Wallace, et al. "Down-regulation of scatter factor in MRC 5 fibroblasts by epithelial-derived cells. A model for scatter factor modulation." Journal of Cell Science 101, no. 2 (1992): 323–32. http://dx.doi.org/10.1242/jcs.101.2.323.

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Scatter factor/hepatocyte growth factor (SF/HGF) is a multifunctional cytokine produced by embryonic fibroblasts and other mesenchymal cells that affects the growth and/or the movement of certain epithelia. Here we report that expression of scatter factor activity by MRC 5 cells, a strain of normal human embryonic lung fibroblasts, is greatly reduced as a result of co-culture of these cells with SVK14, an SV40-transformed human keratinocyte cell line. Using a cDNA probe to the beta chain of human HGF, we have found that the fall in SF activity in MRC 5/SVK14 co-cultures is accompanied by the l

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Vraneš, M., A. Tot, S. Jovanović-Šanta, et al. "Toxicity reduction of imidazolium-based ionic liquids by the oxygenation of the alkyl substituent." RSC Advances 6, no. 98 (2016): 96289–95. http://dx.doi.org/10.1039/c6ra16182k.

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Five different salicylate based ionic liquids were prepared in order to study their toxicity. Aquatic organisms (A. salina) and a human non-tumor cell line (normal fetal lung fibroblasts, MRC-5) were used for that purpose.

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Ljubičić, Jelena, Andrej Pešić, and Anđelka Isaković. "The effect of metformin on viability and mitochondrial status of tumor and non-tumor cell line." Medicinski podmladak 73, no. 4 (2022): 57–65. http://dx.doi.org/10.5937/mp73-37593.

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Introduction: Metformin is a drug of choice in the therapy of type II Diabetes mellitus. There is a growing evidence of metformin's antitumor activity, but the suggested mechanisms of such activity are still not fully elucidated. Aim: To investigate the effect of therapeutic doses of metformin on viability and mitochondrial status of human non-small cell lung carcinoma (NCI-H460) and human immortalized lung fibroblasts (MRC-5) cell lines. Material and methods: Acid phosphatase and Crystal Violet assays were used for the determination of NCI-H460 and MRC-5 cell viability after the treatment wit

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Zhao, Jihua, та Yao Yang. "Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways". Quality Assurance and Safety of Crops & Foods 14, № 1 (2022): 55–63. http://dx.doi.org/10.15586/qas.v14i1.1018.

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Persistent endoplasmic reticulum stress promotes aberrant inflammation and induces cell death, and inflammation is implicated in the pathogenesis of pneumonia. Vanillic acid exerts pharmacological activities, such as anti-inflammatory, antimicrobial, and antioxidant effects. However, the role of vanillic acid in pneumonia has not been elucidated yet. Human lung fibroblasts (WI-38 and MRC-5) were incubated with different concentrations of lipopolysaccharides to mimic the cell model of pneumonia. Lipopolysaccharides-treated lung fibroblasts were then incubated with different concentrations of va

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Yang, C. F., H. M. Shen, Y. Shen, Z. X. Zhuang, and C. N. Ong. "Cadmium-induced oxidative cellular damage in human fetal lung fibroblasts (MRC-5 cells)." Environmental Health Perspectives 105, no. 7 (1997): 712–16. http://dx.doi.org/10.1289/ehp.97105712.

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9

Li, Lingyu, Liyan Ma, Dongchun Wang та ін. "Design and Synthesis of Matrine Derivatives as Novel Anti-Pulmonary Fibrotic Agents via Repression of the TGFβ/Smad Pathway". Molecules 24, № 6 (2019): 1108. http://dx.doi.org/10.3390/molecules24061108.

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A total of 18 matrine derivatives were designed, synthesized, and evaluated for their inhibitory effect against TGF-β1-induced total collagen accumulation in human fetal lung fibroblast MRC-5 cell lines. Among them, compound 3f displayed the most potent anti-fibrotic activity (IC50 = 3.3 ± 0.3 μM) which was 266-fold more potent than matrine. 3f significantly inhibited the fibroblast-to-myofibroblast transition and extracellular matrix production of MRC-5 cells. The TGF-β/small mothers against decapentaplegic homologs (Smad) signaling was also inhibited by 3f, as evidenced by inhibition of cyto

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Yi, Minxiao, Bo Liu, Yang Tang, Fang Li, Wan Qin, and Xianglin Yuan. "Irradiated Human Umbilical Vein Endothelial Cells Undergo Endothelial-Mesenchymal Transition via the Snail/miR-199a-5p Axis to Promote the Differentiation of Fibroblasts into Myofibroblasts." BioMed Research International 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/4135806.

