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Journal articles on the topic 'Protein titration'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Chen, Po-chia, Pawel Masiewicz, Kathryn Perez, and Janosch Hennig. "Structure-based screening of binding affinities via small-angle X-ray scattering." IUCrJ 7, no. 4 (2020): 644–55. http://dx.doi.org/10.1107/s2052252520004169.

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Protein–protein and protein–ligand interactions often involve conformational changes or structural rearrangements that can be quantified by solution small-angle X-ray scattering (SAXS). These scattering intensity measurements reveal structural details of the bound complex, the number of species involved and, additionally, the strength of interactions if carried out as a titration. Although a core part of structural biology workflows, SAXS-based titrations are not commonly used in drug discovery contexts. This is because prior knowledge of expected sample requirements, throughput and prediction

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2

HE, Qing-Yu, Anne B. MASON, and Robert C. WOODWORTH. "Spectrophotometric titration with cobalt(III) for the determination of accurate absorption coefficients of transferrins." Biochemical Journal 318, no. 1 (1996): 145–48. http://dx.doi.org/10.1042/bj3180145.

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A rapid and sensitive technique, involving difference spectral titration with cobalt(III), to measure the ϵ values of chicken ovotransferrin, human serum transferrin, the N-lobe of human transferrin and several single point mutants is reported. The resulting ϵ values were compared with the values calculated from the equation proposed by Pace, Vajdos, Fee, Grimsley and Gray [(1995) Protein Sci. 4, 2411–2423]. The titrations with cobalt feature sharp break-points and do not destroy the protein samples. The choice of buffer was found to be important, depending on the metal-binding avidity of the

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3

Wang, Xiaohan, Kai Zheng, Yi Si, Xuhong Guo, and Yisheng Xu. "Protein–Polyelectrolyte Interaction: Thermodynamic Analysis Based on the Titration Method †." Polymers 11, no. 1 (2019): 82. http://dx.doi.org/10.3390/polym11010082.

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This review discussed the mechanisms including theories and binding stages concerning the protein–polyelectrolyte (PE) interaction, as well as the applications for both complexation and coacervation states of protein–PE pairs. In particular, this review focused on the applications of titration techniques, that is, turbidimetric titration and isothermal titration calorimetry (ITC), in understanding the protein–PE binding process. To be specific, by providing thermodynamic information such as pHc, pHφ, binding constant, entropy, and enthalpy change, titration techniques could shed light on the b

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Pierce, Michael M., C. S. Raman, and Barry T. Nall. "Isothermal Titration Calorimetry of Protein–Protein Interactions." Methods 19, no. 2 (1999): 213–21. http://dx.doi.org/10.1006/meth.1999.0852.

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5

Lin, Yanchun, and Michael L. Gross. "Mass Spectrometry-Based Structural Proteomics for Metal Ion/Protein Binding Studies." Biomolecules 12, no. 1 (2022): 135. http://dx.doi.org/10.3390/biom12010135.

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Metal ions are critical for the biological and physiological functions of many proteins. Mass spectrometry (MS)-based structural proteomics is an ever-growing field that has been adopted to study protein and metal ion interactions. Native MS offers information on metal binding and its stoichiometry. Footprinting approaches coupled with MS, including hydrogen/deuterium exchange (HDX), “fast photochemical oxidation of proteins” (FPOP) and targeted amino-acid labeling, identify binding sites and regions undergoing conformational changes. MS-based titration methods, including “protein–ligand inter

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Maiolo, Daniele, Paolo Bergese, Eugene Mahon, Kenneth A. Dawson, and Marco P. Monopoli. "Surfactant Titration of Nanoparticle–Protein Corona." Analytical Chemistry 86, no. 24 (2014): 12055–63. http://dx.doi.org/10.1021/ac5027176.

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7

Berisio, R., V. S. Lamzin, F. Sica, K. S. Wilson, A. Zagari, and L. Mazzarella. "Protein titration in the crystal state." Journal of Molecular Biology 292, no. 4 (1999): 845–54. http://dx.doi.org/10.1006/jmbi.1999.3093.

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8

Karshikoff, Andrej. "A simple algorithm for the calculation of multiple site titration curves." "Protein Engineering, Design and Selection" 8, no. 3 (1995): 243–48. http://dx.doi.org/10.1093/protein/8.3.243.

