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Journal articles on the topic 'Protein Preparation'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Pęksa, A., and J. Miedzianka. "Amino acid composition of enzymatically hydrolysed potato protein preparations." Czech Journal of Food Sciences 32, No. 3 (2014): 265–72. http://dx.doi.org/10.17221/286/2013-cjfs.

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We determine the effects of the technology of obtaining potato protein preparation and of different variants of enzymatic hydrolysis on the chemical and amino acid compositions of the hydrolysates obtained. Potato protein concentrates obtained through their thermal coagulation in potato juice with calcium chloride, calcium lactate or without salt addition were subjected to enzymatic hydrolysis using two commercial hydrolytic enzymes: endopeptidase (Alcalase) and exopeptidase (Flavourzyme). Chemical (contents of ash, total and coagulable protein) and amino acid compositions of the hydrolysates

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2

Semenova, I. B., and N. A. Mikhailova. "SEROTYPE-INDEPENDENT VACCINES AGAINST PNEUMOCOCCAL INFECTION." Journal of microbiology, epidemiology and immunobiology, no. 4 (August 28, 2016): 76–85. http://dx.doi.org/10.36233/0372-9311-2016-4-76-85.

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Creation of serotype-independent vaccines includes 4 directions - construction of protein vaccines based on recombinant pneumococcus proteins, whole-cell killed or attenuated vaccines, DNA-vaccines and use of Streptococcus pneumoniae as a carrier for polysaccharide and conjugated vaccine preparations. Protein vaccines are the most widely studied. Around 20 proteins are described for pneumococcus - intracellular, associated with cell wall and secreted. The majority of researchers stop at construction of a vaccine preparation including a set of several proteins, protecting from colonization, inv

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Chazot, J. A., and P. G. Strange. "Coupling of D2 dopamine receptors to G-proteins in solubilized preparations of bovine caudate nucleus." Biochemical Journal 281, no. 2 (1992): 369–75. http://dx.doi.org/10.1042/bj2810369.

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1. The coupling of D2 dopamine receptors and G-proteins has been examined in cholate-solubilized preparations of bovine caudate nucleus. 2. No receptor-G-protein coupling could be detected in solubilized preparations obtained in 0.3% cholate, but if this preparation is diluted 5-fold, coupling is re-established. 3. The dilution process was examined, and it was shown that the change in ionic strength was an important factor in modulating the observed receptor-G-protein interaction. 4. Ionic strength was shown, however, not to be the primary determinant of receptor-G-protein coupling. This is li

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Shaginova, L. O., I. V. Krylova, T. F. Demianenko, and M. L. Domoroshchenkova. "Study of a process of obtaining of protein preparation from sunflower seeds for application in food industry." New Technologies 17, no. 3 (2021): 41–50. http://dx.doi.org/10.47370/2072-0920-2021-17-3-41-50.

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The article deals with a study of processing methods of obtaining protein preparations from sunflower seeds. For obtaining of food-grade protein preparations from the defatted sunflower kernels phenolic compounds were additionally removed. Modern Russian and foreign literature sources devoted to the isolation of phenolic compounds from vegetable protein raw materials were analyzed. The objective of obtaining a light-colored food-grade protein preparation from sunflower seeds was solved through developing of a multi-stage extraction process with removal of various groups of non-protein compound

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Cohen, D. C., and P. T. Doyle. "Effect of sample preparation on in situ estimates of protein degradability for white clover herbages." Australian Journal of Experimental Agriculture 41, no. 5 (2001): 619. http://dx.doi.org/10.1071/ea00168.

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The amount of crude protein in white clover solubilised during ingestion by cows was determined by collecting swallowed boluses in rumen fistulated cows. Six experiments were then conducted to determine the effects of sample preparation on in situ estimates of rumen degradability of crude protein in white clover (Trifolium repens L.) herbages. Different methods of preparation were compared with chopped fresh herbage, a recommended method when this type of feed is incubatedin situ. Samples of white clover were either: (i) chopped fresh to 10 mm lengths (FRS), (ii) frozen, freeze-dried and groun

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6

Pahud, Jean‐Jacques, Julio César Monti, and Rolf Jost. "Allergenicity of Whey Protein." Journal of Pediatric Gastroenterology and Nutrition 4, no. 3 (1985): 408–13. http://dx.doi.org/10.1002/j.1536-4801.1985.tb08871.x.

