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Dissertations / Theses on the topic 'Protein Preparation'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Guzman-Juarez, M. "Yeast protein products : Preparation, characterisation and functional properties." Thesis, University of Reading, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376661.

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2

Christie, Patricia Dianne 1967. "Preparation of isotopically labeled MoFe protein of nitrogenase." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/11229.

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3

Alzahrani, Eman Saad. "Investigation of monolithic materials for protein sample preparation." Thesis, University of Hull, 2012. http://hydra.hull.ac.uk/resources/hull:7149.

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Proteomics plays an important role in the recognition of diseases and the understanding of biological processes. Sample preparation is a bottleneck in systems for chemical analysis and it is a required step in proteomics in order to remove interferences and preconcentrate the proteins. In addition, protein reduction and alkylation before digestion is a required step in proteomics to facilitate protein unfolding and increase the efficiency of enzymes in digesting proteins. The purpose of this study was to develop new techniques to address some of the shortcomings of current sample preparation m

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4

Shiozaki, Aiko. "Proteome analysis of brain proteins in Alzheimer's disease : Subproteomics following sequentially extracted protein preparation." Kyoto University, 2004. http://hdl.handle.net/2433/147486.

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5

Moosavi-Nasab, Marzieh. "Protein structural changes during preparation and storage of surimi." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84297.

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Myofibrillar proteins, the main components that impart functional properties to muscle foods, can undergo denaturation and aggregation during frozen storage. The overall objective of this research was to study the changes in protein structure that are associated with the preparation and frozen storage of surimi. In addition, the relative cryoprotective effects of whey protein concentrate, whey protein isolate, soy protein isolate, flaxseed meal and flaxseed protein were assessed in surimi during storage.<br>Raw surimi was prepared by repeatedly washing Alaska pollock flesh with chilled

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6

Kittivoravitkul, Sasitorn. "Preparation, characterisation and in vivo evaluation of DHEA protein conjugates." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250667.

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7

GÓMEZ, PÉREZ INMACULADA JÉNNIFER. "DESIGN AND PREPARATION OF CARBON NANODOTS FOR BIOMEDICAL APPLICATIONS." Doctoral thesis, Università degli Studi di Trieste, 2018. http://hdl.handle.net/11368/2919821.

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In the last few decades great efforts have been placed in studying carbon nanomaterials. Nowadays the nanocarbon family spans from fullerene, the first member, to Carbon Dots (C-Dots), the last to join. C-Dots have attracted considerable attention for a wide range of applications, especially bioapplications, because of their properties: emission, small sizes, aqueous solubility, biocompatibility and chemical stability. The aim of this doctoral thesis was to synthesize Carbon nanodots (CNDs), in particular nitrogen-doped CNDs, and functionalize their surface with properly designed molecules/bio

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8

Cervantes-Laurean, Daniel. "Preparation and Characterization of Model Conjugates for the Study of Proteins Modified by ADP-ribose." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc935701/.

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Modification of proteins by ADP-ribose has been shown to be a versatile modification with respect to the amino acid side chain. The results described here will allow the study of the biological importance of ADP-ribose glycation and also allow differentiation on crude extracts between enzymatic modifications from protein ADP-ribose glycation that can occur due to the presence of NAD glycohydrolases.

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9

Jansson, Eva. "Blood protein coated model biomaterials : preparation, and cell and tissue response /." Linköping : Univ, 2003. http://www.bibl.liu.se/liupubl/disp/disp2003/tek798s.pdf.

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10

Calvert, Elaine. "Preparation and characterisation of a multichain protein from the wool microfibril." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235590.

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11

Nge, Pamela Nsang. "Microfluidic Devices with Integrated Sample Preparation for Improved Analysis of Protein Biomarkers." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3918.

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Biomarkers present a non-invasive means of detecting cancer because they can be obtained from body fluids. They can also be used for prognosis and assessing response to treatment. To limit interferences it is essential to pretreat biological samples before analysis. Sample preparation methods include extraction of analyte from an unsuitable matrix, purification, concentration or dilution and labeling. The many advantages offered by microfluidics include portability, speed, automation and integration. Because of the difficulties encountered in integrating this step in microfluidic devices most

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12

Lambert, Elizabeth M. "Organization of protein biomolecules for the template-assisted preparation of higher-order inorganic materials." Thesis, University of Bristol, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.535170.

