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Journal articles on the topic 'Rapd Analysis of P'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Timme, Stephen L., and Alan Ross. "A simple method to extract DNA from bryophyes for RAPD analysis." Evansia 14, no. 4 (1997): 131–32. http://dx.doi.org/10.5962/p.346420.

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2

Nkongolo, K. K., P. Michael, and T. Demers. "Application of ISSR, RAPD, and cytological markers to the certification of Picea mariana, P. glauca, and P. engelmannii trees, and their putative hybrids." Genome 48, no. 2 (2005): 302–11. http://dx.doi.org/10.1139/g04-118.

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Picea glauca (white spruce) and P. engelmannii (Engelmann spruce) are so similar and integrated that it is impossible to distinguish between them and their hybrids using morphological characteristics. Although natural hybrids between P. glauca and P. mariana (black spruce) do not generally occur, even though the 2 species are sympatric in North America, a first-generation hybrid, called the Rosendahl spruce, has been reported in the literature. In this study, several inter-simple sequence repeat (ISSR) markers were developed, as were randomly amplified polymorphic DNA (RAPD) markers, to certif

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Bakonyi, J., and A. F. Justesen. "Genetic Relationship ofPyrenophora graminea,P. teresf.maculataandP. teresf.teresAssessed by RAPD Analysis." Journal of Phytopathology 155, no. 2 (2007): 76–83. http://dx.doi.org/10.1111/j.1439-0434.2007.01192.x.

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Evren, Özay Hasan, Ertuǧrul Yüzbaşıoǧlu, and Mehmet Yaşar Dadandı. "Determination of intra-specific genetic variation of Phlomis kurdica and Phlomis oppositiflora and investigation for the hybridity of P. x melitenense (Lamiaceae) by means of molecular markers." Biologia 70, no. 9 (2015): 1159–71. http://dx.doi.org/10.1515/biolog-2015-0132.

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Abstract In this study, intra-specific genetic variation and inter-specific genetic relation were investigated among Phlomis oppositiflora, P. kurdica, P. × melitenense (P. kurdica × oppositiflora), P. capitata and P. kurdica × capitata by using RAPD and ISSR markers. The hybridity of P. × melitenense and P. kurdica × capitata samples was also surveyed in terms of morphological and molecular aspects. Except for two, all bands obtained from RAPD (707 bands) and ISSR (651 bands) analyses were polymorphic. The lowest genetic distance values obtained from RAPD and ISSR analyses were 0.0156 (betwee

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Wu, J., K. V. Krutovskii, and S. H. Strauss. "Nuclear DNA diversity, population differentiation, and phylogenetic relationships in the California closed-cone pines based on RAPD and allozyme markers." Genome 42, no. 5 (1999): 893–908. http://dx.doi.org/10.1139/g98-171.

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We studied nuclear gene diversity and population differentiation using 91-98 randomly amplified polymorphic DNA (RAPD) loci in the California closed-cone pines knobcone (Pinus attenuata Lemm.), bishop (P. muricata D. Don), and Monterey (P. radiata D. Don) pines. A total of 384 trees from 13 populations were analyzed for RAPDs and another sample of 242 trees from 12 of these 13 populations were analyzed at 32-36 allozyme loci, using a published data set. Twenty-eight of 30 (93%) comigrating RAPD fragments tested were found to be homologous by Southern hybridization in all three species. Using a

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Bayazit, S., B. Imrak, A. Küden, and M. Kemal Güngör. "RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey." Horticultural Science 38, No. 4 (2011): 134–41. http://dx.doi.org/10.17221/97/2011-hortsci.

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Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10,

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Clerc, Agathe, Charles Manceau, and Xavier Nesme. "Comparison of Randomly Amplified Polymorphic DNA with Amplified Fragment Length Polymorphism To Assess Genetic Diversity and Genetic Relatedness within Genospecies III ofPseudomonas syringae." Applied and Environmental Microbiology 64, no. 4 (1998): 1180–87. http://dx.doi.org/10.1128/aem.64.4.1180-1187.1998.

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ABSTRACT Recently, DNA pairing analyses showed that Pseudomonas syringae pv. tomato and related pathovars, includingP. syringae pv. maculicola, form a genomic species (Pseudomonas tomato) (L. Gardan, H. L. Shafik, and P. A. D. Grimont, p. 445–448, in K. Rudolph, T. J. Burr, J. W. Mansfield, D. Stead, A. Vivian, and J. von Kietzell, ed., Pseudomonas syringae Pathovars and Related Pathogens, 1997). The genetic diversity of 23 strains belonging to this genomic species and 4 outgroup strains was analyzed with randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphic (AFLP

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8

Ozbey, G., A. Kilic, H. B Ertas, and A. Muz. "Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats." Veterinární Medicína 49, No. 3 (2012): 65–69. http://dx.doi.org/10.17221/5678-vetmed.

