Academic literature on the topic 'Plasmid production'

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Journal articles on the topic "Plasmid production"

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Trivedi, Ram Narayan, Parvez Akhtar, Jonathan Meade та ін. "High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacBby IntroducingincMutations". Applied and Environmental Microbiology 80, № 23 (2014): 7154–60. http://dx.doi.org/10.1128/aem.02445-14.

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ABSTRACTFor small-copy-number pUC-type plasmids, theinc1andinc2mutations, which deregulate replication, were previously found to increase the plasmid copy number 6- to 7-fold. Because plasmids can exert a growth burden, it was not clear if further amplification of copy number would occur due toincmutations when the starting point for plasmid copy number was orders of magnitude higher. To investigate further the effects of theincmutations and the possible limits of plasmid synthesis, the parent plasmid pNTC8485 was used as a starting point. It lacks an antibiotic resistance gene and has a copy
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Kojic, Milan, Ivana Strahinic, Djordje Fira, Branko Jovcic, and Ljubisa Topisirovic. "Plasmid content and bacteriocin production by five strains ofLactococcus lactisisolated from semi-hard homemade cheese." Canadian Journal of Microbiology 52, no. 11 (2006): 1110–20. http://dx.doi.org/10.1139/w06-072.

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In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains
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Münch, Karin M., Johannes Müller, Sarah Wienecke, et al. "Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity." Applied and Environmental Microbiology 81, no. 17 (2015): 5976–86. http://dx.doi.org/10.1128/aem.00807-15.

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ABSTRACTDuring the past 2 decades,Bacillus megateriumhas been systematically developed for the gram-per-liter scale production of recombinant proteins. The plasmid-based expression systems employed use a xylose-controlled promoter. Protein production analyses at the single-cell level using green fluorescent protein as a model product revealed cell culture heterogeneity characterized by a significant proportion of less productive bacteria. Due to the enormous size ofB. megaterium, such bistable behavior seen in subpopulations was readily analyzed by time lapse microscopy and flow cytometry. Cel
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Fong, Ryan, Zhihao Hu, C. Richard Hutchinson, Jianqiang Huang, Stanley Cohen, and Camilla Kao. "Characterization of a Large, Stable, High-Copy-Number Streptomyces Plasmid That Requires Stability and Transfer Functions for Heterologous Polyketide Overproduction." Applied and Environmental Microbiology 73, no. 4 (2006): 1296–307. http://dx.doi.org/10.1128/aem.01888-06.

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ABSTRACT A major limitation to improving small-molecule pharmaceutical production in streptomycetes is the inability of high-copy-number plasmids to tolerate large biosynthetic gene cluster inserts. A recent finding has overcome this barrier. In 2003, Hu et al. discovered a stable, high-copy-number, 81-kb plasmid that significantly elevated production of the polyketide precursor to the antibiotic erythromycin in a heterologous Streptomyces host (J. Ind. Microbiol. Biotechnol. 30:516-522, 2003). Here, we have identified mechanisms by which this SCP2*-derived plasmid achieves increased levels of
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Fridjonsson, Olafur, та Ralf Mattes. "Production of Recombinant α-Galactosidases inThermus thermophilus". Applied and Environmental Microbiology 67, № 9 (2001): 4192–98. http://dx.doi.org/10.1128/aem.67.9.4192-4198.2001.

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ABSTRACT A Thermus thermophilus selector strain for production of thermostable and thermoactive α-galactosidase was constructed. For this purpose, the native α-galactosidase gene (agaT) ofT. thermophilus TH125 was inactivated to prevent background activity. In our first attempt, insertional mutagenesis ofagaT by using a cassette carrying a kanamycin resistance gene led to bacterial inability to utilize melibiose (α-galactoside) and galactose as sole carbohydrate sources due to a polar effect of the insertional inactivation. A Gal+ phenotype was assumed to be essential for growth on melibiose.
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Chakrabarty, P. K., Sheo Raj, M. K. Meshram, A. Mahadevan, and D. W. Gabriel. "Plasmid-borne determinants of pigmentation, exopolysaccharide production, and virulence in Xanthomonas campestris pv. malvacearum." Canadian Journal of Microbiology 41, no. 8 (1995): 740–45. http://dx.doi.org/10.1139/m95-101.

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Three plasmids of 55.0, 31.2, and 7.4 kb, designated as pXCM18-I, pXCM18-II, and pXCM18-III, respectively, were identified and isolated from strain HR13B/2 of Xanthomonas campestris pv. malvacearum. The bacterium was cured of all three plasmids in high frequency (3.1%) with heat (42 °C). The cured strains were nonmucoid and light yellow and were greatly impaired in exopolysaccharide production and virulence. Transformation of a cured strain using a mixture of purified plasmid DNA from HR13B/2 resulted in colonies carrying all six possible combinations of the three plasmids. Of the transformant
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McMillan, Elizabeth A., Jamie L. Wasilenko, Kaitlin A. Tagg, et al. "Carriage and Gene Content Variability of the pESI-Like Plasmid Associated with Salmonella Infantis Recently Established in United States Poultry Production." Genes 11, no. 12 (2020): 1516. http://dx.doi.org/10.3390/genes11121516.

