Relevant bibliographies by topics / Plastidial 16S rRNA gene

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Plastidial 16S rRNA gene'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Plastidial 16S rRNA gene"

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Sarah, Romac, F. Stern Rowena, Mahdi Bendif El, et al. "PhytoREF: a reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy." Molecular Ecology Resources 15, no. 6 (2015): 1435–45. https://doi.org/10.1111/1755-0998.12401.

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Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic ecosystems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morphological characters and have complex life cycles. To interpret environmental sequences, metabarcoding necessarily relies on taxonomically curated databases containing reference sequences of the targeted gene (or barcode) from identified organisms. To date, no such reference framework
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Decelle, Johan, Sarah Romac, Rowena F. Stern, et al. "PhytoREF: a reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy." Molecular Ecology Resources 15, no. 6 (2015): 1435–45. http://dx.doi.org/10.1111/1755-0998.12401.

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Beumer, Amy, and Jayne B. Robinson. "A Broad-Host-Range, Generalized Transducing Phage (SN-T) Acquires 16S rRNA Genes from Different Genera of Bacteria." Applied and Environmental Microbiology 71, no. 12 (2005): 8301–4. http://dx.doi.org/10.1128/aem.71.12.8301-8304.2005.

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ABSTRACT Genomic analysis has revealed heterogeneity among bacterial 16S rRNA gene sequences within a single species; yet the cause(s) remains uncertain. Generalized transducing bacteriophages have recently gained recognition for their abundance as well as their ability to affect lateral gene transfer and to harbor bacterial 16S rRNA gene sequences. Here, we demonstrate the ability of broad-host-range, generalized transducing phages to acquire 16S rRNA genes and gene sequences. Using PCR and primers specific to conserved regions of the 16S rRNA gene, we have found that generalized transducing
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Akihary, Claudia Valleria, and Beivy Jonathan Kolondam. "PEMANFAATAN GEN 16S rRNA SEBAGAI PERANGKAT IDENTIFIKASI BAKTERI UNTUK PENELITIAN-PENELITIAN DI INDONESIA." PHARMACON 9, no. 1 (2020): 16. http://dx.doi.org/10.35799/pha.9.2020.27405.

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ABSTRACTThe 16S rRNA gene has hyper variable region and different for one bacterial species to another. The gene is being used as research tool to help for accurate identification of bacteria in many fields in Indonesia. As a useful tool, the 16S rRNA gene sequence is important as to explore the potencies of a bacterial species. Sequencing of this gene is very useful for research in clinical study, fisheries, marine science, agricultural science, and animal husbandry in Indonesia. Keywords: 16S rRNA gene, research tool, bacteria, Indonesia ABSTRAKGen 16S rRNA memiliki region yang sangat bervar
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Dewhirst, Floyd E., Zeli Shen, Michael S. Scimeca, et al. "Discordant 16S and 23S rRNA Gene Phylogenies for the Genus Helicobacter: Implications for Phylogenetic Inference and Systematics." Journal of Bacteriology 187, no. 17 (2005): 6106–18. http://dx.doi.org/10.1128/jb.187.17.6106-6118.2005.

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ABSTRACT Analysis of 16S rRNA gene sequences has become the primary method for determining prokaryotic phylogeny. Phylogeny is currently the basis for prokaryotic systematics. Therefore, the validity of 16S rRNA gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. Discrepancies between 16S rRNA gene analyses and DNA-DNA hybridization and phenotypic analyses have been noted in the genus Helicobacter. To clarify these discrepancies, we sequenced the 23S rRNA genes for 55 helicobacter strains representing 41 taxa (>2,700 bases per sequence). Phylogenetic-t
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Chalker, Victoria J., and Joe Brownlie. "Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison." International Journal of Systematic and Evolutionary Microbiology 54, no. 2 (2004): 537–42. http://dx.doi.org/10.1099/ijs.0.02869-0.

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The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S rRNA intergenic spacer (IGS) region sequences. The nucleotide sequences of the 16S rRNA gene of two untyped mycoplasmas and the IGS region of 11 Mycoplasma species were determined and used for phylogenetic analysis. The two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published mycoplasma 16S rRNA gene sequences.
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Dingman, Douglas W. "Characterization ofPaenibacillus popilliaerRNA operons." Canadian Journal of Microbiology 50, no. 10 (2004): 779–91. http://dx.doi.org/10.1139/w04-068.

