Relevant bibliographies by topics / ORF2 protein

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'ORF2 protein'

Author: Grafiati
Published: 15 June 2024
Last updated: 31 July 2025

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Journal articles on the topic "ORF2 protein"

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Tsai, Chi-Wei, Margaret G. Redinbaugh, Kristen J. Willie, Sharon Reed, Michael Goodin, and Saskia A. Hogenhout. "Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins." Journal of Virology 79, no. 9 (2005): 5304–14. http://dx.doi.org/10.1128/jvi.79.9.5304-5314.2005.

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ABSTRACT The genome of the nucleorhabdovirus maize fine streak virus (MFSV) consists of 13,782 nucleotides of nonsegmented, negative-sense, single-stranded RNA. The antigenomic strand consisted of seven open reading frames (ORFs), and transcripts of all ORFs were detected in infected plants. ORF1, ORF6, and ORF7 had significant similarities to the nucleocapsid protein (N), glycoprotein (G), and polymerase (L) genes of other rhabdoviruses, respectively, whereas the ORF2, ORF3, ORF4, and ORF5 proteins had no significant similarities. The N (ORF1), ORF4, and ORF5 proteins localized to nuclei, con
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Kondo, Hideki, Takanori Maeda, Yukio Shirako, and Tetsuo Tamada. "Orchid fleck virus is a rhabdovirus with an unusual bipartite genome." Journal of General Virology 87, no. 8 (2006): 2413–21. http://dx.doi.org/10.1099/vir.0.81811-0.

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Orchid fleck virus (OFV) has an unusual bipartite negative-sense RNA genome with clear sequence similarities to those of nucleorhabdoviruses. The OFV genome consists of two single-stranded RNA molecules, RNA1 and RNA2 that are 6413 and 6001 nt long, respectively, with open reading frame (ORF) information in the complementary sense. RNA1 encodes 49 (ORF1), 26 (ORF2), 38 (ORF3), 20 (ORF4) and 61 kDa (ORF5) proteins, and RNA2 encodes a single protein of 212 kDa (ORF6). ORF1, ORF5 and ORF6 proteins had significant similarities (21–38 % identity) to the nucleocapsid protein (N), glycoprotein (G) an
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Melzer, M. J., A. V. Karasev, D. M. Sether, and J. S. Hu. "Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2." Journal of General Virology 82, no. 1 (2001): 1–7. http://dx.doi.org/10.1099/0022-1317-82-1-1.

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The genome of pineapple mealybug wilt-associated closterovirus-2 (PMWaV-2) was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined. The 3′-terminal 14861 nt of the single-stranded RNA genome contains ten open reading frames (ORFs) which, from 5′ to 3′, potentially encode a >204 kDa polyprotein containing papain-like protease, methyltransferase and helicase domains (ORF1a), a 65 kDa RNA-dependent RNA polymerase (ORF1b), a 5 kDa hydrophobic protein (ORF2), a 59 kDa heat shock protein 70 homologue (ORF3), a 46 kDa protein (ORF4), a 34 kDa coat protein (
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Li, Jiapeng, Xiaoyin Wu, Hui Liu, et al. "Identification and Molecular Characterization of a Novel Carlavirus Infecting Chrysanthemum morifolium in China." Viruses 15, no. 4 (2023): 1029. http://dx.doi.org/10.3390/v15041029.

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Chrysanthemum (Chrysanthemum morifolium) is an important ornamental and medicinal plant suffering from many viruses and viroids worldwide. In this study, a new carlavirus, tentatively named Chinese isolate of Carya illinoinensis carlavirus 1 (CiCV1-CN), was identified from chrysanthemum plants in Zhejiang Province, China. The genome sequence of CiCV1-CN was 8795 nucleotides (nt) in length, with a 68-nt 5′-untranslated region (UTR) and a 76-nt 3′-UTR, which contained six predicted open reading frames (ORFs) that encode six corresponding proteins of various sizes. Phylogenetic analyses based on
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Shafat, Zoya. "Sequence to structural analysis of ORF5 protein in Norway rat Hepatitis E Virus." Bioinformation 18, no. 1 (2022): 19–25. http://dx.doi.org/10.6026/97320630018019.