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Enzymatic in vitro hydrolysis was evaluated as a possible treatment to abolish the allergenicity of whey proteins in view of their use in infant formulas. Guinea pigs without prior immunological contact (including fetal life) with cow'S milk were fed various preparations of cow'S milk proteins. Oral exposure to milk or untreated whey protein led to anaphylactic sensitization of the animals. In contrast, trypsin‐hydrolyzed whey protein and a peptide preparation produced from the tryptic hydrolysate by ultrafiltration were devoid of sensitizing capacity by the oral route. The hydrolysate (crude

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7

Toru-Delbauffe, D., M. Pierre, J. Osty, F. Chantoux, and J. Francon. "Properties of neurofilament protein kinase." Biochemical Journal 235, no. 1 (1986): 283–89. http://dx.doi.org/10.1042/bj2350283.

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Neurofilament (NF) protein kinase, partially purified from NF preparations [Toru-Delbauffe & Pierre (1983) FEBS Lett. 162, 230-234], was found to be distinct from both the casein kinase present in NFs and the cyclic AMP-dependent protein kinase which is able to phosphorylate NFs. NF-kinase phosphorylated the three NF protein components. The amount of phosphate incorporated per molecule was higher for NF 200 than for NF 145 and NF 68. Other proteins present in the NF preparations were also used as NF-kinase substrates. Two of them might correspond to the myelin basic proteins with Mr values

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8

Meyer,, Kevin. "Automating Protein Sample Preparation." Genetic Engineering & Biotechnology News 31, no. 1 (2011): 28–29. http://dx.doi.org/10.1089/gen.31.1.14.

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9

Schotters, S. B., J. H. McBride, D. O. Rodgerson, S. Higgins, and M. Pisa. "Standardization for four protein analytes with the Behring Nephelometer." Clinical Chemistry 34, no. 9 (1988): 1870–72. http://dx.doi.org/10.1093/clinchem/34.9.1868.

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Abstract Owing to the generally higher values observed in the initial establishment of immunoglobulins (Ig) G, A, and M assays with the Behring Nephelometer, we elected to verify five commercial protein calibrators. This initial verification was performed by standardizing the Behring Nephelometer with the World Health Organization (WHO) International Reference Preparation for Human Serum Immunoglobulins G, A, and M. The instrument was also standardized for immunoglobulins and transferrin with use of the Reference Preparation for Serum Proteins (RPSP II). Analytical recoveries of the commercial

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10

Cheng, Hao-Tsai, Sen-Yung Hsieh, Chang-Mu Sung, Betty Chien-Jung Pai, Nai-Jen Liu, and Carl PC Chen. "Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis." BioMed Research International 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/5185317.

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Aims. Bile is an important body fluid which assists in the digestion of fat and excretion of endogenous and exogenous compounds. In the present study, an improved sample preparation for human bile was established.Methods and Material. The method involved acetone precipitation followed by protein extraction using commercially available 2D Clean-Up kit. The effectiveness was evaluated by 2-dimensional electrophoresis (2DE) profiling quality, including number of protein spots and spot distribution.Results. The total protein of bile fluid in benign biliary disorders was 0.797 ± 0.465 μg/μL. The sa

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11

Pang, Eric, Steve Clarke, and Jose Rodriguez. "Automated vitrification with self-wicking grids may limit potential preferred orientation of CRL5-PCMTD1, a putative cullin-RING ligase, during cryoEM sample preparation." Structural Dynamics 12, no. 2_Supplement (2025): A369. https://doi.org/10.1063/4.0000675.