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Radiation induced pulmonary fibrosis (RIPF) is one of the major side effects of radiotherapy for lung cancer. Previous studies have shown that endothelial cells and activated myofibroblasts play a key role in RIPF. However, the interaction between irradiated endothelial cells and activation of myofibroblasts has not been reported. The aim of the present study was to examine whether irradiated endothelial cells would affect the differentiation of fibroblasts into myofibroblasts in the process of RIPF. In the current study, we used a coculture system that allowed direct contact between human fet

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Bażanów, Barbara, Katarzyna Michalczyk, Alina Kafel, et al. "The Effects of Different Respiratory Viruses on the Oxidative Stress Marker Levels in an In Vitro Model: A Pilot Study." International Journal of Molecular Sciences 25, no. 22 (2024): 12088. http://dx.doi.org/10.3390/ijms252212088.

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Respiratory viruses are among the most common causes of human infections. Examining pathological processes linked to respiratory viral infections is essential for diagnosis, treatment strategies, and developing novel therapeutics. Alterations in oxidative stress levels and homeostasis are significant processes associated with respiratory viral infections. The study aimed to compare selected oxidative stress markers: total oxidative status (TOS), total antioxidant capacity (TAC), and the oxidative stress index (OSI) levels and glutathione peroxidase (GPx) and glutathione reductase (GR) activiti

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Jojic, Nikola, Vesna Kojic, Danijela Kojic, Karmen Stankov, and Gordana Bogdanovic. "Cytotoxicity of single-walled carbon nanotubes to human lung carcinoma cells: The influence of N-acetylcysteine." Archive of Oncology 21, no. 2 (2013): 59–61. http://dx.doi.org/10.2298/aoo1302059j.

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Background: Single-walled carbon nanotubes (SWCNTs) have been reported to induce cytotoxicity in different cell lines. Although the mechanisms underlying cytotoxicity are not fully understood, accumulation of reactive oxygen species (ROS) and oxidative damage is considered to be a likely contributing factor. Methods: Human lung carcinoma cells, A549, and human fetal lung fibroblasts, MRC-5 were used to assess the cytotoxicity of SWCNT in the presence and absence of a redox status regulator, N-acetylcysteine (NAC), via the MTT assay. Results: SWCNT induced a nearly three-fold greater loss of vi

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Marthandan, Shiva, Steffen Priebe, Mario Baumgart, et al. "Similarities in Gene Expression Profiles duringIn VitroAging of Primary Human Embryonic Lung and Foreskin Fibroblasts." BioMed Research International 2015 (2015): 1–17. http://dx.doi.org/10.1155/2015/731938.

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Replicative senescence is of fundamental importance for the process of cellular aging, since it is a property of most of our somatic cells. Here, we elucidated this process by comparing gene expression changes, measured by RNA-seq, in fibroblasts originating from two different tissues, embryonic lung (MRC-5) and foreskin (HFF), at five different time points during their transition into senescence. Although the expression patterns of both fibroblast cell lines can be clearly distinguished, the similar differential expression of an ensemble of genes was found to correlate well with their transit

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Liu, Sen, Yan Wang, Huijuan Wen, Xiaofang Sun та Yu Wang. "Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway". Evidence-Based Complementary and Alternative Medicine 2019 (26 грудня 2019): 1–9. http://dx.doi.org/10.1155/2019/4050327.

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Objective. Hydroxysafflor yellow A (HSYA), an effective ingredient of the Chinese herb Carthamus tinctorius L, attenuated bleomycin-induced pulmonary fibrosis in mice. This study is to investigate the effect of HSYA on the proliferation and inflammatory level of human fetal lung fibroblasts (MRC-5 cells) induced by tumor necrosis factor-α (TNF-α) and explore the underlying mechanisms. Methods. MRC-5 cells were treated with different concentrations of TNF-α, HSYA, or/and etanercept (ENCP, TNF-α receptor (TNFR1) antagonist, 500 ng/mL) before cell proliferation was detected. The laser confocal mi

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Voicu, Sorina, Mihaela Balas, Miruna Stan, et al. "Amorphous Silica Nanoparticles Obtained by Laser Ablation Induce Inflammatory Response in Human Lung Fibroblasts." Materials 12, no. 7 (2019): 1026. http://dx.doi.org/10.3390/ma12071026.