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13

Steen, Johanna. "Characterization of Antigenic Properties and High Throughput Protein Purification." Doctoral thesis, KTH, Proteomik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-25841.

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To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. Another obstacle for affinity proteomics is the generation of high quality antibodies, invaluable tools in biotechnological applications. The objective in this thesis was to facilitate protein purification and sample preparation before analysis and downstream applications. We also aimed

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14

Hiller, Matthias. "Sample preparation of membrane proteins suitable for solid-state MAS NMR and development of assignment strategies." Phd thesis, Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2009/3724/.

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Although the basic structure of biological membranes is provided by the lipid bilayer, most of the specific functions are carried out by membrane proteins (MPs) such as channels, ion-pumps and receptors. Additionally, it is known, that mutations in MPs are directly or indirectly involved in many diseases. Thus, structure determination of MPs is of major interest not only in structural biology but also in pharmacology, especially for drug development. Advances in structural biology of membrane proteins (MPs) have been strongly supported by the success of three leading techniques: X-ray crystall

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15

Pierini, Giulia. "Preparation and characterization of grafted nonwoven membranes for bioseparations." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/11903/.

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Protein purification plays a crucial role in biotechnology and biomanufacturing, where downstream unit operations account for 40%-80% of the overall costs. To overcome this issue, companies strive to simplify the separation process by reducing the number of steps and replacing expensive separation devices. In this context, commercially available polybutylene terephthalate (PBT) melt-blown nonwoven membranes have been developed as a novel disposable membrane chromatography support. The PBT nonwoven membrane is able to capture products and reduce contaminants by ion exchange chromatography. The

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16

Yon, Suzanne Michele. "The preparation and application of monoclonal antibodies specific for phosphorylated isoforms of myelin basic protein." Thesis, Oxford Brookes University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261505.

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17

Parekh, Khushboo K. "Preparation, Characterization, and In Vitro Protein Release Studies in Pharmaceutically relevant Lecithin Microemulsions." University of Toledo Health Science Campus / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=mco1300753350.

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18

Feng, Wei Zhu Shiping. "Surface modification through atom transfer radical polymerization grafting for the preparation of protein-resistant materials." *McMaster only, 2007.

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19

Geier, George Richard. "The preparation of a metalloporphyrin-peptide conjugate artificial protein for the catalytic oxidation of alkenes /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/8546.

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20

Shrestha, Prashanta. "Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3917.

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This thesis reports the viability of E. coli cell extracts prepared using equipment that is both common to biotechnology laboratories and able to process small volume samples and expression of proteins containing unnatural amino acids (UAAs) at higher level using PCR amplified linear DNA templates (LETs) in cell-free protein synthesis (CFPS) system. E. coli-based cell extracts are a vital component of inexpensive and high-yielding CFPS reactions. However, effective preparation of E. coli cell extract is limited to high-pressure homogenizers (French press style or impinge-style) or bead mill ho

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21

Al-Saadi, Ali. "Preparation and characterisation of encapsulation magnetic metal iron oxide nanoparticles." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:57bdcf38-9d45-48ab-a971-a2d60e2e4391.

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One of the most challenging goals in nanoparticle research is to develop successful protocols for the large-scale, simple and possibly low-cost preparation of morphologically pure nanoparticles with enhanced properties. The work presented in this thesis was focused on the synthesis, characterisation and testing of magnetic nanoparticles and their potential applications. There are a number of magnetic nano-materials prepared for specific applications such as metal oxide nanoparticles encapsulated with various porous materials including Fe₃O₄/Fe₂O₃ coated with soft bio-organic materials such as

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22

Murphy, Anastasia V. "Preparation of structurally diverse C-linked antifreeze glycoprotein analogs and assessment for antifreeze protein-specific activity." Diss., Online access via UMI:, 2005. http://wwwlib.umi.com/dissertations/fullcit/3159276.