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The terminal 39 nucleotides on the 3' end of the 16S rRNA gene, along with the complete DNA sequences of the 5S rRNA, 23S rRNA, tRNAIle, and tRNAAlagenes were determined for Paenibacillus popilliae using strains NRRL B-2309 and Dutky 1. Southern hybridization analysis with a 16S rDNA hybridization probe and restriction-digested genomic DNA demonstrated 8 copies of the 16S rRNA gene in P. popilliae strains KLN 3 and Dutky 1. Additionally, the 23S rRNA gene in P. popilliae strains NRRL B-2309, KLN 3, and Dutky 1 was shown by I-CeuI digestion and pulsed-field gel electrophoresis of genomic DNA to
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Paul, Bobby. "Concatenated 16S rRNA sequence analysis improves bacterial taxonomy." F1000Research 11 (September 1, 2023): 1530. http://dx.doi.org/10.12688/f1000research.128320.3.

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Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations. Advances in DNA sequencing and bioinformatics workflows have facilitated sophisticated genome-based methods for microbial taxonomy although sequencing of the 16S rRNA gene is widely employed to identify and classify bacterial communities as a cost-effective and single-gene approach. However, the 16S rRNA sequence-based species identification accuracy is limited because of the occurrence of multiple copies of the 16S rRNA gene and higher sequence id
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Paul, Bobby. "Concatenated 16S rRNA sequence analysis improves bacterial taxonomy." F1000Research 11 (April 3, 2023): 1530. http://dx.doi.org/10.12688/f1000research.128320.2.

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Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations. Advances in DNA sequencing and bioinformatics workflows have facilitated sophisticated genome-based methods for microbial taxonomy although sequencing of the 16S rRNA gene is widely employed to identify and classify bacterial communities as a cost-effective and single-gene approach. However, the 16S rRNA sequence-based species identification accuracy is limited because of the occurrence of multiple copies of the 16S rRNA gene and higher sequence id
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Huang, Y., G. W. Stemke, and J. A. Robertson. "An unusual rRNA gene organization in Mycoplasma fermentans (incognitus strain)." Canadian Journal of Microbiology 41, no. 4-5 (1995): 424–27. http://dx.doi.org/10.1139/m95-056.

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The macro-restriction map of Mycoplasma fermentans (incognitus strain) was constructed and its rRNA genes were located on the map. It was found that this organism contains two sets of rRNA genes. The 16S and 23S rRNA genes were closely linked as two clusters. However, both 5S rRNA genes were separated from the 16S and 23S genes. The two 16S–23S rRNA gene clusters were arranged in an unusual tail to tail orientation.Key words: physical map, rRNA, Mycoplasma fermentans, genome, gene organization.
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Dissertations / Theses on the topic "Plastidial 16S rRNA gene"

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Silveira, Érico Leandro da [UNESP]. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA." Universidade Estadual Paulista (UNESP), 2004. http://hdl.handle.net/11449/94868.

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Made available in DSpace on 2014-06-11T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-04-07Bitstream added on 2014-06-13T19:14:40Z : No. of bitstreams: 1 silveira_el_me_jabo.pdf: 655008 bytes, checksum: ac46de019a2703894b3c1bc37633b43c (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da con
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Silveira, Érico Leandro da. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA /." Jaboticabal : [s.n.], 2004. http://hdl.handle.net/11449/94868.

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Resumo: Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da construção de bibliotecas metagenômicas, o que constitui uma poderosa abordagem para explorar a diversidade microbiana de solo fornecendo inclusive dados sobre os microrganismos não cultiváveis desse habitat. Este trabalho teve por objetivo comparar e estimar a diversidade de comunidades bacterianas, em solos de duas áreas, send
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Pereira, Juliana Vianna. "Comparação por análise molecular da diversidade bacteriana da saliva de pacientes com diferentes índices de higiene bucal." Universidade Federal do Amazonas, 2009. http://tede.ufam.edu.br/handle/tede/3064.