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Hepatitis E virus (HEV) is a major causative agent of acute hepatitis in developing countries. The Norway rat HEV genome consists of six open reading frames (ORFs), i.e., ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6. The additional reading frame encoded protein ORF5 is attributed to life cycle of rat HEV. The ORFF5 protein’s function remains undetermined. Therefore, it is of interest to analyze the ORF5 protein for its physiochemical properties, primary structure, secondary structure, tertiary structure and functional characteristics using bioinformatics tools. Analysis of the ORF5 protein revealed i
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Shafat, Zoya. "Intrinsically disordered regions in the rodent hepevirus proteome." Bioinformation 18, no. 2 (2022): 111–18. http://dx.doi.org/10.6026/97320630018111.

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Hepatitis E virus (HEV) is the causative agent of Hepatitis E infections across the world. Intrinsically disordered protein regions (IDPRs) or intrinsically disordered proteins (IDPs) are regions or proteins that are characterized by lack of definite structure. These IDPRs or IDPs play significant roles in a wide range of biological processes, such as cell cycle regulation, control of signaling pathways, etc. IDPR/IDP in proteins is associated with the virus’s pathogenicity and infectivity. The prevalence of IDPR/IDP in rat HEV proteome remains undetermined. Hence, we examined the unstructured
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Garcia, Miguel, Madalena Pimentel та José Moniz-Pereira. "Expression of Mycobacteriophage Ms6 Lysis Genes Is Driven by Two σ70-Like Promoters and Is Dependent on a Transcription Termination Signal Present in the Leader RNA". Journal of Bacteriology 184, № 11 (2002): 3034–43. http://dx.doi.org/10.1128/jb.184.11.3034-3043.2002.

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ABSTRACT A mycobacteriophage Ms6 strong promoter region (Plys ) was isolated by using transcriptional fusions with the lacZ reporter gene. Two tandem σ70-like promoter sequences (P1 and P2) were found in this region. DNA sequencing of the promoter downstream region revealed a 214-bp leader sequence followed by five adjacent coding regions of 231 bp (ORF1), 1,152 bp (ORF2), 996 bp (ORF3), 231 bp (ORF4), and 372 (ORF5). ORF1 has the potential to encode a 77-amino-acid protein which revealed similarity to mycobacteriophage TM4 gp90, a predicted protein with unknown function. ORF2 encodes a 384-am
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Kiatpapan, Pornpimon, Yoshiteru Hashimoto, Hisako Nakamura, et al. "Characterization of pRGO1, a Plasmid from Propionibacterium acidipropionici, and Its Use for Development of a Host-Vector System in Propionibacteria." Applied and Environmental Microbiology 66, no. 11 (2000): 4688–95. http://dx.doi.org/10.1128/aem.66.11.4688-4695.2000.

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ABSTRACT The complete nucleotide sequence of pRGO1, a cryptic plasmid fromPropionibacterium acidipropionici E214, was determined. pRGO1 is 6,868 bp long, and its G+C content is 65.0%. Frame analysis of the sequence revealed six open reading frames, which were designated Orf1 to Orf6. The deduced amino acid sequences of Orf1 and Orf2 showed extensive similarities to an initiator of plasmid replication, the Rep protein, of various plasmids of gram-positive bacteria. The amino acid sequence of the putative translation product of orf3 exhibited a high degree of similarity to the amino acid sequenc
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Shimizu, Takeo, Hiroshi Kinoshita, and Takuya Nihira. "Identification and In Vivo Functional Analysis by Gene Disruption of ctnA, an Activator Gene Involved in Citrinin Biosynthesis in Monascus purpureus." Applied and Environmental Microbiology 73, no. 16 (2007): 5097–103. http://dx.doi.org/10.1128/aem.01979-06.

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ABSTRACT Citrinin, a secondary fungal metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. From the red-pigment producer Monascus purpureus, a 21-kbp region flanking pksCT, which encodes citrinin polyketide synthase, was cloned. Four open reading frames (ORFs) (orf1, orf2, orf3, and orf4) in the 5′-flanking region and one ORF (orf5) in the 3′-flanking region were identified in the vicinity of pksCT. orf1 to orf5 encode a homolog of a dehydrogenase (similarity, 46%), a regulator (similarity, 38%), an oxygenase (similarity, 41%), an oxidoreductase (similar
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Surjit, Milan, Shahid Jameel, and Sunil K. Lal. "The ORF2 Protein of Hepatitis E Virus Binds the 5′ Region of Viral RNA." Journal of Virology 78, no. 1 (2004): 320–28. http://dx.doi.org/10.1128/jvi.78.1.320-328.2004.

