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Journal articles on the topic 'Nucleic acid amplification techniques'

Author: Grafiati

Published: 4 June 2021

Last updated: 29 July 2024

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1

Wolcott, M. J. "Advances in nucleic acid-based detection methods." Clinical Microbiology Reviews 5, no. 4 (1992): 370–86. http://dx.doi.org/10.1128/cmr.5.4.370.

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Laboratory techniques based on nucleic acid methods have increased in popularity over the last decade with clinical microbiologists and other laboratory scientists who are concerned with the diagnosis of infectious agents. This increase in popularity is a result primarily of advances made in nucleic acid amplification and detection techniques. Polymerase chain reaction, the original nucleic acid amplification technique, changed the way many people viewed and used nucleic acid techniques in clinical settings. After the potential of polymerase chain reaction became apparent, other methods of nuc

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2

Trinh, Thi Ngoc Diep, and Nae Yoon Lee. "Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection." Chemosensors 10, no. 4 (2022): 123. http://dx.doi.org/10.3390/chemosensors10040123.

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Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive. Novel strategies for the identification of pathogenic nucleic acids are necessary for practical application. The advent of microfluidic technology and microfluidic devices has offered advanced and miniaturized tools to rapidly screen microorganisms, improving many drawbacks of conventional nucleic acid amplification-based methods. In this review, we summarize advanc

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3

O'Leary, J. J., G. Browne, R. Landers, et al. "New nucleic acid amplification techniques in diagnostic pathology." Journal of the European Academy of Dermatology and Venereology 3, no. 1 (1994): 78–86. http://dx.doi.org/10.1111/j.1468-3083.1994.tb00077.x.

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4

Deng, Huimin, and Zhiqiang Gao. "Bioanalytical applications of isothermal nucleic acid amplification techniques." Analytica Chimica Acta 853 (January 2015): 30–45. http://dx.doi.org/10.1016/j.aca.2014.09.037.

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5

Antropov, Denis N., and Grigory A. Stepanov. "Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection." Current Issues in Molecular Biology 45, no. 1 (2023): 649–62. http://dx.doi.org/10.3390/cimb45010043.

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Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection. They surpass standard PCR methods in detection time and sensitivity, which is crucial for rapid diagnostics. The first part of this review covers the variety of isothermal amplification methods and describes their reaction mechanisms. Isothermal amplification enables fast multiplication of a target nucleic acid sequence without expensive laboratory equipment. However, researchers ai

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6

Gorgannezhad, Lena, Helen Stratton, and Nam-Trung Nguyen. "Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology." Micromachines 10, no. 6 (2019): 408. http://dx.doi.org/10.3390/mi10060408.

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Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progre

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Haitao, Qu, Zhang Wenchao, Zhang Xiaohui, Wang Xiujun, and Li Sulong. "Shortening distance of forward and reverse primers for nucleic acid isothermal amplification." Biological Chemistry 395, no. 6 (2014): 679–84. http://dx.doi.org/10.1515/hsz-2014-0103.

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Abstract Existent nucleic acid isothermal detection techniques for clinical diseases are difficult to promote greatly due to limitations in such aspects as methodology, costs of detection, amplification efficiency and conditions for operation. There is therefore an urgent need for a new isothermal amplification method with the characteristics of high accuracy, easy operation, short time of detection and low costs. We have devised a new method of nucleic acid isothermal amplification using Bst DNA polymerase under isothermal conditions (60–65°C). We call this method of amplification by shorteni

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8

Huggett, Jim, Clare Green, and Alimuddin Zumla. "Nucleic acid detection and quantification in the developing world." Biochemical Society Transactions 37, no. 2 (2009): 419–23. http://dx.doi.org/10.1042/bst0370419.

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Techniques using nucleic acid amplification have not had the same amount of impact on research and clinical diagnosis in the developing world as that observed in the West. This is unsurprising when the costs and infrastructure required to perform nucleic acid amplification are considered. Despite this, nucleic acid amplification is being increasingly used in both research and diagnosis in countries such as Zambia and Tanzania. Scientific research in the developing world is made possible through the support and development of the necessary laboratory infrastructure and the establishment of spec

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9

Starman, Terri Woods, Xiangrong Duan, and Shane Abbitt. "Nucleic Acid Scanning Techniques Distinguish Closely Related Cultivars of Poinsettia." HortScience 34, no. 6 (1999): 1119–22. http://dx.doi.org/10.21273/hortsci.34.6.1119.