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Made available in DSpace on 2015-04-20T12:31:09Z (GMT). No. of bitstreams: 1 Juliana Vianna Pereira.pdf: 4570056 bytes, checksum: c660fca0452deacfa10d23ee9bc79a2b (MD5) Previous issue date: 2009-08-26<br>The complex microbiota of the oral cavity has been intensively studied and saliva is characterized by microorganisms which colonize different regions of mouth, such as tongue, supragengival and subgingival biofilm. Considering this, the purpose of this study was to evaluate the bacterial diversity of the saliva of patients with different levels of oral hygiene according to the Silness,
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Grešová, Katarína. "Klasifikace bakterií do taxonomických kategorií na základě vlastností 16s rRNA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2020. http://www.nusl.cz/ntk/nusl-417246.

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The main goal of this thesis was to design and implement a tool that would be able to classify the sequences of the 16S rRNA gene into taxonomic categories using the properties of the 16S rRNA gene. The created tool analyzes all input sequences simultaneously, which differs from common classification approaches, which classify input sequences individually. This tool relies on the fact that bacteria contain several copies of the 16S rRNA gene, which may differ in sequence. The main contribution of this work is design, implementation and evaluation of the capabilities of this tool. Experiments h
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Kim, Min Seok. "AN INTEGRATED INVESTIGATION OF RUMINAL MICROBIAL COMMUNITIES USING 16S rRNA GENE-BASED TECHNIQUES." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316383870.

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Oliveira, Rosangela Claret de. "Isolamento de ureaplasma e micoplasma do trato reprodutivo de ovinos e caprinos e tipificação genotípica por meio da PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-12012009-105144/.

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Micoplasmas e ureaplasmas têm sido isolados de pequenos ruminantes, mas existem poucos estudos. O Mycoplasma ovine/caprine sorotipo 11, conhecido como cepa 2D, ainda não classificado como espécie, vem sendo relacionado a casos de vulvovaginite e problemas reprodutivos em ovinos e caprinos. Os ureaplasmas apresentam a habilidade em induzirem doenças no trato genital, confirmada por inoculações experimentais de isolados obtidos de animais doentes em animais saudáveis, resultando em moderada granularidade e hiperemia na vulva. Estes ureaplasmas não receberam ainda a designação de espécie, sendo a
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De, Moors Anick. "Comparison of gene organization in the region that surrounds the 16S rRNA gene in seven different Sulfolobales." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32533.pdf.

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Thorson, Mary Leah. "Characterization of the structure and function of a Bacteroides thetaiotaomicron 16S rRNA promoter." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/42962.

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The bacteroides group is a subdivision in the <I>Cytophaga-Flavobacterium-Bacteroides</I> phylum. This group is as phylogenetically distinct from other Gram-negative enterics, including <I>Escherichia coli</I>, as they are from Gram-positive organisms. Furthermore, there is no cross expression between genes of <I>E. coli</I> and <I>Bacteroides</I> species. It is thought that this difference in gene expression lies in part at the level of transcription initiation and is due to the sequences within the promoter region itself. A putative consensus sequence for <I>Bacteroides</I> promoters has
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Kam, Sin-yee. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971052.

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Aiemsum-ang, Porntipa. "Isolation, Systematics and Screening of Members of the Streptomyces violaceusniger 16S rRNA Gene Clade." Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506611.

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Books on the topic "Plastidial 16S rRNA gene"

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Wieland, Heidemarie. Characterization of a Haloarchaeal 16s Rrna Gene Clone Library from Alpine Rock Salt from Bad Ischl, Austri. GRIN Verlag GmbH, 2007.

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Goldenberger, Daniel. Detection of bacterial pathogens in clinical specimens by broad-range PCR amplification and direct sequencing of part of the 16S rRNA gene. 1997.

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Kirchman, David L. Community structure of microbes in natural environments. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0004.

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Community structure refers to the taxonomic types of microbes and their relative abundance in an environment. This chapter focuses on bacteria with a few words about fungi; protists and viruses are discussed in Chapters 9 and 10. Traditional methods for identifying microbes rely on biochemical testing of phenotype observable in the laboratory. Even for cultivated microbes and larger organisms, the traditional, phenotype approach has been replaced by comparing sequences of specific genes, those for 16S rRNA (archaea and bacteria) or 18S rRNA (microbial eukaryotes). Cultivation-independent appro
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Kirchman, David L. Predation and protists. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0009.

