Relevant bibliographies by topics / PCR-RFLP markers

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Academic literature on the topic 'PCR-RFLP markers'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "PCR-RFLP markers"

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Dolanská, L., and V. Čurn. "Identification of white clover (Trifolium repens L.) cultivars using molecular markers." Plant, Soil and Environment 50, No. 3 (2011): 95–100. http://dx.doi.org/10.17221/4013-pse.

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The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability o
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Savichtcheva, O., and S. Okabe. "Qualitative and quantitative estimation of host-specific fecal pollution using Bacteroides–Prevotella 16S rRNA genetic markers by T-RFLP and real-time PCR analyses." Water Science and Technology 59, no. 9 (2009): 1831–40. http://dx.doi.org/10.2166/wst.2009.213.

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Rapid and reliable determination of the non-point sources of fecal pollution is a critical issue for the environmental microbiologists all over the world. In this work we evaluated the use of anaerobic bacterial group Bacteroides–Prevotella as an alternative fecal pollution indicator. Terminal restriction fragment length polymorphism (T-RFLP) and real-time polymerase chain reaction (RT-PCR) analyses were used to monitor and quantify human-, cow- and pig-specific fecal contamination in natural river waters. We also included DNA sequence analysis of the identified fecal markers revealed by T-RFL
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Mayes, S., P. L. Jack, R. H. V. Corley, and D. F. Marshall. "Construction of a RFLP genetic linkage map for oil palm (Elaeis guineensis Jacq.)." Genome 40, no. 1 (1997): 116–22. http://dx.doi.org/10.1139/g97-016.

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We have developed a restriction fragment length polymorphism (RFLP) genetic map in oil palm (Elaeis guineensis Jacq.) for use in breeding programmes. A segregating population of 98 individuals was probed with 84 informative low copy clones (mainly PstI genomics). This yielded 103 scorable loci, of which 97 could be linked into 24 groups of two or more markers (n = 16 for oil palm), encompassing a total of 860 cM. The high level of linkage between markers (95%) suggests good genome coverage and very little segregation distortion of markers was observed. The mapping population, which was generat
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GHIMIRE, S. C., and J. R. EGERTON. "PCR–RFLP of outer membrane proteins gene of Dichelobacter nodosus: a new tool in the epidemiology of footrot." Epidemiology and Infection 122, no. 3 (1999): 521–28. http://dx.doi.org/10.1017/s0950268899002290.

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Currently only phenotypic epidemiological markers, serogrouping and virulence testing of Dichelobacter nodosus, are available for investigating footrot outbreaks in small ruminants. These methods have limitations in tracing the source of infection. In this study, a genotypic marker, PCR–RFLP of outer membrane protein gene, was used to characterize D. nodosus. The technique was evaluated in a controlled experiment involving two strains of bacteria. PCR–RFLP was found to be highly specific in differentiating isolates obtained from recipient animals infected with different strains. Subsequently,
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Kauserud, Håvard, and Trond Schumacher. "Population structure of the endangered wood decay fungus Phellinus nigrolimitatus (Basidiomycota)." Canadian Journal of Botany 80, no. 6 (2002): 597–606. http://dx.doi.org/10.1139/b02-040.

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The population structure of five Fennoscandian geographic populations of the endangered wood-decay fungus Phellinus nigrolimitatus (Romell) Bourdot et Galzin was examined by analyses of nuclear ribosomal DNA (nrDNA) spacer sequences (ITS and IGS1) and a partial sequence of the elongation factor 1α gene (efa). A high level of sequence variation was observed in ITS and IGS1, suggesting restrictions in nrDNA homogenization in this taxon. Six polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) markers, five located in nrDNA and one in efa, suggest that the geographic po
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Mudaningrat, Ajeng, Flora Umaya, Farah Ayu Afdhila Syahriza, Yustinus Ulung Anggraito, and Ning Setiati. "LITERATURE REVIEW: APLIKASI PENANDA MOLEKULER UNTUK ANALISIS KEANEKARAGAMAN GENETIK HEWAN." BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan 10, no. 1 (2023): 11–25. http://dx.doi.org/10.30598/biopendixvol10issue1page11-25.