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9

Ngo, Ha-Thanh, and Thomas Bechtold. "Analysis of the Fibroin Solution State in Calcium Chloride/Water/Ethanol for Improved Understanding of the Regeneration Process." Fibres and Textiles in Eastern Europe 26, no. 6(132) (2018): 43–50. http://dx.doi.org/10.5604/01.3001.0012.5174.

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Shaping of fibroin protein from Bombyx mori from calcium chloride/water/ethanol solution is of high interest for the manufacturing of biocompatible structures. Potentiometric titration experiments of the dissolved state permit new insight into the solution state of fibroin as a basis for improved regeneration. Titration experiments and infrared spectroscopy of the solution state support the model of an ion-rich hydration layer and interaction of the solvent with charged and polar groups of the fibroin, rather than through formation of defined calcium complexes. The potentiometric titration cur

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Hicks, Pamela S., Christopher L. Andrews, and David G. Bear. "Quantitative analysis of the cooperativity of protein-nucleic acid interactions by electron microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 308–9. http://dx.doi.org/10.1017/s042482010014316x.

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It is common for sing1e-stranded nucleic acid finding proteins to bind polynucleotides cooperatively. The parameter ω is used to describe the component of the association binding constant that is due to cooperative interactions between protein molecules on the polynucleotide lattice; ω is 1 for noncooperative binding, while ω can be as high as 103-104 for highly cooperative proteins such as bacteriophage T4 gene 32 protein. In the past, to has been determined by computer fitting of spectroscopic titration data. It has been suggested that electrom microscopy would provide a more direct method f

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11

Nuebel, Matthew, Jonathan G. Leung, Christopher Hughes, and Ian McGrane. "Evaluation of major adverse events of clozapine based on accordance to an international titration guideline." Mental Health Clinician 14, no. 3 (2024): 204–11. http://dx.doi.org/10.9740/mhc.2024.06.204.

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Introduction Clozapine is the only antipsychotic approved for treatment-resistant schizophrenia, but without appropriate monitoring, it can be associated with potentially fatal outcomes. An International Adult Clozapine Titration Guideline categorizes patients into normal or slow metabolizers. Categorization provides clozapine titration schedules and recommends regular c-reactive protein (CRP) and clozapine concentration monitoring to reduce the risk of adverse drug reactions (ADRs). The impact of the guideline on clozapine ADRs has not been evaluated. Methods A retrospective chart review asse

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12

Zadok, Uri, Alfred E. Asato, and Mordechai Sheves. "Titration of the Bacteriorhodopsin Schiff Base Involves Titration of an Additional Protein Residue†." Biochemistry 44, no. 23 (2005): 8479–85. http://dx.doi.org/10.1021/bi0500978.

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13

Beaumont, Christelle, Cristiana Stuani, Ming-Yuan Chou, et al. "hnRNP A1 induces aberrantCFTRexon 9 splicing via a newly discovered ESS element." Life Science Alliance 8, no. 9 (2025): e202402720. https://doi.org/10.26508/lsa.202402720.

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RNA–protein interactions play a key role in the aberrant splicing ofCFTRexon 9. Exon 9 skipping leads to the production of a nonfunctional chloride channel associated with severe forms of cystic fibrosis. The missplicing depends on TDP-43 binding to an extended UG-rich binding site upstream ofCFTRexon 9 3′ splicing site (3′ss) and is associated with concomitant hnRNP A1 recruitment. Although TDP-43 is the dominant inhibitor of exon 9 inclusion, the role of hnRNP A1, a protein with two RNA recognition motifs, remained unclear. In this work, we have studied the interaction between hnRNP A1 and t

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14

Zhou, Yaoqi. "Salt Effects on Protein Titration and Binding." Journal of Physical Chemistry B 102, no. 51 (1998): 10615–21. http://dx.doi.org/10.1021/jp982542x.

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15

Draczkowski, Piotr, Dariusz Matosiuk, and Krzysztof Jozwiak. "Isothermal titration calorimetry in membrane protein research." Journal of Pharmaceutical and Biomedical Analysis 87 (January 2014): 313–25. http://dx.doi.org/10.1016/j.jpba.2013.09.003.

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16

De Jong, Paul E., Gerjan Navis, and Dick de Zeeuw. "Renoprotective therapy: titration against urinary protein excretion." Lancet 354, no. 9176 (1999): 352–53. http://dx.doi.org/10.1016/s0140-6736(99)90122-8.