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Protists are involved in many ecological roles in natural environments, including primary production, herbivory and carnivory, and parasitism. Microbial ecologists have been interested in these single-cell eukaryotes since Antonie van Leeuwenhoek saw them in his stool and scum from his teeth. This chapter focuses on the role of protozoa (purely heterotrophic protists) and other protists in grazing on other microbes. Heterotrophic nanoflagellates, 3–5 microns long, are the most important grazers of bacteria and small phytoplankton in aquatic environments. In soils, flagellates are also importan
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Kirchman, David L. Processes in anoxic environments. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0011.

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During organic material degradation in oxic environments, electrons from organic material, the electron donor, are transferred to oxygen, the electron acceptor, during aerobic respiration. Other compounds, such as nitrate, iron, sulfate, and carbon dioxide, take the place of oxygen during anaerobic respiration in anoxic environments. The order in which these compounds are used by bacteria and archaea (only a few eukaryotes are capable of anaerobic respiration) is set by thermodynamics. However, concentrations and chemical state also determine the relative importance of electron acceptors in or
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Book chapters on the topic "Plastidial 16S rRNA gene"

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Hall, Michael, and Robert G. Beiko. "16S rRNA Gene Analysis with QIIME2." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8728-3_8.

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Einarsson, Gisli G., and Sébastien Boutin. "Techniques: culture, identification and 16S rRNA gene sequencing." In The Lung Microbiome. European Respiratory Society, 2019. http://dx.doi.org/10.1183/2312508x.10000819.

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Pimenta, Mélanie, Margaux Gaschet, and Sylvain Meyer. "Targeted 16S rRNA Gene Sequencing for Water Samples." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4726-4_6.

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Bally, René, Jacqueline Haurat, and Philippe Normand. "Azospirillum Phylogeny Based on rrs (16S rRNA Gene) Sequences." In Azospirillum VI and Related Microorganisms. Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79906-8_12.

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Miyaue, Noriyuki. "16S rRNA Gene Amplicon Analysis of Human Gut Microbiota." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3682-4_35.

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Huang, Yu-An, Zhi-An Huang, Zhu-Hong You, et al. "Precise Prediction of Pathogenic Microorganisms Using 16S rRNA Gene Sequences." In Intelligent Computing Theories and Application. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-26969-2_13.

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Comtet-Marre, Sophie, Oshma Chakoory, and Pierre Peyret. "Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis." In Microbial Environmental Genomics (MEG). Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2871-3_10.

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Fantini, Elio, Giulio Gianese, Giovanni Giuliano, and Alessia Fiore. "Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-1720-4_5.

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Duduk, Bojan, Samanta Paltrinieri, Ing-Ming Lee, and Assunta Bertaccini. "Nested PCR and RFLP Analysis Based on the 16S rRNA Gene." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_14.

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Singleton, David R., Steven L. Rathbun, Glen E. Dyszynski, and William B. Whitman. "Section 7 update: LIBSHUFF Comparisons of 16S rRNA Gene Clone Libraries." In Molecular Microbial Ecology Manual. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_703.

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Conference papers on the topic "Plastidial 16S rRNA gene"

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Demeter, Marc, Shawna Johnston, Kim Dockens, and Raymond J. Turner. "Molecular MIC Diagnoses from ATP Field Test: Streamlined Workflow from Field to 16S rRNA Gene Metagenomics Results." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09420.

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Abstract Microbiologically influenced corrosion (MIC) describes the negative impacts of microbial growth within various industries. While culture based growth tests are the traditional means of assessing MIC, they are not accurate. New culture-independent assays have been developed; one of which is the adenosine triphosphate (ATP) assay. This field assay allows for indirect enumeration of microorganisms; however, it does not divulge which microorganisms are present. Molecular methods such as 16S metagenomics (16S rRNA gene sequencing and data interpretation) are often used for characterizing t
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Manna, Kathleen, Joseph Moore, Kenneth Wunch, et al. "Relative Performance of Various 16S iTag Amplicon Sequencing Primer Pairs in Profiling Microbial Communities Relevant to Oil & Gas Operations." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13442.