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DNA amplification fingerprinting (DAF) was used to evaluate the genetic relationships among 11 cultivars of poinsettia (Euphorbia pulcherrima Willd.). Amplification was with 10 octamer oligonucleotide primers that generated 336 DNA bands. Thirty-one percent of the bands were polymorphic and distinguished among cultivars. Genetic relationships were evaluated by cluster analysis, and the resulting dendrogram closely agreed with published cultivar relationships. Arbitrary signatures from amplification profiles (ASAP) were further used to characterize two cultivars, `Nutcracker Red' and `Peterstar

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10

Lee, Soo Min, Hari Kalathil Balakrishnan, Egan H. Doeven, Dan Yuan, and Rosanne M. Guijt. "Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review." Biosensors 13, no. 11 (2023): 980. http://dx.doi.org/10.3390/bios13110980.

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Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological threats, including infectious diseases. Prior to nucleic acid amplification and detection, extensive sample preparation techniques are required to free nucleic acids and extract them from the sample matrix. Sample preparation is critical to maximize the sensitivity and reliabil

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11

Zhu, Xiaoli, Chang Feng, Bin Zhang, Hui Tong, Tao Gao, and Genxi Li. "A netlike rolling circle nucleic acid amplification technique." Analyst 140, no. 1 (2015): 74–78. http://dx.doi.org/10.1039/c4an01711k.

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An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.

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12

Ouyang, Wei, and Jongyoon Han. "Universal amplification-free molecular diagnostics by billion-fold hierarchical nanofluidic concentration." Proceedings of the National Academy of Sciences 116, no. 33 (2019): 16240–49. http://dx.doi.org/10.1073/pnas.1904513116.

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Rapid and reliable detection of ultralow-abundance nucleic acids and proteins in complex biological media may greatly advance clinical diagnostics and biotechnology development. Currently, nucleic acid tests rely on enzymatic processes for target amplification (e.g., PCR), which have many inherent issues restricting their implementation in diagnostics. On the other hand, there exist no protein amplification techniques, greatly limiting the development of protein-based diagnosis. We report a universal biomolecule enrichment technique termed hierarchical nanofluidic molecular enrichment system (

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13

GRAY, J., and L. J. COUPLAND. "The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections." Epidemiology and Infection 142, no. 1 (2013): 1–11. http://dx.doi.org/10.1017/s0950268813002367.

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SUMMARYOn 14 January 2013, the US Food and Drug Administration (FDA) announced permission for a multiplex nucleic acid test, the xTAG®Gastrointestinal Pathogen Panel (GPP) (Luminex Corporation, USA), which simultaneously detects 11 common viral, bacterial and parasitic causes of infectious gastroenteritis, to be marketed in the USA. This announcement reflects the current move towards the development and commercialization of detection technologies based on nucleic acid amplification techniques for diagnosis of syndromic infections. We discuss the limitations and advantages of nucleic acid ampli

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14

Nübling, C. M., R. Seitz, and J. Löwer. "Application of Nucleic Acid Amplification Techniques for Blood Donation Screening." Transfusion Medicine and Hemotherapy 25, no. 2-3 (1998): 86–90. http://dx.doi.org/10.1159/000053399.

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15

Monis, Paul T., and Steven Giglio. "Nucleic acid amplification-based techniques for pathogen detection and identification." Infection, Genetics and Evolution 6, no. 1 (2006): 2–12. http://dx.doi.org/10.1016/j.meegid.2005.08.004.

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Bodulev, O. L., and I. Yu Sakharov. "Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis." Biochemistry (Moscow) 85, no. 2 (2020): 147–66. http://dx.doi.org/10.1134/s0006297920020030.

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Chen, Mengtao, Xinyue Lan, Longjiao Zhu, Ping Ru, Haiyan Liu, and Wentao Xu. "Nucleic acid-aided molecular amplification techniques for food microorganism detection." TrAC Trends in Analytical Chemistry 165 (August 2023): 117116. http://dx.doi.org/10.1016/j.trac.2023.117116.

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18

Cartwright, Charles P. "Techniques and diagnostic applications of in vitro nucleic-acid amplification." Clinical Microbiology Newsletter 16, no. 5 (1994): 33–37. http://dx.doi.org/10.1016/0196-4399(94)90039-6.

