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Journal articles on the topic 'Mycobacterium Tuberculi'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Pandey, Saumya. "Toll-like Receptors and Artificial Intelligence in Fertility Management: Clinical Research Snapshot in United States of America and Asia-Pacific India Genetic Landscapes." Clinical Research and Reports 2, no. 1 (2024): 1–3. http://dx.doi.org/10.59657/2995-6064.brs.23.003.

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Dissecting the intricate “maternal-fetal immune cross-talks” in the complex etiopathogenesis of reproductive endocrinology disorders primarily Mycobacterium tuberculi mediated female infertility and Chlamydia trachomatis mediated male infertility in genetically disparate susceptible “at-risk” cohorts of heterogeneous population-pools by amalgamating precision-based therapeutic targeting of Ceramide-Wnt/Frizzled-Toll like receptors-autophagy biochemical/metabolic signaling cascades with Artificial Intelligence (AI) offers fascinating healthcare management avenues in timeline-driven pragmatic, e

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M.P. Narmadha, A. S. Ushananthini, A. Murugan, R. Saravanan, and D. Sridhar. "Assessment of DNA damage in pulmonary tuberculosis patients by single cell gel electrophoresis / comet assay." International Journal of Fundamental and Applied Sciences (IJFAS) 1, no. 3 (2012): 27–32. http://dx.doi.org/10.59415/ijfas.v1i3.27.

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Background: The role of antitubercular drugs as anti bacterial agents on mycobacterium tuberculi is established.However their impact on human cell is not much explored. Objective: The present study was aimed to determine theextent of cell damage in Pulmonary Tuberculosis patients with the help of comet assay. This is a prospective studycarried out in Tiruchengodu Govt. Hospital from September 2011 to April 2012. Blood samples were collected from100 subjects [Healthy volunteers (Go), newly diagnosed TB patients (G1), Three months treated TB patients (G2), Sixmonths treated TB patients (G3). n=2

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Pujari, Dr Shridhar G. "A Study to Assess the Impact of Structured Teaching Programme on Knowledge Regarding Self Protection Practices against Tuberculosis among Staff Nurses Working at H.S.K Medical College Hospital and Research Centre Bagalkot." Scholars Journal of Applied Medical Sciences 13, no. 03 (2025): 658–62. https://doi.org/10.36347/sjams.2025.v13i03.009.

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Background of the study: Throughout the world, poor people, health care workers and those from disadvantaged social groups suffer most illness and die sooner. Tuberculosis continues to rank among the world’s most serious health problems, despite the remarkable achievements of discovering effective diagnostics measures. The bacillus causing Tuberculosis, Mycobacterium tuberculi was identified and described on March 24, 1882 by Dr Robert Koch. Tuberculosis is infectious disease characterized by persistent cough. The organism primarily affects and causes pulmonary tuberculosis, and as the disease

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Kasikovic-Lecic, Svetlana, Slobodan Pavlovic, Vesna Kuruc, and Miroslav Ilic. "Immunological assays in the diagnosis of tuberculosis infection at the beginning of 21st century." Srpski arhiv za celokupno lekarstvo 138, no. 7-8 (2010): 515–17. http://dx.doi.org/10.2298/sarh1008515k.

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A subject infected by tubercle bacilli may immediately develop the disease (primary tuberculosis), witch occurs in a few number of infected persons, or may overcome the primary infection (a latent carrier of the infection). Nowadays there are about two milliard people with a latent tubercular infection. About 10% of them will develop active tuberculosis during their lifetime. Detection of latent tuberculosis and its treatment decreases the risk of developing the active form of the disease. Until recently, the tuberculin test was a single screening method to identify the subjects with tubercula

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Tiwari, Mohit Kumar, and Pratibha Gupta. "In vitro Study on, Effect of Anti Tubercular Drugs on Mycobacterium Lucknowense, sp. nov. Isolated from Indian Frog Rana Tigerina." Anusandhaan - Vigyaan Shodh Patrika 8, no. 1 (2020): 49–53. https://doi.org/10.22445/avsp.v8i1.8.

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Mycobacteria are the bacilli responsible for causing tuberculosis and leprosy like diseases. Tuberculosis is a important global problem, caused by Mycobacterium tuberculosis. Interrupted chemotherapy led to spread of drug resistant mycobacterial infections, which is now most burning problem all over the world. A typical Mycobacteria and other members of genus which can cause typical tuberculosis like symptoms and extra pulmonary mycobacterial infections in almost all parts of human body and also in other animals from Pisces, Reptiles, Birds and Mammalia. Infected man can spread disease in anim

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FINE, P. E. M., S. FLOYD, J. L. STANFORD, et al. "Environmental mycobacteria in northern Malawi: implications for the epidemiology of tuberculosis and leprosy." Epidemiology and Infection 126, no. 3 (2001): 379–87. http://dx.doi.org/10.1017/s0950268801005532.

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More than 36000 individuals living in rural Malawi were skin tested with antigens derived from 12 different species of environmental mycobacteria. Most were simultaneously tested with RT23 tuberculin, and all were followed up for both tuberculosis and leprosy incidence. Skin test results indicated widespread sensitivity to the environmental antigens, in particular to Mycobacterium scrofulaceum, M. intracellulare and one strain of M. fortuitum. Individuals with evidence of exposure to ‘fast growers’ (i.e. with induration to antigens from fast growers which exceeded their sensitivity to tubercul

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Prytychenko, A. A., A. P. Lysenko, M. V. Kuchvalski, and E. L. Krasnikova. "Allergic activity and specificity of tuberculin preparations with 30-50% of weakly secreted mycobacterial antigens of tuberculosis." Proceedings of the National Academy of Sciences of Belarus. Agrarian Series 58, no. 4 (2020): 472–82. http://dx.doi.org/10.29235/1817-7204-2020-58-4-472-482.