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n this study, 30, 15 and 1 strains of &lt;i&gt;Pasteurella multocida&lt;/i&gt; and 9, 8 and 6 strains of &lt;i&gt;Mannheimia haemolytica&lt;/i&gt; from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of &lt;i&gt;P. multocida&lt;/i&gt; isolates from cattle and sheep, respectively. However, &lt;i&gt;M. haemolytica&l

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Mobasherizadeh, Sina, Hasan Shojaei, Seyed Asghar Havaei, et al. "Application of the Random Amplified Polymorphic DNA (RAPD) Fingerprinting to Analyze Genetic Variation in Community Associated-Methicillin Resistant Staphylococcus Aureus (CA-MRSA) Isolates in Iran." Global Journal of Health Science 8, no. 8 (2015): 185. http://dx.doi.org/10.5539/gjhs.v8n8p185.

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<p>The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.</p><p>The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The amplified PCR products were detected on a 1.5% agarose gel and subjected to further analysis to establish the band profiles and genetic relationships using the Gel Compar® prog

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Ganter, Philip F., and Bryan Quarles. "Analysis of population structure of cactophilic yeast from the genusPichia:P.cactophilaandP.norvegensis." Canadian Journal of Microbiology 43, no. 1 (1997): 35–44. http://dx.doi.org/10.1139/m97-006.

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DNAs from 40 strains of Pichia cactophila and Pichia norvegensis, yeasts characteristic of cactus necroses, were compared using randomly amplified polymorphic DNA (RAPD) banding patterns and killer/sensitive phenotypes. Both species belong to the same species complex within the genus. The levels of between-strain RAPD variation were high in both species (higher in the automictic P. cactophila than in the heterothallic P. norvegensis), although there is little variation in physiological abilities within either species. Although each species was a separate lineage, RAPD analysis confirms that th

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Hadiati, Sri, Riry Prihatini, and Ellina Mansyah. "Identifikasi Molekuler dan Analisis Kekerabatan Aksesi Nenas Menggunakan Marka RAPD Menunjang Perakitan Varietas Unggul Baru." Jurnal Hortikultura 28, no. 1 (2019): 1. http://dx.doi.org/10.21082/jhort.v28n1.2018.p1-12.

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<p><strong>(</strong><em><strong>Molecular Identification and Relationships Among Several Pineapple Accessions Using RAPD Marker to Support the Assembling New Varieties</strong></em><strong>)</strong></p><p>Produksi dan produktivitas nenas dapat ditingkatkan antara lain melalui penggunaan varietas unggul. Dalam perakitan varietas, dibutuhkan informasi hubungan kekerabatan antartetuanya agar diperoleh efek heterosis yang tinggi melalui kegiatan identifikasi secara molekuler. Penelitian bertujuan (1) mengetahui tingkat polimorfism

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Kafkas, Salih, and Rafael Perl-Treves. "Interspecific Relationships in Pistacia Based on RAPD Fingerprinting." HortScience 37, no. 1 (2002): 168–71. http://dx.doi.org/10.21273/hortsci.37.1.168.

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Phylogenetic relationships among nine species in the genus Pistacia were studied by randomly amplified polymorphic DNA (RAPD) analysis. The following species were included: P. atlantica, P. terebinthus, P. eurycarpa, P. vera, P. integerrima, P. mexicana, P. palaestina, P. lentiscus, and P. khinjuk. Genomic DNA was extracted from leaf tissue and RAPD analysis was performed using 20 primers. A total of 242 fragments were generated and 228 bands were polymorphic at the inter-specific level. Subjecting these data to phylogenetic analysis yielded a shortest cladogram that is 338 steps long, featuri

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Gaafar, Reda, Mai Allam, Rasha Sabry, and Mahmoud Saker. "Molecular genetic analysis of some North African barley germplasms." Acta agriculturae Slovenica 109, no. 2 (2017): 187. http://dx.doi.org/10.14720/aas.2017.109.2.03.

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<p>Isozyme and RAPD markers were used to characterize 29 barley accessions, which were collected from North Africa. In addition, resistance gene sequences were employed to develop molecular markers using RT-PCR approach. High level of polymorphism was found with both RAPD and isozyme markers, where RAPD showed that 60 % of amplified bands were polymorphic. Peroxidase showed three polymorphic loci (7 allelic bands). Isozymes cluster analysis successfully separated the barley accessions into three geographically distinct groups. RAPD investigation demonstrated that Egyptian accessions were

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SAIDI, Abbas, and Melika HAJKAZEMIAN. "Comparative assessment of ISSR, DAMD and RAPD markers for evaluation of genetic diversity of gerbera (Gerbera jamesonii Bolus ex Hooker f.) cultivars." Acta agriculturae Slovenica 119, no. 1 (2023): 1. http://dx.doi.org/10.14720/aas.2023.119.1.2425.