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Protein carboxyl methyltransferase domain-containing protein 1 (PCMTD1) may be the substrate adaptor protein of a potential E3 ubiquitin ligase that ubiquitylates proteins harboring isoaspartyl damage for proteasomal degradation. PCMTD1 is able to multimerize with cullin-RING ligase (CRL) components to form the putative CRL: CRL5-PCMTD1. We set out to structurally characterize this novel E3 ubiquitin ligase to elucidate its potential role in preventing the accumulation of age-damaged proteins. Because CRLs are known to function dynamically to facilitate ubiquitylation of substrates, we pursued

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12

Dosemeci, A., and H. C. Pant. "Association of cyclic-AMP-dependent protein kinase with neurofilaments." Biochemical Journal 282, no. 2 (1992): 477–81. http://dx.doi.org/10.1042/bj2820477.

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Neurofilament preparations isolated from bovine spinal cord contain cyclic-AMP-dependent protein kinase (PKA) activity. Treatment of this preparation with cyclic AMP, to dissociate the regulatory subunit of the kinase from the catalytic subunit, resulted in retention of the kinase activity but loss of cyclic AMP regulation. This suggests that PKA is associated via its catalytic subunit with the neurofilament preparation. The association of exogenous PKA from bovine heart with the neurofilament preparation and with neurofilaments reconstituted from purified neurofilament proteins was also inves

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13

Żulewska, Justyna, Maria Baranowska, Marika Magdalena Bielecka, et al. "Effect of Fortification with High-Milk-Protein Preparations on Yogurt Quality." Foods 14, no. 1 (2025): 80. https://doi.org/10.3390/foods14010080.

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Protein-enriched yogurts have become increasingly popular among consumers seeking to boost their daily protein intake. The incorporation of milk proteins and protein preparations in yogurt production not only enhances nutritional value but also improves texture, viscosity, and overall sensory properties—key factors that influence consumer acceptance. The main objective of this study was to evaluate the influence of casein and whey protein preparations on the physicochemical properties, viability of lactic acid bacteria, and sensory attributes of yogurts. Yogurts were enriched with 2% (w/w) pro

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14

Webster, Paul. "Preparation of Protein a Gold." Microscopy Today 5, no. 5 (1997): 12–13. http://dx.doi.org/10.1017/s1551929500061563.

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Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years, colloidal gold particles have become a useful tool in microscopy for staining tissues and sections. Colloidal gold particles are especially useful for biological electron microscopy, Some of the reasons why are listed below.*Homogeneous preparations of particles varying in size from 3μm to 20μm can be easily prepared.*Colloidal gold suspensions are inexpensive to prepare. Most proteins can be easily coupled to colloidal gold particles.*Most proteins can be easily coup

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15

Moorman, Veronica R., and Alexandra M. Brayton. "Identification of individual components of a commercial wheat germ acid phosphatase preparation." PLOS ONE 16, no. 3 (2021): e0248717. http://dx.doi.org/10.1371/journal.pone.0248717.

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Wheat germ acid phosphatase (WGAP) is a commercial preparation of partially purified protein commonly used in laboratory settings for non-specific enzymatic dephosphorylation. It is known that these preparations contain multiple phosphatase isozymes and are still relatively crude. This study therefore aimed to identify the protein components of a commercial preparation of wheat germ acid phosphatase using mass spectroscopy and comparative genomics. After one post-purchase purification step, the most prevalent fifteen proteins in the mixture included heat shock proteins, beta-amylases, glucoser

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16

Melnikova, Elena, Ekaterina Stanislavskaya, and Evgeniy Korotkov. "PREPARATION AND USE OF WHEY PROTEIN MICROPARTICULATE IN SYNBIOTIC DRINK TECHNOLOGY." Foods and Raw Materials 3, no. 2 (2015): 96–104. http://dx.doi.org/10.12737/13125.

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17

Boonen, Marielle, Isabelle Hamer, Muriel Boussac, et al. "Intracellular localization of p40, a protein identified in a preparation of lysosomal membranes." Biochemical Journal 395, no. 1 (2006): 39–47. http://dx.doi.org/10.1042/bj20051647.