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ABSTRACT Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. Using the yeast three-hybrid system and traditional biochemical techniques, we have studied the RNA binding activities of ORF2 and ORF3, two proteins encoded in the 3′ structural part of the genome. Since the genomic RNA from HEV has been postulated to contain sec
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Dissertations / Theses on the topic "ORF2 protein"

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Guo, Hailong. "Antigenic epitope composition and protectivity of avian hepatitis E virus (avian HEV) ORF2 protein and vertical transmission of avian HEV." [Ames, Iowa : Iowa State University], 2006.

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Ankavay, Maliki. "Étude des modifications post-traductionnelles de la protéine de capside ORF2 du virus de l'hépatite E (HEV)." Thesis, Lille 2, 2019. http://www.theses.fr/2019LIL2S013.

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L’infection par le virus de l’hépatite E (HEV) est un problème majeur de santé publiquequi touche plus de 20 millions de personnes et tue environ 70 000 chaque année à travers lemonde. Le HEV est la cause majeure d’hépatite virale aiguë dans le monde. En France, laséroprévalence du HEV est de 22,4% dans la population générale. Ce virus se transmet parvoie féco-orale ou par consommation de viande contaminée mal cuite. Très récemment, nousavons décrit un système de culture cellulaire permettant d’amplifier efficacement le HEV. Cesystème représente un outil unique pour caractériser les différente
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Ferrié, Martin. "Étude de la maturation protéolytique et du trafic intracellulaire de la protéine de capside ORF2 du virus de l'hépatite E (HEV)." Electronic Thesis or Diss., Université de Lille (2022-....), 2023. http://www.theses.fr/2023ULILS067.

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L'infection par le virus de l'Hépatite E (HEV) est un problème majeur de santé publique qui toucherait 100 millions de personnes et tuerait 100 000 personnes chaque année dans le monde. Le HEV est la première cause d'hépatite aigüe dans le monde. En France, la séroprévalence s'élève à 22,4%. Ce virus se transmet par voie féco-orale ou par la consommation de viande contaminée mal cuite. Au cours de ma thèse, je me suis intéressé plus particulièrement à la protéine de capside ORF2, qui est l'unité structurale des particules virales et un acteur central du cycle infectieux du HEV. La protéine ORF
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Horridge, Jackie J. "RNA-protein interactions of the adenovirus proteins E1B 55K and E4 Orf6." Thesis, University of Warwick, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322435.

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Baghbadorani, G. Ahmadian. "Analysis of the utilisation of second ORFs in pneumovirus mRNA." Thesis, University of Warwick, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323416.

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Chand, Puran. "Molecular and immunological characterisation of a major envelope protein of capripoxvirus." Thesis, University of Surrey, 1992. http://epubs.surrey.ac.uk/2774/.

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Analysis of the proteins of capripoxvirus (KS-1) revealed a 32kd protein that is one of the major structural proteins of the virus and is localised in the virus envelope. Monospecific serum prepared against the 32kd envelope protein neutralised the virus indicating that this protein contains neutralising epitopes. Lymphocyte proliferation studies, using the 32kd protein and peripheral blood mononuclear cells from capripoxvirus (KS-i) vaccinated sheep, showed that this protein strongly induced cellmediated immune responses. The 32kd protein is capripoxvirus specific and induced antibodies in ea
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Koyuncu, Emre. "Expression, Purification, And Functional Analysis Of Adenovirus Type 5 E4 Orf3 Protein." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/12605415/index.pdf.

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In this study, structural and functional aspects of adenovirus type 5 (Ad5) E4orf3 protein were analyzed by biophysical and biochemical methods. Ad5 is one of the mostly used gene therapy vectors to date. However, some of its proteins possess oncogenic potential and their presence comprises safety risks. E4orf3 is one of the oncoproteins of Ad5. It also takes important roles in viral infection, and is beneficial for therapy vectors. Therefore, understanding the functions of E4orf3 is very important for developing efficient and safe adenovirus vectors. Most of the present knowledge about the f
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Bowers, Laura Yvonne. "Orf protein modulates phage and bacterial pathways of genetic recombination." Thesis, Durham University, 2008. http://etheses.dur.ac.uk/2345/.

