Academic literature on the topic 'Rapd Analysis of P. Aeruginosa'

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Journal articles on the topic "Rapd Analysis of P. Aeruginosa"

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Auda, Ibtesam Ghadban, Israa M. S. Al-Kadmy, Sawsan Mohammed Kareem, et al. "RAPD- and ERIC-Based Typing of Clinical and Environmental Pseudomonas aeruginosa Isolates." Journal of AOAC INTERNATIONAL 100, no. 2 (2017): 532–36. http://dx.doi.org/10.5740/jaoacint.16-0267.

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Abstract Pseudomonas aeruginosa is a major cause of nosocomial infection in children and adults, resulting in significant morbidity and mortality due to its ability to acquire drug resistance. The ability of P. aeruginosa in the environment to cause infection in individuals has been reported previously; henceforth, surveillance of the emergence and transmission of P. aeruginosa strains among patients is important for infection control in a clinical setup. Various gene-typing methods have been used for epidemiological typing of P. aeruginosa isolates for the purpose of surveillance. In this wor
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Sallman, Ruqaia Sabbar, Suzan Saadi Hussein, and Munum Radwan Ali. "ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources." Al-Mustansiriyah Journal of Science 29, no. 2 (2018): 50. http://dx.doi.org/10.23851/mjs.v29i2.345.

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Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of a
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Speijer, Han, Paul H. M. Savelkoul, Marc J. Bonten, Ellen E. Stobberingh, and Jeroen H. T. Tjhie. "Application of Different Genotyping Methods forPseudomonas aeruginosa in a Setting of Endemicity in an Intensive Care Unit." Journal of Clinical Microbiology 37, no. 11 (1999): 3654–61. http://dx.doi.org/10.1128/jcm.37.11.3654-3661.1999.

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Colonization with Pseudomonas aeruginosa was studied by taking serial swab specimens from the oropharynges and anuses and tracheal and gastric aspirates from patients in an intensive care unit during a 10-month period in a setting of endemicity. Nineteen (10%) of the 192 patients included in the study were colonized on admission, while another 30 (16%) patients acquired P. aeruginosawhile in the hospital. Typing of 353 isolates was performed by random amplified polymorphic DNA (RAPD) analysis, and 56 strains were selected for further typing by RAPD analysis, pulsed-field gel electrophoresis (P
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Ravenni, N., P. Cocchi, S. Campana, C. Braggion, and G. Taccetti. "71 Follow-up of P. aeruginosa eradication in CF patients by RAPD analysis." Journal of Cystic Fibrosis 6 (June 2007): S17. http://dx.doi.org/10.1016/s1569-1993(07)60061-7.

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Li, Lingyan, Hongjiang Yang, Shuxiang Lin, and Shiru Jia. "Classification of 17 newly isolated virulent bacteriophages of Pseudomonas aeruginosa." Canadian Journal of Microbiology 56, no. 11 (2010): 925–33. http://dx.doi.org/10.1139/w10-075.

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Seventeen virulent bacteriophages specific to Pseudomonas aeruginosa strains were isolated by screening various environmental samples. These isolated bacteriophages were grouped based on results obtained from restriction fragment analysis of phage genomes, random amplification of polymorphic DNA (RAPD) typing, morphology observations under transmission electron microscope, and host range analysis. All 17 bacteriophages are double-stranded DNA viruses and can be divided into 5 groups based on DNA restriction profiles. A set of 10-mer primers was used in RAPD typing of phages, and similar conclu
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Heiba, Samy A. A., Ibthal S. El-Demerdash, and Shimaa E. Rashad. "Evaluation of Biological Control of Sorghum Strains Using Bacillus Thuringiensis and Pseudomonas Aeruginosa Under Drought Stress." Journal of Advanced Zoology 44, no. 4 (2023): 8–22. http://dx.doi.org/10.17762/jaz.v44i4.1321.