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Unlike lysosomal soluble proteins, few lysosomal membrane proteins have been identified. Rat liver lysosomes were purified by centrifugation on a Nycodenz density gradient. The most hydrophobic proteins were extracted from the lysosome membrane preparation and were identified by MS. We focused our attention on a protein of approx. 40 kDa, p40, which contains seven to ten putative transmembrane domains and four lysosomal consensus sorting motifs in its sequence. Knowing that preparations of lysosomes obtained by centrifugation always contain contaminant membranes, we combined biochemical and mo

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18

Lehtonen, S. I., B. Taskinen, E. Ojala, et al. "Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning." Protein Engineering Design and Selection 28, no. 1 (2014): 23–28. http://dx.doi.org/10.1093/protein/gzu050.

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19

Saunier, Bertrand, Miriam Triyatni, Luca Ulianich, Padma Maruvada, Paul Yen, and Leonard D. Kohn. "Role of the Asialoglycoprotein Receptor in Binding and Entry of Hepatitis C Virus Structural Proteins in Cultured Human Hepatocytes." Journal of Virology 77, no. 1 (2003): 546–59. http://dx.doi.org/10.1128/jvi.77.1.546-559.2003.

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ABSTRACT We used a baculovirus-based system to prepare structural proteins of hepatitis C virus (HCV) genotype 1a. Binding of this preparation to cultured human hepatic cells was both dose dependent and saturable. This binding was decreased by calcium depletion and was partially prevented by ligands of the asialoglycoprotein receptor (ASGP-R), thyroglobulin, asialothyroglobulin, and antibody against a peptide in the carbohydrate recognition domain of ASGP-R but not preimmune antibody. Uptake by hepatocytes was observed with both radiolabeled and dye-labeled HCV structural proteins. With hepato

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20

Araújo, Mário Jorge, Maria Lígia Sousa, Aldo Barreiro Felpeto, et al. "Comparison of Sample Preparation Methods for Shotgun Proteomic Studies in Aquaculture Species." Proteomes 9, no. 4 (2021): 46. http://dx.doi.org/10.3390/proteomes9040046.

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Proteomics has been recently introduced in aquaculture research, and more methodological studies are needed to improve the quality of proteomics studies. Therefore, this work aims to compare three sample preparation methods for shotgun LC–MS/MS proteomics using tissues of two aquaculture species: liver of turbot Scophthalmus maximus and hepatopancreas of Mediterranean mussel Mytilus galloprovincialis. We compared the three most common sample preparation workflows for shotgun analysis: filter-aided sample preparation (FASP), suspension-trapping (S-Trap), and solid-phase-enhanced sample preparat

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21

Feichtinger, Annika, and Elke Scholten. "Preparation of Protein Oleogels: Effect on Structure and Functionality." Foods 9, no. 12 (2020): 1745. http://dx.doi.org/10.3390/foods9121745.

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Among available structuring agents that have been used to provide solid properties to liquid oils, protein is a more recent candidate. Due to their nutritional value and high consumer acceptance, proteins are of special interest for the preparation of edible oleogels as an alternative for solid fats. Whereas the field of protein oleogelation is still rather new and just starts unfolding, several preparation methods have been demonstrated to be suitable for protein oleogel preparation. However, there is limited knowledge regarding the link between microstructural properties of the gels and macr

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22

Beretov, Julia, Valerie C. Wasinger, Peter Schwartz, Peter H. Graham, and Yong Li. "A Standardized and Reproducible Urine Preparation Protocol for Cancer Biomarkers Discovery." Biomarkers in Cancer 6 (January 2014): BIC.S17991. http://dx.doi.org/10.4137/bic.s17991.

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A suitable and standardized protein purification technique is essential to maintain consistency and to allow data comparison between proteomic studies for urine biomarker discovery. Ultimately, efforts should be made to standardize urine preparation protocols. The aim of this study was to develop an optimal analytical protocol to achieve maximal protein yield and to ensure that this method was applicable to examine urine protein patterns that distinguish disease and disease-free states. In this pilot study, we compared seven different urine sample preparation methods to remove salts, and to pr

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23

Suslick, Kenneth S., Kenneth J. Kolbeck, Gregory S. Kufner, and Gregory W. Szewczyk. "Sonochemical preparation of protein microspheres." Journal of the Acoustical Society of America 103, no. 5 (1998): 2923. http://dx.doi.org/10.1121/1.422125.