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19

Buddhachat, Kittisak. "LAMP assay is an auxiliary tool for rapid and sensitive pathogen diagnosis in veterinary areas." Veterinary Integrative Sciences 20, no. 2 (2020): 247–51. http://dx.doi.org/10.12982/vis.2022.019.

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Molecular technique for nucleic acid amplification has become more advanced, especially isothermal nucleic acid amplification which can amplify DNA or RNA molecules at constant temperatures. This allows us to be independent of sophisticated equipment such as thermal cyclers or realtime-PCR. The feasibility of adopting molecular techniques for pathogen detection at point-of-care are approaching practical use. At present, there are various isothermal nucleic acid amplification technologies, including rolling cycle amplification (RCA), helicase-dependent amplification (HDA), recombinase polymeras

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20

Lan, Lin, Jin Huang, Mengtan Liu, et al. "Polymerization and isomerization cyclic amplification for nucleic acid detection with attomolar sensitivity." Chemical Science 12, no. 12 (2021): 4509–18. http://dx.doi.org/10.1039/d0sc05457g.

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DNA amplification is one of the most valuable tools for the clinical diagnosis of nucleic acid-related diseases, but current techniques for DNA amplification are based on intermolecular polymerization reactions, resulting in the risk of errors in the intermolecular reaction pattern.

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21

Shipitsyna, Е. V., О. V. Budilovskaya, and А. М. Savitcheva. "Nucleic acid sequence—based amplification (nasba) and its application in obstetrical and gynecological practice." Journal of obstetrics and women's diseases 54, no. 2 (2005): 83–89. http://dx.doi.org/10.17816/jowd82490.

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Method of isothermal amplification of nucleic acids NASBA {Nucleic Acid SequenceBased Amplification) is becoming widely used in diagnostic molecular microbiology including diagnostics of infections in pregnant women and newborn infants. NASBA method possesses the unique ability to amplify RNA target selectively in the presence of DNA target of identical sequence, which determines its main application areas: diagnosis of RNA viruses, investigation of bacterial and viral gene expression, diagnosis of bacterial infections based on 16S rRNA detection. In the article the principle and the main step

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22

Wang, Jing, Dong-Xia Wang, Jia-Yi Ma, Ya-Xin Wang, and De-Ming Kong. "Three-dimensional DNA nanostructures to improve the hyperbranched hybridization chain reaction." Chemical Science 10, no. 42 (2019): 9758–67. http://dx.doi.org/10.1039/c9sc02281c.

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23

Iwe, Idorenyin A., Wenqin Li, Zhigang Li, and Jiahao Huang. "Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing." Talanta 226 (May 2021): 122146. http://dx.doi.org/10.1016/j.talanta.2021.122146.

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24

Dassanayake, R. S., and L. P. Samaranayake. "Amplification-Based Nucleic Acid Scanning Techniques to Assess Genetic Polymorphism inCandida." Critical Reviews in Microbiology 29, no. 1 (2003): 1–24. http://dx.doi.org/10.1080/713610402.

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25

DING, Xiong, and Ying MU. "Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques." Chinese Journal of Analytical Chemistry 44, no. 4 (2016): 512–21. http://dx.doi.org/10.1016/s1872-2040(16)60918-0.

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26

Tan, Lin, Liu Xu, Jin-Wen Liu, Li-Juan Tang, Hao Tang, and Ruqin Yu. "Duplex-specific nuclease-mediated target recycling amplification for fluorescence detection of microRNA." Analytical Methods 11, no. 2 (2019): 200–204. http://dx.doi.org/10.1039/c8ay02265h.

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27

Frigo, N. V., S. A. Polevshchikova, I. A. Volkov, et al. "Current methods to identify the gonococcal infection pathogen." Vestnik dermatologii i venerologii 87, no. 3 (2011): 45–51. http://dx.doi.org/10.25208/vdv1017.

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The authors present the data on the current laboratory methods and recommendations for identifying the gonorrhea pathogen applied in Russia and abroad: microscopy method, cultural (bacteriological) study method, nucleic acid amplification techniques and DNA chip technology. The benefits and shortcomings of each of the techniques have been compared. The authors emphasize the cultural method is currently considered to be the key one for diagnosing gonorrhea due to its high specificity and sensitivity making it possible to define the N.gonorrhoeae sensitivity to antimicrobial drugs, which is very

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28

Ngoc, Le Thi Nhu, and Young-Chul Lee. "Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms." Biosensors 14, no. 2 (2024): 97. http://dx.doi.org/10.3390/bios14020097.