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17

Zähner, Dagmar, Remedios Ramirez, and Willy J. Malaisse. "Non-enzymatic protein glycosylation: back-titration assay." Diabetes Research and Clinical Practice 8, no. 1 (1990): 61–68. http://dx.doi.org/10.1016/0168-8227(90)90097-d.

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18

Moon, Hyuk-Jin, Hyun-Jung Kim, Dong-Hyun Lee, Seo-Yeong Mun, and Soo-Dong Woo. "Rapid and Economic Baculovirus Titer Determination Using a Novel Transgenic Sf9-QE Cell Line." Insects 16, no. 4 (2025): 426. https://doi.org/10.3390/insects16040426.

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A baculovirus expression system (BES) for the production of recombinant proteins requires rapid and easy virus titer determination. In this study, a novel direct titration method was developed using a novel Sf9-QE cell line to easily and economically determine virus titers in a short time. This direct titration method can determine virus titers by directly counting the initially infected cells. This method requires the rapid identification of the initial virus-infected cells. The genome of Sf9-QE cells, which fluoresce upon virus infection, was found to contain at least seven copy numbers of t

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19

Wang, Haiyan, Fenglan Zhang, Jin Cao, Qingsheng Zhang, and Zhirong Chen. "Comparison of Chromatographic and Titrimetric Methods for the Determination of the a-amino Nitrogen in Standard Solution and Fish Protein Hydrolysates." Journal of Food Research 1, no. 4 (2012): 174. http://dx.doi.org/10.5539/jfr.v1n4p174.

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<p>Two analytical methods, reverse phase high-performance liquid chromatography (RP-HPLC) and formol titration, were compared for their accuracy and precision for the determination of a-amino nitrogen (a-AN) concentration in fish protein hydrolysates. The result showed that, when tested with pure amino acid solutions, RP-HPLC method had a better standard deviation (S. D.), coefficient of variation (C. V.), and standard error (S. E.) but a poorer recovery rate compared with formol titration technique. The observed a-AN value by RP-HPLC has good coincidence with the actual a-AN value and t

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20

Bevers, Loes E., Peter-Leon Hagedoorn, Gerard C. Krijger, and Wilfred R. Hagen. "Tungsten Transport Protein A (WtpA) in Pyrococcus furiosus: the First Member of a New Class of Tungstate and Molybdate Transporters." Journal of Bacteriology 188, no. 18 (2006): 6498–505. http://dx.doi.org/10.1128/jb.00548-06.

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ABSTRACT A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized transport proteins ModA for molybdate and TupA for tungstate. Its structural gene is present in the genome of numerous archaea and some bacteria. The identification of this new tungstate and molybdate binding protein clarifies the mechanism of tungstate and molybdate transpor

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21

Frazier, Richard A., Athina Papadopoulou, Irene Mueller-Harvey, Dawn Kissoon, and Rebecca J. Green. "Probing Protein−Tannin Interactions by Isothermal Titration Microcalorimetry." Journal of Agricultural and Food Chemistry 51, no. 18 (2003): 5189–95. http://dx.doi.org/10.1021/jf021179v.

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22

Lawton, Daniel G., Christine Gorman, and Peter N. Lowe. "Small G protein characterisation by isothermal titration calorimetry." Biochemical Society Transactions 25, no. 3 (1997): 510S. http://dx.doi.org/10.1042/bst025510s.

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23

O'Donoghue, Geoff P., Alex A. Smoligovets, and Jay T. Groves. "Membrane Protein Cluster Titration in Early Lymphocyte Signaling." Biophysical Journal 102, no. 3 (2012): 32a. http://dx.doi.org/10.1016/j.bpj.2011.11.204.

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24

Liang, Yi. "Applications of isothermal titration calorimetry in protein science." Acta Biochimica et Biophysica Sinica 40, no. 7 (2008): 565–76. http://dx.doi.org/10.1111/j.1745-7270.2008.00437.x.

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25

Ullmann, G. Matthias, and Elisa Bombarda. "pKa values and redox potentials of proteins. What do they mean?" Biological Chemistry 394, no. 5 (2013): 611–19. http://dx.doi.org/10.1515/hsz-2012-0329.