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23

Ferguson, Stephen Scott. "Design, Preparation, and Characterization of Metallo-Adducts of the Histidine-Containing Phosphocarrier Protein from Bacillis Subtilis." NCSU, 2000. http://www.lib.ncsu.edu/theses/available/etd-20000120-044505.

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<p>Ferguson, Stephen Scott. The expression, purification, and characterization of metallo-adducts of the histidine-containing phosphocarrier protein (HPr) from Bacillus Subtilis and two of its engineered mutants. (Under the direction of Charles R. Cornman)Expression of recombinant HPr and its mutants HPrgamma?n (S12Y, R17H, E84Y) and HPrbeta (S12Y, R17H) was performed in the Escherichia coli organism. The proteins were purified via calmodulin affinity resin to yields of ~40 mg/L of culture. The native HPr protein was shown to be active by mutant complementation assays, while the engineered mut

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24

Birchfield, Aaron. "Preparation of a Flavonol Specific Glucosyltransferase found in Grapefruit and Site-Directed Mutants for Protein Crystallization." Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/etd/3523.

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This research was designed to determine the conditions necessary to remove c-myc and 6x-His tags from a flavonol specific glucosyltransferase found in grapefruit (CP3GT) using thrombin in preparation for crystallization. X-ray crystallography of CP3GT crystals may elucidate structural features that account for flavonol specificity in some glucosyltransferase enzymes. A thrombin cleavage site was inserted into WT CP3GT and one mutant. Recombinant CP3GT was expressed in yeast and purified. Optimal conditions for thrombin digestion were explored. Digestion with 100U of thrombin for 2 hours at 4o

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25

ten, Brink Tim [Verfasser]. "Automated Structure Preparation and Its Influences on Protein-Ligand Docking and Virtual Screening / Tim ten Brink." Konstanz : Bibliothek der Universität Konstanz, 2011. http://d-nb.info/101745504X/34.

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26

Abouzeid, Ragab. "Advanced cellulose composites, preparation and properties." Phd thesis, Université de Grenoble, 2012. http://tel.archives-ouvertes.fr/tel-00947624.

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L'accumulation de déchets agricoles tels que la bagasse et le développement d'alternatives aux polymères issus de la pétrochimie ont reçu une attention croissante au cours des deux dernières décennies, due à l'augmentation de la population et à la préoccupation croissante pour la préservation de l'environnement. Cette étude tente de résoudre ces problèmes à l'aide de deux approches principales.La première est liée à des composites à base de papier et de liant naturel et la seconde est associée à la préparation et à la caractérisation de dérivés cellulosiques présentant des propriétés cristaux

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27

Knight, Derek Andrew. "Preparation of a site-specific lymphotoxin- mutant to be used in protein characterization and receptor binding studies." CSUSB ScholarWorks, 1995. https://scholarworks.lib.csusb.edu/etd-project/987.

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28

TIMMERMAN, JOHANNA. "Preparation and structural study of the dna-binding domain of the cyp1 protein of the yeast saccharomyces cerevisiae." Paris 11, 1994. http://www.theses.fr/1994PA112429.

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Dans la levure saccharomyces cerevisiae, la proteine cyp1 est un regulateur de la transcription de nombreux genes dont l'expression est dependante de l'oxygene. Nous nous sommes interesse a la determination de la structure tridimensionnelle du domaine de fixation a l'adn de cette proteine. Cette partie contient un motif cys-xaa(2)-cys-xaa(6)-cys-xaa(6)-cys-xaa(2)-cys-xaa(8)-cys, que l'on retrouve dans de nombreuses proteines de levure interagissant avec l'adn, en particulier dans la proteine gal4. Le but a long terme est de determiner le complexe cyp1-adn par resonance magnetique nucleaire. Da

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29

Naderi, Nassim. "Development of a fractionation process for the preparation of a folate-enriched protein extract from hen egg yolks." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26198.