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Salmonella Infantis carrying extended spectrum β-lactamase blaCTX-M-65 on a pESI-like megaplasmid has recently emerged in United States poultry. In order to determine the carriage rate and gene content variability of this plasmid in U.S. Salmonella Infantis, whole genome sequences of Salmonella isolates from humans and animals in the U.S. and internationally containing the pESI-like plasmid were analyzed. The U.S. Department of Agriculture Food Safety and Inspection Service (FSIS) identified 654 product sampling isolates containing pESI-like plasmids through hazard analysis and critical contro
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Ishibashi, Y. "Genetic Studies into Musty Odor Production by Actinomycetes." Water Science and Technology 25, no. 2 (1992): 171–76. http://dx.doi.org/10.2166/wst.1992.0049.

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Musty taste and odor is a serious problem for drinking water. Much data regarding its occurrence are being accumulated. However, we may be able to solve this problem by inquiring into the nature of secondary metabolism through the mevalonic acid pathway in actinomycetes and/or cyanobacteria that yield musty smelling compounds. Therefore, genetic analysis is applied to look for new solutions. By investigating Streptomyces sp. with the protocol to recover plasmid DNA, it was difficult to get the infinitesimally small covalently closed circular (ccc) plasmid. On the other hand, Streptomyces strai
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Hausjell, Johanna, Regina Kutscha, Jeannine D. Gesson, Daniela Reinisch, and Oliver Spadiut. "The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System." Bioengineering 7, no. 1 (2020): 8. http://dx.doi.org/10.3390/bioengineering7010008.

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Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host Escherichia coli presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid loss over prolonged cultivations. To improve production, efforts are made to establish plasmid-free expression, ensuring more stable process conditions. Another strategy to stabilize production processes is lactose induction, leading to increased soluble product formation and cell fitness, as shown
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Carnes,, Aaron E. "High-Yield Plasmid DNA Production." Genetic Engineering & Biotechnology News 32, no. 8 (2012): 42–43. http://dx.doi.org/10.1089/gen.32.8.18.

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Dissertations / Theses on the topic "Plasmid production"

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O'Mahony, Kevin. "Large scale plasmid production /." [S.l.] : [s.n.], 2005. http://library.epfl.ch/theses/?nr=3320.

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Yap, Wee Ching Melvyn. "Analysis of retroviral production in murine leukaemia virus." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325497.

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Bower, Diana M. (Diana Morgan). "Development of new tools for the production of plasmid DNA biopharmaceuticals." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/76475.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2012.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 96-104).<br>DNA vaccines and gene therapies that use plasmid DNA (pDNA) as a vector have gained attention in recent years for their good safety profile, ease of manufacturing, and potential to treat a host of diseases. With this interest comes increased demand for high-yield manufacturing processes. Overall, this thesis aims to develop new, innovative tools for the production of plasmid DNA biopharmaceuticals. As one pa
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Hales, Barbara A. "Plasmid determined fimbrial production responsible for bacterial colonization of the urinary tract." Thesis, University of Edinburgh, 1985. http://hdl.handle.net/1842/23962.

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Cheng, Chinyuan. "Engineering strategies to optimize plasmid stability and protein production in recombinant Saccharomyces cerevisiae fermentation /." The Ohio State University, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487779914824623.

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Chamsart, Saedthawat. "A cell lysis reactor for the production of plasmid DNA from recombinant E.coli for gene therapy." Thesis, University of Birmingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366017.

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Pensri, Charoensit. "Development of inhibition methods for pro-inflammatory cytokine production induced by cationic carrier/plasmid DNA complex." 京都大学 (Kyoto University), 2009. http://hdl.handle.net/2433/126605.

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Robinson, Susan Clare. "Enhanced production of a recombinant, thermostable alpha-amylase in Streptomyces lividans : effects of plasmid construction and culture conditions." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393582.

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García, Mark Megan Olga. "Production and validation of anti-HCV antibodies for viral neutralization." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278578.

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Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. The objective of this master’s thesis project is to produce human antibody (IgG) against HCV. This project will focus on the monoclonal antibodies (mAbs) HEPC3, AR3C, HEPC74, and HCV1. These four antibodies have been isolated from patients who have successfully cleared the infection, and their sequences and structu
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Andersson, Christin. "Production and delivery of recombinant subunit vaccines." Doctoral thesis, KTH, Biotechnology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3027.