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Bovine tuberculosis remains a global problem. An intracutaneous test with tuberculin is the main method for determining the status of herds, which poses special requirements for the activity and specificity. The basis of cotemporal tuberculins are antigens of tuberculosis mycobacteria easily secreted to the liquid synthetic medium during growth, but a range of antigens with a low secretion index are in composition of tuberculins in small quantities. The purpose of the research is to obtain weakly secreted antigens from a production waste – autoclaved bacterial mass of production strain of tube

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8

Dr., Sonali Banpure* Bhalekar Pournima Ingale Pramod Matade Siddhesh Mhase Pratiksha. "Design, In Silico Evaluation, Synthesis and Biological Evaluation Of 4-Nitroimidazole Derivatives." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1621–27. https://doi.org/10.5281/zenodo.15381646.

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This study aimed to design and evaluate novel antitubercular agents using computational methods. Four derivatives of isoniazid and acyclovir were designed and subjected to molecular docking studies against the 3IX2 protein. The results showed that the first derivative had the lowest binding energy (-11.58) and formed three hydrogen bonds with the protein. ADMET analysis was performed to evaluate the pharmacokinetic properties of the designed compounds. Based on the molecular docking and ADMET analysis, the first derivative was identified as the most promising compound for further development a

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Pavlik, I., L. Matlova, L. Dvorska, Shitaye JE, and I. Parmova. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000–2004." Veterinární Medicína 50, No. 7 (2012): 281–90. http://dx.doi.org/10.17221/5625-vetmed.

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Bovine tuberculosis was last detected in cattle and pigs in the CzechRepublicin 1995. Since March,&nbsp;31, 2004(Commission Decision No. 2004/320/EC) the Czech Republichas been included amongst states free from bovine tuberculosis within the European Union. The purpose of the present study was to evaluate results of intravital and post-mortem diagnosis of mycobacterial infections in slaughtered cattle and pigs from 2000 to 2004. When bovine tuberculosis in cattle was investigated, a tuberculin skin test with bovine tuberculin was performed every year and a skin test with avian tuberculin w

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10

Davis, William C., Gaber S. Abdellrazeq, Asmaa H. Mahmoud, et al. "Advances in Understanding of the Immune Response to Mycobacterial Pathogens and Vaccines through Use of Cattle and Mycobacterium avium subsp. paratuberculosis as a Prototypic Mycobacterial Pathogen." Vaccines 9, no. 10 (2021): 1085. http://dx.doi.org/10.3390/vaccines9101085.

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Lack of understanding of the immune response to mycobacterial pathogens has impeded progress in development of vaccines. Infection leads to development of an immune response that controls infection but is unable to eliminate the pathogen, resulting in a persistent infection. Although this puzzle remains to be solved, progress has been made using cattle as a model species to study the immune response to a prototypic mycobacterium, Mycobacterium a. paratuberculosis (Map). As chronicled in the review, incremental advances in characterizing the immune response to mycobacteria during the last 30 ye

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Asiniwasis, Rachel, Maha T. Dutil, and Scott Walsh. "Molluscum-Like Papules as a Presentation of Early Papulonecrotic Tuberculid in Association with Nodular Tuberculid in a Male with Asymptomatic Active Pulmonary Tuberculosis." Journal of Cutaneous Medicine and Surgery 19, no. 2 (2015): 159–62. http://dx.doi.org/10.2310/7750.2014.14035.

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Background/Objectives The clinical and histopathologic findings of a rare simultaneous occurrence of papulonecrotic tuberculid and nodular tuberclid in a patient with active but asymptomatic pulmonary tuberculosis are presented. Papulonecrotic tuberculid was observed at a very early stage, presenting as molluscum-like lesions. This has been described once in the literature. This was observed in conjunction with lesions compatible with the rare clinicopathologic variant of nodular tuberculid. Critical to the diagnosis of active pulmonary tuberculosis was the use of induced sputum testing, which

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Gooding, Travis M., Paul D. R. Johnson, Dianne E. Campbell, et al. "Immune Response to Infection withMycobacterium ulcerans." Infection and Immunity 69, no. 3 (2001): 1704–7. http://dx.doi.org/10.1128/iai.69.3.1704-1707.2001.

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ABSTRACT Mycobacterium ulcerans is a slow-growing, acid-fast bacillus that causes chronic necrotizing skin ulcers known as Buruli ulcers. Previously reported information on immunity to this mycobacterium is limited. We examined immune responses to M. ulcerans and M. bovis BCG in patients with M. ulcerans disease and in 20 healthy control subjects (10 tuberculin test positive and 10 tuberculin test negative). Cell-mediated immunity was assessed by stimulating peripheral blood mononuclear cells (PBMC) with whole mycobacteria and then measuring PBMC proliferation and the production of gamma inter

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Lysenko, AP, L. Broxmeyer, VV Vlasenko, PA Krasochko, AP Lemish, and EA Krasnikova. "Further Evidence for Cancer as a Cell-Wall- Deficient Mycobacterial Disease." Journal of MPE Molecular Pathological Epidemiology 1, no. 1:5 (2017): 12. https://doi.org/10.5281/zenodo.894195.