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Background: Molecular markers (DNA markers) are DNA sequences that are easily detected to characterize or detect certain genetic diversity, one of which is in animals. There are various marker systems used with their respective characteristics. Some of the molecular markers include RFLP, RAPD, ISSR, SSR, and SNP. The purpose of this study was to analyze genetic diversity in animals using molecular marker applications.Methods: The method used is literature review using reputable national and international journals.Results: Based on the results of a literature review, the genetic diversity of va
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VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe
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Nuroniah, H. S., O. Gailing, and R. Finkeldey. "Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)." Silvae Genetica 59, no. 1-6 (2010): 249–57. http://dx.doi.org/10.1515/sg-2010-0035.

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AbstractThe development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been described earlier showing strong differentiation between S. leprosula and S. parvifol
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Shibuya, H., B. K. Collins, S. J. Stoy, D. Nonneman, and G. S. Johnson. "PCR/RFLP markers in the canine gamma-crystallin gene." Animal Genetics 26, no. 6 (2009): 445–46. http://dx.doi.org/10.1111/j.1365-2052.1995.tb02700.x.

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Khatoon, Arifa, Sumeet Verma, Gayatri Wadiye, and Anuprita Zore. "Molecular markers and their potentials." International Journal of Bioassays 5, no. 01 (2016): 4706. http://dx.doi.org/10.21746/ijbio.2016.01.003.

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The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant molecular biotechnology and their genetic studies. There are three different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiating in two types 1. Non PCR based (RFLP) and 2. PCR based markers (RAPD, AFLP, SSR, SNP etc.). Amongst others, the microsatellite DNA marker is one of the most widely used marker due to its easy use by simple PCR, followed by a denaturing gel electrophoresis. SNP (Single Nucleotide Po
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Dissertations / Theses on the topic "PCR-RFLP markers"

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Vitaliano, Sérgio Netto. "Isolamento e caracterização biológica e genotípica de Toxoplasma gondii em animais selvagens do Brasil." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04102012-182312/.

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Toxoplasma gondii é um protozoário formador de cistos capaz de infectar diversas espécies de aves e mamíferos domésticos e selvagens, incluindo o homem. Apesar de evidências sorológicas da infecção por T. gondii em animais selvagens, pouco se sabe sobre o papel da vida selvagem na cadeia epidemiológica e tampouco a susceptibilidade das variadas espécies a este parasito. O presente trabalho consistiu no isolamento e caracterização genotípica de T. gondii de tecidos de animais selvagens, de vida livre e de cativeiro, provenientes de diversas localidades do Brasil e na detecção sorológica de anti
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Yang, Wei-Yin, and 楊衛崟. "Studies on Identification of Mitochondrial DNA PCR-RFLP in Common Grouper Species and Screening Microsatellite DNA Molecular Markers Associated with Growth Trait in Giant Grouper (Epinephelus lanceolatus)." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/fu934f.

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碩士<br>國立臺灣海洋大學<br>水產養殖學系<br>103<br>Groupers are important commercial aquaculture species in Asia, and giant grouper (Epinephelus lanceolatus) is the main species cultured in Taiwan. Giant grouper has advantages of large body size, fast growth, and thick meat with high economic value. More than 90% of Taiwanese groupers are exported to markets in China, Hong Kong, and locally with the upgrading of aquaculture techniques and increased production. This high production will generate oversupply, leading to price declines of some fish. Therefore, breeding new varieties of high-priced groupers is imp
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STEBLOVÁ, Halina. "Vliv vybraných kandidátních lokusů na ukazatele jakosti masa u skotu." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-180790.