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<p class="042abstractstekst"><span lang="EN-US">Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The

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Nosrati, Houshang, and Hanife Akbarian. "Impact of habitat fragmentation on genetic structure of Capparis spinosa populations revealed by ISSR markers." Environmental and Experimental Biology 20, no. 4 (2023): 219–24. http://dx.doi.org/10.22364/eeb.20.20.

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The results from empirical studies on the impact of habitat fragmentation on genetic variation of populations are controversial, ranging from negative to positive effects. This contradiction has been attributed to either species characteristics (e.g. plant life history) or environmental components (e.g. the scale of habitat fragmentation). To examine the role of DNA marker types on these effects, we carried out a comparative study of seven populations of Capparis spinosa L. (caper, Capparaceae) using ISSR markers and compared these results with those obtained previously using RAPD markers. Alt

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Almeida, Francisca C., Miguel A. M. Moreira, Cibele R. Bonvicino, and Rui Cerqueira. "RAPD analysis of Nectomys squamipes (Rodentia, Sigmodontinae) populations." Genetics and Molecular Biology 23, no. 4 (2000): 793–97. http://dx.doi.org/10.1590/s1415-47572000000400014.

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Random amplified of polymorphic DNA (RAPD) analysis was used to assess genetic distance and the genetic structure of populations of Nectomys squamipes, a semiaquatic rodent species distributed along watercourses. DNA samples of five populations were analyzed using three primers, producing 45 scorable bands, 31 of which were polymorphic. There was a significant differentiation among populations [F ST = 0.17; phiST = 0.14 (P < 0.004)] but gene flow (Nm = 1.25) was sufficient to overcome genetic drift effects. No fixed specific markers were found for any population. The Mantel's test and UPGMA

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Moschetti, Giancarlo, Giuseppe Blaiotta, Francesco Villani, Salvatore Coppola, and Eugenio Parente. "Comparison of Statistical Methods for Identification ofStreptococcus thermophilus,Enterococcus faecalis, and Enterococcus faecium from Randomly Amplified Polymorphic DNA Patterns." Applied and Environmental Microbiology 67, no. 5 (2001): 2156–66. http://dx.doi.org/10.1128/aem.67.5.2156-2166.2001.

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ABSTRACT Thermophilic streptococci play an important role in the manufacture of many European cheeses, and a rapid and reliable method for their identification is needed. Randomly amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) with two different primers coupled to hierarchical cluster analysis has proven to be a powerful tool for the classification and typing of Streptococcus thermophilus,Enterococcus faecium, and Enterococcus faecalis (G. Moschetti, G. Blaiotta, M. Aponte, P. Catzeddu, F. Villani, P. Deiana, and S. Coppola, J. Appl. Microbiol. 85:25–36, 1998). In order to develop a fast and

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Ravenni, N., P. Cocchi, S. Campana, C. Braggion, and G. Taccetti. "71 Follow-up of P. aeruginosa eradication in CF patients by RAPD analysis." Journal of Cystic Fibrosis 6 (June 2007): S17. http://dx.doi.org/10.1016/s1569-1993(07)60061-7.

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ZABET, Mohammad, Leyli ESMAEILZAEI, Ali IZANLOO, and Seyyed Hamid Reza RAMAZANI. "Genetic variation and cluster analysis of Narcissus tazetta L. genotypes using RAPD and ISSR markers." Acta agriculturae Slovenica 119, no. 1 (2023): 1. http://dx.doi.org/10.14720/aas.2023.119.1.2716.

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<p class="042abstractstekst">To evaluate the genetic diversity of Iranian <em>Narcissus</em> genotypes (e.g., Shomal, Shastpar, Shahla, Yasuj, Shiraz-1, Shiraz-2, Kuchak-e-Atri, Dutch, Khosf-1, Khosf-2, Birjand, and Tabas), different RAPD and ISSR primers were examined at the Plant Breeding Laboratory of the Faculty of Agriculture, University of Birjand, Iran. In sum, RAPD and ISSR primers produced 189 and 80 high-resolution bands. The average values of PIC, Ht, Hs, Nm, DST, FDT, NA, Ne, H, and I indices were 0.287, 0.369, 0.089, 0.486, 0.279, 0.760, 1.952, 1.459, 0.282, 0.43

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Staub, Jack E., Gennaro Fazio, Thomas Horejsi, Yael Danin-Poleg, Noa Reis, and Nurit Katzir. "050 Comparative Analysis of Cultivated Melon Groups (Cucumis melo L.) using Random Amplified Polymorphic DNA and Simple Sequence Repeat Markers." HortScience 35, no. 3 (2000): 397B—397. http://dx.doi.org/10.21273/hortsci.35.3.397b.

