Academic literature on the topic 'Protein display'

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Journal articles on the topic "Protein display"

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Wen, Fei, Dhruv K. Sethi, Kai W. Wucherpfennig, and Huimin Zhao. "Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display." Protein Engineering, Design and Selection 24, no. 9 (2011): 701–9. http://dx.doi.org/10.1093/protein/gzr035.

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Rice, Jeffrey J., and Patrick S. Daugherty. "Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides." Protein Engineering, Design and Selection 21, no. 7 (2008): 435–42. http://dx.doi.org/10.1093/protein/gzn020.

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He, M. "Ribosome display: Cell-free protein display technology." Briefings in Functional Genomics and Proteomics 1, no. 2 (2002): 204–12. http://dx.doi.org/10.1093/bfgp/1.2.204.

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Kościelska, K., L. Kiczak, M. Kasztura, O. Wesołowska, and J. Otlewski. "Phage display of proteins." Acta Biochimica Polonica 45, no. 3 (1998): 705–20. http://dx.doi.org/10.18388/abp.1998_4210.

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In recent years the phage display approach has become an increasingly popular method in protein research. This method enables the presentation of large peptide and protein libraries on the surface of phage particles from which molecules of desired functional property(ies) can be rapidly selected. The great advantage of this method is a direct linkage between an observed phenotype and encapsulated genotype, which allows fast determination of selected sequences. The phage display approach is a powerful tool in generating highly potent biomolecules, including: search for specific antibodies, dete
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Schimmele, Bernhard, Nico Gräfe, and Andreas Plückthun. "Ribosome display of mammalian receptor domains." Protein Engineering, Design and Selection 18, no. 6 (2005): 285–94. http://dx.doi.org/10.1093/protein/gzi030.

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Van Deventer, James A., Ryan L. Kelly, Saravanan Rajan, K. Dane Wittrup, and Sachdev S. Sidhu. "A switchable yeast display/secretion system." Protein Engineering Design and Selection 28, no. 10 (2015): 317–25. http://dx.doi.org/10.1093/protein/gzv043.

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Petrenko, V. A., G. P. Smith, M. M. Mazooji та T. Quinn. "α-Helically constrained phage display library". Protein Engineering, Design and Selection 15, № 11 (2002): 943–50. http://dx.doi.org/10.1093/protein/15.11.943.

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Daugherty, P. S., G. Chen, M. J. Olsen, B. L. Iverson, and G. Georgiou. "Antibody affinity maturation using bacterial surface display." Protein Engineering Design and Selection 11, no. 9 (1998): 825–32. http://dx.doi.org/10.1093/protein/11.9.825.

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Grage, Katrin, Verena Peters, and Bernd H. A. Rehm. "Recombinant Protein Production byIn VivoPolymer Inclusion Display." Applied and Environmental Microbiology 77, no. 18 (2011): 6706–9. http://dx.doi.org/10.1128/aem.05953-11.

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ABSTRACTA novel approach to produce purified recombinant proteins was established. The target protein is produced as polyhydroxyalkanoate (PHA) synthase fusion protein, which mediates intracellular formation of PHA inclusions displaying the target protein. After isolation of the PHA inclusions, the pure target protein was released by simple enterokinase digestion.
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Zahn, G., A. Skerra, and W. Höhne. "Investigation of a tetracycline-regulated phage display system." Protein Engineering, Design and Selection 12, no. 12 (1999): 1031–34. http://dx.doi.org/10.1093/protein/12.12.1031.

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Dissertations / Theses on the topic "Protein display"

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Nilsson, Mikael. "Protein-DNA recognition : in vitro evolution and characterization of DNA-binding proteins /." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4269.

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Löfblom, John. "Staphylococcal surface display for protein engineering and characterization." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

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Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. Directed evolution has provided researchers with a tool for circumventing our limited knowledge and hence the possibility to create novel molecules that by no means could have been designed today. The approach is based on construction of high-complexity combinatorial librarie
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Löfblom, John. "Staphylococcal surface display for protein engineering and characterization /." Stockholm : Bioteknologi, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

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Wheeler, Lucas. "The Evolution of Metal and Peptide Binding in the S100 Protein Family." Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23178.