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Abstract The oil and gas industry's efforts to characterize microbial communities in oilfield process fluids has shed increasing light on the influence of bacteria and archaea on hydrocarbon production. Notably, topside or downhole water contamination by microorganisms such as sulfate-reducing bacteria, acid-producing bacteria, and/or other halophiles can negatively impact asset integrity and reduce the quality and quantity of produced hydrocarbons. Molecular microbiology methods have contributed to our understanding of these complex processes by providing unprecedented resolution of resident
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Al-Humam, Abdulmohsen A., Tony Y. Rizk, Jan A. Sunner, and Iwona B. Beech. "Effects of Nitrate on Bacterial Communities in an Oil Field Environment." In CORROSION 2010. NACE International, 2010. https://doi.org/10.5006/c2010-10249.

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Abstract The study aimed to determine whether nitrate-utilizing sulfate-reducing bacteria (SRB) thrive in the Hawtah oil field produced water re-injection system (PWRI). Bacterial populations were recovered through enrichments in selective growth media from samples collected at different locations in PWRI. Characterization of purified Hawtah field SRB isolates using 16S rRNA gene-based phylogeny, revealed the presence of an isolate, coded DD-H SRB, showing a high 16S rRNA sequence similarity (99%) with Desulfovibrio desulfuricans spp. desulfuricans. The change from sulfate to nitrate reduction
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Alabbas, Faisal M., Anthony E. Kakpovbia, John R. Spear, Brajendra Mishra, and David L. Olson. "Utilization of 454 Pyrosequencing of 16S rRNA for Biodiversity Investigations of Crude Oil Systems." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-3827.

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Abstract Microbiologically influenced corrosion (MIC) is a major problem that impacts crude oil production, transportation and storage infrastructures. Indigenous microorganisms that naturally reside in oil reservoirs are able to induce localized changes in the aqueous environment and lead to catastrophic damages. The study herein applies molecular techniques to investigate the microbial communities associated with corrosion products collected from crude oil pipelines. Small subunit ribosomal rRNA gene pyrosequencing was used to identify microbial communities present in each system. The result
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Wrangham, Jodi B., Joseph E. Penkala, Seth D’Imperio, Brent M. Peyton, and Kenneth G. Wunch. "Utilization of a 16S rRNA Gene Microarray to Analyze the Efficacy of Oil and Gas Industry Bacteria Culture Media." In CORROSION 2010. NACE International, 2010. https://doi.org/10.5006/c2010-10408.

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Abstract It is widely recognized that bacteria and archae are frequently contained within production fluids and can cause numerous problems and cost countless dollars to the oil and gas industries. Current techniques commonly employed to detect and enumerate these microorganisms and to test the efficacy of microbiocides focus on serial dilution culture methods. Unfortunately, culture-dependent methods allow for the growth of only a fraction of the field population present and do not support the growth of numerous potentially significant species. Currently, it is estimated that less than 15% of
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Romero, J. M., M. Amaya, and L. Martinez. "Study of Microbial Consortia Associated to Corrosion in Seawater Injection Systems." In CORROSION 2001. NACE International, 2001. https://doi.org/10.5006/c2001-01244.

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Abstract This paper is a study dedicated to identify aerobic bacteria, which influence MIC phenomena in pipelines used in a seawater injection system in the Gulf of Mexico. Fifteen aerobic bacterial strains were isolated from a bioprobe exposed during 45 days in a seawater pipeline. Three bacterial strains named IMP-M1, IMP-M5 and IMP-M9 were analyzed by 16S rRNA gene sequence analysis. Three different available gene databases were consulted to perform the phylogenetic characterization. The sequence analysis shows that Vibrio hollisae is the closest match to the IMP-M1 strain however with a 5.
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Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor
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McCulloch, Iain, Michael Whitman, Danika Nicoletti, Greg Howard, and Neil Sharma. "Validation of an Optimized qPCR Workflow for MIC Risk Identification and Oilfield Microbial Monitoring." In MECC 2023. AMPP, 2023. https://doi.org/10.5006/mecc2023-20130.