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Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to characterize genetic relationships among 46 accessions in two C. melo L. subsp. melo (Cantalupensis, Inodorus) and subsp. agrestis (Conomon and Flexuosus) groups. Genetic distance (GD) estimates were made among and between accessions in four melon market classes [Galia, Ogen, Charentais, and Shipper (European and U.S. types)] of Cantalupensis, one market class of Inodorus (Cassaba and Honey Dew), one accession of Conomon, and one accession of Flexuosus by employing three GD estimators; simple matching

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Dariush, S., A. A. Ebadi, M. Khoshkdaman, B. Rabiei, and A. Elahinia. "Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran." Plant Protection Science 48, No. 4 (2012): 162–69. http://dx.doi.org/10.17221/64/2011-pps.

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Sheath rot of rice and leaf blight of wheat caused by Pseudomonas syringae pv. syringae are the important bacterial pathogens of rice and wheat in Iran. The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity of 60 strains of P. s. pv. syringae obtained from rice and wheat in different growth stages. Cluster analysis by UPGMA method showed that strains were grouped into two clusters. The AMOVA analysis indicated that about 18% of the total genetic variation existed between two populations of rice and wheat, which showed the lack of host specialization

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Park, S. O., A. Dursun, D. P. Coyne, and G. Jung. "Identification of RAPD Markers Linked to Major Genes for Common Bacterial Blight (CBB) Resistance Using Bulked Segregant Analysis in a Tepary Bean Cross." HortScience 31, no. 4 (1996): 623d—623. http://dx.doi.org/10.21273/hortsci.31.4.623d.

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Common bacterial blight (CBB), incited by Xanthomonas campestris pv. phaseoli (Xcp), an important disease in common bean (Phaseolus vulgaris L.) Tepary bean (P. acutifolius A. Gray) is of interest to bean breeders because of resistance to CBB. Our objective was to identify RAPD markers linked to major genes for CBB resistance using bulked segregant analysis in an F2 population from a tepary bean cross CIAT640005 (R) X Nebr#4B (S). A total of 57 RAPD primers (602 RAPD primers screened) showed polymorphisms between bulked DNA derived from R and S CBB plants. All markers showed coupling linkage w

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Wei, Jun-Zhi, and Richard R. C. Wang. "Genome- and species-specific markers and genome relationships of diploid perennial species in Triticeae based on RAPD analyses." Genome 38, no. 6 (1995): 1230–36. http://dx.doi.org/10.1139/g95-161.

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Eight different genomes (E, H, I, P, R, St, W, and Ns) represented by 22 diploid species of the tribe Triticeae were analyzed using the random amplified polymorphic DNA (RAPD) technique. The genome relationships were obtained based on 371 RAPD fragments produced with 30 primers. The four species of the genus Psathyrostachys (having various Ns genomes) were closely related. The genomes Ee and Eb had a similarly close relationship and were distinct from all other genomes analyzed. Genomes P, R, and St were grouped in one cluster and genomes H and I in another. Genome W had a distant relationship

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Desem, Muhammad Ibrahim, Ekowati Handharyani, Agus Setiyono, Safika Safika, Didik Tulus Subekti, and Fitrine Ekawasti. "Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia." Veterinary Medicine International 2023 (March 11, 2023): 1–9. http://dx.doi.org/10.1155/2023/7778707.

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Pasteurella multocida is a Gram-negative bacterium that causes hemorrhagic septicemia (HS) in buffaloes and cattle. The disease causes serious problems in Indonesian livestock and is classified as a serious transmissible animal disease. Previous research has determined the diversity of P. multocida using a serotyping method based on the antigenic properties of capsule polysaccharides. An alternative method for analysis utilizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD). This study aimed to characterize and determine P. mult

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Sianipar, Nesti Fronika, Reflinur, Muhammad Dzulkifly Ashan, Khoirunnisa Assidqi, and Ragapadmi Purnamaningsih. "Identification of Specific DNA Fragments in the Superior Mutant Plant of Rodent Tuber Accession Pekalongan (<i>Typhonium flagelliforme</i>) Based on Sequencing Analysis." Materials Science Forum 1069 (August 31, 2022): 231–38. http://dx.doi.org/10.4028/p-78ru0f.

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Cancer is a large group of diseases that occur in almost any organ or tissue of the body when abnormal cells get out of normal and invade adjacent cells. In women especially, breast cancer is among the most common types of cancer. These cancer cells will form a tumor that can be seen on an x-ray. Some traditional breast cancer treatments have been carried out, including using local herbal plants such as rodent tubers (Typhonium flagelliforme). Rodent tuber contains stigmasterol which has a high anticancer effect and was found in several mutant clones. The purpose of this study was to analyze D

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de Wet, Juanita, Michael J. Wingfield, Teresa A. Coutinho, and Brenda D. Wingfield. "Characterization of Sphaeropsis sapinea Isolates from South Africa, Mexico, and Indonesia." Plant Disease 84, no. 2 (2000): 151–56. http://dx.doi.org/10.1094/pdis.2000.84.2.151.

