Academic literature on the topic 'Nucleic acid amplification techniques'

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Journal articles on the topic "Nucleic acid amplification techniques"

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Wolcott, M. J. "Advances in nucleic acid-based detection methods." Clinical Microbiology Reviews 5, no. 4 (1992): 370–86. http://dx.doi.org/10.1128/cmr.5.4.370.

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Laboratory techniques based on nucleic acid methods have increased in popularity over the last decade with clinical microbiologists and other laboratory scientists who are concerned with the diagnosis of infectious agents. This increase in popularity is a result primarily of advances made in nucleic acid amplification and detection techniques. Polymerase chain reaction, the original nucleic acid amplification technique, changed the way many people viewed and used nucleic acid techniques in clinical settings. After the potential of polymerase chain reaction became apparent, other methods of nuc
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Trinh, Thi Ngoc Diep, and Nae Yoon Lee. "Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection." Chemosensors 10, no. 4 (2022): 123. http://dx.doi.org/10.3390/chemosensors10040123.

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Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive. Novel strategies for the identification of pathogenic nucleic acids are necessary for practical application. The advent of microfluidic technology and microfluidic devices has offered advanced and miniaturized tools to rapidly screen microorganisms, improving many drawbacks of conventional nucleic acid amplification-based methods. In this review, we summarize advanc
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O'Leary, J. J., G. Browne, R. Landers, et al. "New nucleic acid amplification techniques in diagnostic pathology." Journal of the European Academy of Dermatology and Venereology 3, no. 1 (1994): 78–86. http://dx.doi.org/10.1111/j.1468-3083.1994.tb00077.x.

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Deng, Huimin, and Zhiqiang Gao. "Bioanalytical applications of isothermal nucleic acid amplification techniques." Analytica Chimica Acta 853 (January 2015): 30–45. http://dx.doi.org/10.1016/j.aca.2014.09.037.

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Antropov, Denis N., and Grigory A. Stepanov. "Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection." Current Issues in Molecular Biology 45, no. 1 (2023): 649–62. http://dx.doi.org/10.3390/cimb45010043.

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Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection. They surpass standard PCR methods in detection time and sensitivity, which is crucial for rapid diagnostics. The first part of this review covers the variety of isothermal amplification methods and describes their reaction mechanisms. Isothermal amplification enables fast multiplication of a target nucleic acid sequence without expensive laboratory equipment. However, researchers ai
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Gorgannezhad, Lena, Helen Stratton, and Nam-Trung Nguyen. "Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology." Micromachines 10, no. 6 (2019): 408. http://dx.doi.org/10.3390/mi10060408.

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Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progre
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Haitao, Qu, Zhang Wenchao, Zhang Xiaohui, Wang Xiujun, and Li Sulong. "Shortening distance of forward and reverse primers for nucleic acid isothermal amplification." Biological Chemistry 395, no. 6 (2014): 679–84. http://dx.doi.org/10.1515/hsz-2014-0103.

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Abstract Existent nucleic acid isothermal detection techniques for clinical diseases are difficult to promote greatly due to limitations in such aspects as methodology, costs of detection, amplification efficiency and conditions for operation. There is therefore an urgent need for a new isothermal amplification method with the characteristics of high accuracy, easy operation, short time of detection and low costs. We have devised a new method of nucleic acid isothermal amplification using Bst DNA polymerase under isothermal conditions (60–65°C). We call this method of amplification by shorteni
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Huggett, Jim, Clare Green, and Alimuddin Zumla. "Nucleic acid detection and quantification in the developing world." Biochemical Society Transactions 37, no. 2 (2009): 419–23. http://dx.doi.org/10.1042/bst0370419.

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Techniques using nucleic acid amplification have not had the same amount of impact on research and clinical diagnosis in the developing world as that observed in the West. This is unsurprising when the costs and infrastructure required to perform nucleic acid amplification are considered. Despite this, nucleic acid amplification is being increasingly used in both research and diagnosis in countries such as Zambia and Tanzania. Scientific research in the developing world is made possible through the support and development of the necessary laboratory infrastructure and the establishment of spec
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Starman, Terri Woods, Xiangrong Duan, and Shane Abbitt. "Nucleic Acid Scanning Techniques Distinguish Closely Related Cultivars of Poinsettia." HortScience 34, no. 6 (1999): 1119–22. http://dx.doi.org/10.21273/hortsci.34.6.1119.