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Proteins perform an incredible array of functions facilitated by a diverse set of biochemical properties. Changing these properties is an essential molecular mechanism of evolutionary change, with major questions in protein evolution surrounding this topic. How do new functional biochemical features evolve? How do proteins change following gene duplication events? I used the S100 protein family as a model to probe these aspects of protein evolution. The S100s are signaling proteins that play a diverse range of biological roles binding Calcium ions, transition metal ions, and other prote
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Mizrachi, Eshchar. "Solubility, particle formation and immune display of trimers of major capsid protein 7 of African horsesickness virus fused with enhanced green fluorescent protein." Diss., Pretoria : [s.n.], 2009. http://upetd.up.ac.za/thesis/available/etd-06082009-161825.

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Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/30392/1/Andrew_Pow_Thesis.pdf.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precurso
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Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Queensland University of Technology, 2008. http://eprints.qut.edu.au/30392/.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precurso
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Gragg, Megan Ellen. "Mutant Rhodopsins in Autosomal Dominant Retinitis Pigmentosa Display Variable Aggregation Properties." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1522935340252319.

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Kronqvist, Nina. "Staphylococcal surface display in directed evolution." Doctoral thesis, KTH, Molekylär Bioteknologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11555.

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Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. In this approach, generally termed directedevolution, high-complexity combinatorial libraries are created from which affinity proteins areisolated using an appropriate selection method, thus cir
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Meyer, Scott C. "Non-Covalent Selection Methodologies Utilizing Phage Display." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/194059.

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In nature, non-covalent interactions are as important and dynamic as they are elusive. As such, the study of non-covalent interactions both in vivo and in vitro has proven to be challenging. Given the potential benefits of elucidating protein-protein, ligand-receptor, and other biologically relevant interactions, the development of methodologies for the study of non-covalent interactions is an attractive goal.Biologically encoded protein and peptide libraries that connect the genotypic information with the expressed phenotype have emerged in recent years as powerful methods for studying non
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Books on the topic "Protein display"

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Carnazza, Santina. Phage display as a tool for synthetic biology. Nova Science Publishers, 2010.

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1950-, Guglielmino Salvatore, ed. Phage display as a tool for synthetic biology. Nova Science Publishers, 2010.

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Rangwala, Huzefa. Introduction to protein structure prediction: Methods and algorithms. Wiley, 2010.

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K, Kay Brian, Winter Jill, and McCafferty John Dr, eds. Phage display of peptides and proteins: A laboratory manual. Academic Press, 1996.

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Lambert-Giroux, Suzanne. Skeletal muscles lacking Bpag1 crosslinking proteins display aberrant cytoskeletal features. School of Graduate Studies, Laurentian University, 2005.

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S, Sidhu Sachdev, ed. Phage display in biotechnology and drug discovery. Taylor & Francis/CRC Press, 2005.

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Senate, United States Congress. A bill to require the Commissioner of Social Security to issue uniform standards for the method of truncation of Social Security account numbers in order to protect such numbers from being used in the perpetration of fraud or identity theft and to provide for a prohibition on the display to the general public on the Internet of Social Security account numbers by State and local government, and for other purposes. U.S. G.P.O., 2008.

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Douthwaite, Julie A., and Ronald H. Jackson. Ribosome Display and Related Technologies: Methods and Protocols. Humana Press, 2016.

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Ribosome display and related technologies: Methods and protocols. Humana Press, 2012.

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Karypis, George, and Huzefa Rangwala. Introduction to Protein Structure Prediction. Wiley & Sons, Incorporated, John, 2010.

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Book chapters on the topic "Protein display"

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Urvoas, Agathe, Philippe Minard, and Patrice Soumillion. "Protein Engineering by Phage Display." In Protein Engineering Handbook. Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634026.ch24.