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Post-hail-associated dieback of Pinus patula and P. radiata trees, induced by Sphaeropsis sapinea (=Diplodia pinea), is a common and important disease in commercial pine plantations. Two morphotypes, A and B, have been described for this fungus based on differences in cultural characteristics, conidial morphology, and virulence among isolates from the north central United States. The existence of the two described morphotypes was later verified through the use of random amplified polymorphic DNA (RAPD) analyses, and the morphotypes were designated as the A and B RAPD marker groups. The objecti

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Teng, Yuanwen, Kenji Tanabe, Fumio Tamura, and Akihiro Itai. "Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers." Journal of the American Society for Horticultural Science 127, no. 2 (2002): 262–70. http://dx.doi.org/10.21273/jashs.127.2.262.

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A total of 118 Pyrus sp. (pear) and cultivars native mainly to east Asia were subjected to randomly amplified polymorphic DNA (RAPD) analysis to evaluate genetic variation and relationships among the accessions. Two hundred fifty RAPD markers were scored from 20 decamer primers. RAPD markers specific to species were identified. Clustering analysis revealed two divisions: one comprising cultivars of P. communis L., and the other including all accessions of Pyrus native to east Asia. The grouping of the species and cultivars by RAPD data largely agrees with morphological pear taxonomy. However,

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Rahman, Md Shahidur, Md Abul Hassan, and Syeda Sharmeen Sultana. "Phylogenetic analysis among five medicinally significant Phyllanthus L. Species in Bangladesh based on taxonomic and molecular approach." Dhaka University Journal of Biological Sciences 33, no. 1 (2024): 121–31. http://dx.doi.org/10.3329/dujbs.v33i1.72490.

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A combination of taxonomical and molecular technique had done for authentic characterization of medicinally important five Phyllanthus species viz. P. acidus, P. emblica (small and large fruit form), P. niruri, P. reticulatus and P. urinaria. They were analyzed with several taxonomical parameters such as branching pattern, morphology of bark, leaves, flowers, fruits and seeds. A dichotomous bracketed key is created for easy identification of the species. RAPD analysis of five Phyllanthus species displayed that P. emblica and P. reticulatus were closely related whereas P. acidus and P. emblica

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Bieliková, L., Z. Landa, L. S. Osborne, and V. Čurn. "Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique." Plant Protection Science 38, No. 1 (2012): 1–12. http://dx.doi.org/10.17221/4813-pps.

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Entomopathogenic and mycoparasitic fungi were characterised by RAPD technique, with special attention to evaluate the genetic stability of strains that are used as active ingredients in commercial biopesticides. Strain-specific fingerprints were constructed for Paecilomyces fumosoroseus &ndash; strain PFR 97 Apopka, Gliocladium virens &ndash; strain GL 21 and Verticillium lecanii &ndash; strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM 20%WDG and bio-fungicide SoilGardTM12G that

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Thaewnon-ngiw, B., S. Klinbunga, K. Phanwichien, N. Sangduen, N. Lauhachinda, and P. Menasveta. "Genetic Diversity of Introduced (Pomacea canaliculata) and Native (PILA) Apple Snails in Thailand Revealed by Randomly Amplified Polymorphic DNA (RAPD) Analysis." ASEAN Journal on Science and Technology for Development 20, no. 3&4 (2017): 289–306. http://dx.doi.org/10.29037/ajstd.360.

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Genetic diversity of the introduced golden apple snail, Pomacea canaliculata (Lamarck, 1822) and four native apple snails; Pila ampullacea (Linneaus, 1758), P. angelica (Annandale, 1920), P. pesmei (Morelet, 1889) and P. polita (Deshayes, 1830) in Thailand were studied by RAPD analysis. Two hundred and two polymorphic fragments (180-1500 bp in length) were generated across overall investigated samples (N = 254) using three informative primers (OPA07, OPB10 and UBC122). The percentages of polymorphic bands were 98.86%, 94.56%, 90.91%, 96.94% and 95.51% for Pomacea canaliculata, P. ampullacea, P

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Boonprakob, Unaroj, and David H. Byrne. "Genetic Relationships of Diploid Plums Based on RAPD Polymorphisms." HortScience 30, no. 4 (1995): 763A—763. http://dx.doi.org/10.21273/hortsci.30.4.763a.