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The emergence of novel pathogenic organisms due to the acquisition of virulence determinants from bacteriophages has generated significant interest in the pathways responsible for genomic rearrangements. Phageλ encodes its own recombination system, the Red system, comprising Exo, β and γ proteins. In addition,λ encodes another recombinase, Orf, which participates in the initial stages of genetic exchange and supplies a frmction equivalent to that of the Escherichia coli RecFOR proteins. This thesis focuses on determining the function of Orf in phage and bacterial recombination pathways by anal
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Liedeman, Kerwin. "Transient transgene expression of human Coronavirus nl63 orf3 protein in a baculovirus system." University of the Western Cape, 2020. http://hdl.handle.net/11394/8042.

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>Magister Scientiae - MSc<br>Insect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract inf
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Yadav, Kush Kumar. "Genotype 1 hepatitis E virus (HEV) ORF4 protein enhances genotype 3 HEV replication." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1574781581580768.

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Books on the topic "ORF2 protein"

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Horridge, Jackie J. RNA-protein interactions of the adenovirus proteins E1B 55K and E4 Orf6. typescript, 1998.

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2

Manfred, Schlapp, PEN-Club Liechtenstein, and ORF Landesstudio Vorarlberg (Austria), eds. Das verrantene Gewissen: Unser Tun und Lassen wider besseres Wissen : ein öffentliches Symposion des PEN-Club Lichtenstein in Zusammenarbeit mit dem ORF Landesstudio Vorarlberg. PEN-Club Liechtenstein, 1989.

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Meng, X. J. Hepatitis E virus. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0048.

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Hepatitis E virus (HEV) is a small, non-enveloped, single-strand, positive-sense RNA virus of approximately 7.2 kb in size. HEV is classified in the family Hepeviridae consisting of four recognized major genotypes that infect humans and other animals. Genotypes 1 and 2 HEV are restricted to humans and often associated with large outbreaks and epidemics in developing countries with poor sanitation conditions, whereas genotypes 3 and 4 HEV infect humans, pigs and other animal species and are responsible for sporadic cases of hepatitis E in both developing and industrialized countries. The avian
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Book chapters on the topic "ORF2 protein"

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Cernooka, Elina, Janis Rumnieks, and Andris Kazaks. "Structural Characterization of a Single-Stranded DNA-Binding Protein: A Case Study of the ORF6 Protein from Bacteriophage Enc34." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1290-3_23.

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Yamakawa, Hisashi. "High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-232-2_3.

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Flint, S. J., and R. A. Gonzalez. "Regulation of mRNA Production by the Adenoviral E1B 55-kDa and E4 Orf6 Proteins." In Current Topics in Microbiology and Immunology. Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-662-05597-7_10.

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Nigam, Mohit, Ruchi Yadav, and Garima Awasthi. "In-Silico Construction of Hybrid ORF Protein to Enhance Algal Oil Content for Biofuel." In Advances in Biomedical Engineering and Technology. Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-6329-4_8.

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Czerny, Claus-Peter, Michaela Alex, Jana Pricelius, and Christiane Zeller-Lue. "Development of Quantitative PCR Tests for the Detection of the Orthopox Virus Adsorption Protein Gene (ORF D8L) on the LightCycler." In Rapid Cycle Real-Time PCR. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0_40.

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Ferro-Novick, S. "BET2." In Secretory Pathway. Oxford University PressOxford, 1994. http://dx.doi.org/10.1093/oso/9780198599425.003.0081.

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Abstract ORF2, an essential yeast gene of unknown function. The DNA sequences of BET2 and ORF2 are identical with the exception of one nucleotide alteration that results in a conserved amino acid change in the protein (asparagine in Orf2 and lysine in Bet2 at position 22)213. This change may be attributable to differences in strain backgrounds.
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Ferro-Novick, Susan. "Bet2p." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0015.

