Academic literature on the topic 'Real time Q-PCR'

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Journal articles on the topic "Real time Q-PCR"

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Crow, Robert M., Jill S. Gartland, Angela T. McHugh, and Kevan M. A. Gartland. "Real-time GUS analysis using Q-PCR instrumentation." Journal of Biotechnology 126, no. 2 (2006): 135–39. http://dx.doi.org/10.1016/j.jbiotec.2006.04.018.

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Zhang, Wenju, Yulei Zhao, Qingjin Xu, and Qin Chen. "Development of a triplex real-time PCR for simultaneous detection of allergenic ingredients in processed food." Czech Journal of Food Sciences 36, No. 1 (2018): 22–27. http://dx.doi.org/10.17221/28/2017-cjfs.

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SYBR Green real-time or quantitative PCR (Q-PCR) is a suitable system in which to establish a multiplex method to detect allergenic ingredients in food. In this study, a triplex Q-PCR method was developed to detect trace amounts of peanut, soybean and sesame in processed food. Specific PCR primer sets were designed and the concentration of the primers used in the triplex PCR was optimised. The triplex method showed high specificity and sensitivity which were similar to those of the simplex method, and it was applied for the detection of allergenic ingredients in commercially available processe
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Bohrer, C. L., X. Tao, E. L. Torpey, D. Taylor, R. T. Scott, and N. R. Treff. "Detection of contamination by quantitative real-time (q)PCR." Fertility and Sterility 100, no. 3 (2013): S206. http://dx.doi.org/10.1016/j.fertnstert.2013.07.1375.

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Simon, P. "Q-Gene: processing quantitative real-time RT-PCR data." Bioinformatics 19, no. 11 (2003): 1439–40. http://dx.doi.org/10.1093/bioinformatics/btg157.

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Gallagher, Robert E. "Prime-time for real-time Q-RT-PCR in good-prognosis AML?" Blood 102, no. 8 (2003): 2709–10. http://dx.doi.org/10.1182/blood-2003-08-2660.

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Schneeberger, Peter M., Mirjam H. A. Hermans, Erik J. van Hannen, Jeroen J. A. Schellekens, Alexander C. A. P. Leenders, and Peter C. Wever. "Real-Time PCR with Serum Samples Is Indispensable for Early Diagnosis of Acute Q Fever." Clinical and Vaccine Immunology 17, no. 2 (2009): 286–90. http://dx.doi.org/10.1128/cvi.00454-09.

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ABSTRACT The world's largest Q fever outbreak is ongoing in The Netherlands with around 3,000 confirmed cases since the first half of 2007. Increased awareness has resulted in early referral of patients for diagnostics. An important drawback to serological diagnosis of acute Q fever is the lag phase in antibody response. Therefore, we evaluated the performance of a real-time PCR for detection of Coxiella burnetii DNA using serum samples from patients with acute Q fever. PCR, targeting IS1111, was retrospectively performed on acute-phase and follow-up convalescent-phase serum samples from 65 pa
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Khan, Izhar U. H., Alyssa Loughborough, and Thomas A. Edge. "DNA-based real-time detection and quantification of aeromonads from fresh water beaches on Lake Ontario." Journal of Water and Health 7, no. 2 (2009): 312–23. http://dx.doi.org/10.2166/wh.2009.041.

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The present study was designed to develop a novel, rapid, direct DNA-based protocol to enumerate aeromonads in recreational waters. An Aeromonas genus-specific real-time quantitative polymerase chain reaction (Q-PCR) protocol was developed and optimized using newly designed genus-specific oligonucleotide primers derived from the gyrase B subunit (GyrB) gene. A standard curve was developed based on the PCR protocol with a minimum quantification limit of 10 cell equivalents ml−1 achieved using an autoclaved water sample from recreational water spiked with known quantities of an Aeromonas ATCC st
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Albuquerque, Yvana Maria Maia de, Ana Luiza Magalhães de Andrade Lima, Ana Kelly Lins, Marcelo Magalhães, and Vera Magalhães. "QUANTITATIVE REAL-TIME PCR (Q-PCR) FOR SPUTUM SMEAR DIAGNOSIS OF PULMONARY TUBERCULOSIS AMONG PEOPLE WITH HIV/AIDS." Revista do Instituto de Medicina Tropical de São Paulo 56, no. 2 (2014): 139–42. http://dx.doi.org/10.1590/s0036-46652014000200009.

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Objective: To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV/AIDS with a clinical suspicion of PTB.Method: This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, attended at two referral hospitals for people living with HIV/AIDS in the city of Recife, Pernambuco, Brazil. A Löwenstein-Jensen medium culture and 7H9 broth were used as gold standard.Results: Of the 140
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Rodr�guez-L�zaro, David, Deborah A. Lewis, Alain A. Ocampo-Sosa, et al. "Internally Controlled Real-Time PCR Method for Quantitative Species-Specific Detection and vapA Genotyping of Rhodococcus equi." Applied and Environmental Microbiology 72, no. 6 (2006): 4256–63. http://dx.doi.org/10.1128/aem.02706-05.

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ABSTRACT We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an internal amplification control (IAC) for identification of false negatives. A second Q-PCR targeted the virulence plasmid gene vapA, carried by most horse isolates but infrequently found in isolates from other sources. The choE-IAC and vapA assays were 100% sensitive and
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Abbas, Adeeb, Majeed A. Sabbah, Abdul-salam Hatam, Luma A. Yasser, and Baan Abdul-Latif. "Molecular diagnosis of Iraqi chronic myeloid leukemia patients using quantitative real-time PCR." Journal of Biotechnology Research Center 4, no. 2 (2010): 64–69. http://dx.doi.org/10.24126/jobrc.2010.4.2.125.