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Diploid plums such as Prunus salicina, P. simonii, P. cerasifera, P. americana, P. angustifolia, P. mexicana, and their hybrids have a high level of RAPD polymorphisms. Of 71 successfully used primers, there are 417 reproducible RAPD markers and only 55 (13%) markers are not polymorphic. Genetic relationships of these diploid plums based on RAPD data is estimated using genetic distance (GD) defined as GDij = 1 – Sij, where Sij is similarity coefficient. Two similarity coefficients, Jaccard's and simple matching coefficient, are compared. Simple matching always yields higher similarity coeffici

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Villar, M., F. Lefèvre, H. D. Bradshaw, and E. Teissier du Cros. "Molecular Genetics of Rust Resistance in Poplars (Melampsora larici-populina Kleb/Populus sp.) by Bulked Segregant Analysis in a 2 × 2 Factorial Mating Design." Genetics 143, no. 1 (1996): 531–36. http://dx.doi.org/10.1093/genetics/143.1.531.

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Abstract With random amplified polymorphic DNA (RAPD) markers, we have tagged a genomic region in Populus sp. involved in qualitative resistance to Melumpsora larici-populina. Our approach was based on three steps: use of RAPD markers that can be quickly and efficiently researched; application of “bulked segregant analysis” technique on individuals of one interspecific family P. trichocarpa × P. deltoides to search for RAPD markers linked to resistance; and validation of these markers in two other families linked with the first one in a 2 × 2 factorial mating design. Of five detected markers,

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Matsumoto, Sonoko, Kenta Watanabe, Hiroko Kiyota, Masato Tachibana, Takashi Shimizu, and Masahisa Watarai. "Distinction of Paramecium strains by a combination method of RAPD analysis and multiplex PCR." PLOS ONE 17, no. 3 (2022): e0265139. http://dx.doi.org/10.1371/journal.pone.0265139.

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Paramecium is employed as a valuable model organism in various research fields since a large number of strains with different characteristics of size, morphology, degree of aging, and type of conjugation can be obtained. It is necessary to determine a method for the classification and simple identification of strains to increase their utility as a research tool. This study attempted to establish a polymerase chain reaction (PCR)-based method to differentiate strains of the same species. Genomic DNA was purified from several strains of P. caudatum, P. tetraurelia, and P. bursaria used for compa

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YOUSEFI, Zeinab, Zahra TAHMASEBI, Mohammad Javad Erfani MOGHADAM, and Ali ARMINIAN. "Genetic diversity within and among two-spotted spider mite resistant and susceptible common bean genotypes." Acta agriculturae Slovenica 109, no. 3 (2017): 517. http://dx.doi.org/10.14720/aas.2017.109.3.04.

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<p>Two-spotted spider mite (<em>Tetranychus urticae </em><a title="Carl Ludwig Koch" href="https://en.wikipedia.org/wiki/Carl_Ludwig_Koch">C. L. Koch</a>, 1836), is one of the most destructive herbivores of common bean. Very little is known about the diversity among resistant sources in this crop. The present study was conducted to characterize 22 resistant and susceptible common bean genotypes by 8 Simple Sequence Repeats (SSRs) and 8 Random Amplified Polymorphic DNA (RAPD) markers. These SSR and RAPD primers produced 100 % and 81.8 % polymorphic bands. Based on

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Prasad M. P, Sushant Shekhar, and Babhulkar Amit. "Phytochemical Analysis and Antioxidant potential of Piper species and its Molecular Characterization by RAPD Markers." International Journal of Fundamental and Applied Sciences (IJFAS) 1, no. 4 (2012): 46–48. http://dx.doi.org/10.59415/ijfas.v1i4.32.

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BACKGROUD & OBJECTIVE: Piper, the pepper plants or pepper vines, are the genus of the Piperaceae family and areimportant economically and ecologically. It contains more than 1,000 species of shrubs, herbs and lianas, many of which arekeystone species in their native habitat. The diversification of this taxon is of interest to understand the evolution of plants. Inthe scope of bioactive compounds, Piper species (P. nigrum, P. retrofractum and P. longum) were screened forphytochemicals. METHODOLOGY: Phytochemical analysis of these plants showed presence of phenolic compounds,anthraquinones,

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Cavalcanti, Sarah Desirée Barbosa, José Eduardo Levi, Kátia Cristina Dantas, and José Eduardo Costa Martins. "Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis "Moore" by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing: Paracoccidioides cerebriformis revisited." Revista do Instituto de Medicina Tropical de São Paulo 47, no. 3 (2005): 119–23. http://dx.doi.org/10.1590/s0036-46652005000300001.

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Our purpose was to compare the genetic polymorphism of six samples of P. brasiliensis (113, 339, BAT, T1F1, T3B6, T5LN1), with four samples of P. cerebriformis (735, 741, 750, 361) from the Mycological Laboratory of the Instituto de Medicina Tropical de São Paulo, using Random Amplified Polymorphic DNA Analysis (RAPD). RAPD profiles clearly segregated P. brasiliensis and P. cerebriformis isolates. However, the variation on band patterns among P. cerebriformis isolates was high. Sequencing of the 28S rDNA gene showed nucleotide conservancy among P. cerebriformis isolates, providing basis for ta

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Martono, Budi, and Laba Udarno. "Keragaman Genetik Beberapa Genotipe Teh Berdasarkan Penanda RAPD (Random Amplified Polymorphic DNA)." Jurnal Tanaman Industri dan Penyegar 1, no. 1 (2014): 1. http://dx.doi.org/10.21082/jtidp.v1n1.2014.p1-6.

