Academic literature on the topic 'Nucleic-acid Amplification and Quantification'

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Journal articles on the topic "Nucleic-acid Amplification and Quantification"

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Huggett, Jim, Clare Green, and Alimuddin Zumla. "Nucleic acid detection and quantification in the developing world." Biochemical Society Transactions 37, no. 2 (2009): 419–23. http://dx.doi.org/10.1042/bst0370419.

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Techniques using nucleic acid amplification have not had the same amount of impact on research and clinical diagnosis in the developing world as that observed in the West. This is unsurprising when the costs and infrastructure required to perform nucleic acid amplification are considered. Despite this, nucleic acid amplification is being increasingly used in both research and diagnosis in countries such as Zambia and Tanzania. Scientific research in the developing world is made possible through the support and development of the necessary laboratory infrastructure and the establishment of spec
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Kreutz, Jason E., Jiasi Wang, Allison M. Sheen, et al. "Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP." Lab on a Chip 19, no. 6 (2019): 1035–40. http://dx.doi.org/10.1039/c8lc01223g.

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Becherer, Lisa, Jacob Friedrich Hess, Sieghard Frischmann, et al. "Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP." Micromachines 12, no. 2 (2021): 159. http://dx.doi.org/10.3390/mi12020159.

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This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP (mediator displacement loop-mediated isothermal amplification). A disposable microfluidic LabDisk with pre-stored reagents performs automated nucleic acid extraction, reaction setup, emulsification, reverse transcription, digital DNA amplification, and quantitative fluorogenic endpoint detection with universal reporter molecules. Automated nuc
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Fike, Bethany J., Kathrine Curtin, and Peng Li. "Nucleic Acid Target Sensing Using a Vibrating Sharp-Tip Capillary and Digital Droplet Loop-Mediated Isothermal Amplification (ddLAMP)." Sensors 24, no. 13 (2024): 4266. http://dx.doi.org/10.3390/s24134266.

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Nucleic acid tests are key tools for the detection and diagnosis of many diseases. In many cases, the amplification of the nucleic acids is required to reach a detectable level. To make nucleic acid amplification tests more accessible to a point-of-care (POC) setting, isothermal amplification can be performed with a simple heating source. Although these tests are being performed in bulk reactions, the quantification is not as accurate as it would be with digital amplification. Here, we introduce the use of the vibrating sharp-tip capillary for a simple and portable system for tunable on-demand
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Mauk, Michael, Changchun Liu, Jinzhao Song, and Haim Bau. "Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification." Microarrays 4, no. 4 (2015): 474–89. http://dx.doi.org/10.3390/microarrays4040474.

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Gullett, Jonathan C., and Frederick S. Nolte. "Quantitative Nucleic Acid Amplification Methods for Viral Infections." Clinical Chemistry 61, no. 1 (2015): 72–78. http://dx.doi.org/10.1373/clinchem.2014.223289.

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AbstractBACKGROUNDOver the past 2 decades there have been substantial improvements in the methods used to quantify viral nucleic acid in body fluids and in our understanding of how to use viral load measurements in the diagnosis and management of patients with a number of viral infections. These methods are now integrated into a wide range of diagnostic and treatment guidelines and commonly deployed in a variety of clinical settings.CONTENTQuantitative nucleic acid amplification methods that are used to measure viral load are described along with key issues and important variables that affect
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Zhang, Min, Jiajia Wu, Zhaoai Shi, et al. "Molecular Methods for Identification and Quantification of Foodborne Pathogens." Molecules 27, no. 23 (2022): 8262. http://dx.doi.org/10.3390/molecules27238262.

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Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne illness. We summarize simple, rapid, specific, and highly effective molecular technology that is used to detect and identify foodborne pathogens, including polymerase chain reaction, isothermal amplification, loop-mediated isothermal amplification, nucleic acid sequence-based amplification, as well as gene chip and gene probe technology. The principl
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Morcia, Caterina, Roberta Ghizzoni, Chiara Delogu, Lorella Andreani, Paola Carnevali, and Valeria Terzi. "Digital PCR: What Relevance to Plant Studies?" Biology 9, no. 12 (2020): 433. http://dx.doi.org/10.3390/biology9120433.

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Digital PCR (dPCR) is a breakthrough technology that able to provide sensitive and absolute nucleic acid quantification. It is a third-generation technology in the field of nucleic acid amplification. A unique feature of the technique is that of dividing the sample into numerous separate compartments, in each of which an independent amplification reaction takes place. Several instrumental platforms have been developed for this purpose, and different statistical approaches are available for reading the digital output data. The dPCR assays developed so far in the plant science sector were identi
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Kurn, Nurith, Pengchin Chen, Joe Don Heath, Anne Kopf-Sill, Kathryn M. Stephens, and Shenglong Wang. "Novel Isothermal, Linear Nucleic Acid Amplification Systems for Highly Multiplexed Applications." Clinical Chemistry 51, no. 10 (2005): 1973–81. http://dx.doi.org/10.1373/clinchem.2005.053694.