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DNA amplification fingerprinting (DAF) was used to evaluate the genetic relationships among 11 cultivars of poinsettia (Euphorbia pulcherrima Willd.). Amplification was with 10 octamer oligonucleotide primers that generated 336 DNA bands. Thirty-one percent of the bands were polymorphic and distinguished among cultivars. Genetic relationships were evaluated by cluster analysis, and the resulting dendrogram closely agreed with published cultivar relationships. Arbitrary signatures from amplification profiles (ASAP) were further used to characterize two cultivars, `Nutcracker Red' and `Peterstar
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Lee, Soo Min, Hari Kalathil Balakrishnan, Egan H. Doeven, Dan Yuan, and Rosanne M. Guijt. "Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review." Biosensors 13, no. 11 (2023): 980. http://dx.doi.org/10.3390/bios13110980.

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Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological threats, including infectious diseases. Prior to nucleic acid amplification and detection, extensive sample preparation techniques are required to free nucleic acids and extract them from the sample matrix. Sample preparation is critical to maximize the sensitivity and reliabil
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Dissertations / Theses on the topic "Nucleic acid amplification techniques"

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Bowers, Katherine. "Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis." Thesis, University of Westminster, 2017. https://westminsterresearch.westminster.ac.uk/item/q56v6/development-and-clinical-performance-of-nucleic-acid-amplification-techniques-for-the-diagnosis-of-strongyloides-stercoralis.

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The laboratory diagnosis of Strongyloides stercoralis (S. stercoralis) at the Department of Clinical Parasitology (DCP) by the routine methods of microscopy and Strongyloides culture is not sensitive due to the, usually, low parasite load and intermittent larval excretion of the parasite. Serology (enzyme-linked immunosorbent assay) suffers from a lack of specificity because Strongyloides antibodies are known to cross- react with schistosomal, filarial and other helminthic antibodies in serological tests. Moreover, antibody levels are slow to decline after successful treatment therefore serolo
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Bernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.

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Gomez, Deborah Beltrami. "Prevalência de Chlamydia trachomatis em mulheres inférteis e gestantes assintomáticas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143385.

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Introdução: A infecção urogenital por Chlamydia trachomatis é a doença sexualmente transmissível bacteriana mais prevalente no mundo e afeta principalmente mulheres jovens sexualmente ativas. Infecções não tratadas podem provocar complicações reprodutivas decorrentes do dano tubáreo. Na gestação, aumenta o risco de parto prematuro, baixo peso ao nascer, morte perinatal, conjuntivite e pneumonia neonatal. Existem poucos dados brasileiros referentes à epidemiologia dessa infecção no nosso meio. O objetivo desse estudo foi determinar a prevalência de C. trachomatis em mulheres inférteis e em gest
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Lee, Dong-Hun. "Nucleic acid amplification testing for screening of individual blood units." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2007. http://dare.uva.nl/document/48208.

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Daher, Rana. "Recombinase polymerase amplification technology : Assessment for nucleic acid-based acid-based point-of-care diagnostics." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26269.

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Cette thèse de doctorat porte dans l’ensemble une étude approfondie sur une technologie émergente pour l’amplification isotherme des acides nucléiques appelée recombinase polymerase amplification (RPA). L’introduction porte une description détaillée sur la RPA. Cette revue de littérature documente et discute les diverses applications de la RPA en soulignant les connaissances actuelles concernant les applications diagnostiques. Malgré la composition complexe de la RPA (6 à 7 protéines dans le même mélange réactionnel), cette dernière s’avère une technologie rapide (générant des résultats < 2
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Matinyenya, Brian. "Novel and newer nucleic acid amplification tests for the diagnosis of TB." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20680.

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Background: Current tools for TB diagnosis have suboptimal accuracy, perform poorly in diagnosing extra-pulmonary TB, and are not point of care; hence results have a slow turn-around time. Objective: This project evaluated the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the accuracy of Xpert using these fluids was unk
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Syed, Shahida Nina. "Electrochemical control of reversible DNA hybridisation : for future use in nucleic acid amplification." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/9617.

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Denaturation and renaturation is indispensable for the biological function of nucleic acids in many cellular processes, such as for example transcription for the synthesis of RNA and DNA replication during cell division. However, the reversible hybridisation of complementary nucleic acids is equally crucial in nearly all molecular biology technologies, ranging from nucleic acid amplification technologies, such as the polymerase chain reaction, and DNA biosensors to next generation sequencing. For nucleic acid amplification technologies, controlled DNA denaturation and renaturation is particula
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Thomas, Alistair Owen. "Detection of bacterial gene expression by a novel isothermic nucleic acid amplification technology." Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410924.