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Abstract In this article, we review a microstate model that uses protonation and redox microstates in order to understand the complex pH and redox titration of proteins and other polyelectrolytes. From this model, it becomes obvious that it is impossible to assign pKa values or redox potentials to individual protonatable or redox-active sites in a protein in which many of such sites interact. Instead each site is associated with many microscopic equilibrium constants that may lead to irregular or even non-monotonic titration curves of some groups. The microstate model provides a closed theoret

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Simoes-Cardoso, Joao, Nanako Hoshino, Noriko Yoshimoto, and Shuichi Yamamoto. "Isothermal Titration Calorimetry as a Method for Analyzing Protein Adsorption in Ion Exchange Chromatography." MATEC Web of Conferences 333 (2021): 15001. http://dx.doi.org/10.1051/matecconf/202133315001.

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Ion exchange chromatography is a widely used method for purification of all types of biomolecules in current biotechnological downstream processes. Knowledge on the binding behavior of proteins provides valuable insight for understanding the molecular mechanisms of protein interactions in a biological context. However, thermodynamic parameters such as enthalpy and entropy changes that characterize protein adsorption are still unknown. Isothermal titration calorimetry applications in biosciences has gained its merit to study binding of soluble molecules, protein inhibition, conformational chang

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Simoes-Cardoso, Joao, Nanako Hoshino, Noriko Yoshimoto, and Shuichi Yamamoto. "Isothermal Titration Calorimetry as a Method for Analyzing Protein Adsorption in Ion Exchange Chromatography." MATEC Web of Conferences 333 (2021): 15001. http://dx.doi.org/10.1051/matecconf/202133315001.

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Ion exchange chromatography is a widely used method for purification of all types of biomolecules in current biotechnological downstream processes. Knowledge on the binding behavior of proteins provides valuable insight for understanding the molecular mechanisms of protein interactions in a biological context. However, thermodynamic parameters such as enthalpy and entropy changes that characterize protein adsorption are still unknown. Isothermal titration calorimetry applications in biosciences has gained its merit to study binding of soluble molecules, protein inhibition, conformational chang

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Cua, Simeon, Brenda A. Tello, Mafalda A. Farelo, et al. "A Novel Monoclonal Antibody Against a Modified Vaccinia Ankara (MVA) Envelope Protein as a Tool for MVA Virus Titration by Flow Cytometry." Viruses 16, no. 10 (2024): 1628. http://dx.doi.org/10.3390/v16101628.

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Modified vaccinia Ankara (MVA) virus is a widely used vaccine platform, making accurate titration essential for vaccination studies. However, the current plaque forming unit (PFU) assay, the standard for MVA titration, is prone to observer bias and other limitations that affect accuracy and precision. To address these challenges, we developed a new flow cytometry-based quantification method using a highly specific monoclonal antibody (mAb) for the detection of MVA-infected cells, as a more accurate titration assay. Through previous work, we serendipitously identified three MVA-specific hybrido

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Fernando, Luís Barroso da Silva, and MacKernan Donal. "Benchmarking a Fast Proton Titration Scheme in Implicit Solvent for Biomolecular Simulations." J. Chem. Theory Comput. 13, no. 6 (2017): 2915–29. https://doi.org/10.1021/acs.jctc.6b01114.

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pH is a key parameter for technological and biological processes, intimately related to biomolecular charge. As such, it controls biomolecular conformation and inter molecular interactions, for example, protein/RNA stability and folding, enzyme activity, regulation through conformational switches,protein-polyelectrolyte association, and protein-RNA interactions. pH also plays an important role in technological systems in food, brewing, pharma, bioseparations and biomaterials in general. Predicting the structure of large proteins and complexes remains a great challenge, experimentally, industri

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Wang, Yuanyuan, Zemao Gong, Han Fang, Dongming Zhi, and Hu Tao. "The N-terminal 1–55 residues domain of pyruvate dehydrogenase from Escherichia coli assembles as a dimer in solution." Protein Engineering, Design and Selection 32, no. 6 (2019): 271–76. http://dx.doi.org/10.1093/protein/gzz044.