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In this work, a scalable workflow for field sample preservation, DNA extraction, and quantitative polymerase chain reaction (qPCR) was developed and validated for accurate and rapid oilfield microbial monitoring and microbiologically influenced corrosion (MIC) risk identification. Validation experiments were performed on a variety of challenging oilfield sample types including produced water and pigging sludge to assess the complete optimized qPCR workflow and eight MIC-related qPCR targets including sulfate reducing prokaryotes (SRP) and corrosive methanogens (micH). The predicted in silico t
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Leach, David G., Wei Wang, Chao Yan, Dillon Mattis, Ron MacLeod, and Wei Wei. "Molecular Deep Dive into Oilfield Microbiologically Influenced Corrosion: a Detailed Case Study of MIC Failure Analysis in an Unconventional Asset." In CONFERENCE 2022. AMPP, 2022. https://doi.org/10.5006/c2022-17948.

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Abstract This work details a microbiologically influenced corrosion (MIC) failure analysis case study for a produced water pipeline. A pipeline in a shale and tight asset experienced heavy corrosion and ultimate failure within a 7-month period, with estimated corrosion rate at 161 mils per year (MPY), or 4.1 mm per year (MMPY). Upon removal by the inspection team, heavy white deposit buildup (a suspected microbial biofilm) was observed directly associated with the corrosion failure on top of a black scale underlayer. Detailed assessments were performed using ATP photometry, qPCR speciation, an
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Alabbas, Faisal M., Anthony Kakpovbia, Brajendra Mishra, Charles Williamson, John R. Spear, and David L. Olson. "Corrosion of Linepipe Carbon Steel (X52) Influenced by a SRB Consortium Isolated from a Sour Oil Well." In CORROSION 2013. NACE International, 2013. https://doi.org/10.5006/c2013-02275.

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Abstract This work investigates microbiologically influenced corrosion (MIC) of API(1) 5L X52 linepipe steel by a sulfate-reducing bacteria (SRB) consortium. The SRB consortium used in this study was cultivated from a sour oil well in Louisiana, USA. 16S rRNA gene sequence analysis indicated that the mixed bacterial consortium contained three phylotypes: members of the Proteobacteria (Desulfomicrobium sp.), Firmicutes (Clostridium sp.) and Bacteroidetes (Anaerophaga sp.). The biofilm and pit morphology that developed with time were characterized with field emission scanning electron microscopy
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Reports on the topic "Plastidial 16S rRNA gene"

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ธนาศุภวัฒน์, สมบูรณ์, та คณิต สุวรรณบริรักษ์. อนุกรมวิธานและฤทธิ์ต้านจุลชีพของแอคติโนมัยสีทจากดินป่าพรุในจังหวัดระยอง : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2014. https://doi.org/10.58837/chula.res.2014.22.

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การศึกษาอนุกรมวิธานของแอคติโนมัยสีททั้งหมด 77 ไอโซเลตที่คัดแยกได้จากตัวอย่างดินป่าพรุ่ในจังหวัดระยองจำนวน 16 ตัวอย่าง พบว่าแอคติโนมัยสีทที่คัดแยกได้มีลักษณะทางสัณฐานวิทยาแตกต่างกัน ซึ่งสามารถแบ่งออกได้เป็น 9 สกุล คือ สกุล Streptomyces 35 ไอโซเลต สกุล Micromonospora 3 ไอโซเลต สกุล Microbispora 9 ไอโซเลต สกุล Microtetraspora 6 ไอโซเลต สกุล Actinomadura 2 ไอโซเลต สกุล Nocardia 4 ไอโซเลต สกุล Actinoallomurus 1 ไอโซเลต สกุล Dactylosporangium 3 ไอโซเลต Nonomuraea 1 ไอโซเลต และเป็นแอคติโนมัยสีทที่ไม่สามารถจำแนกได้อีกจำนวน 13 ไอโซเลต จากการศึกษาลำดับเบสในช่วง16S rRNA gene ของตัวแทนไอโซเลตในแต่ละสกุล พ
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วิเวกแว่ว, อัมพร, та วิเชฏฐ์ คนซื่อ. ความหลากหลายทางพันธุกรรมของอึ่งบางชนิดในสกุล Microhyla ในพื้นที่สวนสัตว์เปิดเขาเขียว จังหวัดชลบุรี โดยวิเคราะห์จากลำดับนิวคลีโอไทด์ ของยีน COI และยีน 16S rRNA ในไมโทคอนเดรียลดีเอ็นเอ. จุฬาลงกรณ์มหาวิทยาลัย, 2014. https://doi.org/10.58837/chula.res.2014.55.