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<p>Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. In addition, different strategies fordelivery of subunit vaccines by RNA or DNA immunization havebeen investigated.</p><p>Attempts to create general production strategies forrecombinant protein immunogens in such a way that these areadapted for association with an adjuvant formulation wer
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Books on the topic "Plasmid production"

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Perinot, Glen. Genomic analysis of human papillomavirus types 16 and 18: Production of recombinant plasmid : part I in the production of a recombinant vaccine for human papillomavirus. Laurentian University, 1993.

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PPLA 2003, Messina and Catania, Italy, 18-19 September 2003. Plasma Production by Laser Ablation: PPLA 2003. World Scientific Publishing, 2005.

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Bertolini, Joseph, Neil Goss, and J. M. Curling. Production of plasma proteins for therapeutic use. John Wiley & Sons, 2013.

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Bertolini, Joseph, Neil Goss, and John Curling, eds. Production of Plasma Proteins for Therapeutic Use. John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118356807.

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Cham, Yuen-wai. A study of digital printing in plastic card production. LCP, 1999.

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Formulation, &. Production Conference (1996 Indianapolis Ind ). Powder coating: Formulation & Production Conference : Tuesday, September 17, 1996, Westin Hotel, Indianapolis, IN : conference proceedings. The Institute, 1996.

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Bogenschütz, August Friedrich. Analysis and testing in production of circuit boards and plated plastics. Finishing Publications, 1985.

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Jalufka, N. W. Laser production and heating of plasma for MHD application. National Aeronautics and Space Administration, Scientific and Technical Information Division, 1988.

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Jalufka, N. W. Laser production and heating of plasma for MHD application. National Aeronautics and Space Administration, Scientific and Technical Information Division, 1988.

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Schoukens, A. F. S. The production of refined ferromanganese in a transferred-arc plasma furnace. Council for Mineral Technology, 1988.

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Book chapters on the topic "Plasmid production"

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Shoda, Makoto. "Surfactin Production and Plasmid Stability." In Biocontrol of Plant Diseases by Bacillus subtilis. CRC Press, 2019. http://dx.doi.org/10.1201/9780429027635-8.

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Brand, Eva, Kathrin Ralla, and Peter Neubauer. "Strategies for Plasmid DNA Production inEscherichia coli." In Biopharmaceutical Production Technology. Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527653096.ch1.

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Lara, Alvaro R., and Octavio T. Ramírez. "Plasmid DNA Production for Therapeutic Applications." In Recombinant Gene Expression. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-433-9_14.

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Lara, Alvaro R., and Octavio T. Ramírez. "Plasmid DNA Production for Therapeutic Applications." In Recombinant Gene Expression. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-433-9_35.

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Schmeer, Marco, and Martin Schleef. "Production of Plasmid DNA as Pharmaceutical." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2727-2_17.

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Schleef, Martin, and Markus Blaesen. "Production of Plasmid DNA as Pharmaceutical." In Gene Therapy of Cancer. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-561-9_25.

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Hanak, Julian A. J., and Rocky M. Cranenburgh. "Antibiotic-Free Plasmid Selection and Maintenance in Bacteria." In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9749-4_9.

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Kelly, Michael D., and Joel E. Mortensen. "A Low-Copy Number Plasmid Mediating β-Lactamase Production by Xanthomonas Maltophilia." In Antimicrobial Resistance. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-9203-4_6.

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Mairhofer, Juergen, and Alvaro R. Lara. "Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0345-0_38.

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Hughes, Stephen R., Tauseef R. Butt, Scott Bartolett, and Steven B. Riedmuller. "Automated Systems of Plasmid-Based Functional Proteomics to Improve Microbes for Biofuel Production." In Microbiology Monographs. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-21467-7_11.

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Conference papers on the topic "Plasmid production"

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Zhang, Xue-Qing, Mark Chen, Robert Lam, Xiaoyang Xu, Eiji Osawa, and Dean Ho. "A Platform Approach to Gene Delivery via Surface Modified Nanodiamonds." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13340.

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The purpose of gene therapy is to introduce foreign genetic material into host cells to either supplement aberrant genes or to endow additional biological functions. To date, however, there has been only modest progress towards this goal, mainly due to the lack of safe, effective and broadly applicable delivery methods. Functional nanodiamonds (NDs) are rapidly emerging as promising platform carriers for next-generation therapeutics due to their innate biocompatibility, scalability, precise particle distribution, high surface area-to-volume ratio, near-spherical aspect ratio, and easily adapta
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Amoretti, M. "Non-Destructive Positron Plasma Diagnostics for Antihydrogen Production." In NON-NEUTRAL PLASMA PHYSICS V: Workshop on Non-Neutral Plasmas. AIP, 2003. http://dx.doi.org/10.1063/1.1635167.