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In 2014, Buehring reported that Bovine Leukemic Virus (BLV), a common oncogenic retrovirus of cattle, was present in some humans, primarily localized to the breast epithelium ― the very cell type from which most breast malignancies arise. By 2015, there appeared data (Buehring, 2015) supporting that as many as 37% of human breast cancer cases could be attributable to BLV exposure. But if recent estimates suggest over 83% of U.S. dairy operations are currently positive for BLV, they also show that approximately 68% are positive for cell-wall-deficient Mycobacterium avium subspecies paratubercul

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Szacawa, Ewelina, Nina Kozieł, Sylwia Brzezińska, et al. "Laboratory Diagnosis of Animal Tuberculosis in Tracing Interspecies Transmission of Mycobacterium bovis." Pathogens 14, no. 5 (2025): 459. https://doi.org/10.3390/pathogens14050459.

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Mycobacterium is one of the most dangerous pathogens of both animals and humans. Bovine tuberculosis (BTB) is a disease caused by mycobacteria belonging to the Mycobacterium tuberculosis complex (MTBC), which spreads mainly among domestic cattle but also to mammals other than cattle. The transmission of MTBC between different species requires research and epidemiological investigations to control its spread. When multiple species are a reservoir of infection, it poses a significant public health and veterinary concern. In this study, the diagnosis of alpaca, cattle, horses, dogs, a sheep and a

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Converse, P. J., A. M. Dannenberg, T. Shigenaga, et al. "Pulmonary Bovine-Type Tuberculosis in Rabbits: Bacillary Virulence, Inhaled Dose Effects, Tuberculin Sensitivity, and Mycobacterium vaccae Immunotherapy." Clinical Diagnostic Laboratory Immunology 5, no. 6 (1998): 871–81. http://dx.doi.org/10.1128/cdli.5.6.871-881.1998.

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ABSTRACT This report elucidates four aspects of the immunology of pulmonary tuberculosis produced in rabbits: (i) the virulence of bovine-type tubercle bacilli, strain Ravenel S, (ii) systemic factors influencing the generation of visible primary pulmonary tubercles, (iii) differences in tuberculin sensitivity of rabbits and humans, and (iv) the effect of Mycobacterium vaccae immunotherapy on cavitary tuberculosis. Laboratory strain Ravenel S (ATCC 35720) was not fully virulent. Fully virulent strains produce one visible primary pulmonary tubercle for each three bacillary units inhaled. Strain

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Zavgorodniy, A. I., V. V. Bilushko, S. A. Pozmogova, et al. "Determination of the causes of allergic reactions to tuberculin in cattle." Veterinary Medicine: inter-departmental subject scientific collection, no. 107 (November 8, 2021): 30–36. http://dx.doi.org/10.36016/vm-2021-107-5.

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The article presents the results of cattle examining in four free from tuberculosis livestock farms during 2020–2021. Samples of biological material were collected and studied in the Laboratory for tuberculosis study of the NSC “IECVM”. The causes of allergic reactions to mycobacterial allergens were established by a comprehensive method. The study was aimed to conduct epizootological monitoring and to determine the causes of positive tuberculin skin tests in cattle in four free from tuberculosis farms. These farms were located in different regions of Ukraine. Epizootological, clinical, allerg

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Baratov, M. O. "Towards improved differential diagnostics of bovine tuberculosis in the Republic of Dagestan." Veterinary Science Today 14, no. 2 (2025): 164–70. https://doi.org/10.29326/2304-196x-2025-14-2-164-170.

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Introduction. Non-specific tuberculin reactions are among the most critical challenges in tuberculosis diagnosis, with their incidence increasing annually. Given the complex epidemiological challenges, improving bovine tuberculosis diagnostics is critically important.Objective. Development of effective comprehensive differential bovine tuberculosis diagnosis and introduction of improved techniques for the infection detection in farms with different animal health statuses in the Republic of Dagestan.Materials and methods. 1,670 cattle were subjected to tuberculin testing; 3,502 serum samples we

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Ali, Ali Mohammedain, and Mubarak S. Elkarsany. "Comparison of Ziehl-Neelsen Microscopy Technique and Genexpert in the Diagnosis of Tuberculosis in Sudanese Patients." Pakistan Journal of Medical and Health Sciences 16, no. 9 (2022): 648–50. http://dx.doi.org/10.53350/pjmhs22169648.

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Aim: The quality of tuberculosis diagnosis service is indispensable for early infection control and preventing unnecessary anti-tubercular therapy use. TB diagnosis in Sudan mainly relies on detecting tubercule bacilli using the Ziehl-Neelsen (ZN) staining technique. Thus, the current study aimed to compare the ZN microscopy with GeneXpert in diagnosing pulmonary tuberculosis (PTB) in TB laboratories center in Khartoum state, Sudan. Methods: A cross-sectional study was carried out at the TB laboratories diagnosis center in Khartoum state from April 2019 to January 2020. A total of 183 sputum s

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Cvetnic, Z., S. Spicic, V. Katalinic-Jankovic, et al. "Mycobacterium capraeinfection in cattle and pigs on one family farm inCroatia: a case report." Veterinární Medicína 51, No. 11 (2012): 523–31. http://dx.doi.org/10.17221/5586-vetmed.