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Le fractionnement du jaune d’œuf est une façon judicieuse d’étendre les domaines d’application de cet ingrédient dans les industries alimentaire et nutraceutique. Le but de ce projet a été de mettre au point un fractionnement non-toxique du jaune d’œuf, par centrifugation, dans l’objectif d’obtenir un produit enrichi en extrait naturel de folate. De par sa teneur élevée en cholestérol, le jaune d’œuf a été considéré comme un sous-produit du procédé de séparation des œufs. Cependant, le jaune d'œuf contient aussi une forme précieuse et biodisponible d'acide folique. La dilution du jaune d’œuf e

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30

Guidi, B. "ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES." Doctoral thesis, Università degli Studi di Milano, 2018. http://hdl.handle.net/2434/541057.

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This PhD project focuses on the identification, isolation and characterization of new biocatalysts able to generate biologically active molecules with significant enantioselectivity. Through screening, we identified marine strains, from MaCuMBA (Marine Culturable Microorganism for Biotechnological Applications) and BIODEEP (Biotechnologies form the deep) European project collections, which show a marked enantioselectivity on intermediates of molecules of biological interest. Biotransformation substrate range included pramipexole, as main target, but it also embraces other common building block

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31

Nguyen, Anh Mai. "New approaches to preparation of macroporous monoliths for use in liquid chromatography." Doctoral thesis, Umeå : Department of Chemistry, Umeå Univ, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-20890.

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32

Singh, A. K. "Studies on the diterpenes of Helianthus annuus : identification of a new gibberellin and preparation of protein conjugates of kaurenoids." Thesis, University of Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376496.

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33

Singh, Priti. "A New Interfacial Cross Linking Technique: Preparation, Characterization and Evaluation of Calcium alginate Nanoparticles as a Protein Delivery System." University of Toledo Health Science Campus / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=mco1365434442.

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34

Hsiao, Cheng-Yen, and 蕭正彥. "Substrates preparation for Microarray protein chips." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/w9u59h.

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碩士<br>國立臺北科技大學<br>有機高分子研究所<br>99<br>Protein microarray chips is composed of three parts, those are pre-treatment substrates, surface chemical modification, and immobilizing protein on substrate surfaces. In this study, self-assembly monolayers is used for surface chemical modification. By this method to perform silaneization on glass and silicon wafer, forming the terminal group substrates. Due to protein includes COOH and NH2 functional groups, that could bind with silane, dendrimer on substrates, so proteins will immobilize on substrate surface. Finally, we will connected to the substrates,

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35

Liu, Yu-Chih, and 劉昱志. "Preparation of the Akt Kinase Fusion Protein." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/62769377903313199696.

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碩士<br>國立中正大學<br>生命科學系暨分子生物研究所暨生物醫學研究所<br>98<br>Phostensin is a protein phosphatase 1 F-actin cytoskeleton targeting subunit. Phostensin is a F-actin-binding protein that binds to the pointed ends of actin filament and modulates actin dynamics. In our preliminary results, we found that Akt can phosphorylate phostensin. In order to study the effect on biochemical activity of phostensin by Akt phosphorylation, we have to prepare the active Akt kinase. Activation of Akt is occurred at the plasma membrane where Akt is phosphorylated at Thr-308 and Ser473. However, E. coli lacks the kinases that pho

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36

Chen, Chang-Jey, and 陳昌傑. "Protein expression and antibody preparation of dengue virus type 2 NS5 protein." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/60498711918857309912.

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37

Li, Cai-shan, and 李采珊. "Preparation and Surface Activity of Soy Protein Derivatives." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/498352.

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碩士<br>國立臺灣科技大學<br>高分子系<br>95<br>In this study, a series of polymeric surfactant is prepared by the reaction of soy protein, polyoxyethylenated stearyl ether and dextrin. The structures of these products were conformed by the measurement of FT-IR, 1H-NMR and EA analyses. The fundamental properties containing surface tension, foaming, wetting, emulsion, and fluorescence properties and application for scouring, bleaching and dyeing of surfactants have been evaluated. From the results, the good properties including surface tension, foaming and wetting properties were found. The surface tension inc

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38

Sun, Pai-Hsiu, and 孫百秀. "Preparation and Characterization of Isolated Soy Protein Hydrolysate – Nanoparticles." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/48572221476854677563.