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24

González-García, Estefanía, María Luisa Marina, and María Concepción García. "Nanomaterials in Protein Sample Preparation." Separation & Purification Reviews 49, no. 3 (2019): 229–64. http://dx.doi.org/10.1080/15422119.2019.1581216.

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25

Jaganathan, Maheshkumar, D. Madhumitha, and A. Dhathathreyan. "Protein microcapsules: Preparation and applications." Advances in Colloid and Interface Science 209 (July 2014): 1–7. http://dx.doi.org/10.1016/j.cis.2013.12.004.

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26

Brynda, Eduard, and Milan Houska. "Preparation of organized protein multilayers." Macromolecular Rapid Communications 19, no. 4 (1998): 173–76. http://dx.doi.org/10.1002/(sici)1521-3927(19980401)19:4<173::aid-marc173>3.0.co;2-s.

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27

Krasnoshtanova, Alla Al'bertovna, and Leonid Viktorovich Shul'ts. "PREPARATION AND EVALUATION OF THE FUNCTIONAL PROPERTIES OF PROTEIN ISOLATES AND HY-DROLYSATES FROM PLANT RAW MATERIALS." chemistry of plant raw material, no. 4 (December 15, 2022): 299–309. http://dx.doi.org/10.14258/jcprm.20220410952.

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Animal protein plays a key role in the human diet as the most balanced amino acid composition; however, its consumption often causes allergic reactions. Plant protein serves as a substitute for animal protein. The most promising sources of plant protein are the seeds of cereals, pulses, oilseeds and cereals. Research aim: selection of conditions for obtaining protein isolates and enzymatic hydrolysates having the desired functional properties from different types of vegetable raw materials.Pea, corn and oat flour LLC "FavoritT"; linseed flour LLC NGO "Compass Health". Enzyme preparations: chym

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28

Lv, Shi-Wen, Lei-Yu He, and Li-Hui Sun. "Effect of different stabilisation treatments on preparation and functional properties of rice bran proteins." Czech Journal of Food Sciences 36, No. 1 (2018): 57–65. http://dx.doi.org/10.17221/100/2017-cjfs.

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The stabilisation treatments of rice bran were performed using microwave heating (100% power, 1–2 min) and dry heating (120°C, 10–20 min), respectively, and then protein was prepared by alkaline extraction (pH 9.5) and acid precipitation (pH 4.5). Stabilisation treatments resulted in a decrease in the protein yield, but an increase in the protein purity. Heat-stabilisation was effective in inhibiting the rancidity of rice bran, and microwave heating was more effective than dry heating. The functional properties of proteins such as the emulsifying properties and oil holding capacity were impro

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29

Klimenko, Vladimir, and Svetlana Malyarenko. "PREPARATION OF QUALITATIVE FERMENTED FEEDS FROM LEGUME GRASSES." Adaptive Fodder Production 2021, no. 4 (2022): 67–76. http://dx.doi.org/10.33814/afp-2222-5366-2021-4-67-76.

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The current study presents the results on evaluation the preservative efficiency of domestic biological preparations, based on enzymes, at the ensiling and haylage preparation of high-protein leguminous grasses. High-yielding varieties of alfalfa, red clover and fodder galega (Galega orientalis Lam.), that were developed in Williams Research Center of Forage Production & Agroecology were used as plant raw material. Plants were harvested in the optimal vegetation phases. The effect of the new biological preparations on safety and quality of fermented feeds in terms of organoleptic and bioch

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30

Heikkinen, Harri A., Sofia M. Backlund, and Hideo Iwaï. "NMR Structure Determinations of Small Proteins Using only One Fractionally 20% 13C- and Uniformly 100% 15N-Labeled Sample." Molecules 26, no. 3 (2021): 747. http://dx.doi.org/10.3390/molecules26030747.