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Abstract The DNA sequence of BET2 predicts a hydrophilic protein of-36 500 daltons (Peterson-Bjorn et al. 1990; Rossi et al. 1991). Antibody raised to this protein has confirmed this prediction (Jiang et al. 1993). Bet2 shares 56.6% similarity and 34% identity with Dpr1 (Ram1), an essential component of a protein prenyltransferase that adds farnesyl (a 15 carbon lipid) to the COCH-terminus of Ras and the yeast mating pheromone afactor (Schafer et al. 1990; Goodman et al. 1990; He et al. 1991).8ET2wasalsofoundto be the same as ORF2, an essential yeast gene of unknown function (Peterson-Bjorn et
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Sriram, Anitha, Ravindra Vasave, Indrani Maji, et al. "Virology of SARS-CoV2." In An Update on SARS-CoV-2: Damage-response Framework, Potential Therapeutic Avenues and the Impact of Nanotechnology on COVID-19 Therapy Volume 1. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/9789815039863122010005.

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The 2019-nCoV RNA genome is highly protected by its unique structure equipped with mechanistic spike proteins on its surface. Its RNA genome contains almost all 14 Orfs encoding for at least 27 proteins. An Orf is a part of genetic material that can undergo translation and produce proteins. Four structural proteins (SPs) likely spike (S), envelope (E), membrane (M), and nucleocapsid (N) are present in 2019- nCoV and afford its structure. In this chapter, we have discussed in detail the virology of 2019-nCoV including structural proteins (SPs), accessory proteins, non-structural proteins (NSPs)
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Skolnick, Jeffrey, and Yang Zhang. "Protein Structure Prediction." In Systems Biology. Oxford University PressNew York, NY, 2006. http://dx.doi.org/10.1093/oso/9780195300819.003.0007.

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Abstract Over the past decade, the success of genome sequence efforts has brought about a paradigm shift in biology [1]. There is increasing emphasis on the large-scale, high-throughput examination of all genes and gene products of an organism, with the aim of assigning their functions [2]. Of course, biological function is multifaceted, ranging from molecular/biochemical to cellular or physiological to phenotypical [3]. In practice, knowledge of the DNA sequence of an organism and the identification of its open reading frames (ORFs) does not directly provide functional insight. Here, the focu
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Kondegowda, Nagesha Guthalu, Sheela Joshi Gokhale, George Harb, et al. "Parathyroid Hormone Related Protein (PTHrP) Enhances Human β-Cell Proliferation and Function with Simultaneous Induction of Cyclin-Dependent Kinase 2 (cdk2) and Cyclin E Expression." In The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego. Endocrine Society, 2010. http://dx.doi.org/10.1210/endo-meetings.2010.part3.or2.or32-1.

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Conference papers on the topic "ORF2 protein"

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Stoicescu, Ramona, Razvan-Alexandru Stoicescu, Codrin Gheorghe, Adina Honcea, and Iulian Bratu. "CONSIDERATIONS ON SARS-COV-2 DIAGNOSIS IN THE LABORATORY OF UNIVERSITY EMERGENCY CLINICAL HOSPITAL OF CONSTANTA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/07.

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Coronaviruses are members of the Coronaviridae family. They are enveloped, non-segmented, positive-sense, single-stranded RNA viruses. Their genome size is about 30 kilobases (kb) which consist, at the 5’ end, of non-structural open reading frames (ORFs: ORF1a, ORF 1b) which code for 16 non structural proteins, and at the 3’ end the genes which code for four structural proteins including membrane (M), envelope (E), spike (S), and nucleocapsid (N) proteins. Due to the rapid spread of COVID-19, a reliable detection method is needed for patient diagnosis especially in the early stages of the dise
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Yang, Fan, Hong-Liang Liu, Xiao-Wan Liu, et al. "Development of a TTSuV2-ORF1 Protein-based Indirect Blocking ELISA for Serological Testing." In 2015 International Conference on Medicine and Biopharmaceutical. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814719810_0023.

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Shan, Si, Nie Peng, Xiaojun Yan, and Caifeng Ba. "Study on the secondary structure and B-cell epitopes of Mycoplasma suis ORF9 protein by Bioinformatics." In 2015 International Power, Electronics and Materials Engineering Conference. Atlantis Press, 2015. http://dx.doi.org/10.2991/ipemec-15.2015.194.

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"Lettuce Big Vein associated Virus ORF3 encodes a 30K-like protein that facilitates cell-to-cell movement." In IS-MPMI Congress. IS-MPMI, 2023. http://dx.doi.org/10.1094/ismpmi-2023-50.