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碩士<br>東海大學<br>食品科學系<br>99<br>Chitosan (Mw 475.5 kDa, deacetylation≧85%) and its hydrolysates (Mw 231.5 and 131.1 kDa, hydrolyzed by cellulase), were dissolved in 1% acetic acid solution and adjusted to pH 4.7, and added with isolated soy protein hydrolysate (ISPH) solutions (Mw 10~30 kDa molecular weights, 1~9 mg/mL concentrations) before mixing with sodium tripolyphosphate (TPP) solution (pH 9.0) to prepare CS-TPP-ISPH nanoparticles. Such effective factors as chitosan molecular weight, ISPH concentration and chitosan / TPP mass ratio on the encapsulation efficiency (EE), loading capacity (LC)

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39

Mercatelli, Eleonora. "Development of novel sample preparation strategies for in-cell NMR." Doctoral thesis, 2017. http://hdl.handle.net/2158/1114729.

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NMR spectroscopy is offering increasing possibilities to obtain structural and dynamic information about macromolecules at atomic resolution. In recent years, it has been extended to the investigation of biological macromolecules in their physiological environment. In-cell NMR spectroscopy allows obtaining physiologically relevant structural and functional information inside living cells through the direct observation of several processes such as protein folding and interaction, metal ion binding, and drugs screening. This thesis aims to widen the application of in-cell NMR for the characteriz

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40

Gordhan, Hasha. "Preparation of chemically modified transferrin proteins and an investigation of their reactions with DNA and other nucleic acids." Thesis, 1986. http://hdl.handle.net/10413/10098.

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The molecular biology of human genetic disorders is under intensive investigation at present. In those cases where the disorder is clearly defined in terms of altered gene structure, possibilities may exist for the correction of the disorder by insertion of normal genes through the process of DNA transfection. A possible method for the transfer of genetic material is by attempting to attach DNA to a protein which has specific receptors on cells and which undergoes receptor-mediated endocytosis. By this means one might be able to get DNA into cells. This thesis deals with experimental work on t

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41

Lin, Yin-Ju, and 林殷如. "Preparation of Antisera against Ripen-Related Protein Kinase in Banana." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/55899998566359948423.

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碩士<br>國立臺灣大學<br>園藝學研究所<br>89<br>According to the comparison between the amino acids encoded from banana cDNA pBPK6 ( Mh-PK1 ) and pBPK10 ( Mh- PK3 ), over 76 % of similarity exists in N-terminus, but low similarity was found in C-terminus. By using pQE expression vectors, the banana ripening-related protein kinases, including the C terminal part of Mh-PK1, the complete coding region of Mh-PK2b and C terminal part of Mh-PK3 were expressed in E. coli. The constructed plasmids are pQPK6, pQPK9, and pQPK10 for pBPK6, pBPK9, and pBPK10, respectively. The molecular mass of the fusion prot

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42

Chuang, Chien-Yi, and 莊建宜. "Preparation and Application of Non-Porous Adsorbents for Protein Chromatography." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/45045282089005600459.

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碩士<br>中原大學<br>化學工程研究所<br>81<br>Two kinds of monodispersed, non-porous chromatographic supports, i.e., non-porous PS particle with an average diameter of 2.2 μm and non-porous silica with an average diameter of 1.4μm, were prepared. The PS particle, due to its hydrophobic property, was first coated with polyethyleneimine (PEI) before an affinity ligand was permanently bounded onto its surface. The non-porous silica was silanized with r-aminopropyltriethoxy silane (APTES) to introduce termin

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43

Wong, Wei-Cheng, and 黃暐程. "Preparation and physicochemical properties of sweet potato leaf protein extractive." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/96858772553632248123.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>101<br>The demands for animal proteins in the food industry of world had increased greatly in conjunction with the fast growing economy. This leads to a serious shortage of protein sources. The sweet potato leaves are easily grown crops which contained large amount of proteins. The present study was to investigate the effectively utilize the sweet potato leaves protein (SPLP) in the investigation of the optimum extractive conditions, decolorized conditions, physicochemical properties, wastewater production and different extraction methods of SPLP. The protein extract

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44

Che-KaiChang and 張哲凱. "Multiplex sample preparation and search strategies for in-depth identification of serum proteins and protein modifications." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/51869259647821625967.