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Ribonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some limitations. It is expensive and time-consuming, with infrastructure and trained personnel requirements. Its high throughput requires sophisticated automation and large-scale infrastructure. Isothermal amplification methods have been explored as an alternative to address these challenges. These methods are rapid, user-friendly, low-cost, can be perfor

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Ieven, M., and H. Goossens. "Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory." Clinical Microbiology Reviews 10, no. 2 (1997): 242–56. http://dx.doi.org/10.1128/cmr.10.2.242.

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Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. In general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existing tests (e.g., viral culture) are much longer than those expected with amplification. This is the ca

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Wu, Weidong, Manish Biyani, Daisuke Hirose, and Yuzuru Takamura. "Rapid and Highly Sensitive Detection of Leishmania by Combining Recombinase Polymerase Amplification and Solution-Processed Oxide Thin-Film Transistor Technology." Biosensors 13, no. 8 (2023): 765. http://dx.doi.org/10.3390/bios13080765.

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Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming. Thus, this study aims to combine recombinase polymerase amplification (RPA), which enables the rapid amplification of even trace amounts of nucleic acid fragments within 10–40 min at 37–42 °C, and solution-processed oxide thin-film transistor (TFT) technology, which exhibits high detection sensitivity, to detect Leishmania. A single-stranded anti-probe was incorporated into the RPA primer to facilitate effective hybridization between the R

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Shin, Dong Jin, Nadya Andini, Kuangwen Hsieh, Samuel Yang, and Tza-Huei Wang. "Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing." Annual Review of Analytical Chemistry 12, no. 1 (2019): 41–67. http://dx.doi.org/10.1146/annurev-anchem-061318-115529.

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In the face of looming threats from multi-drug resistant microorganisms, there is a growing need for technologies that will enable rapid identification and drug susceptibility profiling of these pathogens in health care settings. In particular, recent progress in microfluidics and nucleic acid amplification is pushing the boundaries of timescale for diagnosing bacterial infections. With a diverse range of techniques and parallel developments in the field of analytical chemistry, an integrative perspective is needed to understand the significance of these developments. This review examines the

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Sang, Panting, Zhigang Hu, Yuliang Cheng, et al. "Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance." Journal of Agricultural and Food Chemistry 69, no. 21 (2021): 5783–97. http://dx.doi.org/10.1021/acs.jafc.0c07980.

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Kwon, Sangha, and Ha Youn Shin. "Advanced CRISPR-Cas Effector Enzyme-Based Diagnostics for Infectious Diseases, Including COVID-19." Life 11, no. 12 (2021): 1356. http://dx.doi.org/10.3390/life11121356.

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Rapid and precise diagnostic tests can prevent the spread of diseases, including worldwide pandemics. Current commonly used diagnostic methods include nucleic-acid-amplification-based detection methods and immunoassays. These techniques, however, have several drawbacks in diagnosis time, accuracy, and cost. Nucleic acid amplification methods are sensitive but time-consuming, whereas immunoassays are more rapid but relatively insensitive. Recently developed CRISPR-based nucleic acid detection methods have been found to compensate for these limitations. In particular, the unique collateral enzym

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34

Yoo, Hee-Min, Il-Hwan Kim, and Seil Kim. "Nucleic Acid Testing of SARS-CoV-2." International Journal of Molecular Sciences 22, no. 11 (2021): 6150. http://dx.doi.org/10.3390/ijms22116150.

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The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse

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35

Chauhan, Khushbu, Jin Woo, Woong Jung, and Dong-Eun Kim. "Poly(ethylene glycol)-Engrafted Graphene Oxide for Gene Delivery and Nucleic Acid Amplification." Materials 16, no. 23 (2023): 7434. http://dx.doi.org/10.3390/ma16237434.

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Graphene oxide (GO) is an oxidized form of graphene accommodating various oxygen-containing functional groups such as hydroxyl, epoxy, and carboxyl groups on its surface. GO has been extensively utilized in various biomedical applications including the delivery of biomolecules and the development of biosensors owing to its beneficial properties such as high surface area, nucleic acid adsorption, and fluorescence quenching through fluorescence resonance energy transfer (FRET). However, despite these favorable properties, the direct utilization of GO in these applications is often limited by low

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Mumtaz, Zilwa, Zubia Rashid, Ashaq Ali, et al. "Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches." Biosensors 13, no. 6 (2023): 584. http://dx.doi.org/10.3390/bios13060584.