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This study was aimed on analysis of polymorphism at position 2141 in exon 5 of the growth hormone gene (GH) and polymorphism at position 257 in exon 10 of the growth hormone receptor gene (GHR) and to determine the influence of these polymorphisms on meat tenderness. To analysis has been used 333 meat samples of Czech Pied bulls. Genotypization of GH and GHR loci was performed by PCR-RFLP. For detection of single nucleotide polymorphism in both genes was used restriction endonuclease AluI. To determine meat tenderness was used the method of measuring shear force by Warner and Bratzler. The she
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Books on the topic "PCR-RFLP markers"

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Studies on Genetic Relationships among Six Varieties of Jackfruit in Kerala Employing the Matk Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Studies on Genetic Relationships among Locally Cultivated Musaceae Varieties in Kerala Employing Rbcl and Matk Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Studies on Genetic Relationships among Locally Cultivated Citrus Varieties in Kerala Employing Matk and Rbcl Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Book chapters on the topic "PCR-RFLP markers"

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Bouayed Abdelmoula, Nouha, Samir Aloulou, Sonda Kammoun, et al. "PCR assay using 22q11.2 highly polymorphic markers for exclusion of 22q microdeletion: Technical optimization and application in North Africa." In Advances in Genetic Polymorphisms [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.1002331.

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22q11.2 deletion syndrome is a genomic disorder with a broader clinical and genetic spectrum. To exclude the presence of 22q11.2 microdeletion, we optimize a PCR-RFLP analysis of three SNP located in the typically proximal 22q11.21 deleted region of 1.5 Mb. PCR reactions, optimized with a Touch-Down program, were performed using three pairs of primers. The amplicons were cleaved by three restrictive enzymes: HaeIII, CviAII, and BsrI applied respectively, for rs4819523, rs4680, and rs5748411. The efficiency of this PCR RFLP assay was confirmed in the light of its application in a small cohort o
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Komori, T., T. Yamamoto, and N. Nitta. "Converting rice RFLP markers to PCR-based markers by the dCAPS method." In Advances in Rice Genetics. World Scientific Publishing Company, 2008. http://dx.doi.org/10.1142/9789812814319_0084.

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Rowe, Graham, Michael Sweet, and Trevor J. C. Beebee. "Molecular tools for molecular ecologists." In An Introduction to Molecular Ecology. Oxford University Press, 2017. http://dx.doi.org/10.1093/hesc/9780198716990.003.0003.

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This chapter explores the molecular tools used in studying molecular ecology. It explains genetic manipulation in terms of molecular ecology. The chapter explains the process of protein and DNA extraction from tissue samples. It also lists the non-PCR-based tools for studying genetic variations such as restriction fragment length polymorphism (RFLP). Moreover, the chapter discusses PCR-based molecular markers which may or may not require sequence information by referencing microsatellite loci and randomly amplified polymorphic DNA (RAPD) as respective examples. It notes the measurement of gene
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Erlich, Henry A. "Exclusions and Exonerations." In Genetic Reconstruction of the Past. Oxford University Press, 2023. http://dx.doi.org/10.1093/oso/9780197675366.003.0004.

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Abstract This chapter describes the first DNA exoneration, in which the wrongful conviction of Gary Dotson was overturned based on the results of the PCR-based HLA DQalpha test. Attempts to analyze the 11-year-old semen stain using restriction fragment length polymorphism (RFLP) technology had been unsuccessful. The author discusses several other HLA-DQalpha–based exonerations as well as many more recent cases using more recent DNA technology, the short tandem repeat (STR) markers. This chapter addresses the issues of mistaken eyewitness testimony, false confessions, and the reluctance of pros
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Budakva, Ye, K. Pochernyaev, S. Korinnyi, and M. Povod. "The use of mitochondrial genome polymorphism to establish pro-maternal breeds in the final hybrids of pigs." In Technological innovation engineering manufacturing agricultural complex and zoology (1st ed ). Primedia eLaunch LLC, 2020. http://dx.doi.org/10.36074/ti:emacaz.ed-1.03.

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The purpose of this work was to determine the origin of pigs (Large White × Landrace) × Maxgro of the final hybrid using mitochondrial DNA markers. As a genetic material used bristles from the auricle of commercial pigs (large white × landrace) × Maxgro. DNA secretion was carried out according to the method of Korinnyi S.M., etc.2005, using Chelex ion exchange resin – 100. For the analysis of the mitochondrial genome, the method of polymorphism of the lengths of restriction fragments amplified in PCR was used (Polymerase chain reaction-restriction fragment of polymorphism – PCR-RFLP). The D-lo
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Qiu Y., Chen Z., Tang N.L.-S., and Cheng J.C.-Y. "Promoter polymorphism of matrilin-1 gene (MATN1) is associated with susceptibility to adolescent idiopathic scoliosis: a casecontrol study." In Studies in Health Technology and Informatics. IOS Press, 2008. https://doi.org/10.3233/978-1-58603-888-5-325.