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Abstract Background: Global analysis of the genome, transcriptome, and proteome is facilitated by the recent development of tools for large-scale, highly parallel analysis. We describe a novel nucleic acid amplification system that generates products by several methods. 3′-Ribo-SPIA™ primes cDNA synthesis at the 3′ polyA tail, and whole transcript (WT)-Ribo-SPIA primes cDNA synthesis across the full length of the transcripts and thus provides whole-transcriptome amplification, independent of the 3′ polyA tail. Methods: We developed isothermal linear nucleic acid amplification systems, which us
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Chen, Yanju, Yuanyuan Zhu, Cheng Peng, et al. "A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane." Biosensors 14, no. 3 (2024): 139. http://dx.doi.org/10.3390/bios14030139.

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Nucleic acid quantification, allowing us to accurately know the copy number of target nucleic acids, is significant for diagnosis, food safety, agricultural production, and environmental protection. However, current digital quantification methods require expensive instruments or complicated microfluidic chips, making it difficult to popularize in the point-of-care detection. Paper is an inexpensive and readily available material. In this study, we propose a simple and cost-effective paper membrane-based digital loop-mediated isothermal amplification (LAMP) method for nucleic acid quantificatio
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Dissertations / Theses on the topic "Nucleic-acid Amplification and Quantification"

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Hernández-Neuta, Iván. "Nucleic acid analysis tools : Novel technologies and biomedical applications." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-146334.

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Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. Therefore, accessing these sequences provides key information regarding the molecular identity and functional state of any living organism, this is very useful for areas like biomedicine, where accessing and understanding these molecular signatures is the key to develop strategie
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Lee, Dong-Hun. "Nucleic acid amplification testing for screening of individual blood units." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2007. http://dare.uva.nl/document/48208.

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Daher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.

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Cette thèse de doctorat porte dans l’ensemble une étude approfondie sur une technologie émergente pour l’amplification isotherme des acides nucléiques appelée recombinase polymerase amplification (RPA). L’introduction porte une description détaillée sur la RPA. Cette revue de littérature documente et discute les diverses applications de la RPA en soulignant les connaissances actuelles concernant les applications diagnostiques. Malgré la composition complexe de la RPA (6 à 7 protéines dans le même mélange réactionnel), cette dernière s’avère une technologie rapide (générant des résultats < 2
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Matinyenya, Brian. "Novel and newer nucleic acid amplification tests for the diagnosis of TB." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20680.

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Background: Current tools for TB diagnosis have suboptimal accuracy, perform poorly in diagnosing extra-pulmonary TB, and are not point of care; hence results have a slow turn-around time. Objective: This project evaluated the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the accuracy of Xpert using these fluids was unk
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Syed, Shahida Nina. "Electrochemical control of reversible DNA hybridisation : for future use in nucleic acid amplification." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/9617.

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Denaturation and renaturation is indispensable for the biological function of nucleic acids in many cellular processes, such as for example transcription for the synthesis of RNA and DNA replication during cell division. However, the reversible hybridisation of complementary nucleic acids is equally crucial in nearly all molecular biology technologies, ranging from nucleic acid amplification technologies, such as the polymerase chain reaction, and DNA biosensors to next generation sequencing. For nucleic acid amplification technologies, controlled DNA denaturation and renaturation is particula
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Thomas, Alistair Owen. "Detection of bacterial gene expression by a novel isothermic nucleic acid amplification technology." Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410924.

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A novel probe-based technique called Signal-Mediated Amplification Reaction Technology (SMART) was optimised for detection of RNA targets in order to quantify gene expression. The SMART assay was used to quantify both 23 S rRNA in P. aeruginosa PAOl and gfpmuti mRNA in the plasmid-borne rpoSwgfpmvXi fusions P. aeruginosa SS429 and SS431. However, the assay was not sufficiently sensitive to detect gfpmvfo mRNA from the chromosomal rpoS::gfpmut3 fusion P. aeruginosa SS336. SDS-PAGE analysis of outer membrane proteins of P. aeruginosa PAOl revealed that cells grown in a reported iron-replete chem
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Xiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.

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CORAL, LUCIA. "HIGH-RESOLUTION NUCLEIC ACID ANALYSIS WITH A DNA NANOTECHNOLOGY APPROACH." Doctoral thesis, Università degli Studi di Trieste, 2017. http://hdl.handle.net/11368/2908115.