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Silica nanoparticles (SiO2 NPs) represent environmentally born nanomaterials that are used in multiple biomedical applications. Our aim was to study the amorphous SiO2 NP-induced inflammatory response in MRC-5 human lung fibroblasts up to 72 hours of exposure. The intracellular distribution of SiO2 NPs was measured by transmission electron microscopy (TEM). The lactate dehydrogenase (LDH) test was used for cellular viability evaluation. We have also investigated the lysosomes formation, protein expression of interleukins (IL-1β, IL-2, IL-6, IL-8, and IL-18), COX-2, Nrf2, TNF-α, and nitric oxid

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Zhang, N., Y.-P. Xie, L. Pang, et al. "Paraquat increases connective tissue growth factor expression and impairs lung fibroblast proliferation and viscoelasticity." Human & Experimental Toxicology 33, no. 12 (2014): 1232–40. http://dx.doi.org/10.1177/0960327114524239.

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This in vitro study was designed to investigate the molecular mechanisms of paraquat-induced damage using cultured human fetal lung fibroblasts (MRC-5 cells), in order to promote the development of improved therapies for paraquat poisoning. Paraquat’s effects on proliferation were examined by flow cytometry, on viscoelasticity by the micropipette aspiration technique, and on connective tissue growth factor (CTGF) expression by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Paraquat was found to significantly reduce the proliferation index of MRC-5 cells in a concent

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17

de Godoy, Maria Carolina Ximenes, Juliana Alves Macedo, and Alessandra Gambero. "Researching New Drug Combinations with Senolytic Activity Using Senescent Human Lung Fibroblasts MRC-5 Cell Line." Pharmaceuticals 17, no. 1 (2024): 70. http://dx.doi.org/10.3390/ph17010070.

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Therapeutically targeting senescent cells seems to be an interesting perspective in treating chronic lung diseases, which are often associated with human aging. The combination of the drug dasatinib and the polyphenol quercetin is used in clinical trials as a senolytic, and the first results point to the relief of physical dysfunction in patients with idiopathic pulmonary fibrosis. In this work, we tested new combinations of drugs and polyphenols, looking for senolytic activity using human lung fibroblasts (MRC-5 cell line) with induced senescence. We researched drugs, such as azithromycin, ra

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18

Yonei, Sayuri, Koji Tamura, Eiki Koyama, Tsutomu Nohno, Sumihare Noji, and Hiroyuki Ide. "MRC-5, Human Embryonic Lung Fibroblasts, Induce the Duplication of the Developing Chick Limb Bud." Developmental Biology 160, no. 1 (1993): 246–53. http://dx.doi.org/10.1006/dbio.1993.1302.

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19

Godoy, Maria Carolina Ximenes de, Gabriela Arruda Monteiro, Bárbara Hakim de Moraes, Juliana Alves Macedo, Gisele Mara Silva Gonçalves, and Alessandra Gambero. "Addition of Polyphenols to Drugs: The Potential of Controlling “Inflammaging” and Fibrosis in Human Senescent Lung Fibroblasts In Vitro." International Journal of Molecular Sciences 25, no. 13 (2024): 7163. http://dx.doi.org/10.3390/ijms25137163.

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The combination of a polyphenol, quercetin, with dasatinib initiated clinical trials to evaluate the safety and efficacy of senolytics in idiopathic pulmonary fibrosis, a lung disease associated with the presence of senescent cells. Another approach to senotherapeutics consists of controlling inflammation related to cellular senescence or “inflammaging”, which participates, among other processes, in establishing pulmonary fibrosis. We evaluate whether polyphenols such as caffeic acid, chlorogenic acid, epicatechin, gallic acid, quercetin, or resveratrol combined with different senotherapeutics

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20

Son, ES, SY Kyung, SP Lee та ін. "Role of protein kinase C-η in cigarette smoke extract-induced apoptosis in MRC-5-cells". Human & Experimental Toxicology 34, № 9 (2014): 869–77. http://dx.doi.org/10.1177/0960327114561343.

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Cigarette smoke (CS) is a major risk factor for emphysema, which causes cell death in structural cells of the lung by mechanisms that are still not completely understood. We demonstrated previously that CS extract (CSE) induces caspase activation in MRC-5 human lung fibroblasts, activated protein kinase C-η (PKC-η), and translocated PKC-η from the cytosol to the membrane. The objective of this study was to investigate the involvement of PKC-η activation in a CSE-induced extrinsic apoptotic pathway. We determined that CSE increases expression of caspase 3 and 8 cleavage in MRC-5 cells and overe

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Takii, Takemasa, Yoshifumi Yamamoto, Taku Chiba, et al. "Simple Fibroblast-Based Assay for Screening of New Antimicrobial Drugs against Mycobacterium tuberculosis." Antimicrobial Agents and Chemotherapy 46, no. 8 (2002): 2533–39. http://dx.doi.org/10.1128/aac.46.8.2533-2539.2002.