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<p>Informasi keragaman genetik dan ketersediaan plasma nutfah teh (Camellia sinensis) diperlukan dalam perakitan varietas unggul. Keragaman genetik berdasarkan penanda DNA dapat memberikan hasil yang lebih konsisten karena tidak dipengaruhi lingkungan. Dalam penelitian ini sebanyak 9 genotipe teh dianalisis keragamannya menggunakan enam penanda RAPD (OPA 03, OPA 05, OPB 04, OPB 06, OPC 06, dan OPD 08). Penelitian dilakukan mulai bulan Maret sampai Mei 2013 di Laboratorium Terpadu Biotrop Bogor. Perhitungan koefisien kesamaan genetik dan analisis gerombol dilakukan dengan menggunakan pera

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Silvar, C., F. Merino, and J. Díaz. "Diversity of Phytophthora capsici in Northwest Spain: Analysis of Virulence, Metalaxyl Response, and Molecular Characterization." Plant Disease 90, no. 9 (2006): 1135–42. http://dx.doi.org/10.1094/pd-90-1135.

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Phytophthora crown rot, caused by Phytophthora capsici, is potentially the most destructive disease of pepper in Spain. Phenotypic and genetic diversity of 16 P. capsici isolates collected from 11 farms in northwest Spain was characterized based on virulence, mating type, sensitivity to metalaxyl, and genetic analysis using random amplified polymorphic DNA (RAPD) methods. Low variability was observed among the isolates in their metalaxyl response, with 87.5% being highly sensitive. No isolates of the A2 mating type were detected. More variability was found in the virulence assay, and isolates

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Handayani, Titin. "Karakterisasi Sifat Ketahanan Terhadap Naungan pada Kedelai dengan Marka RAPD." Caraka Tani: Journal of Sustainable Agriculture 22, no. 1 (2018): 12. http://dx.doi.org/10.20961/carakatani.v22i1.20530.

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<p>A series of experiment of soybean tolerance to shade were conducted in order to attain the following objectives: genotypes evaluation for shade tolerance, appropriate selection method to screen tolerant genotype to shade characterization of molecular markers of shade tolerance by using RAPD marker.</p><p>The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes.</p>Molecular analysis by using RAPD technique showed tha

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Lucic, A., Snezana Mladenovic-Drinic, N. Stavretovic, et al. "Genetic diversity of Austrian pine (Pinus nigra Arnold) populations in Serbia revealed by RAPD." Archives of Biological Sciences 62, no. 2 (2010): 329–36. http://dx.doi.org/10.2298/abs1002329l.

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The ability of random amplified polymorphic DNA (RAPD) to distinguish among populations of Austrian pine from Serbia was evaluated. Ten arbitrary 10mer primers employed in the analysis produced 113 fragments of which 100 were polymorphic (88.5%). All populations had specific RAPD phenotypes. Certain rare and genotype specific bands were identified which could be effectively used to distinguish the populations. Polymorphism in RAPD markers among P. nigra population was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the

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Yang, Luquan, Md Asaduzzaman Khan, Zhiqiang Mei, et al. "Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning." Revista de Biología Tropical 62, no. 4 (2014): 1649. http://dx.doi.org/10.15517/rbt.v62i4.13493.

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<p>Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. <em>Lonicera japonica</em> is a traditionally used medicinal plant, which have been recently genetically characterized by an improved random ampliﬁed polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific <em>L. japonica</em> variety. The DNAs were extracted from fresh young leaves of

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Shibana, C., and P. Subavathy. "DETERMINATION OF GENETIC DIVERSITY IN PORTUNUS PELAGICUS (LINNAEUS, 1758) COLLECTED FROM GULF OF MANNAR." International Journal of Advanced Research 9, no. 03 (2021): 409–20. http://dx.doi.org/10.21474/ijar01/12598.

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In the present study, the marine crab Portunuspelagicus was screened with 17 primers and made to score polymorphism. The main emphasis of the present study was to assess the genetic diversity at intra specific level among the 3 accessions of P. pelagicus species of Gulf of Mannarusing RAPD markers. RAPD analysis shows that there is a high level of polymorphism among different accessions. From this study, it was understood that each location varied with respect to environmental factors and genetic parameters. The OPB-18, OPB-19, OPC-07 and OPN-06 primers produced distinct, highly reproducible a

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Riederer, Kathleen M., Jambunathan Ramanathan, Jeff Barczak, Joseph Baran, Jr., and Riad Khatib. "Utility of a pre-optimized kit for random amplified polymorphic DNA in typingCandida albicans." Canadian Journal of Microbiology 48, no. 4 (2002): 369–73. http://dx.doi.org/10.1139/w02-018.