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An outbreak of tuberculosis among bovines and pigs caused by Mycobacterium caprae is described in this paper. After tuberculin skin tests with bovine purified protein derivates (PPD) six cattle and one sow, own by a small family farm, tested positive whilst three pigs were suspected in 2004. All animals were euthanised and checked for gross pathological lesions. Generalised lesions were found in five cattle and two sows; however one calf and two gilts had lesions that were localised in the submandibular lymph nodes. Mycobacteria were isolated from tissue samples of six cattle and four pigs. My

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Corneli, Sara, Laura Corte, Luca Roscini, et al. "Spectroscopic Characterization of Bovine, Avian and Johnin Purified Protein Derivative (PPD) with High-Throughput Fourier Transform InfraRed-Based Method." Pathogens 8, no. 3 (2019): 136. http://dx.doi.org/10.3390/pathogens8030136.

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Tuberculins purified protein derivatives (PPDs) are obtained by precipitation from heat treated mycobacteria. PPDs are used in diagnosis of mycobacterial infections in humans and animals. Bovine PPD (PPDB) is obtained from Mycobacterium bovis (Mycobacterium tuberculosis complex), while Avian PPD (PPDA) and Johnin PPD (PPDJ) are extracted, respectively, from Mycobacterium avium and M. avium subsp. paratuberculosis (M. avium complex). PPDB and PPDA are used for bovine tuberculosis diagnosis, while PPDJ is experimentally used in the immunodiagnosis of paratuberculosis. Although PPDs date back to

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Boritsch, Eva C., Varun Khanna, Alexandre Pawlik, et al. "Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria." Proceedings of the National Academy of Sciences 113, no. 35 (2016): 9876–81. http://dx.doi.org/10.1073/pnas.1604921113.

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Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, frag

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Pandey, Saumya. "TOLL-LIKE RECEPTOR-2 AND TISSUE INHIBITOR OF MATRIX METALLOPROTEINASE-2 GENETIC VARIANTS AS PREDICTORS OF TOBACCO-MEDIATED FEMALE INFERTILITY AMONGST MYCOBACTERIUM TUBERCULI-POSITIVE ASIAN INDIAN COHORT." Fertility and Sterility 114, no. 3 (2020): e541. http://dx.doi.org/10.1016/j.fertnstert.2020.09.061.

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Lyashchenko, Konstantin, Claudia Manca, Roberto Colangeli, Anna Heijbel, Alan Williams, and Maria Laura Gennaro. "Use of Mycobacterium tuberculosisComplex-Specific Antigen Cocktails for a Skin Test Specific for Tuberculosis." Infection and Immunity 66, no. 8 (1998): 3606–10. http://dx.doi.org/10.1128/iai.66.8.3606-3610.1998.

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ABSTRACT The tuberculin skin test currently used to diagnose infection withMycobacterium tuberculosis has poor diagnostic value, especially in geographic areas where the prevalence of tuberculosis is low or where the environmental burden of saprophytic, nontuberculous mycobacteria is high. Inaccuracy of the tuberculin skin test often reflects a low diagnostic specificity due to the presence in tuberculin of antigens shared by many mycobacterial species. Thus, a skin test specific for tuberculosis requires the development of new tuberculins consisting of antigens specific to M. tuberculosis. We

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Dey, Rishita, Sisir Nandi, Asmita Samadder, Aaruni Saxena, and Anil Kumar Saxena. "Exploring the Potential Inhibition of Candidate Drug Molecules for Clinical Investigation Based on their Docking or Crystallographic Analyses against M. tuberculosis Enzyme Targets." Current Topics in Medicinal Chemistry 20, no. 29 (2020): 2662–80. http://dx.doi.org/10.2174/1568026620666200903163921.

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Tuberculosis (TB) is a devastating disease responsible for millions of humans’ deaths worldwide. It is caused by a mycobacterial organism, the tubercle bacillus or Mycobacterium tuberculosis. Although TB can be treated, cured and can be prevented if patients take prescribed medicines, scientists have never come close to wiping it out due to a sharp rise in the incidence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) mycobacterium strains. Due to long regimen treatment and emergence of MDR and XDR-TB, it is urgent to re-engineer and reposition old drugs for developing new ant

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Savova-Lalkovska, Tanya, Violeta Valcheva, Albena Dimitrova, Hristo Najdenski, and Magdalena Bonovska. "Advantages of Real-time PCR and RD4-PCR as Molecular Methods for Rapid Detection of Bovine Tuberculosis in Bulgaria." Proceedings of the Bulgarian Academy of Sciences 75, no. 11 (2022): 1696–704. http://dx.doi.org/10.7546/crabs.2022.11.18.

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Representatives of Mycobacterium tuberculosis complex (MTBC) are potential causative agents of tuberculosis in animals and humans, and Mycobacterium bovis is considered a major one among domestic and wild ruminants. In the last twenty years, the role of another representative of MTBC – Mycobacterium caprae, has been proven in the countries of Central and Southern Europe. Study sample included 121 diagnostic materials from lymph nodes and lungs from cattle, positively and doubtfully PPD tuberculin-reacted, originating from 6 farms belonging to the 4 regions in Northern and Southern Bulgaria. Th

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Khayyam, Salik Umer, Khalid Umer Khayyam, Sazina Muzammil, and Krishna Mohan. "Clinical Profile of Lichen Scrofulosorum, a Cutaneous Manifestation with Focus on Tubercular Lymphadenitis in a 15-Year-Old Male- A Case Report." Scholars Journal of Medical Case Reports 12, no. 03 (2024): 385–88. http://dx.doi.org/10.36347/sjmcr.2024.v12i03.038.