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Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices. Due to the expeditious nature of microfluidic platforms, the trend has been shifted toward the implementation of these systems for the detection of analytes in biochemical, clinical and food technology. Microfluidic systems molded with substances such as polymers or glass offer the specific and sensitive detection of infectious and noninfectious disease

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37

Hubbard, Roger A. "Human Papillomavirus Testing Methods." Archives of Pathology & Laboratory Medicine 127, no. 8 (2003): 940–45. http://dx.doi.org/10.5858/2003-127-940-hptm.

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Abstract Testing for human papillomavirus (HPV) relies exclusively on techniques of molecular biology using nucleic acid probes. Tests for HPV using nucleic acid probes have been commercially available since the late 1980s, but early tests were cumbersome, involving the use of nucleic acid probes labeled with radioactive phosphorus (32P). These early HPV tests did not achieve widespread use because they did not detect all oncogenic HPV genotypes. The current commercial HPV detection kit, Digene's Hybrid Capture 2 kit, detects virtually all high-risk oncogenic HPV types, as well as most low-ris

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Subramonian, Subiksha, Sharat Chopra, and Raghavan Vidya. "New Alternative Techniques for Sentinel Lymph Node Biopsy." Medicina 59, no. 12 (2023): 2077. http://dx.doi.org/10.3390/medicina59122077.

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Background and Objectives: This review paper highlights the key alternatives to the blue dye/radioisotope method of sentinel lymph node biopsy (SLNB). It analyses the research available on these alternative methods and their outcomes compared to the traditional techniques. Materials and Methods: This review focused on fifteen articles, of which five used indocyanine green (ICG) as a tracer, four used magnetic tracers, one used one-step nucleic acid amplification (OSNA) and Metasin (quantitative reverse transcriptase-polymerase chain reaction), one used the photosensitiser talaporfin sodium, on

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Cadena-Ullauri, Santiago, Anibal Gaviria, Patricia Guevara-Ramirez, et al. "Nucleic acid amplification testing (NAT) impact on blood safety compared to Immunoassays in blood banks: A Review." Bionatura 8, no. 4 (2023): 1–15. http://dx.doi.org/10.21931/rb/2023.08.04.33.

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Blood transfusions are fundamental to clinical procedures; however, many people worldwide cannot access safe blood. Blood product safety must be essential in each country’s national health policies. Several aspects of the blood donation process are carefully performed, including laboratory testing comprising blood type determination, antigen-antibody analyses, and nucleic acid amplification testing (NAT); however, NAT is not mandatory in all countries. The traditional screening method is based on antigen-antibody binding techniques, such as ELISA (enzyme-linked immunosorbent assay), with high

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Kaptilnyy, Valery A. "Sample Biological Material Operating Procedure for the method of molecular-biological techniques (PCR diagnostics)." V.F.Snegirev Archives of Obstetrics and Gynecology 3, no. 3 (2016): 156–60. http://dx.doi.org/10.18821/2313-8726-2016-3-3-156-160.

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Review describes a method of diagnostics with the use of the polymerase chain reaction (PCR diagnostics) of infections in patients in modern obstetric-gynecological practice. Particular attention is paid to urogenital tract pathogens to be diagnosed by the method of nucleic acid amplification. There are described the preparation for the study, purposes, the technology of amplification procedure. There is shown the difference in the detection of the DNA and RNA pathogen. The presented photographs show in detail all the stages of obtaining scrapings from the urogenital tract.

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Nocker, Andreas, and Anne K. Camper. "Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques." FEMS Microbiology Letters 291, no. 2 (2009): 137–42. http://dx.doi.org/10.1111/j.1574-6968.2008.01429.x.

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Birch, L., C. E. Dawson, J. H. Cornett, and J. T. Keer. "A comparison of nucleic acid amplification techniques for the assessment of bacterial viability." Letters in Applied Microbiology 33, no. 4 (2001): 296–301. http://dx.doi.org/10.1046/j.1472-765x.2001.00999.x.