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Background: Sorghum is an economically significant staple food crop for more than half a billion people in developing nations, especially in arid and semi-arid locations where drought stress is a significant limiting factor. Despite usually being regarded as tolerant, sorghum suffers severely from drought stress, which lowers its productivity and nutritional quality throughout its principal cultivation areas. Objective: Improvements in DNA fingerprinting by ISSRs, SSRs, and RAPD markers have also been employed in sorghum genetic modification (GMOs) to enhance the economic characteristics of th
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Schauer, Bernhard, Regina Wald, Verena Urbantke, Igor Loncaric, and Martina Baumgartner. "Tracing Mastitis Pathogens—Epidemiological Investigations of a Pseudomonas aeruginosa Mastitis Outbreak in an Austrian Dairy Herd." Animals 11, no. 2 (2021): 279. http://dx.doi.org/10.3390/ani11020279.

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The present study describes an outbreak of Pseudomonas (P.) aeruginosa mastitis in a 20-cow dairy herd where throughout genotyping of isolates reusable udder towels were identified as the source of infection. Sampling of cows during three herd surveys and bacteriological culturing showed that P. aeruginosa was isolated from nine cows with a total of 13 infected quarters. Mastitis occurred as mild clinical or subclinical infection. P. aeruginosa was additionally isolated from a teat disinfectant solution, containing N-(3-aminopropyl)-N-dodécylpropane-1,3-diamine 1 as active component, and micro
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Verónica, Jocelyne Flores-Velázquez, and Pérez-y.-Terrón Rocío. "Pseudomonas aeruginosa: Mechanisms of resistance to antibiotics and case analysis." GSC Biological and Pharmaceutical Sciences 14, no. 3 (2021): 179–88. https://doi.org/10.5281/zenodo.4657017.

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<em>Pseudomonas aeruginosa</em>&nbsp;is an opportunistic pathogen, causing great concern due to the rapid increase in its resistance to antibiotics. The objective of the research was to describe the resistance mechanisms that&nbsp;<em>P. aeruginosa</em>&nbsp;possesses, as well as to report its behavior against antibiotics in the years 2003 to 2018 in Mexico. A retrospective and longitudinal documentary research was carried out in different digital resources referring to antibiotic resistance in&nbsp;<em>P. aeruginosa</em>. The results showed that the main resistance mechanisms of&nbsp;<em>P. a
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Verónica Jocelyne Flores-Velázquez and Rocío Pérez-y-Terrón. "Pseudomonas aeruginosa: Mechanisms of resistance to antibiotics and case analysis." GSC Biological and Pharmaceutical Sciences 14, no. 3 (2021): 179–88. http://dx.doi.org/10.30574/gscbps.2021.14.3.0066.

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Pseudomonas aeruginosa is an opportunistic pathogen, causing great concern due to the rapid increase in its resistance to antibiotics. The objective of the research was to describe the resistance mechanisms that P. aeruginosa possesses, as well as to report its behavior against antibiotics in the years 2003 to 2018 in Mexico. A retrospective and longitudinal documentary research was carried out in different digital resources referring to antibiotic resistance in P. aeruginosa. The results showed that the main resistance mechanisms of P. aeruginosa are β-lactamases, ejection pumps, mutations in
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Quintero-Garrido, Karen Guadalupe, Fátima Berenice Ramírez-Montiel, Marilú Chávez-Castillo, et al. "Antibacterial behavior and bacterial resistance analysis of P. aeruginosa in contact with copper nanoparticles." Mexican journal of biotechnology 8, no. 1 (2023): 1–20. http://dx.doi.org/10.29267/mxjb.2023.8.1.1.