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Lichen scrofulosorum (LS) or tuberculosis cutis lichenoides is a rare form of tuberculid, an immunological reaction to the Mycobacterium tuberculosis bacilli which may be occult or present with primary focus elsewhere in the body. Other typical recognized forms of tuberculid include erythema induratum and papulonecrotic tuberculid. We report a case of LS with tubercular mediastinal lymphadenopathy in a 15- year-old male who presented with asymptomatic, recurrent, multiple papular rashes on the proximal side of both arms, sides of trunk, chest, stomach area, and back, for the last 5 months. Ski

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Colangeli, Roberto, John S. Spencer, Pablo Bifani, et al. "MTSA-10, the Product of the Rv3874 Gene of Mycobacterium tuberculosis, Elicits Tuberculosis-Specific, Delayed-Type Hypersensitivity in Guinea Pigs." Infection and Immunity 68, no. 2 (2000): 990–93. http://dx.doi.org/10.1128/iai.68.2.990-993.2000.

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ABSTRACT In a search for new skin test reagents specific for tuberculosis, we found that the antigen encoded by gene Rv3874 of Mycobacterium tuberculosis elicited delayed-type hypersensitivity in M. tuberculosis-infected guinea pigs but not in control animals immunized with Mycobacterium bovis bacillus Calmette-Guérin (BCG) or Mycobacterium avium. The antigen, which was named MTSA-10 (for M. tuberculosis-specific antigen 10), is a prime candidate for a component of a new tuberculin that will allow discrimination by a skin test of latent M. tuberculosis infection from vaccination with BCG or f

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Zavgorodniy, A. I., V. V. Bilushko, M. V. Kalashnyk, et al. "Diagnosis of bovine tuberculosis in free from tuberculosis farms of Ukraine." Veterinary Medicine: inter-departmental subject scientific collection, no. 106 (November 30, 2020): 48–55. http://dx.doi.org/10.36016/vm-2020-106-9.

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The article presents the results of studies of cattle in five free from tuberculosis livestock farms during 2016–2020. Samples of biological material were collected and studied in the Laboratory for Tuberculosis Study of NSC “IECVM”. The causes of allergic reactions to mycobacterial allergens were established by a comprehensive method. The aim of the study was to conduct epizootological monitoring and to determine the causes of positive tuberculin skin test in cattle in five farms, which are free from tuberculosis. These farms are located in different regions of Ukraine. Epizootological, clini

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Parvez, Masud, Md Mohiuddin, Md Zahid Hassan, Farooque Ahmad, and J. Ashraful Haq. "Diagnosis of Tubercular Lymphadenitis by PCR of Fine Needle Aspirates." Ibrahim Medical College Journal 6, no. 2 (2013): 46–49. http://dx.doi.org/10.3329/imcj.v6i2.14728.

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A definitive and accurate diagnosis of tubercular lymphadenitis is important for its proper management. Fine needle aspiration cytology (FNAC) is an easy procedure for collection of material for cytopathological and bacteriological examination. But the detection rate of M. tuberculosis from the aspirated material is low with Ziehl-Neelson (Z-N) stain and even with culture. Polymerase chain reaction (PCR) is a rapid method for diagnosis of tuberculosis from various clinical samples. In the present study, PCR was employed for the detection of mycobacterial DNA sequences in fine needle aspirates

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Patil, Avinash, Harleen Duggal, Kamini T. Bagul, et al. "Synthesis of New 3-Arylaminophthalides and 3-Indolyl-phthalides using Ammonium Chloride, Evaluation of their Anti-Mycobacterial Potential and Docking Study." Combinatorial Chemistry & High Throughput Screening 23, no. 8 (2020): 723–39. http://dx.doi.org/10.2174/1386207323666200422082754.

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Objective: The study aims at the derivatization of “Phthalides” and synthesizes 3- arylaminophthalides & 3-indolyl-phthalides compounds, and evaluates their anti-tubercular and antioxidant activities. The study has also intended to employ the in silico methods for the identification of possible drug targets in Mycobacterium and evaluate the binding affinities of synthesized compounds. Methods: This report briefly explains the synthesis of phthalide derivatives using ammonium chloride. The synthesized compounds were characterized using spectral analysis. Resazurin Microtiter Assay (REMA) pl

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Ramos, Joelson Marcolino, Marcos Bryan Heinemann, José Soares Ferreira Neto, et al. "Isolation and identification of Mycobacterium bovis in milk from cows in northeastern Brazil." Ciência Rural 46, no. 12 (2016): 2166–69. http://dx.doi.org/10.1590/0103-8478cr20160416.

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ABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion.

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Kowe, Priyanka, Sanket Anil Surushe, Grishma Govind Kale, and Rachita S. Dhurat. "Papulonecrotic tuberculid with an atypical presentation." Cosmoderma 4 (August 1, 2024): 85. http://dx.doi.org/10.25259/csdm_83_2024.