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ABSTRACT In this study, we propose a simple and reproducible host-cell-based assay for the screening of antimycobacterial drugs that is suitable for drug discovery. The method evaluates both antimycobacterial activity of the drugs and their cytotoxicity to host cells. The basis of this simple fibroblast-based assay (SFA) is that cells of human lung fibroblast cell line MRC-5, which are highly sensitive to mycobacterial cytotoxicity, are killed by virulent Mycobacterium tuberculosis strain H37Rv bacilli in response to the viability of bacilli. Clinically used antimycobacterial drugs inhibited t

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Niimi, Shingo, Masashi Hyuga, Hiromi Kazama, et al. "Activins A, AB, and B Inhibit Hepatocyte Growth Factor Synthesis by MRC-5 Human Lung Fibroblasts." Biological & Pharmaceutical Bulletin 25, no. 11 (2002): 1405–8. http://dx.doi.org/10.1248/bpb.25.1405.

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Huang, Shao-Min, Xinbing Zuo, Jasmine Jia'En Li, Sam Fong Yau Li, Boon Huat Bay, and Choon Nam Ong. "Metabolomics Studies Show Dose-Dependent Toxicity Induced by SiO2Nanoparticles in MRC-5 Human Fetal Lung Fibroblasts." Advanced Healthcare Materials 1, no. 6 (2012): 779–84. http://dx.doi.org/10.1002/adhm.201200114.

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Fehr, Anthony R., and Dong Yu. "Human Cytomegalovirus Gene UL21a Encodes a Short-Lived Cytoplasmic Protein and Facilitates Virus Replication in Fibroblasts." Journal of Virology 84, no. 1 (2009): 291–302. http://dx.doi.org/10.1128/jvi.01116-09.

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ABSTRACT The human cytomegalovirus (HCMV) gene UL21a was recently annotated by its conservation in chimpanzee cytomegalovirus. Two large-scale mutagenic analyses showed that mutations in overlapping UL21a/UL21 resulted in a severe defect of virus growth in fibroblasts. Here, we characterized UL21a and demonstrated its role in HCMV infection. We mapped a UL21a-specific transcript of ∼600 bp that was expressed with early kinetics. UL21a encoded pUL21a, a protein of ∼15 kDa, which was unstable and localized predominantly to the cytoplasm during HCMV infection or when expressed alone. Interestingl

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Koh, Eun-Jeong, Seong-Yeong Heo, Areumi Park, Yeon-Ji Lee, Woon-Yong Choi, and Soo-Jin Heo. "Serum-Free Medium Supplemented with Haematococcus pluvialis Extracts for the Growth of Human MRC-5 Fibroblasts." Foods 13, no. 18 (2024): 3012. http://dx.doi.org/10.3390/foods13183012.

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Experiments are increasingly performed in vitro; therefore, cell culture technology is essential for scientific progress. Fetal bovine serum (FBS) is a key cell culture supplement providing growth factors, amino acids, and hormones. However, FBS is not readily available on the market, has contamination risks, and has ethical concerns. This study aimed to investigate Haematococcus pluvialis extracts (HE) as a potential substitute for FBS. Therefore, we assessed the effects of HE on cell maintenance, growth, and cycle progression in human lung fibroblasts (MRC-5). Cell progression and monosaccha

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Farkas, Sándor, Goran Benedekovic, Sladjana Stanisavljevic, et al. "Synthesis and antiproliferative activity of (5R)-cleistenolide and analogues." Journal of the Serbian Chemical Society, no. 00 (2023): 18. http://dx.doi.org/10.2298/jsc230126018f.

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(5R)-Cleistenolide and a few related analogues have been synthesized starting from d-glucose. The key steps of the synthesis included a Z-selective Wittig olefination and an intramolecular Mitsunobu reaction with an inversion of configuration at the C-5 position. In vitro antiproliferative activity of synthesized compounds was tested on a panel of eight human tumour cells and against a single normal cell line (MRC-5). The majority of tested compounds showed strong antiproliferative effects on certain human tumour cells and all of them showed negligible toxicity to normal foetal lung fibroblast

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Chang, Hui-Chiu, Wen-Chun Hung, Ming-Shyan Huang, and Hseng-Kuang Hsu. "Extract from the Leaves of Toona sinensis Roemor Exerts Potent Antiproliferative Effect on Human Lung Cancer Cells." American Journal of Chinese Medicine 30, no. 02n03 (2002): 307–14. http://dx.doi.org/10.1142/s0192415x02000223.