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hronic myeloid leukemia (CML), also known as chronic granulocytic leukemia, is a form of leukemia characterized by the increased and unregulated growth of myeloid cells in the bone marrow and the accumulation of these cells in peripheral blood. Total RNA extraction, cDNA and quantitative real-time PCR (Q-RT-PCR) were done for thirty CML Iraqi patients. Hematology tests (hemoglobin, platelets and WBCs counts) were done for the same samples in the same time. The results of this study show that some samples with normal hematology values have BCR-ABL (p210) fusion transcript with molecular analysi
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Dissertations / Theses on the topic "Real time Q-PCR"

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Penna, Sergio D., and Domingos B. Rios. "MIGRATING FROM A VAX/VMS TO AN INTEL/WINDOWS-NT BASED GROUND STATION." International Foundation for Telemetering, 1999. http://hdl.handle.net/10150/608310.

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International Telemetering Conference Proceedings / October 25-28, 1999 / Riviera Hotel and Convention Center, Las Vegas, Nevada<br>Upgrading or replacing production systems is always a very resource-consuming task, in particular if the systems being replaced are quite specialized, such as those serving any Flight Test Ground Station. In the recent past a large number of Ground Station systems were based in Digital’s VAX/VMS architecture. The computer industry then expanded very fast and by 1990 realtime PCM data processing systems totally dependent on hardware and software designed for IBM-PC
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Book chapters on the topic "Real time Q-PCR"

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Wu, Finch C. T., Oscar N. J. Hong, Amy J. C. Trappey, and Charles V. Trappey. "VR-Enabled Chatbot System Supporting Transformer Mass-Customization Services." In Advances in Transdisciplinary Engineering. IOS Press, 2020. http://dx.doi.org/10.3233/atde200088.

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Chatbot is a conversational question answering (Q&amp;A) system capable of natural language communication between a computer system and a person. The use of chatbots for 24-hour customer service provides quick responses that solve problems online. This approach is quickly becoming a convenient way for companies to enhance their customer services without location or knowledgeable staff limitations. This research proposes a system framework and develops a prototype virtual reality (VR) enabled transformer mass-customization consultation chatbot. The chatbot technique is a retrieval-based intelli
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Conference papers on the topic "Real time Q-PCR"

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Kubista, Mikael, Anders Stalberg, and Tzachi Bar. "Light-up-probe-based real-time Q-PCR." In BiOS 2001 The International Symposium on Biomedical Optics, edited by Ramesh Raghavachari and Weihong Tan. SPIE, 2001. http://dx.doi.org/10.1117/12.424589.

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Wang, Xiaokun, Hang Wang, Qiang Deng, and Renyi Xu. "State Monitoring of Electric Gate Valve of Nuclear Power Plant Based on Kernel Principal Component Analysis and Jensen-Shannon Divergence." In 2022 29th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/icone29-92733.

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Abstract With the rapid development of the global nuclear power industry, the safety of nuclear power equipment has received increasing attention. In the event of an accident in its equipment, it is likely to cause significant losses and harm to personnel and the environment. Therefore, real-time monitoring of nuclear power plants has very important practical significance. Based on Kernel Principal Component Analysis (KPCA) and Jensen-Shannon (JS) divergence, equipment-level state monitoring of electric gate valves of nuclear power plants will be conducted. The traditional Principal Component
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Sorensen, Ole, Yang Wu, Didier Caillon, et al. "Successfully Adapting and Overcoming Challenges for Coiled Tubing Applications in High-Pressure Sour Environment and Horizontal Unconventional Tight Gas Wells in UAE." In SPE/ICoTA Well Intervention Conference and Exhibition. SPE, 2022. http://dx.doi.org/10.2118/209032-ms.

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Abstract During the completion phase of four unconventional wells in the United Arab Emirates (UAE), a detailed engineering approach enabled overcoming challenges presented by the extreme conditions of pressure, temperature, and sour environment across long horizontal sections to successfully carry out cleanout activities. The methods implemented to address those conditions prioritized personnel safety and asset integrity. The unconventional wells in this campaign were characterized by a reservoir pressure of approximately 13,000 psi and a bottomhole temperature of approximately 325°F. Gas was
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Speed, Jonathon. "Demystifying chemometrics: how multivariate analysis allows spectroscopy to be used to solve most analytical problems." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/pkrn4677.

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Chemists have been using spectroscopic techniques for decades if not hundreds of years. The large range of different physical transitions nuclei and molecules can undertake when irradiate with specific wavelengths of light means that almost every property of interest can and has been studied by spectroscopic means. However, the need to interpret the raw spectra means only specialists are able to interpret the fundamental information present in a spectrum, turning spectroscopy into a tool for experts. The advent of chemometrics meant that spectrometers could be changed into concentration meters
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Reports on the topic "Real time Q-PCR"

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Ghanim, Murad, Joe Cicero, Judith K. Brown, and Henryk Czosnek. Dissection of Whitefly-geminivirus Interactions at the Transcriptomic, Proteomic and Cellular Levels. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592654.bard.

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Our project focuses on gene expression and proteomics of the whitefly Bemisia tabaci (Gennadius) species complex in relation to the internal anatomy and localization of expressed genes and virions in the whitefly vector, which poses a major constraint to vegetable and fiber production in Israel and the USA. While many biological parameters are known for begomovirus transmission, nothing is known about vector proteins involved in the specific interactions between begomoviruses and their whitefly vectors. Identifying such proteins is expected to lead to the design of novel control methods that i
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