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The tuberculids constitute the immune-mediated non-infectious cutaneous hypersensitivity reaction pattern to the underlying focus of Mycobacterium tuberculosis antigen in patients with good cell-mediated immunity. Papulonecrotic tuberculid (PNT) is a true tuberculid that usually occurs secondary to the underlying focus of infection which may be in the lymph nodes, lungs, bone, or rarely in other systems. It manifests as asymptomatic symmetric eruptions of erythematous papules with central areas of ulceration and crusting that heals with varioliform scars. PNT presenting as a generalized follic

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Larsson, LO, M. Magnusson, BE Skoogh, and A. Lind. "Sensitivity to sensitins and tuberculin in Swedish children. IV. The influence of BCG-vaccination." European Respiratory Journal 5, no. 5 (1992): 584–86. http://dx.doi.org/10.1183/09031936.93.05050584.

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BCG-vaccinated schoolchildren, 8-9 yrs of age, were simultaneously tested on separate arms with tuberculin and a sensitin. Using the 6 mm cut-off they reacted in 49% to two tuberculin units purified protein derivative (PPD RT23), in 67% to 0.1 micrograms Mycobacterium avium sensitin RS10, and in 58% to 0.1 micrograms M. scrofulaceum sensitin RS95. The corresponding figures for non-BCG-vaccinated schoolchildren of the same age tested at the same time were 3, 25 and 32%, respectively. The results indicate a stimulating influence of BCG-vaccination on tuberculin and sensitin reactivity. Since the

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Mallick, Bipadabhanjan, Harshal S. Mandavdhare, Sourabh Aggarwal, Harjeet Singh, Usha Dutta, and Vishal Sharma. "Mycobacterial chylous ascites: report of three cases and systematic review." Therapeutic Advances in Infectious Disease 5, no. 4 (2018): 69–75. http://dx.doi.org/10.1177/2049936118772754.

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Background: Chylous ascites is an uncommon presentation of mycobacterial infection. Methods: We report three cases of tubercular chylous ascites, and in addition, we performed a systematic review of the published literature for the clinical presentation, treatment, and outcomes of mycobacterial chylous ascites. We followed the PRISMA guidelines for the systematic review. Results: A total of 33 cases (including three of ours) were included. The mean age of the reported cases was 32.54 ± 17.56 years, and a male predominance (76%) was noted. The predominant clinical features were abdominal disten

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Naik*, Vaidehee Milind, Hoogar M. B., Atul Jain, et al. "Erythema induratum: a rare case with unusual presentation." International Journal of Bioassays 6, no. 03 (2017): 5313. http://dx.doi.org/10.21746/ijbio.2017.03.006.

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Tuberculosis, still one of the most common infectious diseases globally, manifests primarily as pulmonary tuberculosis. Extra-pulmonary tuberculosis can occur in other sites including skin. Cutaneous tuberculosis, comprising merely 1-2% of all forms of tuberculosis, has been classified into various morphological variants. One of the morphological variants of cutaneous tuberculosis is erythema induratum, a tuberculid, which is caused by hematogenous spread of tuberculosis, the pathogenesis of which is due to hypersensitive reaction against the mycobacterial antigens. The skin biopsy findings of

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Ozmen, Semih, Mehmet Omer Kiristioglu, Gamze Ucan Gunduz, Ozgur Yalcinbayir, and Oner Gelisken. "An Unusual Cause of Serpiginous-Like Choroiditis. Association With Urinary Mycobacterium Fortuitum Infection?" POINT OF VIEW. EAST – WEST, no. 2 (May 12, 2023): 66–70. http://dx.doi.org/10.25276/2410-1257-2023-2-66-70.

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The purpose of these case reports is to present a case who developed bilateral serpiginous like choroiditis in association with urogenital infection of Mycobacterium Fortuitum. A 49-year-old female who presented with visual loss was diagnosed with bilateral serpiginous like choroiditis. Uveitis workup findings were negative except for a positive tuberculin skin test positivity. Considering the findings and her residence in an endemic area for m. tuberculosis, empirical treatment for tuberculosis was started. Later in the course of the disease, her urine cytology came back positive for ARB, and

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Franken, Willeke P. J., Joost F. Timmermans, Corine Prins, et al. "Comparison of Mantoux and QuantiFERON TB Gold Tests for Diagnosis of Latent Tuberculosis Infection in Army Personnel." Clinical and Vaccine Immunology 14, no. 4 (2007): 477–80. http://dx.doi.org/10.1128/cvi.00463-06.

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ABSTRACT The tuberculin skin test (TST) was compared with QuantiFERON-TB Gold in-tube (QFT-GIT) test for the diagnosis of tuberculosis in non-Mycobacterium bovis BCG-vaccinated military personnel. Among subjects positive by TST, 44.4% of recruits were positive by QFT-GIT compared with 11.5% subjects tested after missions abroad, suggesting that most TST conversions in the latter group were caused by nontuberculous mycobacteria.

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Tieri, Elga Ersilia, Lucio Marino, Katiuscia Zilli, et al. "Survey of Mycobacterium spp. in Eurasian Badgers (Meles meles) in Central Italy." Animals 14, no. 2 (2024): 219. http://dx.doi.org/10.3390/ani14020219.