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Uniformly 13C- and 15N-labeled samples ensure fast and reliable nuclear magnetic resonance (NMR) assignments of proteins and are commonly used for structure elucidation by NMR. However, the preparation of uniformly labeled samples is a labor-intensive and expensive step. Reducing the portion of 13C-labeled glucose by a factor of five using a fractional 20% 13C- and 100% 15N-labeling scheme could lower the total chemical costs, yet retaining sufficient structural information of uniformly [13C, 15N]-labeled sample as a result of the improved sensitivity of NMR instruments. Moreover, fractional 1

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31

Uyanga, Sambuudorzhiyn, Ganzhuurzhav Oyuun, Tamara Lefler, and Evgeniya Turitsyna. "COMPARATIVE CHARACTERISTICS OF PROTEIN PRODUCTS OBTAINED FROM THE TESTES OF SLAUGHTERED ANIMALS." Bulletin of KSAU, no. 8 (January 29, 2025): 91–98. https://doi.org/10.36718/1819-4036-2023-8-91-98.

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The paper gives a comparative description of protein products obtained from the testes of a bull and a stallion to determine the possibility of using these drugs as a food supplement. The preparations were obtained by the method of sublimation and low-temperature (50–55 °C) drying from endocrine-enzymatic raw materials obtained during slaughter of cattle (Ulaanbaatar, Mongolia). The chemical, amino acid and miner-al composition of preparations was studied. Both products are characterized by a high protein content - from 69.6 to 74.1 %. In the preparation from the testes of the stallion, the pr

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32

Dong Y Qi, G., Z. Yang, H. Wang, S. Wang, and G. Chen. "Preparation, separation and antioxidant properties of hydrolysates derived from Grifola frondosa protein." Czech Journal of Food Sciences 33, No. 6 (2016): 500–506. http://dx.doi.org/10.17221/197/2015-cjfs.

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33

Hinchman, Susan K., and Sheldon M. Schuster. "Overproduction, preparation of monoclonal antibodies and purification of E.coli asparagine synthetase A." "Protein Engineering, Design and Selection" 5, no. 3 (1992): 279–83. http://dx.doi.org/10.1093/protein/5.3.279.

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34

O'Rourke, F., G. B. Zavoico, and M. B. Feinstein. "Release of Ca2+ by inositol 1,4,5-trisphosphate in platelet membrane vesicles is not dependent on cyclic AMP-dependent protein kinase." Biochemical Journal 257, no. 3 (1989): 715–21. http://dx.doi.org/10.1042/bj2570715.

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In contrast with previous reports, it was found that membrane-protein phosphorylation by the catalytic subunit (CS) of cyclic AMP-dependent protein kinase had no effect on Ca2+ uptake into platelet membrane vesicles or on subsequent Ca2+ release by inositol 1,4,5-trisphosphate (IP3). Furthermore, IP-20, a highly potent synthetic peptide inhibitor of CS, which totally abolished membrane protein phosphorylation by endogenous or exogenous CS, also had no effect on either Ca2+ uptake or release by IP3. Commercial preparations of protein kinase inhibitor protein (PKI) usually had no effect, but one

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35

Kurcheva, S. A., D. A. Kovalev, D. G. Ponomarenko, et al. "Qualitative Indicators of Experimental Brucellosis Antigen Preparations Designed for Cellular Tests in vitro." Problems of Particularly Dangerous Infections, no. 3 (October 22, 2020): 83–88. http://dx.doi.org/10.21055/0370-1069-2020-3-83-88.

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In order to develop the most diagnostically informative methods for carrying out antigen-stimulated cellular tests in vitro a careful selection of stimulating agent (antigen) is required, possessing an adequate activating potential and providing specificity of the reaction.Objective of the study was to identify the qualitative indicators of experimental batches of brucellosis antigen preparations designed for cellular tests in vitro.Materials and methods. Initially we produced antigen complexes of brucellosis microbe on the basis of the vaccine strains of three epidemically significant Brucell

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Homonylo-McGavin, Mary K., Song F. Lee, and George H. Bowden. "Subcellular localization of the Streptococcus mutans P1 protein C terminus." Canadian Journal of Microbiology 45, no. 6 (1999): 536–39. http://dx.doi.org/10.1139/w99-037.