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Abstract The pyruvate dehydrogenase complex (PDHc) from Escherichia coli is a large protein complex consisting of multiple copies of the pyruvate dehydrogenase (E1ec), dihydrolipoamide acetyltransferase (E2ec) and dihydrolipoamide dehydrogenase (E3ec). The N-terminal domain (NTD, residues 1–55) of E1ec plays a critical role in the interaction between E1ec and E2ec and the whole PDHc activity. Using circular dichroism, size-exclusion chromatography and dynamic light scattering spectroscopy, we show that the NTD of E1ec presents dimeric assembly under physiological condition. Pull-down and isoth

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Situ, Alan J., Thomas Schmidt, Parichita Mazumder, and Tobias S. Ulmer. "Characterization of Membrane Protein Interactions by Isothermal Titration Calorimetry." Journal of Molecular Biology 426, no. 21 (2014): 3670–80. http://dx.doi.org/10.1016/j.jmb.2014.08.020.

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Morel, Sandrine, Marc Chanson, Thien D. Nguyen, et al. "Titration of the gap junction protein Connexin43 reduces atherogenesis." Thrombosis and Haemostasis 112, no. 08 (2014): 390–401. http://dx.doi.org/10.1160/th13-09-0773.

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SummaryUbiquitous reduction of the gap junction protein Connexin43 (Cx43) in mice provides beneficial effects on progression and composition of atherosclerotic lesions. Cx43 is expressed in multiple atheroma-associated cells but its function in each cell type is not known. To examine specifically the role of Cx43 in immune cells, we have lethally irradiated low-density lipoprotein receptor-deficient mice and reconstituted with Cx43+/+, Cx43+/− or Cx43−/− haematopoietic fetal liver cells. Progression of atherosclerosis was significantly lower in aortic roots of Cx43+/− chimeras compared with Cx

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Guariento, Mara, Michael Assfalg, Serena Zanzoni, Dimitrios Fessas, Renato Longhi, and Henriette Molinari. "Chicken ileal bile-acid-binding protein: a promising target of investigation to understand binding co-operativity across the protein family." Biochemical Journal 425, no. 2 (2009): 413–24. http://dx.doi.org/10.1042/bj20091209.

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Protein–bile acid interactions are crucial microscopic events at the basis of both physiological and pathological biochemical pathways. BABPs (bile-acid-binding proteins) are intracellular transporters able to bind ligands with different stoichiometry, selectivity and co-operativity. The molecular determinants and energetics of interaction are the observables that connect the microscopic to the macroscopic frameworks. The present paper addresses the study and proposes a mechanism for the multi-site interaction of bile acids with chicken I-BABP (ileal BABP) with the aim of elucidating the deter

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Cin, Dilan, Pinar Soguksu, Meryem Merve Oren, Nuray Ozgulnar, Ali Agacfidan, and Sevim Mese. "The Anti-SARS-CoV-2 S-Protein IgG, Which Is Detected Using the Chemiluminescence Microparticle Immunoassay (CMIA) in Individuals Having Either a History of COVID-19 Vaccination and/or SARS-CoV-2 Infection, Showed a High-Titer Neutralizing Effect." Viruses 16, no. 9 (2024): 1409. http://dx.doi.org/10.3390/v16091409.

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Neutralizing antibodies plays a primary role in protective immunity by preventing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) from entering the cells. Therefore, characterization of antiviral immunity is important for protection against SARS-CoV-2. In this study, the neutralizing effect of the anti-SARS-CoV-2 S1 protein IgG, which was detected using the chemiluminescence microparticle immunoassay (CMIA)-based SARS-CoV-2 IgG II Quant (Abbott, Waukegan, IL, USA) test in SARS-CoV-2 infected and/or vaccinated individuals, was investigated with a surrogate virus neutralization test

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Ji, Ke, Kiheon Baek, Weicheng Peng, et al. "Biophysical and in silico characterization of NrtA: a protein-based host for aqueous nitrate and nitrite recognition." Chemical Communications 58, no. 7 (2022): 965–68. http://dx.doi.org/10.1039/d1cc05879g.

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36

Karonen, Maarit. "Polyphenol–Macromolecule Interactions by Isothermal Titration Calorimetry." Macromol 5, no. 1 (2025): 2. https://doi.org/10.3390/macromol5010002.

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Isothermal titration calorimetry (ITC) is a widely used and valuable technique for studying the binding interactions and the formation and dissociation of molecular complexes. ITC directly measures the energetics associated with the interactions and allows for a precise and complete thermodynamic description of association and binding processes, thereby providing an understanding of the interaction mechanisms. In this review, the role, practical aspects related to the experimental design and setup, advantages, and challenges of using ITC to evaluate polyphenol–macromolecule binding are discuss

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Prozeller, Domenik, Svenja Morsbach, and Katharina Landfester. "Isothermal titration calorimetry as a complementary method for investigating nanoparticle–protein interactions." Nanoscale 11, no. 41 (2019): 19265–73. http://dx.doi.org/10.1039/c9nr05790k.