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Recent study indicated that the components of Toona sinensis Roemor have potent anti-inflammatory and analgesic effects. These components have also been reported to inhibit the growth of boils in vivo. In this study, we investigated the effect of crude extract from the leaves of Toona sinensis Roemor on the proliferation of A549 lung cancer cells. We found that the extract effectively blocked cell cycle progression by inhibiting the expression of cyclin D1 and E in A549 cells. Additionally, incubation of the extract led to activation of caspase-3-like proteases and apoptotic cell death. Conver

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Kang, Shin Myung, Jin Young Yoon, Yu Jin Kim, Sang Pyo Lee, Sung Hwan Jeong, and Jeong-Woong Park. "Inhibition of PKC Epsilon Attenuates Cigarette Smoke Extract-Induced Apoptosis in Human Lung Fibroblasts (MRC-5 Cells)." Tuberculosis and Respiratory Diseases 71, no. 2 (2011): 88. http://dx.doi.org/10.4046/trd.2011.71.2.88.

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Kim, Yu J., Ju-Young Kim, Jin Y. Yoon, et al. "Protective Effect of Aminophylline against Cigarette Smoke Extract-Induced Apoptosis in Human Lung Fibroblasts (MRC-5 Cells)." Basic & Clinical Pharmacology & Toxicology 109, no. 1 (2011): 17–22. http://dx.doi.org/10.1111/j.1742-7843.2011.00673.x.

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Nájera-Martínez, Minerva, Ethel A. García-Latorre, Elba Reyes-Maldonado, M. Lilia Domínguez-López, and Armando Vega-López. "Halomethane-induced cytotoxicity and cell proliferation in human lung MRC-5 fibroblasts and NL20-TA epithelial cells." Inhalation Toxicology 24, no. 11 (2012): 762–73. http://dx.doi.org/10.3109/08958378.2012.716871.

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Liu, Zhen, Jingyi Zhao, Xiao Liang, et al. "Dothiorelone derivatives from an endophyte Diaporthe pseudomangiferaea inhibit the activation of human lung fibroblasts MRC-5 cells." Fitoterapia 127 (June 2018): 7–14. http://dx.doi.org/10.1016/j.fitote.2018.04.009.

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Harrison, N. K., K. E. Dawes, O. J. Kwon, P. J. Barnes, G. J. Laurent, and K. F. Chung. "Effects of neuropeptides on human lung fibroblast proliferation and chemotaxis." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 2 (1995): L278—L283. http://dx.doi.org/10.1152/ajplung.1995.268.2.l278.

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An increase in subepithelial mesenchymal cells and associated connective tissue is a feature of bronchial asthma. We determined whether neuropeptides could modulate fibroblast activity, particularly with respect to proliferation and chemotaxis. Human lung fibroblasts were cultured with neurokinin A (NKA), substance P (SP), vasoactive intestinal peptide (VIP), and calcitonin-gene-related peptide (CGRP). After 48 h, fibroblast proliferation was measured by a colorimetric assay based on the uptake and subsequent release of methylene blue. The chemotactic response to neuropeptides was determined w

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Bové, Hannelore, Jens Devoght, Leentje Rasking, et al. "Combustion-derived particles inhibit in vitro human lung fibroblast-mediated matrix remodeling." Journal of Nanobiotechnology 16, no. 1 (2018): 82. https://doi.org/10.1186/s12951-018-0410-x.

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<strong>Background: </strong>The continuously growing human exposure to combustion-derived particles (CDPs) drives in depth investigation of the involved complex toxicological mechanisms of those particles. The current study evaluated the hypothesis that CDPs could affect cell-induced remodeling of the extracellular matrix due to their underlying toxicological mechanisms. The effects of two ultrafine and one fine form of CDPs on human lung fibroblasts (MRC-5 cell line) were investigated, both in 2D cell culture and in 3D collagen type I hydrogels. A multi-parametric analysis was employed.<stro

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Akers, Ian A., Maddy Parsons, Michael R. Hill, et al. "Mast cell tryptase stimulates human lung fibroblast proliferation via protease-activated receptor-2." American Journal of Physiology-Lung Cellular and Molecular Physiology 278, no. 1 (2000): L193—L201. http://dx.doi.org/10.1152/ajplung.2000.278.1.l193.