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To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal antibody preparation directed against the P1 C-terminal 144 amino acids (P1COOH). P1 protein was detected in cell walls but not the membrane purified from S. mutans cells by the monoclonal antibody. In contrast, P1 protein was not detected in the same cell wall preparation using

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37

Du, Yan, Fusheng Chen, Chen Chen, and Kunlun Liu. "Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation." Royal Society Open Science 7, no. 10 (2020): 201147. http://dx.doi.org/10.1098/rsos.201147.

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To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in ma

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38

Nomani, Alireza, Hamed Nosrati, Hamidreza Manjili, Leila Khesalpour, and Hossein Danafar. "Preparation and Characterization of Copolymeric Polymersomes for Protein Delivery." Drug Research 67, no. 08 (2017): 458–65. http://dx.doi.org/10.1055/s-0043-106051.

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AbstractBiodegradable copolymeric polymersomes have been used for controlled drug delivery of proteins. These polymersomes important areas to overcome formulation associated problems of the proteins. The aim of this study was to develop polymersomes using biodegradable copolymers for delivery of bovine serum albumin (BSA) as a model protein. Encapsulated BSA by mPEG-PCL polymersomes led to formation of BSA-loaded mPEG-PCL polymersomes. The polymersomes synthesized with the protein-polymer ratio of 1:4 at 15 000 rpm gave maximum loading, minimum polydispersion with maximally sustained protein r

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Tkáčiková, Soňa, Ivan Talian, and Ján Sabo. "Optimisation of urine sample preparation for shotgun proteomics." Open Chemistry 18, no. 1 (2020): 850–56. http://dx.doi.org/10.1515/chem-2020-0150.

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AbstractUrine reflects the renal function and urinary and kidney systems, but it may also reflect the presence of cancer in other parts of the body. Urine also has potential for providing prognostic information during therapeutic treatments thanks to non-invasive monitoring. A quick and reproducible protein purification procedure is essential to allow data comparison between proteomic studies in urine biomarker discovery. The article describes a simple, reproducible and cheap sample preparation procedure with a maximum protein yield (400 µg) obtained from only 10 mL of urine utilising cut-off

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40

Van Patten, S. M., A. Hotz, V. Kinzel, and D. A. Walsh. "The inhibitor protein of the cyclic AMP-dependent protein kinase-catalytic subunit interaction. Composition of multiple complexes." Biochemical Journal 256, no. 3 (1988): 785–89. http://dx.doi.org/10.1042/bj2560785.

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It has been previously demonstrated that the combination of pure preparations of the inhibitor protein of the cyclic AMP-dependent protein kinase and the catalytic subunit of this enzyme resulted in the formation of multiple complexes [Van Patten, Fletcher & Walsh (1986) J. Biol. Chem. 261, 5514-5523]. In the present study it is demonstrated that these multiple species occur because the bovine heart protein kinase preparation contains multiple forms of catalytic subunit [Kinzel, Hotz, König, Gagelmann, Pyerin, Reed, Köbler, Hofmann, Obst, Gensheimer, Goldblatt & Shaltiel (1987) Arch. B

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Liu, Yue Hui, Jun Wang, Xin Chao Yang, Na Xin Sun, and Li Hua Xu. "Preparation of Antibodies against Maize Inbred Lines Storage Proteins." Advanced Materials Research 781-784 (September 2013): 1717–22. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.1717.

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A convenient way of producing effective antibody against maize seed storage proteins is present.The protein patterns of maize hybrid seed Ludan50 have two special protein bands with Polyacrylamide gel electrophoresis (PAGE) which was corresponding to its parent inbred lines Luyuan92 and Qi319. We purified the two proteins respectively by acid-PAGE and then immunized rabbit and laying hens, obtained the antiserums and yolk antibodies. Both antiserums and yolk antibodies have good specificity against the antigens, except that the yolk antibody has a higher titer. We also measured its specificity

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Rosenkrands, Ida, Karin Weldingh, Pernille Ravn, et al. "Differential T-Cell Recognition of Native and Recombinant Mycobacterium tuberculosis GroES." Infection and Immunity 67, no. 11 (1999): 5552–58. http://dx.doi.org/10.1128/iai.67.11.5552-5558.1999.