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Isothermal titration calorimetry (ITC) is a complementary technique that can be used for investigations of protein adsorption on nanomaterials, as it quantifies the thermodynamic parameters of intermolecular interactionsin situ.

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SHEHADI, IHSAN A., ALEXEJ ABYZOV, ALPER UZUN, et al. "ACTIVE SITE PREDICTION FOR COMPARATIVE MODEL STRUCTURES WITH THEMATICS." Journal of Bioinformatics and Computational Biology 03, no. 01 (2005): 127–43. http://dx.doi.org/10.1142/s0219720005000916.

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THEMATICS (Theoretical Microscopic Titration Curves) is a simple, reliable computational predictor of the active sites of enzymes from structure. Our method, based on well-established Finite Difference Poisson–Boltzmann techniques, identifies the ionisable residues with anomalous predicted titration behavior. A cluster of two or more such perturbed residues is a very reliable predictor of the active site. The protein does not have to bear any resemblance in sequence or structure to any previously characterized protein, but the method does require the three-dimensional structure. We now present

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Dolezal, Olan, Larissa Doughty, Meghan K. Hattarki, et al. "Fragment Screening for the Modelling Community: SPR, ITC, and Crystallography." Australian Journal of Chemistry 66, no. 12 (2013): 1507. http://dx.doi.org/10.1071/ch13302.

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The SAMPL (Statistical Assessment of the Modelling of Proteins and Ligands) challenge brought together experimentalists and modellers in an effort to improve our understanding of chemical and biochemical systems so better modelling tools can be developed. The most recent challenge, SAMPL3, held at Stanford University in August 2011, was an attempt to improve the methods used to predict how small fragment compounds bind to proteins, and the protein chosen for this test was bovine trypsin. Surface plasmon resonance was used to screen 500 compounds from a Maybridge fragment library and these comp

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Amelia, Dara Callista Dinda, and Diah Astika Winahyu. "PENETAPAN KADAR PROTEIN JAMUR TIRAM PUTIH (Pleurotus ostreatus) DAN JAMUR MERANG (Volvariella volvaceae) DENGAN METODE KJELDAHL." Jurnal Analis Farmasi 6, no. 1 (2022): 90–94. http://dx.doi.org/10.33024/jaf.v6i2.5944.

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Mushrooms are plants that contain high levels of vitamin B complex and are the only non- animal foods with vitamin D content that are used for bone health.White oyster mushrooms and straw mushrooms have a lot of nutritional content, one of which is protein. Proteins are complex organic compounds of high molecular weight. Quantitative testing with the Kjedahl method has three stages, namely destruction, distillation, and titration. The results of the quantitative test using the Kjedahl method showed that oyster mushrooms had a protein content of 2.39% and straw mushrooms were 1.46%.

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Hammond, Michael S., R. Scott Houliston, and Elizabeth M. Meiering. "Two-dimensional 1H and 15N NMR titration studies of hisactophilin." Biochemistry and Cell Biology 76, no. 2-3 (1998): 294–301. http://dx.doi.org/10.1139/o98-032.

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We have used two-dimensional 1H-15N heteronuclear single quantum correlation spectroscopy to measure the pH dependence of backbone amide group chemical shifts in the actin binding protein hisactophilin over the pH range 5.7-11.1. Most of the resonances can be analyzed using a simple equation involving a single apparent ionization constant, pKapp. The majority of resonances in the protein titrate with pKapp values of 5.6-7.4. The results can be rationalized in terms of titration of many histidine residues in hisactophilin. The titration data provide direct experimental support for the proposed

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Lin, Iping G., Thomas J. Tomzynski, Qinglin Ou, and Chih-Lin Hsieh. "Modulation of DNA Binding Protein Affinity Directly Affects Target Site Demethylation." Molecular and Cellular Biology 20, no. 7 (2000): 2343–49. http://dx.doi.org/10.1128/mcb.20.7.2343-2349.2000.