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Saksaganskaia, Alla S., Victoria S. Muntyan, Alexey N. Muntyan, Boris V. Simarov, and Marina L. Roumiantseva. "ABUNDANCE OF PHAGE-RELATED SEQUENCES ON NON-SYMBIOTIC PLASMIDS OF SINORHIZOBIUM MELILOTI FROM CENTERS OF LEGUME PLANTS DIVERSITY." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/6.1/s25.06.

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Genomes of alfalfa root nodule bacteria, Sinorhizobium meliloti, symbionts of alfalfa are enriched in non-symbiotic (cryptic) plasmids, which gene pool is remained weakly studied. S. meliloti strains are significantly varied in number and size of these plasmids. The goal of the study was to assess the occurrence of phage-related sequences (PRS) on cryptic plasmids. Whole genome sequences of 12 S. meliloti strains native to Caucasian and Kazakhstan centers of alfalfa diversity (NCG and PAG, correspondingly) were studied and 20 cryptic plasmids, which sizes varied from 17.2 to 453.8 kb, were ass
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Nogueira, Raniery Augusto dos Santos Beserra, Ana Beatriz Silva Barbosa, Francisco Das Chagas Diassis Jácome Valentim, Sâmya Pires Batista De Azevêdo, and Jamile Rodrigues Cosme De Holanda. "MECANISMOS DE MANUTENÇÃO DA LATÊNCIA DO VÍRUS VARICELA ZÓSTER: UMA ADAPTAÇÃO NECESSÁRIA." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1182.

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Introdução: O Vírus Varicela Zóster pertence à subfamília α-herpesviridae e é um DNA-vírus de fita dupla, possuindo 68 Fases de Leitura Aberta (ORF) únicas. Sendo responsável por varicela, em um primeiro contato, o vírus mantém-se latente por um longo período em gânglios das raízes dorsais e de nervos cranianos, disseminando-se por essas estruturas após décadas, quando reativado, configurando Herpes Zóster, cujas complicações prejudicam a qualidade de vida dos pacientes. Posteriormente à infecção primária, o vírus classifica-se como neurotrópico e seus meios de sobrevida em neurônios, ainda nã
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Silva, Victoria Regina Da, Gabriela Rodrigues De Aguiar, Jackson Nascimento De Souza, and Rhayanny Kethylly Pereira Santos. "CORRELAÇÃO DOS RETROVÍRUS ENDÓGENOS HUMANOS (HERVS) COM A SEVERIDADE DOS CASOS DE COVID-19 ABRE A POSSIBILIDADE DE SEREM USADOS COMO MARCADORES DE PROGNÓSTICO NA DOENÇA." In I Congresso Brasileiro de Imunologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1150.

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Introdução: A COVID-19 é uma doença pandêmica com graves consequências socioeconômicas causada pelo vírus SARS-COV-2. Ela é caracterizada por sintomas respiratórios leves a graves, como pneumonia e falência de órgãos. Comorbidades e idade avançada são fatores de risco para a doença, todavia, a intensidade da doença não é explicada somente por esses fatores, o que evidencia que mecanismos ainda desconhecidos contribuem no seu prognóstico. HERVs são fragmentos virais incorporados no genoma humano que agravam danos teciduais quando superexpressos em algumas condições patológicas, como neurodegene
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Reports on the topic "ORF2 protein"

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ธนียวัน, จิราภรณ์, กอบชัย ภัทรกุลวณิชย์ та ขนิษฐา วงษ์นิกร. การโคลนและลักษณะสมบัติของยีนกลุ่ม rhl ที่เกี่ยวข้องกับการสังเคราะห์โมโนแรมโนลิพิดทางชีวภาพของ Pseudomonas aeruginosa A41 : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2006. https://doi.org/10.58837/chula.res.2006.41.