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ABSTRACT Mycobacterium tuberculosis GroES was purified from culture filtrate, and its identity was confirmed by immunoblot analysis and N-terminal sequencing. Comparing the immunological recognition of native and recombinant GroES, we found that whereas native GroES elicited a strong proliferative response and release of gamma interferon-γ by peripheral blood mononuclear cells from healthy tuberculin reactors, the recombinant protein failed to do so. The same difference in immunological recognition was observed in a mouse model of TB infection. Both the native and recombinant preparations were

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Dr., Rajaganapathy Kaliyaperumal* Selvakumar R. Diana L. Aswin A. Keerthivasan K. M. Srinivasan. R. "Advancing In-Silico Drug Discovery: A Comprehensive Strategy for Homology Modeling, Protein Preparation, and Ligand Optimization in Molecular Docking." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 4338–60. https://doi.org/10.5281/zenodo.15517725.

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Molecular docking is a pivotal computational approach in modern drug discovery, enabling the prediction of interactions between ligands and target proteins. However, the accuracy of docking outcomes depends on the quality of the input structures, necessitating a robust workflow that integrates homology modeling, protein preparation, and ligand preparation. This review aims to explore the current strategies in homology modeling, protein optimization, and ligand preparation to enhance molecular docking accuracy. It highlights key tools, methodologies, and recent advancements in in-silico modelin

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Whitlow, Marc, David Filpula, Michele L. Rollence, Sheau-Line Feng, and James F. Wood. "Multivalent Fvs: characterization of single-chain Fv oligomers and preparation of a bispecific Fv." "Protein Engineering, Design and Selection" 7, no. 8 (1994): 1017–26. http://dx.doi.org/10.1093/protein/7.8.1017.

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Syrkina, M. S., D. A. Shirokov, M. A. Rubtsov, E. L. Kadyrova, V. P. Veiko, and V. A. Manuvera. "Preparation and functional evaluation of RGD-modified streptavidin targeting to integrin-expressing melanoma cells." Protein Engineering Design and Selection 26, no. 2 (2012): 143–50. http://dx.doi.org/10.1093/protein/gzs076.

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Savoy, Luc-Alain, Pierre Vuagnat, Keith Rose, John Halstrøm, Klavs H. Jørgensen, and Karoly Kovacs. "The preparation of porcine (Tyr-A14-2H) insulin and its characterization by mass spectrometry." "Protein Engineering, Design and Selection" 2, no. 4 (1988): 313–15. http://dx.doi.org/10.1093/protein/2.4.313.

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Raharjo, Tri Joko, Rusmiati Suprihatin, and Deni Pranowo. "THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM." Indonesian Journal of Chemistry 7, no. 3 (2010): 337–41. http://dx.doi.org/10.22146/ijc.21680.

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A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (

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Li, Ci Li, and Bing Han. "The Preparation of Hydrolyzed Carp Protein." Advanced Materials Research 183-185 (January 2011): 343–46. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.343.

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The carp was used to prepare fish protein hydrolysate in the article. The results demonstrated that the optimum conditions for preparation of hydrolyzed fish protein with Neutrase by orthogonal test are as follows: the temperature is 50°C, the enzyme content is 2.0%, the pH value is 7.5, the hydrolyzed time is 5 hours, the weight ratio of meat to water is 1:6. Under the conditions, the recovery of hydrolyzed protein was 94.28%.

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Yamada, Masanori, Sakura Morimitsu, Eiji Hosono, and Tetsuya Yamada. "Preparation of bioplastic using soy protein." International Journal of Biological Macromolecules 149 (April 2020): 1077–83. http://dx.doi.org/10.1016/j.ijbiomac.2020.02.025.

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MacBeath, Gavin. "Protein Arrays: Preparation of Microscope Slides." Cold Spring Harbor Protocols 2007, no. 3 (2007): pdb.prot4629. http://dx.doi.org/10.1101/pdb.prot4629.

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