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Hackel, Benjamin J., and Dane Wittrup. "Yeast Surface Display in Protein Engineering and Analysis." In Protein Engineering Handbook. Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634026.ch26.

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Buchanan, Andrew. "Evolution of Protein Stability Using Ribosome Display." In Ribosome Display and Related Technologies. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-379-0_11.

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Kaltenbach, Miriam, and Florian Hollfelder. "SNAP Display: In Vitro Protein Evolution in Microdroplets." In Ribosome Display and Related Technologies. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-379-0_7.

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Hertveldt, Kirsten, Tim Beliën, and Guido Volckaert. "General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein–Protein Interactions." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-565-1_19.

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Medina-Cucurella, Angelica V., and Timothy A. Whitehead. "Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display." In Protein Complex Assembly. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7759-8_7.

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Ton-That, Hung, Sarkis K. Mazmanian, Gwen Liu, and Olaf Schneewind. "Surface Protein Anchoring and Display in Staphylococci." In Infectious Agents and Pathogenesis. Springer US, 2001. http://dx.doi.org/10.1007/0-306-46848-4_9.

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Kastelic, Damjana, and Mingyue He. "Ribosome Display and Screening for Protein Therapeutics." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-921-1_4.

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Cotten, Steve W., Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu. "mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries." In Ribosome Display and Related Technologies. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-379-0_16.

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Unkauf, Tobias, Sebastian Miethe, Viola Fühner, Thomas Schirrmann, André Frenzel, and Michael Hust. "Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy." In Protein Targeting Compounds. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-22473-2_4.

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Conference papers on the topic "Protein display"

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Chiang, Chang-Yue, Chien-Hsing Chen, Chien-Tsung Wang, Chin-Wei Wu, and Hsing-Yu Chiang. "Trace Determination of Cardiac Troponin I Using a Label-Free Fiber Optical Localized Surface Plasmon Resonance Biosensors." In 3D Image Acquisition and Display: Technology, Perception and Applications. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/3d.2024.jm4a.10.

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This work proposes a carboxyl-graphene-oxide-based fiber optic localized surface plasmon resonance biosensor which employed anti-Cardiac Troponin I (anti-cTnI) as the recognition element to detect the cTnI-protein in 10 min with a limit of detection (LOD) of 5.8 pM, which meets the acceptable LOD for clinical testing of 0.04 ng/mL.
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Kandori, Ayumu, Masato Takahashi, Chawan Koopipat, and Norimichi Tsumura. "Proteus effect in vertical jumping wearing a head-mounted display." In 3D Image Acquisition and Display: Technology, Perception and Applications. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/3d.2024.jth2a.10.

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Wright, Nathan, and Chris Burneka. "Dynamic Performance Assessment of Side Facing Troop Seats During Impact." In Vertical Flight Society 73rd Annual Forum & Technology Display. The Vertical Flight Society, 2017. http://dx.doi.org/10.4050/f-0073-2017-12039.

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The Air Force Research Laboratory Aircrew Biodynamics and Protection Group of the Applied Neuroscience Branch (711HPW/RHCPT) conducted a dynamic impact comparative test program of currently-fielded side facing troop seats to evaluate how effectively the seats protect occupants ranging from the 5th percentile female to the 98th percentile male during crash events. The test program consisted of impact testing stock H-60A/L, CV-22, and CH-53E seats and quantifying the safety effectiveness of each seat using recommended injury criteria from the Full Spectrum Crashworthiness (FSC) report and other
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Kumar, R., D. Thenarasu, R. Ravindranath, and D. B. Chalwade. "Damage Behaviour of Rotorcraft End Plate under High Velocity Bird Impact." In Vertical Flight Society 72nd Annual Forum & Technology Display. The Vertical Flight Society, 2016. http://dx.doi.org/10.4050/f-0072-2016-11525.