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A survey to determine the presence of Mycobacterium spp. in the Abruzzo and Molise regions was conducted by testing samples from 124 badgers found dead or road-killed during the 2013–2021 period. Head lymph nodes were collected from all carcasses, as well as mediastinal lymph nodes from 20 of them, for bacteriological and molecular tests; tissues were inoculated onto a set of solid egg-based Lowenstein–Jensen media and in a liquid culture system (BACTEC) and were analyzed by polymerase chain reactions (PCRs). Organs and lymph nodes from 31 carcasses were collected for histological tests. Durin

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Haanen, J. B., R. de Waal Malefijt, P. C. Res, et al. "Selection of a human T helper type 1-like T cell subset by mycobacteria." Journal of Experimental Medicine 174, no. 3 (1991): 583–92. http://dx.doi.org/10.1084/jem.174.3.583.

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Mycobacteria elicit a cellular immune response in their hosts. This response usually leads to protective immunity, but may sometimes be accompanied by immunopathology due to delayed type hypersensitivity (DTH). A striking example in man is tuberculoid leprosy, which is characterized by high cellular immunity to Mycobacterium leprae and immunopathology due to DTH. Skin lesions of patients suffering from this disease have the characteristics of DTH reactions in which macrophages and CD4+ T lymphocytes predominate. In animal models, it has been shown that DTH responses are associated with the pre

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Gobala Krishnan P, Gnanaprakash K, and Chandrasekhar KB. "Design, synthesis, characterization and antitubercular activity of some nov-el 2, 4-disubstituted thiazole derivatives." International Journal of Research in Pharmaceutical Sciences 10, no. 2 (2019): 1504–9. http://dx.doi.org/10.26452/ijrps.v10i2.729.

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Literature reviews reveal that thiazole and pyrazine carboxamide derivatives exhibit anticonvulsant, antimicrobial, anticancer and anti-tubercular activities due to the presence of –S-C=N- and-CO–NH- moiety. A series of thiazolyl pyrazine carboxamide derivatives (5a-j) were synthesized by condensation reaction between 2-amino, 4-substituted phenyl 2-amino thiazole and pyrazine 2-carboxylic acid. These synthesized thiazole derivatives (5a-j) were evaluated for their inhibitory activity against Mycobacterium tuberculosis (Mtb), H37Rv using microplate Alamar Blue assay (MABA). The compound, 5c an

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Sapriel, Guillaume, and Roland Brosch. "Shared Pathogenomic Patterns Characterize a New Phylotype, Revealing Transition toward Host-Adaptation Long before Speciation of Mycobacterium tuberculosis." Genome Biology and Evolution 11, no. 8 (2019): 2420–38. http://dx.doi.org/10.1093/gbe/evz162.

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Abstract Tuberculosis remains one of the deadliest infectious diseases of humanity. To better understand the evolutionary history of host-adaptation of tubercle bacilli (MTB), we sought for mycobacterial species that were more closely related to MTB than the previously used comparator species Mycobacterium marinum and Mycobacterium kansasii. Our phylogenomic approach revealed some recently sequenced opportunistic mycobacterial pathogens, Mycobacterium decipiens, Mycobacterium lacus, Mycobacterium riyadhense, and Mycobacterium shinjukuense, to constitute a common clade with MTB, hereafter calle

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Lau, S. K., S. Kwan, J. Lee, and W. I. Wei. "Source of tubercle bacilli in cervical lymph nodes: A prospective study." Journal of Laryngology & Otology 105, no. 7 (1991): 558–61. http://dx.doi.org/10.1017/s0022215100116603.

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AbstractA prospective study searching for associated mycobacterial infection of the upper aerodigestive tract in patients with cervical tuberculous lymphadenitis (TBLN) was carried out. One hundred and thirteen patients with a clinical suspicion of cervical TBLN were included. All patients had a physical examination of the upper aerodigestive tract. Routine endoscopy and biopsy of the nasopharynx were performed. All of them had surgery to the cervical lymph node. Seventy-five patients had histologically confirmed cervical TBLN. Culture of the lymph node specimen showed Mycobacterium tuberculos

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Skoric, M., E. J. Shitaye, R. Halouzka, et al. "Tuberculous and tuberculoid lesions in free living small terrestrial mammals and the risk of infection to humans and animals: a review." Veterinární Medicína 52, No. 4 (2008): 144–61. http://dx.doi.org/10.17221/2032-vetmed.

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The present study describes pathogenesis and morphology of tuberculous and tuberculoid lesions in small terrestrial mammals, above all, in rodents. The most serious infectious agents that cause tuberculous and tuberculoid lesions in these animals are also cited. Besides others, the diseases caused by pathogenic mycobacteria that are members of <i>Mycobacterium tuberculosis</i> and <i>M. avium</i> complexes, <i>M. lepraemurium</i>, tularaemia, brucellosis and salmonellosis are included in the present study.

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Zavgorodniy, A. I., S. A. Pozmogova, V. V. Bilushko, et al. "Problems of paraallegic reactions to tuberculin in cattle." Veterinary Medicine: inter-departmental subject scientific collection, no. 110 (October 23, 2024): 139–44. https://doi.org/10.36016/vm-2024-110-21.

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The article presents the results of the analysis of epizootic, clinical, simultaneous allergic study of cattle for tuberculosis and bacteriological examination of samples of biomaterial, feed, scrapings from feeders, and feces. It was found that over four years, 87 cows reacting to tuberculin were detected on the farm, among which no clinically sick cows were found. In four simultaneous allergy tests of cattle, 45 cattle were found to be reactive to tuberculin and an atypical mycobacterial allergen (AAM). In 41 animals, reactions to AAM were more intense compared to reactions to mammalian tube

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Waters, W. R., A. O. Whelan, K. P. Lyashchenko, et al. "Immune Responses in Cattle Inoculated with Mycobacterium bovis, Mycobacterium tuberculosis, or Mycobacterium kansasii." Clinical and Vaccine Immunology 17, no. 2 (2009): 247–52. http://dx.doi.org/10.1128/cvi.00442-09.