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Pseudomonas sp. A41 ที่แยกจากน้ำทะเลอ่าวไทย มีความสามารถในการผลิตแรมโนลิพิดจากลักษณะทางสัณฐานวิทยาและสมบัติทางชีวเคมีร่วมกับข้อมูลลำดับนิวคลีโอไทด์ของ 16S rDNA สามารถจำแนกสายพันธุ์ A41 เป็น Pseudomonas aeruginosa แยกยีนที่เกี่ยวข้องกับการสังเคราะห์แรมโนลิพิดจาก Pseudomonas aeruginosa A41 โดยเทคนิคเซาท์เธอร์นไฮบริไดเซชันจีโนมิกดีเอ็นเอกับดีเอ็นเอติดตาม rhlR หรือ rhlA โคลนชิ้นดีเอ็นเอที่ให้ผลบวกเข้าในพลาสมิดเวกเตอร์และหาลำดับนิวคลีโอไทด์ของชิ้นดีเอ็นเอแทรกสอด จากลำดับนิวคลีโอไทด์ทั้งสิ้น 4,965 bp พบกรอบอ่านรหัสเปิด (ORF) ที่สมบูรณ์จำนวน 4 แห่ง และไม่สมบูรณ์ 1 แห่ง กรอบอ่านรหัสเปิดทั้งหมดมีทิศทาง
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Gafny, Ron, A. L. N. Rao, and Edna Tanne. Etiology of the Rugose Wood Disease of Grapevine and Molecular Study of the Associated Trichoviruses. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575269.bard.

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Rugose wood is a complex disease of grapevines, characterized by modification of the woody cylinder of affected vines. The control of rugose wood is based on the production of healthy propagation material. Detection of rugose wood in grapevines is difficult and expensive: budwood from tested plants is grafted onto sensitive Vitis indicators and the appearance of symptoms is monitored for 3 years. The etiology of rugose wood is complex and has not yet been elucidated. Several elongated clostero-like viruses are consistently found in affected vines; one of them, grapevine virus A (GVA), is close
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Coplin, David, Isaac Barash, and Shulamit Manulis. Role of Proteins Secreted by the Hrp-Pathways of Erwinia stewartii and E. herbicola pv. gypsophilae in Eliciting Water-Soaking Symptoms and Initiating Galls. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7580675.bard.

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Many bacterial pathogens of plants can inject pathogenicity proteins into host cells using a specialized type III secretion system encoded by hrpgenes. This system deliver effector proteins, into plant cells that function in both susceptible and resistant interactions. We have found that the virulence of Erwinia stewartii(Es; syn. Pantoea stewartii) and Erwinia herbicola pv. gypsophilae (Ehg, syn. Pantoea agglomerans), which cause Stewart's wilt of corn and galls on Gypsophila, respectively, depends on hrpgenes. The major objectives of this project were: To increase expression of hrpgenes in o
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Barefoot, Susan F., Bonita A. Glatz, Nathan Gollop, and Thomas A. Hughes. Bacteriocin Markers for Propionibacteria Gene Transfer Systems. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573993.bard.

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The antibotulinal baceriocins, propionicin PLG-1 and jenseniin G., were the first to be identified, purified and characterized for the dairy propionibaceria and are produced by Propionibacterium thoenii P127 and P. thoenii/jensenii P126, respectively. Objectives of this project were to (a) produce polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1; (b) identify, clone and characterize the propionicin PLG-1 (plg-1) and jenseniin G (jnG) genes; and (3) develop gene transfer systems for dairy propionibacteria using them as models. Polyclonal antibodies for detecti
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อิ่มจงใจรัก, จันทร์ประภา. การแยกและการศึกษาลักษณะสมบัติของเปปไทด์ต้านจุลชีพจากเซลล์เม็ดเลือดของปูทะเล Scylla paramamosain : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.52.

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เปปไทด์ต้านจุลชีพเป็นเปปไทด์สายสั้นที่มีความสำคัญต่อระบบภูมิคุ้มกันของร่างกายของสิ่งมีชีวิตหลายชนิดโดยมีคุณสมบัติที่สามารถต่อต้านการติดเชื้อที่เกิดจากจุลชีพได้ ในงานวิจัยนี้ได้ทำการค้นหาลำดับนิวคลีโอไทด์ที่สมบูรณ์(full length cDNA) ของยีนเปปไทด์ต้านจุลชีพครัสติน(CrusSp)ศึกษาลักษณะสมบัติของยีนรวมทั้งแยกและวิเคราะห์การจัดเรียงตัวของยีน(genomicorganization)จากการค้นหาลำดับนิวคลีโอไทด์ที่สมบูรณ์ของยีนเปปไทด์ต้านจุลชีพครัสตินจากเซลล์เม็ดเลือดแดงของปูทะเล(Scylla paramamosain) โดยเทคนิค expressed sequence tag(EST) และ rapid amplification cDNA end (RACE) พบว่าประกอบด้วย ORF ขนาด 336 bp ที่สามารถถอดรหั
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สิทธิประณีต, ศิริพร. การสร้าง E.Coli ที่สามารถผลิตโปรตีนหลักรอยัลเจลลี ของผึ้งด้วยการโคลนดีเอ็นเอ : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2004. https://doi.org/10.58837/chula.res.2004.45.