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Higaki, H., Y. Enomoto, N. Kuroda, et al. "Towards the production of anti-hydrogen beams." In NON-NEUTRAL PLASMA PHYSICS VIII: 10th International Workshop on Non-Neutral Plasmas. AIP, 2013. http://dx.doi.org/10.1063/1.4796069.

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Baumer, Stefan M. B., Wim A. G. Timmers, Mark Krichever, and Vladimir Gurevich. "Temperature-compensated plastic lens for visible light." In Optical Systems Design and Production, edited by Fritz Merkle. SPIE, 1999. http://dx.doi.org/10.1117/12.360028.

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Fukumasa, Osamu. "Isotope Effect of H−/D− Volume Production in Low-Pressure H2/D2 Plasmas — Negative Ion Densities versus Plasma Parameters." In PRODUCTION AND NEUTRALIZATION OF NEGATIVE IONS AND BEAMS: 10th International Symposium on Production and Neutralization of Negative Ions and Beams. AIP, 2005. http://dx.doi.org/10.1063/1.1908283.

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PANDEY, UMESH, JAN ARILD STORMYR, ALIREZA HASSANI, RAJAN JAISWAL, HILDEGUNN H. HAUGEN, and BRITT M. E. MOLDESTAD. "PYROLYSIS OF PLASTIC WASTE TO ENVIRONMENTALLY FRIENDLY PRODUCTS." In ENERGY PRODUCTION AND MANAGEMENT 2020. WIT Press, 2020. http://dx.doi.org/10.2495/epm200071.

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Vallinga, P. M., D. C. Schram, and H. J. Hopman. "Plasma neutralizers." In Production and neutralization of negative ions and beams. AIP, 1990. http://dx.doi.org/10.1063/1.39644.

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Kuroda, N., Y. Nagata, H. A. Torii, et al. "Radial compression of antiproton cloud for production of ultraslow antiproton beams." In NON-NEUTRAL PLASMA PHYSICS VII: Workshop on Non-Neutral Plasmas 2008. AIP, 2009. http://dx.doi.org/10.1063/1.3122278.

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Fauchais, P., J. F. Coudert, and B. Pateyron. "Production of Thermal plasmas." In Proceedings of the International School of Plasma Physics “Piero Caldirola”. WORLD SCIENTIFIC, 1996. http://dx.doi.org/10.1142/9789814447171_0001.

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El-Fayoumi, Amr Mohamed, Karim S. Zaki, and Ahmed S. Abou-Sayed. "3D Hydraulic Fracture Simulation for Injection in Plastic Shales." In SPE Production and Operations Symposium. Society of Petroleum Engineers, 2011. http://dx.doi.org/10.2118/142263-ms.

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Reports on the topic "Plasmid production"

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O'Connell, Caolionn L., and Phys Dept /Stanford U. Plasma Production via Field Ionization. Stanford Linear Accelerator Center (SLAC), 2005. http://dx.doi.org/10.2172/878428.

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Tsidulko, Yu A., and H. L. Berk. Plasma analog of particle-pair production. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/392841.

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Coensgen, F. H., A. H. Futch, and A. W. Molvik. Linear plasma-based tritium production facility. Office of Scientific and Technical Information (OSTI), 1989. http://dx.doi.org/10.2172/6338201.

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Nair, Ajay, and Brandon H. Carpenter. Sustainable Plastic Mulch Options for Vegetable Production Systems. Iowa State University, Digital Repository, 2014. http://dx.doi.org/10.31274/farmprogressreports-180814-451.

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Nair, Ajay, and Bernard J. Havlovic. Colored Plastic Mulches for High Tunnel Tomato Production. Iowa State University, Digital Repository, 2014. http://dx.doi.org/10.31274/farmprogressreports-180814-670.

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Sears, J. W. Plasma quench production of titanium from titanium tetrachloride. Office of Scientific and Technical Information (OSTI), 1994. http://dx.doi.org/10.2172/116695.

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Trow, J. R. H/sup -/ production in a multicusp microwave plasma. Office of Scientific and Technical Information (OSTI), 1985. http://dx.doi.org/10.2172/5638985.

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Author, Not Given. Plasma Carbothermic Reduction for Boron-Based Chemical Hydride Production. Office of Scientific and Technical Information (OSTI), 2011. http://dx.doi.org/10.2172/1003730.

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R. A. Cordes and A. Donaldson. Titanium Metal Powder Production by the Plasma Quench Process. Office of Scientific and Technical Information (OSTI), 2000. http://dx.doi.org/10.2172/765301.

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Author, Not Given. Modular Hybrid Plasma Systems for Low Cost Production of Nanoparticles. Office of Scientific and Technical Information (OSTI), 2009. http://dx.doi.org/10.2172/964934.

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