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Mast cells play a potentially important role in fibroproliferative diseases, releasing mediators including tryptase that are capable of stimulating fibroblast proliferation and procollagen synthesis. The mechanism by which tryptase stimulates fibroblast proliferation is unclear, although recent studies suggest it can activate protease-activated receptor (PAR)-2. We therefore investigated the role of PAR-2 in tryptase-induced proliferation of human fetal lung and adult lung parenchymal and airway fibroblasts and, for comparative purposes, adult dermal fibroblasts. Tryptase (0.7–70 mU/ml) induce

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Zhuang, Z. X., J. Z. Zhang, H. X. Huang, P. T. Tang, and B. Y. Zhang. "Nickel-induced oxidative stress, disruption of mitochondrial integrity and cytotoxicity in human lung fetal fibroblasts (MRC-5 cells)." Toxicology Letters 95 (July 1998): 56. http://dx.doi.org/10.1016/s0378-4274(98)80220-8.

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Goldsmith, K. T., R. B. Gammon, and R. I. Garver. "Modulation of bFGF in lung fibroblasts by TGF-beta and PDGF." American Journal of Physiology-Lung Cellular and Molecular Physiology 261, no. 6 (1991): L378—L385. http://dx.doi.org/10.1152/ajplung.1991.261.6.l378.

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Growth factors produced by alveolar macrophages are thought to promote the fibroblast proliferation within interstitial spaces of fibrotic lungs. This study investigated the possibility that the macrophage-produced growth factors might modulate the expression of basic fibroblast growth factor (bFGF) by lung fibroblasts. To evaluate this question, bFGF gene expression and protein production were evaluated in normal adult human lung fibroblast cell lines. Under normal culture conditions, the fibroblasts expressed the bFGF gene as two major transcripts (7.1, 3.7 kb). The addition of fetal calf se

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Kennedy, John C., Damir Khabibullin, Thomas Hougard, Julie Nijmeh, Wei Shi, and Elizabeth P. Henske. "Loss of FLCN inhibits canonical WNT signaling via TFE3." Human Molecular Genetics 28, no. 19 (2019): 3270–81. http://dx.doi.org/10.1093/hmg/ddz158.

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Abstract Lower lobe predominant pulmonary cysts occur in up to 90% of patients with Birt–Hogg–Dubé (BHD) syndrome, but the key pathologic cell type and signaling events driving this distinct phenotype remain elusive. Through examination of the LungMAP database, we found that folliculin (FLCN) is highly expressed in neonatal lung mesenchymal cells. Using RNA-Seq, we found that inactivation of Flcn in mouse embryonic fibroblasts leads to changes in multiple Wnt ligands, including a 2.8-fold decrease in Wnt2. This was associated with decreased TCF/LEF activity, a readout of canonical WNT activity

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UM, Yucel. "Investigation of the Cytotoxic Effect of Thorium on MRC-5 Cells." Advances in Pharmacology and Clinical Trials 8, no. 3 (2023): 1–3. http://dx.doi.org/10.23880/apct-16000221.

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Thorium is a naturally occurring radioactive metal belonging to the actinide group. It is found at low levels in water, soil, rocks, plants and animals. Thorium is used as a nuclear fuel in nuclear technology, and its compounds are used in industry, chemistry, mining, ceramic production and high-quality lens production. It also occurs naturally in different sources, including phosphate fertilizers used in agriculture. Its increasing role in the nuclear and defense industries poses a risk to human health as a result of occupational and accidental exposure. Thorium enters the body mainly through

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Janubová, Mária, and Ingrid Žitňanová. "Vplyv extraktu Pycnogenol® na stresom indukovanú predčasnú bunkovú senescenciu / Effect of Pycnogenol® extract on stress-induced premature cell senescence." Laboratórna Diagnostika XXVIII, no. 1/2023 (2023): 61–66. https://doi.org/10.5281/zenodo.7924947.

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<strong>Súhrn</strong> Bunková senescencia zohráva významnú úlohu v procese starnutia organizmu. Senescentné bunky hromadiace sa so zvy&scaron;ujúcim sa vekom v organizme prispievajú k zhor&scaron;eniu funkcií tkanív a orgánov a rozvoju ochorení spojených so starnutím. V na&scaron;ej práci sme sledovali vplyv extraktu Pycnogenol® na rozvoj stresom indukovanej predčasnej senescencie, nakoľko Pycnogenol® (extrakt z  kôry francúzsk

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Miki, Hiroyuki, Tadashi Mio, Sonoko Nagai, et al. "Glucocorticoid-induced contractility and F-actin content of human lung fibroblasts in three-dimensional culture." American Journal of Physiology-Lung Cellular and Molecular Physiology 278, no. 1 (2000): L13—L18. http://dx.doi.org/10.1152/ajplung.2000.278.1.l13.