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Previous studies have shown genetic factor is important in adolescent idiopathic scoliosis (AIS). Matrilin-1 is a non-collagenous protein, which function in the organization of the extracellular matrix. Matrillin-1 mutant mice showed similar phenotypes to scoliosis. A recent study showed that MATN1 gene is a susceptibility region for AIS. Considering all above, Matn1 gene could be considered as a good candidate gene for AIS. For initial screening, 7 htSNPs were genotyped in 197 cases and 172 controls. Next, we confirmed the significant associations of the four SNPs in additional 223 cases and
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Conference papers on the topic "PCR-RFLP markers"

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Buriachenko, Semen, and Borys Stegniy. "VARIABILITY OF GENETIC MARKERS OF AVIAN INFLUENZA A OF H1N1 AND H7N9 STRAINS. EXPRESS-METHOD DIAGNOSTICS PCR-RFLP FIELD ISOLATES." In Relevant Issues of the Development of Science in Central and Eastern European Countries. Publishing House “Baltija Publishing”, 2019. http://dx.doi.org/10.30525/978-9934-588-11-2_1.

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Eltai, Nahla O., Sara H. Al-Hadidi, Asmaa A. Al Than, Sanjay H. Doiphode, and Hadi M. Yassine. "Salmonellosis among Pediatric Population in Qatar: Prevalence, Antibiotic Resistance and Molecular Epidemiology." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0126.

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Objectives: This study aims to characterize at the molecular level the genes encoding resistance in Salmonella and explain the molecular mechanisms underlying resistance to ceftriaxone, cefepime, amoxicillin-clavulanate, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, colistin and azithromycin in Salmonella. It aims as well to characterize the 16S rRNA gene region by restriction fragment length polymorphism (RFLP) to investigate if this region constitutes an appropriate ‘coincidental’ marker to distinguish important pathogenic Salmonella species. Finally, determine the lineages o
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Reports on the topic "PCR-RFLP markers"

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Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

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Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and be
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Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7695875.bard.

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High Grain Protein Content (GPC) is a desirable trait in breadmaking and pasta wheat varieties because of its positive effects on quality and nutritional value. However, selection for GPC is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. The long-term goal of this project is to provide a better understanding of the genes controlling GPC in wheat. The specific objectives of this project were: a) to develop a high-density genetic map of the GPC gene in tetraploid wheat, b) to construct a T. turgidum Bacterial Artificial Chromosome (BAC) libra
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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark
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Paran, Ilan, and Molly Jahn. Genetics and comparative molecular mapping of biochemical and morphological fruit characters in Capsicum. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7586545.bard.

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Original objectives: The overall goal of our work was to gain information regarding the genetic and molecular control of pathways leading to the production of secondary metabolites determining major fruit quality traits in pepper and to develop tools based on this information to assist in crop improvement. The specific objectives were to: (1) Generate a molecular map of pepper based on simple sequence repeat (SSR) markers. (2) Map QTL for capsaicinoid (pungency) content (3) Determine possible association between capsaicinoid and carotenoid content and structural genes for capsaicinoid and caro
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Wongpiyabovorn, Jongkonnee, Nattiya Hirankarn, Yingyos Avihingsanon, Tewin Tencomnao, Yong Poovorawan, and Kriangsak Ruchusatsawat. The association between immunogenetics and genetic susceptibility of psoriasis in Thai population. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.27.

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Psoriasis is T-cell-mediated skin autoimmunity, required environmental triggers and genetic susceptibility factors to become manifested. Psoriasis is a chronic skin disease characterized by the abnormal hyperproliferation and differentiation of the epidermis, elongated and prominent blood vessels and a thick perivascular lymphocytic infiltrate. Vascular endothelial growth factor (VEGF) gene play important role in pathogenesis of various diseases with angiogenic basis such as breast cancer and autoimmune disease including psoriasis. Many studies analyzed the association of VEGF gene polymorphis
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