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The goal of my research program is to develop a DNA-based nanosensor for nucleic acids analysis. I plan to use DNA Origami nanostructures that are formed by a-few-thousand-nucleotides-long, circular, single stranded (ss)DNA “scaffold” folded to form a specific shape by the action of a few hundreds of short (approx. 30 nucleotides) ssDNA “staples”, which hybridize over non-consecutive regions of the scaffold. Staples can be incorporated within the structure with well-defined stoichiometry and some of them can be designed to serve as highly-specific receptor for short nucleic acids sequences. I
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Choi, Kwan-yue. "A molecular epidemiology study on conjunctivitis using conventional nucleic acid amplification technologies and resequencing microarray." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B44248465.

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Choi, Kwan-yue, and 蔡君如. "A molecular epidemiology study on conjunctivitis using conventional nucleic acid amplification technologies and resequencing microarray." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B44248465.

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Books on the topic "Nucleic-acid Amplification and Quantification"

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Myers, Meagan B., and Cynthia A. Schandl, eds. Clinical Applications of Nucleic Acid Amplification. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2950-5.

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A, Toranzos Gary, ed. Environmental applications of nucleic acid amplification techniques. Technomic Pub. Co., 1997.

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Toranzos, Gary A. Environmental Applications of Nucleic Acid Amplification Technology. CRC Press, 2024. http://dx.doi.org/10.1201/9781003578970.

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Lee, Helen H., Stephen A. Morse, and Ørjan Olsvik, eds. Nucleic Acid Amplification Technologies Application to Disease Diagnosis. Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4612-2454-9.

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H, Lee Helen, Morse Stephen A, and Olsvik Ørjan, eds. Nucleic acid amplification technologies: Application to disease diagnosis. Birkhäuser Boston, 1997.

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Zhang, Shusheng, Sai Bi, and Xinyue Song, eds. Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7044-1.

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Lauerman, Lloyd Herman. Nucleic acid amplification assays for diagnosis of animal diseases. American Association of Veterinary Laboratory Diagnosticians, 1998.

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1947-, Kellems Rodney E., ed. Gene amplification in mammalian cells: A comprehensive guide. M. Dekker, 1993.

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Giulio, Pisani, ed. Nucleic acid amplification technology (NAT) for the detection of Hepatitis C Virus (HCV) in plasma pools: Validation report. Istituto superiore di sanità, 2000.

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New York Academy of Sciences. Pharmaceutical science to improve the human condition: Prix Galien 2010 : winners and finalist candidates of the Prix Galien USA, International, and Pro Bono Humanitarian Awards 2010. Wiley Periodicals, 2011.

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Book chapters on the topic "Nucleic-acid Amplification and Quantification"

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Cross, L., C. Potts, and J. G. Anson. "Sensitive and Rapid Detection and Quantification of Nucleic Acids." In Methods in DNA Amplification. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2530-1_3.

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Zhang, Zhenhao, Weimin Tian, Wei Ren, Zhengping Li, and Chenghui Liu. "Quantification of Site-Specific 5-Formylcytosine by Integrating Peptide Nucleic Acid-Clamped Ligation with Loop-Mediated Isothermal Amplification." In Springer Protocols Handbooks. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1229-3_8.

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Yoda, Minami, Jean-Luc Garden, Olivier Bourgeois, et al. "Nucleic Acid Amplification." In Encyclopedia of Nanotechnology. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-90-481-9751-4_100600.

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Li, Richard. "Nucleic Acid Amplification." In Forensic Biology, 3rd ed. CRC Press, 2025. https://doi.org/10.4324/9781003562726-9.

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Shen, Feng. "SlipChip Device for Digital Nucleic Acid DNA nucleic acid amplification Nucleic acid amplification Amplification." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6734-6_10.

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Lehmann, Marc, and Roland P. H. Schmitz. "Nucleic Acid Amplification Techniques." In Modern Techniques for Pathogen Detection. Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527687978.ch3.

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Ørum, Henrik, Christoph Kessler, and Troels Koch. "Peptide Nucleic Acid." In Nucleic Acid Amplification Technologies Application to Disease Diagnosis. Birkhäuser Boston, 1997. http://dx.doi.org/10.1007/978-1-4612-2454-9_3.

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Pannu, Neesh, Xiaoyan Wen, John A. Kellum, et al. "Nucleic Acid Amplification Tests (NAAT)." In Encyclopedia of Intensive Care Medicine. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-00418-6_3224.

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Kessler, Harald H., and Evelyn Stelzl. "Isothermal Nucleic Acid Amplification Methods." In Clinical Virology Manual. ASM Press, 2016. http://dx.doi.org/10.1128/9781555819156.ch12.

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Loens, Katherine, D. Ursi, H. Goossens, and M. Ieven. "Nucleic Acid Sequence-Based Amplification." In Medical Biomethods Handbook. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:273.