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The present study describes the antibacterial behavior and the bacterial resistance analysis of extremophile Pseudomonas aeruginosa in contact with copper nanoparticles (CuNPs). For this purpose, green synthesis of CuNPs was performed by combined ultrasound-assisted and chemical reduction methods, obtaining semispherical CuNPs ranging from ca. 4-9 nm. Antibacterial activity (AA) of biosynthesized CuNPs demonstrates an antibacterial inhibition of 85 % (LD85) at 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL after 3 h of contact. Bacterial adaptation in contact with CuNPs
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Dissertations / Theses on the topic "Rapd Analysis of P. Aeruginosa"

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Cambronel, Melyssa. "Analyse de la réponse physiologique et génétique de Pseudomonas aeruginosa et Enterococcus faecalis envers l'épinéphine et recherche de senseurs putatifs. Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103 Influence of catecholamines (epinephrine/norepinephrine) on biofilm formation and adhesion in pathogenic and probiotic strains of Enterococcus faecalis Evaluation of probiotic properties and safety of Enterococcus faecium isolated from artisanal Tunisian meat "dried ossban" Probiotic potential and safety evaluation of Enterococcus faecalis OB14 and OB15, isolated from traditional Tunisian Testouri cheese and Rigouta, using physiological and genomic analysis Draft genome sequence of Enterococcus faecalis strain OB15, a probiotic strain recently isolated from "Tunisian Rigouta Cheese"." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR106.

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L’endocrinologie microbienne a pour objet d’étude les interactions qui peuvent survenir entre les bactéries et les molécules sécrétées par l’hôte. Pseudomonas aeruginosa et Enterococcus faecalis sont deux pathogènes opportunistes impliqués dans des infections nosocomiales. Leur présence au sein du corps humain, les place donc au contact de molécules eucaryotes, dont les catécholamines (épinéphrine et norépinéphrine) qui sont produites en cas de réponse « combat-fuite » mais aussi lors de trauma, ou d’actes chirurgicaux. Ces substances sont connues pour être capables de moduler la physiologie b
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Lee, Huey Jen, and 李慧真. "Analysis of Genetic Variation of Pseudocercospora fuligena and P. atromarginalis by Random Amplified polymorphic DNA (RAPD) technique." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/31131496415158966156.

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碩士<br>國立中興大學<br>植物病理學系<br>84<br>The fungi tested in this study included each 33 isolates of Pseudocercosporafuligena (on Lycopersicon esculentum ) and P. atromarginalis (on Solanumnigrum), and 20 other isolates of Cercospora spp. and Pseudocercospora spp. wereused to perform analysis of their genetic variation by RAPD. Based on thepreliminary tests of searching suitable RAPD conditions, the results revealedthat optimal reaction mixtures contained DNA ( 50 ng ), 1 x buffe
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Book chapters on the topic "Rapd Analysis of P. Aeruginosa"

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Kumar, Sunil, and Sonal Malhotra. "The Role of Pseudomonas aeruginosa DsbA-1 in Bacterial Pathogenesis: Current Research and Future Prospects." In Pseudomonas aeruginosa - New Perspectives and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.108072.

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Disulfide bond isomerase proteins (Dsbs) have been extensively characterized in gram-negative bacteria. Recently research efforts is being placed on their biology in gram-positive species. Modern “omics” technologies, allowed assessment of the contribution of the Dsbs to bacterial pathogenesis. The author cloned and characterized the dsbA 1 protein from Pseudomonas aeruginosa in the late 1990s. The global proteome analysis demonstrated that the dsbA gene is under the direct regulatory control of the extracytoplasmic function (ECF) sigma factor AlgT(U) or sigma-22. This is unique to P. aerugino
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de Sousa, Danyelle Gomes, Yasmin Macedo de Sousa, Kelry de Souza Fechine Andrade, et al. "GC-MS ANALYSIS AND IN VITRO ANTIMICROBIAL ACTIVITY OF Ocotea catharinesis MEZ essential oil (LAURACEAE)." In Knowledge Integration: A Multidisciplinary Approach to Science. Seven Editora, 2025. https://doi.org/10.56238/sevened2025.018-046.