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The finite element model is developed for simulating the bird strike test of rotorcraft End plate using the bird numerical modeling techniques. The main design goal is the satisfactory impact resistance of the composite structures in the case of bird strike. The design concept is based on the absorption of the major portion of the bird kinetic energy by the composite skins, in order to protect the inner honeycomb core from damage, thus preserving the End plate functionality for safe landing. To this purpose, the End plate skin is fabricated from composite layers, which unfold under the impact
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Larose, Guy, Maryam Al Labbad, Sharon Schajnoha, and Junting Chen. "A Comparative Study of the Use of Experimental and Computational Techniques to Assess Turbulence Above the FATO to Support the Design and Operations of Vertiports in the Built Environment." In Vertical Flight Society 81st Annual Forum and Technology Display. The Vertical Flight Society, 2025. https://doi.org/10.4050/f-0081-2025-186.

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Turbulence conditions at hospital heliports in the built environment are routinely assessed at the design stage through experimental, physical testing in boundary layer wind tunnels. Wind tunnel testing is the gold standard to evaluate wind conditions on and around buildings where human safety is of the upmost concern. Numerical techniques, such as computation fluid dynamics (CFD) are continuously improving and may offer a viable alternative to wind tunnel testing in some cases. Within the CFD toolbox, there are several techniques to simulate a flow field in an urban or suburban context. These
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Gilliland, Gary. "Security in Avionics Systems." In Vertical Flight Society 80th Annual Forum & Technology Display. The Vertical Flight Society, 2024. http://dx.doi.org/10.4050/f-0080-2024-1061.

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There is a shift in the industry driving avionics manufactures to provide more interactive connectivity than they have had to in the past. The increasing threat of cyber security attacks in our communication systems is an increasing problem in our society and the avionics industry cannot ignore the fact that the threats are real and they must protect the systems from these attacks. Another element driving these concerns is the implementation of the FAA's NextGen or EASA's SESAR technologies which will require avionics vendors to replace their proprietary, relatively isolated embedded computer
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Pellettiere, Joseph, and Amanda Taylor. "Anthropomorphic Test Device Response in Vertical Drop Tests and Sled Tests." In Vertical Flight Society 74th Annual Forum & Technology Display. The Vertical Flight Society, 2018. http://dx.doi.org/10.4050/f-0074-2018-12735.

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The Federal Aviation Administration has regulations designed to protect aircraft occupants in the event of a crash. Two-drop tests of a Fokker 28 (F-28) small regional jet airframe performed at the National Aeronautics and Space Agency provided full-scale crash data on the performance of transport category aircraft seating systems. The crash pulse measured at the floor differed from the certification tests and the performance of the seats did as well. To assess injuries in the spinal column, lumbar loads were collected for all Anthropomorphic Test Devices, and a range of occupants was included
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Palacios, Jose, Joseph Szefi, Douglas Wolfe, and Matthew Bailey. "Ice Testing of a Centrifugally Powered Pneumatic Deicing System for Helicopter Rotor Blades." In Vertical Flight Society 70th Annual Forum & Technology Display. The Vertical Flight Society, 2014. http://dx.doi.org/10.4050/f-0070-2014-9662.

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A novel pneumatic approach to protect helicopter rotor blades from ice accretion is presented in this paper. The system relies on centrifugally generated pressures to deform a 0.508 mm (0.02 in.) thick titanium leading edge cap. The leading edge cap is protected by a 10 μm (390 microinch) thick Ti-Al-N erosion resistant coating. Beneath the titanium leading edge, six (6) pneumatic diaphragms were installed. The diaphragms are normally deflated under vacuum against the surface of the blade, and are inflated when ice accretion thickness reaches a critical value. The deformation of the leading ed
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Pellettiere, Joseph, and Amanda Taylor. "Transport Aircraft Seating in Rotorcraft Crash Testing." In Vertical Flight Society 72nd Annual Forum & Technology Display. The Vertical Flight Society, 2016. http://dx.doi.org/10.4050/f-0072-2016-11412.