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ABSTRACT Cattle were inoculated with Mycobacterium bovis, Mycobacterium tuberculosis, or Mycobacterium kansasii to compare the antigen-specific immune responses to various patterns of mycobacterial disease. Disease expression ranged from colonization with associated pathology (M. bovis infection) and colonization without pathology (M. tuberculosis infection) to no colonization or pathology (M. kansasii infection). Delayed-type hypersensitivity and gamma interferon responses were elicited by each mycobacterial inoculation; however, the responses by the M. bovis- and M. tuberculosis-inoculated a

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Cheon, Seon-Hee, Beate Kampmann, Amy G. Hise, et al. "Bactericidal Activity in Whole Blood as a Potential Surrogate Marker of Immunity after Vaccination against Tuberculosis." Clinical and Vaccine Immunology 9, no. 4 (2002): 901–7. http://dx.doi.org/10.1128/cdli.9.4.901-907.2002.

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ABSTRACT The development of new tuberculosis (TB) vaccines will require the identification of correlates of human protection. This study examined the balance between immunity and virulence in a whole blood infection model in which intracellular mycobacterial survival was measured using BACTEC. In the blood of tuberculin-negative donors, counts of Mycobacterium tuberculosis H37Ra organisms fell by 0.14 log10 CFU during 96 h of whole blood culture, whereas counts of Mycobacterium bovis BCG, M. tuberculosis H37Rv, and a clinical TB isolate's organisms increased by 0.13, 0.43, and 1.04 log10 CFU,

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Toi, Pampa Ch, Apoorvalakshmi Subramaniam, Noyal Mariya Joseph, and Neelaiah Siddaraju. "Role of Immunocytochemistry in Detection of Mycobacterium tuberculosis Antigen by Fine Needle Aspiration Cytology." Journal of Cytology 42, no. 2 (2025): 82–87. https://doi.org/10.4103/joc.joc_37_24.

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Abstract Background: Detection of extrapulmonary tuberculosis (EPTB) by Ziehl-Neelsen (Z-N) staining in fine needle aspirates is challenging due to the low yield of acid-fast bacteria (AFB). Mycobacterial culture, the gold standard, takes 4–8 weeks. Polymerase chain reaction with 100% sensitivity and 92.1% specificity is expensive. Mycobacterial antigens produced by the tubercle bacilli consist of several proteins and enzymes. The protein purified from Mycobacterium tuberculosis is called MPT. The MPT64, a 24-kd protein, has not been detected in non-tuberculous mycobacteria. We aim to study th

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Congia, Stefano, Gianfranco Puddu, Giulio Sorrentino, Giuseppe Dessì, and Giuseppe Marongiu. "Conservative treatment of early-onset tubercular periprosthetic joint infection following total knee arthroplasty." Journal of Infection in Developing Countries 14, no. 02 (2020): 223–27. http://dx.doi.org/10.3855/jidc.12053.

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Tubercular periprosthetic joint infections (PJI) are uncommon diseases in developed countries. Therefore, the systematic screening for Mycobacterium tuberculosis (TB) is not currently recommended before a total knee arthroplasty procedure. However, due to the new human migration flows and higher mycobacterial infection rates, tuberculosis could represent a rare but potential cause for PJI. Controversies about tubercular PJI diagnosis, management and treatment still exist due to a lack of clinical evidence. In the current report we present the case of an early-onset M. tuberculosis PJI of the k

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Maes, Roland. "Mycobacteria’s Antibodies Search: Serology, an Important Neglected Means to Establish a Diagnosis and a Prognosis." Journal of Preventive, Diagnostic and Treatment Strategies in Medicine 2, no. 4 (2023): 202–7. http://dx.doi.org/10.4103/jpdtsm.jpdtsm_124_23.

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The WHO has banned the use of blood tests for the diagnostic and prognostic of tuberculosis. This is a monumental error that it never corrected. The current ban deprives the mycobacterial community of a tool that is essential to combat the disease. Serology estimates the humoral immune status of a patient or a population. It is not a substitute to antigen detection, but it is comparable to the tuberculin test which estimates the cellular immune status. Both give an evocative expression of the tubercle bacillus immunosuppressive activity and indicate the mobilization level of the infected patie

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Sharma, Pawan, Manish Bhargav, and Shivani Saklani. "Tubercular lymphadenopathy." International Journal of Research in Medical Sciences 13, no. 7 (2025): 3048–51. https://doi.org/10.18203/2320-6012.ijrms20252048.

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Tubercular mediastinal lymphadenopathy is a condition characterized by the abnormal enlargement of lymph nodes in the mediastinum also known as tuberculous lymphadenitis which can be acquired from close contact with an infected person. Mycobacterium tuberculli is one of the main causative agents of this dreadful infectious disease. The symptoms usually present are weakness or fatigue, weight loss, chills, fever, and sweating at night. After ruling out the clinical diagnosis it is treated in 2 phases i. e.; intensive phase or the initial phase and continuation phase followed by initial phase. I

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