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ได้โคลน cDNA ของ Major Jelly Protein ของผึ้งโพรง Apis cerana (AcMRJP) โดยใช้ผลิตภัณฑ์จาก TR-PCR หลังศึกษาสมบัติเฉพาะพบว่าได้โคลนของ MRJP1 และ MRJP2 โดย Open Reading Frames (ORF) ของ AcMPJP1 และ MRJP2 มีขนาด 1302 และ 1392 นิวคลีโอไทด์ ซึ่งระบุการถอดรหัสเป็นกรดอะมิโน 433 และ 463 residues ตามลำดับ ความคล้ายคลึงของ MRCJP1 และ MRCJP2 ระหว่างผึ้งโพรงและผึ้งพันธุ์ (Apis mellifera) เป็น 93 และ 92 เปอร์เซ็นต์ สำหรับลำดับนิวคลีโอไทด์ และเป็น 90 และ 85 เปอร์เซ็นต์ สำหรับกรดอะมิโนตามลำดับ เมื่อ subclone cDNA ของ AcMRJP1 โดยใช้ expression vector, pET17b และทรานสฟอร์มเข้าสู่ E. coli Rosetta (DE3) pLys S พบก
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Ostersetzer-Biran, Oren, and Alice Barkan. Nuclear Encoded RNA Splicing Factors in Plant Mitochondria. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7592111.bard.

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Mitochondria are the site of respiration and numerous other metabolic processes required for plant growth and development. Increased demands for metabolic energy are observed during different stages in the plants life cycle, but are particularly ample during germination and reproductive organ development. These activities are dependent upon the tight regulation of the expression and accumulation of various organellar proteins. Plant mitochondria contain their own genomes (mtDNA), which encode for a small number of genes required in organellar genome expression and respiration. Yet, the vast ma
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Dawson, William O., Moshe Bar-Joseph, Charles L. Niblett, Ron Gafny, Richard F. Lee, and Munir Mawassi. Citrus Tristeza Virus: Molecular Approaches to Cross Protection. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570551.bard.

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Citrus tristeza virus (CTV) has the largest genomes among RNA viruses of plants. The 19,296-nt CTV genome codes for eleven open reading frames (ORFs) and can produce at least 19 protein products ranging in size from 6 to 401 kDa. The complex biology of CTV results in an unusual composition of CTV-specific RNAs in infected plants which includes multiple defective RNAs and mixed infections. The complex structure of CTV populations poses special problems for diagnosis, strain differentiation, and studies of pathogenesis. A manipulatable genetic system with the full-length cDNA copy of the CTV gen
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Antignus, Yehezkiel, Ernest Hiebert, Shlomo Cohen, and Susan Webb. Approaches for Studying the Interaction of Geminiviruses with Their Whitefly Vector Bemisia tabaci. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604928.bard.

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The DNA of tomato yellow leaf curl virus (TYLCB) was detected in its whitefly vector, Bemisia tabaci, by dot spot hybridization as early as 1 h after acquisition access. The retention of the virus nucleic acid in the vector was at least 23 days after a 48 h acquisition access. However, the retention of TYLCV coat protein did not exceed 10 days. No replicative forms of TYLCV could be detected in B. tabaci, indicating a non-propagative relationship with the vector. Whiteflies were not able to accumulate naked virion ssDNA, virus cloned dsDNA, or virions with impaired coat protein. Deletion, fram
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Ostersetzer-Biran, Oren, and Jeffrey Mower. Novel strategies to induce male sterility and restore fertility in Brassicaceae crops. United States Department of Agriculture, 2016. http://dx.doi.org/10.32747/2016.7604267.bard.

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Abstract Mitochondria are the site of respiration and numerous other metabolic processes required for plant growth and development. Increased demands for metabolic energy are observed during different stages in the plants life cycle, but are particularly ample during germination and reproductive organ development. These activities are dependent upon the tight regulation of the expression and accumulation of various organellar proteins. Plant mitochondria contain their own genomes (mtDNA), which encode for rRNAs, tRNAs and some mitochondrial proteins. Although all mitochondria have probably evo
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