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The Federal Aviation Administration (FAA) has standards and regulations that are designed to protect aircraft occupants in the event of a crash. These standards focus primarily on horizontal and vertical impact protection, and measure the dynamic performance of the seating system and occupant restraints. Currently no requirement for full-scale crashworthiness testing exists due to the cost prohibitive nature of conducting such a test. The requirements were developed through review of research, existing requirements, modeling and simulation, and accident analysis. A drop of a Transport Rotorcra
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Pellettiere, Joseph, Robert Huculak, and Richard DeWeese. "Evaluation of Aerospace Seat Belt Webbing Material Under Dynamic Test Conditions." In Vertical Flight Society 72nd Annual Forum & Technology Display. The Vertical Flight Society, 2016. http://dx.doi.org/10.4050/f-0072-2016-11413.

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There is no requirement for full-scale testing of either civil or military rotorcraft to certify a design as safe or crashworthy. The Federal Aviation Administration has a number of standards and regulations that are designed to protect occupants in the event of a crash. These standards focus primarily on frontal and vertical impact protection of the occupant seating system and those items in the cabin interior that surround the occupant. With the adoption of Title 14 Code of Federal Regulations (CFR) 29.562, as well as the corresponding portions of 14 CFR 23.562, 25.562, and 27.562, a seating
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Reports on the topic "Protein display"

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Epel, Bernard L., Roger N. Beachy, A. Katz, et al. Isolation and Characterization of Plasmodesmata Components by Association with Tobacco Mosaic Virus Movement Proteins Fused with the Green Fluorescent Protein from Aequorea victoria. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7573996.bard.

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The coordination and regulation of growth and development in multicellular organisms is dependent, in part, on the controlled short and long-distance transport of signaling molecule: In plants, symplastic communication is provided by trans-wall co-axial membranous tunnels termed plasmodesmata (Pd). Plant viruses spread cell-to-cell by altering Pd. This movement scenario necessitates a targeting mechanism that delivers the virus to a Pd and a transport mechanism to move the virion or viral nucleic acid through the Pd channel. The identity of host proteins with which MP interacts, the mechanism
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Morrison, Mark, and Joshuah Miron. Molecular-Based Analysis of Cellulose Binding Proteins Involved with Adherence to Cellulose by Ruminococcus albus. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7695844.bard.

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At the beginning of this project, it was clear that R. albus adhered tightly to cellulose and its efficient degradation of this polysaccharide was dependent on micromolar concentrations of phenylacetic acid (PAA) and phenylpropionic acid (PPA). The objectives for our research were: i) to identify how many different kinds of cellulose binding proteins are produced by Ruminococcus albus; ii) to isolate and clone the genes encoding some of these proteins from the same bacterium; iii) to determine where these various proteins were located and; iv) quantify the relative importance of these proteins
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Ohad, Nir, and Robert Fischer. Control of Fertilization-Independent Development by the FIE1 Gene. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575290.bard.

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A fundamental problem in biology is to understand how fertilization initiates reproductive development. During plant reproduction, one sperm cell fuses with the egg to form an embryo, whereas a second sperm cell fuses with the adjacent central cell nucleus to form the endosperm tissue that supports embryo and/or seedling development. To understand the mechanisms that initiate reproduction, we have isolated mutants of Arabidopsis that allow for replication of the central cell and subsequent endosperm development without fertilization. In this project we have cloned the MEA gene and showed that
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Tucker, Mark L., Shimon Meir, Amnon Lers, Sonia Philosoph-Hadas, and Cai-Zhong Jiang. Elucidation of signaling pathways that regulate ethylene-induced leaf and flower abscission of agriculturally important plants. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597929.bard.