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The use of plants to treat and prevent diseases is an ancestral practice of humanity. In recent years, the resistance of microorganisms to antibiotics has generated a growing interest in the research of therapeutic alternatives, including the chemical compounds present in essential oils. The aim of the present study was to evaluate the antibacterial and antifungal effect of Ocotea catharinesis essential oil (EOOc) and to identify its main compounds. The extraction of the essential oil was done by hydrodistillation for 4.5 hours, using 50g of dried leaves. To identify the chemical components, g
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Conference papers on the topic "Rapd Analysis of P. Aeruginosa"

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Zhou, Enze, Huabing Li, Dake Xu, and Jianjun Wang. "Accelerated Corrosion of 2304 Duplex Stainless Steel by Marine Pseudomonas Aeruginosa Biofilm." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09326.

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Abstract Microbiologically Influenced Corrosion (MIC) in the marine environment has caused a serious threat to the safety of marine materials. The corrosion rate of MIC is usually much faster than the general corrosion process. The 2304 duplex stainless steel (DSS) is an excellent alternative to 316L SS in marine applications, while its MIC behavior is barely known. In this work, surface analysis and electrochemical techniques were used to study the corrosion behavior of 2304 DSS caused by the marine aerobe Pseudomonas aeruginosa. Compared with the abiotic control, the largest pit depth showed
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Xu, Dake, Tong Xi, Jin Xia Liaoning, Chunguang Yang, Qi Li, and Ke Yang. "The Microbiologically Influenced Corrosion (MIC) Resistance Behavior of 2205 Cu-bearing Duplex Stainless Steel in the Presence of Aerobic Marine Pseudomonas Aeruginosa Biofilm." In CORROSION 2016. NACE International, 2016. https://doi.org/10.5006/c2016-07357.

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Abstract Recently, more and more reports indicated that microbiologically influenced corrosion (MIC) severely influences the safety of petroleum installations and marine infrastructures. Conventional MIC mitigation methods are physical scrubbing, coatings, and biological or biocide treatments to inhibit the corrosive biofilm. A novel antibacterial 2205-Cu duplex stainless steel (DSS) was developed from the material aspect to mitigate the corrosive marine biofilm by the release of Cu2+ and copper-rich phases on the antibacterial DSS surface. In this study, the MIC resistance behavior of a 2205
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Ifah, Arini Al, Endang Yuniastuti, and Parjanto. "Analysis of breadfruit plant diversity (Artocarpus altilis P.) by random amplified polymorphic DNA (RAPD) in DIY." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062802.

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Johnson, Brian, Robert Badal, Samuel K. Bouchillon, et al. "Trends In Susceptibility Of P. Aeruginosa To Piperacillin-Tazobactam And Comparators 2004-2009 - Global T.E.S.T. Analysis." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a3353.

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Rousseau, S., C. Su, G. Martel, P. Bortolotti, and A. Emad. "A Network-guided Analysis of P. Aeruginosa-infected Bronchial Epithelial Cells Reveals Putative Regulators of Viral-mediated Pulmonary Exacerbations." In American Thoracic Society 2023 International Conference, May 19-24, 2023 - Washington, DC. American Thoracic Society, 2023. http://dx.doi.org/10.1164/ajrccm-conference.2023.207.1_meetingabstracts.a5671.

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Branković, Jovica, Zorica D. Petrović, and Vladimir P. Petrović. "In silico antibiofilm potency of phenolic N-acyl hydrazones against selected bacterial strains." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.495b.

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In the present work, fourteen phenolic hydrazone derivatives were evaluated for their in silico inhibitory activity against selected P. aeruginosa and S. maltophilia proteins involved in drug resistance and biofilm formation. Molecular docking analysis revealed the highest binding affinity of analogs n (-8.4 kcal/mol) and h (-7.3 kcal/mol) towards P. aeruginosa 7m1m and 7m1l proteins, respectively. In the case of S. maltophilia, analog k (-8.4 kcal/mol) expressed the highest binding affinity to 6qw7, whereas for 6uaf, the lowest binding energy was calculated for derivative d (-8.1 kcal/mol). T
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Karadžić, Vesna, Jelena Knežević, and Dragana Jovanović. "Freshwater cyanobacteria: A threat to ecosystem stability and public health." In Proceedings of the International Congress Public Health - Achievements and Challenges. Institute of Public Health of Serbia "Dr Milan Jovanović Batut", 2024. http://dx.doi.org/10.5937/batutphco24018k.