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Fibroblast contractility plays a useful role in the wound healing process but contributes to architectural distortion in the lungs. Glucocorticoids (GCs) have been reported to reduce dermal fibroblast contractility, which may result in delaying wound healing, but the effects on lung fibroblasts are unknown. In this study, we examined how human lung fibroblast contractility is altered in the presence of GCs. Lung fibroblast cell lines ( n = 5) were established from normal parts of surgically resected lung tissue. The effects of GCs on contractility were investigated with a type I collagen gel c

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Maglova, Lilia M., William E. Crowe, Aníbal A. Altamirano, and John M. Russell. "Human cytomegalovirus infection stimulates Cl−/ HCO 3 − exchanger activity in human fibroblasts." American Journal of Physiology-Cell Physiology 275, no. 2 (1998): C515—C526. http://dx.doi.org/10.1152/ajpcell.1998.275.2.c515.

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The effects of human cytomegalovirus (HCMV) infection on Cl−/[Formula: see text]exchanger activity in human lung fibroblasts (MRC-5 cells) were studied using fluorescent, ion-sensitive dyes. The intracellular pH (pHi) of mock- and HCMV-infected cells bathed in a solution containing 5% CO2-25 mM[Formula: see text] were nearly the same. However, replacement of external Cl−with gluconate caused an H2DIDS-inhibitable (100 μM) increase in the pHi of HCMV-infected cells but not in mock-infected cells. Continuous exposure to hyperosmotic external media containing CO2/[Formula: see text]caused the pHi

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Tadić, Julijana D., Jelena M. Lađarević, Željko J. Vitnik, et al. "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity." Dyes and Pigm 187, March (2021): 109123. https://doi.org/10.1016/j.dyepig.2020.109123.

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Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant

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Wójcik-Pszczoła, Katarzyna, Grażyna Chłoń-Rzepa, Agnieszka Jankowska та ін. "A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling". International Journal of Molecular Sciences 21, № 11 (2020): 4008. http://dx.doi.org/10.3390/ijms21114008.

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Phosphodiesterase (PDE) inhibitors are currently a widespread and extensively studied group of anti-inflammatory and anti-fibrotic compounds which may find use in the treatment of numerous lung diseases, including asthma and chronic obstructive pulmonary disease. Several PDE inhibitors are currently in clinical development, and some of them, e.g., roflumilast, are already recommended for clinical use. Due to numerous reports indicating that elevated intracellular cAMP levels may contribute to the alleviation of inflammation and airway fibrosis, new and effective PDE inhibitors are constantly b

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Roa, Wilson, Bryan Brunet, Linghong Guo, et al. "Identification of a new microRNA expression profile as a potential cancer screening tool." Clinical & Investigative Medicine 33, no. 2 (2010): 124. http://dx.doi.org/10.25011/cim.v33i2.12351.

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Purpose: Small non-coding microRNAs (miRNAs) are key components of cancer development and are considered as potential biomarkers for cancer diagnosis and treatment monitoring. This study investigated miRNA expression profiles of human cancer cells in order to develop a screening method for lung cancer. Methods: A series of lung cancer related miRNAs (miR-21, miR-145, miR-155, miR-205, miR-210, miR-92, miR-17-5p, miR-143, miR-182, miR-372, let-7a) were selected as candidates for miRNA expression profiles of human lung cancer cell lines (A549, SK-mes-1). MicroRNA u6 was the endogenous control. C

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Pantelić, Nebojša, Tatjana P. Stanojković, Bojana B. Zmejkovski, Goran N. Kaluđerović, and Tibor J. Sabo. "Antiproliferative Activity of Gold(III) Complexes with Esters of Cyclohexyl-Functionalized Ethylenediamine-N,N’-Diacetate." Serbian Journal of Experimental and Clinical Research 18, no. 4 (2017): 289–94. http://dx.doi.org/10.1515/sjecr-2017-0067.

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AbstractSix gold(III) complexes with esters of cyclohexyl-functionalized ethylenediamine-N,N’-diacetate, general formula [AuCl2{(S,S)-R2eddch}]PF6, [(S,S)-eddch = (S,S)-ethylenediamine-N,N’-di-2-(3-cyclohexyl)propanoate, R = Me, Et, n-Pr, n-Bu, i-Bu, i-Am, 1–6, respectively], were tested against cancer cell lines such as human melanoma Fem-x, human colon carcinoma LS174T and non-small cell lung carcinoma A549 as well as a non-cancerous human embryonic lung fibroblasts MRC-5 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with the aim of assessing in vitro antitum

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Klyushova, L. S., V. A. Vavilin, and A. Yu Grishanova. "The cytotoxic and antiproliferative properties of ruthenium nitrosyl complexes and their modulation effect on cytochrome P450 in the HepG2 cell line." Biomeditsinskaya Khimiya 70, no. 1 (2024): 33–40. http://dx.doi.org/10.18097/pbmc20247001033.