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Conference papers on the topic "Nucleic-acid Amplification and Quantification"

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Pardy, Tamás, and Marko Lehes. "Increasing energy-efficiency of microheating in handheld nucleic acid amplification tests." In 2024 19th Biennial Baltic Electronics Conference (BEC). IEEE, 2024. http://dx.doi.org/10.1109/bec61458.2024.10737951.

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Tripathi, Prateek, Nicolas Moser, and Pantelis Georgiou. "In-Pixel Detection of Nucleic Acid Amplification Using Neuromorphic ISFET Arrays." In 2024 IEEE BioSensors Conference (BioSensors). IEEE, 2024. http://dx.doi.org/10.1109/biosensors61405.2024.10712696.

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Gordon, Savannah, Ellie Jaffe, Sebastian Garcia, et al. "Timing Mechanism for a Low-Cost, Single Use, Nucleic Acid Amplification Diagnostic Test." In 2024 IEEE MIT Undergraduate Research Technology Conference (URTC). IEEE, 2024. https://doi.org/10.1109/urtc65039.2024.10937551.

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Sampad, M. J. N., S. M. Saiduzzaman, Z. J. Walker, et al. "Nanopore Integrated Optofluidic Platform for Quantitative Viral RNA Analysis from Clinical Primate Biofluids." In CLEO: Applications and Technology. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.atu3b.4.

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Optical trapping of nucleic acid enriched microbeads enables high-throughput nanopore sensing of molecular biomarkers on optofluidic devices. Amplification-free and label-free quantitative viral RNA analysis, with performances comparable with PCR from clinical animal biofluids, is reported.
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Urban, Nadine, Johanna Groth, Midori Johnston, Hasti Mohsenin, Wilfried Weber, and Can Dincer. "Improvement of the Microfluidic CRISPR/Cas Assay with on-Chip Cleavage for Target-Amplification-Free Nucleic Acid Detection." In 2024 IEEE BioSensors Conference (BioSensors). IEEE, 2024. http://dx.doi.org/10.1109/biosensors61405.2024.10712660.

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Lin, Xueju, Neil Sharma, and Crystal Lee. "Development and Validation of In-Field qPCR Methods for Water Microbial Analysis at Oil and Gas Facilities." In CORROSION 2015. NACE International, 2015. https://doi.org/10.5006/c2015-05948.

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Abstract Quantitative real-time polymerase chain reaction (qPCR) is a highly sensitive method for the quantification of microorganisms in natural and engineered environments such as oil and gas facilities. This study developed in-field methods for sample processing and nucleic acid extraction from water samples, the design and validation of qPCR assays targeting Domains Bacteria and Archaea, and 3 physiological groups (iron-reducing bacteria, sulfate-reducing microorganisms (SRM), and methanogens) of corrosion-causing microorganisms. A novel, field-friendly method was successful for use in iso
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Stanoszek, Lauren, Thomas Blomquist, Erin L. Crawford, et al. "Abstract 2232: Effectiveness evaluation of an in vitro nucleic acid amplification test for quantification of BCR-ABL fusion transcript variants in human whole blood." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2232.

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Weigl, Bernhard H., Gonzalo Domingo, Jay Gerlach, et al. "Non-instrumented nucleic acid amplification assay." In MOEMS-MEMS 2008 Micro and Nanofabrication, edited by Wanjun Wang and Claude Vauchier. SPIE, 2008. http://dx.doi.org/10.1117/12.763650.

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Song, B. F., Q. Song, W. Jin, Q. C. Tian, and Y. Mu. "Absolute Nucleic Acid Quantification System and Analysis." In 2015 International Conference on Industrial Technology and Management Science. Atlantis Press, 2015. http://dx.doi.org/10.2991/itms-15.2015.410.

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Hollenstein, Marcel. "Modified nucleoside triphosphates in rolling circle amplification." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414103.

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Reports on the topic "Nucleic-acid Amplification and Quantification"

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James, Conrad D., Kenneth Roy Pohl, Mark Steven Derzon, Jaime McClain, and Komandoor Achyuthan. Quantification of false positive reduction in nucleic acid purification on hemorrhagic fever DNA. Office of Scientific and Technical Information (OSTI), 2006. http://dx.doi.org/10.2172/899359.

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Shen, Yanqin, Likui Fang, Bo Ye, and Guocan Yu. Diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis: a protocol of systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2020. http://dx.doi.org/10.37766/inplasy2020.6.0030.

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Yu, Guocan, Yanqin Shen, Xudong Xu, and Lihua Lin. Nucleic acid amplification techniques for rapid diagnosis of non-tuberculous mycobacteria: A protocol of systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2020. http://dx.doi.org/10.37766/inplasy2020.11.0076.

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