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Background: The abundant growth of cyanobacteria due to increased anthropogenic eutrophication and global climate change has created serious concerns about harmful bloom formation and contamination of surface waters worldwide, especially water sources used for drinking water supply. Cyanobacterial blooms followed by producing toxic compounds represent serious ecological consequences with a high risk for ecosystem stability and global public health. The absence or rare monitoring of freshwater cyanobacteria's presence (and abundance) and the lack of health risk assessment increases the threat t
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Reports on the topic "Rapd Analysis of P. Aeruginosa"

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ทัศนาขจร, อัญชลี. การศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ โดยการตรวจลายพิมพ์ดีเอ็นเอ ด้วยตัวตรวจสอบที่มีลำดับเบสซ้ำแบบง่าย : รายงานวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์์มหาวิทยาลัย, 1997. https://doi.org/10.58837/chula.res.1997.19.

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ในการศึกษาความแตกต่างแปรผันทางพันธุกรรมในประชากรกุ้งกุลาดำ ได้ทดสอบวิธีการตรวจลายพิมพ์ดีเอ็นเอในกุ้งกุลาดำและได้เลือก 2 วิธี เพื่อใช้ในการศึกษาความแตกต่างแปรผันทางพันธุกรรม คือ 1. การตรวจลายพิมพ์ดีเอ็นเอ โดยเทคนิค RAPD (Random Amplified Polymorphic DNA analysis) และ 2. การตรวจความแปรผันของ microsatellites DNA ในการศึกษานี้ได้เก็บตัวอย่างกุ้งจากแหล่งที่นิยมใช้เป็นพ่อ-แม่พันธุ์ในการเพาะเลี้ยงกุ้ง จำนวน 5 แหล่ง โดยเก็บตัวอย่างจากทะเลอันดามัน 3 แหล่ง ได้แก่ กุ้งบริเวณ จ. สตูล-ตรัง กุ้งจากจ. พังงา และกุ้งจากเมดาน ประเทศอินโดนีเซีย และเก็บตัวอย่างกุ้งจากฝั่งอ่าวไทย 2 แหล่ง ได้แก่ กุ้งจาก จ. ตราด และ
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พงษ์สามารถ, สุนันท์, วิมลมาศ ลิปิพันธ์ та พนิดา วยัมหสุวรรณ. ศึกษาสารสกัดโพลีแซคคาไรด์จากเปลือกของผลทุเรียนเพื่อใช้ประโยชน์ทางการแพทย์. จุฬาลงกรณ์มหาวิทยาลัย, 2003. https://doi.org/10.58837/chula.res.2003.28.

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แยกสารโพลีแซคคาไรด์ได้ 2 ชนิด จากเปลือกแห้งของผลทุเรียน (Durio zibethinus L.) เตรียมในรูปผงแห้งของ polysaccharide gel (PG) และ polysaccharide fiber (PF) องค์ประกอบธาตุหลักของโพลีแซคคาไรด์ประกอบด้วย Carbon (C) Hydrogen (H) และ Oxygen (O) ในสัดส่วนอะตอมของ C:H:O เท่ากับ 2.9:5.3:3.1 และ 3.5:6.4:3.1 ใน PG และ PF ตามลำดับ PG มีองค์ประกอบของ acidic sugar ได้แก่ galacturonic acid มากกว่าห้าสิบเปอร์เซนต์ ของน้ำหนัก PG และมีน้ำตาล neutral sugar ได้แก่ glucose fructose rhamnose และ arabinose ใน PF มีองค์ประกอบของน้ำตาล glucose เพียงชนิดเดียว ทำการตรวจวิเคราะห์น้ำตาลโดยวิธี colorimetric method TLC และ HP
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Droby, Samir, Joseph W. Eckert, Shulamit Manulis, and Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568777.bard.

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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-Aspire
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