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The utility of a pre-optimized kit for random amplified polymorphic DNA (RAPD) was assessed in typing diverse strains of Candida albicans from epidemiologically unrelated inpatients (interpatient analysis) and in detecting clonal variations that maybe present within individual patient isolates (intrapatient analysis). Stool samples from inpatients were cultured on Inhibitory Mold agar. Nine individual colonies from all patients with [Formula: see text]9 colonies of C. albicans (n = 18) were selected, frozen, and karyotyped using CHEF genomic DNA plug kits and CHEF-DRIII. Each of the selected c

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Nandariyah, Nandariyah, and Endang Yuniastuti. "Identication and Classification of Menoreh Durians Based on Molecular - RAPD Markers." Journal of Biodiversity and Biotechnology 2, no. 1 (2022): 1. http://dx.doi.org/10.20961/jbb.v2i1.59401.

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<p>Menoreh durian is an accessions durian fruit originally from Kulonprogo district, Special Region of Yogyakarta, Indonesia. In the context of proposing Menoreh durian to be a new superior variety, it is necessary to have an identity and position in the molecular genetic group. The aim of the study was to obtain the identity and position of the of Menoreh Durian in the molecular genetic classification. The wet laboratory experiment was using PCR-based RAPD method. Eleven accessions of durian were identified and characterized then grouped based on molecular analysis which were pink Menor

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S. PANDIN, DONATA. "KERAGAMAN GENETIK KELAPA DALAM BALI (DBI) DAN DALAM SAWARNA (DSA) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)." Jurnal Penelitian Tanaman Industri 16, no. 2 (2020): 83. http://dx.doi.org/10.21082/jlittri.v16n2.2010.83-89.

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<p>ABSTRACT</p><p>Keragaman genetik dan hubungan kekerabatan dalam populasikelapa Dalam Bali (DBI) dan Dalam Sawarna (DSA) dianalisismenggunakan penanda RAPD. Penelitian dilaksanakan di LaboratoriumBiologi Tumbuhan, Pusat Penelitian Sumber Daya Hayati danBioteknologi, Institut Pertanian Bogor pada Februari-Mei 2007. Bahanyang digunakan dalam penelitian sebanyak 10 individu dari masing-masing populasi. Primer acak digunakan dalam analisis terdiri atas 10primer -10 mer yaitu OPA-02, OPA-08, OPA-10, OPA-13, OPA-20,OPB-08, OPB-11, OPB-12, OPB-15, OPB-20. DNA diekstraksimenggunaka

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Svitashev, Sergei, Tomas Bryngelsson, Xiaomei Li, and Richard RC Wang. "Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus." Genome 41, no. 1 (1998): 120–28. http://dx.doi.org/10.1139/g97-108.

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We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St, Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 2

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Speijer, Han, Paul H. M. Savelkoul, Marc J. Bonten, Ellen E. Stobberingh, and Jeroen H. T. Tjhie. "Application of Different Genotyping Methods forPseudomonas aeruginosa in a Setting of Endemicity in an Intensive Care Unit." Journal of Clinical Microbiology 37, no. 11 (1999): 3654–61. http://dx.doi.org/10.1128/jcm.37.11.3654-3661.1999.

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Colonization with Pseudomonas aeruginosa was studied by taking serial swab specimens from the oropharynges and anuses and tracheal and gastric aspirates from patients in an intensive care unit during a 10-month period in a setting of endemicity. Nineteen (10%) of the 192 patients included in the study were colonized on admission, while another 30 (16%) patients acquired P. aeruginosawhile in the hospital. Typing of 353 isolates was performed by random amplified polymorphic DNA (RAPD) analysis, and 56 strains were selected for further typing by RAPD analysis, pulsed-field gel electrophoresis (P

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Kovacevic, Dragan, Biljana Nikolic, Snezana Mladenovic-Drinic, et al. "Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers." Genetika 45, no. 2 (2013): 493–502. http://dx.doi.org/10.2298/gensr1302493k.

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Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD

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CATALÁN, PILAR, YING SHI, LAUREL ARMSTRONG, JOHN DRAPER, and CLIVE A. STACE. "Molecular phylogeny of the grass genus Brachypodium P. Beauv. based on RFLP and RAPD analysis." Botanical Journal of the Linnean Society 117, no. 4 (1995): 263–80. http://dx.doi.org/10.1111/j.1095-8339.1995.tb02590.x.

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Stringari, Danyelle, Chirlei Glienke, Daniel de Christo, Walter Maccheroni Jr., and João Lucio de Azevedo. "High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the citrus black spot." Brazilian Archives of Biology and Technology 52, no. 5 (2009): 1063–73. http://dx.doi.org/10.1590/s1516-89132009000500002.

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RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies h

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