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Ruthenium nitrosyl complexes are actively investigated as antitumor agents. Evaluation of potential interactions between cytochromes P450 (CYPs) with new compounds is carried out regularly during early drug development. In this study we have investigated the cytotoxic and antiproliferative activities of ruthenium nitrosyl complexes with methyl/ethyl esters of nicotinic and isonicotinic acids and γ-picoline against 2D and 3D cultures of human hepatocellular carcinoma HepG2 and non-cancer human lung fibroblasts MRC-5, assessed their photoinduced activity at λrad = 445 nm, and also evaluated thei

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Ivankin, Dmitry I., Tatyana E. Kornienko, Marina A. Mikhailova, et al. "Novel TDP1 Inhibitors: Disubstituted Thiazolidine-2,4-Diones Containing Monoterpene Moieties." International Journal of Molecular Sciences 24, no. 4 (2023): 3834. http://dx.doi.org/10.3390/ijms24043834.

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Tyrosyl-DNA-phosphodiesterase 1 (TDP1) is a promising target for antitumor therapy; the use of TDP1 inhibitors with a topoisomerase 1 poison such as topotecan is a potential combination therapy. In this work, a novel series of 3,5-disubstituted thiazolidine-2,4-diones was synthesized and tested against TDP1. The screening revealed some active compounds with IC50 values less than 5 μM. Interestingly, compounds 20d and 21d were the most active, with IC50 values in the submicromolar concentration range. None of the compounds showed cytotoxicity against HCT-116 (colon carcinoma) and MRC-5 (human l

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El-Atawy, Mohamed A., Najla A. Alshaye, Nada Elrubi, Ezzat A. Hamed, and Alaa Z. Omar. "Pyrimidines-Based Heterocyclic Compounds: Synthesis, Cytoxicity Evaluation and Molecular Docking." Molecules 27, no. 15 (2022): 4912. http://dx.doi.org/10.3390/molecules27154912.

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A variety of structurally different pyrimidines were synthesized. Elemental analysis, FT-IR, 1H NMR, and 13C NMR spectroscopy were used to confirm the chemical structures of all prepared compounds. The synthesized pyrimidines were screened against the growth of five human cancer cell lines (prostate carcinoma PC3, liver carcinoma HepG-2, human colon cancer HCT-116, human breast cancer MCF-7, human lung cancer A-549), and normal human lung fibroblasts (MRC-5) using MTT assay. Most of the screened pyrimidines have anti-proliferative activity on the growth of the PC3 cell line. Compounds 3b and 3

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Yin, Zhaohong, and Mitchell A. Watsky. "Chloride channel activity in human lung fibroblasts and myofibroblasts." American Journal of Physiology-Lung Cellular and Molecular Physiology 288, no. 6 (2005): L1110—L1116. http://dx.doi.org/10.1152/ajplung.00344.2004.

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It is well established that transforming growth factor (TGF)-β stimulates human lung fibroblasts (HLF) to differentiate into myofibroblasts. We characterized lysophosphatidic acid (LPA)-activated Cl− channel current ( ICl-LPA) in cultured human lung fibroblasts and myofibroblasts and investigated the influence of ICl-LPA on fibroblast-to-myofibroblast differentiation. We recorded ICl-LPA using the amphotericin perforated-patch technique. We activated ICl-LPA using LPA or sphingosine-1-phosphate. We determined phenotype by Western blotting and immunohistochemistry using an anti-α-smooth muscle

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Stojanović, Stevan D., Maximilian Fuchs, Jan Fiedler, et al. "Comprehensive Bioinformatics Identifies Key microRNA Players in ATG7-Deficient Lung Fibroblasts." International Journal of Molecular Sciences 21, no. 11 (2020): 4126. http://dx.doi.org/10.3390/ijms21114126.

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Background: Deficient autophagy has been recently implicated as a driver of pulmonary fibrosis, yet bioinformatics approaches to study this cellular process are lacking. Autophagy-related 5 and 7 (ATG5/ATG7) are critical elements of macro-autophagy. However, an alternative ATG5/ATG7-independent macro-autophagy pathway was recently discovered, its regulation being unknown. Using a bioinformatics proteome profiling analysis of ATG7-deficient human fibroblasts, we aimed to identify key microRNA (miR) regulators in autophagy. Method: We have generated ATG7-knockout MRC-5 fibroblasts and performed

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