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A novel probe-based technique called Signal-Mediated Amplification Reaction Technology (SMART) was optimised for detection of RNA targets in order to quantify gene expression. The SMART assay was used to quantify both 23 S rRNA in P. aeruginosa PAOl and gfpmuti mRNA in the plasmid-borne rpoSwgfpmvXi fusions P. aeruginosa SS429 and SS431. However, the assay was not sufficiently sensitive to detect gfpmvfo mRNA from the chromosomal rpoS::gfpmut3 fusion P. aeruginosa SS336. SDS-PAGE analysis of outer membrane proteins of P. aeruginosa PAOl revealed that cells grown in a reported iron-replete chem
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Xiao, Linlin. "Detection of Viable Foodborne Pathogens and Spoilage Microorganisms by Nucleic Acid Amplification Based Platforms." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308284180.

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CORAL, LUCIA. "HIGH-RESOLUTION NUCLEIC ACID ANALYSIS WITH A DNA NANOTECHNOLOGY APPROACH." Doctoral thesis, Università degli Studi di Trieste, 2017. http://hdl.handle.net/11368/2908115.

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The goal of my research program is to develop a DNA-based nanosensor for nucleic acids analysis. I plan to use DNA Origami nanostructures that are formed by a-few-thousand-nucleotides-long, circular, single stranded (ss)DNA “scaffold” folded to form a specific shape by the action of a few hundreds of short (approx. 30 nucleotides) ssDNA “staples”, which hybridize over non-consecutive regions of the scaffold. Staples can be incorporated within the structure with well-defined stoichiometry and some of them can be designed to serve as highly-specific receptor for short nucleic acids sequences. I
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Books on the topic "Nucleic acid amplification techniques"

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A, Toranzos Gary, ed. Environmental applications of nucleic acid amplification techniques. Technomic Pub. Co., 1997.

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1947-, Kellems Rodney E., ed. Gene amplification in mammalian cells: A comprehensive guide. M. Dekker, 1993.

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New York Academy of Sciences. Pharmaceutical science to improve the human condition: Prix Galien 2010 : winners and finalist candidates of the Prix Galien USA, International, and Pro Bono Humanitarian Awards 2010. Wiley Periodicals, 2011.

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Myers, Meagan B., and Cynthia A. Schandl, eds. Clinical Applications of Nucleic Acid Amplification. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2950-5.

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Walker, John M. New Nucleic Acid Techniques. Humana Press, 1988. http://dx.doi.org/10.1385/0896031276.

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1948-, Walker John M., ed. New nucleic acid techniques. Humana Press, 1988.

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Bernd, Kochanowski, and Reischl Udo, eds. Quantitative PCR protocols. Humana Press, 1999.

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Lee, Helen H., Stephen A. Morse, and Ørjan Olsvik, eds. Nucleic Acid Amplification Technologies Application to Disease Diagnosis. Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4612-2454-9.

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H, Lee Helen, Morse Stephen A, and Olsvik Ørjan, eds. Nucleic acid amplification technologies: Application to disease diagnosis. Birkhäuser Boston, 1997.

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Zhang, Shusheng, Sai Bi, and Xinyue Song, eds. Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7044-1.

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Book chapters on the topic "Nucleic acid amplification techniques"

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Lehmann, Marc, and Roland P. H. Schmitz. "Nucleic Acid Amplification Techniques." In Modern Techniques for Pathogen Detection. Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527687978.ch3.

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Towner, K. J., and A. Cockayne. "Nucleic acid amplification and sequencing techniques." In Molecular Methods for Microbial Identification and Typing. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1506-3_4.

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Li, Haijing, and Yi-Wei Tang. "An Introduction to In Vitro Nucleic Acid Amplification Techniques." In Advanced Techniques in Diagnostic Microbiology. Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-3970-7_14.

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Sundsfjord, Arnfinn, and Ørjan Olsvik. "Nucleic Acid Amplification Techniques in Detection and Diagnosis of Medically Important Viral Infections." In Nucleic Acid Amplification Technologies Application to Disease Diagnosis. Birkhäuser Boston, 1997. http://dx.doi.org/10.1007/978-1-4612-2454-9_14.

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Song, Xinyue, and Yao Jiang. "Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application." In Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7044-1_2.

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Burghoff, Robert L., Jens Beator, and Michael A. Harvey. "Psoralen Biotin: A Novel Reagent for Non-Enzymatic and Specific Labeling of Nucleic Acid Probes and Oligonucleotides." In Modern Applications of DNA Amplification Techniques. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5379-3_8.

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Milton, A. Arun Prince, G. Bhuvana Priya, Sandeep Ghatak, Samir Das, and M. Chendu Bharat Prasad. "Isothermal Nucleic Acid Amplification Techniques: New Horizons in Microbial Food Safety Applications." In Bioresources and Bioprocess in Biotechnology for a Sustainable Future. Apple Academic Press, 2024. http://dx.doi.org/10.1201/9781003410041-14.