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碩士<br>國立成功大學<br>化學系<br>103<br>Human serum protein analysis by liquid chromatography-mass spectrometry (LC-MS) is a difficult task due to highly complicated biological matrix and a broad dynamic range covering thousands of serum proteins. In this study, we developed a multiplex sample preparation and search strategy to improve proteomic identification of low abundant serum proteins and protein post-translational modifications by active metabolites. In this separation scheme, anionic serum proteins were fractionated by ion exchange followed by size exclusion chromatography. The collected protein

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45

Chou, Chih-Hung, and 周志鴻. "Affinity directed preparation of protein macroinitiator and its application in the study of protein-polymer conjugates." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/32268572916114459932.

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碩士<br>國立中山大學<br>化學系研究所<br>101<br>Protein-polymer conjugates are widely used in medicine, biotechnology, and methods to prepare the bioconjugates. In this research, a chemical method by using synthetic ligand-tosyl-initiator probes is proposed to prepare the protein macroinitiator and then used in the synthesis of protein-polymer conjugates. The ligand- tosyl chemical probes are composed of boronic acid and biotin as affinity ligands, a benzenesulfonyl group as a reactive group and an alkyl halide as the initiator in atom transfer radical polymerization (ATRP). Two proteins of interest, strepta

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46

廖窈萱. "Preparation and Application of Fullerene C60 Immobilized Protein Piezoelectric Bio-Immunosensor." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/85628291422591537262.

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47

Kao, Wei Tse, and 高維澤. "Preparation of cross-linked chitosan nanoparticles and controlled release of protein." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/74845545216217871396.

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48

Wu, Bo-Yi, and 吳柏宜. "Glutathione assisted preparation of gold nanoclusters using minimum amount of protein." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/12050566075775491703.

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碩士<br>國立臺灣大學<br>化學研究所<br>104<br>Bovine serum albumin (BSA) and glutathione (GSH) have been used to prepare gold nanoclusters (BSA/GSH-Au NCs) with excitation/emission wavelengths of 330/650 nm from Au3+ at 70 °C for 30 min. The major roles of BSA and GSH in the preparation of BSA/GSH-Au NCs are acting as capping and reducing agents, respectively. With the assistance of GSH, only 30 μM BSA is required to prepare stable and bright BSA/GSH-Au NCs, leading to low cost and ease in configuration of BSA/GSH-Au NCs. In addition to BSA, lysozyme, ovalbumin, and transferrin have also been used to prepar

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49

Chen, Wei-Ru, and 陳韋如. "Preparation and application of polystyrene and its copolymer for protein adsorption." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/49380355316942807924.

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碩士<br>中原大學<br>化學研究所<br>95<br>The emulsion-free emulsion polymerization technique is used to synthesize the polystyrene that carries different surface voltage. The charge then polystyrene can adsorb BSA protein by electrostatic force under different pH values. The initiator used during the synthesis is the factor to determine the different surface voltages of polystyrene product. When the AIBA initiator is used，the polystyrene particles with diameters averaging 500nm and +19.70mv surface charge are obtained. While the KPS initiator，the polystyrene particles with diameters averaging 586nm and –2

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50

Tasi, Shun-ber, and 蔡尚伯. "Preparation and Characterization of protein-imprinted Poly(ethylene-co-vinyl alcohol)." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/42368612188908277066.

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碩士<br>義守大學<br>材料科學與工程學系碩士班<br>95<br>The concentration of Urea microalbumin was usually use to decision many kinds of disease, like nephrosis, diabetes or high blood pressure in pathology examine of urea. And the concentration range of Urinary microalbumin is located in 0.2mg/ml ~ 0.03 mg/ml in generally. Presently, the decision method of Urea microalbumin Immunoturbidimetric is very useful in hospital. Although this method is accurate to decision, only can measure in hospital was the biggest defect and there will be many limited like effectiveness for a period of time, measure environment for

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