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43

Harding, Martha J., Isabelle Prud'homme, and Jerzy Rola. "Identification of the major North American bluetongue viruses using nucleic acid amplification techniques." Molecular and Cellular Probes 9, no. 4 (1995): 223–31. http://dx.doi.org/10.1016/s0890-8508(95)90081-0.

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44

Hughes, Ralph, David Wonderling, Bernadette Li, and Bernard Higgins. "The cost effectiveness of Nucleic Acid Amplification Techniques for the diagnosis of tuberculosis." Respiratory Medicine 106, no. 2 (2012): 300–307. http://dx.doi.org/10.1016/j.rmed.2011.10.005.

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Jiang, Hao, Yuan Li, Xuefei Lv, Yulin Deng, and Xiaoqiong Li. "Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection." Talanta 260 (August 2023): 124645. http://dx.doi.org/10.1016/j.talanta.2023.124645.

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46

Rohner, Peter, Esther I. M. Jahn, Beatrice Ninet, et al. "Rapid Diagnosis of Pulmonary Tuberculosis with the LCx Mycobacterium tuberculosis Assay and Comparison with Conventional Diagnostic Techniques." Journal of Clinical Microbiology 36, no. 10 (1998): 3046–47. http://dx.doi.org/10.1128/jcm.36.10.3046-3047.1998.

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The LCx MTB amplification assay is a nucleic acid amplification test intended for the direct detection ofMycobacterium tuberculosis complex in respiratory specimens. We evaluated its performance on 2,001 consecutive respiratory specimens; 78 were culture positive for M. tuberculosis. Sensitivity, specificity, and positive and negative predictive values of this assay for all specimens compared to culture results were 88.5, 97.7, 60.5, and 99.5%, respectively. When referred to resolved clinical diagnosis of active tuberculosis, these values improved to 90.2, 98.4, 72.8, and 99.5%, respectively.

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Courtney, Samantha J., Zachary R. Stromberg, and Jessica Z. Kubicek-Sutherland. "Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza." Biosensors 11, no. 2 (2021): 47. http://dx.doi.org/10.3390/bios11020047.

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Influenza virus poses a threat to global health by causing seasonal outbreaks as well as three pandemics in the 20th century. In humans, disease is primarily caused by influenza A and B viruses, while influenza C virus causes mild disease mostly in children. Influenza D is an emerging virus found in cattle and pigs. To mitigate the morbidity and mortality associated with influenza, rapid and accurate diagnostic tests need to be deployed. However, the high genetic diversity displayed by influenza viruses presents a challenge to the development of a robust diagnostic test. Nucleic acid-based tes

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Zhao, Jinbin, Yulan Guo, Xueer Ma, et al. "The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens." Foods 12, no. 22 (2023): 4067. http://dx.doi.org/10.3390/foods12224067.

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Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy. This has led to the development of more sensitive and accurate methods for detecting pathogenic bacteria. Many signal amplification techniques have been used to improve the sensitivity of foodborne pathogen detection. Among them, hybridization chain reaction (HCR), an isothermal nucleic acid hybridization signal amplification technique, has received inc

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PETERKA, Matjaž, Aleš GASPARIČ, and Gorazd AVGUŠTIN. "A rapid nucleic acid solution concentration technique using N2 flushing." Acta agriculturae Slovenica 70, no. 1 (1997): 63–68. http://dx.doi.org/10.14720/aas.1997.70.1.16144.

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A rapid, inexpensive and efficient nucleic acid solution concentration method is described. The volume of the solution was reduced and the concentration of the nucleic acids in the solution increased by flushing the solution with nitrogen gas at the flow of 2-3 l/minute. The method was compared with the commonly used precipitation technique and a similar efficiency was established for both methods, the flushing technique being however faster. The quality of the nucleic acids did not change during the concentration procedure which was proved by successful endonuclease restriction of the materia

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Jia, Yijing, Xiaotong Shen, Feifei Sun, Na Na, and Jin Ouyang. "Metal–DNA coordination based bioinspired hybrid nanospheres for in situ amplification and sensing of microRNA." Journal of Materials Chemistry B 8, no. 48 (2020): 11074–81. http://dx.doi.org/10.1039/d0tb02315a.

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Based on coordination-driven self-assembly techniques, a facile approach for the intracellular delivery of nucleic acid into living cells and in situ intracellular targeted miRNA bioimaging using synthesized nano-wire balls (NWs) is proposed.

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