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ABSTRACT It has recently been shown that in Xenopus, DNA demethylation at promoter regions may involve protein-DNA interactions, based on the specificity of the demethylated sites. Utilizing a stable episomal system in human cells, we recently mapped the sites and dissected the steps of demethylation at oriP sites bound by EBNA1 protein. Although it is clear that protein binding is required for demethylation of the oriP sites, it is uncertain whether this is a unique feature of the replication origin or whether it is a general phenomenon for all DNA sequences to which sequence-specific protein

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Witkowska, Danuta, Agnieszka Szebesczyk, Joanna Wątły, Michał Braczkowski, and Magdalena Rowińska-Żyrek. "A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains." International Journal of Molecular Sciences 22, no. 24 (2021): 13210. http://dx.doi.org/10.3390/ijms222413210.

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Combined potentiometric titration and isothermal titration calorimetry (ITC) methods were used to study the interactions of nickel(II) ions with the N-terminal fragments and histidine-rich fragments of Hpn-like protein from two Helicobacter pylori strains (11637 and 26695). The ITC measurements were performed at various temperatures and buffers in order to extract proton-independent reaction enthalpies of nickel binding to each of the studied protein fragments. We bring up the problem of ITC results of nickel binding to the Hpn-like protein being not always compatible with those from potentiom

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Boström, M., B. Lonetti, E. Fratini, P. Baglioni, and B. W. Ninham. "Why pH Titration in Protein Solutions Follows a Hofmeister Series." Journal of Physical Chemistry B 110, no. 14 (2006): 7563–66. http://dx.doi.org/10.1021/jp051025t.

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Teixeira, Andre Azevedo Reis, Mikael Lund, and Fernando Luís Barroso da Silva. "Fast Proton Titration Scheme for Multiscale Modeling of Protein Solutions." Journal of Chemical Theory and Computation 6, no. 10 (2010): 3259–66. http://dx.doi.org/10.1021/ct1003093.

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Leavitt, Stephanie, and Ernesto Freire. "Direct measurement of protein binding energetics by isothermal titration calorimetry." Current Opinion in Structural Biology 11, no. 5 (2001): 560–66. http://dx.doi.org/10.1016/s0959-440x(00)00248-7.

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Silverstein, Todd, Lüling Cheng, and John F. Allen. "Redox titration of multiple protein phosphorylations in pea chloroplast thylakoids." Biochimica et Biophysica Acta (BBA) - Bioenergetics 1183, no. 1 (1993): 215–20. http://dx.doi.org/10.1016/0005-2728(93)90022-8.

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IZUMI, T., K. HOSHIYAMA, S. ENOMOTO, K. SASAHARA, and Y. SUGIYAMA. "Determination of Protein Binding of Troglitazone Stereoisomers by Fluorometric Titration." Biopharmaceutics & Drug Disposition 18, no. 3 (1997): 241–57. http://dx.doi.org/10.1002/(sici)1099-081x(199704)18:3<241::aid-bdd17>3.0.co;2-o.

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Park, Jung Su, Nuree Kang, Yong Min Ahn, Yong Sik Kim, and Se Hyun Kim. "An International Adult Guideline for Making Clozapine Titration Safer by Using Ancestry-Based Personalized Dosing Titrations, C-Reactive Protein and Serum Clozapine Levels: Korean Translation and Its Clinical Application Cases." Korean Journal of Schizophrenia Research 26, no. 2 (2023): 52–60. http://dx.doi.org/10.16946/kjsr.2023.26.2.52.

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Objectives: During the initial clozapine titration, it is crucial to monitor for inflammatory reactions to ensure safe and effective administration. Clozapine metabolism varies by ancestry, particularly among Asians, warranting lower dosage. Recently, Dr. De Leon introduced guidelines based on ancestral differences. We aimed to provide a Korean translation, focusing on illustrating the necessity through clinical cases.Methods: The Korean translation of the guidelines, approved by Dr. De Leon, involved two psychiatrists who reviewed and revised each other’s work. An additional board-certified p

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Cramer, William A., Stanislav D. Zakharov, Genji Kurisu, and Yuko Misumi. "Isothermal Titration Calorimetric Analysis of Membrane Protein-Protein Interactions; Cytochrome b6f - Ferredoxin Nadp+ Reductase." Biophysical Journal 120, no. 3 (2021): 209a. http://dx.doi.org/10.1016/j.bpj.2020.11.1422.

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