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The Problem: Abscission is a highly regulated process, occurring as a natural terminal stage of development, in which various organs are separated from the parent plant. In most plant species, the process is initiated by a decrease in active auxin in the abscission zone (AZ) and an increase in ethylene, and may be accelerated by postharvest or environmental stresses. Another potential key regulator in abscission is IDA (Inflorescence Deficient in Abscission), which was identified as an essential peptide signal for floral organ abscission in Arabidopsis. However, information is still lacking re
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Eyal, Yoram, and Sheila McCormick. Molecular Mechanisms of Pollen-Pistil Interactions in Interspecific Crossing Barriers in the Tomato Family. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573076.bard.

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During the evolutionary process of speciation in plants, naturally occurring barriers to reproduction have developed that affect the transfer of genes within and between related species. These barriers can occur at several different levels beginning with pollination-barriers and ending with hybrid-breakdown. The interaction between pollen and pistils presents one of the major barriers to intra- and inter-specific crosses and is the focus of this research project. Our long-term goal in this research proposal was defined to resolve questions on recognition and communication during pollen-pistil
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Dickman, Martin B., and Oded Yarden. Genetic and chemical intervention in ROS signaling pathways affecting development and pathogenicity of Sclerotinia sclerotiorum. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7699866.bard.

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Abstract: The long-term goals of our research are to understand the regulation of sclerotial development and pathogenicity in S. sclerotior11111. The focus in this project was on the elucidation of the signaling events and environmental cues involved in the regulation of these processes, utilizing and continuously developing tools our research groups have established and/or adapted for analysis of S. sclerotiorum, Our stated objectives: To take advantage of the recent conceptual (ROS/PPs signaling) and technical (amenability of S. sclerotiorumto manipulations coupled with chemical genomics and
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Pianwanit, Somsak, and Sirirat Kokpol. Theoretical analysis of photoinduced electron transfer in FMN binding protein : Effect of changes in one charge on electron transfer rate. Chulalongkorn University, 2013. https://doi.org/10.58837/chula.res.2013.32.

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Photoinduced electron transfer (PET) is an important process due to its several applications, e.g. solar energy conversion. Flavoproteins are generally selected as a model for the study of PET. In this research, effect of charge at residue 13 on the PET from Trp32, Tyr35 and Try106 to an excited isoalloxazine (Iso*) in FMN binding protein (FBP) from Desulfovibrio vulgaris (Miyazaki F) was studied. A wild type (E13 with negative charge) and four mutations of FBP at residue 13, E13K and E13R (politive charge), E13T and E13Q (neutral charge), were subjected to molecular dynamics (MD) simulations,
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Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

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Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TI
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Chejanovsky, Nor, and Suzanne M. Thiem. Isolation of Baculoviruses with Expanded Spectrum of Action against Lepidopteran Pests. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7586457.bard.

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Our long-term goal is to learn to control (expand and restrict) the host range of baculoviruses. In this project our aim was to expand the host range of the prototype baculovirus Autographa cali/arnica nuclear polyhedrosis virus (AcMNPV) towards American and Israeli pests. To achieve this objective we studied AcMNPV infection in the non-permissive hosts L. dispar and s. littoralis (Ld652Y and SL2 cells, respectively) as a model system and the major barriers to viral replication. We isolated recombinant baculoviruses with expanded infectivity towards L. dispar and S. littoralis and tested their
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Prusky, Dov, Noel Keen, and John Browse. Modulation of the synthesis of the main preformed antifungal compound as abasis for the prevention of postharvest disease of C. gloeosporioides in avocado fruits. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7575273.bard.

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The most important pathological factor limiting fruit life after harvest in subtropical fruits are quiescent infections of anthracnose caused by Colletotrichum gloeosporioides. Prusky and Keen elucidated the mechanism of resistance in avocado fruits to quiescent infections of C. gloeosporioides and determined that the major biocide involved is the preformed compound,1-acetoxy-2-hydroxy-4-oxo-heneicosa-13, 15 diene. Two possibilities exist for maintaining fungitoxic levels of antifungal compounds in the tissue of ripening fruits: (i). Prevention of catabolism (ii). Induction of synthesis. Previ
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