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การผสมข้ามสายพันธุ์ (hybridization) เป็นการผสมพันธุ์ระหว่างสิ่งมีชีวิตที่มีความใกล้ชิดกันเชิงวิวัฒนาการที่มีโครงสร้างทางพันธุกรรมของประชากรหรือสปีชีส์ที่แตกต่างกัน ทั้งนี้ลูกผสม (hybrids) ที่เกิดขึ้นอาจทำให้เกิดการถ่ายเทเคลื่อนย้ายของยีน (gene flow) ระหว่างประชากรหรือสปีชีส์ได้หากลูกผสมดังกล่าวสามารถอยู่รอดและสืบพันธุ์ได้ ซึ่งสามารถตรวจสอบได้โดยใช้เทคนิคทางด้านอณูชีววิทยา อึ่งน้ำเต้า (Microhyla fissipes) อึ่งข้างดำ (M. heymonsi) และอึ่งลายเลอะ (M. butleri) จัดเป็นสัตว์สะเทินน้ำสะเทินบกที่อยู่ในสกุลเดียวกัน มีขนาดลำตัวใกล้เคียงกันและมีการกระจายอยู่ในทุกภาคของประเทศไทย จากการสำรวจเบื้องต้นพบว่าอ
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Chirthaworn, Chintana, Kanitha Patarakul, Duangporn Phulsuksombati, and Yong Pooworawan. Study of pathology and pathogenesis of leptospirosis in an animal model inoculated with various in vivo passages of leptospires : implications for vaccine development and diagnosis. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.26.

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Leptospirosis is a worldwide zoonosis caused by bacteria from the genus leptospira. Several reports have suggested the roles of cytokines in pathogenesis of this infectious disease. In this study, cytokine gene expression in kidneys livers and lung was investigated. In addition to host cytokine gene expression, the expression of leptospira genes at target organs was also studied. Golden Syrian hamsters were injected with virulent or avirulent leptospira interrogans serovar pyrogenes. Kidney liver and lung tissues were collected for pathological examination and gene expression. RNA was extracte
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Thurston, Alison, Karen Foley, Shelby Rosten, Susan Taylor, Robert Haehnel, and Robyn Barbato. Microbial activity in dust-contaminated Antarctic snow. Engineer Research and Development Center (U.S.), 2023. http://dx.doi.org/10.21079/11681/47681.

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During weather events, particles can accumulate on the snow near the Pegasus ice and Phoenix compacted-snow Runways at the US McMurdo Station in Antarctica. The deposited particles melt into the surface, initially forming steep-sided holes, which can widen into patches of weak and rotten snow and ice. These changes negatively impact the ice and snow runways and snow roads trafficked by vehicles. To understand the importance of microbes on this process, we examined deposited dust particles and their microbial communities in snow samples collected near the runways. Snow samples were analyzed at
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Davis, Robert E., Edna Tanne, James P. Prince, and Meir Klein. Yellow Disease of Grapevines: Impact, Pathogen Molecular Detection and Identification, Epidemiology, and Potential for Control. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568792.bard.

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Grapevine yellows diseases characterized by similar symptoms have been reported in several countries including Israel, the United States, France, Italy, Spain, Germany and Australia. These diseases are among the most serious known in grapevine, but precise knowledge of the pathogens' identities and modes of their spread is needed to devise effective control stratgegies. The overall goals of this project were to develop improved molecular diagnostic procedures for detection and identification of the presumed mycoplasmalike organism (MLO) pathogens, now termed phytoplasmas, and to apply these pr
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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of
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Zchori-Fein, Einat, Judith K. Brown, and Nurit Katzir. Biocomplexity and Selective modulation of whitefly symbiotic composition. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7591733.bard.

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Whiteflies are sap-sucking insects that harbor obligatory symbiotic bacteria to fulfill their dietary needs, as well as a facultative microbial community with diverse bacterial species. The sweetpotato whitefly Bemisia tabaci (Gennadius) is a severe agricultural pest in many parts of the world. This speciesconsists of several biotypes that have been distinguished largely on the basis of biochemical or molecular diagnostics, but whose biological significance is still unclear. The original objectives of the project were (i) to identify the specific complement of prokaryotic endosymbionts associa
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