Academic literature on the topic 'P fluorescens rdna sequencing'

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Journal articles on the topic "P fluorescens rdna sequencing"

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Fu, Yan-Mei, Hong-Bin Liu, and Xiao-Qin Wu. "Diversity and Function of Endo-Bacteria in Bursaphelenchus xylophilus from Pinus massoniana Lamb. in Different Regions." Forests 11, no. 5 (2020): 487. http://dx.doi.org/10.3390/f11050487.

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The pine wood nematode (PWN) Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease (PWD), a devastating forest disease. PWN-associated bacteria may play a role in PWD. However, little is known about the endo-bacteria in PWN. We analyzed the diversity of endo-bacteria in nine isolates of PWNs from Pinus massoniana Lamb. in nine epidemic areas from three Chinese provinces by high-throughput sequencing of 16S rDNA and isolated and identified culturable endo-bacteria through construction of a 16S rDNA phylogenetic tree and Biolog microbial identification. We also examined the ef
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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified
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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose
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Hassanshahian, Mehdi. "Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils." Iranian Journal of Toxicology 10, no. 3 (2016): 13–20. http://dx.doi.org/10.32598/ijt.10.4.357.1.

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Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA. Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environm
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Milijasevic-Marcic, Svetlana, Biljana Todorovic, Milos Stepanovic, et al. "Monitoring of bacterial diseases of Agaricus bisporus in Serbia." Pesticidi i fitomedicina 31, no. 1-2 (2016): 29–35. http://dx.doi.org/10.2298/pif1602029m.

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Monitoring of button mushroom bacterial diseases was conducted to estimate the presence and identity of mycopathogenic bacteria and to determine the predominant bacterial pathogen in Serbia. Samples were collected from mushroom farms during 2006- 2010 and also from fresh markets during 2014-2015. The collected samples showed either symptoms of brown blotch or different degrees of brown discoloration on caps and stalks of Agaricus bisporus resembling bacterial infection. The presence of bacterial droplets on gills was not recorded. The isolated bacteria were Gram-negative and fluorescent on Kin
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Marques, Eder, Rafaela CF Borges, and Carlos H. Uesugi. "Identification and pathogenicity of Pseudomonas cichorii associated with a bacterial blight of gerbera in the Federal District." Horticultura Brasileira 34, no. 2 (2016): 244–48. http://dx.doi.org/10.1590/s0102-053620160000200015.

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ABSTRACT: In 2013, leaf samples of gerbera plants showing symptoms of bacterial blight were collected in cut-flower high tunnels, in the settlement of núcleo rural alexandre gusmão, located in brazlândia, distrito federal, brazil. seven isolates obtained were subjected to phenotypic and molecular characterization, including pathogenicity tests, lopat, and partial sequencing of the 16s rdna gene. all isolates were gram-negative, aerobic, oxidase-positive, produced fluorescent pigment, induced hypersensitivity in tobacco leaves, used sorbitol and glutamate and were pathogenic to 24 different pla
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Li, Binbin, Shuji Liu, Xiaoting Chen, et al. "Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage." Foods 12, no. 21 (2023): 3994. http://dx.doi.org/10.3390/foods12213994.

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The quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker (Larimichthys crocea) during 4 °C refrigeration were studied using 16S rDNA high-throughput sequencing technology, and the total viable count (TVC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) were determined. The results revealed a consistent increase in TVC, TVB-N, and TBARS levels over time. On the 9th day, TVC reached 7.43 lg/(CFU/g), while on the 15th day, TVB-N exceeded the upper limit for acceptable quality, reaching 42.56 mg/100 g.
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Sharma, Garima, Shweta Dang, Sanjay Gupta, and Reema Gabrani. "IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BACTERIA HAVING ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 10 (2016): 111. http://dx.doi.org/10.22159/ijpps.2016v8i10.12338.

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<p><strong>Objective:</strong> The aim of the current study was to isolate and identify the bacteriocinogenic strain exhibiting broad range antimicrobial activity and antibiofilm activity from soil of animal farms.</p><p><strong>Methods:</strong> In the current study, bacterial strains were isolated from soil of twelve different regions of animal farm all over India and screened for antimicrobial activity against<em> Staphylococcus epidermidis</em>, <em>Micrococcus luteus, Pseudomonas fluorescence</em> and <em>Escherichia
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Koike, Steven T., Jeri D. Barak, Diana M. Henderson, and Robert L. Gilbertson. "Bacterial Blight of Leek: A New Disease in California Caused by Pseudomonas syringae." Plant Disease 83, no. 2 (1999): 165–70. http://dx.doi.org/10.1094/pdis.1999.83.2.165.

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During 1996 and 1997, a new and damaging disease of leek (Allium porrum) was observed on greenhouse-produced transplants and field-grown plants in California. Symptoms were water-soaked lesions at leaf tips, which eventually expanded down the length of the leaf and resulted in brown, elongated, stripe-like lesions with yellow margins. Diseased leaves eventually wilted. A blue fluorescent pseudomonad was consistently recovered from lesions, and biochemical and physiological tests indicated that it was Pseudomonas syringae. Pathogenicity tests established that representative strains of this P. s
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Akhtar, Khalid Pervaiz, Muhammad Yussouf Saleem, Sumaira Yousaf, Najeeb Ullah, Ghulam Rasool, and Nighat Sarwar. "Occurrence, identification and transmission of the phytoplasma associated with tomato big bud disease and identification of its vector and weed host in Pakistan." Archives of Phytopathology and Plant Protection 51, no. 7 (2018): 387–98. http://dx.doi.org/10.1080/03235408.2018.1485827.

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Tomato (Solanum lycopersicum L.) plants showing stunting, big bud, leaves yellowing or reddening and witches’-broom symptoms were observed since 2009 in Pakistan. A weed Parthenium hysterophorus grown in and around tomato fields also exhibited witches’-broom like symptoms. Fluorescence light microscopy of hand-cut stem stalk sections treated with Dienes’ stain showed blue areas in the phloem region of both tomato and P. hysterophorus symptomatic plants that indicated the association of phytoplasma with the complex. Amplification of 1.2 kb 16S rDNA fragment in nested PCR confirmed that the symp
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Book chapters on the topic "P fluorescens rdna sequencing"

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Poole Sophie, Singhrao Sim K., Chukkapalli Sasanka, et al. "Active Invasion of Porphyromonas gingivalis and Infection-Induced Complement Activation in ApoE." In Advances in Alzheimer’s Disease. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-706-1-119.

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Periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of Alzheimer's disease. ApoE−/−mice were orally infected (n=12) with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum as mono-and polymicrobial infections. ApoE−/−mice were sacrificed following 12 and 24 weeks of chronic infection. Bacterial genomic DNA was isolated from all brain tissues except for the F. nu
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