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Pandrangi, Santhilatha, G. Kishore, Gantala Sarva Sai Nikhilesh, and Suseela Lanka. "Nucleic Acid Amplification and Molecular Diagnostic Techniques in the Detection of ESKAPE Bacterial Pathogens." In ESKAPE Pathogens. Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-99-8799-3_5.

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Czurda, Stefan, and Thomas Lion. "Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6515-1_14.

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Yoda, Minami, Jean-Luc Garden, Olivier Bourgeois, et al. "Nucleic Acid Amplification." In Encyclopedia of Nanotechnology. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-90-481-9751-4_100600.

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Conference papers on the topic "Nucleic acid amplification techniques"

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Kopacz, Adrian M., Wing K. Liu, and Jae-Hyun Chung. "Design and Optimization of a Nanotip Sensor via Immersed Molecular Electrokinetic Finite Element Method." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13299.

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A critical challenge in the field of medicine is to develop a low cost sensor competent of detecting specific bacterial pathogens via a precise deoxyribonucleic acid (DNA) sequence. In order to identify such biological agents in a patient’s blood or other bodily fluids at the onset of infection, detection of specific pathogen genomic DNA is considered a reliable approach. Current techniques involving multiplex DNA/RNA detection arrays or immunoassays [1] require cumbersome sample preparation, aggressive nucleic acid amplification protocols and must be operated by trained personnel. To overcome
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Jaunarena, Ibon, Marta Rezola, Maria Carrillo, et al. "#608 Expression of cytokeratin 19 in vulvar carcinomas for sentinel lymph node biopsy assessment with one-step nucleic acid amplification technique." In ESGO 2023 Congress. BMJ Publishing Group Ltd, 2023. http://dx.doi.org/10.1136/ijgc-2023-esgo.825.

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Jaunarena, Ibon, Marta Rezola, Ainhoa Ordoñez, et al. "1089 Expression of cytokeratin 19 in vulvar carcinomas for sentinel lymph node biopsy assessment with one-step nucleic acid amplification technique." In ESGO 2024 Congress Abstracts. BMJ Publishing Group Ltd, 2024. http://dx.doi.org/10.1136/ijgc-2024-esgo.1062.

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Weigl, Bernhard H., Gonzalo Domingo, Jay Gerlach, et al. "Non-instrumented nucleic acid amplification assay." In MOEMS-MEMS 2008 Micro and Nanofabrication, edited by Wanjun Wang and Claude Vauchier. SPIE, 2008. http://dx.doi.org/10.1117/12.763650.

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Hollenstein, Marcel. "Modified nucleoside triphosphates in rolling circle amplification." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414103.

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Ménová, Petra, and Michal Hocek. "Preparation of modified oligonucleotides by nicking enzyme amplification reaction." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414324.

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Liu, Mingdian, Zheyan Tang, Hosein Monshat, Yuxin Zhao, and Meng Lu. "Portable instrument for paper-based isothermal nucleic acid amplification tests." In CLEO: Applications and Technology. OSA, 2020. http://dx.doi.org/10.1364/cleo_at.2020.jtu2f.13.

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LaBarre, Paul, David Boyle, Kenneth Hawkins, and Bernhard Weigl. "Instrument-free nucleic acid amplification assays for global health settings." In SPIE Defense, Security, and Sensing, edited by Sárka O. Southern, Kevin N. Montgomery, Carl W. Taylor, et al. SPIE, 2011. http://dx.doi.org/10.1117/12.882868.

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Meagher, Robert. "LAMP & Isothermal Nucleic Acid Amplification: Ready for Prime Time?." In Proposed for presentation at the Molecular & Precision Med Tri-Con held February 21-23, 2022 in San Diego, CA. US DOE, 2022. http://dx.doi.org/10.2172/2001688.

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Efimov, V. A., O. G. Chakhmakhcheva, M. V. Choob, and D. Archdeacon. "Using chimeric DNA/RNA molecular beacons for target-specific signal amplification." In XIIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2002. http://dx.doi.org/10.1135/css200205308.

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Reports on the topic "Nucleic acid amplification techniques"

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Yu, Guocan, Yanqin Shen, Xudong Xu, and Lihua Lin. Nucleic acid amplification techniques for rapid diagnosis of non-tuberculous mycobacteria: A protocol of systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2020. http://dx.doi.org/10.37766/inplasy2020.11.0076.

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Trewhella, J., E. M. Bradbury, G. Gupta, B. Imai, R. Martinez, and C. Unkefer. Development of experimental techniques to study protein and nucleic acid structures. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/212551.

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Shen, Yanqin, Likui Fang, Bo Ye, and Guocan Yu. Diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis: a protocol of systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2020. http://dx.doi.org/10.37766/inplasy2020.6.0030.

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